Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| markov chain approach to analyze the dynamics of pathogen fecal shedding--example of listeria monocytogenes shedding in a herd of dairy cattle. | fecal shedding is an important mechanism of spreading of a number of human and animal pathogens. understanding of the dynamics of pathogen fecal shedding is critical to be able to control or prevent the spread of diseases caused by these pathogens. the objective of this study was to develop a model for analysis of the dynamics of pathogen fecal shedding. fecal shedding of listeria monocytogenes in dairy cattle was used as a model system. a markov chain model (mcm) with two states, shedding and n ... | 2007 | 17092523 |
| partial purification and characterization of protease of bacillus proteolyticus cfr3001 isolated from fish processing waste and its antibacterial activities. | bacillus proteolyticus cfr3001 isolated from fish processing wastes (both fresh water and marine) produced an alkaline protease. the optimum conditions for cell growth and protease production were 37 degrees c, 96 h, agitation speed of 100 rpm and medium ph 9. the partially purified protease obtained from had specific activity of 22.05 at 37 degrees c was active between 40 degrees c and 50 degrees c and lost >20% of its activity around 60 degrees c. its molecular weight was approximately 29 kda ... | 2007 | 17092708 |
| an important step in listeria lipoprotein research. | 2007 | 17071755 | |
| proteinase-activated receptors induce nonoxidative, antimicrobial peptides and increased antimicrobial activity in human mononuclear phagocytes. | as thrombin and sfllrnpndkyepf (sfllrn-14), a synthetic ligand, mainly of the proteinase-activated receptor-1 (par-1), induce in monocytes the synthesis and secretion of chemokines, the par pathway can be viewed as a mononuclear phagocyte-activating principle. classically, antimicrobial activity of mononuclear phagocytes is the measure for activation. here, we investigated whether thrombin or sfllrn-14 increases the antimicrobial activity of human monocytes and compared these effects to those of ... | 2007 | 17095611 |
| estimation of microbial contamination of food from prevalence and concentration data: application to listeria monocytogenes in fresh vegetables. | a normal distribution and a mixture model of two normal distributions in a bayesian approach using prevalence and concentration data were used to establish the distribution of contamination of the food-borne pathogenic bacteria listeria monocytogenes in unprocessed and minimally processed fresh vegetables. a total of 165 prevalence studies, including 15 studies with concentration data, were taken from the scientific literature and from technical reports and used for statistical analysis. the pre ... | 2007 | 17098926 |
| adjuvant properties of listeriolysin o protein in a dna vaccination strategy. | the use of infectious agents as vaccine adjuvants has shown utility in both prophylactic and therapeutic vaccinations. listeria monocytogenes has been used extensively as a vaccine vehicle due to its ability to initiate both cd4(+) and cd8(+) immune responses. previous work from this laboratory has used transgenic listeria to deliver vaccine constructs. a chimeric protein composed of tumor antigen and a non-hemolytic variant of the listeria protein, listeriolysin o (llo), has demonstrated effect ... | 2007 | 17102978 |
| more than one variant of listeria monocytogenes isolated from each of two human cases of invasive listeriosis. | two variants of listeria monocytogenes were isolated from blood cultures from each of two patients with listeriosis. each variant displayed a two-band difference in dna profile from the other by pulsed-field gel electrophoresis. although this difference in profile is insufficient to distinguish clearly between the variants, the possibility of co-infection with different strains of l. monocytogenes needs to be considered. we suggest that more than one colony should be selected for molecular typin ... | 2007 | 17109771 |
| role of branched-chain fatty acids in ph stress tolerance in listeria monocytogenes. | in alkaline conditions, listeria monocytogenes cells develop higher proportions of branched-chain fatty acids (fas), including more anteiso forms. in acid conditions, the opposite occurs. reduced growth of ph-sensitive mutants at adverse ph (5.0/9.0) was alleviated by the addition of 2-methylbutyrate (an anteiso-fa precursor), suggesting that anteiso-fas are important in adaptation to adverse ph. the balance between anteiso- and iso-fas may be more important than changes in the amounts and/or de ... | 2007 | 17114323 |
| in the fvb/n her-2/neu transgenic mouse both peripheral and central tolerance limit the immune response targeting her-2/neu induced by listeria monocytogenes-based vaccines. | listeria monocytogenes-based vaccines for her-2/neu are capable of breaking tolerance in fvb/n rat her-2/neu transgenic mice. the growth of implanted nt-2 tumors, derived from a spontaneously occurring tumor in the fvb/n her-2/neu transgenic mouse, was significantly slower in these mice following vaccination with a series of l. monocytogenes-based vaccines for her-2/neu. mechanisms of t cell tolerance that exist in these transgenic mice include the absence of functional high avidity anti-her-2/n ... | 2007 | 17131121 |
| stage of primary infection with lymphocytic choriomeningitis virus determines predisposition or resistance of mice to secondary bacterial infections. | we investigated the effect of a primary non-lethal infection with lymphocytic choriomeningitis virus (lcmv) on the course and outcome of a secondary infection with the gram-negative salmonella enterica serovar typhimurium or the gram-positive listeria monocytogenes in mice. we found that at each stage of the viral infection the susceptibility of mice to bacterial super-infections changes dramatically and depends also on whether the secondary infection is a gram-positive or gram-negative one. the ... | 2007 | 17136407 |
| a fret analysis to unravel the role of cholesterol in rac1 and pi 3-kinase activation in the inlb/met signalling pathway. | the signalling pathway for the hepatocyte growth factor receptor, met/hgf-r, is hijacked by the bacterial surface protein inlb to induce listeria monocytogenes entry into non-phagocytic cells. we previously showed that listeria invades host cells by interacting with specialized microdomains of the host plasma membrane called lipid rafts. in this study, we analysed in living cells signalling events that are crucial for listeria entry using a fluorescence resonance energy transfer-based microscopi ... | 2007 | 17140407 |
| modeling the growth of listeria monocytogenes based on a time to detect model in culture media and frankfurters. | in the current study, temperature dependent growth of listeria monocytogenes strain h7776, with an initial inoculum level of approximately 0.1 cfu/ml or g, was modeled based on "time to detect" (ttd) using the arrhenius equation. the activation energies (e(a)) obtained from specific growth rate and lag phase duration in tryptic soy broth (tsb) and frankfurters were compared. at 4 degrees c, the ttd for l. monocytogenes was 217 h in tsb and 48 h in portions of frankfurters. the ttd decreased as t ... | 2007 | 17140686 |
| management of microbiological safety of ready-to-eat meat products by mathematical modelling: listeria monocytogenes as an example. | the recent commission regulation (ec) no 2073/2005 establishes microbiological criteria in foods. for the pathogen listeria monocytogenes in the category ready-to-eat foods able to support its growth, other than those intended for infants and for special medical purposes, two different microbiological criteria are proposed: (i) l. monocytogenes levels should be <100 cfu/g throughout the shelf-life of the product, (ii) absence in 25 g of the product at the stage before the food has left the immed ... | 2007 | 17140689 |
| a case of listeria meningitis associated with increased adenosine deaminase in cerebrospinal fluid. | we present, to the best of our knowledge, the first case of listeria meningitis with increased adenosine deaminase (ada) in cerebrospinal fluid. although some cases of other etiologies were also reported, this report informs those who manage patients with meningitis that not only mycobacterium and brucella spp. but also other facultative agents can increase ada levels in cerebrospinal fluid, possibly through monocyte-macrophage activation. | 2007 | 17141462 |
| differentiation of listeria monocytogenes serovars by using artificial neural network analysis of fourier-transformed infrared spectra. | a classification system based on fourier transform infrared (ftir) spectroscopy combined with artificial neural network analysis was designed to differentiate 12 serovars of listeria monocytogenes using a reference database of 106 well-defined strains. external validation was performed using a test set of another 166 l. monocytogenes strains. the o antigens (serogroup) of 164 strains (98.8%) could be identified correctly, and h antigens were correctly determined in 152 (91.6%) of the test strain ... | 2007 | 17142376 |
| persistent antigen presentation after acute vesicular stomatitis virus infection. | long-term antigen expression is believed to play an important role in modulation of t-cell responses to chronic virus infections. however, recent studies suggest that immune responses may occur late after apparently acute infections. we have now analyzed the cd8 t-cell response to vesicular stomatitis virus (vsv), which is thought to cause to an infection characterized by rapid virus clearance by innate and adaptive immune system components. unexpectedly, virus-encoded antigen was detectable mor ... | 2007 | 17151119 |
| six-color segmentation of multicolor images in the infection studies of listeria monocytogenes. | multiple immunofluorescent staining is a powerful strategy for visualizing the spatial and temporal relationship between antigens, cell populations, and tissue components in histological sections. to segment different cell populations from the multicolor image generated by immunostaining based on color addition theory, a systems approach is proposed for automatic segmentation of six colors. after image acquisition and processing, images are automatically segmented with the proposed approach and ... | 2007 | 17177276 |
| hyponatraemia in adults with community-acquired bacterial meningitis. | hyponatraemia in adults with bacterial meningitis has been described as a common complication, but its true prevalence and clinical importance are unknown. | 2007 | 17178734 |
| microbial composition in bioaerosols of a high-throughput chicken-slaughtering facility. | the microbial composition of the air in various areas of a high-throughput chicken-slaughtering facility was investigated. over a 4-mo period, 6 processing areas were sampled, and the influence of environmental factors was monitored. the highest counts of microorganisms were recorded in the initial stages of processing, comprising the receiving-killing and defeathering areas, whereas counts decreased toward the evisceration, air-chilling, packaging, and dispatch areas. maximum microbial counts w ... | 2007 | 17179429 |
| marination of turkey breast fillets to control the growth of listeria monocytogenes and improve meat quality in deli loaves. | turkey deli loaves were evaluated using organic marinades in the raw product to control the growth of listeria monocytogenes (lm) and improve meat quality in the cooked product. treatments included sodium tripolyphosphate (stp; 0.45%, control), sodium lactate (3%), sodium diacetate (0.25%), sodium citrate (0.75%), and sodium lactate (3%)/sodium diacetate (0.25%) combination, all containing 1.5% salt. data collected in the 2 trials included ph; lightness, redness, and yellowness; bind ability; co ... | 2007 | 17179430 |
| genotypic characterization of listeria spp. isolated from fresh water fish. | a total of 200 samples (muscles and viscera, 100 of each) of fresh water fish, walking catfish (clarias batrachus) were screened for listeria spp. all the samples were subjected to a two-step enrichment followed by plating on selective media. confirmation of the isolates was on the basis of biochemical characters, haemolysis on blood agar and christie, atkins, munch petersen test. a total of 39 isolates of listeria spp. were recovered. of these 26 (67%), 8 (21%), 3 (8%) and 2 (5%) were listeria ... | 2007 | 17182144 |
| activation of type b t cells after protein immunization reveals novel pathways of in vivo presentation of peptides. | type b t cells recognize a peptide-mhc conformer generated in recycling endosomes and eliminated by h2-dm in late endosomes; as a result, they recognize exogenous peptide, but fail to respond to the identical epitope generated from the native protein. to investigate the behavior of these cells in vivo, we generated mice transgenic for a type b tcr recognizing the 48-62 epitope of hen egg white lysozyme (hel) presented by i-a(k). type b t cells responded only to peptide ex vivo, but responded in ... | 2007 | 17182547 |
| the microbiological quality of hot water-washed broccoli florets and cut green beans. | to determine the effect of hot water washing on the microbiological quality of cut broccoli florets and trimmed green beans. | 2007 | 17184318 |
| partial characterization of bacteriocins produced by human lactococcus lactis and pediococccus acidilactici isolates. | the aim of this study was to isolate bacteriocin-producing lactic acid bacteria (lab) from human intestine. | 2007 | 17184344 |
| influence of ph, water activity and acetic acid concentration on listeria monocytogenes at 7 degrees c: data collection for the development of a growth/no growth model. | growth/no growth models can be used to determine the chance that microorganisms will grow in specific environmental conditions. as a consequence, these models are of interest in the assessment of the safety of foods which can be contaminated with food pathogens. in this paper, growth/no growth data for listeria monocytogenes (in a monoculture and in a mixed strain culture) are presented. the data were gathered at 7 degrees c in nutrient broth with different combinations of environmental factors ... | 2007 | 17184866 |
| the mutations lys 114 --> gln and asp 126 --> asn disrupt an intersubunit salt bridge and convert listeria innocua dps into its natural mutant listeria monocytogenes dps. effects on protein stability at low ph. | the stability of the dodecameric listeria monocytogenes dps has been compared with that of the listeria innocua protein. the two proteins differ only in two amino acid residues that form an intersubunit salt-bridge in l. innocua dps. this salt-bridge is replaced by a hydrogen bonding network in l. monocytogenes dps as revealed by the x-ray crystal structure. the resistance to low ph and high temperature was assayed for both dps proteins under equilibrium conditions and kinetically. despite the i ... | 2007 | 17186524 |
| enteropathogenic escherichia coli, shigella flexneri, and listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals. | enteropathogenic escherichia coli, shigella flexneri, and listeria monocytogenes induce localized actin polymerization at the cytoplasmic face of the plasma membrane or within the host cytoplasm, creating unique actin-rich structures termed pedestals or actin tails. the process is known to be mediated by the actin-related protein 2 and 3 (arp2/3) complex, which in these cases acts downstream of neural wiskott-aldrich syndrome protein (n-wasp) or of a listerial functional homolog of wasp family p ... | 2007 | 17118974 |
| acta is required for crossing of the fetoplacental barrier by listeria monocytogenes. | the facultative intracellular bacterial pathogen listeria monocytogenes induces severe fetal infection during pregnancy. little is known about the molecular mechanisms allowing the maternofetal transmission of bacteria. in this work, we studied fetoplacental invasion by infecting mice with various mutants lacking virulence factors involved in the intracellular life cycle of l. monocytogenes. we found that the placenta was highly susceptible to bacteria, including avirulent bacteria, such as an l ... | 2007 | 17118980 |
| the role of the activated macrophage in clearing listeria monocytogenes infection. | macrophage activation often contributes to the strong immune response elicited upon infection. the ability of macrophages to become activated was discovered when sub-lethal primary infections of mice with the bacterium listeria monocytogenes provided protection against secondary infections through non-humoral immunity. l. monocytogenes infect and propagate in macrophages by escaping the phagosome into the cytosol, where they avoid humoral immune mediators. activated macrophages kill l. monocytog ... | 2007 | 17127272 |
| improved antibacterial host defense and altered peripheral granulocyte homeostasis in mice lacking the adhesion class g protein receptor cd97. | cd97 is a member of the adhesion family of g protein-coupled receptors. alternatively spliced forms of cd97 bind integrins alpha5beta1 and alphavbeta3, decay accelerating factor, or dermatan sulfate. cd97 is expressed on myeloid cells at high levels and a variety of other cell types at lower levels. little is known about the physiological function of cd97. to begin dissecting the function of cd97, we evaluated the immune response of cd97 null mice to systemic infection by listeria monocytogenes. ... | 2007 | 17158902 |
| characterization of early gamma interferon (ifn-gamma) expression during murine listeriosis: identification of nk1.1+ cd11c+ cells as the primary ifn-gamma-expressing cells. | though it is well established that gamma interferon (ifn-gamma) is crucial to the early innate defense of murine listeriosis, its sources remain controversial. in this study, intracellular cytokine staining of ifn-gamma-expressing splenocytes early after listeria monocytogenes infection revealed that nk1.1(+), cd11c(+), cd8(+) t, and cd4(+) t cells expressed ifn-gamma 24 h after infection. contrary to the previous report, most ifn-gamma(+) dendritic cells (dc) were cd8alpha(-) dc. unexpectedly, ... | 2007 | 17158904 |
| the adaptor protein card9 is required for innate immune responses to intracellular pathogens. | the caspase-recruitment domain-containing adaptor protein card9 regulates the innate signaling responses to fungal infection. here we show that card9 is required for innate immune responses against intracellular pathogens. we generated card9(-/-) mice and found that card9-deficient macrophages had defects in activation of the kinases p38 and jnk but not of transcription factor nf-kappab after bacterial and viral infection. card9-deficient mice failed to clear infection and showed altered cytokin ... | 2007 | 17187069 |
| use of linear, weibull, and log-logistic functions to model pressure inactivation of seven foodborne pathogens in milk. | survival curves of six foodborne pathogens suspended in ultra high-temperature (uht) whole milk and exposed to high hydrostatic pressure at 21.5 degrees c were obtained. vibrio parahaemolyticus was treated at 300 mpa and other pathogens, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica serovar enteritidis, salmonella enterica serovar typhimurium, and staphylococcus aureus were treated at 600 mpa. all the survival curves showed a rapid initial drop in bacterial counts followe ... | 2007 | 17188197 |
| growth of listeria monocytogenes in egg salad and pasta salad formulated with mayonnaise of various ph and stored at refrigerated and abuse temperatures. | this study investigated and modeled the behavior of listeria monocytogenes in egg salad and pasta salad as affected by mayonnaise ph (3.8, 4.2, 4.6, and 5.0) and storage temperature (4, 8, and 12 degrees c). at each storage temperature, l. monocytogenes was able to grow in both salads regardless of the mayonnaise ph. the lag-phase durations (lpd) of l. monocytogenes in egg salad ranged from 33 to 85, 15 to 50, and 0 to 19 h, and the growth rates (gr) ranged from 0.0187 to 0.0318, 0.0387 to 0.051 ... | 2007 | 17188199 |
| phenotypic and technological diversity of lactic acid bacteria and staphylococci isolated from traditionally fermented sausages in southern greece. | the physicochemical and microbiological characteristics of spontaneously fermented sausages made by two medium-sized enterprises (mse) located in southern greece have been studied. a total of 300 lactic acid bacteria and 300 staphylococcal strains have been isolated and identified by their physiological characteristics. lactobacillus plantarum strains were found to dominate the lactic acid bacteria microbiota in most of the cases with l. sakei strains prevailing in some of them and l. rhamnosus ... | 2007 | 17188204 |
| effect of industrial and natural biocides on spoilage, pathogenic and technological strains grown in biofilm. | this study aimed at investigating bactericide solutions effective on spoilage and pathogenic bacteria while preserving technological bacteria. two compounds of essential oil (thymol and eugenol), one essential oil of satureja thymbra and two industrial biocides (pe 270-30, brillo) were tested on technological strains (staphylococcus equorum, staphylococcus succinus and lactobacillus sakei) grown in monoculture biofilm and on a mixed biofilm of pathogenic bacteria (staphylococcus aureus, listeria ... | 2007 | 17188206 |
| antimicrobial and cytotoxic knotwood extracts and related pure compounds and their effects on food-associated microorganisms. | knotwood or bark extracts prepared from 30 species of hard and soft wood trees as well as selected pure compounds (lignans, stilbenes and flavonoids) were assayed for their antimicrobial activity against a battery of both gram positive and negative bacteria, yeasts, and filamentous fungi (bacillus cereus, staphylococcus aureus, listeria monocytogenes, lactobacillus plantarum, escherichia coli, salmonella infantis, pseudomonas fluorescens, candida albicans, saccharomyces cerevisiae, aspergillus f ... | 2007 | 17188387 |
| genetic diversity of listeria monocytogenes recovered from infected persons and pork, seafood and dairy products on retail sale in france during 2000 and 2001. | growth of the food-borne human pathogen listeria monocytogenes to large numbers in ready-to-eat food products greatly increases the risk of disease for susceptible consumers. a better knowledge of the population structure of l. monocytogenes present in retailed food could allow better prevention strategies to be developed. we present the analysis of 450 l. monocytogenes isolates, 179 responsible for sporadic human cases of listeriosis and 271 isolated from foods collected from retailers. all iso ... | 2007 | 17188773 |
| effect of preinoculation growth media and fat levels on thermal inactivation of a serotype 4b strain of listeria monocytogenes in frankfurter slurries. | preinoculation growth conditions and fat levels were evaluated for effects on the heat resistance of listeria monocytogenes strain mfs 102 in formulated frankfurter slurries and on frankfurter surfaces. comparison of linear inactivation rates (d-values) for cells heated in frankfurter slurry showed that growth conditions were significant (p<0.05) factors affecting subsequent thermal resistance. the average d(60 degrees c)-values for the five preinoculation growth media tested from most resistant ... | 2007 | 17189761 |
| modelling thermal inactivation of listeria monocytogenes in sucrose solutions of various water activities. | the heat resistance of listeria monocytogenes was determined in sucrose solutions with water activity (a(w)) ranging from 0.99 to 0.90. at all temperatures investigated shape of the survival curves depended on the a(w) of the treatment medium. the survival curves for a(w)=0.99 appeared to be linear, for a(w)=0.96 were slightly upwardly concaved and for a(w)=0.93 and 0.90 were markedly concave upward. a mathematical model based on the weibull distribution provided a good fit for all the survival ... | 2007 | 17189763 |
| assessing biofilm formation by listeria monocytogenes strains. | when a microtitre plate assay was used to quantify biofilm production by listeria monocytogenes strains following growth in tryptone soy broth (tsb) for 48 h at 20 degrees c, 127 of 138 strains (92.0%) were classified as weak, 9 of 138 strains (6.5%) as moderate and only 2 of 138 strains (1.5%) as strong biofilm formers. the strains included environmental, animal, food (persistent and sporadic strains) and clinical isolates previously typed using esterase electrophoresis (ese) and multi-locus en ... | 2007 | 17189764 |
| pulsed-field gel electrophoresis (pfge) analysis of temporally matched listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments reveals source-associated as well as widely distributed pfge types. | a total of 495 temporally and geographically matched listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments were used to investigate l. monocytogenes pulsed-field gel electrophoresis (pfge) type diversity. two-enzyme (asci and apai) pfge discriminated 310 pfge types and exhibited higher overall discriminatory power (simpson's index of discrimination [d] = 0.995) than either ecori ribotyping (d = 0.950) or asci or apai single-enzyme pf ... | 2007 | 17202278 |
| autophagy limits listeria monocytogenes intracellular growth in the early phase of primary infection. | autophagy has been recently proposed to be a component of the innate cellular immune response against several types of intracellular microorganisms. however, other intracellular bacteria including listeria monocytogenes have been thought to evade the autophagic cellular surveillance. here, we show that cellular infection by l. monocytogenes induces an autophagic response, which inhibits the growth of both the wild-type and a deltaacta mutant strain, impaired in cell-to-cell spreading. the onset ... | 2007 | 17204850 |
| antimicrobial resistance of listeria spp. recovered from processed bison. | the current study examined the antimicrobial susceptibility of 86 listeria spp. isolated from processed bison carcasses. materials and methods: susceptibility to 25 antimicrobial agents was determined using e-test and national antimicrobial resistance monitoring system (narms) panels. most listeria isolates (88-98%) exhibited resistance to bacitracin, oxacillin, cefotaxime, and fosfomycin. resistance to tetracycline (18.6%) was also common. of the 16 tetracycline-resistant listeria isolates, 15 ... | 2007 | 17209820 |
| endocytic delivery of vancomycin mediated by a synthetic cell surface receptor: rescue of bacterially infected mammalian cells and tissue targeting in vivo. | 2007 | 17212394 | |
| the spectrum of antimicrobial activity of the bacteriocin subtilosin a. | bacterocins are antimicrobial peptides produced by bacteria with a relatively narrow range of activity against closely related organisms. subtilosin a is a bacteriocin produced by bacillus subtilis that has activity against listeria monocytogenes, which might indicate antimicrobial activity unusual for bacteriocins. | 2007 | 17213266 |
| listeria monocytogenes mutants carrying newcastle disease virus f gene fused to its acta and plcb: in vitro expression and immunogenicity in chickens. | recombinant listeria monocytogenes mutants carrying newcastle disease virus (ndv) fusion protein gene f were constructed by homologous recombination. ndv f or its truncated fragment fa was used as the model heterologous gene to be integrated into acta or plcb downstream of their signal sequences. correct orientation of the inserted genes was verified by polymerase chain reaction amplification of f or fa. the inserted f and fa were expressed in the two recombinants lm-deltaacta-f and lm-deltaplcb ... | 2007 | 17213959 |
| multi-virulence-locus sequence typing identifies single nucleotide polymorphisms which differentiate epidemic clones and outbreak strains of listeria monocytogenes. | a recently developed multi-virulence-locus sequence typing (mvlst) method showed improved discriminatory power for subtyping genetically diverse listeria monocytogenes isolates and identified epidemic clone ii isolates associated with two recent u.s. multistate listeriosis outbreaks. to evaluate the ability of mvlst to distinguish other epidemic clones and outbreak strains of l. monocytogenes, 58 outbreak-related isolates from 14 outbreaks and 49 unrelated isolates were analyzed. results showed ... | 2007 | 17215339 |
| a critical role for peptidoglycan n-deacetylation in listeria evasion from the host innate immune system. | listeria monocytogenes is a human intracellular pathogen that is able to survive in the gastrointestinal environment and replicate in macrophages, thus bypassing the early innate immune defenses. peptidoglycan (pg) is an essential component of the bacterial cell wall readily exposed to the host and, thus, an important target for the innate immune system. characterization of the pg from l. monocytogenes demonstrated deacetylation of n-acetylglucosamine residues. we identified a pg n-deacetylase g ... | 2007 | 17215377 |
| identification of ispc, an 86-kilodalton protein target of humoral immune response to infection with listeria monocytogenes serotype 4b, as a novel surface autolysin. | we identified and biochemically characterized a novel surface-localized autolysin from listeria monocytogenes serotype 4b, an 86-kda protein consisting of 774 amino acids and known from our previous studies as the target (designated ispc) of the humoral immune response to listerial infection. recombinant ispc, expressed in escherichia coli, was purified and used to raise specific rabbit polyclonal antibodies for protein characterization. the native ispc was detected in all growth phases at a rel ... | 2007 | 17172332 |
| sensitive listeria spp. immunoassay based on europium(iii) nanoparticulate labels using time-resolved fluorescence. | listeria spp. are gram-positive rod shaped bacteria found universally in the environment. pathogenic listeria monocytogenes is seldom harmful to healthy adults, but can cause serious disease, listeriosis, especially to pregnant women, neonates, and elderly or immunocompromised people. conventional methods for screening listeria in food samples are time consuming and laborious, involving the use of a range of liquid media and plate cultures. in the current study, the total analysis time was short ... | 2007 | 17173997 |
| the effects of growth temperature and growth phase on the inactivation of listeria monocytogenes in whole milk subject to high pressure processing. | the aim of this study was to explore the effect of a wide range of growth temperatures, growth phases and plating media on the inactivation of listeria monocytogenes by high pressure processing (hpp). in part one, l. monocytogenes was grown to mid-stationary phase at 4, 15, 25, 35 or 43 degrees c, inoculated into whole uht milk at approximately 10(7) cfu/ml and high pressure processed at 400 mpa at room temperature (20-25 degrees c). afterward, the hpp milk was plated on tryptic soy yeast extrac ... | 2007 | 17173999 |
| development and application of multiple-locus variable number of tandem repeat analysis (mlva) to subtype a collection of listeria monocytogenes. | in this study we report the development and application of a multiple-locus variable number of tandem repeat analysis (mlva) strategy for subtyping listeria monocytogenes. genome profiles of a collection of forty-five food-borne l. monocytogenes isolates were compared using mlva. these isolates were obtained as part of an active surveillance programme of foods in the south-east region of ireland. mlva successfully discriminated amongst the isolates. the method was easy to perform, relatively fas ... | 2007 | 17174430 |
| factors associated with listeria monocytogenes contamination of cold-smoked pork products produced in latvia and lithuania. | a total of 312 samples of sliced, vacuum packaged, cold-smoked pork from 15 meat processing plants in latvia and lithuania, obtained over a 15-month period from 2003 until 2004, were analyzed for the presence of listeria monocytogenes at the end of their shelf-life. overall, 120 samples (38%) tested positive for l. monocytogenes. despite the long storing period, the levels of l. monocytogenes in cold-smoked pork products were low. manufacturing processes were studied at seven meat processing pla ... | 2007 | 17174432 |
| water activity of bacterial suspension media unable to account for the baroprotective effect of solute concentration on the inactivation of listeria monocytogenes by high hydrostatic pressure. | inactivation of listeria monocytogenes (10(8) cfu/ml) by high hydrostatic pressure (hhp) from 400 to 600 mpa at 25 degrees c for 10 min was investigated with various concentrations of sodium chloride, sucrose, and sodium phosphate buffer solutions. sodium chloride significantly inhibited hhp-induced inactivation of l. monocytogenes at concentrations higher than 2.6 m. a low concentration of sodium chloride within 1.7 m had no effect on hhp-induced inactivation. almost complete inactivation at re ... | 2007 | 17196694 |
| bacteriocin activity of enterococci from rabbits. | the antimicrobial spectrum of bacteriocin-producing enterococcal isolates from rabbits faeces, the biochemical characterization of bacteriocins, and their molecular mass and the presence of structural genes for bacteriocin production were investigated. among enterococci selected from rabbit faeces, six strains of enterococcus faecium (ef2019, ef1819, ef2119, ef1839, ef529, ef24/10) showed inhibitory activity against the indicators e. avium ea5, listeria innocua lmg13568 and listeria monocytogene ... | 2007 | 17216320 |
| effect of heat and high-pressure treatments on microbiological quality and immunoglobulin g stability of caprine colostrum. | caprine colostrums (6 batches) were subjected to heat (56 degrees c for 60 min and 63 degrees c for 30 min) and high-pressure (400 and 500 mpa for 10 min at 20 degrees c) treatments at laboratory scale, and analyses of the main microbial groups and the extent of igg denaturation (determined by immunodiffusion) were performed. overall mean microbial values in raw colostrums were: total count, 5.55 log cfu/ml; enterobacteriaceae, 2.64 log cfu/ml; lactococci, 5.41 log cfu/ml; lactobacilli, 2.34 log ... | 2007 | 17235160 |
| expression and function of synaptotagmin vii in ctls. | the ca(2+) sensor synaptotagmin (syt) vii regulates the exocytosis of conventional lysosomes in several cell types. in ctls, the ca(2+)-regulated exocytosis of lytic granules/secretory lysosomes is responsible for the perforin/granzyme-mediated lysis of target cells. to investigate the role of syt vii in ctl effector function, the expression and function of syt vii were examined in wild-type and syt vii-deficient mice. in comparison with syt vii(+/+) controls, syt vii(-/-) animals were impaired ... | 2007 | 17237398 |
| suppression of the bacterial antigen-specific t cell response and the dendritic cell migration to the lymph nodes by osteopontin. | osteopontin (opn) has been reported to enhance the interferon (ifn)-gamma-producing th1-type t cell response through the induction of interleukin (il)-12 and the suppression of il-10. we therefore investigated whether opn could enhance th1 induction by vaccination against bacterial antigen in vivo. unexpectedly, the co-inoculation of opn suppressed the induction of ifn-gamma-producing cd4(+) t cells and t cell proliferative response after the subcutaneous heat-killed listeria monocytogenes(hklm) ... | 2007 | 17237609 |
| a novel surface autolysin of listeria monocytogenes serotype 4b, ispc, contains a 23-residue n-terminal signal peptide being processed in e. coli. | the 86-kda protein ispc of 774 amino acids in listeria monocytogenes serotype 4b has been recently identified as the target of humoral immune response to listerial infection and as a novel surface autolysin. a signal peptide is predicted at the n-terminal end of ispc, but no biochemical data has been shown to confirm the presence of the cleavage site of a signal peptidase. to address this and prepare sufficient amount of the protein for biochemical and structural characterization, we present a s ... | 2007 | 17239349 |
| fcgammariii-dependent inhibition of interferon-gamma responses mediates suppressive effects of intravenous immune globulin. | intravenous immune globulin (ivig) suppresses autoantibody-mediated inflammation by inducing and activating the inhibitory fc receptor fcgammariib and downstream negative signaling pathways. we investigated the effects of ivig on cellular responses to interferon-gamma (ifn-gamma), a potent macrophage activator that exacerbates inflammation. our study showed that ivig blocked ifn-gamma signaling and ifn-gamma-induced gene expression and suppressed ifn-gamma function in vivo during immune response ... | 2007 | 17239631 |
| exploring the performance of logistic regression model types on growth/no growth data of listeria monocytogenes. | several model types have already been developed to describe the boundary between growth and no growth conditions. in this article two types were thoroughly studied and compared, namely (i) the ordinary (linear) logistic regression model, i.e., with a polynomial on the right-hand side of the model equation (type i) and (ii) the (nonlinear) logistic regression model derived from a square root-type kinetic model (type ii). the examination was carried out on the basis of the data described in vermeu ... | 2007 | 17239980 |
| upregulation of vascular endothelial growth factor by heat-killed listeria monocytogenes in macrophages. | we investigated the effect of heat-killed listeria monocytogenes (hklm) on the expression of vascular endothelial growth factor (vegf) in raw264.7 macrophage-like cells. the expression of vegf was induced in raw264.7 cells treated with hklm. pretreatment of cells with cycloheximide, a protein synthesis inhibitor, inhibited the induction of vegf mrna by hklm. induction of vegf by hklm was partially inhibited by treatment of cells with sb203580, a p38 mitogen-activated protein kinase (mapk) inhibi ... | 2007 | 17240354 |
| genotypic and phenotypic diversity of lactococcus lactis isolates from batzos, a greek pdo raw goat milk cheese. | the genotypic and phenotypic variability of 40 lactococcus lactis isolates obtained from three cheese-making trials of batzos cheese made one in each, winter, spring and summer was investigated. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. results showed a high degree of heterogeneity among strains. according to pfge data, all strains except one were clustered together (at a similarity level of approximately 50%) with t ... | 2007 | 17241681 |
| a pair of 9s: it's in the cards. | new work shows that card9, the recently identified member of the caspase recruitment domain-containing family, directly interacts with the cytoplasmic protein nod2 in the detection of intracellular bacteria. | 2007 | 17242682 |
| reduction in the incidence of invasive listeriosis in foodborne diseases active surveillance network sites, 1996-2003. | listeriosis is a leading cause of death among patients with foodborne diseases in the united states. monitoring disease incidence is an important element of listeriosis surveillance and control. | 2007 | 17243053 |
| listeria monocytogenes infection from foods prepared in a commercial establishment: a case-control study of potential sources of sporadic illness in the united states. | listeria monocytogenes has been estimated to cause >2500 illnesses and 500 deaths annually in the united states. efforts to reduce foodborne listeriosis have focused on foods frequently implicated in outbreaks. potential sources for l. monocytogenes infection not associated with outbreaks remain poorly understood. | 2007 | 17243054 |
| what can we do to prevent listeriosis in 2006? | 2007 | 17243055 | |
| cytosolic expression of seca2 is a prerequisite for long-term protective immunity. | induction of efficient adaptive t cell-mediated immunity against the intracellular bacterium listeria monocytogenes requires its successful invasion of host cell cytosol. however, it is not clear whether its cytosolic escape and growth are sufficient to induce t cell-mediated clearance and protection upon secondary infection. to investigate this issue, we have searched for mutants that do not induce long-term protective immunity yet invade the cytosol of infected cells. we found that mice immuni ... | 2007 | 17244189 |
| differential susceptibility of bone marrow-derived dendritic cells and macrophages to productive infection with listeria monocytogenes. | dendritic cells (dc) are required for the immune response against listeria monocytogenes and are permissive for infection in vivo and in vitro. however, it is unclear if dc provide a desirable intracellular niche for bacterial growth. to address this issue, we have compared the behaviour of l. monocytogenes in murine bone marrow-derived dc and macrophages (bmm). similar to bmm, bacteria escaped to the cytosol in dc, replicated, and spread to adjacent cells. however, dc infection was less robust ... | 2007 | 17250592 |
| carriage of four bacterial pathogens by beef cattle in northern ireland at time of slaughter. | to determine the prevalence of four bacterial zoonotic pathogens in beef cattle at time of slaughter in northern ireland (ni), in order to assess their potential for reducing beef safety. | 2007 | 17257247 |
| inhibition of listeria monocytogenes by freeze-dried piscicocin cs526 fermentate in food. | the effectiveness of freeze-dried powder, fermented with bacteriocin producing carnobacterium piscicola cs526, was evaluated for the inhibition of listeria monocytogenes in a food model. | 2007 | 17257251 |
| limitations in the use of drosophila melanogaster as a model host for gram-positive bacterial infection. | to examine sensitivities of various drosophila melanogaster strains towards human pathogenic and nonpathogenic gram-positive bacteria. | 2007 | 17257264 |
| a molecular beacon-based real time nasba assay for detection of listeria monocytogenes in food products: role of target mrna secondary structure on nasba design. | a molecular beacon-based real-time nasba (qnasba) assay for detection and identification of listeria monocytogenes has been developed. a correlation between targeting highly accessible mrna sequences and qnasba efficiency and sensitivity was demonstrated. the assay targets a sequence from the mrna transcript of the hly gene which is specific for this bacterium; and includes an internal amplification control to disclose failure of the reaction. it was fully selective and consistently detected dow ... | 2007 | 17258831 |
| identification of new noncoding rnas in listeria monocytogenes and prediction of mrna targets. | to identify noncoding rnas (ncrnas) in the pathogenic bacterium listeria monocytogenes, we analyzed the intergenic regions (igrs) of strain egd-e by in silico-based approaches. among the twelve ncrnas found, nine are novel and specific to the listeria genus, and two of these ncrnas are expressed in a growth-dependent manner. three of the ncrnas are transcribed in opposite direction to overlapping open reading frames (orfs), suggesting that they act as antisense on the corresponding mrnas. the ot ... | 2007 | 17259222 |
| piezotolerant small-colony variants with increased thermotolerance, antibiotic susceptibility, and low invasiveness in a clonal staphylococcus aureus population. | following a pressure treatment of a clonal staphylococcus aureus culture with 400 mpa for 30 min, piezotolerant variants were isolated. among 21 randomly selected survivors, 9 were piezotolerant and all formed small colonies on several agar media. the majority of the isolates showed increased thermotolerance, impaired growth, and reduced antibiotic resistance compared to the wild type. however, several nonpiezotolerant isolates also demonstrated impaired growth and the small-colony phenotype. in ... | 2007 | 17259364 |
| ischemic cerebral attacks due to a pseudo-aneurysm of the internal carotid artery with listeria monocytogenes. | 2007 | 17260177 | |
| in vitro and in vivo activities of pd 0305970 and pd 0326448, new bacterial gyrase/topoisomerase inhibitors with potent antibacterial activities versus multidrug-resistant gram-positive and fastidious organism groups. | pd 0305970 and pd 0326448 are new bacterial gyrase and topoisomerase inhibitors (quinazoline-2,4-diones) that possess outstanding in vitro and in vivo activities against a wide spectrum of bacterial species including quinolone- and multidrug-resistant gram-positive and fastidious organism groups. the respective mics (microg/ml) for pd 0305970 capable of inhibiting>or=90% of bacterial strains tested ranged from 0.125 to 0.5 versus staphylococci, 0.03 to 0.06 versus streptococci, 0.25 to 2 versus ... | 2007 | 17261623 |
| evaluation of housekeeping genes in listeria monocytogenes as potential internal control references for normalizing mrna expression levels in stress adaptation models using real-time pcr. | listeria monocytogenes is an important food-borne pathogen that can tolerate a wide range of stress conditions. however, its stress adaptation processes are still poorly understood. real-time-based quantitative rt-pcr (qrt-pcr) provides a tool to probe gene expression changes underlying stress adaptation. but, a limitation to study mrna levels by real-time qrt-pcr is that validated reference genes are required for normalization. such genes are currently lacking for experimental models that may b ... | 2007 | 17263845 |
| traditional dry fermented sausages produced in small-scale processing units in mediterranean countries and slovakia. 1: microbial ecosystems of processing environments. | microbial ecosystems were surveyed in 314 environmental samples from 54 southern and eastern european small-scale processing units (pus) manufacturing traditional dry fermented sausages. the residual microflora contaminating the surfaces and the equipment were analysed after cleaning and disinfection procedures. all the pu environments were colonised at various levels by spoilage and technological microflora with excessive contamination levels in some of the pus. sporadic contamination by pathog ... | 2007 | 22061943 |
| listeria monocytogenes: occurrence in beef and identification of the main contamination points in processing plants. | this study aimed to establish the occurrence of listeria spp., especially l. monocytogenes and its main serotypes, in beef and processing plants. a total of 443 samples were obtained from equipment, installations and products from 11 meat processing establishments from paraná state, brazil. all samples were analyzed using usda methodology for listeria spp. detection, followed by species identification. the occurrence of listeria spp. in the samples was 38.1% of which 51.4% were from equipment, 3 ... | 2007 | 22061233 |
| the fate of two listeria monocytogenes serotypes in "cig kofte" at different storage temperatures. | cig kofte is a traditional turkish food prepared from minced beef, bulgur, onions, garlic and varieties of spices. it is generally consumed within a few hours. however, leftovers can be kept in refrigerator or in room temperature up to 24h until they are consumed. in this study, survival and growth of two listeria monocytogenes serotypes were investigated in cig kofte during the storage. for this purpose, the prepared samples were separately contaminated with serotypes 1/2b or 4b of l. monocytog ... | 2007 | 22064198 |
| influence of oxygen exclusion and temperature on pathogenic bacteria levels and sensory characteristics of packed ostrich steaks throughout refrigerated storage. | ostrich steaks (290) were obtained from iliofibularis muscles. for microbiological and ph determinations, samples were inoculated with listeria monocytogenes nctc 11994 (80 steaks) or escherichia coli atcc 12806 (80), then air- or vacuum-packed and stored at either 4±1°c or 10±1°c. analyses were carried out on days 0, 3, 6 and 9 of storage. for sensory evaluation, samples (130) were air- or vacuum-packed and stored at 4±1°c or at 10±1°c. sensory attributes (odour, colour, drip loss, texture and ... | 2007 | 22064287 |
| diversity of microorganisms in the environment and dry fermented sausages of small traditional french processing units. | naturally fermented sausages produced in nine traditional french processing units and their environmental surfaces were characterised by microbial and physico-chemical analyses. salmonella and staphylococcus aureus were not detected in the environment whereas listeria monocytogenes was detected in four samples. staphylococcus/kocuria, lactic acid bacteria (lab), enterobacteriaceae, pseudomonas, yeasts/moulds and enterococci contaminated the surfaces of two processing units, indicating insufficie ... | 2007 | 22064197 |
| the effect of nisin on l. monocytogenes in turkish fermented sausages (sucuks). | turkish fermented sausage (sucuk) is a traditional, well-known meat product in turkey. the aim of this study was to evaluate the effect of different nisin concentrations on listeria monocytogenes in experimentally contaminated sucuk. analyses were performed on at 0, 1, 3, 5, 7, 10, 15, 20, 25 and 30days for l. monocytogenes and other microbiological parameters (total mesophilic aerobic bacteria and lactic acid bacteria) and physico-chemical parameters (ph, a(w) and moisture content).the sucuk do ... | 2007 | 22064303 |
| antilisterial activity of lactic acid bacteria isolated from "alheiras" (traditional portuguese fermented sausages): in situ assays. | a total of 226 lactic acid bacteria (lab) isolated from "alheira", a traditional portuguese fermented sausage, were screened for antagonistic activity against some pathogenic microorganisms, including listeria monocytogenes. the objective was to isolate lab with antibacterial activity from "alheiras" and to select strains that could be used in "alheira" production. isolates displaying antibacterial activity against listeria innocua and l. monocytogenes were investigated for the nature of the ant ... | 2007 | 22061260 |
| antimicrobial activity of various cationic molecules on foodborne pathogens. | antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, gram-positive and gram-negative bacteria. the minimum inhibitory concentration (mic) and the minimum bactericidal concentration (mbc) of α-poly-l-lysine (poly-lys), α-poly-l-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on bacillus subtilis, ... | 2007 | 27517822 |
| [impact of infection with listeria monocytogenes on the pregnancy]. | during a ten years period (06.1998a.-06.2007) at the delivery ward of maychin dom university hospital were admitted 31 627 parturients. among all of them we indentified and evaluated only 6 pregnant patients with listeria monocytogenes infection. during the first 8 years of the investigated period there was no even single case of the infection, whilst during the last 2 years the frequency of the listeria infection among all birth deliveries was 0.10%. the first five of our cases were admitted wi ... | 2007 | 19705692 |
| simultaneous detection of escherichia coli o157:h7, salmonella spp. and listeria monocytogenes with an array-based immunosorbent assay using universal protein g-liposomal nanovesicles. | a novel universal reagent for immunoassays, protein g-liposomal nanovesicles has been developed and successfully used in an immunomagnetic bead sandwich assay for the detection of escherichia coli o157:h7 [c.-s. chen, a.j. baeumner, r.a. durst, talanta 67 (2005) 205]. to demonstrate the universal capability of protein g-liposomal nanovesicles, this reagent was used to develop an array-based immunosorbent assay for the simultaneous detection of e. coli o157:h7, salmonella, and listeria monocytoge ... | 2006 | 18970559 |
| "mini-array" transcriptional analysis of the listeria monocytogenes lecithinase operon as a class project: a student investigative molecular biology laboratory experience*. | this report describes a molecular biotechnology-based laboratory curriculum developed to accompany an undergraduate genetics course. during the course of a semester, students researched the pathogen, developed a research question, designed experiments, and performed transcriptional analysis of a set of genes that confer virulence to the food-borne pathogen, listeria monocytogenes. gene fragments were amplified via pcr and utilized in "mini-arrays," a dot-blot-based format suitable for the simult ... | 2006 | 21638678 |
| role of quantitative risk assessment and food safety objectives in managing listeria monocytogenes on ready-to-eat meats. | listeria monocytogenes may be found on ready-to-eat (rte) meats, posing a public health risk. to minimize the public health impact, an appropriate level of protection (alop) can be established for a population with respect to l. monocytogenes, and ideally should be based on a scientific assessment of the risk, as well as societal and economic factors. food safety systems can be based on meeting the alop. food safety objectives (fso) provide a link between the alop and performance objectives that ... | 2006 | 22062717 |
| prevalence of listeria species in camel sausages from retail markets in aydin province in turkey and rapd analysis of listeria monocytogenes isolates. | samples were taken from 100 camel sausages from the different retail markets in aydin province in the south-west of turkey and they were tested for the presence of listeria spp by biochemical methods. samples were enriched using listeria enrichment broth and they were inoculated onto listeria selective agar. listeria monocytogenes was isolated from nine samples (9%), listeria innocua from 14 samples (14%) and listeria welshimeri from two samples(2%). a 701 bp fragment of listeriolysin o sequence ... | 2006 | 21851683 |
| microbial heat resistance of listeria monocytogenes and the impact on ready-to-eat meat quality after post-package pasteurization. | several methods using bactericides, hydrostatic pressure, and post-package pasteurization technologies to control listeria monocytogenes (lm) in ready-to-eat meats have been attempted. in addition to controlling lm contamination, any newly developed technology must have minimal effects on organoleptic properties. the objectives of this study were to: (1) determine the heat resistance of lm in two brands (a and b) of bologna differing in formulations, and, (2) evaluate the effects of post-package ... | 2006 | 22063046 |
| antilisterial activity of lactic acid bacteria inoculated on cooked ham. | this study was conducted to evaluate the ability of lactobacillus sakei 1, a bacteriocin-producing (bac(+)) lactic acid bacterium (lab), isolated from brazilian fresh pork sausage to inhibit two listeria monocytogenes strains (serotypes 4b and 1/2a) on cooked, sliced vacuum-packaged ham. l. sakei atcc 15521 was used as a non-bacteriocin producer (bac(-)). l. monocytogenes (ca. 2 logcfu/ml) and lab (ca. 6 logcfu/ml) were inoculated on the sterilized ham, vacuum-sealed and incubated at 8°c for 10 ... | 2006 | 22063215 |
| effect of a bacteriocin produced by pediococcus acidilactici against listeria monocytogenes and clostridium perfringens on spanish raw meat. | the inhibitory effect of a bacteriocin, produced by pediococcus acidilactici, against listeria monocytogenes and clostridium perfringens on spanish raw meat surface, was evaluated by in situ assays. samples were incubated with the bacteriocin and then with a culture of the pathogenic bacteria. the treatment with 500, 1000 or 5000 bacteriocin units/ml (bu/ml) reduced the counts of l. monocytogenes after storage at 15°c during 72h by 1, 2 or 3 log cycles and with 1000 or 5000 bu/ml after storage a ... | 2006 | 22061374 |
| a note on the incidences of salmonella spp., listeria spp. and escherichia coli o157:h7 serotypes in turkish sausage (soudjouck). | the incidence of salmonella spp., listeria monocytogenes and escherichia coli o157:h7 was determined in 100 turkish sausage (soudjouck) samples collected from shops and markets in the afyon province, turkey. salmonella spp. were detected in 7% of the samples. all of the isolates were s. enterica paratyphi b. in addition, listeria spp. were detected in 9% of the samples. its distribution was 7% l. monocytogenes and 1% each of l. ivanovii and l. innocua. serological study of the seven l. monocytog ... | 2006 | 22061388 |
| influence of rosemary-tocopherol/packaging combination on meat quality and the survival of pathogens in restructured irradiated pork loins. | irradiated restructured pork loins treated with rosemary-tocopherol/double-packaging had lower tbars values than vacuum-packaged control after 10 days of refrigerated storage. the rosemary-tocopherol combination, however, had no effect on the production of sulfur volatiles responsible for the irradiation off-odor, and color changes in irradiated pork. v7/a3 double-packaging was effective in reducing the sulfur volatiles significantly. rosemary-tocopherol combination was highly effective in reduc ... | 2006 | 22062849 |
| the sensory acceptability of cooked meat products treated with a protective culture depends on glucose content and buffering capacity: a case study with lactobacillus sakei 10a. | biopreservation has been proven to be a promising natural preservation technique, but the impact of protective cultures on the sensory properties of cooked meat products (cmp) is not well documented. this work presents a case study on the protective culture lactobacillus sakei 10a to obtain a clear view on the real consequences of using protective cultures on the sensory quality of cmp. a preliminary screening study on 13 different cmp and more elaborate application trials at 7°c on vacuum packa ... | 2006 | 22063058 |
| host resistance model to an intracellular pathogen. | this unit describes a model for the determination of host resistance in mice using a gram-positive intracellular bacterium, listeria monocytogenes (lm), and discusses its broad use in immunotoxicological studies. the provides detailed procedures for lm infection and sample analysis, including preparation and infection of mice with lm, recording of sickness behaviors in infected mice, and determination of viable lm numbers in the tissues from infected animals. the protocol also describes use of s ... | 2006 | 23045129 |
| [a fatal case of listeria monocytogenes sepsis and meningitis in a patient with wegener's granulomatosis]. | the bacillus listeria monocytogenes is widely distributed in the environment. listeria monocytogenes most often causes infection in the neonates, pregnant women, elderly and immunosuppressed persons. we report on a case of fatal sepsis and meningitis in a 59-year-old woman receiving cyclophosphamide and glucocorticoid therapy for wegener's granulomatosis over a 10-year period. listeriosis should be suspected in case of sepsis and/or meningitis in patients who receive immunosuppressive agents. si ... | 2006 | 17217109 |