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genomic analysis of mycobacterium massiliense strain m115, an isolate from human sputum.we report the draft genome sequence of a clinical isolate, strain m115, identified as mycobacterium massiliense, a member of the newly created taxon of mycobacterium abscessus subspecies bolletii comb. nov.201222887681
therapeutic drug monitoring in the treatment of mycobacterium avium complex lung disease.little is known regarding the application of therapeutic drug monitoring for treatment of mycobacterium avium complex (mac) lung disease.201222878282
macrolide treatment for mycobacterium abscessus and mycobacterium massiliense infection and inducible resistance.macrolides, such as clarithromycin (clr) and azithromycin (azm), are frequently the only oral antibiotics that are active against mycobacterium abscessus and m. massiliense infections.201222878281
molecular typing of mycobacterium abscessus based on tandem-repeat polymorphism.a variable-number tandem-repeat (vntr) typing assay for the differentiation of mycobacterium abscessus strains was developed. this assay showed complete reproducibility, locus stability, and a discriminatory power (hunter-gaston discriminatory index [hgdi] of 0.9563) that is superior to that of multilocus sequencing. it is a promising tool for the investigation of mycobacterium abscessus epidemiology and nosocomial outbreaks.201222760048
non-tuberculous mycobacterial infection among lung transplant recipients: a 15-year cohort study.the incidence of infection with non-tuberculous mycobacteria (ntm) after lung transplant is insufficiently defined. data on the impact of ntm infection on lung transplant survival are conflicting.201222676720
genome sequence of the mycobacterium abscessus strain m93.mycobacterium abscessus is a rapid-growing species of nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. we report herein the draft genome sequence of m. abscessus strain m93.201222628507
first case report of chronic pulmonary lung disease caused by mycobacterium abscessus in two immunocompetent patients in saudi arabia.mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterium that is a common water contaminant, and an exclusive opportunistic pathogen in immunosuppressed patients; however, it occasionally causes lung infections in immunocompetent persons as well. we report two cases of chronic pulmonary infection caused by m abscessus in two young migrant workers; they were nonreactive to hiv antigen, without any history of pulmonary disorders and no evidence of any immunocompromized status. bo ...201222588446
activities of moxifloxacin in combination with macrolides against clinical isolates of mycobacterium abscessus and mycobacterium massiliense.infections caused by mycobacterium abscessus and mycobacterium massiliense are on the rise among humans. although macrolides, including clarithromycin (clr) and azithromycin (azm), are key antibiotics for the treatment of m. abscessus and m. massiliense infections, treatment regimens for these infections are still largely undefined. in this study, we evaluated the in vitro, ex vivo, and in vivo activities of moxifloxacin (mxf) in combination with macrolides against clinically isolated m. abscess ...201222564831
non-tuberculous mycobacteria infection: 75 cases.non-tuberculosis mycobacterium is especially seen in aids and non-immunosuppressant patients. this study was designed to evaluate data relating to non-tuberculosis mycobacterium content in patients' sputum for the clinical importance.201222554363
disseminated mycobacterium immunogenum infection presenting with septic shock and skin lesions in a renal transplant recipient.mycobacterium immunogenum is a relatively new species within the mycobacterium chelonae-mycobacterium abscessus group of rapidly growing mycobacteria (rgm). m. immunogenum was first characterized in 2001 and, similar to other rgm, is an ubiquitous environmental organism. this organism has most commonly been implicated in cutaneous infection in both healthy and immunosuppressed patients. to our knowledge, this is the first reported case of septic shock in the setting of disseminated m. immunogenu ...201222548769
resistance mechanisms and drug susceptibility testing of nontuberculous mycobacteria.nontuberculous mycobacteria (ntm) are increasingly recognized as causative agents of opportunistic infections in humans. for most ntm infections the therapy of choice is drug treatment, but treatment regimens differ by species, in particular between slow (e.g. mycobacterium avium complex, mycobacterium kansasii) and rapid growers (e.g. mycobacterium abscessus, mycobacterium fortuitum). in general, drug treatment is long, costly, and often associated with drug-related toxicities; outcome of drug ...201222525524
the use of rpob sequence analysis in the differentiation of mycobacterium abscessus and mycobacterium chelonae: a critical judgement in cystic fibrosis?individuals suffering from fibrocystic disease may acquire non-tuberculous mycobacteria as colonizing or infecting organisms. mycobacterium abscessus is of particular concern because it may be very difficult to eradicate and may mitigate against lung transplantation. however, this species may be difficult to reliably differentiate from the closely related m. chelonae. we have developed a rapid, low-cost, short sequence-based technique to confirm species identity by analysis of a segment of the r ...201222429487
molecular fingerprinting of mycobacterium abscessus strains in a cohort of pediatric cystic fibrosis patients.forty-one mycobacterium abscessus complex isolates from 17 pediatric cystic fibrosis (cf) patients were typed using a novel variable-number tandem repeat (vntr) scheme and an automated repetitive-pcr (rep-pcr) system. both vntr and rep-pcr typing methods differentiate between members of the m. abscessus complex. the isolates from individual patients are indistinguishable, and the data strongly suggest that individual cf patients are persistently infected with one strain and also suggests that di ...201222403419
evaluation of antimicrobial susceptibilities of rapidly growing mycobacteria by sensititre rapmyco panel.this study used sensititre rapmyco to test the activities of amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, linezolid, sulfamehoxazole, tigecycline and tobramycin against 25 clinical isolates of rapidly growing mycobacteria (rgm), including the common disease producing species mycobacterium abscessus, mycobacterium chelonae, mycobacterium fortuitum and mycobacterium peregrinum. analysis of the four different rgm species showed that isolates of m. fortuitum and m. pere ...201222378556
serial ct findings of mycobacterium massiliense pulmonary disease compared with mycobacterium abscessus disease after treatment with antibiotic therapy.to present the serial computed tomographic (ct) findings of lung abnormalities in mycobacterium massiliense pulmonary disease compared with those in mycobacterium abscessus disease.201222371609
pulmonary mycobacterium abscessus: a canary in the cystic fibrosis coalmine.we present a case of pulmonary nontuberculous mycobacterial infection (pntm) with m. abscessus. after exclusion of genetic immune disorders known to cause ntm susceptibility, we found compound heterozygosity of two mutations, f508del and r117h in cftr. the combination of f508del with a hypomorphic cftr mutation can cause a mild cystic fibrosis (cf) phenotype with delayed cf symptoms in adulthood. although the patient was continuously treated for her lung infection by different physicians for mor ...201222366207
antimicrobial susceptibility of nontuberculous mycobacteria from eye infections.to characterize formerly designated atypical or nontuberculous mycobacteria (ntm) associated with eye infections.201222362004
case series of mycobacterium abscessus infections associated with a trigger point injection and epidural block at a rural clinic.the aim of this report is to investigate mycobacterium abscessus infections at a rural clinic and carry out a surveillance program to determine the extent and source of these infections.201222323979
atypical mycobacterium detection in refractory chronic rhinosinusitis.atypical mycobacterium (am) involvement in refractory chronic rhinosinusitis (crs) is sought by some surgeons with customary acid-fast bacilli cultures (afbc) in the operating room (or). we evaluate our experience with am in crs by describing (1) associated risk factors, (2) species identified, and (3) frequency of positive cultures in clinic versus or.201222301103
mycobacterium abscessus: a new antibiotic nightmare.the intrinsic and acquired resistance of mycobacterium abscessus to commonly used antibiotics limits the chemotherapeutic options for infections caused by these mycobacteria. intrinsic resistance is attributed to a combination of the permeability barrier of the complex multilayer cell envelope, drug export systems, antibiotic targets with low affinity and enzymes that neutralize antibiotics in the cytoplasm. to date, acquired resistance has only been observed for aminoglycosides and macrolides, ...201222290346
mycobacterium abscessus granulomatous prostatitis.infectious granulomatous prostatitis is uncommon, and most cases of granulomatous prostatitis are classified as nonspecific granulomatous prostatitis. from 2007 to 2009, 5 patients experienced poor wound healing after radical prostatectomy for prostate cancer at a specialist cancer center. mycobacterium abscessus was cultured from the debridement specimens, and acid-fast-positive bacilli were identified histologically within the prostates. all 180 radical prostatectomy specimens from may 2007 to ...201222261705
hsp65 pcr-restriction analysis (pra) with capillary electrophoresis for species identification and differentiation of mycobacterium kansasii and mycobacterium chelonae-mycobacterium abscessus group.the aim of the present study was to identify and differentiate mycobacterium kansasii and mycobacterium chelonae-mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries.201222240012
the use of a two-gene sequencing approach to accurately distinguish between the species within the mycobacterium abscessus complex and mycobacterium chelonae.mycobacterium abscessus [m. abscessus (sensu lato) or m. abscessus complex] comprises three closely related species: m. abscessus (sensu stricto), hereafter referred to as m. abscessus, m. bolletii and m. massiliense. we describe here an accurate and robust method for distinguishing m. chelonae from m. abscessus, m. bolletii and m. massiliense, using polymerase chain reaction (pcr) and the sequencing of house-keeping gene targets (hsp65 and rpob). sequencing of the soda gene is of little additio ...201222222989
a case of empyema necessitatis caused by mycobacterium abscessus.non-tuberculosis mycobacteria (ntm) have emerged as an important pathogen in lung infection. ntm infection is rarely accompanied by pleural involvement, and empyema necessitatis caused by ntm is very uncommon. we report a very rare case of mycobacterium abscessus pulmonary disease with empyema accompanied by empyema necessitatis. the patient was successfully treated by percutaneous tube drainage of the empyema and empyema necessitatis with aggressive antibiotics treatment and surgical resection.201226029591
epidural abscess caused by mycobacterium abscessus.mycobacterium abscessus is a member of the rapidly growing mycobacterium (rgm). the incidence of mycobacterium abscessus infections has steadily been increasing over the last decade. we report the case of an epidural abscess caused by mycobacterium abscessus. rgm's have infrequently been reported as spinal infections and we found no prior cases reporting m. abscessus as the definitive etiologic agent of an epidural abscess.201223569523
probable disseminated mycobacterium abscessus subspecies bolletii infection in a patient with idiopathic cd4+ t lymphocytopenia: a case report. 201222947084
otitis media and otomastoiditis caused by mycobacterium massiliense (mycobacterium abscessus subsp. bolletii).we describe two patients with otologic infections caused by mycobacterium massiliense (m. abscessus subsp. bolletti) which were identified using erm(41) pcr, 23s rrna, and rpob gene sequence analysis. they were middle-aged adults with underlying otologic diseases and were treated successfully with clarithromycin-based combination regimens for 3 and 9 months, respectively.201222933592
annotated genome sequence of mycobacterium massiliense strain m154, belonging to the recently created taxon mycobacterium abscessus subsp. bolletii comb. nov.mycobacterium massiliense has recently been proposed as a member of mycobacterium abscessus subsp. bolletii comb. nov. strain m154, a clinical isolate from the bronchoalveolar lavage fluid of a malaysian patient presenting with lower respiratory tract infection, was subjected to shotgun dna sequencing with the illumina sequencing technology to obtain whole-genome sequence data for comparison with other genetically related strains within the m. abscessus species complex.201222887675
draft genome sequence of mycobacterium abscessus subsp. bolletii bd(t).mycobacterium abscessus subsp. bolletii is an increasing cause of human pulmonary disease and infections of the skin and soft tissues. consistent reports of human infections indicate that m. bolletii is a highly pathogenic, emerging species of rapidly growing mycobacteria (rgm). here we report the first whole-genome sequence of m. abscessus subsp. bolletii bd(t).201222535937
antibiotic treatment for nontuberculous mycobacteria lung infection in people with cystic fibrosis.nontuberculous mycobacteria are mycobacteria, other than those in the mycobacterium tuberculosis complex, and are commonly found in the environment. nontuberculous mycobacteria species (most commonly mycobacterium avium complex and mycobacterium abscessus) are isolated from the respiratory tract of approximately 5% to 20% of individuals with cystic fibrosis; they can cause lung disease in people with cystic fibrosis leading to more a rapid decline in lung function and even death in certain circu ...201223235684
impaired expression of mapk is associated with the downregulation of tnf-α, il-6, and il-10 in mycobacterium abscessus lung disease.healthy individuals who develop nontuberculous mycobacteria (ntm) lung disease are likely to have specific susceptibility factors which can lead to a ntm infection. the aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (mapk), in mycobacterium abscessus lung disease.201223227067
analysis of the genome of mycobacterium abscessus strain m94 reveals an uncommon cluster of trnas.mycobacterium abscessus is a species of rapidly growing nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. here, we report the annotated genome sequence of m. abscessus strain m94, which showed an unusual cluster of trnas.201223012295
genomic insights into the emerging human pathogen mycobacterium massiliense.mycobacterium massiliense (mycobacterium abscessus group) is an emerging pathogen causing pulmonary disease and skin and soft tissue infections. we report the genome sequence of the type strain ccug 48898.201222965080
progressive dyspnoea following the treatment of mycobacterium abscessus infection in an individual with relapsing granulamatosis with polyangitis (wegener's), complicated by hearing loss requiring cochlear implantation.granulomatosis with polyangitis (wegener's) is a vasculitic disease predominantly affecting the lungs, skin, kidneys, ears, nose and throat. mycobacterium abscessus is an uncommon rapidly growing mycobacterium causing sporadic lung disease. this is the first report of both gpa and mycobacterium abscessus pulmonary disease reported in literature.201222947057
treatment of mycobacterium abscessus: all macrolides are equal, but perhaps some are more equal than others. 201223118083
mycobacterium abscessus lung disease in a patient with previous pulmonary tuberculosis.patients with pre-existing lung damage, such as due to pulmonary tuberculosis (ptb), are susceptible to nontuberculous mycobacteria (ntm) infections. for patients with previous ptb, it is difficult to differentiate ntm lung disease from ptb, especially in tuberculosis (tb) high-burdened countries. here, we report a case of mycobacterium abscessus lung disease with a previous history of ptb. the patient underwent a right upper lobe lobectomy due to disappointing treatment response with anti-tuber ...201223077819
genomic analysis of mycobacterium abscessus strain m139, which has an ambiguous subspecies taxonomic position.mycobacterium abscessus is a ubiquitous, rapidly growing species of nontuberculous mycobacteria that colonizes organic surfaces and is frequently associated with opportunistic infections in humans. we report here the draft genome sequence of mycobacterium abscessus strain m139, which shows genomic features reported to be characteristic of both mycobacterium abscessus subsp. abscessus and mycobacterium abscessus subsp. massiliense.201223045507
impact of molecular diagnosis on treating mendelian susceptibility to mycobacterial diseases.the il-12-ifn-γ axis is critical for immune defense against mycobacterial infections. inherited mutations that affect normal activation of this self-amplifying cytokine reaction lead to increased chances of mycobacterial infections, known as mendelian susceptibility to mycobacterial diseases (msmd). delayed diagnosis and difficulty in identifying pathogenic mycobacteria hinder proper treatment of patients, so the aim of this study was to facilitate the diagnosis of mycobacterial infections in ms ...201223036270
[comparison of clinical manifestations between mycobacterium avium-intracellulare complex and mycobacterium abscessus pulmonary diseases].to compare the clinical manifestations of nontuberculous mycobacterial (ntm) pulmonary diseases caused by mycobacterium avium-intracellulare complex (mac) and mycobacterium abscessus.201223158006
pulmonary sequestration infected with nontuberculous mycobacteria: a report of two cases and literature review.we report two cases of pulmonary sequestration infected with nontuberculous mycobacteria (ntm): mycobacterium avium and mycobacterium abscessus. chest computed tomography showed pneumonic consolidation in the right lower lobe, which received a systemic blood supply from the descending aorta in both patients. video-assisted thoracoscopic surgeries were successfully performed and pathological examinations revealed multiple caseating granulomas. a review of the literature revealed only seven previo ...201223146810
genome sequence of mycobacterium abscessus strain m152.mycobacterium abscessus is an environmental bacterium with increasing clinical relevance. here, we report the annotated whole-genome sequence of m. abscessus strain m152.201223144407
are we overlooking infections owing to non-tuberculous mycobacteria during routine conventional laboratory investigations?a large number of potentially pathogenic non-tuberculous mycobacteria (ntm) encountered in the clinical laboratory makes it necessary to identify their species to ensure appropriate treatment. however, labor-intensive conventional methods of speciation are not used in every laboratory, and hence ntm infections are often ignored. polymerase chain reaction (pcr) restriction analysis (pra) was applied in this study for early identification and speciation of mycobacterial species on 306 cultures of ...201226785625
mycobacterium abscessus glycopeptidolipid prevents respiratory epithelial tlr2 signaling as measured by hβd2 gene expression and il-8 release.mycobacterium abscessus has emerged as an important cause of lung infection, particularly in patients with bronchiectasis. innate immune responses must be highly effective at preventing infection with m. abscessus because it is a ubiquitous environmental saprophyte and normal hosts are not commonly infected. m. abscessus exists as either a glycopeptidolipid (gpl) expressing variant (smooth phenotype) in which gpl masks underlying bioactive cell wall lipids, or as a variant lacking gpl which is i ...201122216191
molecular identification and typing of mycobacterium massiliense isolated from postsurgical infections in brazil.one hundred thirty-one cases of postsurgical infections were reported in southern region of brazil between august 2007 and january 2008. thirty-nine (29.8%) cases were studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility and clonal diversity of rapidly growing mycobacteria isolated in this outbreak.201122230849
Identification and treatment of nontuberculous Mycobacterium sinusitis.The purpose of this study was to identify the incidence of atypical Mycobacterium identified by routine sinus cultures and review the recent literature on management.201122185748
rapid growing mycobacteria and tnf-α blockers: case report of a fatal lung infection with mycobacterium abscessus in a patient treated with infliximab, and literature review.rapid growing mycobacteria pose as a serious emerging threat for patients treated with tnf-α blockers. we report a case of a progressive and fatal pulmonary infection with m. abscessus in a patient previously treated with infliximab for crohn's disease and review seven other previously reported patients. five out of eight patients were female. the patients were treated with either anti-tnf-α antibodies or tnf-α blocking receptor. the diagnosis of rgm came in the first 6 months after initiation o ...201121906433
[Diagnosis and treatment of atypical mycobacterial infections of the respiratory tract].INTRODUCTION: Non tuberculous mycobacteria (NTM), unlike tuberculous mycobacteria, are not strictly human pathogens. The diagnosis of infection and the choice of treatment remain difficult. BACKGROUND: Evidence of a NTM in a pulmonary sample is not synonymous with infection. The diagnosis depends on the association of clinical, radiological and microbiological factors. If a NTM is isolated from a respiratory sample, the probability of infection depends on the species. The main NTMs responsible f ...201122152937
Antibacterial activities of dendritic amphiphiles against nontuberculous mycobacteria.The anti-mycobacterial activities of nine series of dicarboxyl and tricarboxyl dendritic amphiphiles with one alkyl, two alkyl, and cholestanyl tails against Mycobacterium abscessus, Mycobacterium avium, Mycobacterium chelonae, Mycobacterium marinum and Mycobacterium smegmatis have been measured. The dendritic amphiphiles overcame the limited aqueous solubility of natural long-chain fatty acids, alcohols, and amines to enable profiling the susceptibilities of the different mycobacterial species ...201122209468
multifocal infection of mycobacterium other than tuberculosis mimicking a soft tissue tumor of the extremity.mycobacterium other than tuberculosis infections rarely develop in healthy individuals, but direct inoculation such as contaminated acupuncture can cause mycobacteriosis even in an immunocompetent host. a 56-year-old woman gradually developed pain on the anterior aspect of the left knee and the distal thigh after hiking without trauma. she received acupuncture for 3 consecutive days on the bilateral knees at the suprapatellar and infrapopliteal areas. after acupuncture, mild localized heat and p ...201122146217
conditional gene expression in mycobacterium abscessus.mycobacterium abscessus is an emerging human pathogen responsible for lung infections, skin and soft-tissue infections and disseminated infections in immunocompromised patients. it may exist either as a smooth (s) or rough (r) morphotype, the latter being associated with increased pathogenicity in various models. genetic tools for homologous recombination and conditional gene expression are desperately needed to allow the study of m. abscessus virulence. however, descriptions of knock-out (ko) m ...201122195042
clinical significance of mycobacterium abscessus isolates at a medical center in northern taiwan. 201121855429
overexpression of proinflammatory tlr-2-signalling lipoproteins in hypervirulent mycobacterial variants.changes in the cell envelope composition of mycobacteria cause major changes in cytokine profiles of infected antigen presenting cells. we describe here the modulation of inflammatory responses by mycobacterium abscessus, an emerging pathogen in cystic fibrosis. m. abscessus is able to switch from a smooth (s) to a rough (r) morphotype by the loss of a surface glycopeptidolipid. r variants are associated with severe clinical forms and a 'hyper-proinflammatory' response in ex vivo and in vivo mod ...201121143571
disseminated mycobacterium abscessus infection and showerheads, taiwan. 201122099106
evaluation of infections in the lung transplant patient.purpose of review: infections in lung transplant recipients (ltrs) are a serious complication that is associated with high mortality. early and accurate diagnosis is critical in the management of these infections in order to achieve improved outcomes. this review focuses on studies published in the last 2 years related to the evaluation and management of infections following lung transplantation. recent findings: valganciclovir is well tolerated and effective for long-term cytomegalovirus prophy ...201122123666
[disseminated infection due to mycobacterium abscessus in an hiv-infected patient]. 201121981938
genetic analysis of new 16s rrna mutations conferring aminoglycoside resistance in mycobacterium abscessus.we studied the development and fitness cost of 2-deoxystreptamine aminoglycoside resistance of mycobacterium abscessus.201121652621
azithromycin blocks autophagy and may predispose cystic fibrosis patients to mycobacterial infection.azithromycin is a potent macrolide antibiotic with poorly understood antiinflammatory properties. long-term use of azithromycin in patients with chronic inflammatory lung diseases, such as cystic fibrosis (cf), results in improved outcomes. paradoxically, a recent study reported that azithromycin use in patients with cf is associated with increased infection with nontuberculous mycobacteria (ntm). here, we confirm that long-term azithromycin use by adults with cf is associated with the developme ...201121804191
mycobacterium abscessus outbreak after rhytidectomies performed in an outpatient surgery center. 201121788807
[inhaled antibiotic therapy in cystic fibrosis].cystic fibrosis is the most frequent fatal genetically-transmitted disease among caucasians. chronic bronchial infection, especially by pseudomonas aeruginosa, is the main cause of morbidity and mortality in this disease. aerosolized antibiotic therapy achieves high drug concentrations in the airway with low toxicity, allowing chronic use. currently, two antibiotics have been approved for inhalation therapy, tobramycin inhalation solution and colistimethate sodium aerosol. there is less evidence ...201121703474
diagnosis of pulmonary disease caused by mycobacterium abscessus: a case report.mycobacterium abscessus (m. abscessus) is a non-tuberculous mycobacterium and an important emerging pathogen causing skin, soft tissue and pulmonary infections. the case of a 59-year-old man with a history of pulmonary tuberculosis (tb) and current pulmonary infection due to m. abscessus, complicated with pneumocardial disease and bronchiectasis, is described. zhiel-neelsen stain and acid lowenstein-jensen culture were both positive for acid-fast bacillus. the patient was initially misdiagnosed ...201121819731
mycobacterium abscessus bacteremia in an immunocompetent patient following a coronary artery bypass graft.sternal osteomyelitis with bacteremia due to mycobacterium abscessus is rarely seen in immunocompetent hosts. routine pyogenic cultures in these cases are often negative causing a delay in diagnosis and treatment. clinicians and microbiologists should rule out the possibility of infection due to nontuberculous mycobacteria while managing cases of nonhealing culture-negative wounds with conventional antibiotic therapy. we report a case of bacteremia secondary to a nonhealing sternal wound due to ...201121716760
an outbreak of cutaneous infection due to mycobacterium abscessus associated to mesotherapy.in february 2009 an outbreak of subcutaneous abscesses due to mycobacterium abscessus was detected in spain which affected healthy women who had undergone mesotherapy procedures in an aesthetic clinic.201121684045
[a case of pulmonary mycobacterium abscessus infection with asbestosis in a patient given multidisciplinary treatment].a 68-year-old man consulted our hospital because of a giant cavity in the upper lobe of the right lung, which was detected in a medical examination for asbestosis. mycobacterium abscessus was cultured from his sputum in 2 separate cultures. therefore we diagnosed pulmonary mycobacterium abscessus infection and prescribed amikacin, imipenem/cilastatin, and clarithromycin. after 2 months, lobectomy of the upper lobe of the right lung was performed. the 3 antibiotics were continued for another 6 mo ...201121688648
Detection of Mycobacterium abscessus from blood cultures during treatment of interstitial pneumonia: a case study.A 76-year-old male diagnosed with interstitial pneumonia in December 2002 was treated with a steroid in a nearby hospital. Exacerbation of infectious pneumonitis and interstitial pneumonia required complementary inpatient treatment in August 2007. Although polymerase chain reaction examination of expectorated sputa revealed the absence of Mycobacterium tuberculosis, M. avium, and M. intracellulare on admission, nontuberculous M. abscessus was detected in the routine blood cultures. Taken togethe ...201122111302
septic arthritis caused by mycobacterium fortuitum and mycobacterium abscessus in a prosthetic knee joint: case report and review of literature.nontuberculous mycobacterium (ntm) is an infrequent cause of prosthetic knee joint infections. simultaneous infection with different ntm species in a prosthetic knee joint has not been previously reported. a case of prosthetic knee joint infection caused by mycobacterium abscessus and m. fortuitum is described in this report. the patient was successfully treated with adequate antibiotics and surgery. the clinical features of sixteen previously reported cases of prosthetic knee joint infection ca ...201121963746
successfully treated mycobacterium abscessus mastitis: a rare cause of breast masses.rapidly growing mycobacteria are pathogens responsible for cutaneous or subcutaneous infections especially occurring after injection, trauma or surgery. we describe a patient with mycobacterium abscessus mastitis that presented as a mass lesion and haemorrhagical discharge. it was initially diagnosed and treated as fibrocystic disease and non-specific abscess. full recovery was obtained with combination therapy of clarithromycin, linezolid and amikacin without surgical debridement followed by se ...201122120809
Comparative study with two different enrichments in the culture media used in the disinfectant efficacy assay.Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide evidence of efficacy of sterilizing and disinfectant products, used for semi critical medical devices, against the Mycobacterium bovis BCG Moreau and Mycobacterium abscessus subsp. bolletii INCQS 0059 ...201122197720
impaired health-related quality of life in pulmonary nontuberculous mycobacterial disease.the impact of pulmonary nontuberculous mycobacterial (pntm) disease on health-related quality of life (hrql) has not been quantified.201121868209
Mycobacterium abscessus activates the NLRP3 inflammasome via Dectin-1-Syk and p62/SQSTM1.Numerous atypical mycobacteria, including Mycobacterium abscessus (Mabc), cause nontuberculous mycobacterial infections, which present a serious public health threat. Inflammasome activation is involved in host defense and the pathogenesis of autoimmune diseases. However, inflammasome activation has not been widely characterized in human macrophages infected with atypical mycobacteria. Here, we demonstrate that Mabc robustly activates the nucleotide binding and oligomerization domain-like recept ...201121876553
expanding the mycobacterial diversity of metalworking fluids (mwfs): evidence showing mwf colonization by mycobacterium abscessus.nontuberculous mycobacteria (ntm) have been associated with hypersensitivity pneumonitis in machinists. only two species of ntm, namely mycobacterium immunogenum and mycobacterium chelonae, have been reported thus far to have the ability to colonize contaminated metalworking fluids (mwfs). here, we report, for the first time, the presence and characterization (phenotypic and genotypic) of a third species, mycobacterium abscessus, colonizing these harsh alkaline machining fluids. two mycobacteriu ...201122092754
the cost of medical management of pulmonary nontuberculous mycobacterial disease in ontario, canada.treatment of pulmonary nontuberculous mycobacterial (ntm) infection is complex, requiring multiple antibiotics and a prolonged treatment course. we determined the monthly cost of treating patients with pulmonary ntm infections in our clinic, a tertiary care centre in toronto, ontario, canada. we reviewed records of a single clinic at the university health network (toronto) for all patients with pulmonary ntm isolates. pharmacological and nonpharmacological treatment costs were calculated using a ...201120817704
clinical significance of differentiation of mycobacterium massiliense from mycobacterium abscessus.mycobacterium massiliense has been recognized as a separate species from mycobacterium abscessus; however, little is known regarding the clinical impact of this differentiation.201120833823
outcomes in patients with mycobacterium abscessus pulmonary disease treated with long-term injectable drugs.the ats (american thoracic society) has recommended periodic administration of multidrug therapy, including a macrolide and one or more parenteral agents or a combination of parenteral agents, over 2-4 months, for treatment of mycobacterium abscessus pulmonary disease. however, there is little hard evidence supporting these guidelines, and treatment outcomes have not yet been reported.201121211956
the clinical course of rapidly growing nontuberculous mycobacterial peritoneal dialysis infections in asians: a case series and literature review.peritoneal dialysis (pd)-related infections due to rapidly growing nontuberculous mycobacterium (rgntm) are rare in asians and have variable clinical outcomes.201121272129
clinical and microbiologic outcomes in patients receiving treatment for mycobacterium abscessus pulmonary disease.mycobacterium abscessus can produce a chronic pulmonary infection for which little is known regarding optimal treatment and long-term outcomes.201121292659
the talking mycobacterium abscessus blues. 201121292660
deletion of the mmpl4b gene in the mycobacterium abscessus glycopeptidolipid biosynthetic pathway results in loss of surface colonization capability, but enhanced ability to replicate in human macrophages and stimulate their innate immune response.mycobacterium abscessus is considered to be the most virulent of the rapidly growing mycobacteria. generation of bacterial gene knockout mutants has been a useful tool for studying factors that contribute to virulence of pathogenic bacteria. until recently, the optimal genetic approach to generation of m. abscessus gene knockout mutants was not clear. based on the recent identification of genetic recombineering as the preferred approach, a m. abscessus mutant was generated in which the gene mmpl ...201121292749
immunohistochemical and taqman real-time pcr detection of mycobacterial infections in fish.real-time pcr and immunohistochemistry (ihc) assays were developed to detect fish mycobacterial infections at the genus level, based on the rna polymerase ß subunit (rpob) gene and polyclonal anti-mycobacterium rabbit serum, respectively. the pcr assay positively identified a number of pathogenic mycobacteria including mycobacterium abscessus, m. avium ssp. avium, m. bohemicum, m. chelonae ssp. chelonae, m. farcinogenes, m. flavescens, m. fortuitum ssp. fortuitum, m. gastri, m. gordonae, m. immu ...201121306590
importance of antibiotic penetration in the antimicrobial resistance of biofilm formed by non-pigmented rapidly growing mycobacteria against amikacin, ciprofloxacin and clarithromycin.to study the resistance of biofilms developed by non-pigmented rapidly growing mycobacteria (nprgm) against amikacin, ciprofloxacin and clarithromycin in an in vitro model using clinical strains of different species.201121333405
strategies to improve outcome of drug treatment for mycobacterium abscessus pulmonary disease. 201121364074
development of multiplex pcr assays based on the 16s-23s rrna internal transcribed spacer for the detection of clinically relevant nontuberculous mycobacteria.to accelerate the identification and differentiation of clinically relevant nontuberculous mycobacteria (ntm) with two sets of multiplex pcr (mpcr) targeting the 16s-23s rrna internal transcribed spacer (its) region for timely patient management.201121395629
[mycobacterium abscessus pulmonary infection treated with tigecyclin-amikacin and cefoxitin in a diabetic patient]. 201121435808
identification of antimicrobial activity among fda-approved drugs for combating mycobacterium abscessus and mycobacterium chelonae.objectives rapidly growing mycobacteria have long been neglected in drug discovery efforts and this neglect is reflected in the paucity of therapeutic options available for diseases resulting from these infections. the purpose of this work is to identify new candidate drugs for treating non-tuberculous mycobacteria (ntm) by testing fda-approved drugs for antimicrobial activity against mycobacterium abscessus and mycobacterium chelonae, two emerging ntm drug-resistant pathogens. methods in this s ...201121486854
cutaneous mycobacterium abscessus infection associated with mesotherapy injection.non-tuberculous mycobacterial skin infections have an increasing incidence. in immunocompetent patients, they usually follow local trauma. we present a case of cutaneous mycobacterium abscessus infection following mesotherapy. the lesions were successfully treated with a combination of clarithromycin, ciprofloxacin, and doxycycline. atypical mycobacterial infection should be suspected in patients who develop late-onset skin and soft tissue infection after cutaneous injury, injection, and surgica ...201121487459
demonstration of cord formation by rough mycobacterium abscessus variants: implications for the clinical microbiology laboratory.in low-income countries some infections caused by nontuberculous mycobacteria are misdiagnosed as multidrug-resistant tuberculosis. in most of these settings the observation of microscopic cords is the only technique used to identify mycobacterium tuberculosis in the laboratory. in this article we definitively demonstrate that mycobacterium abscessus, an emerging pulmonary pathogen, also forms microscopic cords.201121490192
diagnostic value of an enzyme-linked immunospot assay for interferon-γ in cutaneous tuberculosis.the aim of this study was to evaluate the diagnostic performance of an enzyme-linked immunospot (elispot) assay (t-spot.tb; oxford immunotec, oxford, uk) for interferon-γ in patients with suspected cutaneous tuberculosis (tb). from march 2007 to june 2010, a total of 45 patients with suspected cutaneous tb were enrolled. data on clinical characteristics of the patients and conventional laboratory results were collected, and blood samples were obtained for elispot assay. ten subjects (22.2%) had ...201121513844
disseminated mycobacterium abscessus infection in an aids patient. 201121552751
assessment of infection risks prior to lung transplantation.purpose of this review: infections are major causes of morbidity and mortality after lung transplantation. pretransplant evaluation can identify patients at risk of infectious complications and guide prophylactic strategies post transplantation. this review focuses on studies published from 2006 to the present that relate to the assessment of risk of infection prior to lung transplantation. recent findings: pretransplant airways colonization with pseudomonas, burkholderia, nontuberculosis mycoba ...201020847696
the use of quaternary ammonium disinfectants selects for persisters at high frequency from some species of non-tuberculous mycobacteria and may be associated with outbreaks of soft tissue infections.non-tuberculous mycobacteria (ntm) are increasingly important as opportunistic infections after major and minor surgical procedures, likely because they are ubiquitous and not effectively killed by many commonly used disinfectants. outbreaks of soft tissue infections with ntm appeared related to the use of commercial disinfectants based on quaternary ammonium compounds (qacs).201020926395
clarithromycin and amikacin vs. clarithromycin and moxifloxacin for the treatment of post-acupuncture cutaneous infections due to mycobacterium abscessus: a prospective observational study.clin microbiol infect abstract: an outbreak of post-acupuncture cutaneous infections due to mycobacterium abscessus occurred in ansan, korea, from november 2007 through to may 2008. during this time a prospective, observational, non-randomized study was conducted involving 52 patients that were diagnosed with cutaneous m. abscessus infection. we compared the clinical response between patients treated with clarithromycin plus amikacin regimen and those treated with clarithromycin plus moxifloxaci ...201020946409
in vitro antimicrobial activities of capuramycin analogues against non-tuberculous mycobacteria.to determine antibacterial activity of capuramycin analogues sq997, sq922, sq641 and rks2244 against several non-tuberculous mycobacteria (ntm).201020952419
[a case of pulmonary mycobacterium abscessus disease complicated with esophago-pulmonary fistula causing repeated exacerbation].we report a case of fatal pulmonary infection caused by mycobacterium abscessus in a middle-aged patient with acquired esophago-pulmonary fistula which contributed to repeated exacerbation. acquired benign esophago-pulmonary fistula is uncommon. after a 55-year-old woman was diagnosed with pulmonary mycobacterium avium disease in 1989 and improved with multidrug chemotherapy, she developed pulmonary m. abscessus in 2001. despite multi-drug treatment, she suffered repeated exacerbation and her co ...201020954373
mycobacterium abscessus infection in transplant recipients.mycobacterium abscessus is a ubiquitous, rapidly growing mycobacterium that colonizes organic surfaces. it is a potential pathogen, especially in immunosuppressed patients, including transplant recipients in whom disease can range from localized cutaneous lesions to disseminated infections. the purpose of this study was to describe the 12-year impact from january 1997 to december 2009 of m. abscessus infection among solid organ and bone marrow transplantations performed in adults and children.201020970610
proposal that the two species mycobacterium massiliense and mycobacterium bolletii be reclassified as mycobacterium abscessus subsp. bolletii comb. nov., designation of mycobacterium abscessus subsp. abscessus subsp. nov., and emendation of mycobacterium abscessus.the names 'mycobacterium abscessus subsp. abscessus' and 'm. abscessus subsp. massiliense', proposed by leao et al. 2009, cannot be validly published. the purpose of this note is to provide a description in accordance with the rules of the bacteriological code (1990 revision). moreover, the proposal of the name 'm. abscessus subsp. massiliense' is contrary to the rule 38 and the correct name of this taxon, at the rank of subspecies is m. abscessus subsp. bolletii.201021037035
non-tuberculous mycobacterial infections of the skin: a retrospective study of 29 cases.the incidence of infections caused by non-tuberculous mycobacteria has increased in recent years, due to a rise in dermatological procedures and a greater prevalence of immunosuppression in the general population. this study investigated the clinical and microbiological findings of non-tuberculous mycobacterial skin infections. the study population included 29 patients from whom non-tuberculous mycobacteria were cultured after isolation from skin biopsy materials, cutaneous abscesses or exudates ...201021039785
mycobacterium abscessus corneal ulcer following sutured clear corneal cataract incision. 201021052916
clinical outcome of mycobacterium abscessus infection and antimicrobial susceptibility testing.mycobacterium abscessus is the most resistant and rapidly growing mycobacterium and causes a wide range of clinical infectious diseases. the relationship between antimicrobial susceptibility and clinical outcome needs to be further evaluated.201021075707
diversity of pulsed-field gel electrophoresis patterns of mycobacterium abscessus type 2 clinical isolates.an epidemic of infections by rapidly growing mycobacteria related to surgical procedures between 2004 and 2008 in brazil was caused by a unique strain showing the mycobacterium abscessus type 2 pattern when it was analyzed by the molecular method of pcr-restriction enzyme analysis of the hsp65 gene (pra-hsp65). in order to investigate the diversity of m. abscessus type 2 clinical isolates and to assess whether this epidemic strain was present in specimens from nonsurgical patients, we studied 52 ...201021084521
[pathological study of mycobacterium abscessus keratitis].to characterize the cytopathology, histopathology, immunohistochemistry and ultrastructural organization features of mycobacterium abscessus keratitis.201021092563
the peptidoglycan of mycobacterium abscessus is predominantly cross-linked by l,d-transpeptidases.few therapeutic alternatives remain for the treatment of infections due to multiresistant mycobacterium abscessus. here we show that the peptidoglycans of the "rough" and "smooth" morphotypes contain predominantly 3→3 cross-links generated by l,d-transpeptidases, indicating that these enzymes are attractive targets for the development of efficient drugs.201021097619
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