Publications

functional and nmr studies of hb sassari (asp-126 alpha----his); role of the inter-subunit contacts in the affinity control of human hemoglobin.	the oxygen affinity of hemoglobin sassari (asp-126 alpha----his), a variant substituted in the alpha 1 beta 1 interface, was found to be 8-times greater relative to normal adult human hemoglobin. study of the exchangeable hydrogen-bonded protons by nmr spectroscopy shows only minor changes at the alpha 1 beta 1 interface. in particular, the resonance previously assigned to the proton of the hydrogen bond asp-126 alpha 1. . . tyr-35 beta 1 in normal hemoglobin is still present in the variant spec ...	1990	2268670
survey of enteropathogenic agents in children with and without diarrhoea in ghana.	a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ...	1990	2270006
effects of tokishakuyakusan and keishibukuryogan on steroidogenesis by rat preovulatory follicles in vivo.	the effect of tokishakuyakusan (ts) and keishibukuryogan (kb) on estradiol-17 beta, progesterone and testosterone in serum and ovarian tissue from pms-treated immature rats was examined in vivo. the in vivo intravenous administration of 20 micrograms of ts or kb increased the concentrations of estradiol-17 beta, progesterone and testosterone. these results suggest that ts and kb stimulate in vivo the production of estradiol-17 beta, progesterone and testosterone by preovulatory follicles, and th ...	1990	2270849
induction of g.c to a.t transitions by the acridine half-mustard icr-191 supports a mispairing mechanism for mutagenesis by some bulky mutagens.	as the most nucleophilic atom in dna, the guanine n7 atom is a major site of attack for a large number of chemical mutagens as well as chemotherapeutic agents. paradoxically, while methylation of guanine n7 is believed to be largely nonmutagenic, aflatoxin b1, among the most potent mutagens, appears to exert its mutagenic activity through adduction at this site. on the basis of an analysis of the specificity of mutations induced by various adduct forms of aflatoxin b1, we have previously propose ...	1990	2271689
toxic dilatation of the colon in shigellosis.		1990	2271862
shigellosis in new brunswick.		1990	2272103
dna sequence analysis of the reca genes from proteus vulgaris, erwinia carotovora, shigella flexneri and escherichia coli b/r.	the complete nucleotide sequences of the reca genes from escherichia coli b/r, shigella flexneri, erwinia carotovora and proteus vulgaris were determined. the dna sequence of the coding region of the e. coli b/r gene contained a single nucleotide change compared with the e. coli k12 gene sequence whereas the s. flexneri gene differed at 7 residues. in both cases, the predicted proteins were identical in primary structure to the e. coli k12 reca protein. the dna sequences of the reca genes from e ...	1990	2274037
[role of protons and bicarbonate in adaptative gastric cytoprotection].	the unknown mechanism of adaptative gastric cytoprotection (agc) induced by 20% ethanol and subsequent injury with 70% ethanol was studied in wistar rats. pretreatment with indomethacin or hgii2 did not prevent the agc, there suggesting that neither endogenous pgs nor gastric mucus take part in its mechanism. on the other hand, ranitidine pretreatment blocked and even aggravated the damage induced by ethanol-ethanol. in contrast, the latter phenomenon was reverted by 20% acidified ethanol. it is ...	1990	2275313
protective milk o antibodies induced in guinea pigs by parenteral shigella ribosomal vaccine.	igg and iga o antibodies were studied in milk and sera of guinea pigs subcutaneously immunized at various stages of pregnancy with shigella ribosomal vaccines (srv) from shigella sonnei and shigella flexneri. both vaccines induced o antibodies in milk, the level of iga antibodies being significantly higher than that of igg antibodies. the immune milk provided a clear-cut protection against experimental shigella-induced keratoconjunctivitis. these results are consistent with the previously shown ...	1990	2276843
bone allografts sterilised by irradiation. biological properties, procurement and results of 150 massive allografts.	massive bone allografts from cadavers have been sterilised by gamma-radiation from radioactive cobalt at a dose of 25,000 gray (gy). the biological effects of radiation are discussed. human cortical bone showed an acceptable 20% decrease in strength on bending tests after 27,000 gy irradiation, but higher doses are more damaging and should be avoided. the procurement protocol used at the cochin hospital is described, and the importance of dosimetry and record-keeping emphasised. the clinical res ...	1990	2279829
[current views on the pathomorphology and pathogenesis of dysentery].	according to the literature and the authors' data in patients who died of dysentery shigellae are found seldom because of postmortem shedding of superficial colonic epithelium infected by them. shigella adhesion and invasion into the colonocytes are regularly found in the colon biopsies. as shown recently in experiments, shigella outer membrane proteins forming "contact haemolysin" ("virulence plasmid" product) are responsible for their invasion. in the small intestine this cytotoxin is destroye ...	1990	2281980
[the characteristics of the proliferative processes in the epithelium of the sigmoid intestine in acute bacillary dysentery].	proliferative processes were studied using radioautography with 3h-thymidine at the height of the disease and in the early convalescence of the bacterial dysentery. the mucous membrane of the sigmoid colon of healthy persons undergoing rectoromanoscopy due to the epidemic reasons served as control. no pathologic changes were found in these healthy persons; the labeled nuclei index (lni) was 5.75 +/- 0.4%, the label intensity (li) characterizing the rate of dna synthesis was 14.9 +/- 0.8. during ...	1990	2285363
[the epidemiological characteristics of shigellosis].		1990	2286252
antigenic and catalytic disparity in the distribution of cytochrome p-450-dependent 25-hydroxyvitamin d3-1 alpha- and 24-hydroxylases.	chick 25-hydroxyvitamin d3-1 alpha-hydroxylase, a cytochrome p-450 monooxygenase with a molecular weight of 57 kda, can be isolated as described by mandel et al. (1990 b). under normal physiological circumstances, it occurs exclusively in kidney mitochondria. an isozyme of the 1 alpha-hydroxylase, known as the 24-hydroxylase, which uses the same substrate to yield an isomeric product, is also a cytochrome p-450 monooxygenase, has a molecular weight of 55 kda, and like-wise occurs in kidney mitoc ...	1990	2286531
[the differential diagnosis of intestinal amebiasis].	differential diagnosis of intestinal amebiasis (106 patients), acute flexner's dysentery (105 patients) and nonspecific ulcerative colitis (31 patients) based on the clinical data and rectoromanoscopic findings is described. rapidly and gradually progressing in the first 7 days intestinal amebiasis is characterized by a gradual onset, normal t0 with mounting general weakness, celiectasia, diarrhea with hyaloid mucus, focal catarrh-hemorrhagic changes and appearance of individual oval transversal ...	1990	2290391
association of thrombotic thrombocytopenic purpura and human immunodeficiency virus infection.	a 35 year-old hiv-positive male intravenous drug abuser developed thrombotic thrombocytopenic purpura (ttp) during the course of recto-sigmoiditis secondary to shigella flexneri. complete remission was achieved by aggressive treatment of a combination of plasma exchange, fresh frozen plasma infusion, continuous prostacyclin perfusion, p.o. administered aspirin-dipyridamol and intravenous injections of vincristine. during acute microangiopathy, an acquired type ii von willebrand disease was diagn ...	1990	2290713
intracellular lytic enzyme systems and their use for disruption of escherichia coli.	this article focusses on lytic enzyme systems available in e. coli and their potential use for cellular disruption. in the systems described here the genetic information for lysis would be carried within the microbial host, either integrated or naturally occurring on chromosomal dna, or on extrachromosomal elements such as plasmids. each microbe would carry complete information for endogenous enzymatic lysis, and lysis would occur in a controlled manner after being triggered by an external facto ...	1990	2291440
changes in the antibiotic sensitivities of urinary pathogens, 1971-1989.	all urinary pathogens from general practice and hospital have been tested for sensitivity to a range of antimicrobial agents for the last 19 years. there have been marked changes during that time. in general practice there has been a considerable increase in the proportion of staphylococcal infections from 5.1% to a peak of 14.8% in 1982 and a more recent decline to 3.4%. there has also been a decrease in the proportion caused by proteus mirabilis, from 9.2% to 5.0%. similar, but smaller changes ...	1990	2292543
production of propeptide-directed antibody: its application to the purification of proalbumin and analysis of proalbumin processing.	antibodies which specifically recognize rat proalbumin, but not mature serum-albumin, were raised. propeptide (nh2-arg-gly-val-phe-arg-arg) with an additional cysteine residue at the carboxyl terminus was conjugated to ovalbumin through either sulfide or amino group and the conjugates were injected into rabbits. monospecific antibodies were purified on a propeptide-coupled affinity column and further immobilized as an affinity support, allowing us to purify proalbumin in a single step from rat l ...	1990	2292582
a sensitive in vitro functional assay to detect k(+)-channel-dependent vasodilators.	this study describes a sensitive in vitro relaxation assay using isolated rabbit mesenteric artery to detect the activity of a vasodilator as a k(+)-channel activator. thus, comparison of several known k(+)-channel activators was made with other vasodilators known to work via various cellular mechanisms. the vasodilators used were minoxidil sulfate (mnxs; 5 microm), brl-34915 (cromakalim, 0.1 microm), nicorandil (10 microm), pinacidil (1 microm), diazoxide (100 microm), sodium nitroprusside (10 ...	1990	2292878
evaluation of combined effects in dose-response studies by statistical comparison with additive and independent interactions.	an improved method for the evaluation of combined drug effects by means of dose-response curves (drcs) is described. a drug, a, was tested in the absence and presence of a fixed concentration of another drug, b, mainly in organ-bath experiments with smooth muscle strips from bovine coronary arteries and tracheal muscle. the results of such experiments are expressed in terms of percent of maximum response. median values, rather than mean values, for each concentration of drug a were determined in ...	1990	2292882
in vitro skin absorption and metabolism of benzoic acid, p-aminobenzoic acid, and benzocaine in the hairless guinea pig.	the percutaneous absorption and metabolism of three structurally related compounds, benzoic acid, p-aminobenzoic acid (paba), and ethyl aminobenzoate (benzocaine), were determined in vitro through hairless guinea pig skin. benzocaine was also studied in human skin. absorption of benzocaine was rapid and similar through both viable and nonviable skin. the absorption of the two acidic compounds, benzoic acid and paba, was greater through nonviable skin. a small portion (6.9%) of absorbed benzoic a ...	1990	2293213
release of human serum albumin from poly(lactide-co-glycolide) microspheres.	human serum albumin (hsa) was encapsulated in a 50:50 copolymer of dl-lactide/glycolide in the form of microspheres. these microspheres were used as a model formulation to study the feasibility of controlling the release of large proteins over a 20- to 30-day period. we show that hsa can be successfully incorporated into microspheres and released intact from these microspheres into various buffer systems at 37 degrees c. a continuous release of the protein could be achieved in physiological buff ...	1990	2293220
production of specific monoclonal antibodies to salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever.	four murine monoclonal antibodies (mabs) to salmonella typhi flagellin were produced. these mabs did not react with eight other enterobacterial strains tested: salmonella enteritidis, salmonella typhimurium, salmonella paratyphi a, escherichia coli, shigella flexneri, shigella sonnei, yersinia enterocolitica, and campylobacter jejuni. all four mabs cross-reacted with salmonella muenchen flagellin indicating specificity for d antigenic flagellar epitope. one mab (c4) was selected to develop a dou ...	1990	2295860
investigation by amperometric methods of intracellular reduction of 2,6-dichlorophenolindophenol in normal and transformed hepatocytes in the presence of different inhibitors of cellular metabolism.	the reduction of 2,6-dichlorophenolindophenol (dcip) was measured by amperometric methods in morris hepatoma 3924a cells, normal isolated rat hepatocytes and in mitochondria isolated from normal rat liver. the influence of aerobic and anaerobic atmospheres and of various inhibitors of cellular metabolism, especially of the respiratory chain (kcn, nan3, oligomycin), on dcip-reduction were studied using glucose, succinate, beta-hydroxybutyrate, alpha-keto-glutarate and oxalacetate as substrates. u ...	1990	2297512
virg, an agrobacterium tumefaciens transcriptional activator, initiates translation at a uug codon and is a sequence-specific dna-binding protein.	the agrobacterium tumefaciens ti plasmid virg locus, in conjunction with vira and acetosyringone, activates transcription of the virulence (vir) genes. insertional and deoxyoligonucleotide-directed mutagenesis studies showed that both octopine and nopaline ti plasmid virg genes initiate translation at a uug codon. virg protein initiated at this uug codon was found to be 241 amino acid residues in length and had an apparent molecular mass of 27.1 kilodaltons. a salmonella typhimurium trp-virg tra ...	1990	2307647
usefulness of rapid production model for pancreatic carcinoma in male hamsters.	the rapid production model of pancreatic duct adenocarcinoma established previously in female syrian hamsters was applied to male hamsters to confirm the efficacy of the "augmentation pressure" procedure and to reveal sex difference in response to this procedure. hamsters received 70 mg/kg n-nitrosobis (2-oxopropyl) amine (bop) as an initiation and two cycles of "augmentation pressure" consisting of ethionine on a choline-deficient diet, methionine and bop and an additional 20 mg/kg bop and were ...	1990	2317782
ca2+ transients are not required as signals for long-term neurite outgrowth from cultured sympathetic neurons.	a method for clamping cytosolic free ca2+ ([ca2+]i) in cultures of rat sympathetic neurons at or below resting levels for several days was devised to determine whether ca2+ signals are required for neurite outgrowth from neurons that depend on nerve growth factor (ngf) for their growth and survival. to control [ca2+]i, normal ca2+ influx was eliminated by titration of extracellular ca2+ with egta and reinstated through voltage-sensitive ca2+ channels. the rate of neurite outgrowth and the number ...	1990	2324199
tibenelast, 5,6-diethoxybenzo(b)thiophene-2-carboxylic acid, sodium salt (ly186655), is an orally active anti-asthma compound in the guinea pig.	anaphylactic shock was induced in actively sensitized guinea pigs by free inhalation of a high dose of ovalbumin (10 mg/ml) aerosol. tibenelast (ly186655), 5,6-diethoxybenzo(b)-thiophene-2-carboxylic acid, sodium salt, proved to be a potent orally active compound against anaphylactic shock induced by high dose antigen aerosol. when a lower aerosol challenge (0.05 mg/ml) was employed, bronchoconstriction was observed with a concomitant increase in lung resistance (rl) and a fall in dynamic compli ...	1990	2329918
therapeutic value of gramurin (oxolinic acid) in shigella infections and shigella-negative dysentery syndrome.	a total of 10 shigella-positive and 10 shigella-negative dysentery syndrome patients were treated with daily 2-g gramurin doses for 3 days. favourable clinical results could be obtained in response to the therapy. the complaints of the patients ceased within 48 hours. bacterial negativity was recorded on days 3, 4, and 5 after cessation of therapy in shigella flexneri-positive cases (5 cases), while in case of shigella sonnei positivity bacterium negativity could be obtained within the abovement ...	1990	2345905
epidemiology of bacterial pathogens associated with infectious diarrhea in djibouti.	during a survey examining the causes of diarrhea in the east african country of djibouti, 140 bacterial pathogens were recovered from 209 diarrheal and 100 control stools. the following pathogens were isolated at comparable frequencies from both diarrheal and control stools: enteroadherent escherichia coli (eaec) (10.6 versus 13%), enterotoxigenic e. coli (etec) (11 versus 10%), enteropathogenic e. coli (epec) (7.7 versus 12%), salmonella spp. (2.9 versus 3%), and campylobacter jejuni-c. coli (3 ...	1990	2351738
role of bacterial and host factors in the pathogenesis of shigella septicemia.	to determine the role of bacterial and host factors in the pathogenesis of shigellemia, blood and fecal shigella isolates were compared for serum resistance and siderophore production, and shigellemic patients were examined for decreased serum bactericidal activity or increased serum transferrin saturation compared to control patients with non-bacteremic shigellosis. the majority of both blood (36/38) and fecal (36/48) shigella isolates were sensitive to normal serum (greater than 2 log kill/60 ...	1990	2373088
alterations of cellular characteristics of a human ovarian teratocarcinoma cell line after in vitro treatment with retinoids.	differentiation of the human teratocarcinoma derived cell line. pa-1, with retinoids was examined at concentrations (10(-6)-10(-8) m) that did not exhibit an antiproliferative effect during log-phase growth. treatment with naturally occurring retinoic acid or certain synthetic retinoids (13-cis retinoic acid, ro10-9359, and ro13-7410), while not significantly altering the log-phase growth rate, decreased the saturation cell density and mitotic indices after confluence. retinoid treatment also in ...	1990	2373285
effect of neuromuscular blockade on fetal oxidative metabolism.	because fetal neuromuscular blockade has been used during cord blood sampling procedures to prevent fetal movement, we studied the effect of pancuronium bromide on fetal arterial concentrations of glucose, lactate, and oxygen and their umbilical uptake in 6 chronically prepared fetal sheep. we observed a significant increase in the arterial concentrations and decrease in the umbilical uptakes. we suggest that cord blood sampling procedures for fetal assessment of oxidative metabolism not be perf ...	1990	2390540
morphologic characterization of osteoblast-like cell cultures isolated from newborn rat calvaria.	two methods for harvesting osteoblast-like cell populations from newborn (10 days) rat calvaria were compared. the first one consisted in culturing the periosteum-free bones and then trypsinizing the cells on the bone surface. the second one involved the migration of the osteoblasts on glass fragments before trypsinization. since the plating efficiency, the proportion of alkaline phosphatase-positive cells, the population doubling time, and the calcium deposition were more adequate, the second m ...	1990	2390733
phosphorylation of the virg protein of agrobacterium tumefaciens by the autophosphorylated vira protein: essential role in biological activity of virg.	agrobacterium tumefaciens virulence genes are induced by plant signals through the vira-virg two-component regulatory system. the vira protein is a membrane-spanning sensor molecule that possesses an autophosphorylating activity, and the virg protein is a sequence-specific dna-binding protein. in this report, we demonstrate that the virg protein is phosphorylated by the vira protein and that the phosphate is directly transferred from the phosphorylated vira molecule (phosphohistidine) to the vir ...	1990	2394678
tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners.	the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ...	1990	2394748
onset of the hormone-sensitive perinatal period for sexual differentiation of the sexually dimorphic nucleus of the preoptic area in female rats.	the volume of the sexually dimorphic nucleus of the preoptic area (sdn-poa) of the rat brain is severalfold larger in males than in females. the volume of the sdn-poa can be influenced significantly by the hormonal milieu during the perinatal "critical period" of sexual differentiation of the brain. the purpose of the present study was to determine the onset of this period of sexual differentiation of the sdn-poa. pregnant rats received no treatment or were injected subcutaneously with oil on da ...	1990	2394991
effects of castration and testosterone treatment on the activity of testosterone-metabolizing enzymes in the brain of male and female zebra finches.	recently, we described the distribution of testosterone-metabolizing enzymes (i.e., aromatase, 5 alpha- and 5 beta-reductases) in the zebra finch (taeniopygia guttata) brain using a sensitive radioenzyme assay combined to the palkovits punch method. a number of sex-differences in the activity of these enzymes were observed especially in nuclei of the song-control system. the hormonal controls of these differences have now been analyzed by gonadectomizing birds of both sexes and by giving them a ...	1990	2394994
effect of glycerol on the hemodynamics of acutely induced ischemic area in the cerebral cortex of cats.	the effect of glycerol on the hemodynamics of ischemic areas of the brain was examined. employing a photoelectric apparatus, the cbv (vol%), mean transit time of blood(s), and cbf (ml/100 g/min) in the cortex of the left ectosylvian gyrus were determined in 16 anesthetized cats before, at 1 hr, and at 2 hr after left mca occlusion. in 10 cats, 10% glycerol in 5% fructose saline was intravenously administered at a speed of 5 ml/kg/hr at 1 hr after mca occlusion. the other animals served as the co ...	1990	2396522
doxycycline prophylaxis for shigellosis.	the efficacy of oral doxycycline, 100 mg/d for 14 days, in reducing the incidence of shigellosis in newcomers to an area hyperendemic for the disease was examined in a double-blind, placebo-controlled trial. of 107 entrants, 100 completed the study; 50 received the drug and 50 received a placebo. diarrheal disease and associated symptoms were monitored for 8 weeks. starting on the 3rd day of the trial, an outbreak was observed, and shigella flexneri type 2a was isolated from 6 subjects. eight of ...	1990	2404480
contribution of superoxide dismutase and catalase activities to shigella flexneri pathogenesis.	a shigella flexneri serotype 5 strain deficient in the production of the iron-containing superoxide dismutase fesod (sodb) and a catalase-negative (katfg) s. flexneri serotype 5 strain were isolated. both strains were examined for increased sensitivity to oxygen stress by using assays involving killing by mouse peritoneal macrophages and human polymorphonuclear leukocytes as well as infection of rabbit ileal loops. the sodb mutant was extremely sensitive to killing by phagocytes when compared wi ...	1990	2404874
acute glomerulonephritis due to shigella flexneri dysentery with demonstration of a virulence protein of shigella in circulating immune complexes.		1990	2405072
cloning vectors derived from streptomyces niveus plasmid psn2.	two high copy number, broad host range, general purpose cloning vectors, plg5 and plg10, derived from the unstable streptomyces niveus plasmid psn2 are described. plg5 (5.5 kb) and plg10 (6.5 kb) both carry the thiostrepton resistance (tsrr) and lethal zygosis (ltz+) markers and have single cloning sites within a non-essential region and the tsr gene. plg505 (7.4 kb) was constructed by cloning the viomycin resistance (vph) gene into the single bamhi site of plg5 to give a further vector with ins ...	1990	1369288
[genetic counseling of parents with children with clefts].	the elucidation of the aetiology of the individual case has proved to be of fundamental importance to the counselling interview as well as to the assessment of the risk for further affected children. up to now, a genetic cause in the form of chromosomal or gene mutations can be assumed only if the cleft is related to a distinct syndrome. an isolated cleft obviously owes its origin to a combination of genetic and extrinsic factors. consequently, the assessment of the risk of repetition is to a gr ...	1990	296849
antithrombotic therapy for coronary artery disease and valvular heart disease.		1990	2271788
the early periodic screening, diagnosis, and treatment program [epsdt]: status of progress and implementation in 51 states and territories.		1990	258722
a primer on labor unionism.		1990	258489
studies on the prevalence of renal disease and hypertension in relation to schistosomiasis. iv. systemic blood pressure hypertension and related features.	two comparable rural communities but with varying endemicity of urinary schistosomiasis in environs of ibadan, nigeria, have been studied by a total cross-sectional population survey. it has been found that:- (i) schistosomiasis does not predispose to raised blood pressure (ii) both diastolic and systolic blood pressure increase with age. (iii) prevalence of systemic hypertension is higher in females than males (iv) body weight and height are related to hypertension in both males and females (v) ...	1990	313638
1. home treatment of hemophiliacs in france.		1990	268020
control of growth of benzo(a)pyrene-transformed 3t3 cells.	the growth controls observed in benzo[a]pyrene-transformed 3t3 cells (bp3t3) are compared with those of virus-transformed and normal 3t3 cells. superficially, the chemically transformed bp3t3 cells have the same behavior as virus-transformed sv3t3 cells. both grow to high cell density in culture medium with 10% serum, both form colonies in methocel, and both are tumorigenic. closer examination, however, has disclosed that bp3t3 cells exhibit "normal" growth controls at low serum concentrations. ...	1990	184467
disease: its cause and effect. 6: immunological diseases.		1990	258469
geriatric medicine: imperatives.		1990	267845
periodontal sensory innervation of the dentition of the japanese shrew mole.	the neurohistological investigation of the periodontal sensory innervation in the whole dentition of the japanese shrew mole showed that the periodontium is much more densely supplied by the free nerve endings at the apical region than at the intermediate one at a ratio of about 8:1 in the upper dentition and 5:1 in the lower dentition. the interdental gingival tissue is innervated by the nerve plexus formed by the nerve fibers deriving mostly from the gingival and a few periodontal nerve fibers ...	1989	267109
[diagnosis and treatment of gastrointestinal polyps].		1989	259864
the dentist as a primary medical evaluator.		1989	267332
the analgesic effect of transcutaneous electrical nerve stimulation (tns) in patients with rheumatoid arthritis. a comparative study of different pulse patterns.	transcutaneous electrical nerve stimulation (tns) was used on 20 patients with severe wrist pain due to rheumatoid arthritis. three different stimulation frequencies were used: high 70 hz stimulation (70 tns), low frequency 3 hz stimulation (3 tns) and brief trains of stimuli with an internal frequency of 70 hz and with a repetition rate of 3 hz (3-70 tns). the analgesic effect was evaluated on the patient's own estimate of pain relief and by means of a loading test in which the length of time t ...	1989	313550
[spontaneous hematoma of the duodenum. apropos of 1 case--literature review].		1989	313491
[localized enamel opacity].		1989	287242
perioperative myocardial infarction associated with coronary artery bypass graft surgery: improved sensitivity in the diagnosis within 6 hours after operation with 99mtc-glucoheptonate myocardial imaging and myocardial-specific isoenzymes.	the present study was performed to evaluate scintigraphic imaging with technetium 99m-labeled glucoheptonate and serum enzyme levels of creatine phosphokinase isoenzyme (mb-cpk) in the early diagnosis of perioperative acute myocardial infarction associated with saphenous vein bypass graft operations. myocardial imaging was done in 27 patients (50% of whom were considered high-risk) before operation and again 5 hours after operation. four of these patients (15%) had both electrocardiographic and ...	1989	313189
hypercalciuria related to cadmium exposure.	a work force has been investigated for possible cadmium intoxication. one group who are coppersmiths have an 18.5 per cent prevalence of upper urinary tract stone disease associated with a statistically highly significant hypercalciuria and reduced serum inorganic phosphate. proof of exposure to cadmium has been confirmed in all workers. the trace element cadmium should be kept in mind when investigating stone formers who exhibit an unexplained hypercalciuria.	1989	209595
sickle cell crises: psychological factors associated with onset.		1989	267253
relationship of oral complications to peripheral blood leukocyte and platelet counts in patients receiving cancer chemotherapy.	patients undergoing cancer chemotherapy often suffer from oral complications as a result of their disease and its treatment. the effects of the chemotherapy on the bone marrow and oral mucosa, coupled with the patient's immunosuppressed state and altered oral microbial flora, predispose these patients to oral mucositis, infection, and hemorrhage. the oral mucosa appears to mirror the effects of the chemotherapy on the bone marrow, as there appears to be a direct relationship between the changing ...	1989	313547
human milk immunoglobulin a antibodies to shigella virulence determinants.	because human milk is thought to protect infants from shigellosis, we evaluated milk for immunoglobulin a to shigella virulence determinants. milk was preincubated to remove antibodies unrelated to each locus of interest, using defined shigella and e. coli hybrids containing known shigella genetic segments prior to immunoblotting. the milk could not be shown to contain antibodies to chromosomally encoded virulence loci except for the expected antibodies to the products of the histidine locus. ho ...	1989	2470676
in vitro treatment of hep-2 cells with human tumor necrosis factor-alpha and human interferons reduces invasiveness of salmonella typhimurium.	enteroinvasive bacteria, like salmonella typhimurium, can be internalized in in vitro cultured epithelioidal cells, like hep-2 cells. this phenomenon is inhibited by pretreatment of cells with human tumor necrosis factor alpha (tnf-alpha) in a dose- and time-dependent manner. the effect was also reproduced in other cell types, including diploid embryo fibroblast cells. the tnf-alpha effect was neutralized by anti-tnf-alpha antibodies. no synergistic effect was produced by combinations of tnf-alp ...	1989	2472042
expression in escherichia coli k-12 of the 76,000-dalton iron-regulated outer membrane protein of shigella flexneri confers sensitivity to cloacin df13 in the absence of shigella o antigen.	one of the chromosomal segments associated with virulence in shigella flexneri encodes the production of aerobactin and the synthesis of an iron-regulated 76-kilodalton outer membrane protein believed to be the ferric-aerobactin receptor. however, s. flexneri expressing this putative aerobactin receptor, which is slightly larger than that encoded by pcolv, is insensitive to the killing action of cloacin df13, a bacteriocin which binds to other aerobactin receptor proteins and kills the cells. in ...	1989	2474501
a dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of shigella flexneri.	the expression of plasmid-encoded, invasion-related antigens lpa b, c and d of shigella flexneri was found to be positively regulated at transcriptional level by a 33kd protein produced by the previously defined, virulence-associated region 1 on the sali fragment b of the 230 kb invasion plasmid. the gene (designated virb) was identified and its nucleotide sequence determined. no ipa b or c was produced in the absence of an intact virb gene although lower levels of d were produced. the previousl ...	1989	2474742
identification of a 2-md plasmid from shigella flexneri associated with reactive arthritis.	the development of reactive arthritis, a sterile inflammatory polyarthropathy that primarily affects hla-b27 positive individuals, has been associated with previous enteric infections caused by various gram-negative bacteria. the possibility that a common bacterial epitope triggers the disease was investigated by screening a panel of documented arthritogenic shigella strains as well as 2 epidemic-associated nonarthritogenic shigella controls. a 2-md plasmid specific to the arthritogenic strains ...	1989	2475114
identification and characterization of transcription termination sites in the escherichia coli lacz gene.	the escherichia coli lacz gene contains a series of latent transcriptional terminators that are responsible for the polar effects of certain mutations. we demonstrate, using gel electrophoretic size analyses and nuclease s1 mapping procedures, that rna polymerase terminates rna synthesis in the vicinity of five positions 180, 220, 379, 421 and 463 base-pairs downstream from the start point during transcription of lacz dna in vitro in the presence of rho factor. termination at all but the 421 pos ...	1989	2475637
antihypertensive, anticalcinotic, and antiarteriosclerotic properties of anipamil, a long-acting new derivative of verapamil.	the classical indication for calcium antagonists has for a long time been coronary heart disease. however, more recent experimental data as well as a host of clinical observations now encourage a broader use of these drugs also for antihypertensive purposes and for prevention of concomitant sclerotic vascular injury. needless to say, for the treatment of hypertensive crises rapidly acting calcium antagonists are required, whereas for chronic therapy of hypertension calcium antagonists with a lon ...	1989	2475679
[development and use of solid-phase test-systems for the detection of o-antigen of shigella sonnei and shigella flexneri].	the possibility of the diagnosis of dysentery caused by s. sonnei and s. flexneri, as well as the determination of the dynamics of the distribution of specific o-antigen in the patient's body, by means of the enzyme-linked immunosorbent assay system developed on the basis of antibody preparations obtained by immunosorption has been studied. the study has shown that for better diagnosis the use of fecal extracts is preferable in assays; when used in combination with bacteriological analysis, thes ...	1989	2477970
[use of immunoenzyme analysis in the rapid diagnosis and study of the characteristics of circulating o-antigen in biological fluids of patients with acute dysentery sonne].	the data on the clinical approval of the original enzyme immunoassay system for the determination of somatic o-antigen in the blood serum and urine of patients with acute sonne dysentery are presented. the level of the antigen determined in the biological fluids of patients has been shown to depend on the severity of the disease. different types of dynamic curves, reflecting the level of o-antigen in the biological fluids of patients with acute sonne dysentery and characteristic of different cli ...	1989	2477971
[cloning of the gene relative to invasive ability of shigella flexneri 5].	a genomic library of s. flexneri 5 large plasmid (140 md) was constructed using the cosmid pjb8 as vector. there were more than 4,000 transformants in the library. 66 clones were picked out from the library by hybridization with 17kb gene probe relative to invasive ability. a few clones were analyzed. the results indicated that all these clones tested contained recombinant plasmids, they could hybridize with 17 kb gene probe. when these recombinant plasmids were digested with ecor1, 17 kb fragme ...	1989	2486252
mechanism of antigenic variation in shigella flexneri bacilli. i. selective lethal effect of normal sera on mixed population of s. flexneri 1b serotype and its antigenic 3b mutant.	normal non-immune calf, human and mice sera kill-preferentially the shigella flexneri 1b serotype cells with antigenic formula i; 3, 4, 6 leaving the antigenic mutant cells of the serotype 3b with antigenic formula iii; 3, 4, 6. the strongest lethal action was observed in normal calf serum. this serum makes possible to obtain the 3b serotypes mutant from pure population of 1b serotype cells. thermal inactivation of the complement deprives the serum of its selective activity.	1989	2487379
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
intracisternal injection of a trh analogue stimulates gastric luminal serotonin release in rats.	intracisternal injection of trh analogue, rx 77368, which has been previously shown to induce a vagal-dependent stimulation of gastric acid secretion and contractility dose-dependently (10-300 ng) stimulated serotonin release into the gastric lumen of conscious pylorus-ligated rats. the stimulation of gastric acid and serotonin release occurred 15 and 30 min, respectively, after intracisternal injection of rx 77368 (100 ng) and lasted for over 2 h, as measured in urethan-anesthetized rats with g ...	1989	2493198
renal function and morphology in sudanese patients with advanced hepatosplenic schistosomiasis and portal hypertension.	the association between glomerular disease and hepatosplenic schistosomiasis is well documented in reports from south america. during the present hospital investigation in sudan, 58 patients admitted for intercurrent complications of advanced hepatosplenic schistosomiasis were studied. the patients, median age 35 years, had no concurrent schistosoma haematobium infection. diagnostic criteria included an enlarged spleen (n = 58), at least 1 episode of hematemesis (n = 55) and/or melena (n = 36), ...	1989	2493202
in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine.	in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ...	1989	2493210
[value of determining the spectrum of blood serum fatty acids in evaluating increasing the effectiveness of antibiotic therapy of dysentery in children with prodigiozan and ephedrine].	one hundred and ninety one children with acute sonne and flexner dysentery were observed with respect to the disease process, immunity indices and blood serum fatty acid spectrum. 104 children were treated with monomycin alone and 87 children were treated with the antibiotic in combination with prodigiozan and ephedrine as immunostimulators. it was shown that the recovery terms in the patients treated with the use of the immunostimulators decreased as compared to the patients treated with the an ...	1989	2502081
hemagglutinating properties of shigella dysenteriae type 1 and other shigella species.	strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ...	1989	2503449
infections in british clinical laboratories, 1986-87.	during 1986-87 this continuing survey showed 15 specific infections in the staff of 235 laboratories, representing 28,524 person years of exposure. the community was the probable source of four of the five cases of tuberculosis and one of the five cases of salmonellosis. occupational exposure was the probable cause of four infections by shigella flexneri, three by salmonella typhimurium, and one by s typhi, all affecting medical laboratory scientific officers (mlsos) in microbiology. occupationa ...	1989	2503546
[clinical studies on patients with legal communicable diseases encountered from 1981 to 1986].	patients with legal communicable diseases admitted to kawasaki municipal hospital between 1981 and 1986 were studied. ages of patients were over 15 year-old. during this period 115 patients were admitted. out of these patients 84 had shigellosis, and 9 had amebiasis. out of 20 salmonellosis, 18 were due to salmonella typhi and 2 were due to salmonella paratyphi-a, respectively. two were admitted because of cholera. out of the patients with shigellosis, 27 were domestic and 57 were foreign infect ...	1989	2504839
[the isolation and immunologic study of ribosomal preparations from shigella flexneri].	s. flexneri ribosomal preparations were isolated by differential centrifugation or by fractionation with polyethylene glycol-6000. their chemical composition and spectrophotometric properties were characteristic of ribosomes, and, as shown by the results of the serological assay, the content of o-specific component was, on the average, 1.4%. the ribosomal preparations were nontoxic for mice when injected intraperitoneally and intravenously in large doses and induced systemic o-antibody response ...	1989	2511706
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].	in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...	1989	2518646
[in vitro cultivation of human pulpal fibroblast strains--permanent and deciduous teeth].	we succeeded in separating and the cultivating stable monolayer cultures of dental pulp fibroblast strains derived from permanent and deciduous human teeth. human permanent (n = 67) and deciduous teeth (n = 26) were extracted under acupuncture anaesthesia for the correction of malocclusion. after splitting the teeth, the pulp tissues were carefully removed, placed in tissue culture flasks, and grown in dulbecco's modified eagle medium supplemented with 10% fetal calf serum (fcs). the human pulpa ...	1989	2519279
[uropathogenic serratia marcescens of hospital origin: analysis of the adhesion mechanism of 2 strains].	we isolated 2 serratia marcescens strains, fim (0) and fim (-), from patients with urinary tract infection. adhesion properties were studied by hemagglutination, response of uroepithelial cells and electron microscopy techniques. s marcescens fim (+) showed high adherence to uroepithelial cells, hemagglutination capacity and peculiar electron microscopy characteristics. all of these are absent in the fim (-) variety. the fim (+) strain was resistant to nitrofurantoin, nalidixic acid, tetracyclin ...	1989	2519411
bidimensional reversed-phase high-pressure liquid chromatography analysis of cultured cell neuropeptides: application to atrial natriuretic factor.	we report here a one-step procedure for extraction and analysis of neuropeptides in chromaffin cell culture media and acid extracts using reversed-phase hplc. the bidimensional hplc system consists of a precolumn connected to a six-port switching valve which is on-line with an analytical column. the direct injection of the biological samples onto the precolumn previously equilibrated with 15% acetonitrile allows the elimination of interfering substances. the samples purified on the precolumn can ...	1989	2527010
determination of the secondary structure of the dna binding protein ner from phage mu using 1h homonuclear and 15n-1h heteronuclear nmr spectroscopy.	the sequential resonance assignment of the 1h and 15n nmr spectra of the dna binding protein ner from phage mu is presented. this is carried out by using a combination of 1h-1h and 1h-15n two-dimensional experiments. the availability of completely labeled 15n protein enabled us to record a variety of relayed heteronuclear multiple quantum coherence experiments, thereby enabling the correlation of proton-proton through-space and through-bond connectivities with the chemical shift of the directly ...	1989	2527559
immunogenicity of a yeast-derived hepatitis b vaccine in hemodialysis patients.	in a multicenter study of hemodialysis patients in spain, the immunogenicity of a yeast-derived recombinant deoxyribonucleic acid hepatitis b vaccine was evaluated. two different vaccination schedules were examined: zero, one, two, six months and zero, one, two, 12 months. two different dose levels (20 micrograms and 40 micrograms) were also compared. no serious adverse effects were reported by any of the vaccinees; the most frequently reported reaction was soreness at the injection site. this s ...	1989	2528295
1-(3-trifluoromethylphenyl) piperazine (tfmpp) in the ventral tegmental area reduces the effect of desipramine in the forced swimming test in rats: possible role of serotonin receptors.	1-(3-trifluoromethylphenyl)piperazine (tfmpp), a serotonin1 (5-ht1) receptor agonist, injected i.p. in doses of 0.1 and 0.6 mg/kg, did not modify the immobility time of rats in the forced swimming test but significantly antagonized the effect of a 7 days treatment with 10 mg/kg per day desipramine (dmi). a similar effect was found on infusing 1 and 5 micrograms/microliters tfmpp bilaterally into the ventral tegmental area (vta). infusion of 5 micrograms/microliters tfmpp into the nucleus accumbe ...	1989	2533079
identification of icsa, a plasmid locus of shigella flexneri that governs bacterial intra- and intercellular spread through interaction with f-actin.	the capacity of shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as intercellular movements, since this movement was inhibited by cytochalasin d and involvement of f-actin was demonstrated by direct labeling of infected cells with the specific dye n-(7-ni ...	1989	2542950
human endothelial cells inhibit platelet aggregation by separately stimulating platelet cyclic amp and cyclic gmp.	thrombin-induced platelet aggregation was monitored in a mixture of washed human platelets and cultured human endothelial cells on microcarrier beads. endothelial cells completely inhibited platelet aggregation and enhanced the content of both cyclic amp and cyclic gmp in the platelets. inhibition of endothelial prostacyclin synthesis with indomethacin abolished the cyclic amp increase in the platelets, whereas the cyclic gmp increase was unimpaired. a significant component of the endothelial an ...	1989	2546782
characterization of cdna clones encoding two putative isoforms of the alpha 1 subunit of the dihydropyridine-sensitive voltage-dependent calcium channel isolated from rat brain and rat aorta.	cdna clones encoding rat brain and rat aorta isoforms of the alpha 1 subunit of the dihydropyridine-sensitive, voltage-dependent calcium channel were isolated and sequenced. these tissue-specific cdna clones share significant amino acid similarity with the rabbit skeletal muscle calcium channel alpha 1 subunit (75% and 66% amino acid identity for rat brain and rat aorta isoforms, respectively). northern analysis revealed transcript sizes of 6.5 and 8.6 kb in aorta and 8.6 kb in brain.	1989	2546805
influence of non-esterified fatty acids on respiratory control of reconstituted cytochrome-c oxidase.	bovine heart cytochrome-c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non-esterified fatty acids. palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 microm, when measured using a spectrophotometric procedure but not with a polarographic assay method. at higher fatty acid concentrations no further change was found. a 50% dec ...	1989	2546826
isolation and characterisation of calcineurin from adrenal cell cytoskeleton: identification of substrates for ca2+-calmodulin-dependent phosphatase activity.	ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of y-1 mouse adrenal and bovine fasciculata cells. the activity is inhibited by three inhibitors of calmodulin (trifluoperazine, w-7 and pimozide) with ec50 in the low micromolar range. protein phosphatase activity is inhibited by vanadate, fluoride, zn2+ and pyrophosphate, stimulated by mn2+ and found to be tightly bound to the cytoskeleton. substrates for endogenous phosphatase activity were defined by one- and tw ...	1989	2546840
hypoglycaemia induced by antibodies to insulin receptor following a bone marrow transplantation in an immunodeficient child.	severe hypoglycaemia developed seven months after a bone marrow transplantation in a child with severe combined immunodeficiency. his serum exerted potent insulin-like activity: (a) it stimulated insulin receptor autophosphorylation and kinase activity in cell-free systems, this effect being additive to insulin; (b) it increased glucose transport in isolated soleus muscle. these insulin-like effects were due to immunoglobulins against the insulin receptor. indeed, the patient serum immunoprecipi ...	1989	2546845
human papillomavirus and cervical cancer. summary report.		1989	2546883
functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in shigella flexneri 2a.	the 7 kb virulence region-2 of the large (virulence) plasmid in shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. insertion and deletion mutagenesis, dna subcloning and sds-polyacrylamide gel electrophoresis of proteins synthesized in minicells demonstrated five genes in this region. they encode 24, 18, 62 (ipab), 41 (ipac) and 37 (ipad)-kilodalton (kd) proteins. complementation of tn5-induced mutations in region-2 with the above plasmid construc ...	1989	2552264
thyrotropic activity of the ovine corticotropin-releasing factor in the chick embryo.	an injection of several doses of ovine corticotropin-releasing factor (ocrf) was made into an allantoic blood vessel of 18-day-old chick embryos. all doses used (0.5, 1, 2, and 5 micrograms) induce a quick increase of plasma corticosterone concentrations after 15 and 30 min and 1 and 2 hr and this in a dose-dependent manner with the high doses having a prolonged effect lasting up to 2 hr. an increase in plasma levels of thyroxine (t4) and triiodothyronine (t3) was observed after 1 and 2 hr. afte ...	1989	2555247
autotransplantation of avian parathyroid glands: an animal model for studying parathyroid function.	the parathyroid glands of chickens were autotransplanted and the return of parathyroid function following transplantation was determined. parathyroidectomy (ptx) resulted in a marked hypocalcemia (5.2 +/- 0.2 mg/dl) 4 hr following ptx. plasma calcium (pca) had declined to 4.3 +/- 0.2 mg/dl 24 hr after ptx. parathyroid glands were transplanted subcutaneously 24 hr after removal and 24 hr later, pca had risen to 8.6 +/- 0.5 mg/dl. seven days after ptx. pca increased to 10.3 +/- 0.2 mg/dl and by 14 ...	1989	2555250