Publications

role of t3ss-1 sipd protein in protecting mice against non-typhoidal salmonella typhimurium.	salmonella enterica species are enteric pathogens that cause severe diseases ranging from self-limiting gastroenteritis to enteric fever and sepsis in humans. these infectious diseases are still the major cause of morbidity and mortality in low-income countries, especially in children younger than 5 years and immunocompromised adults. vaccines targeting typhoidal diseases are already marketed, but none protect against non-typhoidal salmonella. the existence of multiple non-typhoidal salmonella s ...	2016	27992422
salmonella typhimurium undergoes distinct genetic adaption during chronic infections of mice.	typhoid fever caused by salmonella enterica serovar typhi (s. typhi) is a severe systemic human disease and endemic in regions of the world with poor drinking water quality and sewage treatment facilities. a significant number of patients become asymptomatic life-long carriers of s. typhi and serve as the reservoir for the disease. the specific mechanisms and adaptive strategies enabling s. typhi to survive inside the host for extended periods are incompletely understood. yet, elucidation of the ...	2016	26955808
study of salmonella typhimurium infection in laying hens.	members of salmonella enterica are frequently involved in egg and egg product related human food poisoning outbreaks worldwide. in australia, salmonella typhimurium is frequently involved in egg and egg product related foodborne illness and salmonella mbandaka has also been found to be a contaminant of the layer farm environment. the ability possessed by salmonella enteritidis to colonize reproductive organs and contaminate developing eggs has been well-described. however, there are few studies ...	2016	26941727
strain-specific survival of salmonella enterica in peanut oil, peanut shell, and chia seeds.	in north america, outbreaks of salmonella have been linked to low-water activity (aw) foods, such as nuts and seeds. these outbreaks have implicated an assortment of salmonella serotypes. some salmonella serotypes (e.g., enteritidis and typhimurium) cause high proportions of salmonellosis. nevertheless, there has recently been an emergence of uncommon salmonella serotypes and strains (e.g., tennessee, hartford, and thompson) in low-aw foods. the aim of this study was to evaluate the survival cha ...	2016	26939645
compensating the fitness costs of synonymous mutations.	synonymous mutations do not change the sequence of the polypeptide but they may still influence fitness. we investigated in salmonella enterica how four synonymous mutations in the rpst gene (encoding ribosomal protein s20) reduce fitness (i.e., growth rate) and the mechanisms by which this cost can be genetically compensated. the reduced growth rates of the synonymous mutants were correlated with reduced levels of the rpst transcript and s20 protein. in an adaptive evolution experiment, these f ...	2016	26882986
the horizontally-acquired response regulator ssrb drives a salmonella lifestyle switch by relieving biofilm silencing.	a common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. two major serovars of salmonella enterica, typhi and typhimurium, have evolved a two-component regulatory system to exist inside salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. here we present evidence that ssrb, a transcriptional regulator encoded on the spi-2 pathogenicity ...	2016	26880544
prevalence of nontyphoidal salmonella and salmonella strains with conjugative antimicrobial-resistant serovars contaminating animal feed in texas.	the objective of this study was to characterize 365 nontyphoidal salmonella enterica isolates from animal feed. among the 365 isolates, 78 serovars were identified. twenty-four isolates (7.0%) were recovered from three of six medicated feed types. three of these isolates derived from the medicated feed, salmonella newport, salmonella typhimurium var. o 5- (copenhagen), and salmonella lexington var. 15+ (manila), displayed antimicrobial resistance. susceptibility testing revealed that only 3.0% ( ...	2016	26818979
characterization and antimicrobial resistance of salmonella typhimurium isolates from clinically diseased pigs in korea.	this study investigated the prevalence of salmonella enterica serovar and antimicrobial resistance in salmonella typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in korea. isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. among 94 salmonella isolates, 81 (86.2%) were identified as being of the salmonella typhimurium serotype, followed by salmonella derby (6 of 94, 6.4%), salmon ...	2016	28221916
population dynamics analysis of ciprofloxacin-persistent s. typhimurium cells in a mouse model for salmonella diarrhea.	in vivo, antibiotics are often surprisingly inefficient at eliminating bacterial pathogens. in the case of ciprofloxacin therapy in a salmonella enterica subspecies 1 serovar typhimurium (s. typhimurium, s. tm) mouse infection model, this has been traced to tolerant bacterial cells surviving in lymph node monocytes (i.e., classical dendritic cells). to analyze the growth characteristics of these persisters, we have developed a population dynamics approach using mixtures of wild-type isogenic tag ...	2016	26468110
salmonella enteritidis effector avra stabilizes intestinal tight junctions via the jnk pathway.	salmonella pathogenesis studies to date have focused on salmonella typhimurium, and the pathogenesis of a second major serotype, salmonella enteritidis, is poorly understood. salmonella spp. possess effector proteins that display biochemical activities and modulate host functions. here, we generated a deletion mutant of the effector avra, s.e-avra(-), and a plasmid-mediated complementary strain, s.e-avra(-)/pavra(+) (s.e-avra(+)), in s. enteritidis. using in vitro and in vivo infection models, w ...	2016	27875307
minimal processing of iceberg lettuce has no substantial influence on the survival, attachment and internalization of e. coli o157 and salmonella.	the influence of a selection of minimal processing techniques (sanitizing wash prior to packaging, modified atmosphere, storage conditions under light or in the dark) was investigated in relation to the survival of, attachment to and internalization of enteric pathogens in fresh produce. cut iceberg lettuce was chosen as a model for fresh produce, escherichia coli o157:h7 (e. coli o157) and salmonella enterica were chosen as pathogen models. care was taken to simulate industrial post-harvest pro ...	2016	27591385
identification of potential therapeutics to conquer drug resistance in salmonella typhimurium: drug repurposing strategy.	salmonella typhimurium is the main cause of gastrointestinal illness in humans, and treatment options are decreasing because drug-resistant strains have emerged.	2016	27761807
the effect of phytogenic feed additives to substitute in-feed antibiotics on growth traits and blood biochemical parameters in broiler chicks challenged with salmonella typhimurium.	there is a growing concern over the use of antibiotics due to the increased resistance of pathogens in broiler. the present study was designed to find the comparative effect of an antibiotic, and some phytogenic on performance traits, blood biochemical parameters, and antioxidant status during starter phase exposed to salmonella typhimurium challenge. a total of 560-day-old broiler chicks (ross 308) were randomly allocated to seven treatments (eight replicates). control (basal diet); t1, infecte ...	2016	27646442
wgs for surveillance of antimicrobial resistance: a pilot study to detect the prevalence and mechanism of resistance to azithromycin in a uk population of non-typhoidal salmonella.	wgs and phenotypic methods were used to determine the prevalence of azithromycin resistance in salmonella enterica isolates from the uk and to identify the underlying mechanisms of resistance.	2016	27585964
outer membrane vesicles derived from salmonella typhimurium mutants with truncated lps induce cross-protective immune responses against infection of salmonella enterica serovars in the mouse model.	salmonella enterica cause diarrheal and systemic diseases and are of considerable concern worldwide. vaccines that are cross-protective against multiple serovars could provide effective control of salmonella-mediated diseases. bacteria-derived outer membrane vesicles (omvs) are highly immunogenic and are capable of eliciting protective immune responses. alterations in lipopolysaccharide (lps) length can result in outer membrane remodeling and composition of outer membrane proteins (omps) changin ...	2016	27578609
[investigation of antitumorigenic effects of food-borne non-pathogenic and pathogenic salmonella enterica strains on mef, du145 and hela cell lines].	basic applications in cancer therapy may fail to eradicate cancer cells completely, they can show toxic affects to healthy cells and development of resistance to antitumor agents may increase tendency to metastasis. bacterial therapies have the advantage of specific targetting of tumors by selective toxicity, responsiveness to external signals, self-propelling capacity, and the sense of microenvironment. the most interest on the bacterial cancer therapy is about salmonella spp. with a special em ...	2016	27525394
mapping b-cell responses to salmonella enterica serovars typhimurium and enteritidis in chickens for the discrimination of infected from vaccinated animals.	serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. however, the compatibility of these strategies is limited by a lack of assays capable of differentiating infected from vaccinated animals (diva tests) for established killed or attenuated vaccines. here, we used next generation phage-display (ngpd) and a 2-proportion z score analysis to identify peptides that were preferentially bound by igy from chickens infected wit ...	2016	27510219
salmonella infection in healthy pet reptiles: bacteriological isolation and study of some pathogenic characters.	the fecal samples from 213 captive reptiles were examined, and 29 (13.61%) salmonella enterica isolates were detected: 14/62 (22.58%) from chelonians, 14/135 (10.37%) from saurians, and 1/16 (6.25%) from ophidians. the isolates were distributed among 14 different serotypes: miami, ebrie, hermannsweder, tiergarten, tornov, pomona, poona, goteborg, abaetetube, nyanza, kumasi, typhimurium, 50:b:z6, 9,12:z29:1,5, and a non-motile serotype with antigenic formula 1,4,[5],12:-:-. salmonella typhimurium ...	2016	27352973
invasive infections with nontyphoidal salmonella in sub-saharan africa.	invasive nontyphoidal salmonella (nts) infections in africa cause an enormous burden of illness. these infections are often devastating, with mortality estimated at 20%, even with appropriate antimicrobial therapy. two major groups-young children and hiv-infected adults-suffer the great majority of these infections. in children, younger age itself, as well as malaria, malnutrition, and hiv infection, are prominent risk factors. in adults, hiv infection is by far the most important risk factor. t ...	2016	27337467
antimelanoma effect of salmonella typhimurium integration host factor mutant in murine model.	this study aimed to evaluate an attenuated salmonella ihfa-null mutant strain as therapeutic agent to control tumor growth.	2016	27328776
serotype epidemiology and multidrug resistance patterns of salmonella enterica infecting humans in italy.	salmonella enterica is the zoonotic agent most frequently responsible for foodborne infections in humans worldwide. in this work the presence of s. enterica was investigated in 734 unique enteropathogenic isolates collected from human patients between 2011 and 2012.	2016	27252785
tiny turtles purchased at pet stores are a potential high risk for salmonella human infection in the valencian region, eastern spain.	turtles may be considered unsafe pets, particularly in households with children. this study aimed to assess salmonella carriage by turtles in pet stores and in private ownership to inform the public of the potential health risk, enabling informed choices around pet selection. during the period between september and october 2013, 24 pet stores and 96 private owners were sampled in the valencian region (eastern spain). salmonella identification procedure was based on iso 6579: 2002 recommendations ...	2016	27228194
house sparrows do not constitute a significant salmonella typhimurium reservoir across urban gradients in flanders, belgium.	in recent decades major declines in urban house sparrow (passer domesticus) populations have been observed in north-western european cities, whereas suburban and rural house sparrow populations have remained relatively stable or are recovering from previous declines. differential exposure to avian pathogens known to cause epidemics in house sparrows may in part explain this spatial pattern of declines. here we investigate the potential effect of urbanization on the development of a bacterial pat ...	2016	27168186
genetic relatedness of salmonella serovars isolated from catfish (clarias gariepinus) and tilapia (tilapia mossambica) obtained from wet markets and ponds in penang, malaysia.	a total of 43 salmonella enterica isolates belonging to different serovars (salmonella albany, salmonella agona, salmonella corvallis, salmonella stanley, salmonella typhimurium, salmonella mikawasima, and salmonella bovismorbificans) were isolated from catfish (clarias gariepinus) and tilapia (tilapia mossambica) obtained from nine wet markets and eight ponds in penang, malaysia. thirteen, 19, and 11 isolates were isolated from 9 of 32 catfish, 14 of 32 tilapia, and 11 of 44 water samples, resp ...	2016	27052872
immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice.	vaccination is the most effective method for preventing rabies virus (rabv) infection in both humans and animals; however, no satisfactory vaccine has been developed for use worldwide. in the present study, we investigated the immunoadjuvant properties of salmonella typhimurium flagellin (fljb, flic, and fljb'-flic) to improve immune responses against the rabies vaccine (rv) and the protective efficacy of the whole-killed rabies vaccine (wkrv) with or without flagellins in balb/c mice. we also c ...	2016	26650039
the bacterial two-hybrid system uncovers the involvement of acetylation in regulating of lrp activity in salmonella typhimurium.	n(𝜀)-lysine acetylation is an abundant and important post-translational modification in bacteria. we used the bacterial two-hybrid system to screen the genome library of the salmonella typhimurium to identify potential proteins involved in acetyltransferase pat - or deacetylase cobb-mediated acetylation. then, the in vitro (de)acetylation assays were used to validate the potential targets, such as stm14_1074, nrdf, rhar. lrp, a leucine-responsive regulatory protein and global regulator, was sho ...	2016	27909434
reduction of salmonella typhimurium by fermentation metabolites of diamond v original xpc in an in vitro anaerobic mixed chicken cecal culture.	fermentation metabolites of diamond v original xpc™ (xpc), a biological product derived from yeast fermentation, were evaluated for their ability to reduce the salmonella typhimurium population using an in vitro mixed anaerobic culture system containing cecal microbiota to simulate chicken hindgut conditions. four different samples were prepared: anaerobic mixed culture containing (1) feed only, (2) cecal only (ceca were harvested from 42 days old broiler chickens), (3) feed and cecal contents, ...	2016	27695699
cytotoxic, mutagenicity, and genotoxicity effects of guanylhydrazone derivatives.	several studies have reported that guanylhydrazones display a variety of desirable biological properties, such as antihypertensive, antibacterial, and antimalarial behaviour. they furthermore promote anti-pneumocystosis and anti-trypanosomiasis, exhibit antitumor activity, and show significant cytotoxicity against cancer cell lines. in this work, we have evaluated the cytotoxicity, mutagenicity, and genotoxicity of two guanylhydrazones derivatives, (e)-2-[(2,3-dimethoxyphenyl) methylene] hydrazi ...	2016	27476330
designing, construction and characterization of genetically encoded fret-based nanosensor for real time monitoring of lysine flux in living cells.	engineering microorganisms in order to improve the metabolite flux needs a detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo. fluorescence resonance energy transfer (fret) based genetically encoded nanosensors represent a promising tool for measuring the metabolite levels and corresponding rate changes in live cells. here, we report the development of a series of fret based genetically encoded nanosensor for real time measurement of lysine ...	2016	27334743
dna protection against oxidative damage using the hydroalcoholic extract of garcinia mangostana and alpha-mangostin.	garcinia mangostana, popularly known as "mangosteen fruit," originates from southeast asia and came to brazil about 80 years ago where it mainly grows in the states of pará and bahia. although mangosteen or its extracts have been used for ages in asian folk medicine, data on its potential genotoxicity is missing. we, therefore, evaluated genotoxicity/mutagenicity of hydroethanolic mangosteen extract [hegm, 10 to 640 μg/ml] in established test assays (comet assay, micronucleus test, and salmonell ...	2016	27042187
interactions between sanitizers and packaging gas compositions and their effects on the safety and quality of fresh-cut onions (allium cepa l.).	onions are one of the most widely utilized vegetables worldwide, with demand for fresh-cut onions steadily increasing. due to heightened safety concerns and consumer demand, the implications of sanitizing and packaging on fresh-cut onion safety and quality need to be better understood. the objective of this study was to investigate the effect of produce sanitizers, in-package atmospheres, and their interactions on the growth of salmonella typhimurium, mesophilic aerobic bacteria, yeast and mold, ...	2016	26656528
heterologous pseudomonas aeruginosa o-antigen delivery using a salmonella enterica serovar typhimurium weca mutant strain.	there is a broad interest in adapting live vaccine strains (lvs) for use as recombinant vaccines that can deliver heterologous antigens. the salmonella enterica serovar typhimurium sl1344 δweca lvs contains a mutation in weca that abrogates production of enterobacterial common antigen. this δweca strain is attenuated in vivo, persistently colonizes the host, and protects against both wild type and cross-salmonella serovar lethal challenge in a murine model of salmonellosis. given these character ...	2016	27476047
a systems biology analysis unfolds the molecular pathways and networks of two proteobacteria in spaceflight and simulated microgravity conditions.	bacteria are important organisms for space missions due to their increased pathogenesis in microgravity that poses risks to the health of astronauts and for projected synthetic biology applications at the space station. we understand little about the effect, at the molecular systems level, of microgravity on bacteria, despite their significant incidence. in this study, we proposed a systems biology pipeline and performed an analysis on published gene expression data sets from multiple seminal st ...	2016	27623197
optimizing bacteriophage surface densities for bacterial capture and sensing in quartz crystal microbalance with dissipation monitoring.	surface immobilized bacteriophages (phages) are increasingly used as biorecognition elements on bacterial biosensors (e.g., on acoustical, electrical, or optical platforms). the phage surface density is a critical factor determining a sensor's bacterial binding efficiencies; in fact, phage surface densities that are too low or too high can result in significantly reduced bacterial binding capacities. identifying an optimum phage surface density is thus crucial when exploiting the bacteriophages' ...	2016	27171886
rapid screening of waterborne pathogens using phage-mediated separation coupled with real-time pcr detection.	escherichia coli o157:h7 is a ubiquitous pathogen which can be linked to foodborne outbreaks worldwide. in addition to the significant illnesses, hospitalizations, and deaths resulting from the outbreaks, there can be severe economic consequences to farmers, food manufacturers, and municipalities. a rapid detection assay which can validate sanitation and water quality would prove beneficial to these situations. here, we report a novel bacteriophage-mediated detection of e. coli o157:h7 which uti ...	2016	27071764
genetic control of bacterial biofilms.	nearly all bacterial species, including pathogens, have the ability to form biofilms. biofilms are defined as structured ecosystems in which microbes are attached to surfaces and embedded in a matrix composed of polysaccharides, edna, and proteins, and their development is a multistep process. bacterial biofilms constitute a large medical problem due to their extremely high resistance to various types of therapeutics, including conventional antibiotics. several environmental and genetic signals ...	2016	26294280
the isolation and identification of pantoea dispersa strain jfs as a non-pathogenic surrogate for salmonella typhimurium phage type 42 in flour.	salmonella is a common pathogen which has been the cause of foodborne illness outbreaks implicating a variety of commodities, including low-moisture foods such as flour. salmonella costs more than any other pathogen in the united states in terms of health care expenses and time of lost work. heat treatment can be used to reduce salmonella and other pathogens in flour to safe levels. however, in low-moisture foods, process times must be increased to achieve adequate lethality, possibly resulting ...	2016	26686596
microbiological, clinical and molecular findings of non-typhoidal salmonella bloodstream infections associated with malaria, oriental province, democratic republic of the congo.	in sub-saharan africa, non-typhoidal salmonella (nts) can cause bloodstream infections, referred to as invasive non-typhoidal salmonella disease (ints disease); it can occur in outbreaks and is often preceded by malaria. data from central africa is limited.	2016	27286886
fr-900098, an antimalarial development candidate that inhibits the non-mevalonate isoprenoid biosynthesis pathway, shows no evidence of acute toxicity and genotoxicity.	fr-900098 is an inhibitor of 1-deoxy-d-xylulose-5-phosphate (dxp) reductoisomerase, the second enzyme in the non-mevalonate isoprenoid biosynthesis pathway. in previous studies, fr-900098 was shown to possess potent antimalarial activity in vitro and in a murine malaria model. in order to provide a basis for further preclinical and clinical development, we studied the acute toxicity and genotoxicity of fr-900098. we observed no acute toxicity in rats, i.e. there were no clinical signs of toxicit ...	2016	27260413
the relationship between invasive nontyphoidal salmonella disease, other bacterial bloodstream infections, and malaria in sub-saharan africa.	country-specific studies in africa have indicated that plasmodium falciparum is associated with invasive nontyphoidal salmonella (ints) disease. we conducted a multicenter study in 13 sites in burkina faso, ethiopia, ghana, guinea-bissau, kenya, madagascar, senegal, south africa, sudan, and tanzania to investigate the relationship between the occurrence of ints disease, other systemic bacterial infections, and malaria.	2016	26933016
construction of a bivalent dna vaccine co-expressing s genes of transmissible gastroenteritis virus and porcine epidemic diarrhea virus delivered by attenuated salmonella typhimurium.	porcine transmissible gastroenteritis virus (tgev) and porcine epidemic diarrhea virus (pedv) can cause severe diarrhea in newborn piglets and led to significant economic losses. the s proteins are the main structural proteins of pedv and tgev capable of inducing neutralizing antibodies in vivo. in this study, a dna vaccine sl7207 (pvaxd-ps1-ts) co-expressing s proteins of tgev and pedv delivered by attenuated salmonella typhimurium was constructed and its immunogenicity in piglets was investiga ...	2016	26980672
influence of water activity on inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in peanut butter by microwave heating.	this study evaluated the efficacy of a 915 mhz microwave with 3 different electric power levels to inactivate three pathogens in peanut butter with different aw. peanut butter inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes (0.3, 0.4, and 0.5 aw) were treated with a 915 mhz microwave with 2, 4, and 6 kw for up to 5 min. six kw 915 mhz microwave treatment for 5 min reduced these three pathogens by 1.97 to >5.17 log cfu/g. four kw 915 mh ...	2016	27554151
achillea schurii flowers: chemical, antioxidant, and antimicrobial investigations.	this study aims to evaluate the phenolic profile, and antioxidant and antimicrobial activity of achillea schurii sch.-bip., an endemic species from romania that has not been investigated yet. the chromatographic profile of the phenolic components was obtained using the hplc-ms method, while the total polyphenol, flavonoid, caffeic acid derivative contents were quantified using spectrophotometric methods. the antioxidant activity was evaluated using different methods: dpph radical scavenging, hem ...	2016	27529204
a piezoelectric immunosensor for specific capture and enrichment of viable pathogens by quartz crystal microbalance sensor, followed by detection with antibody-functionalized gold nanoparticles.	a sensitive bacteria enrichment and detection system for viable escherichia coli o157:h7 was developed using a piezoelectric biosensor-quartz crystal microbalance (qcm) with antibody-functionalized gold nanoparticles (aunps) used as detection verifiers and amplifiers. in the circulating-flow qcm system, capture antibodies for e. coli o157:h7 were first immobilized onto the qcm chip. the sample containing e. coli o157:h7 was circulated through the system in the presence of 10 ml of brain heart in ...	2016	22683250
transcriptomic approaches in the zebrafish model for tuberculosis-insights into host- and pathogen-specific determinants of the innate immune response.	mycobacterium marinum infection in zebrafish has become a well-established model of tuberculosis. both embryonic and adult zebrafish infection studies have contributed to our knowledge of the development and function of tuberculous granulomas, which are typical of mycobacterial pathogenesis. in this review we discuss how transcriptome profiling studies have helped to characterize this infection process. we illustrate this using new rna sequencing (rna-seq) data that reveals three main phases in ...	2016	27503359
in vitro evaluation of the cyto-genotoxic potential of ruthenium(ii) scar complexes: a promising class of antituberculosis agents.	tuberculosis is a top infectious disease killer worldwide, caused by the bacteria mycobacterium tuberculosis. increasing incidences of multiple drug-resistance (mdr) strains are emerging as one of the major public health threats. however, the drugs in use are still incapable of controlling the appalling upsurge of mdr. in recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted mdr-tub ...	2016	26994489
contribution of increased isg15, isgylation and deregulated type i ifn signaling in usp18 mutant mice during the course of bacterial infections.	host genetics has a key role in susceptibility to salmonella typhimurium infection. we previously used n-ethyl-n-nitrosourea (enu) mutagenesis to identify a loss-of-function mutation within the gene ubiquitin-specific peptidase 18 (usp18(ity9)), which confers increased susceptibility to salmonella typhimurium. usp18 functions to regulate type i interferon (ifn) signaling and as a protease to remove isg15 from substrate proteins. usp18(ity9) mice are susceptible to infection with salmonella typhi ...	2016	24807690
transfer of pathogens from cantaloupe rind to preparation surfaces and edible tissue as a function of cutting method.	whole and cut cantaloupes have been implicated as vehicles in foodborne illness outbreaks of norovirus, salmonellosis, and listeriosis. preparation methods that minimize pathogen transfer from external surfaces to the edible tissue are needed. two preparation methods were compared for the transfer of listeria monocytogenes, salmonella enterica serovar typhimurium lt2, murine norovirus, and tulane virus from inoculated cantaloupe rinds to edible tissue and preparation surfaces. for the first meth ...	2016	27296423
fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device.	this work describes fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device, which is called a lab-on-a-disc. all the processes for molecular diagnostics including dna extraction and purification, dna amplification and amplicon detection were integrated on a single disc. silica microbeads incorporated in the disc enabled extraction and purification of bacterial genomic dna from bacteria-contaminated milk samples. we targeted four kinds of fo ...	2016	27112702
efficacy of uv-c irradiation for inactivation of food-borne pathogens on sliced cheese packaged with different types and thicknesses of plastic films.	in this study, the efficacy of using uv-c light to inactivate sliced cheese inoculated with escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes and, packaged with 0.07 mm films of polyethylene terephthalate (pet), polyvinylchloride (pvc), polypropylene (pp), and polyethylene (pe) was investigated. the results show that compared with pet and pvc, pp and pe films showed significantly reduced levels of the three pathogens compared to inoculated but non-treated controls. the ...	2016	27052716
evaluation of a cross contamination model describing transfer of salmonella spp. and listeria monocytogenes during grinding of pork and beef.	in a previous study, a model was developed to describe the transfer and survival of salmonella during grinding of pork (møller, c.o.a., nauta, m.j., christensen, b.b., dalgaard, p., hansen, t.b., 2012. modelling transfer of salmonella typhimurium dt104 during simulation of grinding of pork. journal of applied microbiology 112 (1), 90-98). the robustness of this model is now evaluated by studying its performance for predicting the transfer and survival of salmonella spp. and listeria monocytogene ...	2016	27035678
antibacterial activity of aquatic gliding bacteria.	the study aimed to screen and isolate strains of freshwater aquatic gliding bacteria, and to investigate their antibacterial activity against seven common pathogenic bacteria. submerged specimens were collected and isolated for aquatic gliding bacteria using four different isolation media (dw, ma, sap2, and vy/2). gliding bacteria identification was performed by 16s rrna gene sequencing and phylogenetic analysis. crude extracts were obtained by methanol extraction. antibacterial activity against ...	2016	26885469
structural and biochemical insights into the peptidoglycan hydrolase domain of flgj from salmonella typhimurium.	flgj is a glycoside hydrolase (gh) enzyme belonging to the carbohydrate active enzyme (cazy) family gh73. it facilitates passage of the bacterial flagellum through the peptidoglycan (pg) layer by cleaving the β-1,4 glycosidic bond between n-acetylglucosamine and n-acetylmuramic acid sugars that comprise the glycan strands of pg. here we describe the crystal structure of the gh domain of flgj from bacterial pathogen salmonella typhimurium (stflgj). interestingly, the active site of stflgj was blo ...	2016	26871950
phenolic compounds, antioxidant, and antibacterial properties of pomace extracts from four virginia-grown grape varieties.	grape pomace is a potential source of natural antioxidant and antimicrobial agents. phenolic compounds, antioxidant, and antibacterial properties of pomace extracts from four virginia-grown grape varieties were investigated. white grape pomaces had higher (p < 0.05) solvent extraction yield than red varieties. concentrations of total phenolic (tpc), total flavonoid (tfc), total anthocyanin (tac), tannins, condensed tannins (ct), as well as antioxidant capacities (dpph• and abts•+free radical sca ...	2016	26788319
effectiveness of superheated steam for inactivation of escherichia coli o157:h7, salmonella typhimurium, salmonella enteritidis phage type 30, and listeria monocytogenes on almonds and pistachios.	this study was undertaken to evaluate the effectiveness of superheated steam (shs) on the inactivation of escherichia coli o157:h7, salmonella typhimurium, salmonella enteritidis phage type (pt) 30 and listeria monocytogenes on almonds and in-shell pistachios and to determine the effect of superheated steam heating on quality by measuring color and texture changes. almonds and in-shell pistachios inoculated with four foodborne pathogens were treated with saturated steam (ss) at 100 °c and shs at ...	2016	26773253
effect of sanitizer combined with steam heating on the inactivation of foodborne pathogens in a biofilm on stainless steel.	the combined effect of chemical sanitizers including sodium hypochlorite, hydrogen peroxide, iodophor, and benzalkonium chloride with steam heating on the inactivation of biofilms formed by escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on stainless steel was investigated. six day old biofilms, comprised of a mixture of three strains each of three foodborne pathogens, were produced on stainless steel coupons at 25 °c and treated with each sanitizer alone (for 5, 15, ...	2016	26742615
inactivation kinetics of listeria monocytogenes and salmonella enterica serovar typhimurium on fresh-cut bell pepper treated with slightly acidic electrolyzed water combined with ultrasound and mild heat.	the goal of this study was to enhance the antimicrobial effect of slightly acidic electrolyzed water (saew) through addition of synergistic treatment with ultrasound (us) and mild heat treatment in order to improve the microbial safety of fresh-cut bell pepper. to evaluate the synergistic effects, the weibull model was used to mathematically measure the effectiveness of the individual and combined treatments against listeria monocytogenes and salmonella typhimurium on the pepper. the combined tr ...	2016	26678144
survival of foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce.	knowing the survival characteristics of foodborne pathogens in raw ready-to-eat (rte) seafood is the key to predicting whether they pose a microbiological hazard. the present study examined the survival of escherichia coli o157:h7, salmonella typhimurium, vibrio parahaemoliticus, listeria monocytogenes, and staphylococcus aureus in raw rte crab marinated in soy sauce. inoculated crabs (initial bacterial population=4.1-4.4logcfu/g) were immersed in soy sauce and then stored at refrigeration (5°c) ...	2016	27591386
widespread shortening of 3' untranslated regions and increased exon inclusion are evolutionarily conserved features of innate immune responses to infection.	the contribution of pre-mrna processing mechanisms to the regulation of immune responses remains poorly studied despite emerging examples of their role as regulators of immune defenses. we sought to investigate the role of mrna processing in the cellular responses of human macrophages to live bacterial infections. here, we used mrna sequencing to quantify gene expression and isoform abundances in primary macrophages from 60 individuals, before and after infection with listeria monocytogenes and ...	2016	27690314
effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste using 915-megahertz microwave and ohmic heating.	the effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste was investigated using 915-mhz microwave heating (mw) and ohmic heating (oh). heating uniformity, pathogen inactivation, and quality aspects were determined with 1.8-, 2.1-, 2.4-, and 3.0-kw mw and corresponding oh. ginafit was used to analyze pathogen inactivation. the heating uniformity of mw-treated samples was inferior to that of oh-treated samples at low ...	2016	28221946
effect of temperature and nutrient concentration on survival of foodborne pathogens in deciduous fruit processing environments for effective hygiene management.	temperature and good sanitation practices are important factors for controlling growth of microorganisms. fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. when foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. the aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°c) ...	2016	28221909
fate of pathogenic bacteria associated with production of pickled sausage by using a cold fill process.	preservation by pickling has been used for many years to extend the shelf life of various types of food products. by storing meat products in a brine solution containing an organic acid, salt, spices, as well as other preservatives, the ph of the product is reduced, thus increasing the safety and shelf life of the product. pickling may involve the use of heated brines to further add to the safety of the food product. when precooked, ready-to-eat (rte) sausages are pickled with a heated brine sol ...	2016	28221846
enhanced antimicrobial effect of organic acid washing against foodborne pathogens on broccoli by vacuum impregnation.	this study was undertaken to evaluate the effect of vacuum impregnation applied to the washing process for removal of salmonella typhimurium and listeria monocytogenes from broccoli surfaces. broccoli was inoculated with the two foodborne pathogens and treated with simple dipping washing or with vacuum impregnation in 2% malic acid for 5, 10, 20, or 30 min. there were two methods of vacuum impregnation: continuous and intermittent. after 30 min of 101.3 kpa (=14.7 psi, simple dipping), 61.3 kpa ...	2016	26496412
synergism between carvacrol or thymol increases the antimicrobial efficacy of soy sauce with no sensory impact.	here, we examined the antimicrobial effects of soy sauce containing essential oils (eos) against escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes at 22°c and 4°c. to screen a variety of combined effects, soy sauce was mixed with six different eos (carvacrol, thymol, eugenol, trans-cinnamaldehyde, β-resorcylic acid, and vanillin), each at a concentration of 1mm for 10 min. none of the oils showed bactericidal activity when used alone. soy sauce combined with carvacrol ...	2016	26490647
isolation, identification, and evaluation of novel probiotic strains isolated from feces of breast-fed infants.	to isolate, identify, and evaluate the probiotic properties of lactic acid bacteria (lab) isolated from the feces of breast-fed infants.	2016	26817236
vaccines in prophylaxis of urinary tract infections caused by the bacteria from the genus proteus.	urinary tract infections (utis) pose a threat especially to women, the individuals with weakened immunity or with abnormalities in the urinary tract as well as to hospitalized and catheterized patients. the bacteria from the genus proteus, especially p. mirabilis, are important uti pathogenic factors. they frequently cause chronic, recurrent or severely complicated infections, resulting in the urinary stones production due to urease and other virulence factors. the ability to survive inside the ...	2016	27708208
in vitro assessment of the antimicrobial potentials of lactobacillus helveticus strains isolated from traditional cheese in sinkiang china against food-borne pathogens.	lactobacillus helveticus, an obligatory hetero-fermentative lab, is generally recognized as safe (gras) and is gaining popularity for application in dairy products. lactic acid bacteria (lab) play a remarkable role in inhibiting the growth of pathogenic bacteria in food products, without disturbing the sensory attributes of the food. in this study, the screening of the antimicrobial potential of lactobacillus helveticus klds 1.8701 against four food-borne pathogens including listeria monocytogen ...	2016	26648272
chemopreventive effect and lack of genotoxicity and mutagenicity of the exopolysaccharide botryosphaeran on human lymphocytes.	carbohydrate biopolymers of fungal-origin are an important natural resource in the search for new bioagents with therapeutic and nutraceutical potential. in this study the mutagenic, genotoxic, antigenotoxic and antioxidant properties of the fungal exopolysaccharide botryosphaeran, a (1→3)(1→6)-β-d-glucan, from botryosphaeria rhodina mamb-05, was evaluated. the mutagenicity was assessed at five concentrations in salmonella typhimurium by the ames test. normal and tumor (jurkat cells) human t lym ...	2016	27387458
use of potential probiotic lactic acid bacteria (lab) biofilms for the control of listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7 biofilms formation.	use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries. the aims of this study were (i) to evaluate the probiotic properties of bacteriocinogenic (lactococcus lactis vb69, l. lactis vb94, lactobacillus sakei mbsa1, and lactobacillus curvatus mbsa3) and non-bacteriocinogenic (l. lactis 368, lactobacillus helveticus 354, lactobacillus casei 40, and weissela viridescens 113) lactic acid bacteria (lab) isolated from brazilian's f ...	2016	27375584
structural and functional characterization of the lps transporter lptde from gram-negative pathogens.	incorporation of lipopolysaccharide (lps) into the outer membrane of gram-negative bacteria is essential for viability, and is accomplished by a two-protein complex called lptde. we solved crystal structures of the core lptde complexes from yersinia pestis, klebsiella pneumoniae, pseudomonas aeruginosa, and a full-length structure of the k. pneumoniae lptde complex. our structures adopt the same plug and 26-strand β-barrel architecture found recently for the shigella flexneri and salmonella typh ...	2016	27161977
genomic analysis of salmonella enterica serovar typhimurium characterizes strain diversity for recent u.s. salmonellosis cases and identifies mutations linked to loss of fitness under nitrosative and oxidative stress.	salmonella enterica serovar typhimurium is one of the most common s. enterica serovars associated with u.s. foodborne outbreaks. s. typhimurium bacteria isolated from humans exhibit wide-ranging virulence phenotypes in inbred mice, leading to speculation that some strains are more virulent in nature. however, it is unclear whether increased virulence in humans is related to organism characteristics or initial treatment failure due to antibiotic resistance. strain diversity and genetic factors co ...	2016	26956590
prophylactic administration of vector-encoded porcine granulocyte-colony stimulating factor reduces salmonella shedding, tonsil colonization, and microbiota alterations of the gastrointestinal tract in salmonella-challenged swine.	salmonella colonization of food animals is a concern for animal health and public health as a food safety risk. various obstacles impede the effort to reduce asymptomatic salmonella carriage in food animals, including the existence of numerous serovars and the ubiquitous nature of salmonella. to develop an intervention strategy that is non-specific yet effective against diverse salmonella serovars, we explored the prophylactic use of a cytokine to decrease salmonella in swine by boosting the hos ...	2016	27610361
invasive bacterial infections in gambians with sickle cell anemia in an era of widespread pneumococcal and hemophilus influenzae type b vaccination.	there is relatively little data on the etiology of bacterial infections in patients with sickle cell anemia (sca) in west africa, and no data from countries that have implemented conjugate vaccines against both streptococcus pneumoniae and haemophilus influenzae type b (hib).we conducted a retrospective analysis of sca patients admitted to the medical research council unit, the gambia, during a 5-year period when there was high coverage of hib and pneumococcal conjugate vaccination. we evaluated ...	2016	27930540
immunopotentiation of different adjuvants on humoral and cellular immune responses induced by ha1-2 subunit vaccines of h7n9 influenza in mice.	in spring 2013, human infections with a novel avian influenza a (h7n9) virus were reported in china. the number of cases has increased with over 200 mortalities reported to date. however, there is currently no vaccine available for the h7 subtype of influenza a virus. virus-specific cellular immune responses play a critical role in virus clearance during influenza infection. in this study, we undertook a side-by-side evaluation of two different adjuvants, salmonella typhimurium flagellin (flic) ...	2016	26930068
multi-subtype influenza virus-like particles incorporated with flagellin and granulocyte-macrophage colony-stimulating factor for vaccine design.	virus-like particle (vlp) technology is an attractive platform for seasonal and pandemic influenza vaccine development. we previously showed that influenza vlps can be modified using m2 fusion with molecular adjuvants such as salmonella typhimurium flagellin (flic) to enhance vlp immunogenicity. for this study, three types of chimeric vlps were incorporated with flic, granulocyte-macrophage colony-stimulating factor (gm-csf), or both gm-csf and flic (gm-csf/flic) to enhance anti-influenza immuno ...	2016	27491439
a novel peptidoglycan d,l-endopeptidase induced by salmonella inside eukaryotic cells contributes to virulence.	bacteria remodel peptidoglycan structure in response to environmental changes. many enzymes are involved in peptidoglycan metabolism; however, little is known about their responsiveness in a defined environment or the modes they assist bacteria to adapt to new niches. here, we focused in peptidoglycan enzymes that intracellular bacterial pathogens use inside eukaryotic cells. we identified a peptidoglycan enzyme induced by salmonella enterica serovar typhimurium in fibroblasts and epithelial cel ...	2016	26462856
structural and biochemical studies reveal a putative ftsz recognition site on the z-ring stabilizer zapd.	ftsz, a tubulin homologue, is an essential protein of the z-ring assembly in bacterial cell division. it consists of two domains, the n-terminal and c-terminal core domains, and has a conserved c-terminal tail region. lateral interactions between ftsz protofilaments and several z-ring associated proteins (zaps) are necessary for modulating z-ring formation. zapd, one of the positive regulators of z-ring assembly, directly binds to the c-terminal tail of ftsz and promotes stable z-ring formation ...	2016	27871169
the antioxidant and antigenotoxic potential of methanol extract of cladonia foliacea (huds.) willd.	in this article, the genotoxic and antigenotoxic effects of methanol extract of of cladonia foliacea (huds.) willd. (cme) were studied using wp2, ames (ta1535 and ta1537), and sister chromatid exchange (sce) test systems. the results of our studies showed that 5 µm concentration of aflatoxin b1(afb1) changed the frequencies of sce and malondialdehyde (mda) levels, superoxide dismutase (sod), glutathione (gsh), and glutathione peroxidase (gpx) activities. when 5 and 10 µg/ml concentrations of cme ...	2016	24193055
regulatory rnas and target mrna decay in prokaryotes.	recent advances in prokaryote genetics have highlighted the important and complex roles of small regulatory rnas (srnas). although blocking mrna translation is often the main function of srnas, these molecules can also induce the degradation of target mrnas using a mechanism that drastically differs from eukaryotic rna interference (rnai). whereas rnai relies on rnase iii-like machinery that is specific to double-strand rnas, srna-mediated mrna degradation in escherichia coli and samonella typhi ...	2016	23500183
salmonella employs multiple mechanisms to subvert the tlr-inducible zinc-mediated antimicrobial response of human macrophages.	we aimed to characterize antimicrobial zinc trafficking within macrophages and to determine whether the professional intramacrophage pathogen salmonella enterica serovar typhimurium (s typhimurium) subverts this pathway. using both escherichia coli and s typhimurium, we show that tlr signaling promotes the accumulation of vesicular zinc within primary human macrophages. vesicular zinc is delivered to e. coli to promote microbial clearance, whereas s. typhimurium evades this response via salmonel ...	2016	26839376
characterization of slya in shigella flexneri identifies a novel role in virulence.	the slya transcriptional regulator has important roles in the virulence and pathogenesis of several members of the enterobacteriaceae family, including salmonella enterica serovar typhimurium and escherichia coli. despite the identification of the slya gene in shigella flexneri nearly 2 decades ago, as well as the significant conservation of slya among enteric bacteria, the role of slya in shigella remains unknown. the genes regulated by slya in closely related organisms often are absent from or ...	2016	26831468
in vitro effects of thymol-β-d-glucopyranoside on salmonella enterica serovar typhimurium and escherichia coli k88.	although thymol is bactericidal against many pathogens in vitro, its in vivo effectiveness against pathogens in the lower gastrointestinal tract is limited because of its rapid absorption in the proximal gut. thymol-β-<small>d</small>-glucopyranoside (β-thymol), a conjugated form of thymol, can deliver thymol to the lower gastrointestinal tract and has shown antibacterial effects. in the present study, we examined the in vitro effects of β-thymol on salmonella enterica serovar typhimurium (st) a ...	2016	26818992
pharmaco-phylogenetic investigation of methyl gallate isolated from acacia nilotica (l.) delile and its cytotoxic effect on nih3t3 mouse fibroblast.	present exploration deals with the therapeutic perspective of methyl gallate isolated from the leaf extract of acacia nilotica (l.) delile in contrast to food-borne bacterial pathogen's viz., escherichia coli, klebsiella pneumoniae, salmonella typhimurium, pseudomonas aeruginosa and staphylococcus aureus with their evolutionary succession. the extract was subjected to phytochemical analysis and isolated compound was identified as methyl gallate using uv-vis, ir and nmr spectra. it was found most ...	2016	26813302
loop-to-helix transition in the structure of multidrug regulator acrr at the entrance of the drug-binding cavity.	multidrug transcription regulator acrr from salmonella enterica subsp. enterica serovar typhimurium str. lt2 belongs to the tetracycline repressor family, one of the largest groups of bacterial transcription factors. the crystal structure of dimeric acrr was determined and refined to 1.56å resolution. the tertiary and quaternary structures of acrr are similar to those of its homologs. the multidrug binding site was identified based on structural alignment with homologous proteins and has a di(hy ...	2016	26796657
the magnesium transporter a is activated by cardiolipin and is highly sensitive to free magnesium in vitro.	the magnesium transporter a (mgta) is a specialized p-type atpase, believed to import mg(2+) into the cytoplasm. in salmonella typhimurium and escherichia coli, the virulence determining two-component system phoq/phop regulates the transcription of mgta gene by sensing mg(2+) concentrations in the periplasm. however, the factors that affect mgta function are not known. this study demonstrates, for the first time, that mgta is highly dependent on anionic phospholipids and in particular, cardiolip ...	2016	26780187
photoexcited quantum dots for killing multidrug-resistant bacteria.	multidrug-resistant bacterial infections are an ever-growing threat because of the shrinking arsenal of efficacious antibiotics. metal nanoparticles can induce cell death, yet the toxicity effect is typically nonspecific. here, we show that photoexcited quantum dots (qds) can kill a wide range of multidrug-resistant bacterial clinical isolates, including methicillin-resistant staphylococcus aureus, carbapenem-resistant escherichia coli, and extended-spectrum β-lactamase-producing klebsiella pneu ...	2016	26779882
generation of salmonella ghost cells expressing fimbrial antigens of enterotoxigenic escherichia coli and evaluation of their antigenicity in a murine model.	salmonella typhimurium ghost cells expressing k88ab, k88ac, k99, and fasa fimbriae of enterotoxigenic escherichia coli (etec) in their envelopes were constructed. the genes encoding the fimbriae were individually cloned into an expression plasmid, pmmp81, carrying the asd gene, which was subsequently electroporated into the δasd s. typhimurium mutant. plasmid pjhlp99, carrying the phix174 lysis gene e, was also subsequently electroporated into the salmonella mutant. the presence of the individua ...	2016	26733731
effect of high intensity ultrasound on the fermentation profile of lactobacillus sakei in a meat model system.	the objective of this study was to investigate the efficacy of high intensity ultrasound on the fermentation profile of lactobacillus sakei in a meat model system. ultrasound power level (0-68.5 w) and sonication time (0-9 min) at 20 °c were assessed against the growth of l. sakei using a microplate reader over a period of 24h. the l. sakei growth data showed a good fit with the gompertz model (r(2)>0.90; se<0.042). second order polynomial models demonstrated the effect of ultrasonic power and s ...	2016	26964981
bovine natural antibodies in antibody-dependent bactericidal activity against escherichia coli and salmonella typhimurium and risk of mastitis.	natural antibodies (nabs) are mostly igm antibodies produced without antigenic stimulation and serve as a first line of defence of the immune system. as both natural and specific antibodies are present in animals, nabs are studied by determining the igm response to naïve antigens like keyhole limpet hemocyanin (klh). in this study, we selected cows based on high and low anti-klh igm titers, reflecting high and low nab titers, and determined if the anti-klh igm titers were indicative for the reco ...	2016	26964714
inactivation or inhibition of acrab-tolc increases resistance of carbapenemase-producing enterobacteriaceae to carbapenems.	the objective of this study was to study the contribution of the multidrug resistance acrab-tolc efflux system to carbapenem resistance in carbapenemase-producing enterobacteriaceae and the impact of the efflux inhibitor pabn on this resistance.	2016	26945714
microbiological food safety status of commercially produced tomatoes from production to marketing.	tomatoes have been implicated in various microbial disease outbreaks and are considered a potential vehicle for foodborne pathogens. traceback studies mostly implicate contamination during production and/or processing. the microbiological quality of commercially produced tomatoes was thus investigated from the farm to market, focusing on the impact of contaminated irrigation and washing water, facility sanitation, and personal hygiene. a total of 905 samples were collected from three largescale ...	2016	26939649
photo-active float for field water disinfection.	the present study investigates the antibacterial activity of a photoactive float fabricated with visible light active n-f-tio2 for the disinfection of field water widely contaminated with gram positive and gram negative bacteria like, salmonella typhimurium (gram negative), escherichia coli (gram negative), staphylococcus aureus (gram positive), bacillus species (gram positive), and pseudomonas species (gram negative). the antibacterial activity can be attributed to the unique properties of the ...	2016	26924232
extraction method plays critical role in antibacterial activity of propolis-loaded hydrogels.	extracted propolis has been used for a long time as a remedy. however, if the release rate of propolis is not controlled, the efficacy is reduced. to overcome this issue, extracted propolis was added to a cryogel system. propolis collected from southern brazil was extracted using different methods and loaded at different concentrations into polyvinyl alcohol (pva) and polyacrylic acid hydrogels as carrier systems. the material properties were investigated with a focus on the propolis release pro ...	2016	26886307
centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water.	waterborne pathogens pose significant threat to the global population and early detection plays an important role both in making drinking water safe, as well as in diagnostics and treatment of water-borne diseases. we present an innovative centrifugal sedimentation immunoassay platform for detection of bacterial pathogens in water. our approach is based on binding of pathogens to antibody-functionalized capture particles followed by sedimentation of the particles through a density-media in a mic ...	2016	26858815
biofabrication of zinc oxide nanoparticles using fruit extract of rosa canina and their toxic potential against bacteria: a mechanistic approach.	the use of plant extract in the biosynthesis of nanoparticles (nps) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. in this study, the biosynthesis of zinc oxide (zno) nps by both "conventional heating" (ch) and "microwave irradiation" (mi) methods has been reported. stable and spherical znonps were produced using zinc nitrate and flesh extract of rosa canina fruit (rosehip) which was used as a precurso ...	2016	26652376
multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis.	this study reports multiplex sorting of salmonella typhimurium and escherichia coli 0157, from broth cultures and from pathogen-spiked skinned chicken breast enrichment broths by employing microfluidic free-flow magnetophoresis. magnetic beads of different sizes and magnetite content, namely dynabeads anti-salmonella and hyglos-streptavidin beads together with the corresponding pathogen-specific biotinylated recombinant phages, were utilised as affinity solid phases for the capture and concentra ...	2016	27046212
attenuated salmonella typhimurium delivery of a novel dna vaccine induces immune responses and provides protection against duck enteritis virus.	dna vaccines are widely used to prevent and treat infectious diseases, cancer and autoimmune diseases; however, their relatively low immunogenicity is an obstacle to their use. in this study, we constructed a novel and universal dna vaccine vector (pss898) that can be used to build dna vaccines against duck enteritis virus (dev) and other viruses that require dna vaccines to provide protection. this vaccine vector has many advantages, including innate immunogenicity, efficient nuclear traffickin ...	2016	27016773
plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis.	we describe the design, fabrication, and successful demonstration of a sample preparation module comprising bacteria cell capture and thermal lysis on-chip with potential applications in food sample pathogen analysis. plasma nanotexturing of the polymeric substrate allows increase of the surface area of the chip and the antibody binding capacity. three different anti-salmonella antibodies were directly and covalently linked to plasma treated chips without any additional linker chemistry or other ...	2016	26556673
multi-parameter flow cytometry as a process analytical technology (pat) approach for the assessment of bacterial ghost production.	flow cytometry (fcm) is a tool for the analysis of single-cell properties in a cell suspension. in this contribution, we present an improved fcm method for the assessment of e-lysis in enterobacteriaceae. the result of the e-lysis process is empty bacterial envelopes-called bacterial ghosts (bgs)-that constitute potential products in the pharmaceutical field. bgs have reduced light scattering properties when compared with intact cells. in combination with viability information obtained from stai ...	2016	26521248
amino acid substitutions in gyra affect quinolone susceptibility in salmonella typhimurium.	the prevalence of quinolone-resistant salmonella has become a public health concern. amino acid substitutions have generally been found within the quinolone resistance-determining region in subunit a of dna gyrase (gyra) of salmonella typhimurium. however, direct evidence of the contribution of these substitutions to quinolone resistance remains to be shown. to investigate the significance of amino acid substitutions in s. typhimurium gyra to quinolone resistance, we expressed recombinant wild-t ...	2016	26514939