Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the role of blastocystis hominis as a possible intestinal pathogen in travellers. | the role of blastocystis hominis as a pathogen for man has been controversially discussed, while travel history has been implicated as a risk factor of infection. few controlled studies of the association between b. hominis and symptomatic diseases have been performed. therefore, a case-control study among 795 german tourists returning from tropical countries was conducted. the prevalence of the organism among patients with and without symptoms was assessed. blastocystis hominis was detected in ... | 1997 | 9279726 |
| survival of blastocystis hominis clones after exposure to a cytotoxic monoclonal antibody. | our previous studies have shown that monoclonal antibodies (mabs) to blastocystis hominis react mainly with carbohydrate epitopes, while 1 mab (1d5) reacts specifically with a protein of 30.5 kda. in the present study, 3 monoclonal antibodies (1d5, 1e7 and 4f7) were used in immunogold localization. 1e7 and 4f7 were found to react primarily with the surface coat, while 1d5 was plasma membrane-specific. in the presence of complement, only 1d5 exhibited a cytotoxic effect on b. hominis whereas 1e7 ... | 1997 | 9292311 |
| [clinical significance of blastocystis hominis infection: epidemiologic study]. | to evaluate the frequency of blastocystis hominis parasitation and to ascertain its role as an intestinal a prospective study during 18 months pathogen has been carried out. | 1997 | 9303956 |
| parasites elicited cross-reacting antibodies to opisthorchis viverrini. | two batches of crude antigens extracted from adult opisthorchis viverrini worms were compared. one was derived from adult worms harvested from the livers of laboratory infected hamsters and another was obtained from worms sedimented from the faeces of opisthorchiasis patients following treatment with praziquantel. sds-page and coomassie brilliant blue staining revealed that the two preparations had similar protein components of which the predominant ones were the 17-18 kda doublet. the antigens ... | 1997 | 9346276 |
| blastocystis hominis among apparently healthy food handlers in jeddah, saudi arabia. | a prospective study was carried out to investigate the prevalence of blastocystis hominis among random sample of apparently healthy food handlers. a total of 250 non saudi males over 21 years of age were examined. ninety (36%) had pathogenic and non pathogenic intestinal parasites. a total of 143 parasites were detected in their stool specimens. twenty were b. hominis (13.99%) while other parasites were 123 (86.01%). b. hominis was found in 20 positive cases (22.22%) with an overall rate of 8%. ... | 1997 | 9425825 |
| usefulness of new dna extraction procedure for pcr technique in species identification of entamoeba isolates. | in this study we have developed a modified clark and diamond (1992, 1993) method using the polymerase chain reaction to amplify amoebic ribosomal rna genes that allow either specific detection of entamoeba histolytica s. str. or amoeba species identification. dna was isolated from cultured protozoa or from stool samples (cysts and/or trophozoites). cultures of xenic or axenic material (also stool samples) were treated with easy genomic dna prep or genomic dna prep plus (a&a biotechnology, poland ... | 1997 | 9374591 |
| [parasitosis in an adult population with chronic gastrointestinal disorders]. | we worked with 185 middle-class patients above 18 years of age, both sexes, who presented diarrhea and/or chronic gastrointestinal disorders. the faeces were collected serially in formol 10% and processed in the following way: direct microscopy, with and without wet staining, concentration by ritchie's method, 1% safranine technique for a specific investigation of cryptosporidium sp., and faecal sieving macroparasites. twenty eight point six of the studied patients showed at least one enteropara ... | 1997 | 9412130 |
| nitazoxanide for the treatment of intestinal protozoan and helminthic infections in mexico. | a study to evaluate the efficacy and safety of nitazoxanide as a single agent for the treatment of a broad spectrum of mixed parasitic infections, both protozoa and helminths, was conducted at a primary school in san pedro tolimán, querétaro, mexico. three faecal samples from 1824 adults and children were screened for the presence of oocysts, cysts, trophozoites, eggs or larvae of intestinal protozoa or helminths. two hundred and forty-six adults and children infected with at least one protozoan ... | 1997 | 9580117 |
| [extraintestinal blastocystosis. a review of the literature]. | 1997 | 9380947 | |
| isoenzyme patterns of blastocystis hominis patient isolates derived from symptomatic and healthy carriers. | isolates of blastocystis hominis derived from patients with intestinal symptoms and from healthy carriers were cultured in vitro and isoenzyme patterns of hexokinase (e.c. 2.7.1.1), phosphoglucomutase (e.c. 2.7.5.1) and glucose phosphate isomerase (e.c. 5.3.1.9) were investigated to find evidence for pathogenic and non-pathogenic subspecies of b. hominis. for this purpose we examined 2000 patients of the out-patient department of the institute for tropical medicine in tübingen. from these, we ob ... | 1997 | 9491103 |
| [human infection by intestinal protozoa and helminths in calbuco county, x region, chile, 1997]. | by the performance of parasitological examination of one fecal sample per individual, a total of 256 persons from a rural county in the x region (41 degrees 50 minutes south lat., 73 degrees 05 minutes west long.) were studied. the general rates of infection by intestinal parasite and/or commensal protozoa and helminths found were: giardia intestinalis 14.1%, entamoeba histolytica 11.7%, blastocystis hominis 36.0%, entamoeba coli 9.8%, endolimax nana 16.4%, iodamoeba buetschlii 1.2%, chilomastix ... | 1997 | 9497539 |
| variation in the cyst morphology of blastocystis hominis. | two types of blastocystis hominis cyst are passed in human feces, one with an outer fibrillar coat and the other without it. the outer fibrillar coat is shed as the cyst matures, but another fibrillar coat forms that is closely attached to the cyst wall and is clearly visible at high magnification in the electron microscope. the internal structure of the two cyst form is similar. | 1997 | 9089732 |
| significantly increased igg2 subclass antibody levels to blastocystis hominis in patients with irritable bowel syndrome. | blastocystis hominis is a common intestinal parasite of humans in the tropics whose pathogenic role is in dispute. its presence has been reported in a variety of intestinal disorders resembling irritable bowel syndrome (ibs) such as diarrhea, anorexia, and flatulence. we have therefore investigated a possible link between ibs and blastocystosis by determining igg antibody levels to b. hominis in patients with ibs. levels of igg antibodies were significantly elevated in patients with ibs compared ... | 1997 | 9129532 |
| blastocystis hominis as a potential cause of diarrhea in aids patients: a report of six cases in bahia, brazil. | the role of blastocystis hominis as a human pathogen is controversial, although there is some evidence suggesting that it is the agent implicated in causing diarrhea in immunocompromised patients. we report 6 cases of aids patients presenting with diarrhea with no agents identified in their stools exceptb. hominis. in all cases, treatment was followed by complete recovery from symptoms and clearance of b. hominis from the patients' stools. in 2 cases, relapse of diarrhea was followed by a positi ... | 1997 | 11107245 |
| [external quality assessment in coproparasitology in havana city province, cuba] | an external quality assessment in coproparasitology was carried out in 77 laboratories from havana city. a questionnaire and ten plastic vials with different intestinal parasites in a small nylon bag, duly sealed, were sent to each laboratory. answers were collected during the 72 hours after delivery. results were analyzed by means of a computer program. the majority of the laboratories (70%) passed the test; the municipalities with the worst scores in the province were lisa, marianao, and haban ... | 1997 | 10886830 |
| [intestinal parasitosis: our series from 1992 to 1995]. | from 1992 to 1995 in our department 2236 researches of intestinal parasites (2194 on stool specimens, 34 on scotch tests, 8 on enterotests) were carried out on 1200 patients (703 hiv-ab negative and 497 hiv-ab positive). on the whole 387 samples (17.34%) of 203 subjects (16.92%) were found parasitized; 92 (13.08%) patients of them were hiv-ab negative and 111 (22.33%) were hiv-ab positive. we found more frequently blastocystis hominis and giardia intestinalis among hiv-ab negative subjects and c ... | 1996 | 12858028 |
| prevalence of enteropathogens in stools of rural maasai children under five years of age in the maasailand region of the kenyan rift valley. | stool samples were collected during august 1994 from seventy rural maasai children under the age of five years who were living in the maasailand region of the kenyan rift valley. microbiological analysis was carried out on these samples to identify which intestinal pathogens were present among the infant population of the maasai. of the samples studied 54% were pathogen positive. the most common pathogen isolated was giardia lamblia which was detected in 31% of the samples. other pathogens that ... | 1996 | 8625866 |
| [blastocystis hominis in a zone of northern italy]. | 1996 | 8650434 | |
| blastocystosis or zierdt-garavelli disease. | 1996 | 8692759 | |
| dna polymorphism revealed by arbitrary primers polymerase chain reaction among blastocystis strains isolated from humans, a chicken, and a reptile. | dna polymorphisms of different strains of blastocystis isolated from humans, a chicken, and a reptile were examined by an arbitrary primer pcr method. two strains of blastocystis hominis isolated from humans in the usa and japan yielded nearly identical pcr products. however, one strain of b. hominis (isolated from a human in singapore) yielded quite different pcr products. blastocystis sp. isolated from a chicken yielded pcr products similar to those of the former two strains, while blastocysti ... | 1996 | 8720942 |
| hemorrhagic proctosigmoiditis and blastocystis hominis infection. | 1996 | 8534017 | |
| freeze-fracture cytochemistry of membrane cholesterol in blastocystis hominis. | membrane cholesterol in blastocystis hominis was detected by freeze-fracture methods using a polyene antibiotic, filipin. since the intramembrane particles (imp) were distributed heterogeneously on both plasma and vacuole membranes, many imp-free areas were observed. even in filipin-treated cells, filipin-cholesterol complexes were not detected in imp-free areas on the plasma membrane, whereas on the central vacuole membrane the complexes were mainly observed in imp-free regions. these results i ... | 1996 | 8982792 |
| [chronic diarrheic syndrome from blastocystis hominis]. | 1996 | 8983372 | |
| the occurrence of blastocystis hominis in hiv-infected patients. | 1996 | 8728052 | |
| observations on the ultrastructure and viability of the cystic stage of blastocystis hominis from human feces. | this report describes the ultrastructure and viability of cysts of blastocystis hominis from feces of infected patients. the cysts were round to ovoid, measured 2-5 microns in size, and contained a condensed cytoplasm that had vacuoles of varying sizes, four nuclei, and as many as six cristate mitochondria. the cell wall was rather electron-lucent. surprisingly, chromatoid-like structures were found in the cytoplasm and nucleus of some of the cysts. these have not previously been reported in bla ... | 1996 | 8738284 |
| [protozoan infection (blastocystis hominis) concomitant with pseudomonas sp. peritonitis in continuous ambulatory peritoneal dialysis (capd)]. | case-report of protozoal infection (blastocystis bominis) during pseudomonas peritonitis in male patient with intestinal diverticulosis on continuous ambulatory peritoneal dialysis (capd) treatment for chronic renal failure (crf). microscopic morphology and cultural characteristics are summarized from current literature. photographic images in phase contrast from fresh-observation of faeces and peritoneal fluid are reported. although other protozoa (e.g. acanthamoeba free-living) have already be ... | 1996 | 8848771 |
| blastocystis hominis revisited. | blastocystis hominis is a unicellular organism found commonly in the intestinal tract of humans and many other animals. very little is known of the basic biology of the organism, and controversy surrounds its taxonomy and pathogenicity. there morphological forms (vacuolar, granular, and ameboid) have been recognized, but recent studies have revealed several additional forms (cyst, avacuolar, and multivacuolar). the biochemistry of the organism has not been studied to any extent, and organelles a ... | 1996 | 8894352 |
| clonal growth of blastocystis hominis in soft agar with sodium thioglycollate. | the present report describes a method for establishment of colonies of blastocystis hominis from single cells in soft agar. the percentage of colony-forming efficiency (% cfe = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. five human blastocystis isolates chosen for this study showed no apparent variation in colonial morphology. isolated colonies were also successfully grown in liquid medium, providing a ... | 1996 | 8897510 |
| intestinal parasites in children and adults with and without abdominal discomfort from the kathmandu area of nepal. | the present study on intestinal parasites from the kathmandu area of nepal found that in subjectively healthy children (hc) and adults (ha) the total parasite load was 28.1% and 38.8%, respectively, whereas children (adc) and adults (ada) with abdominal discomfort had a load of 62.7% and 67.8%. the prevalence of nematodes in the 4 groups was significantly higher in those with abdominal discomfort, particularly of hookworm, enterobius, and ascaris. hymenolepis nana was the most common tapeworm, a ... | 1996 | 8783972 |
| phylogenetic position of blastocystis hominis that contains cytochrome-free mitochondria, inferred from the protein phylogeny of elongation factor 1 alpha. | 1996 | 8813670 | |
| hemorrhagic proctosigmoiditis and blastocystis hominis. | 1996 | 8929001 | |
| human parasite finds taxonomic home. | 1996 | 8602239 | |
| obligate anaerobe or not. | 1996 | 8610009 | |
| blastocystis hominis infection. | 1996 | 8684227 | |
| [blastocystis hominis infection: a case report]. | blastocytis hominis has long been described as a non-pathogenetic protozoan parasite until recently, when claims have been made that it could be the cause of intestinal disorders. the clinical picture of b. hominis consists of non specific abdominal pain, watery diarrhea, anorexia, vomiting and weight loss. rarely a more invasive form of the disease with rectal bleeding can occur. we describe the case of a ten year-old girl who was admitted to our hospital for diarrhea, abdominal pain and fever. ... | 1996 | 9091775 |
| colony formation of blastocystis hominis in soft agar. | this is the first description of a method for growing axenized blastocystis hominis as colonies in petri dishes containing soft agar. blastocystis cells cultured in two types of agar appeared to show different colonial morphologies as well as differing colony yields. microscopic examination of the colonies revealed many amoeboid and giant cells. many cells were also shown to possess thin filament-like structures that appeared to stretch across the central vacuole. | 1996 | 8740557 |
| gastrointestinal parasite infections. | 1996 | 8843840 | |
| intestinal parasitism in san cayetano, corrientes, argentina. | an epidemiologic study was conducted in san cayetano, a village in the province of corrientes, argentina, to determine the prevalence of intestinal parasitoses in children. eighty-eight households were randomly selected. stool samples were collected from 207 children (72% of the school-age population and 12% of the total village population) over a period of six consecutive days, and were subjected to microscopic examination. of the samples examined, 170 (83%) contained one or more parasites, of ... | 1996 | 8897723 |
| [case report of colitis associated with blastocystis hominis infection]. | 1996 | 8905973 | |
| resistance of blastocystis hominis cysts to metronidazole. | blastocystis hominis cysts concentrated from faeces were resistant to metronidazole up to a concentration of 5 mg/ml. a cysticidal drug is therefore needed for radical treatment, to avoid relapse and to prevent transmission. | 1996 | 8911453 |
| the diagnosis of blastocystis hominis cysts in human faeces. | blastocystis hominis cysts in faeces can be concentrated on ficoll column. they are pleomorphic in shape having a mean diameter of 6.65 microns (s.d. = 2.32). they may or may not have a membranous layer surrounding them. the membranous layer tends to come off with pressure or dying of the specimen and corresponds to the fibrillar layer observed under the electron microscope. the ficoll concentration technique is also useful for concentrating other protozoan cysts. | 1996 | 8842989 |
| severe blastocystis hominis in an elderly man. | we describe a unique case of severe blastocystis hominis infection in an elderly man with severe dehydration, marked leukocytosis and hypoalbuminaemia after antibiotic treatment for right pneumonia. the patient recovered after treatment with metronidazole. this case presentation demonstrates the ability of b. hominis to induce severe gastrointestinal manifestations and general deterioration, particularly in light of the controversy surrounding its possible potential pathogenicity. we believe, th ... | 1996 | 8842998 |
| production and characterization of murine monoclonal antibodies to blastocystis hominis. | several hybridomas producing antibodies detected by enzyme-linked immunosorbent assay (elisa) were established by fusions of mouse myeloma p3.x63.ag8.u1 with spleen cells from balb/c mice immunized against an isolate of blastocystis hominis. five strongly positive hybrids (6b6, 1d5, 1e7, 4f7 and 4g11) were cloned and all were found to secrete igm monoclonal antibodies. four mabs (6b6, 1e7, 4f7 and 4g11) reacted in immunoblots with a number of b. hominis antigens (mol. wt ranging from 25,000 to 2 ... | 1996 | 8773525 |
| identification of surface coat carbohydrates in blastocystis hominis by lectin probes. | the carbohydrate residues of the surface coat of 20 axenic cultures of blastocystis hominis were studied using fitc-labelled lectins (cona, wga, dba, hpa, sba, pna, ueai and lpa). the specific affinity of reactive lectins was determinated by competitive inhibition assay with specific carbohydrates or by enzymatic pre-treatment of cells. all stocks strongly bound cona and hpa; wga, ueai and lpa were partially reactive, and the remaining lectins were nonreactive. inhibition assays showed abolition ... | 1996 | 8818733 |
| axenic culture of reptilian blastocystis isolates in monophasic medium and speciation by karyotypic typing. | the growth of axenic reptilian isolates of blastocystis in iscove's modified dulbecco's medium (imdm) was studied and the morphology of the parasite was examined by phase-contrast microscopy. the chromosomal patterns of these reptilian isolates of blastocystis were examined by pulsed-field gel electrophoresis (pfge) and compared with those of b. hominis and b. lapemi, a sea snake blastocystis. imdm with 10% horse serum supported excellent growth of the reptilian blastocystis isolates. the parasi ... | 1996 | 8825212 |
| resistance of blastocystis hominis cysts to chlorine. | 1996 | 8936976 | |
| intestinal sarcocystosis in thai laborers. | to determine the prevalence of sarcocystis and other intestinal parasites in thai laborers who were going abroad for work, stool examinations of 362 asymptomatic laborers were studied. the four most frequently parasites found in stool were sarcocystis sp (23.2%), opisthorchis viverini (40.3%), hookworm (21.5%), and strongyloides stercoralis (14.1%). giardia intestinalis (5.2%), entamoeba coli (1.7%), endolimax nana (2.5%), blastocystis hominis (4.1%), echinostoma sp (3.6%), trichuris trichiura ( ... | 1996 | 9031398 |
| [identification of blastocystis hominis in concentrated fecal samples]. | 1996 | 9044649 | |
| in vitro response of blastocystis hominis against traditional chinese medicine. | this is the first inn vitro study on the activity of 20 kinds of crude extracts of traditional chinese medicine (tcm) on the intestinal parasite, blastocystis hominis using the criteria of living cell count (lcc) and living cell rate (lcr). lcc and lcr were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. lcr calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cel ... | 1996 | 9121165 |
| intestinal parasitism--protozoa and helminths--in primates at the barcelona zoo. | the faunistic results regarding intestinal parasitism by protozoa and helminths in 21 primate species (three cebidae, thirteen cercopithecidae, one hylobatidae, one lemuridae, three pongidae) are reported. the primate species were housed in four separate galleries. six faecal samples of each host species were subjected to coprological analysis. fifteen parasite species were detected: 11 protozoa (entamoeba coli, e. chattoni, e. hartmanni, iodamoeba bütschlii, endolimax nana, giardia intestinalis ... | 1996 | 9210027 |
| blastocystis hominis in human immunodeficiency virus-related diarrhea. | a wide variety of bacterial, viral, and parasitic pathogens can cause severe diarrhea in patients with advanced human immunodeficiency virus (hiv) infection. conflicting evidence exists as to whether blastocystis hominis should also be included among the infectious agents capable of causing hiv-related diarrhea. | 1995 | 8578192 |
| [blastocystis hominis: a common commensal in the colon. study of prevalence in different populations of paris]. | to compare the prevalence of blastocystis hominis in different population categories in paris, including immunodepressed subjects. | 1995 | 8545396 |
| practice guidelines for ordering stool ova and parasite testing in a pediatric population. the alberta children's hospital. | a comprehensive utilization review was done of all stool ova parasite examinations performed at a pediatric hospital during a 3-year period from june 1, 1989 to july 1, 1992. a total of 2,652 stool specimens were surveyed from 1,532 children. forty-one percent (1,081) of the workload was from inpatients, 37% (976) was from emergency room (er) visits/other outpatients, and 22% (233) was from patients attending the gastroenterology (gi) clinic. the prevalence of enteric parasites in hospitalized c ... | 1995 | 7677114 |
| biochemical characterisation of human isolates of blastocystis hominis. | sds-page and iso-enzyme analysis of 11 human isolates of blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. this is the first iso-enzyme and the second protein analysis to indicate strain differences in b. hominis. | 1995 | 7707340 |
| tubulovesicular elements in blastocystis hominis from the caecum of experimentally-infected rats. | by transmission electron microscopy (tem), tubulovesicular elements were seen in blastocystis hominis obtained from the caecum of experimentally-infected rats. these appeared to arise from the peripheral cytoplasm and were rounded, oval or elongate in sections. it is suggested that these elements form a network for transfer of nutrients to the periphery during the process of encystation. | 1995 | 7797363 |
| serum antibody detected by fluorescent antibody test in patients with symptomatic blastocystis hominis infection. | 1995 | 7501905 | |
| histochemical detection of carbohydrates of blastocystis hominis. | the carbohydrates of blastocystis hominis were detected by histochemical techniques using light and electron microscopy. b. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-schiff (pas) or alcian blue (ab). intense pas reactions were observed in cells fixed with glutaraldehyde or 1/2 karnovsky fixative. the cells fixed with other fixatives showed weak or no reactions with pas staining. similar results were seen in the case of ab stain. ... | 1995 | 7537145 |
| prevalence of intestinal parasitic infection in five farms in holambra, são paulo, brazil. | a parasitological survey was carried out on 222 inhabitants of five farms in holambra, located 30 km north of campinas, são paulo, brazil, on october 1992. approximately 70% of the inhabitants were found to be infected with at least one species of intestinal parasite. the positive rates of 6 helminths and 7 protozoan species detected are as follows: 5.4% ascaris lumbricoides; 8.6% trichuris trichiura; 19.8% necator americanus; 10.4% strongyloides stercoralis; 1.4% enterobius vermicularis; 0.9% h ... | 1995 | 7569635 |
| is blastocystis hominis a cause of diarrhea in travelers? a prospective controlled study in nepal. | although the pathogenicity of blastocystis hominis has been extensively debated in the medical literature, controlled studies of the association between b. hominis and diarrhea are lacking. we conducted a case-control study among expatriates and tourists in kathmandu, nepal, in which we compared the prevalence of the organism among patients with diarrhea to that among a control group without diarrhea. b. hominis was detected in 56 (30%) of 189 patients with diarrhea, compared with 40 (36%) of 11 ... | 1995 | 7578767 |
| bacteria, viruses, yeasts and protozoans associated with diarrheal disease in singapore. | labile toxin producing enterotoxic e. coli (etec) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in singapore. salmonellas ranked a close second in children (19%). other bacterial pathogens were isolated from less than 5% of subjects. blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. cryptosporidium was not detected at all. an analysis of yeast counts in smears of diarrheal and ... | 1995 | 7603751 |
| occurrence of giardia lamblia in children of municipal day-care centers from botucatu, são paulo state, brazil. | considering that the number of day-care centers for pre-school-age children has expanded rapidly in developing countries, and that these institutions presenting conditions that facilitate the transmission of many enteric agents, a parasitological survey was carried out in three municipal day-cares from botucatu: two in the urban area (one in downtown area and the other one in the city periphery area) and the third in the rural area. three separate stool specimens were collected from 147 children ... | 1995 | 8731262 |
| [blastocystis hominis, a potentially pathogenic parasite frequently identified after a travel in tropical regions]. | 1995 | 8539222 | |
| polyparasitism in two rural communities with endemic schistosoma mansoni infection in machakos district, kenya. | formol-ether concentration supplemented by fresh saline smears was used to study intestinal parasites in two communities, miu and kitengei, in machakos district, kenya. these communities differed markedly in schistosome associated morbidity, in spite of similar prevalence and intensities of infection as revealed by kato examination, seven helminth and nine protozoan species were detected among 1011 samples examined. more than 60% of the subjects were infected with more than one parasite: one had ... | 1995 | 8544228 |
| a comparative morphology of blastocystis hominis cysts with and without the "fibrillar layer". | 1995 | 9139399 | |
| elucidation of the life cycle of the intestinal protozoan blastocystis hominis. | this paper elucidates the status of the different morphological forms of blastocystis and reports the existence of thin- and thick-walled cysts in b. hominis on the basis of current experimental evidence. it is suggested that the thin-walled cysts are autoinfectious, leading to multiplication of the organism in the intestinal tract. the thick-walled cysts are responsible for external transmission via the faecal-oral route. a life cycle for b. hominis is postulated on the basis of these findings. | 1995 | 7501648 |
| light and electron microscopic localization of lipids in blastocystis hominis. | localization of the lipid of blastocystis hominis was histochemically demonstrated. b. hominis was fixed with glutaraldehyde, treated with detergents, and stained with sudan black b or nile blue. the central vacuole of b. hominis stained with sudan black b showed many black droplets with great variation in staining intensity. in nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. these results indicated the presence of neutral lipids and/or acidic ... | 1995 | 7544390 |
| ultrastructure of blastocystis hominis cysts. | blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on ficoll-paque. this procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. the cysts were examined by both transmission (tem) and scanning electron microscopy (sem). cysts were surrounded by a fibrillar layer that appeared porous in sem. many naked cysts without the fibrillar layer were also visible in sem. they were variable in shape but mo ... | 1995 | 7567903 |
| blastocystis hominis and traveler's diarrhea. | 1995 | 7578716 | |
| is there any reason to continue treating blastocystis infections? | 1995 | 7578717 | |
| enzyme-linked immunosorbent assay for detection of serum antibody to blastocystis hominis in symptomatic infections. | an enzyme-linked immunosorbent assay was devised in order to search for antibodies against blastocystis hominis in infected humans. reaction proteins were obtained from washed, axenic b. hominis cells, as sonicate. sonicate was diluted to provide 17 and 34 micrograms of protein per well. dilutions of patients' sera were applied, followed by phosphatase-conjugated goat anti-human serum and phosphatase substrate. color was measured at 405 microns wavelength. immunoglobulin g antibodies to high tit ... | 1995 | 7876972 |
| intestinal colonization of symptomatic and asymptomatic schoolchildren with blastocystis hominis. | a study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. a questionnaire was completed by each child's parents. specimens were examined by using wet-mount preparation, formaline-ether concentration, and sheather's flotation technique. trichrome and acid-fast stains were done. blastocystis hominis was observed in 203 (20.3%) of the specimens examined, and 175 specimens contained this organism in the absence of other pathog ... | 1994 | 7852590 |
| a study of the karyotypic patterns of blastocystis hominis by pulsed-field gradient electrophoresis. | 1994 | 7855129 | |
| [morphology of blastocystis hominis in feces and evaluation of parasitological methods]. | a study of the morphology of blastocystis hominis in stool in ninety-four cases humans is described as central body, ameba and granular form were found as previously described. in addition a "globulose" form as a variation of granular form is first described. the central body form (96.8%) was the most abundant form. three parasitological methods as direct microscopical examination sample with saline solution 0.85%, lugol, sudan iii, stained with quensel, iron hematoxylin and culture are evaluate ... | 1994 | 7557277 |
| morphological diversity of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples stained with iron hematoxylin. | the objective of this investigation was to study the morphological characteristics of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples. routinely processed samples were examined for morphological detail, including size, shape, nuclear detail, and central body characteristics. morphological findings revealing the importance of recognizing b. hominis in the diagnostic laboratory are described. | 1994 | 7510311 |
| differentiation of the various stages of blastocystis hominis by acridine orange staining. | acridine orange staining differentiates the cystic and the central body forms of blastocystis hominis and offers a very convenient and easy method to observe the internal structure of the parasite. acridine orange stains the nuclei and the central body of the rounded vacuolar forms of the parasite bright and dull green, respectively. the colour changes to yellow and then to flaming red-orange when the rounded central body forms of the parasite become cystic. | 1994 | 7521862 |
| ultrastructural changes during in vitro encystment of blastocystis hominis. | the morphological changes occurring in blastocystis hominis at different time points following in vitro encystment were studied by electron microscopy. the following stages of the parasite were sequentially seen: (a) the amoebic form, which was irregular in shape, with a majority of the organelles being concentrated at the condensed cytoplasmic region; (b) the pre-cystic form, which was rounded and had an electron-dense material forming a homogeneous wall around the central body; and (c) the cys ... | 1994 | 8073021 |
| four serologically different groups within the species blastocystis hominis. | 61 strains of blastocystis hominis were isolated from 340 stool specimens coming from 140 patients with intestinal symptoms and from 200 healthy persons. the overall female to male ratio was 1.9 to 1. classification of the 61 isolates of b. hominis was determined with the aid of the immunodiffusion assay. four serologically different groups could be identified. their frequency ratio in the population studied was 39.5%:39.5%:18%:3%. group 3 was found more often than the other three groups in stoo ... | 1994 | 8167435 |
| lipid biosynthesis by axenic strains of blastocystis hominis. | axenic strains of blastocystis hominis incorporated 32p, added to the medium as orthophosphate, into a number of phospholipids, including sphingomyelin, cardiolipin, phosphatidic acid, the phosphoglycerides of choline, ethanolamine, serine, and inositol and some other minor phospholipids. radioactive palmitate and glycerol provided in the growth medium introduced radiolabel into diacylglycerols, triacylglycerols, and all major phosphoglycerides found in the organism. palmitate is a major fatty a ... | 1994 | 8205379 |
| [prevalence of infection by intestinal helminths and protozoa in school children from a coastal locality in the province of valdivia, chile]. | during july-august 1989, 219 coprological samples from primary school children from niebla and los molinos localities (39 degrees 52's, 73 degrees 26'w) in the coast of province of valdivia, chile, were analysed. prevalence % (in parentheses) of infection by intestinal protozoa and helminths were the followings: entamoeba histolytica: (18.0), entamoeba coli (34.0), endolimax nana (34.4), iodamoeba buetschlii (7.4), blastocystis hominis (64.3), giardia intestinalis (27.9) chilomastix mesnili (0.8 ... | 1994 | 7654292 |
| morphology of the cyst of blastocystis hominis. | 1994 | 7667736 | |
| a comparison of direct microscopy with culture for the diagnosis of blastocystis hominis. | 1994 | 7667737 | |
| incidence of blastocystis hominis in patients with diarrhoea. | we studied the occurrence of the parasite blastocystis hominis in 1066 stool specimens from patients with diarrhoea, and investigated the relationship between the presence of b. hominis in the faeces and the age of patients. the parasite was recovered from 3.7% samples, but as the sole species of micro-organism in the stool it was recovered from 1% samples. there was no statistically significant difference in the number of b. hominis-positive stools between the younger and the older patients (p ... | 1994 | 8034994 |
| [intestinal parasitosis in the 90s: new microorganisms in new patients]. | 1994 | 8059032 | |
| a concentration technique for obtaining viable cysts of blastocystis hominis from faeces. | 1994 | 7799511 | |
| a multiple fission-like mode of asexual reproduction in blastocystis hominis. | a non-axenic and an axenic isolate of blastocystis hominis have been induced to form cysts in vitro using an encystation medium. the morphology of the parasite at different time points was observed by scanning electron microscopy. in day-2 cultures the cysts were spherical and had a non-uniform, coarse outer surface around the body. a deep, pore-like opening was seen in some of the parasites. most of the cysts from day-4 and day-6 cultures ruptured, revealing small, uniformly sized spherical bod ... | 1994 | 7809004 |
| isolation of blastocystis hominis from sewage. | 1994 | 7825017 | |
| [comparison of intestinal parasite infestation indexes among hiv positive and negative populations]. | this study was carried out on two groups of patients treated at the josé de san martín clinical hospital. one group was composed of 82 hiv-seropositive patients with no signs of diarrhea, and another one of 300 patients, not suspected of hiv infection, was considered as "control population". stool samples were collected from each patient and examined for intestinal parasites. these determinations were carried out at the department of clinical biochemistry, in the same hospital. three specimens w ... | 1994 | 7715428 |
| blastocystis hominis infection, a cause of human diarrhea. | blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ... | 1994 | 7777913 |
| touch cytology. a quick, simple, sensitive screening test in the diagnosis of infections of the gastrointestinal mucosa. | to assess the role of touch cytology (imprint from endoscopic biopsy specimens) in the diagnosis of mucosal infections of the gastrointestinal tract, we reviewed all records and specimens of patients seen during a 30-month period. touch cytology was performed by rolling biopsy specimens on glass slides. after air fixation, a rapid staining method similar to may-grünwald-giemsa was used. the following infections and pathogens were diagnosed (in decreasing order of frequency): helicobacter pylori ... | 1994 | 7979897 |
| blastocystis hominis infection and intestinal injury. | blastocystis hominis is an enteric protozoan associated with clinical illness. to determine the prevalence of intestinal injury in patients with b. hominis infection, the authors prospectively evaluated 18 patients with b. hominis infection by endoscopy and a test of intestinal permeability. seventeen patients had gastrointestinal symptoms. colonic mucosa appeared normal by lower endoscopy in 12 of 13 patients, and was friable slightly in 1. duodenal mucosa was normal by upper endoscopy in nine ... | 1994 | 8042662 |
| invasive blastocystis hominis infection in a child. | 1994 | 8044274 | |
| [blastocystis hominis as a rare arthritogenic pathogen. a case report]. | blastocysts hominis, a protozoon considered to be a nonpathogen intestinal commensal, is now discussed to be a cause of intestinal infection under certain circumstances, e.g., immunosuppression. there are two published cases of blastocysts infection complicated by arthritis which was classified as "reactive" in one case, "infectious" in the other. we report a third case: a 46-year-old female patient developed a chronic diarrhea and oligoarthritis some days after returning from a trip to senegal. ... | 1994 | 8023590 |
| intestinal parasitism in the united states: update on a continuing problem. | to document patterns of intestinal parasitism in the united states, we analyzed results of 216,275 stool specimens examined by the state diagnostic laboratories in 1987; parasites were found in 20.0%. percentages were highest for protozoans: giardia lamblia (7.2%), entamoeba coli and endolimax nana (4.2% each), blastocystis hominis (2.6%), and entamoeba histolytica (0.9%). the most commonly identified helminths were nematodes: hookworm (1.5%), trichuris trichiura (1.2%), and ascaris lumbricoides ... | 1994 | 8024063 |
| blastocystis hominis: an old organism with a new significance. | 1993 | 8477496 | |
| [intestinal parasite infections in rural students of chiloé archipelago, x region, chile]. | in order to contribute to the knowledge of intestinal parasite infection a survey was performed in 462 primary school children from eight rural localities sited in two islands of the chiloe archipelago. stool samples and perianal scotch tape scrapings for intestinal protozoa and helminths were microscopically examined. the prevalence rates of the main species found were: ascaris lumbricoides 43.3%, trichuris trichiura 26.8%, enterobius vernicularis 20.1%, entamoeba histolytica 5.6%, giardia inte ... | 1993 | 8110371 |
| blastocystis hominis: prevalence in asymptomatic versus symptomatic hosts. | blastocystis hominis, other protozoans, and fecal leukocytes were tabulated on trichrome-stained fecal smears from 182 asymptomatic and 125 symptomatic control patients. no statistically significant difference in prevalence of b. hominis was found between the asymptomatic study group and the symptomatic control group; the clinical profile of subjects with b. hominis and those not infected was similar, and no correlation was found between the presence of b. hominis and that of fecal leukocytes. h ... | 1993 | 8515120 |
| [blastocystis infection in symptomatic patients at the regional hospital of temuco, chile]. | at the regional hospital of temuco, 670 adult and children patients, with intestinal complaints, were studied to determine the prevalence of infection by enteroparasites or commensals in a general way, and by blastocystis hominis in a particular attempt. a coproparasitological study was carried out using a modified telemann method. infection by parasites and/or commensals was demonstrated in 446 (66.6%) patients. the most frequent agent was b. hominis (35.8%) followed by giardia intestinalis (22 ... | 1993 | 8110370 |
| blastocystis hominis and blastocystosis (zierdt-garavelli disease). | 1993 | 8428021 | |
| axenic culture of blastocystis hominis in iscove's modified dulbecco's medium. | 1993 | 8278347 | |
| diagnosis by faecal culture of dientamoeba fragilis infections in australian patients with diarrhoea. | this paper reports the first survey in australia to use faecal culture to detect dientamoeba fragilis in patients with diarrhoea. of 3 different protozoal culture media evaluated on a case of known infection, modified boeck & drbohlav's medium was the most suitable. the organism could be grown from faeces stored for up to 24 h at room temperature, but for only 10 h at 4 degrees c. culture was then used, in combination with microscopy of smears fixed with polyvinyl alcohol and trichrome-stained, ... | 1993 | 8337717 |