Publications

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role for fks1 in the intrinsic echinocandin resistance of fusarium solani as evidenced by hybrid expression in saccharomyces cerevisiae.the opportunistic mold fusarium solani is intrinsically resistant to cell wall synthesis-inhibiting echinocandins (ecs), including caspofungin and micafungin. mutations that confer acquired ec resistance in saccharomyces cerevisiae and other normally susceptible yeast species have been mapped to the fks1 gene; among these is the mutation of residue 639 from phe to tyr (f639y) within a region designated hot spot 1. fks1 sequence analysis identified the equivalent of y639 in f. solani as well as i ...200919258277
microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bdha) used against agricultural plant pathogenic fungi.microbial modification of polyunsaturated fatty acids can often lead to special changes in their structure and in biological potential. therefore, the aim of this study was to develop potential antifungal agents through the microbial conversion of docosahexaenoic acid (dha). bioconverted oil extract of docosahexaenoic acid (bdha), obtained from the microbial conversion of docosahexaenoic acid (dha) by pseudomonas aeruginosa pr3, was assessed for its in vitro and in vivo antifungal potential. myc ...200919259715
multipurpose solutions and rates of biocidal efficacy.to investigate the hypothesis that reasonable levels of disinfection are achievable over relatively short exposure durations of microorganisms to multipurpose solutions, this study examined the stand-alone disinfection efficacy at 10, 20, and 30 min of five different multipurpose solutions products, each against five different pathogens eye care practitioners may encounter in their contact lens practice.200919265330
[fusarium keratitis and endophthalmitis treated by intravenous ambisome].this is a case study of fusarium keratitis progressing to endophthatmitis that was successfully treated with a tiposomal formulation of amphotericin b (ambisome] and local natamycin 5%.200919320385
tracking the emerging human pathogen pseudallescheria boydii by using highly specific monoclonal antibodies.pseudallescheria boydii has long been known to cause white grain mycetoma in immunocompetent humans, but it has recently emerged as an opportunistic pathogen of humans, causing potentially fatal invasive infections in immunocompromised individuals and evacuees of natural disasters, such as tsunamis and hurricanes. the diagnosis of p. boydii is problematic since it exhibits morphological characteristics similar to those of other hyaline fungi that cause infectious diseases, such as aspergillus fu ...200919321690
biological screening of euphorbia helioscopia l.the present research is preliminary biological screening of euphorbia helioscopia l. (euphorbiaceae). dichloromethane and methanol extracts of the aerial parts of the plant were investigated for their antioxidant, antifungal, antibacterial and phytotoxic activities. dichloromethane extract exhibited significant activity against fusarium solani with 90% inhibition, where as the same extract also showed non-significant activity against salmonella typhi and bacillus subtilis. methanolic extract has ...200919339230
antimicrobial dihydroisocoumarins from crassocephalum biafrae.bioassay-guided fractionation of the chcl (3)-soluble extract of the stem bark of crassocephalum biafrae (asteraceae) resulted in the isolation of three new dihydroisocoumarins, named biafraecoumarins a ( 1), b ( 2), and c ( 3); two known triterpenes ( 4 and 5); and a known ceramide ( 6). the structures of the new compounds were established as 7-but-15-enyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 1), 7-butyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 2), and 7-butyl- ...200919350487
soft contact lens disinfection solution efficacy: clinical fusarium isolates vs. atcc 36031.to compare the disinfecting efficacy of five soft contact lens multipurpose disinfection solutions (mpds) against fusarium solani clinical isolates and the iso standard atcc 36031 strain.200919384259
impact of biocide treatments on the bacterial communities of the lascaux cave.the lascaux cave contains a remarkable set of paintings from the upper palaeolithic. shortly after discovery in 1940, the cave was modified for public viewing and, in 2001, was invaded by a fusarium solani species complex. benzalkonium chloride was used from 2001 to 2004 to eliminate the fungal outbreak. in this study, we carried out a sampling in most of the cave halls and galleries. sequence analysis and isolation methods detected that the most abundant genera of bacteria were ralstonia and ps ...200919404600
chromatographic isolation and characterization of a novel peroxidase from large lima legumes.a novel peroxidase with antifungal activity was isolated from the large lima bean (phaseolus limensis) seeds. the procedure entailed extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-toyopearl, and gel filtration on sephadex g-75. the enzyme was adsorbed on affi-gel blue gel and sp-toyopearl, and possessed a molecular weight of 34 kda in sds-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions. ...200919408412
relative in vitro rates of attachment and penetration of hydrogel soft contact lenses by haplotypes of fusarium.to investigate the relative abilities of different haplotypes of the fusarium solani (fssc)-fusarium oxysporum (fosc) complexes to attach to and invade hydrogel contact lenses.200919411965
synthesis of alkyl esters by cutinase in miniemulsion and organic solvent media.the main objective of this work was studying and testing the nature and influence of reaction media (organic solvent vs. miniemulsion system) on the synthesis of alkyl esters catalyzed by fusarium solani pisi cutinase. ester synthesis and cutinase selectivity for different chain length of acids and alcohols (ethyl and hexyl) were evaluated. in iso-octane, after 1 h of reaction, cutinase exhibits rates of esterification between 0.24 micromol x mg(-)1 x min(-1) for ethyl oleate and 1.15 micromol x ...200919418474
fungal nitrilases as biocatalysts: recent developments.of the numerous putative fungal nitrilases available from protein databases only a few enzymes were purified and characterized. the purified nitrilases from fusarium solani, fusarium oxysporum f. sp. melonis and aspergillus niger share a preference for (hetero)aromatic nitriles, temperature optima between 40 and 50 degrees c and ph optima in the slightly alkaline region. on the other hand, they differ in their chemoselectivity, i.e. their tendency to produce amides as by-products. the production ...200919427375
cutaneous fusarium solani infection in childhood acute lymphoblastic leukaemia.cutaneous involvement is often an initial presentation of infection with fusarium species, which occurs more commonly in immunocompromised hosts and may be either localized or widespread. skin lesions typically appear as red or grey macules, which may develop central ulceration and black eschar. secondary dissemination to extracutaneous organs may occur in immunocompromised hosts, especially those with prolonged and severe neutropenia. we describe a case of widespread cutaneous involvement after ...200919438533
use of mass spectrometry to identify clinical fusarium isolates.fusarium spp. have recently emerged as significant human pathogens. identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. in this study, 62 strains, or isolates, belonging to nine fusarium species were subjected to both molecular identification tef1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (maldi-to ...200919456834
fusarium keratitis in mexico.to describe the clinical characteristics, diagnosis, treatment, and visual outcome of 61 cases of fusarium keratitis in mexico.200919512910
crystal structure and enhanced activity of a cutinase-like enzyme from cryptococcus sp. strain s-2.the structural and enzymatic characteristics of a cutinase-like enzyme (cle) from cryptococcus sp. strain s-2, which exhibits remote homology to a lipolytic enzyme and a cutinase from the fungus fusarium solani (fs cutinase), were compared to investigate the unique substrate specificity of cle. the crystal structure of cle was solved to a 1.05 a resolution. moreover, hydrolysis assays demonstrated the broad specificity of cle for short and long-chain substrates, as well as the preferred specific ...200919544571
continuous hydrolysis of 4-cyanopyridine by nitrilases from fusarium solani o1 and aspergillus niger k10.the operational stabilities of nitrilases from aspergillus niger k10 and fusarium solani o1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (csmrs). the former enzyme was fairly stable at 30 degrees c with a deactivation constant (k (d)) and enzyme half-life of 0.014 h(-1) and 50 h, respectively, but the latter exhibited an even higher stability characterized by k (d) = 0.008 h(-1) and half-life of 87 h at 40 degrees c. another advantage of this enzyme ...200919554325
molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested polymerase chain reaction based on 28s ribosomal rna gene sequences.onychomycosis is often caused by dermatophytes, but the role of nondermatophytes is underestimated due to the difficulty of identifying them by conventional direct microscopy and culture.200919566663
[preliminary study on the use of dna suspension array for the identification of corneal pathogenic fungi].to develop a multiplex, microsphere-based dna suspension array for the identification of important pathogenic fungi of cornea and to study the feasibility of its application in the clinical diagnosis of fungal keratitis.200919573328
antifungal activity of nettle (urtica dioica l.), colocynth (citrullus colocynthis l. schrad), oleander (nerium oleander l.) and konar (ziziphus spina-christi l.) extracts on plants pathogenic fungi.anti-mycotic activity of the ethanol extracts from nettle (urtica dioica l.), colocynth (citrullus colocynthis l. schrad), konar (ziziphus spina-christi l.) and oleander (nerium oleander l.) floral parts were screened in vitro against four important plant pathogenic fungi viz.; alternaria alternate, fusarium oxysporum, fusarium solani and rizoctonia solani using agar dilution bioassay. extracts showed antifungal activity against all the tested fungi. among the plants, nettle and colocynth were t ...200919579919
induce systemic resistance in lupine against root rot diseases.root rot caused by soil borne pathogenic fungi is the most sever disease attacks lupine plants. isolation trials from diseased plants in some areas of dakahlia province (egypt) was carried out. rhizoctonia solani and fusarium solani proved to be the most dominant isolates. meanwhile, fusarium oxysporum and sclerotium rolfsii were less frequent. efficacies of some plant resistance elicitors viz.: chitosan (chi), salicylic acid (sa) and hydroquinone (hq) in comparing to the fungicide rhizolex t-50 ...200919579949
flash photolysis of cutinase: identification and decay kinetics of transient intermediates formed upon uv excitation of aromatic residues.aromatic amino acids play an important role in ultraviolet (uv)-induced photochemical reactions in proteins. in this work, we aim at gaining insight into the photochemical reactions induced by near-uv light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, fusarium solani pisi cutinase, a lipolytic enzyme. with this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (trp) and lysozyme as refe ...200919580759
high-level expression and characterization of fusarium solani cutinase in pichia pastoris.high-level extracellular production of fusarium solani cutinase was achieved using a pichia pastoris expression system. the cutinase-encoding gene was cloned into ppiczalphaa with the saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter by two different ways. the additional sequences of the c-myc epitope and (his)6-tag of the vector were fused to the c-terminus of cutinase, while the other expression vector was constructed without any additional s ...200919580870
enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules.a lipase from thermomyces lanuginosus and cutinases from thermobifida fusca and fusarium solani hydrolysed poly(ethylene terephthalate) (pet) fabrics and films and bis(benzoyloxyethyl) terephthalate (3pet) endo-wise as shown by maldi-tof-ms, lc-uvd/ms, cationic dyeing and xps analysis. due to interfacial activation of the lipase in the presence of triton x-100, a seven-fold increase of hydrolysis products released from 3pet was measured. in the presence of the plasticizer n,n-diethyl-2-phenylace ...200919616594
genotypic identification of fusarium species from ocular sources: comparison to morphologic classification and antifungal sensitivity testing (an aos thesis).ocular infections caused by fungal organisms can cause significant ocular morbidity, particularly when diagnosis and treatment are delayed. rapid and accurate identification of fusarium species at the subgenus level using current diagnostic standards is timely and insensitive. the purpose of this study is to examine the usefulness of polymerase chain reaction (pcr) analysis of the internal transcribed spacer (its) regions (its1, 5.8s, and its2) in detecting and differentiating fusarium species f ...200819277239
identification and characterization of bacterial cutinase.cutinase, which exists in both fungi and bacteria, catalyzes the cleavage of the ester bonds of cutin. fungal cutinases have been extensively studied, however, reports on bacterial cutinases have been limited due to the lack of knowledge concerning the identity of their open reading frames. in the present study, the cutinase from thermobifida fusca was induced by cutin and purified to homogeneity by following p-nitrophenyl butyrate hydrolyzing activity. peptide mass fingerprinting analysis of th ...200818658138
microbial community analysis of field-grown soybeans with different nodulation phenotypes.microorganisms associated with the stems and roots of nonnodulated (nod(-)), wild-type nodulated (nod(+)), and hypernodulated (nod(++)) soybeans [glycine max (l.) merril] were analyzed by ribosomal intergenic transcribed spacer analysis (risa) and automated risa (arisa). risa of stem samples detected no bands specific to the nodulation phenotype, whereas risa of root samples revealed differential bands for the nodulation phenotypes. pseudomonas fluorescens was exclusively associated with nod(+) ...200818658280
in-vitro antibacterial, antifungal activity of some transition metal complexes of thiosemicarbazone schiff base (hl) derived from n4-(7'-chloroquinolin-4'-ylamino) thiosemicarbazide.the synthetic, spectroscopic, and biological studies of cu(ii), ni(ii), zn(ii), co(ii), mn(ii), fe(iii) and cr(iii) complexes of n(4)-(7'-chloroquinolin-4'-ylamino)-n(1)-(2-hydroxy-benzylidene)thiosemicarbazone (hl) obtained by the reaction of n(4)-(7'-chloroquinolin-4'-ylamino)thiosemicarbazide with 2-hydroxybenzaldehyde. the structures of the complexes were determined on the basis of the elemental analyses, spectroscopic data (ir, electronic, (1)h and (13)c nmr and mass spectra) along with mag ...200818665996
first isolation of a novel thermostable antifungal peptide secreted by aspergillus clavatus.a novel antifungal peptide produced by an indigenous fungal strain (vr) of aspergillus clavatus was purified. the antifungal peptide was enriched in the supernatant after heat treatment at 70 degrees c. the thermostable character was exploited in the first purification step, as purified peptide was obtained after ultrafiltration and reverse phase-hplc on c18 column application. the purified peptide named "acafp" for a. clavatus antifungal peptide, has molecular mass of 5773da determined by maldi ...200818687373
activities of antifungal agents against yeasts and filamentous fungi: assessment according to the methodology of the european committee on antimicrobial susceptibility testing.we compared the activities of antifungal agents against a wide range of yeasts and filamentous fungi. the methodology of the european committee on antimicrobial susceptibility testing (eucast) for yeasts and spore-forming molds was applied; and a total of 349 clinical isolates of candida spp., other yeast species, aspergillus spp., and nondermatophyte non-aspergillus spp. were investigated. the average geometric mean (gm) of the mics of the various drugs for candida spp. were as follows: amphote ...200818694949
following multi-component reactions in liquid medium using spectral band-fitting techniques.the kinetics of the hydrolysis reaction of p-nitrophenyl butyrate catalyzed by a single-site mutant of fusarium solani pisi cutinase, supported on zeolite nay by adsorption, was followed using ultraviolet-visible (uv/vis) in situ diode array spectrophotometry. spectral band-fitting techniques allowed the use of in situ diode array spectrophotometry to obtain quantitative data on the concentrations of the various species present in an aqueous reaction medium even in the presence of the solid cata ...200818702868
ctf1, a transcriptional activator of cutinase and lipase genes in fusarium oxysporum is dispensable for virulence.cutinolytic enzymes are secreted by fungal pathogens attacking the aerial parts of the plant, to facilitate penetration of the outermost cuticular barrier of the host. the role of cutinases in soil-borne root pathogens has not been studied thus far. here we report the characterization of the zinc finger transcription factor ctf1 from the vascular wilt fungus fusarium oxysporum, a functional orthologue of ctf1alpha that controls expression of cutinase genes and virulence in the pea stem pathogen ...200818705871
determination of in vitro susceptibility of ocular fusarium spp. isolates from keratitis cases and comparison of clinical and laboratory standards institute m38-a2 and e test methods.we evaluated the susceptibility of 85 fusarium spp. isolates from cases of fungal keratitis with 8 antifungal drugs using the standard clinical and laboratory standards institute broth microdilution and e test methods. members of the fusarium solani species complex showed consistently higher mics to the triazole drugs itraconazole, voriconazole, and posaconazole than did members of other species complexes (fusarium oxysporum and other minor species). high mics to amphotericin b, natamycin, and e ...200818707841
effects of water potential on spore germination and viability of fusarium species.germination of macroconidia and/or microconidia of 24 strains of fusarium solani, f. chlamydosporum, f. culmorum, f. equiseti, f. verticillioides, f. sambucinum, f. oxysporum and f. proliferatum isolated from fluvial channels and sea beds of the south-eastern coast of spain, and three control strains (f. oxysporum isolated from affected cultures) was studied in distilled water in response to a range of water potentials adjusted with nacl. (0, -13.79, -41.79, -70.37, -99.56 and -144.54 bars). the ...200818712540
the interactive effects of temperature and osmotic potential on the growth of marine isolates of fusarium solani.the mycelial growth of 18 fusarium solani strains isolated from sea beds of the south-eastern coast of spain was tested on potato-dextrose-agar adjusted to different osmotic potentials with either kcl or nacl (-1.50 to -144.54 bars) in 10 degrees c intervals ranging from 15 to 35 degrees c. fungal growth was determined by measuring colony diameter after 4 days of incubation. mycelial growth was maximal at 25 degrees c. the quantity and frequency pattern of mycelial growth of f. solani differ sig ...200818712541
loop grafting of bacillus subtilis lipase a: inversion of enantioselectivity.lipases are successfully applied in enantioselective biocatalysis. most lipases contain a lid domain controlling access to the active site, but bacillus subtilis lipase a (lipa) is a notable exception: its active site is solvent exposed. to improve the enantioselectivity of lipa in the kinetic resolution of 1,2-o-isopropylidene-sn-glycerol (ipg) esters, we replaced a loop near the active-site entrance by longer loops originating from fusarium solani cutinase and penicillium purpurogenum acetylxy ...200818721749
lignin degradation in wood-feeding insects.the aromatic polymer lignin protects plants from most forms of microbial attack. despite the fact that a significant fraction of all lignocellulose degraded passes through arthropod guts, the fate of lignin in these systems is not known. using tetramethylammonium hydroxide thermochemolysis, we show lignin degradation by two insect species, the asian longhorned beetle (anoplophora glabripennis) and the pacific dampwood termite (zootermopsis angusticollis). in both the beetle and termite, signific ...200818725643
phenomenological model of fungal biofilters for the abatement of hydrophobic vocs.this work describes the growth of filamentous fungi in biofilters for the degradation of hydrophobic vocs. the study system was n-hexane and fusarium solani b1. the system is mathematically described and the main physical, kinetic data and morphological parameters were obtained by independent experiments and validated with data from laboratory experiments. the model describes the increase in the transport area by the growth of the filamentous cylindrical mycelia and its relation with n-hexane el ...200818781682
a thermostable chitinase with chitin-binding activity from phaseolus limensis.a 28.6-kda chitinase with chitin-binding activity was isolated from the large lima bean (phaseolus limensis) seeds. the procedure entailed extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, and high-performance liquid chromatography (hplc) on sp-toyopearl. there was an almost 108-fold increase in specific activity of the purified chitinase compared with that of the crude extract. the enzyme exhibited a pi of 7.8 by isoelectric focusing electrophoresis. the ...200818782228
isolation, characterization and cloning of a cdna encoding a new antifungal defensin from phaseolus vulgaris l. seeds.the pvd1 defensin was purified from phaseolus vulgaris (cv. pérola) seeds, basically as described by terras et al. [terras frg, schoofs hme, de bolle mfc, van leuven f, ress sb, vanderleyden j, cammue bpa, broekaer twf. analysis of two novel classes of plant antifungal proteins from radish (raphanus sativus l.) seeds. j biol chem 1992;267(22):15301-9], with some modifications. a deae-sepharose, equilibrated with 20mm tris-hcl, ph 8.0, was initially utilized for the separation of peptides after a ...200818786582
fungal endophthalmitis: fourteen years' experience from a centre in india.background:: as fungal endophthalmitis is an emerging challenge, the study was carried out to determine the prevalence and the spectrum of fungal agents causing endophthalmitis from a single center, to identify the risk factors, and to correlate clinical course of illness with the agents involved. methods:: the microbiological and clinical records of all fungal endophthalmitis diagnosed during january 1992 through december 2005 at a tertiary center in india were reviewed retrospectively. during ...200818791488
fusarium species detected in onychomycosis in colombia.fusarium spp. have frequently been isolated from patients with onychomycosis. in colombia, several studies have shown that fusarium is the most common non-dermatophyte mould causing onychomycosis and its spread has increased in the past years. in this study, samples were collected in 2003 and 2004 from 137 patients who were diagnosed with onychomycosis caused by fusarium spp. three species of fusarium were identified: fusarium solani (64.9%), fusarium oxysporum (32.8%) and fusarium verticillioid ...200818793261
[disseminated fusariosis in a patient with acute lymphoblastic leukaemia].invasive mould infections are a major cause of infectious mortality in highly immunosuppressed patients. incidence in this high risk group is 10-20% with a death rate in excess of 50%. most invasive moulds are aspergillus spp. we present a case of a 74-year-old woman with acute lymphoblastic leukaemia who developed a rare disseminated mould infection with fusarium solani during induction chemotherapy. we present the case story and discuss the pathogenesis, clinical characteristics and treatment ...200818796288
dual elicitation for improved production of withaferin a by cell suspension cultures of withania somnifera.high yielding transformed callus culture of w. somnifera was established by infecting hypocotyls with agrobacterium tumefaciens mtcc-2250. maximum withaferin a content of 0.0875 mg/g dry cell weight and transformation efficiency of 80% were obtained. confirmation of transformation was done on the basis of the presence of the ags gene by using polymerase chain reaction. various abiotic elicitors (arachidonic acid, methyl jasmonate, calcium chloride, and copper sulfate) and biotic elicitors (cell ...200818449479
screening of tropical fungi producing polyethylene terephthalate-hydrolyzing enzyme for fabric modification.microfungi were selectively isolated for production of polyethylene terephthalate (pet) fiber-degrading enzymes potentially to be used to modify the surface of polyester fabric. a range of fungi were isolated from plant surfaces and soil samples using a polycaprolactone (pcl) plate-clearing assay technique, and screened for cutinolytic esterase (cutinase) activity. twenty-two of 115 isolates showed clearing indicating the production of cutinase. the ability of the fungi to produce cutinase in mi ...200818449587
quantification of fusarium solani f. sp. glycines isolates in soybean roots by colony-forming unit assays and real-time quantitative pcr.fusarium solani f. sp. glycines (fsg; syn. f. virguliforme akoi, o'donnell, homma & lattanzi) is a soil-borne fungus that infects soybean roots and causes sudden death syndrome (sds), a widespread and destructive soybean disease. the goal of this study was to develop and use a real-time quantitative polymerase chain reaction (qpcr) assay to compare the accumulation of genomic dna among 30 fsg isolates in inoculated soybean roots. isolates differed significantly (p < or = 0.05) in their dna accum ...200818461301
development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis.aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only to candida species as a cause of invasive fungal infections in immunocompromised humans. current immunodiagnostic tests for invasive aspergillosis (ia) are based on the detection of circulating galactomannan (gm) in a patient's serum by using a rat monoclonal antibody (mab), eb-a2, that binds to tetra (1-->5)-beta-d-galactofuranoside, the immunodominant epitope in gm. the potential cross-reactivity of mab eb-a2 with ...200818463222
probing the microenvironment of sol-gel entrapped cutinase: the role of added zeolite nay.cutinase, an esterase from fusarium solani pisi, was immobilized in sol-gel matrices of composition 1:5 tetramethoxysilane (tmos):n-alkyltrimethoxysilane (n-alkyltms). fluorescence spectroscopy using the single tryptophan (trp-69) residue of cutinase as a probe revealed that the polarity of the matrices decreased as their hydrophobicity increased up to the tmos/n-butyltms pair, which correlates with an increase in cutinase activity. fluorescence emission was suppressed (a higher than two orders ...200818490069
combined therapy in treatment of murine infection by fusarium solani.we evaluated the efficacy of voriconazole, amphotericin b and micafungin alone and combined in a murine model of disseminated infection by fusarium solani.200818495651
late-onset infections after lasik.to report two cases of infectious keratitis that developed 2 and 6 years after lasik.200818500093
caspofungin-mediated beta-glucan unmasking and enhancement of human polymorphonuclear neutrophil activity against aspergillus and non-aspergillus hyphae.we investigated whether caspofungin and other echinocandins have immune-enhancing properties that influence human polymorphonuclear neutrophil (pmn)-mediated mold hyphal damage.200818500936
biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant aot.the reactivity, stability and unfolding of wild-type (wt) fusarium solani pisi cutinase and l153q, s54d and t179c variants were studied in the absence and presence of the dioctyl sulfosuccinate sodium salt (aot) surfactant. in the absence of surfactant the s54d variant catalytic activity is similar to that of the wt cutinase, whereas l153q and t179c variants show a lower activity. aot addition induces an activity reduction for wt cutinase and its variants, although for low aot concentrations a s ...200818501723
a pseudo-outbreak of fusarium solani in an intensive care unit associated with bronchoscopy. 200818511154
molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the fusarium solani species complex.members of the species-rich fusarium solani species complex (fssc) are responsible for approximately two-thirds all fusarioses of humans and other animals. in addition, many economically important phytopathogenic species are nested within this complex. due to their increasing clinical relevance and because most of the human pathogenic and plant pathogenic fssc lack latin binomials, we have extended the multilocus haplotype nomenclatural system introduced in a previous study (d. c. chang, g. b. g ...200818524963
production of paclitaxel by fusarium solani isolated from taxus celebica.a fungus was isolated from the stem cuttings of taxus celebica, which produced paclitaxel in liquid-grown cultures. the fungus was identified as fusarium solani based on colony characteristics, morphology of conidia and the 26s rdna sequence. paclitaxel was identified by chromatographic and spectroscopic comparison with authentic paclitaxel and its cytotoxic activity towards jurkat cells in vitro.200818535360
chemical composition and antifungal activity of essential oil and various extract of silene armeria l.the aims of this study were to examine the chemical composition of the essential oil isolated from the floral parts of silene armeria l. by hydrodistillation, and to test the efficacy of essential oil and various leaf extracts (n-hexane, chloroform, ethyl acetate and methanol) as an antifungal potential. the gc-ms analysis determined that 28 compounds, which represented 89.03% of total oil, were present in the oil containing mainly 1-butene, methylcyclopropane, 2-butene and caryophyllene oxide. ...200818538562
in vitro interactions of antifungal agents against clinical isolates of fusarium spp.the in vitro activities of amphotericin b (amb), itraconazole (itc), voriconazole (vcz) and terbinafine (tbf) alone and in the combinations amb+vcz, tbf+itc and tbf+vcz were evaluated against 29 clinical isolates of fusarium spp. (15 fusarium solani, 7 fusarium oxysporum, 2 fusarium decemcellulare, 2 fusarium dimerum and 3 other fusarium spp.). minimum inhibitory concentrations were determined using the method of the clinical and laboratory standards institute and the interaction activity was ca ...200818054469
properties and applications of microbial d-amino acid oxidases: current state and perspectives.d: -amino acid oxidase (daao) is a biotechnologically relevant enzyme that is used in a variety of applications. daao is a flavine adenine dinucleotide-containing flavoenzyme that catalyzes the oxidative deamination of d-isomer of uncharged aliphatic, aromatic, and polar amino acids yielding the corresponding imino acid (which hydrolyzes spontaneously to the alpha-keto acid and ammonia) and hydrogen peroxide. this enzymatic activity is produced by few bacteria and by most eukaryotic organisms. i ...200818084756
immunological diversity within a family of cutinase-like proteins of mycobacterium tuberculosis.secreted proteins of mycobacterium tuberculosis play key roles in the assembly of the mycobacterial cell wall, with many being major targets of the host immune response. to date, meaningful characterization of a significant proportion of this important group of proteins is lacking. among the group of putatively secreted proteins of m. tuberculosis are 7 cutinase-like proteins (clp), not previously characterized in terms of their immunogenicity or vaccine protective efficacy. although the clp var ...200818565629
myd88 regulation of fusarium keratitis is dependent on tlr4 and il-1r1 but not tlr2.the fungal pathogens fusarium solani and fusarium oxysporum cause severe corneal disease in the united states and worldwide and were the causative organisms in a recent outbreak of contact lens-associated keratitis. to characterize innate immunity in fusarium keratitis, we developed a murine model in which conidia are injected into the corneal stroma. immunocompetent c57bl/6 mice rapidly developed severe corneal opacification associated with neutrophil infiltration and clearance of fusarium hyph ...200818566426
in silico comparison of transcript abundances during arabidopsis thaliana and glycine max resistance to fusarium virguliforme.sudden death syndrome (sds) of soybean (glycine max l. merr.) is an economically important disease, caused by the semi-biotrophic fungus fusarium solani f. sp. glycines, recently renamed fusarium virguliforme (fv). due to the complexity and length of the soybean-fusarium interaction, the molecular mechanisms underlying plant resistance and susceptibility to the pathogen are not fully understood. f. virguliforme has a very wide host range for the ability to cause root rot and a very narrow host r ...200818831797
mold species in dust from the international space station identified and quantified by mold-specific quantitative pcr.dust was collected over a period of several weeks in 2007 from hepa filters in the u.s. laboratory module of the international space station (iss). the dust was returned on the space shuttle atlantis, mixed, sieved and the dna was extracted. using a dna-based method called mold-specific quantitative pcr (msqpcr), 39 molds were measured in the dust. potential opportunistic pathogens aspergillus flavus and aspergillus niger and potential moderate toxin producers penicillium chrysogenum and penicil ...200818602989
use of multiple methods for genotyping fusarium during an outbreak of contact lens associated fungal keratitis in singapore.in singapore, an outbreak of fungal keratitis caused by members of the fusarium solani species complex (fssc) was identified in march 2005 to may 2006 involving 66 patients. epidemiological investigations have indicated that improper contact lens wear and the use of specific contact lens solutions were risk factors.200818627616
fungistatic activity of iron-free bovin lactoferrin against several fungal plant pathogens and antagonists.lactoferrin (lf) is a member of the transferrin family of iron-binding glycoproteins. it is also a multifunctional protein of 80 kda that is synthesized by glandular epithelial cells and secreted into mucosal fluid. high levels of lf are present in colostrom and milk and low levels in tears, saliva, and gastrointestinal and reproductive secretions. data regarding the antifungal effects of lf are limited. studies have been performed on candida albicans, which demonstrated that lf inhibits the gro ...200818629710
multiplex tandem pcr: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.we describe the first development and evaluation of a rapid multiplex tandem pcr (mt-pcr) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. the assay uses a short-cycle multiplex amplification, followed by 12 simultaneous pcrs which target the fungal internal transcribed spacer 1 (its1) and its2 region, elongation factor 1-alpha (ef1-alpha), and beta-tubulin genes to identify 11 fungal pathogens: candida albicans, candid ...200818632914
fungal endophthalmitis: fourteen years' experience from a center in india.as fungal endophthalmitis is an emerging challenge, the study was carried out to determine the prevalence and the spectrum of fungal agents causing endophthalmitis from a single center, to identify the risk factors, and to correlate clinical course of illness with the agents involved.200819009680
genetic variability and relationships among seventeen trichoderma isolates to control dry root rot disease using rapd markers.trichoderma spp. has been identified as potential antagonist of fusarium solani, which is causing dry root rot of citrus. a random amplified polymorphic dna (rapd) marker was used to estimate the genetic variation among 17 isolates of trichoderma. these isolates were characterized using 20 random primers of the opm series, out of which 16 primers gave a total of 145 dna fragments, showing 91.8% polymorphism. the genetic distance between each isolate was calculated, and cluster analysis was used ...200819040115
pea-fusarium solani interactions contributions of a system toward understanding disease resistance.this mini-review points to the usefulness of the pea-fusarium solani interaction in researching the biochemical and molecular aspects of the nonhost resistance components of peas. this interaction has been researched to evaluate the resistance roles of the phytoalexin, pisatin, the cuticle barrier, and the activation of the nonhost resistance response. concurrently, evaluations of associated signaling processes and the tools possessed by the pathogen to contend with host obstacles were included. ...200818944184
effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by fusarium solani in a rabbit model: a pilot study.to evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental fusarium solani keratitis in rabbits.200819169521
[effect of organic amendments on soil fungi community and continuous cropping panax quinquefolium].to study the effect of different organic amendments treated replant soil and to find the relations between the soil fungi community and the growth of panax quinquefolium l. seedling.200818973004
[cloning of a homologous gene of magnaporthe grisea pmk1 type mapk from ustilaginoidea virens and functional identification by complement in magnaporthe grisea corresponding mutant].cloning of a homologous gene of pmk1 type mitogen-activated protein kinase (mapk) from the rice false smut fungus ustilaginoidea virens.200819149162
chemical composition, antioxidant and antimicrobial activities of basil (ocimum basilicum) essential oils depends on seasonal variations.chemical composition, antioxidant and antimicrobial activities of the essential oils from aerial parts of basil (ocimum basilicum l.) as affected by four seasonal, namely summer, autumn, winter and spring growing variation were investigated. the hydro-distilled essential oils content ranged from 0.5% to 0.8%, the maximum amounts were observed in winter while minimum in summer. the essential oils consisted of linalool as the most abundant component (56.7-60.6%), followed by epi-α-cadinol (8.6-11. ...200826065762
post-traumatic fungal endophthalmitis--a prospective study.to study the incidence, clinical presentation, and the response of anti-fungals in cases of fungal endophthalmitis following open globe injury.200816751752
discovery of fusarium solani as a naturally occurring pathogen of sugarbeet root maggot (diptera: ulidiidae) pupae: prevalence and baseline susceptibility.the fungus fusarium solani (mart.) sacc. was discovered as a native entomopathogen of the sugarbeet root maggot, tetanops myopaeformis (röder), in the red river valley of north dakota during the 2004 sugarbeet production season. this is the first report of a native pathogen affecting the pupal stage of t. myopaeformis. forty-four percent of larvae collected from a field site near st. thomas (pembina co.) in northeastern north dakota during may and june of 2004 were infected with the entomopathog ...200817662996
antifungal activity of chitinases from trichoderma aureoviride dy-59 and rhizopus microsporus vs-9.two chitinolytic fungal strains, trichoderma aureoviride dy-59 and rhizopus microsporus vs-9, were isolated from soil samples of korea and vietnam, respectively. dy-59 and vs-9 crude chitinases secreted by these fungi in the 0.5% swollen chitin culture medium had an optimal ph of 4 and the optimal temperatures of 40 degrees c and 60 degrees c, respectively. enzymatic hydrolysis products from crab swollen chitin were n-acetyl-beta-d-glucosamine (glcnac) by dy-59 chitinase, and glcnac and n, n'-di ...200817896135
antifungal efficacy of voriconazole, itraconazole and amphotericin b in experimental fusarium solani keratitis.to evaluate the efficacy of topical voriconazole in an experimental rabbit model of fusarium keratitis.200817912543
sewage and community shower drains are environmental reservoirs of fusarium solani species complex group 1, a human and plant pathogen.in two recent studies, clinical isolates in the fusarium solani species complex (fssc) were sequenced; one of the most common lineages was fssc group 1 (fssc 1), a phylogenetic species that is synonymous with f. solani f. sp. cucurbitae race 2, a pathogen of cucurbit fruits. fssc 1 was also identified in sink and shower drains in two hospitals. the environmental sources of fssc 1 are important for understanding the epidemiology of both human and plant diseases caused by this organism. fssc 1 was ...200817916080
comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis.the activity of various lipases was compared, in both free and immobilized forms, using the kinetics of the hydrolysis reaction of p-nitrophenyl butyrate, which was followed with in situ uv/vis diode array spectrophotometry. several enzymes were used to catalyze the reaction, namely candida antarctica lipase b and fusarium solani pisi cutinase wildtype and three single-mutation variants. the enzymes were tested in three different forms: free, immobilized as cross-linked aggregates and supported ...200817940805
rational design of a new one-step purification strategy for candida antarctica lipase b by ion-exchange chromatography.a fast and efficient one-step method for purification of lipase b from candida antarctica by ion-exchange chromatography was developed by rational design. the electrostatic properties of the enzyme were calculated and validated by isoelectric focusing and measurement of the titration curve. c. antarctica lipase b shows an unusual ph profile with a broad isoelectric region from ph 4 to 8. at ph 3 c. antarctica lipase b can be bound to a cation-exchange chromatography column and was purified to ho ...200818154980
dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ...200818160452
occupational hypersensitivity pneumonitis due to molds in an onion and potato sorter.a 42-year-old female non-smoking onion and potato sorter developed work-related shortness of breath, cough, fatigue and flu-like symptoms. the diagnosis of hypersensitivity pneumonitis was based on patchy infiltrates in both lungs on high resolution computed tomography and lymphocytosis of 71% in a bronchoalveolar lavage sample with a cd4/cd8 ratio of 0.4. exposure cessation and initial corticosteroid therapy resulted in complete recovery. igg antibodies to penicillium species and fusarium solan ...200818161826
interactions between glyphosate and autochthonous soil fungi surviving in aqueous solution of glyphosate.the survival of autochthonous fungi in soil treated with 1mm aqueous solution of glyphosate was investigated. significant differences in the total number of fungi in the studied objects were observed, and additionally significant qualitative changes were encountered. the dominating group of fungi belonged to genus fusarium: fusarium solani h30, fusarium solani h50 and fusarium oxysporum h80. interactions between the isolated strains of fungi and varying concentrations of glyphosate were determin ...200818177917
activities of voriconazole, itraconazole and amphotericin b in vitro against 590 moulds from 323 patients in the voriconazole phase iii clinical studies.fungal pathogens from the voriconazole trials were identified and tested for susceptibility at two reference laboratories.200818222957
fungal root endophytes from natural vegetation in mediterranean environments with special reference to fusarium spp.surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal and inland soils, both sandy soils and salt marshes) under either water or salt stress in the alicante province (southeast spain). all plant species examined were colonized by endophytic fungi. a total of 1830 fungal isolates were obtained and identified by morphological and molecular [internal transcribed spacer (its) and translation elongation factor-1alpha gene region (tef ...200818248439
antifungal susceptibility profile of clinical fusarium spp. isolates identified by molecular methods.to analyse the susceptibility pattern of a collection of fusarium clinical isolates.200818263569
protein structure and dynamics in ionic liquids. insights from molecular dynamics simulation studies.we present in this work the first molecular simulation study of an enzyme, the serine protease cutinase from fusarium solani pisi, in two ionic liquids (ils): 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][pf6]) and 1-butyl-3-methylimidazolium nitrate ([bmim][no(3)]). we tested different water contents in these ils at room temperature (298 k) and high temperature (343 k), and we observe that the enzyme structure is highly dependent on the amount of water present in the il media. we show ...200818266354
amphotericin b formulations variably enhance antifungal activity of human neutrophils and monocytes against fusarium solani: comparison with aspergillus fumigatus.lipid formulations of amphotericin b (ambf) are widely used in the treatment of life-threatening infections caused by aspergillus fumigatus and fusarium solani. we aimed to compare the immunomodulatory effects of four ambf, deoxycholate (damb), liposomal (lamb), lipid complex (ablc) and colloidal dispersion (abcd), on the oxidative antifungal activities of human neutrophils (pmns) and monocytes (mncs) against hyphae of a. fumigatus and f. solani.200818272514
orientation of cutinase adsorbed onto pmma nanoparticles probed by tryptophan fluorescence.the fluorescence of the single tryptophan (trp69) of cutinase from fusarium solani pisi, free in aqueous solution and adsorbed onto the surface of poly(methyl methacrylate) (pmma) latex particles, was studied at phs of 4.5 and 8.0. the monodisperse pmma particles (d=106.0+/-0.1 nm) were coated with a quite compact monolayer of cutinase at both ph values. the trp decay curve of the folded free cutinase in solution can only be fitted with a sum of four exponentials with lifetimes of 0.05, 0.3-0.4, ...200818311968
expression in escherichia coli, purification, refolding and antifungal activity of an osmotin from solanum nigrum.abstract:200818334031
[non-dermatophytic moulds: onychomycosis in four patients infected with the human immunodeficiency virus].patients infected with human immunodeficiency virus (hiv) are a risk group for onychomycosis, fungal infections caused mainly by dermatophytes and yeast. however, non-dermatohytic moulds are becoming common agents for nail infections in this population of patients. we report four cases of onychomycosis caused by non-dermatophytic moulds (aspergillus niger, scytalidium hyalinum, scytalidium dimidiatum and fusarium solani) in patients infected with hiv from recife, pernambuco, brazil. onychomicosi ...200818338928
antifungal and antibacterial activities of taxus wallichiana zucc.current study was undertaken to evaluate the in vitro antifungal and antibacterial potential of methanol extract and subsequent fractions obtained after partitioning in organic solvents with variable polarity of the aerial parts of the tree taxus wallichiana zucc. traditionally, this plant is often used in folk medicines in pakistan for treating microbial infections. in order to rationalize the traditional use, methanol extracts of leaf, bark, and heartwood of taxus wallichiana zucc. were tested ...200818343912
fungal removal of gaseous hexane in biofilters packed with poly(ethylene carbonate) pine sawdust or peat composites.the removal of volatile organic compounds (voc) in biofilters packed with organic filter beds, such as peat moss (pm) and pine sawdust (ps), frequently presents drawbacks associated to the collapse of internal structures affecting the long-term operation. poly(ethylene ether carbonate) (peec) groups grafted to these organic carriers cross linked with 4,4'-methylenebis(phenylisocyanate) (mdi) permitted fiber aggregation into specific shapes and with excellent hexane sorption performance. modified ...200818351679
[diversity of facultatively anaerobic microscopic mycelial fungi in soils].the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ...200818365728
fusarium solani in the post-transplant patient: an unusual fungus.the incidence of fungal infections in post-transplant patients ranges from 2% to 50%, and more uncommon pathogens are being isolated. earlier, the fungi seen were candida, aspergillus, and cryptococcus; and of late, histoplasma, coccidiodomycoses, and dematiaceous fungi are seen. in this article, a patient with invasive fusariosis is presented, and literature of this uncommon fungus is reviewed.200818372269
antifungal activity of coumarins.the antifungal activity of 40 coumarins was tested against the fungal strains: candida albicans (atcc 14053), aspergillus fumigatus (atcc 16913) and fusarium solani (atcc 36031), using the broth microdilution method. osthenol showed the most effective antifungal activity among all the compounds tested, with a mic value of 125 microg/ml for fusarium solani and 250 micro/ml for candida albicans and aspergillus fumigatus. the antifungal potential of this prenylated coumarin can be related to the pr ...200818386483
expression of toll-like receptors in the fusarium solani infected cornea.to investigate the expression of toll-like receptors (tlrs) in the human cornea with fusarium solani (f. solani) keratitis.200818398706
enzymatic hydrolysis of ptt polymers and oligomers.oligomers and polymers (film, fabrics) of the linear aromatic polyester poly(trimethylene terephthalate) (ptt) were treated with polyesterases from thermomyces lanuginosus, penicillium citrinum, thermobifida fusca and fusarium solani pisi. the cutinase from t. fusca was found to release the highest amounts of hydrolysis products from ptt materials and was able to open and hydrolyse a cyclic ptt dimer according to rp-hplc-uv detection. in contrast, the lipase from t. lanuginosus also showed activ ...200818405994
improving activity and stability of cutinase towards the anionic detergent aot by complete saturation mutagenesis.cutinase is an enzyme suitable for detergent applications as well as for organic synthesis in non-aqueous solvents. however, its inactivation in the presence of anionic surfactants is a problem which we have addressed by creating a complete saturation library. for this, the cutinase gene from fusarium solani pisi was mutated to incorporate all 19 possible amino acid exchanges at each of the 214 amino acid positions. the resulting library was screened for active variants with improved stability i ...200818424821
hypotin, a novel antipathogenic and antiproliferative protein from peanuts with a sequence similar to those of chitinase precursors.a protein designated hypotin, with both antifungal and antibacterial activity, was isolated from peanut (arachis hypogaea) seeds. the isolation procedure included extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, ion chromatography, and gel filtration. the protein exhibited a molecular mass of 30.4 kda in sds-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions, indicating that it is a monomeric protein. its n-terminal sequence ...200717979231
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