Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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bloodstream infection caused by stenotrophomonas maltophilia in children. | stenotrophomonas maltophilia, a multidrug resistant gram-negative pathogen, has become a more frequent cause of bloodstream infections (bsi). little is known about development of s. maltophilia bacteremia in children. the objective of this study was to define risk factors and outcomes associated with s. maltophilia bsi in children. | 2007 | 17529868 |
characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in qinghai-tibet plateau and in other zones of china. | qinghai-tibet plateau is the highest place in the world and the environment in that plateau is hard for animals and plants, with low temperature, low concentration of oxygen and high solar radiation. in this study, 61 root nodule isolates from vicia, oxytropis, medicago, melilotus and onobrychis species grown in qinghai-tibet plateau and in loess plateau were comparatively characterized. based upon the results of numerical taxonomy, ardra, aflp, dna-dna hybridization and 16s rdna sequencing, the ... | 2007 | 17541555 |
global emergence of trimethoprim/sulfamethoxazole resistance in stenotrophomonas maltophilia mediated by acquisition of sul genes. | trimethoprim/sulfamethoxazole (tmp/smx) resistance remains a serious threat in the treatment of stenotrophomonas maltophilia infections. we analyzed an international collection of 55 s. maltophilia tmp/smx-sensitive (s) (n=30) and -resistant (r) (n=25) strains for integrons; sul1, sul2 and dhfr genes; and insertion element common region (iscr) elements. sul1, as part of a class 1 integron, was detected in 17 of 25 tmp/smx-r. nine tmp/smx-r strains carried sul2; 7 were on large plasmids. five tmp ... | 2007 | 17553270 |
emerging and unusual gram-negative infections in cystic fibrosis. | people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ... | 2007 | 17562501 |
microbiology of ventilator-associated pneumonia compared with that of hospital-acquired pneumonia. | nosocomial pneumonia is the leading cause of mortality attributed to nosocomial infection. appropriate empirical therapy has been associated with improved survival, but data are limited regarding the etiologic agents of hospital-acquired pneumonia in nonventilated patients (hap). this evaluation assessed whether the currently recommended empirical therapy is appropriate for both ventilator-associated pneumonia (vap) and hap by evaluating the infecting flora. | 2007 | 17564985 |
fatal pulmonary hemorrhage in a patient with myelodysplastic syndrome and fulminant pneumonia caused by stenotrophomonas maltophilia. | 2007 | 17565466 | |
effect of environmental factors on biofilm formation by clinical stenotrophomonas maltophilia isolates. | the influence of environmental factors (temperature, aerobiosis-anaerobiosis, static-dynamic conditions, ph) was determined on biofilm formation by 51 s. maltophilia clinical isolates. the strains produced more biofilm at 32 degrees c than at 37 or 18 degrees c. aerobic and 6% co2 atmosphere yielded comparable biofilm amounts, higher than under anaerobic conditions. biofilm production was not affected by static vs. agitated culture conditions. biofilm production at ph 7.5 and 8.5 was comparable ... | 2007 | 17571802 |
successful engraftment of the second reduced-intensity conditioning cord blood transplantation (cbt) for a patient who developed graft rejection and infectious complications after the first cbt for aml. | 2007 | 17572713 | |
a cyclic amp receptor protein-regulated cell-cell communication system mediates expression of a feca homologue in stenotrophomonas maltophilia. | stenotrophomonas maltophilia wr-c possesses an rpf/diffusible signal factor (dsf) cell-cell communication system. it produces cis-delta2-11-methyl-dodecenoic acid, a dsf, and seven structural derivatives, which require rpff and rpfb for synthesis. acquisition of iron from the environment is important for bacterial growth as well as the expression of virulence genes. we identified a gene homologous to feca, which encodes a ferric citrate receptor that transports exogenous siderophore ferric citra ... | 2007 | 17574998 |
isotopic analysis of n and o in nitrite and nitrate by sequential selective bacterial reduction to n2o. | nitrite is an important intermediate species in the biogeochemical cycling of nitrogen, but its role in natural aquatic systems is poorly understood. isotopic data can be used to study the sources and transformations of no2- in the environment, but methods for independent isotopic analyses of no2- in the presence of other n species are still new and evolving. this study demonstrates that isotopic analyses of n and o in no2- can be done by treating whole freshwater or saltwater samples with the d ... | 2007 | 17580983 |
[clinical distribution and antibiotic resistance of non-fermentative gram-negative bacilli infection after liver transplantation]. | to investigate the epidemiology of non-fermentative gram-negative bacilli infections after liver transplantation, and to analyze the antibiotic susceptibility to major pathogens. | 2007 | 17593853 |
risk factors for infections with multidrug-resistant stenotrophomonas maltophilia in patients with cancer. | stenotrophomonas maltophilia is responsible for an increasing number of infections, especially in hospitalized patients. therapy options are limited and trimethoprim/sulfamethoxazole (tmp/smx) is often the main treatment option for this infection. in the current study, the risk factors were determined for the emergence of multidrug-resistant (mdr) s. maltophilia. | 2007 | 17487860 |
thirteen-lined ground squirrels (spermophilus tridecemlineatus) harbor multiantibiotic-resistant bacteria. | whether wild-caught animals used for biomedical research carry antibiotic-resistant bacteria is not well studied. thirteen- lined ground squirrels (spermophilus tridecemlineatus) are small mammals used to study hibernation. these animals are captured from the wild or are born in laboratory animal facilities to wild-caught mothers. because microorganisms harbored by 13-lined ground squirrels may be pathogenic to their caretakers and other laboratory animals, learning more about antibiotic resista ... | 2007 | 17487947 |
comparative review of the carbapenems. | the carbapenems are beta-lactam antimicrobial agents with an exceptionally broad spectrum of activity. older carbapenems, such as imipenem, were often susceptible to degradation by the enzyme dehydropeptidase-1 (dhp-1) located in renal tubules and required co-administration with a dhp-1 inhibitor such as cilastatin. later additions to the class such as meropenem, ertapenem and doripenem demonstrated increased stability to dhp-1 and are administered without a dhp-1 inhibitor. like all beta-lactam ... | 2007 | 17488146 |
[primary intracerebral hemorrhage due to probable cerebral amyloid angiopathy complicated by brain abscess: case report]. | cerebral amyloid angiopathy (caa), in which deposition of amyloid within the arterial media and adventitia is the remarkable feature, causes spontaneous lober cerebral hemorrhage in elderly person, and some reports show the quite high occurrence rate of this entity among intracerebral hemorrhage in patents above 70 years old. brain abscess resulting from intracerebral hemorrhage is rare. to our knowledge, no report of such hemorrhage which is caused by caa has been published so far. we report a ... | 2007 | 17491345 |
[mesenteric vein thrombosis and protein c and s deficiency in a patient with chronic hepatitis c on treatment with interferon and ribavirin]. | we describe the case of a 67 year old male with chronic hepatitis c on treatment with pegylated interferon and ribavirin who, after two and a half months of combined treatment, presented with a picture of acute mesenteric vein thrombosis that required urgent surgery. it re-occurred several days later and was his cause of death. in the thrombophilia study carried out immediately after surgery a decrease in protein c and s was considered as a cause of hypercoagulability. protein c and s deficiency ... | 2007 | 17493436 |
evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing. | ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ... | 2007 | 17507509 |
in vitro activity of tigecycline against clinical isolates of acinetobacter baumannii and stenotrophomonas maltophilia. | 2007 | 17213264 | |
enhanced hydroxylation of imidacloprid by stenotrophomonas maltophilia upon addition of sucrose. | sucrose's ability to promote the hydroxylation of imidacloprid (imi) by bacterium stenotrophomonas maltophilia strain cgmcc 1.1788 was examined. both growing culture and resting cells could transform imi into 5-hydroxy imi. adding 2% sucrose to the growing culture transformation broth and 5% sucrose to the resting cell transformation broth resulted in biotransformation yields, respectively, 2.5 and 9 times greater than without sucrose. in the growing culture transformation, sucrose increased bio ... | 2007 | 17216456 |
bacteria associated with orchid roots and microbial production of auxin. | associative bacteria of terrestrial (paphiopedilum appletonianum) and epiphytic (pholidota articulata) tropical orchids were investigated. microbial community of epiphytic plant differed from that of the terrestrial one. streptomyces, bacillus, pseudomonas, burkholderia, erwinia and nocardia strains populated paphiopedilum roots, whereas pseudomonas, flavobacterium, stenotrophomonas, pantoea, chryseobacterium, bacillus, agrobacterium, erwinia, burkholderia and paracoccus strains colonized pholid ... | 2007 | 17140781 |
serological cross-reactivity of environmental isolates of enterobacter, escherichia, stenotrophomonas, and aerococcus with shigella spp.-specific antisera. | using protocols designed for the isolation of shigella from environmental freshwater samples from different regions of bangladesh, 11 bacterial strains giving rise to shigella-like colonies on selective agar plates and showing serological cross-reaction with shigella-specific antisera were isolated. phylogenetic analyses revealed that three of the isolates were most closely related to escherichia coli, four to enterobacter sp., two to stenotrophomonas, and two isolates belonged to the gram-posit ... | 2007 | 17171463 |
influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant. | microbiological contamination of manipulated blood products, including hematopoietic progenitors obtained from peripheral blood, is an infrequent but persistent problem in transplant units. the relevance of such contamination in causing patient infection has been reported as insignificant, but the effect on the post-transplant course has not been well documented. we studied the incidence of bacterial contamination in autologous peripheral blood progenitor cell transplants in two of the bench pro ... | 2007 | 17173054 |
volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen rhizoctonia solani. | bacterial antagonists are bacteria that negatively affect the growth of other organisms. many antagonists inhibit the growth of fungi by various mechanisms, e.g., secretion of lytic enzymes, siderophores and antibiotics. such inhibition of fungal growth may indirectly support plant growth. here, we demonstrate that small organic volatile compounds (vocs) emitted from bacterial antagonists negatively influence the mycelial growth of the soil-borne phytopathogenic fungus rhizoctonia solani kühn. s ... | 2007 | 17180381 |
stenotrophomonas maltophilia pneumonia in cancer patients without traditional risk factors for infection, 1997-2004. | in order to elucidate the spectrum of stenotrophomonas maltophilia pneumonia in cancer patients without traditional risk factors, 44 cancer patients (cases) with s. maltophilia pneumonia in whom s. maltophilia pneumonia risk factors were not present were compared with two s. maltophilia pneumonia risk groups (controls) including 43 neutropenic non-intensive care unit (icu) and 21 non-neutropenic icu patients. the case and control patients had similar demographic and underlying clinical character ... | 2007 | 17200840 |
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
isolation of bacteria able to grow on both polyethylene glycol (peg) and polypropylene glycol (ppg) and their peg/ppg dehydrogenases. | two bacterial consortia growing on a random copolymer of ethylene glycol and propylene glycol units were obtained by enrichment cultures from various microbial samples. six major strains included in both consortia were purified and identified as sphingomonads, pseudomonas sp. and stenotrophomonas maltophilia. three of them (sphingobium sp. strain ek-1, sphingopyxis macrogoltabida strain ey-1, and pseudomonas sp. strain pe-2) utilized both peg and polypropylene glycol (ppg) as a sole carbon sourc ... | 2007 | 17043822 |
preparation of 3-ketovalidoxylamine a c-n lyase substrate: n-p-nitrophenyl-3-ketovalidamine by stenotrophomonas maltrophilia cctcc m 204024. | 3-ketovalidoxylamine a c-n lyase is one of three key enzymes in the production of valienamine, which is a potent glucosidase inhibitor from validamycin a. n-p-nitrophenyl-3-ketovalidamine, used as the substrate of 3-ketovalidoxylamine a c-n lyase, was prepared from n-p-nitrophenylvalidamine with free cells of stenotrophomonas maltrophilia cctcc m 204024. the yield and selectivity of n-p-nitrophenyl-3-ketovalidamine from cells were improved by treatment with 10 mm ethylenediaminetetraacetic acid. ... | 2007 | 17058074 |
aph(3')-iic, an aminoglycoside resistance determinant from stenotrophomonas maltophilia. | we report the characterization of an intrinsic, chromosomally carried aph(3')-iic gene from stenotrophomonas maltophilia clinical isolate k279a, encoding an aminoglycoside phosphotransferase enzyme that significantly increases mics of kanamycin, neomycin, butirosin, and paromomycin when expressed in escherichia coli. disruption of aph(3')-iic in k279a results in decreased mics of these drugs. | 2007 | 17088477 |
first reported infections caused by three newly described genera in the family xanthomonadaceae. | members of the family of xanthomonadaceae are typically characterized as environmental organisms. with the exception of stenotrophomonas maltophilia, these organisms are infrequently implicated as human pathogens. we describe three cases of central venous catheter-associated bloodstream infections caused by dokdonella koreensis, aquimonas voraii, and a luteibacter sp., all newly named genera within the family xanthomonadaceae. the three patients all had histories of underlying hematological diso ... | 2007 | 17122001 |
characterization of small-colony-variant stenotrophomonas maltophilia isolated from the sputum specimens of five patients with cystic fibrosis. | cystic fibrosis (cf) patients are predisposed to chronic respiratory infection by nonfermentative gram-negative bacilli, including stenotrophomonas maltophilia. s. maltophilia is highly resistant to most antibiotics, with the exception of sulfamethoxazole-trimethoprim (sxt). sxt-resistant s. maltophilia has been reported, but the mechanism of resistance is not well defined. repeated findings of suspected small-colony-variant (scv) s. maltophilia isolates from the sputa of five cf patients were c ... | 2007 | 17135443 |
immunostimulatory properties of the emerging pathogen stenotrophomonas maltophilia. | stenotrophomonas maltophilia is a multiple-antibiotic-resistant opportunistic pathogen that is being isolated with increasing frequency from patients with health-care-associated infections and especially from patients with cystic fibrosis (cf). while clinicians feel compelled to treat infections involving this organism, its potential for virulence is not well established. we evaluated the immunostimulatory properties and overall virulence of clinical isolates of s. maltophilia using the well-cha ... | 2007 | 17220304 |
infectious complications of percutaneous central venous catheterization in pediatric patients: a spanish multicenter study. | analysis of infectious complications and risk factors in percutaneous central venous catheters. | 2007 | 17235512 |
monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses. | the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ... | 2007 | 17259356 |
stability of a promiscuous plasmid in different hosts: no guarantee for a long-term relationship. | broad-host-range (bhr) incp-1 plasmids have the ability to transfer between and replicate in nearly all species of the alpha-, beta- and gammaproteobacteria, but surprisingly few data are available on the stability of these plasmids in strains within their host range. moreover, even though molecular interactions between the bacterial host and its plasmid(s) exist, no systematic study to date has compared the stability of the same plasmid among different hosts. the goal of this study was to exami ... | 2007 | 17259616 |
munchausen syndrome by proxy and recurrent polymicrobial bacteremia. | 2007 | 17259892 | |
an acquired efflux system is responsible for copper resistance in xanthomonas strain ig-8 isolated from china. | the genus xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. in other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. this report describes the isolation of a novel gene cluster xmersa from xanthomonas strain ig-8 that mediates copper chloride resistance. subsequent analysis of these genes showed that they were responsible for the high level o ... | 2007 | 17263848 |
identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india. | lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ... | 2007 | 17264993 |
stenotrophomonas maltophilia pneumonia after bone marrow transplantation: case report with emphasis on the high-resolution ct findings. | a 6-year-old female patient who underwent bone marrow transplantation because of fanconi anaemia presented with fever, dyspnoea and cough 17 days after the procedure. the physical examination revealed diffuse crackles. chest radiographs demonstrated diffuse alveolar opacities in both lungs. high-resolution ct showed a diffuse and bilateral lung lesion characterized by multifocal areas of air-space consolidation associated with ground-glass attenuation and small centrilobular nodules. the culture ... | 2007 | 17267464 |
stenotrophomonas maltophilia as a part of normal oral bacterial flora in captive snakes and its susceptibility to antibiotics. | only little is known about normal oral bacterial flora in captive snakes containing stenotrophomonas maltophilia. this microbe has been reported as a causative agent of numerous infections in reptiles. therefore, the goal of the study was to detect its presence in the mouths of a significant number of healthy captive snakes and determining its susceptibility to antibiotics at 30 and 37 degrees c. the isolates were obtained in 1999-2005 from mouth swabs of 115 snakes of 12 genera and 22 species-m ... | 2007 | 17276020 |
enrichment and isolation of endosulfan degrading and detoxifying bacteria. | in the present study, degradation of endosulfan by a mixed culture isolated from a pesticide-contaminated soil was studied in batch experiments. after two weeks of incubation, the mixed culture was able to degrade 73% and 81% of alpha and beta endosulfan respectively. endodiol was identified by gc/ms as degradation intermediate. the toxicity studies of endosulfan before and after degradation were carried out using micronucleus assay on human polymorphonuclear cells. the findings suggested that t ... | 2007 | 17289112 |
application of molecular methods for analysing the distribution and diversity of acetic acid bacteria in chilean vineyards. | the presence of acetic acid bacteria populations on grape surfaces from several chilean valleys is reported. the bacteria were analysed at both the species and the strain level by molecular methods such as rflp-pcr 16s rrna gene, rflp-pcr its 16s-23s rrna gene regions and arbitrary primed (ap) pcr. our results show that there are limited numbers of species of acetic acid bacteria in the grapes and that there is a need for an enrichment medium before plating to recover the individual colonies. in ... | 2007 | 17289199 |
cystic fibrosis in adults. | the aim of this cross-sectional retrospective study was to describe clinical and functional characteristics and the microbiological profile of an adult population with cystic fibrosis. the study was performed at the pulmonary diseases service of the hospital of the medical school of the state university of campinas. the charts of 54 adults (27 males, 27 females) with cystic fibrosis were reviewed. demographic, clinical, and microbiological data were collected. clinical and functional characteris ... | 2007 | 17294339 |
the impact of multidrug resistance on the outcomes of critically ill patients with gram-negative bacterial pneumonia. | multidrug resistance (mdr) in gram-negative bacteria is on the rise, but its effect on patient outcomes is not well established. the outcomes of 129 adult surgical intensive care unit (sicu) patients treated for gram-negative pneumonia were evaluated in relation to demographics, bacterial etiology, and infections due to mdr bacteria (defined as resistant to all agents except for aminoglycosides and polymyxins). the mean (sd) age and acute physiology and chronic health evaluation (apache) ii scor ... | 2007 | 17300905 |
benzoic acid-degrading bacteria from the intestinal tract of macrotermes michaelseni sjöstedt. | the intestinal tracts of termites host a wide variety of microbial symbionts, which have been implicated in degradative processes. in this study, a fungus-cultivating termite, macrotermes michaelseni was found to harbor 2.2 x 10(6) bacterial cells per ml of gut homogenates capable of degrading benzoic acid. two benzoic acid degrading bacteria were isolated from the highest dilution of gut homogenates in oxic media with benzoic acid as the sole carbon source. isolate cbc was related to stenotroph ... | 2007 | 17304624 |
isolation and identification of a novel valienamine-producing bacterium. | to isolate, identify and assess valienamine production by a soil bacterial isolate from a wheat field in hangzhou, china. | 2007 | 17309634 |
emerging cystic fibrosis pathogens: incidence and antimicrobial resistance. | we examined the frequency of isolation and the antimicrobial resistance of burkholderia cepacia complex, stenotrophomonas maltophilia and achromobacter xylosoxidans in cystic fibrosis patients from 2000 to 2004. strains susceptibility to tobramycin, piperacillin/tazobactam, imipenem, gentamicin, ciprofloxacin and ceftazidime was determined by disc diffusion assay. b. cepacia complex showed a very high resistance also to ciprofloxacin reaching 100% in 2004. s. maltophilia and a. xvylosoxidans sho ... | 2007 | 17319602 |
detection of dna from a range of bacterial species in the knee joints of dogs with inflammatory knee arthritis and associated degenerative anterior cruciate ligament rupture. | mixtures of bacterial nucleic acids can often be detected in synovial joints affected with arthritis. we investigated the potential role of such mixtures of bacterial nucleic acids in the pathogenesis of arthritis in a naturally occurring canine model. dogs with a common inflammatory knee arthritis in which associated pathological degenerative anterior cruciate ligament (acl) rupture often develops were studied. synovial biopsies were obtained from 43 dogs with the naturally occurring acl ruptur ... | 2007 | 17320342 |
screening of the antibacterial effects of a variety of essential oils on microorganisms responsible for respiratory infections. | the aim of this study was to examine the cytotoxicity and the antibacterial effects of a variety of essential oils on major respiratory tract pathogens. the cytotoxicity of 13 essential oils was evaluated on vero cells. the antibacterial activity was evaluated by the kirby bauer paper method, minimum inhibitory and bactericidal concentration against streptococcus pyogenes, agalactiae, pneumoniae and klebsiella pneumoniae, haemophilus influenzae, staphylococcus aureus and stenotrophomonas maltoph ... | 2007 | 17326042 |
increased incidence of class 1 integrons in trimethoprim/sulfamethoxazole-resistant clinical isolates of stenotrophomonas maltophilia. | 2007 | 17329265 | |
antimicrobial therapy for stenotrophomonas maltophilia infections. | stenotrophomonas maltophilia has emerged as an important nosocomial pathogen capable of causing respiratory, bloodstream, and urinary infections. the treatment of nosocomial infections by s. maltophilia is difficult, as this pathogen shows high levels of intrinsic or acquired resistance to different antimicrobial agents, drastically reducing the antibiotic options available for treatment. intrinsic resistance may be due to reduced outer membrane permeability or to the multidrug efflux pumps. how ... | 2007 | 17334747 |
empirical antimicrobial treatment for chemotherapy-induced febrile neutropenia. | febrile neutropenia in immunocompromised hosts is associated with a high mortality. empirical treatment in such cases is instituted to cover the common pathogens. generally, combination antibiotic treatment is used early in the febrile neutropenia phase. recent studies demonstrate that monotherapy with certain beta-lactam antibiotics can be equally effective. glycopeptide antibiotics are used in the absence of an adequate response to the initial antibiotics. empirical antifungal therapy may be g ... | 2007 | 17346939 |
comparison of etest, vitek and agar dilution for susceptibility testing of colistin. | in total, 172 isolates of enterobacteriaceae, acinetobacter spp., pseudomonas aeruginosa and stenotrophomonas maltophilia were tested for susceptibility to colistin by agar dilution, etest and the vitek 2 system. isolates with a colistin mic < or =2 mg/l were considered to be susceptible. fifty-four (31%) gram-negative isolates were resistant to colistin. categorical agreement between agar dilution and etest was 87%, and between agar dilution and vitek 2 was 82%. based on the data obtained, the ... | 2007 | 17371537 |
pathogens in early-onset and late-onset intensive care unit-acquired pneumonia. | to compare the type of pathogens isolated from patients with early-onset intensive care unit (icu)-acquired pneumonia with those isolated from patients with late-onset icu-acquired pneumonia and to study risk factors for the isolation of pathogens that are potentially resistant to multiple drugs. | 2007 | 17385143 |
physiological and phylogenetic characterization of a stable benzene-degrading, chlorate-reducing microbial community. | a stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. the benzene degradation rate was 1.65 mm benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. denaturing gradient gel electrophoresis of part of the 16s rrna gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. five bacterial clones were ... | 2007 | 17386037 |
antibiotic binding to dizinc beta-lactamase l1 from stenotrophomonas maltophilia: scc-dftb/charmm and dft studies. | a dizinc beta-lactamase (l1 from stenotrophomonas maltophilia) complexed with an antibiotic compound (moxalactam) has been studied using a hybrid quantum mechanical/molecular mechanical (qm/mm) approach. the qm region is described by the self-consistent charge-density functional tight binding (scc-dftb) model while the mm by charmm. the michaelis complex, which is constructed from a recent x-ray structure of the l1 enzyme with the hydrolyzed moxalactam, is simulated by molecular dynamics. the si ... | 2007 | 17388313 |
presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser. | reported here are the microbiological and epidemiological details of a presumed outbreak of aerobic gram-negative bacilli infections affecting 19 hematological patients, which was traced to contaminated disinfectant. over a 5-month period, the following organisms were isolated from the blood cultures of 19 neutropenic patients: pseudomonas fluorescens (n = 13), achromobacter xylosoxidans (n = 12), comamonas testosteroni (n = 2) or stenotrophomonas maltophilia (n = 1). the affected patients were ... | 2007 | 17393202 |
faropenem: review of a new oral penem. | faropenem medoxomil is a new orally administered penem antibiotic. its chiral tetrahydrofuran substituent at position c2 is responsible for its improved chemical stability and reduced cns effects, compared with imipenem. faropenem demonstrates broad-spectrum in vitro antimicrobial activity against many gram-positive and -negative aerobes and anaerobes, and is resistant to hydrolysis by nearly all beta-lactamases, including extended-spectrum beta-lactamases and ampc beta-lactamases. however, faro ... | 2007 | 17402834 |
bacterial reduction of selenium in coal mine tailings pond sediment. | sediment from a storage facility for coal tailings solids was assessed for its capacity to reduce selenium (se) by native bacterial community. one se(6+)-reducing bacterium enterobacter hormaechei (tar11) and four se(4+)-reducing bacteria, klebsiella pneumoniae (tar1), pseudomonas fluorescens (tar3), stenotrophomonas maltophilia (tar6), and enterobacter amnigenus (tar8) were isolated from the sediment. enterobacter hormaechei removed 96% of the added se(6+) (0.92 mg l(-1)) from the effluents whe ... | 2007 | 17412898 |
relationship between immunosuppression and intensive care unit-acquired multidrug-resistant bacteria: a case-control study. | to determine the relationship between immunosuppression and intensive care unit (icu)-acquired multidrug-resistant (mdr) bacteria. | 2007 | 17414081 |
[study on the structure and function of a stable methane-oxidizing mixed microbial consortium]. | from an agricultural sample taken in chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. the mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. more than 99% of phenol at an initial concentration of 600mg/l could be degraded by the mixed microbial consortium after 11 h of cultivation. the productivity of 1, 2-epoxypropane could be increas ... | 2007 | 17436634 |
characterization of a novel t4-type stenotrophomonas maltophilia virulent phage smp14. | stenotrophomonas maltophilia (sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. in this study, a novel virulent sm phage, smp14, was characterized. electron microscopy showed that smp14 resembled members of myoviridae and adsorbed to poles of the host cells during infection. it lysed 37 of 87 clinical sm isolates in spot test, displayed a latent period of ca. 20 min, and had a burst size of ca. 150. its genome (estimated to ... | 2007 | 17440710 |
potential of the adhesion of bacteria isolated from drinking water to materials. | heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rdna gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (asi 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, ... | 2007 | 17440920 |
the efficacy of nonsurgical treatment of infected pancreatic necrosis. | we conducted this study to evaluate the efficacy of nonsurgical treatment for patients with infected pancreatic necrosis (ipn). | 2007 | 17446837 |
removal of cadmium ions during stationary growth phase by an extremely cadmium-resistant strain of stenotrophomonas sp. | stenotrophomonas sp. cd02 was isolated from a site that previously had been contaminated with high concentrations of the heavy metals cadmium (3 mg kg(-1)) and chromium (115 mg kg(-1)). this strain was able to grow on complex (luria bertani) medium containing high concentrations of cadmium ion (up to 4 mm). additionally, it could remove up to 80% of the dissolved ions but only after reaching stationary growth phase. strain cd02 also tolerated high concentrations of other heavy metals such as chr ... | 2007 | 17447550 |
surface charge influences enterococcal prevalence in mixed-species biofilms. | to study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. | 2007 | 17448160 |
isolation and characterization of diuron-degrading bacteria from lotic surface water. | the bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (ttge) of 16 s rdna gene v1-v3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. the ttge fingerprints revealed that diuron had a strong impact on bacterial community structure and ... | 2007 | 17450392 |
stenotrophomonas maltophilia infection of the central venous catheter and lysis of an old thrombosis in a post-bone marrow transplantation child: implications in the fibrinolytic process. | in this report, we describe an 11-y-old girl who developed jugular venous thrombosis after allogeneic bone marrow transplantation for lymphoma and then experienced dissolution of the thrombosis following catheter-related stenothrophomonas maltophilia bactearemia. the lysis of the old thrombosis around the central venous catheter suggested a local fibrinolytic activity of s. maltophilia. the global fibrinolytic capacity (gfc) was also tested in vitro by using s. maltophilia cultures obtained from ... | 2007 | 17454909 |
successfully treated nosocomial stenotrophomonas maltophilia bacteremia following desensitization to trimethoprim-sulfamethoxazole. | stenotrophomonas maltophilia has emerged as an important cause of morbidity and mortality in hospitalized patients. because trimethoprim-sulfamethoxazole (tmp-smx) remains the most effective drug for the treatment of s. maltophilia infections, desensitization should be considered in patients with hypersensitivity to tmp-smx. | 2007 | 17458682 |
selection for biocontrol bacteria antagonistic toward rosellinia necatrix by enrichment of competitive avocado root tip colonizers. | biological control of soil-borne pathogens is frequently based on the application of antagonistic microorganisms selected solely for their ability to produce in vitro antifungal factors. the aim of this work was to select bacteria that efficiently colonize the roots of avocado plants and display antagonism towards rosellinia necatrix, the causal agent of avocado white root rot. a high frequency of antagonistic strains (ten isolates, 24.4%) was obtained using a novel procedure based on the select ... | 2007 | 17467245 |
diffusible signal factor-dependent cell-cell signaling and virulence in the nosocomial pathogen stenotrophomonas maltophilia. | the genome of stenotrophomonas maltophilia encodes a cell-cell signaling system that is highly related to the diffusible signal factor (dsf)-dependent system of the phytopathogen xanthomonas campestris. here we show that in s. maltophilia, dsf signaling controls factors contributing to the virulence and antibiotic resistance of this important nosocomial pathogen. | 2007 | 17468254 |
disseminated cutaneous fusarium moniliforme infections in a leukemic child. | a 5-year-old boy had a 10-month remission of acute lymphocytic leukemia (all) after chemotherapy. re-induction chemotherapy was performed for relapse of all. thereafter, he suffered from an episode of neutropenic fever with pneumonia. one week following control of the condition with antibiotics, a 1 x 1-cm, red, painful nodule appeared on the left thigh, which was initially suspected to be pseudomonas infection. parenteral ceftazidime and amikacin were administered, but persistent high fever, mi ... | 2007 | 17472677 |
clinical problem-solving. the drenched doctor - a 55-year-old male physician was seen in august because of a 1-week history of fever and night sweats. | 2007 | 17476014 | |
biodegradation and decolourization of anaerobically treated distillery spent wash by a novel bacterial consortium. | the aim of this study was to isolate microorganisms capable of decolourizing and degrading anaerobically treated distillery spent wash. a bacterial consortium dmc comprising of three bacterial cultures was selected on the basis of rapid effluent decolourization and degradation, which exhibited 67 +/- 2% decolourization within 24 h and 51 +/- 2% chemical oxygen demand reduction within 72 h when incubated at 37 degrees c under static condition in effluent supplemented with 0.5% glucose, 0.1% kh(2) ... | 2007 | 16473005 |
decontamination effects of low-temperature plasma generated by corona discharge. part ii: new insights. | the second part of our paper presents the results of experiments with the decontamination of surfaces by low-temperature plasma generated by corona discharge in air at atmospheric pressure. a simple device is described and the effects of the corona discharge on model microorganisms, viz. the yeast candida albicans, gram-negative bacteria escherichia coli, enterobacter aerogenes, neisseria sicca, stenotrophomonas maltophilia, gram-positive bacteria deinococcus radiodurans, enterococcus faecium, s ... | 2007 | 18225640 |
clinical aspects and risk factors of nosocomial stenotrophomonas maltophilia bacteremia episodes in a turkish intensive care unit. | stenotrophomonas maltophilia is an important nosocomial pathogen with increasing frequency in recent years, especially in immunocompromised and clinically debilitated patients. the aim of this study is to describe the characteristics of 35 episodes of s. maltophilia bacteremia at celal bayar university hospital in turkey over a 3-year period from january 2003 to december 2005. cases were identified with microbiology laboratory records and clinical data were collected from the medical record of e ... | 2007 | 18230546 |
[invasive aspergillosis in an immunocompromised patient: clinical and therapeutic considerations]. | the paper describes a case report of a young female with invasive aspergillosis diagnosed after brief treatment with high-dose steroids for autoimmune thrombocytopenia. early diagnosis of invasive aspergillosis was made with cultures of tracheoaspirates and bronchoalveolar lavage and was confirmed with a transbronchial biopsy. after initial ineffective treatment with liposomal amphotericin b and dissemination from pulmonary to central nervous system involvement, treatment was switched to a combi ... | 2007 | 18162738 |
stenotrophomonas maltophilia and mycobacterium chelonae coinfection of the extraocular scleral buckle explant. | to report a very unusual form of coinfection of the extraocular scleral buckle explant by stenotrophomonas maltophilia and mycobacterium chelonae. | 2007 | 18085488 |
stenotrophomonas maltophilia: changing spectrum of a serious bacterial pathogen in patients with cancer. | stenotrophomonas maltophilia colonization/infection in patients with cancer has significantly increased over the past 2 decades. patients with prolonged neutropenia, exposure to broad-spectrum antibiotics, and those requiring mechanical ventilation have higher risk of infection. these micro-organisms are intrinsically resistant to carbapenems, and exposure to these agents has been linked to selection of s. maltophilia. recently, these infections are being documented in patients without tradition ... | 2007 | 18190323 |
biofilm formation by stenotrophomonas maltophilia isolates from device-associated nosocomial infections. | medical devices are often colonized by bacteria which may cause severe infections. the aim of this work was to evaluate biofilm formation by s. maltophilia isolates from device-associated nosocomial infections. the 13 local isolates exhibited different capacities of biofilm formation on hydrophilic and hydrophobic surfaces. all isolates formed strong biofilms in polystyrene microplates, while strong, moderate or weak biofilms were detected in borosilicate (bs) or polypropylene (pp) tubes. the pr ... | 2007 | 18390153 |
[monosaccharide composition of exopolymer complex in thiobacillus thioparus and stenotrophomonas maltophilia]. | the bacteria of thiobacillus thioparus and stenotrophomonas maltophilia are capable to form a thick biofilm complicated as to its structure on the surface of low-carbon steel. this biofilm formation on any surface occurs under the adhesion of the cells of the plankton growth model with the help of synthesis of muciferous exopolymers with adhesive properties. hence the monosaccharide composition of the exopolymer complex in the form of polyol acetates was studied by chromate-mass-spectrum method. ... | 2007 | 18357787 |
[surveillance of antimicrobial resistance among nosocomial gram-negative pathogens from 15 teaching hospitals in china in 2005]. | to investigate the antimicrobial resistance among the nosocomial gram-negative pathogens from 15 teaching hospitals located in different areas in china in 2005. | 2007 | 18167265 |
capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. | the nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. this dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. the values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pi markers and were found comparable with pi of the native compounds. the m ... | 2006 | 17165837 |
site-selective binding of zn(ii) to metallo-beta-lactamase l1 from stenotrophomonas maltophilia. | extended x-ray absorption fine structure studies of the metallo-beta-lactamase l1 from stenotrophomonas maltophilia containing 1 and 2 equiv of zn(ii) and containing 2 equiv of zn(ii) plus hydrolyzed nitrocefin are presented. the data indicate that the first, catalytically dominant metal ion is bound by l1 at the consensus zn1 site. the data further suggest that binding of the first metal helps preorganize the ligands for binding of the second metal ion. the di-zn enzyme displays a well-defined ... | 2006 | 16489411 |
beta-lactam resistance and beta-lactamase expression in clinical stenotrophomonas maltophilia isolates having defined phylogenetic relationships. | to test the hypothesis that stenotrophomonas maltophilia isolates from certain phylogenetic groups have predictable beta-lactamase expression and beta-lactam resistance profiles. | 2006 | 16352734 |
stenotrophomonas maltophilia endophthalmitis after cataract extraction. | to report the clinical course, treatment response, and prognosis of stenotrophomonas maltophilia endophthalmitis following cataract extraction. | 2006 | 16507490 |
evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli. | the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ... | 2006 | 16517878 |
global assessment of the antimicrobial activity of polymyxin b against 54 731 clinical isolates of gram-negative bacilli: report from the sentry antimicrobial surveillance programme (2001-2004). | in total, 54 731 gram-negative bacilli isolated worldwide between 2001 and 2004 from diverse sites of infection were tested for susceptibility to polymyxin b by the broth reference microdilution method, with interpretation of results according to clsi (formerly nccls) guidelines. polymyxin b showed excellent potency and spectrum against 8705 pseudomonas aeruginosa and 2621 acinetobacter spp. isolates (mic50, < or = 1 mg/l and mic90, 2 mg/l for both pathogens). polymyxin b resistance rates were s ... | 2006 | 16524407 |
bronchoscopy in ventilator-associated pneumonia: agreement of calibrated loop and serial dilution. | although the serial dilution technique for quantitative culture of bronchoalveolar fluid is considered to be the gold standard for the diagnosis of ventilator-associated pneumonia, it is more labor intensive than the calibrated loop technique. | 2006 | 16528014 |
genetic diversity of phenazine- and pyoluteorin-producing pseudomonads isolated from green pepper rhizosphere. | the genetic diversity among indigenous phenazine-1-carboxylic acid (pca)-producing and pyoluteorin (plt)-producing isolates of pseudomonads screened from green pepper rhizosphere was exploited in this study. a total of 48 bacterium isolates producing one or both of these antibiotics were screened from green pepper rhizosphere in diverse regions in china. among these isolates, 45 could produce pca, 3 could produce both pca and plt, and none could produce plt only. based on the restriction pattern ... | 2006 | 16395554 |
stenotrophomonas koreensis sp. nov., isolated from compost in south korea. | a gram-negative, rod-shaped, non-spore-forming bacterium (tr6-01t) was isolated from compost near daejeon city in south korea. on the basis of 16s rrna gene sequence similarity, strain tr6-01t was shown to belong to the class gammaproteobacteria, related to stenotrophomonas acidaminiphila (97.1 %) and stenotrophomonas maltophilia (96.9 %); the phylogenetic distance from any other established species within the genus stenotrophomonas was less than 97.0 %. phenotypic and chemotaxonomic data (major ... | 2006 | 16403870 |
microbiology of airway disease in a cohort of patients with cystic fibrosis. | recent reports document an increasing incidence of new gram-negative pathogens such as stenotrophomonas maltophilia and alcaligenes xylosoxidans isolated from patients with cystic fibrosis, along with an increase in common gram-negative pathogens such as pseudomonas aeruginosa and burkholderia cepacia complex. furthermore, the increase in multidrug-resistance of such organisms makes the therapeutic management of these patients more problematic. therefore, careful isolation and identification, an ... | 2006 | 16405721 |
nosocomial pneumonia : rationalizing the approach to empirical therapy. | nosocomial pneumonia or hospital-acquired pneumonia (hap) causes considerable morbidity and mortality. it is the second most common nosocomial infection and the leading cause of death from hospital-acquired infections. in 1996 the american thoracic society (ats) published guidelines for empirical therapy of hap. this review focuses on the literature that has appeared since the ats statement. early diagnosis of hap and its etiology is crucial in guiding empirical therapy. since 1996, it has becom ... | 2006 | 16409013 |
treatment of cork boiling wastewater using chemical oxidation and biodegradation. | three cultures were enriched from cork boiling wastewater using tannic acid as the selective carbon substrate, at 25 degrees c and ph 7.2, 25 degrees c and ph 4.7 and 50 degrees c and ph 4.7. the enrichment culture obtained at neutral ph was composed of five culturable isolates, whereas from each acidic enrichment two bacterial strains were isolated. mesophilic isolates were gram negative bacteria belonging to the genera klebsiella, pseudomonas, stenotrophomonas and burkholderia. thermophilic is ... | 2006 | 16414098 |
in vitro bactericidal activity of human beta-defensin 3 against multidrug-resistant nosocomial strains. | the antimicrobial activity of human beta-defensin 3 (hbd-3) against multidrug-resistant clinical isolates of staphylococcus aureus, enterococcus faecium, pseudomonas aeruginosa, stenotrophomonas maltophilia, and acinetobacter baumannii was evaluated. a fast bactericidal effect (within 20 min) against all bacterial strains tested was observed. the presence of 20% human serum abolished the bactericidal activity of hbd-3 against gram-negative strains and reduced the activity of the peptide against ... | 2006 | 16436752 |
suspected ventilator-associated pneumonia in cardiac patients admitted to the coronary care unit. | to determine the incidence, risk factors, associated pathogens, and outcome of ventilator-associated pneumonia (vap) in patients admitted to a coronary care unit (ccu). | 2006 | 16438476 |
stenotrophomonas maltophilia bacteremia in burn patients. | stenotrophomonas maltophilia has been increasingly reported as a nosocomial opportunistic pathogen in debilitated patients, including burn patients. there is, however, only one published report in english that discusses s. maltophilia bacteremia in burns. we performed a retrospective chart review and statistical analysis of the incidence, the duration of hospital stays before a diagnosis of bacteremia, antimicrobial susceptibility, prognosis, and mortality risk factors in burn patients. from jan ... | 2006 | 16448762 |
phenotypic characterization of raw milk-associated psychrotrophic bacteria. | among the 68 isolates, selected from 13 raw-milk samples in finland (that originate from farm, truck or silo tanks), 60 (88%) were psychrotrophs. all the isolates were characterized by the determination of their spoilage and phenotypic features: proteolytic and lipolytic activities, the production of lecithinases and hemolytic factors were considered. phenotypic characterization of the isolates was mainly performed with api 20ne and biolog gn2 identification systems; the results were system-depe ... | 2006 | 16459065 |
potentially multidrug-resistant non-fermentative gram-negative pathogens causing nosocomial pneumonia. | owing to its high morbidity and mortality, nosocomial pneumonia represents a particularly serious illness and one of the most frequent complications in ventilated patients admitted to the intensive care unit. gram-negative microorganisms, such as pseudomonas aeruginosa, acinetobacter baumannii and stenotrophomonas maltophilia, are the most relevant pathogens responsible for particularly difficult-to-treat nosocomial pneumonia. the intrinsic resistance of these bacteria to many antimicrobial agen ... | 2006 | 16472990 |
enzymatic c-4 deacetylation of 10-deacetylbaccatin iii. | second-generation paclitaxel analogues that require replacement of the c-4 acetate by other substituents are in development. an enzyme able to specifically remove the c-4 acetate from paclitaxel could simplify preparation of the analogues. several strains were isolated from soil samples that contain enzyme activities able to 4-deacetylate 10-dab (10-deacetylbaccatin iii). selection was made using plates containing 10-dab as the sole carbon source and screening colonies for deacetylation of 10-da ... | 2006 | 16722823 |
purpura fulminans due to stenotrophomonas maltophilia infection in a premature infant. | 2006 | 16732810 |