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glutamine-stimulated amino acid and peptide incorporation in bacteroides melaninogenicus.the uptake of a number of amino acids and dipeptides by cells and spheroplasts of bacteroides melaninogenicus was stimulated by the presence of glutamine; 50 mm glutamine induced maximum uptake of glycine or alanine, and glutamine stimulated the uptake of glycine over a wide concentration range (0.17 to 170 mm). glutamine stimulated the uptake of the dipeptides glycylleucine and glycylproline at significantly faster rates compared with glycine and leucine. the amino acids whose uptake was stimul ...19807204333
antigenic studies of oral and nonoral black-pigmented bacteroides strains.antigens of several oral and nonoral strains of bacteroides asaccharolyticus (proposed classification of oral b. asaccharolyticus, bacteroides gingivalis), bacteroides melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, and b. melaninogenicus subsp. levii were identified in soluble preparations obtained by sonication, autoclaving, and naoh treatment of whole bacterial cells. the sonicate preparations contained the most complete representation of soluble antigens using ...19807216426
susceptibility of bacteroides melaninogenicus to 45 antibiotics.the minimal inhibitory concentrations of 45 antimicrobial agents were determined for 13 strains of bacteroides melaninogenicus. all the strains showed good susceptibility to those agents known to be active against anaerobic gram-negative rods, such as tetracyclines and lincomycins. the lowest mics were observed with lincomycin, clindamycin, methacycline, doxycycline, minocycline, and erythromycin. as expected, aminoglycosides were only poorly active, also some strains showed clear resistance to ...19807363706
sequential vaginal cultures from normal young women.vaginal specimens were collected two to three times a week for 1 month from seven nurses. a total of 65 specimens were collected. each sample consisted of three swabs and a saline wash. semiquantitation of anaerobic and aerobic bacteria, mycoplasma and ureaplasma, and yeast was performed. numerous species were recovered in each specimen; at least 37 species were isolated. lactobacilli, corynebacterium, ureaplasma, mycoplasma, bacteroides melaninogenicus, and candida albicans, when present, tende ...19807381013
modulation of immunoreactivity to periodontal disease-associated microorganisms during pregnancy.the lymphocyte blastogenic response to a panel of antigens and mitogens was assessed in a group of 20 women throughout their pregnancy. in addition, a group of five nonpregnant women was monitored simultaneously to identify variations in response to the same stimulants. the stimulants included orally associated bacterial antigens (streptococcus sanguis, actinomyces viscosus, bacteroides asaccharolyticus, bacteroides melaninogenicus subsp. intermedius, bacteroides [capnocytophaga] ochraceus, and ...19807399691
anaerobic breast abscesses. 197984192
ultrastructure of bacteroides species: bacteroides asaccharolyticus, bacteroides fragilis, bacteroides melaninogenicus subspecies melaninogenicus, and b. melaninogenicus subspecies intermedius.representative strains of two subspecies of bacteroides melaninogenicus (subspecies melaninogenicus and subspecies intermedius) and bacteroides asaccharolyticus as well as b. asaccharolyticus strain 536b isolated from a human perirectal abscess and bacteroides fragilis atcc 25285 were examined by glutaraldehyde-osmium fixation, ruthenium red fixation and staining, and thorium hydroxyde staining as well as by the physical preparative techniques of critical point drying--transmission electron micr ...197986590
occurrence of anaerobic bacteria in diseases of the dog and cat.a survey for anaerobic bacteria was conducted in 314 clinical specimens from dogs and cats. a total of 187 anaerobic isolates in pure and mixed culture were isolated from 111 of the specimens that contained anaerobic bacteria. common isolated included actinomyces (9.1%), clostridium perfringens (19.3%), other clostridium spp (11.2%), peptostreptococcus anaerobius (7.5%), bacteroides melaninogenicus (13.4%), other bacteroides spp (17.6%), and fusobacterium necrophorum (5.3%). anaerobic bacteria w ...1979224739
the bacteriology of perimandibular space infections.the bacteriology of 21 perimandibular clised-space infections was studied prospectively using optimal bacteriological techniques to collect, transport, and process specimens. there was an average of six microbial species per specimen, including approximately four anaerobes and two aerobes. the predominant aerobes were alpha-hemolytic and non-hemolytic streptococci; the predominant anaerobes were peptostreptococci, bacteroides melaninogenicus, and fusobacterium nucleatum. these findings indicate ...1979286029
a study of the bacteria associated with advancing periodontitis in man.samples of apical plaque were taken by means of an anaerobic gas-flushed syringe from 21 sites in eight patients. the samples were anaerobically dispersed, diluted and plated and incubated in an atmosphere of 80% n2, 10% h2 and 10% co2 for 7-21 days. all colonies on plates containing 20-50 isolates were picked, repeatedly restreaked, characterized and identified where possible by a probabilistic computer identification program. the sites were divided into four groups on the basis of clinical fea ...1979294457
the fetid foot: lower-extremity infections in patients with diabetes mellitus.infections of the lower extremities in patients with diabetes mellitus have been attributed to staphylococcus aureus and other facultatively anaerobic bacteria. however, a review of 30 consecutive diabetics who required surgery for lower-extremity infections revealed that 17 had mixed infections due to both obligate and facultative anaerobes; only six had infections due to s. aureus. mixed infections often had characteristics of anaerobic suppuration and responded poorly to therapy. patients wit ...1979318225
cefamandole therapy in anaerobic infections.thirty-one adult patients with infections due to anaerobic bacteria were treated with cefamandole. bacteroides fragilis group (17) and bacteroides melaninogenicus (13) were the most frequent anaerobes isolated. duration of therapy varied from 2 to 49 days. results were judged satisfactory in 26 cases, and unsatisfactory in 1 case. four cases could not be evaluated. adverse reactions occurred in 16 patients and included positive direct coombs' test without hemolysis, transient liver function abno ...1979380458
clindamycin in treatment of aspiration pneumonia in children.twenty-eight patients with anaerobic pleuropulmonary infections were treated with clindamycin alone or clindamycin with gentamicin. sixteen of the patients presented with pneumonitis, nine with necrotizing pneumonia, and three with lung abscesses. the average length of treatment was 13.8 days, and the duration of temperature after initiation of therapy was 3.1 days. the predominant isolates were anaerobic gram-positive cocci (23 isolates), bacteroides melaninogenicus (14), bacteroides fragilis ( ...1979380459
antibacterial properties of lidocaine.the inhibitory effect of lidocaine and lidocaine plus methylparaben as a preservative was studied with 25 strains of bacteria. the tested strains were mixed with each topical anesthetic preparation, and quantitative cultures were performed on aspirates at 0, 30, 60, and 120 minutes. results were compared to counts obtained in a lactated ringer's solution, which were sampled at the same intervals. lidocaine (1 percent solution) reduced quantitative counts after 120 minutes of contact by over one ...1979446170
capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections.combinations of bacteria isolated from the root canals of teeth with necrotic pulps and periapical bone destruction were tested for their capacity to induce abscess formation and transmissible infections when inoculated subcutaneously into guinea pigs. transmissible infections could be induced with combinations obtained from teeth with purulent apical inflammation, but not with combinations from symptomless teeth with chronic apical inflammation. all combinations which gave transmissible infecti ...1979489126
obligate anaerobes in clinical veterinary practice.clinical specimens obtained from domestic animals were examined to determine the relative prevalence of obligate anaerobic bacteria and the species represented. of 3,167 samples cultured anaerobically as well as aerobically, 2,234 were bacteriologically positive. of these positive samples, 583 (26%) contained species of obligate anaerobic bacteria in a total of 641 isolates. most positive samples contained anaerobes admixed with aerobic species, although 6% of such samples yielded pure cultures ...1979511987
surface antigens as virulence factors in infection with bacteroides fragilis.organisms of the genus bacteroides represent the major group of obligate anaerobes involved in human infections. bacteroides usually cause either bacteremia or localized abscesses. of the numerous species of bacteroides, bacteroides fragilis is the single most frequent clinical isolate. b. fragilis and bacteroides melaninogenicus have chemically incomplete lipopolysaccharides as compared with the lipopolysaccharides (endotoxins) of aerobic bacteria, and the lipopolysaccharides of bacteroides lac ...1979549184
bacteriology and therapy of lung abscess in children.the bacteriology and clinical findings of ten pediatric patients with lung abscess are presented. bacteriologic data were based on percutaneous transtracheal aspiration obtained before initiation of antimicrobial therapy. anaerobic bacteria were present in all ten patients; in nine they were mixed with aerobic bacteria. the most frequent isolates were peptostreptococcus (8), peptococcus (5), bacteroides melaninogenicus (6), and bacteroides fragilis (3). the aerobic isolates most frequently recov ...1979758385
longitudinal study of experimentally induced periodontal disease in macaca arctoides: relationship between microflora and alveolar bone loss.macaca arctoides monkeys develop periodontal disease, and they harbor a periodontopathic indigenous flora largely similar to that of humans. this study showed that various haemophilus isolates and h2o2-splitting asaccharolytic bacteroides melaninogenicus strains constituted major segments of the monkey periodontal microflora. these organisms have not been previously identified among human isolates. furthermore, the present data revealed that asaccharolytic b. melaninogenicus strains increased in ...197933902
chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria.lactamase production. reacteroides melaniongenicus, 14197934624
serological identification of bacteroides species by an enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of bacteroides with the edta-released outer-membrane complex from 29 characterised strains of bacteroides species. with only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the heterologous type species. cross-reactivity between heterologous species and antiserum was only significan ...197934633
[phlegmon of the fore arm succeeding to a dog-bite : respective role of the miscellaneous bacteria involved in this mixed infection (author's transl)].the authors report a case of a severe mixed infection (phlegmon of the fore arm) succeeding to a dog bite. a complex flora has been isolated from this phlegmom : pasteurella multocida, pasteurella pneumotropica, streptococcus mitis, actinomyces sp., bacteroides melaninogenicus, and a gram- bacteria related to group ii j. the pathogeny of such diseases is discussed, dwelling in one hand on the part of synergistic development of these various germs and in the other hand on the part played by saliv ...197934886
[the bacterial flora of preputial space].the bacterial flora of the preputial space of 210 healthy males (43 children aged between 2 and 11 years, 137 males between 12 and 60 years and 30 men over 60 years) was determined by smears and cultueres from glans penis, sulcus coronarius and the adjacent prepuce. the results were grouped according to various criteria, e.g. glans covered or uncovered and age of males. differences in the distribution of germs could be found in relationship to age. in the case of an uncovered glans penis the pre ...197935486
antibiotic susceptibility of anaerobic bacteria with special reference to bacteroides fragilis.it was shown that recent swedish clinical isolates of anaerobic bacteria are susceptible to many antibiotics by the agar dilution method with the exception of the bacteroides group versus beta-lactam antibiotics or tetracyclines. strains of b. fragilis were inhibited by 4--greater than 128 micrograms benzylpenicillin or cephalothin/ml, 1.0--64 micrograms cefoxitin/ml, 0.064--2 micrograms clindamycin or metronidazole/ml, 2--8 micrograms chloramphenicol/ml, 2--16 micrograms fusidic acid/ml and 0.0 ...197937593
anaerobic pulmonary infections.the main cause of anaerobic pulmonary infections is aspiration of saliva, upper airway secretions or gastric content. predisposing conditions include prominent dental disease, chronic upper respiratory tract infections and reduced consciousness. fusobacterium nucleatum, bacteroides melaninogenicus and anaerobic gram-positive cocci are the most frequently encountered organisms. the clinical presentations are lung abscess, lung gangrene and empyema, which all tend to take a slow and indolent cours ...197937594
the predominant cultivable microbiota of the periodontal abscess. 197938308
improved procedure for transport of dental plaque samples and other clinical specimens containing anaerobic bacteria.an improved transport system for samples containing anaerobic bacteria was developed. this system increased the recovery rate of anaerobic bacteria up to 28.8% as compared to a commonly used method.197939087
[anaerobic gram negative septicemias in digestive tract surgery and in surgical intensive care]. 197939472
the porphyrin pigmentation of subspecies of bacteroides melaninogenicus.various subspecies of bacteroides melaninogenicus differ in their pigmentation. subsp. asaccharolyticus produces protohaem almost exclusively, subsp. intermedicus both protohaem and a smaller proportion of protoporphyrin, and subsp. melaninogenicus mainly protoporphyrin with a trace of protohaem. as a consequence young colonies can be differentiated by their red fluorescence in u.v. light (365nm): subsp. asaccharolyticus does not fluoresce, subsp. intermedicus shows a limited fluorescence, and s ...197939545
characterization of anaerobic gram-negative bacilli by using rapid slide tests for beta-lactamase production.a total of 175 isolates of anaerobic gram-negative bacilli were tested for beta-lactamase production by using a slide test modification of the chromogenic cephalosporin (nitrocefin, glaxo, middlesex, england) assay and the iodometric slide test. included isolates were bacteroides melaninogenicus (46), b. fragilis (78), other bacteroides isolates (21), fusobacterium (25), and other gram-negative bacilli (5). both slide tests detected 25 b. melaninogenicus isolates that were beta-lactamase produce ...197941001
gas abscess transformation of a huge hypernephroma. 197941445
anaerobic isolates in chronic recurrent suppurative otitis media. treatment with carbenicillin alone and in combination with gentamicin.tympanocentesis was performed in 32 pediatric patients with chronic recurrent suppurative otitis media. the aspirate was cultured aerobically and anaerobically. aerobes were isolated from ten patients (31.2%); anaerobes from one patient; and both aerobes and anaerobes from 21 patients (65.6%). there were 46 aerobic isolates. the aerobes commonly recovered were pseudomonas aeruginosa (24 isolates) proteus sp. (5) and staphylococcus aureus (3). there were 32 anaerobes isolated including anaerobic ...197941811
fluorescent antibody test kit for rapid detection and identification of members of the bacteroides fragilis and bacteroides melaninogenicus groups in clinical specimens.the fluoretec fluorescent antibody test kit (pfizer inc., new york, n.y.), developed for the rapid detection of members of the bacteroides fragilis and b. melaninogenicus groups, was evaluated by testing 58 stock cultures and 76 clinical specimens. the test reagents detected 100% of 40 b. fragilis and b. thetaiotaomicron stock culture strains, although only 22% of 18 b. vulgatus, b. distasonis, and b. ovatus strains showed positive fluorescence. the 76 clinical specimens were evaluated by examin ...197941847
rapid diagnosis of anaerobic infections by gas-liquid chromatography.it was postulated that the short chain fatty acids (scfa) produced by anaerobic bacteria might serve as microbial markers in purulent material. eighteen pus specimens from various sources were analysed by gas-liquid chromatography (glc), and the scfa detected were compared with the microorganisms isolated by conventional methods. it was found that the detection of propionic, isobutyric, butyric, or isovaleric acids by direct glc of pus specimens is strong evidence for anaerobic infection but not ...197941850
clinical and microbiological aspects of chemotherapeutic agents used according to the specific plaque hypothesis.certain forms of dental decay and periodontal disease appear to be due to specific bacterial infections following overgrowth of certain indigenous plaque bacteria, i.e., the specific plaque hypothesis, if so, then antimicrobial treatment based on a diagnosis of elevated levels or proportions of these organisms should be considered. such treatment cannot be administered according to concepts of the non-specific plaque hypothesis. a treatment philosophy is presented which is based upon considerati ...197941862
survival of crevicular microorganisms in an artificial culture medium. 197943361
detection of anticapsular antibodies to bacteroides asaccharolyticus in serum from rabbits and humans by use of an enzyme-linked immunosorbent assay.a sensitive serologic test, the enzyme-linked immunosorbent assay (elisa), was used to detect serum igg antibodies directed specifically to a capsular antigen of bacteroides asaccharolyticus (previously known as bacteroides melaninogenicus subspecies asaccharolyticus). anticapsular igg was measured in 30 specimens of rabbit serum after the animals were immunized with whole b. asaccharolyticus, the two subspecies of b. melaninogenicus, and several other bacterial species. species-specific anticap ...197944309
comparative ultrastructure of bacteroides melaninogenicus subspecies. 197944319
isolation of anaerobes in ludwig angina. 197931420
sphingolipid biosynthesis and vitamin k metabolism in bacteroides melaninogenicus.b. melaninogenicus provides a unique system for the study of the biosynthesis of an important group of lipids, the phosphosphingolipids. sphingolipid biosynthesis can be repressed and induced by depletion and restoration of vitamin k. at least one enzyme involved in sphingolipid biosynthesis from the microorganism can be solubilized and so purified by conventional methods. pathways involved in biosynthesis may differ from hitherto postulated pathways, for example, the incorporation of nh4+ into ...197932765
black-pigmented bacteroides from clinically characterized periodontal sites. 197944523
immunochemical characterization of surface antigens of bacteroides melaninogenicus.major surface antigens of bactmbrane complex by gentle methods, purified, and characterized immunochemically. a lipopolysaccharide (lps) was found to be chemically distinct from the lps of facultative gram-negative bacteria in that it lacked two core sugars, 2-keto-3-deoxyoctonate and heptose, as well as beta-hydroxymyristic acid, the predominant fatty acid in the lipid a moiety. the lps was further atypical in that it had a very low level of biologic activity. a capsular polysaccharide was demo ...197944922
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...197944923
virulence factors of anaerobic bacteria: summary of the symposium. 197944924
genetic relationship between different subspecies of bacteroides melaninogenicus. 197945296
metronidazole in the prophylaxis and treatment of anaerobic infection.the influence of prophylactic metronidazole on vaginal carriage rates of anaerobes and the development of postoperative anaerobic infection was studied in 104 women who underwent abdominal hysterectomy. metronidazole prophylaxis in 54 patients led to a decrease in the anaerobe vaginal carriage rate from 65% pre-operatively to 17% and 28% on the 3rd and 7th postoperative days respectively. in the control group (50 patients) no significant decrease in anaerobe yield was noted, corresponding percen ...197833454
complement activation by propionibacterium acnes and bacteroides melaninogenicus. 197833641
acute suppurative parotitis caused by anaerobic bacteria: report of two cases.staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ...197832513
[are periodontal diseases specific infectious diseases?]. 197844349
the influence of environment on the oral flora of shangaan mineworkers. 197844502
a method for the isolation of bacteroides melaninogenicus from the human mouth.isolation procedures involving the direct plating of specimens and the use of selective, non-selective, transport and enrichment media were compared in respect of their value for the recovery of bacteroides melaninogenicus from the gingival sulcus. a selective medium containing kanamycin and vancomycin enhanced the recovery rate of b. melaninogenicus. vmgii transport medium was convenient to use and gave as good a recovery rate as that obtained with plates directly inoculated in the clinic.197826805
isolation and characterization of bacteroides melaninogenicus from human specimen (author's transl). 197827649
early diagnosis of crepitant gangrene caused by bacteroides melaninogenicus.a rapid non-invasive test for the presence of b. melaninogenicus in the wounds of crepitant non-clostridial gangrene is described. the wounds are viewed under an ultraviolet light, and the presence of bright red fluorescene indicates the probable presence of b. melaninogenicus.197827832
beta-lactamases in bacteroides.bacteroides fragilis is responsible for most anaerobic infections in man. most isolates of b. fragilis show resistance to beta-lactam antibiotics. this resistance might be due to beta-lactamase production or permeability barrier in the cell wall. b. fragilis produce beta-lactamase with mainly cephalosporinase activity. other bacteroides species such as b. clostridiformis, b. melaninogenicus and b. oralis also produce beta-lactamase but with different biochemical characteristics.197828561
metronidazole in surgical infections. 197829029
pleuropulmonary lung infection by anaerobic bacteria.two patients with severe pleuropulmonary infection caused by non-sporing anaerobic bacteria are described. one had an empyema with foul-smelling pus and developed bacteraemia, and the other developed a lung abscess. both were successfully treated with antibiotics and drainage, chest surgery being avoided. the successful diagnosis and treatment of these patients involved close liaison between clinical and laboratory staff.197829654
preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria.several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. these include use of a check valve for the production of prereduced anaerobically sterilized media; a salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobica ...197829909
role of nucleosides, 5-phosphoribosyl-1-pyrophosphate, and ribose 1-phosphate in the biosynthesis of phosphosphingolipids in bacteroides melaninogenicus. 197823730
attachment of bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets.this investigation examined the ability of cells of bacteroides melaninogenicus subsp. asaccharolyticus 381 to adhere to surfaces that might be important for its initial colonization of the mouth and its subsequent colonization in periodontal pockets. of 48 asaccharolytic strains of b. melaninogenicus, 47 agglutinated human erythrocytes, whereas none of 20 fermentative strains, which included reference cultures of the subspecies intermedius and melaninogenicus, were active. electron microscopy i ...197824002
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...197824059
immunochemical and biologic studies of the lipopolysaccharide of bacteroides melaninogenicus subspecies asaccharolyticus. 197824073
the nature of clotting and fibrinolytic activities of bacteroids melaninogenicus.from the supernatant of b. melaninogenicus ss. asaccharolyticus culture, a protein fraction was isolated by ethanol precipitation. the fraction was tested for the presence of clotting and fibrinolytic activities by application of quantitative techniques and specific substrates for measurement of prothrombin and plasminogen activation, and collagenase and elastase activity. it is postulated that ability of bacteroides melaninogenicus ss. asaccharolyticus extracellular factors to clot fibrinogen a ...197824965
a rapid slide test for penicillinase.the authors developed a rapid slide test modification of the iodometric method for detection of penicillinase produced by organisms growing on routine plating media. a loopful of colonies is scraped from the agar surface and emulsified in one drop of an iodine-penicillin solution on a glass slide. addition of a drop of 0.4% starch solution results in a purple color when penicillinase is not present; a colorless reaction denotes a positive test. the slide test yielded positive results identical t ...1978345799
performance of converted pressure cookers and two conventional jars for anaerobic bacterial culture.the simple conversion of commercial pressure cookers into inexpensive anaerobic jars is described. these containers were shown to be as good as the small conventional bbl polycarbonate gaspak and large vented 150 gas-replacement jars when assessed by means of three biological indicators: pseudomonas aeruginosa, bacteroides melaninogenicus, and bacteroides fragilis. ps. aeruginosa seeded on simmond's citrate agar was shown to be the most sensitive indicator of the three for traces of oxygen.1978348726
microflora of the cervix during normal labor and the puerperium.the normal flora of the cervix was studied in 50 healthy women in labor and on the third day postpartum. aerobic bacteria were the exclusvie isolates in 25% of these women, and 75% had anaerobic bacteria in addition to aerobes. anaerobes were always found in association with aerobes. the most frequent isolate of bacteroides was bacteroides melaninogenicus, found in 20% of the women during labor and 14% during the puerperium. bacteroides fragilis was distinctly absent during labor but was found i ...1978351071
bacteriological examination of summermastitis secretions. the demonstration of bacteroidaceae.corynebacterium pyogenes was isolated from 22, peptococcus indolicus from 22, a microaerophilic coccus from 21, bacteroides melaninogenicus subsp. melaninogenicus from 18, fusobacterium necrophorum from 15, and various unidentified anaerobes from 9 of a total of 22 summermastitis secretions examined.1978351551
the bacteriology of cavitating pulmonary infections and empysema. studies of transtracheal aspirates and pleural fluid.a prospective study of bacteriology in 14 cases of cavitating pulmonary infections and empyema is reported. bacteriologic results were based on transtracheal aspirates or pleural fluid. appropriate anaerobic bacteriologic methods were employed. anaerobic bacteria were recovered in 11 patients (79%); they were the only pathogens isolated in 6 patients. the predominant species were fusobacterium nucleatum, bacteroides melaninogenicus, bacteroides fragilis and peptostreptococcus. aerobic bacteria w ...1978352421
immunological study of the pili of bacteroides melaninogenicus. 1978292529
[capsular-like structure in b. melaninogenicus as revealed by gins' negative staining method (author's transl)]. 197893958
growth inhibition of streptococcus mutans by the black pigment (haematin) of bacteroides melaninogenicus. 197831156
severe arm pain associated with pathological bone cavity of maxilla. 197874557
abscesses of the frontal lobe of the brain secondary to covert dental sepsis.the bacterial species found in pus aspirated from brain abscesses in two patients were typical of those found in dental sepsis. subsequently apical-root abscesses were demonstrated in the upper jaws of both patients. this evidence strongly suggests that these cerebral abscesses were secondary to dental sepsis which could have spread from the teeth to the frontal lobes by several possible antaomical pathways.197879867
chemotherapy of brain abscess. 197882092
identification of a subspecies-specific capsular antigen from bacteroides melaninogenicus subspecies asaccharolyticus by immunofluorescence and electron microscopy.an indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of bacteroides melaninogenicus subspecies asaccharolyticus. all of 23 strains of b. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this test. all 11 strains of bacteroides melaninogenicus subspecies intermedius tested and three strains of bacteroides melaninogenicus subspecies melaninogenicus were fluorescence-negative. thirty-one strai ...197884036
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
anaerobic and aerobic urethral flora in healthy females.we characterized the aerobic and anaerobic urethral flora of five healthy females by performing urethral and midstream urine cultures once weekly for 8 weeks. aerobic cultures were performed monthly for an additional 3 months. lactobacillus spp. were isolated from 52 of 57 samples, staphylococcus epidermidis from 42 of 57, corynebacterium spp. from 26 of 57, and alpha-hemolytic streptococci from 14 of 57. two subjects had e. coli serogroup o6 and group b streptococci isolated on five occasions, ...1978566769
[finding of anaerobic bacteria in blood cultures].out of the blood cultures sent to the bacteriology laboratory of hospital del niño dif, during the period of one year, in 1.4% of samples, isolation of anaerobic bacteria was obtained. isolation was more frequent in infants under one year of age, but specially in newborns. the species most frequently found were propionibacterium acnes, bacteroides melaninogenicus and clostridium subterminale. the presence of this finding in blood cultures of infants is noted, but there is no purpose to establish ...1978619923
enhancement of experimental anaerobic infections by blood, hemoglobin, and hemostatic agents.certain foreign materials have been demonstrated to enhance the infectivity of aerobic and anaerobic bacteria. whole blood and other protein compounds encountered in surgical settings or trauma were tested for their effect on infectivity of nonsporeforming anaerobic bacteria. infectious synergistic mixtures of bacteroides fragilis plus peptostreptococcus anaerobius and bacteroides melaninogenicus plus fusobacterium necrophorum were each diluted to a barely noninfectious or minimally infectious c ...1978631883
anaerobic microflora of the vagina in children.vaginal cultures from 25 healthy girls from two months to 15 years of age were examined for aerobic, facultatively anaerobic, and obligately anaerobic bacteria. an average of 8.7 species (3.7 aerobic and facultatively anaerobic species; 5.3 obligately anaerobic species) were isolated from these cultures. staphylococcus epidermidis (21), diphtheroids (20), bacteroides (19), peptococci (19), peptostreptococci (14), and bacteroides melaninogenicus (14) were most prevalent organisms.1978686083
dihydrosphingosine growth inhibition and repression of 3-ketodihydrosphingosine synthetase activity in bacteroides melaninogenicus. 1978697822
susceptibility of anaerobic bacteria to ten antimicrobial agents.the susceptibility pattern of 265 anaerobic bacteria from clinical isolates to 10 antimicrobial agents was investigated by the agar dilution technique. penicillin g, in a concentration of 16 mug/ml, was active against most organisms, important exceptions being 12% of bacteroides melaninogenicus and 24% of b. fragilis strains. the susceptibility of strains to ampicillin was similar to their susceptibility to penicillin g. carbenicillin, at </=128 mug/ml, inhibited all but a few strains. cefamando ...1978708014
bacteriology of human experimental gingivitis: effect of plaque and gingivitis score.the plaque flora isolated from discrete dentogingival sites during a human gingivitis experiment was analyzed as a function of the plaque score and of the gingivitis score. when the gingivitis score was plotted as a function of the plaque score, a nonbleeding gingivitis was associated with a proportional increase in the actinomyces sp. at the expense of the streptococcus sp. in particular, the percentage of actinomyces israelii increased significantly, while the percent streptococcus sanguis dec ...1978711337
inhibition of phagocytosis in vitro by obligate anaerobes.a variety of species of obligate anaerobes have been shown to interfere with the phagocytosis and killing of proteus mirabilis and other aerobic bacteria in vitro. although all the obligate anaerobes examined showed this activity, the effect was greatest with strains of bacteroides melaninogenicus and b. fragilis. in contrast, none of the 36 aerobes tested acted in this way. these observations suggest that the presence of obligate anaerobes may be fundamental to the pathogenesis of some types of ...197773952
the predominant cultivable microflora of advanced periodontitis.a study on the predominant cultivable microorganisms harbored in the base of deep periodontal pockets of eight patients aged 34-48 years was carried out using the roll tube culture technique. from a total of 475 isolates, 425 (89.5%) were obligate anaerobes, 356(74.9%) were gram-negative, and 441(92.8%) were categorized as rods. bacteroides melaninogenicus and fusobacterium nucleatum constituted the majority of the isolates in seven samples, but their mutual proportions differed considerably bet ...1977320648
clinical evaluation of a simple, rapid procedure for the presumptive identification of anaerobic bacteria.a simple, rapid procedure for the presumptive identification of anaerobic bacteria has been evaluated. two hundred and thirty-five clinical isolates were identified using gas-liquid chromatography and 3-ml volumes of a few selected test media. these test media were stored aerobically and incubated in gaspak anaerobic jars. the average incubation time was 39 h. this procedure, when compared to the results of our standard identification procedure, correctly identified 98% of the isolates to the ge ...1977323283
lethality for mice and chick embryos, pyrogenicity in rabbits and ability to gelate lysate from amoebocytes of limulus polyphemus by lipopolysaccharides from bacteroides, fusobacterium and veillonella.phenol-water extracted lipopolysaccharides (lps) from veillonella, fusobacterium nucleatum, bacteroides fragilis and bacteroides melaninogenicus were lethal for mice and 11-days-old chick embryos, pyrogenic in rabbits, and gelated limulus amoebocyte lysate. mouse lethality was considerably enhanced by actinomycin-d. in all test systems the endotoxin activity of veillonella and fusocbacterium lps was comparable to that of lps from salmonella enteritidis, which was included as a reference endotoxi ...1977564596
[in vitro susceptibility of bacteroidaceae to cefoxitin and cephalothin (author's transl)].the activity of the new cephamycin c derivatives cefoxitin against 102 clinical isolates of bacteroidaceae (95 bacteroides strains and 7 fusobacterium-sphaerophorus cultures) was determined by tube dilution and standardized agar diffusion tests and compared to that of cephalothin. cefoxitin was more active than cephalothin against 56 bacteroides fragilis strains, 19 bacteroides thetaiota-omicron strains and two bacteroides splanchnicus strains. the difference seemed less pronounced with five bac ...1977856727
bacteriocin-like activities of human dental plaque flora against oral anaerobic microorganisms. 197726477
clotting and fibrinolytic activities of bacteroides melaninogenicus.106 strains of bacteroides melaninogenicus ss. asaccharolyticus, intermedius and melaninogenicus were tested for production of extracellular blood clotting and fibrinolytic factors. 78.3% of tested strains caused clotting of rabbit plasma and 79.2% activated human plasminogen. strains producing the clotting or fibrinolytic factor only were isolated. both factors were quite active, as positive results for most strains were detected within one hour of incubation in controlled test system.197724963
the hydrolysis of dextran by gram negative non-sporing anaerobic bacilli. 197724035
beta-lactamase activity in strains of bacteroides melaninogenicus and bacteroides oralis.beta-lactamase from strains of bacteroides melaninogenicus and bacteroides oralis hydrolyzed penicillin more rapidly than ampicillin or carbenicillin. cephalothin and a chromogenic cephalosporin (87/312) were also hydrolyzed by the enzyme. activity was found only in beta-lactam-resistant strains, but there was considerable variation in activity among strains having the same minimal inhibitory concentrations of antibiotic. beta-lactamase activity was cell bound and appeared to be tightly associat ...197713705
fermentation of l-aspartate by a saccharolytic strain of bacteroides melaninogenicus.resting cells of bacteroides melaninogenicus fermented l-14caspartate as a single substrate. the 14c-labeled products included succinate, acetate, co2, oxaloacetate, formate, malate, glycine, alanine, and fumarate in the relative percentages 68, 15, 9.9, 2.7, 1.8, 1.0, 0.7, 0.5, and 0.06, respectively, based on the total counts per minute of the l-14caspartate fermented. ammonia was produced in high amounts, indicating that 96% of the l-aspartate fermented was deaminated. these data suggest that ...197713713
an unusual bilateral pathological fracture.an unusual case of a bilateral pathological fracture of the mandible has been presented. the fracture of the right side of the mandible was through an area of bacteroides osteomyelitis and the fracture of the left side of the mandible was through a large cementifying fibroma, which had replaced the left body of the mandible.197711280
casamino acids enhance growth of bacteroides melaninogenicus.casamino acids enhance the growth of bacteroides melaninogenicus when added to various concentrations of trypticase. absence of a peptide, not amino acids, is responsible for the inability of casamino acids to support growth.197712141
leukocyte migration inhibition by bacterial antigens in patients with periodontal disease. 197712257
treatment of bacteroides endocarditis with carbenicillin.a heroin addict developed acute bacterial endocarditis with streptococcus viridans and bacteroides melaninogenicus. although blood cultures became negative during penicillin g and clindamycin therapy there was little clinical response. prompt clinical improvement was achieved with intravenous carbenicillin in a dose of 40 g daily. in vitro testing supported the superiority of carbenicillin therapy in this patient.197715455
the syndrome of inappropriate secretion of antidiuretic hormone associated with anaerobic thoracic empyema.the syndrome of inappropriate secretion of antidiuretic hormone has been associated with many pulmonary diseases, including tuberculosis and bacterial and viral pneumonia: however, it has not been reported with anaerobic infections or empyema in the absence of pneumonia. we report a patient with empyema due to bacteroides melaninogenicus, bacteroides oralis, and peptostreptococcus who developed the syndrome. eight hours before the start of therapy, his serum sodium concentration was 127 meq per ...197715491
energy-dependent incorporation of sphingolipid precursors and fatty acids in bacteriodes melaninogenicus.washed cells of bacteroides melaninogenicus are unable to incorporate the sphingolipid precursor 3-ketodihydrosphingosine (3kds) or dihydrosphingosine into the complete sphingolipids ceramide phosphorylethanolamine (cpe) and ceramide phosphorylglycerol (cpg), whereas growing cultures are able to do so. this result suggested that an energy source was required by washed cells to initiate the incorporation of 3kds. investigation of a number of energy sources for b. melaninogenicus showed that gluta ...197715984
purification and immunochemical characterization of the outer membrane complex of bacteroides melaninogenicus subspecies asaccharolyticus.morphologic study of bacteroides melaninogenicus subspecies asaccharolyticus by electron microscopy disclosed the presence of a capsule and a cell wall structure otherwise typical of a gram-negative organism. an outer membrane complex was isolated with use of gentle methods. relative purity of the preparation was confirmed by electron microscopy and by the formation of a single band in a sucrose density gradient. gel chromatography was used for separation of the major components of the membrane. ...197716065
penicillin resistance and penicillinase production in clinical isolates of bacteroides melaninogenicus.the minimum inhibitory concentrations (mic) of penicillin and six other antimicrobials were determined for 50 clinical isolates of bacteroides melaninogenicus. agar dilution susceptibilities were performed using supplemented brucella blood agar and the proposed national committee for clinical laboratory standards standard method for anaerobes; results with the two methods were comparable. a penicillin concentration >/=0.8 mug/ml was needed to inhibit 56% of the isolates, whereas 100% were suscep ...197716564
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