Publications

biochemical studies on the phlebotomus fever group viruses (bunyaviridae family).	analyses of the virion polypeptides and genomes of several phlebotomus fever group viruses, karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses, have established that they are biochemically similar to the accepted members of the bunyaviridae family. like snowshoe hare virus (a member of the california serogroup of the bunyavirus genus of the bunyaviridae family), karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses all have three viral rna s ...	1979	225523
discontinuous counter-immunoelectrophoresis in the study of viruses.	a method for the use of the discontinuous counter-immunoelectropnoresis (dcie) technique in the study of viruses of the major taxonomic groups is reported. the method was successfully employed in detection of antigen of viruses in the bunyavirus, bunyavirus-like, flavivirus, orbivirus, and rhabdovirus groups, and/or of antibody to these agents. although less sensitive than the complement fixation test, the dcie test was found to be rapid and in some cases more specific in differentiating related ...	1978	97243
induction of interferon by group c arboviruses. brief report.		1978	104697
investigations on the presence of antibodies to alphaviruses, flaviviruses, bunyavirus and kemerovo virus in humans and some domestic animals.	the presence of antibodies to some alphaviruses, flaviviruses, bunyaviruses and orbiviruses was studied in the sera from humans and domestic animals of biotopes "c" and "t" in the south-east of romania. specific antibodies were found in humans to 3 alphaviruses (eastern equine encephalitis, western equine encephalitis and sindbis), in a very low proportion, and to 3 flaviviruses (tickborne encephalitis, west nile and ntaya), at a much higher percentage. very low percentages of antibodies to buny ...	1978	209613
khasan virus, a new ungrouped bunyavirus isolated from haemaphysalis longicornis ticks in the primorie region.	strain leiv-776p, possessing no haemagglutinating activity was isolated in 1971 in the primorie region (u.s.s.r.) from haemaphysalis longicornis neumann 1091 tick. complement fixation (cf) tests revealed no antigenic relationship with 24 antigenic groups of arboviruses or 21 ungrouped viruses isolated from ticks. the virus isolate contains rna and is relatively sensitive to ether and sodium deoxycholate; it is pathogenic for suckling mice and two-week-old white mice by the intracerebral route, a ...	1978	27977
experimental infection of putorius eversmanni polecats and martes foina martens with tahyna virus.	three putorius eversmanni pole-cats and two martes foina martens aged about 9 months were subcutaneously infected with about 260 suckling mouse ld50 of the extraneurally passaged "236" strain of tahyna virus (california group, genus bunyavirus). viraemia with maximal titres of 1.32 (pole-cats) and 1.28 (martens) dex intraperitoneal (i.p.) mouse ld50/0.02 ml was demonstrated from 48 to 96 hr after inoculation (p.i.). by the plaque-reduction neutralization test, seroconversion was demonstrated 15 ...	1978	35949
razdan virus, a new ungrouped bunyavirus isolated from dermacentor marginatus ticks in armenia.	a virus, designated razdan, was isolated from dermacentor marginatus ticks in the armenian s.s.r. in 1973. the complement fixation tests revealed no antigenic relationships to 74 tick-borne arboviruses. the size of the virus is about 100 nm; it agglutinates goose erythrocytes at ph 5.5--7.0, is pathogenic for newborn, 14-day-old and adult white mice and multiplies in primary and continuous cell cultures. morphological properties of the virus permit its classification as a member of the family bu ...	1978	35950
antibody response to arboviruses. absence of increased response in amyotrophic lateral sclerosis and multiple sclerosis.	complement fixation and hemagglutination imhibition tests were conducted on the serums of patients with amyotrophic lateral sclerosis and multiple sclerosis using a variety of arboviral antigens. seventy-eight complement fixation and 15 hemagglutination-inhibition viral antigens were used representing togaviruses, orbiviruses, rhadoviruses, bunyaviruses, arenaviruses, and several ungrouped agents. the serological results did not indicate any relationship between these viruses and either amyotrop ...	1978	666595
transmission of northway and st. louis encephalitis viruses by arctic mosquitoes.	transmission of a canadian arctic isolate of northway virus has been demonstrated after incubation of arctic aedes communis mosquitoes at 13 degrees c for 27 days after intrathoracic injection of 300 plaque forming units of virus. replication has also been demonstrated after intrathoracic injection of domestic a. aegypti mosquitoes of this virus. virions of northway virus, 84--92 nm diameter were morphologically typical of a bunyavirus after propagation in salivary glands of a. communis or in ti ...	1978	687117
small viral rna segment of bunyaviruses codes for viral nucleocapsid protein.	tryptic peptide analyses have been undertaken on the nucleocapsid (n) protein of snowshoe hare (ssh) and la crosse (lac) bunyaviruses. similar analyses have been performed on the n proteins of two recombinant viruses which have the large/medium/small rna genome configurations: ssh/lac/lac and ssh/lac/ssh. the results provide conclusive evidence that the s rna of bunyaviruses codes for the the viral n protein.	1978	702656
serological comparison of aino and samford viruses in simbu group of bunyaviruses.		1978	739909
genetic capabilities of bunyaviruses.		1978	752701
seriologic and vector comparisons of rift valley virus with other bunyaviruses.		1978	752702
congenital abnormalities in newborn lambs after infection of pregnant sheep with akabane virus.	akabane virus (a bunyavirus) has been associated with epizootics of congenital deformities in cattle, sheep, and goats. experimental studies using mouse-adapted virus inoculated intravenously into pregnant sheep gave an inapparent infection. neutralizing antibodies were detected on day 5, and peaks in the titer were seen at days 10 and 48. ewes infected at day 30 to 36 of pregnancy produced five (31% incidence) deformed lambs. sera from four of these possessed neutralizing antibodies to akabane ...	1977	832900
the virus particle nucleic acids and proteins of four bunyaviruses.	the structural polypeptides of five bunyaviruses, snowshoe hare, lumbo and la crosse viruses (members of the california encephalitis subgroup of bunyaviruses), bunyamwera and main drain viruses (members of the bunyamwera subgroup of bunyaviruses), have been compared by polyacrylamide-sds gel electrophoresis. each virus was found to possess three major structural polypeptides, two glycoproteins (g1 and g2), and one nucleocapsid protein (n). although the sizes of the g1 polypeptides (mol. wt. appr ...	1977	839199
electron microscopy of the segmented rna genome ofla crosse virus: absence of circular molecules.	the three species of single-stranded rna present in la crosse virus were examined in the electron microscope. because large amounts of contaminating cellular dna are copurified with the virus despite extensive attempts to purify the virus, it was necessary to use procedures that eliminated the bulk of this dna before the viral rna was analyzed. when this was done, the modal lengths of la crosse virus rna were 0.4, 2.0, and 3.1 mum. these lengths correspond well to their known molecular weights o ...	1977	853565
immune electron microscopy of arboviruses.	representative members of four arbovirus taxons were studied by immune electron microscopy (iem) in an attempt to determine whether the technique can be applied to the specific identification of arboviruses. alphavirus, flavivirus, bunyavirus, and rhabdovirus antigens were prepared by propagation in tissue culture. the viruses were used to titrate homologous antibody and test heterologous antibody in the iem test. specific clumping together with visualization of antibody attachment was observed ...	1977	889020
bunyavirus isolations from mosquitoes in the western canadian arctic.	strains of california encephalitis virus (snowshoe hare subtype) were isolated from 8 of 475 pools comprising 23 747 unengorged female mosquitoes of five species collected at three of six locations throughout the mackenzie valley of the northwest territories, canada, from latitudes 60 to 69 degrees n between 10 and 24 july 1976. minimum field infection rates included 1:2734 for aedes communis, 1:256 to 1:3662 for a. hexodontus and 1:911 to 1:1611 for a. punctor. northway virus was also isolated ...	1977	18541
three unique viral rna species of snowshoe hare and la crosse bunyaviruses.	two-dimensional gel electrophoreses of rnase t1-derived oligonucleotides of the three individual rna segments of the bunyavirus snowshow hare virus indicate that its three rna segments possess distinct nucleotide sequences. the fingerprints of the rna species of snowshoe hare virus differ from those of the antigenically closely related la crosse virus. three viral rna species have been identified in preparations of melao and trivittatus as well as snowshoe hare, lumbo, and la crosse bunyaviruses ...	1977	559104
formation of recombinants between snowshoe hare and la crosse bunyaviruses.	wild-type recombinants were obtained at high frequency from coinfections of bhk cells involving temperature-sensitive, conditional-lethal mutants of snowshoe hare (ssh) and la crosse (lac) bunyaviruses. analyses of two of the recombinants indicated that they have the genome compositions ssh/lac/ssh and ssh/lac/lac for their respective l, m, and s virion rna species. this evidence, together with that for the genetic stability of the recombinants, indicates that they were derived by segment reasso ...	1977	592468
california encephalitis virus proliferation in yukon mosquitoes incubated at low temperatures.	replication of a subarctic bunyavirus, california encephalitis (snowshoe hare subtype), was detected in salivary glands and thoraces of wild-caught aedes communis mosquitoes from the yokon territory, after intrathoracic inoculation with 0.1 to 100 mouse ld50 virus, and incubation for 7 to 21 days throughout their viable temperature range of 0 to 23 degrees c. immunoperoxidase staining confirmed that viral replication occurred in the cytoplasm of acinar cells of salivary glands, both by ligh micr ...	1976	963625
structural proteins of la crosse virus.	preparations of la crosse virus, a member of the california encephalitis group of bunyaviruses, were found to possess three major virion proteins. two of the proteins were glycosylated (g1 and g2) and were located on the surface of the virus particles. these two glycoproteins were present in equimolar amounts and possessed apparent molecular weights of 120 x 10(3) and 34 x 10(3). virion nucleocapsids, isolated by a nonionic detergent and salt treatment, contained another major protein, n (molecu ...	1976	972436
recombination and complementation between temperature-sensitive mutants of a bunyavirus, snowshoe hare virus.	complementation and recombination have been observed with temperature-sensitive mutants of bunyavirus snowshoe hare virus.	1976	978796
[demonstration of circular ribonucleic acid in the lumbo virus (bunyavirus)].	electron microscopy study of rna extracted from lumbovirus shows the existence of circular molecules, which are not susceptible to thermal or chemical denaturation. these facts suggest that the rna is present inside the virion in a covalently closed circular form.	1976	820471
the first isolations of eastern encephalitis, group c, and guama group arboviruses from the peruvian amazon region of western south america.	two strains of eastern encephalitis (ee) virus were isolated in the amazon region of peru near pucallpa, loreto department, using sentinel hamsters. ee virus antibodies were found in healthy horses at both pucallpa and iquitos in the same department. fourteen group c and four guama group arboviruses were recovered from sentenel hamsters and mosquitoes near iquitos. the group c agents were caraparu-ossa, marituba, and oriboca-itaqui viruses, and the guama group agents were bimiti virus. besides p ...	1975	238693
the structure of togaviruses and bunyaviruses.	this article reviews the properties of arthropod-borne and nonarbo togaviruses and members of the bunyaviridae family. averaged information is given on the morphology and substructure of the virion, its physical properties, its chemical composition and the function of its constituents. special attention is given to distinctive traits which might be useful for virus classification.	1975	1107688
the structure of uukuniemi virus, a proposed member of the bunyaviruses.	uukuniemi virus, a proposed member of the new large bunyavirus group of arboviruses, has an interesting structure which differs in many respects from that of other enveloped rna viruses. the surface structure reveals a clustering of the two glycoproteins into distinct capsomere-like subunits, arranged in a t = 12 icosahedral lattice. the genome is found in at least three pieces that appear to represent unique virus-specific rnas. the ribonucleoproteins corresponding to the rna species are circul ...	1975	1107689
the basis of arbovirus classification.	the biologically defined set of arboviruses contains well over 300 separate viruses which have been subdivided into some 40 serological groups on the basis of antigenic cross-reactivity. more than three quarters of all arboviruses can now bw placed into one of the following five major taxonomic genera based upon the fundamental properties of the virion: alphavirus, flavivirus, orbivirus, rhadovirus, bunyavirus. there are 20 alphaviruses, representing serological group a, and 57 flaviviruses in s ...	1975	1207193
bunyaviruses and bunyaviridae.	a new family is described, the bunyaviridae, which contains a single genus, bunyavirus. the main characteristics of the family are as follows: single-stranded rna, total molecular weight about 7 x 10(6) daltons, probably in three segments. virions spherical, enveloped particles 90-100 nm in diameter. envelope contains at least one virus-specified glycopeptide. develop in the cytoplasm, mature by budding into smooth-surfaced vesicles in the golgi region or nearby. internal ribonucleoprotein compo ...	1975	1235823
[rna polymerase activity associated with a bunya virus (lumbo)].	rna dependent rna polymerase has been demonstrated in purified lumbo virus (bunyavirus) which contains a single stranded segmented rna. divalent cations (mn++ and mg++) are required for optimal in vitro activity. reaction products can be specifically annealed with the viral genome.	1975	808281
[ultrastructure, development and nucleic acid of the s virus from the decapod crustacea macropipus depurator l].	the "s" crabe virus recently isolated from macropipus depurator (decapoda) has been purified and its nucleic acid has been recognized as a dna. the visualization of the structure and of several phases of the cycle of the virus permits to situate this virus near the bunyavirus and paramyxovirus groups.	1975	808338
[circular ribonucleoproteins in the virus lumbo (bunyavirus)].		1975	808354
mosquito-borne arboviruses in arctic america.	mosquito-borne arboviruses are prevalent throughout subarctic regions of canada and alaska, principally in the boreal forest extending between latitudes 53 and 66 degrees n, but they have been identified in tundra regions as far north as 70 degrees n. all mosquito-borne agents have been bunyaviruses, comprising principally the snowshoe hare subtype of california encephalitis (ce) virus, but also northway virus. mosquito vectors comprise several aedes species and culiseta inornata, all of which h ...	1975	1602
bunyaviruses and bunyaviridae.		1974	4850836
three-segment rna genome of lumbo virus (bunyavirus).		1973	4210363