Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| two novel neutralizing antigenic epitopes of the s1 subunit protein of a qx-like avian infectious bronchitis virus strain sczy3 as revealed using a phage display peptide library. | the spike (s) protein of the infectious bronchitis virus (ibv) plays a central role in the pathogenicity, the immune antibody production, serotype and the tissue tropism. in this study, we generate 11 monoclonal antibodies (mabs) against s1 subunit of ibv sczy3 strain, and two mabs 1d5 and 6a12 were positive in indirect elisa against both his-s1 protein and the purified whole viral antigen. mab 6a12 and 1d5 could recognized by other 10 ibv strains (ibvs) from five different genotypes, except tha ... | 2015 | 26315775 |
| immune responses to virulent and vaccine strains of infectious bronchitis viruses in chickens. | infectious bronchitis (ib) is an acute and highly contagious chicken viral disease, causing severe economic losses to poultry producers worldwide. in the last few decades, infectious bronchitis virus (ibv) has been extensively studied, but knowledge of immune responses to virulent or vaccine strains of ibvs remains limited. this review focuses on fundamental aspects of immune responses against ibv, including the role of pattern recognition receptors (prrs) in identification of conserved viral st ... | 2015 | 26301315 |
| effects of adaptation of infectious bronchitis virus arkansas attenuated vaccine to embryonic kidney cells. | the population structure of an embryo-attenuated infectious bronchitis virus (ibv) arkansas (ark) delmarva poultry industry (dpi)-derived vaccine was characterized during serial passages in chicken embryo kidney (cek) cells and after back-passage in embryonated chicken eggs (ece) and in chickens. both conventional and deep-sequencing results consistently showed population changes occurred during adaptation to cek cells. specifically, 13 amino acid (aa) positions seemed to be targets of selection ... | 2015 | 26292543 |
| safety and efficacy of an attenuated chinese qx-like infectious bronchitis virus strain as a candidate vaccine. | infectious bronchitis (ib) is a highly contagious respiratory and urogenital disease of chickens caused by infectious bronchitis virus (ibv). this disease is of considerable economic importance and is primarily controlled through biosecurity and immunization with live attenuated and inactivated ib vaccines of various serotypes. in the present study, we tested the safety and efficacy of an attenuated predominant chinese qx-like ibv strain. the results revealed that the attenuated strain has a cle ... | 2015 | 26277655 |
| genetic characterization of the belgian nephropathogenic infectious bronchitis virus (nibv) reference strain b1648. | the virulent nephropathogenic infectious bronchitis virus (nibv) strain b1648 was first isolated in 1984, in flanders, belgium. despite intensive vaccination, b1648 and its variants are still circulating in europe and north africa. here, the full-length genome of this belgian nibv reference strain was determined by next generation sequencing (ngs) to understand its evolutionary relationship with other ibv strains, and to identify possible genetic factors that may be associated with the nephropat ... | 2015 | 26262637 |
| phylogenetic analysis of avian infectious bronchitis virus s1 glycoprotein regions reveals emergence of a new genotype in moroccan broiler chicken flocks. | infectious bronchitis virus (ibv), a major pathogen of commercial poultry flocks, circulates in the form of several serotypes/genotypes. only a few amino-acid changes in the s1 subunit of wild-type ibvs proteins may result in mutants unaffected by current vaccines. | 2015 | 26239707 |
| genotypes of infectious bronchitis viruses circulating in the middle east between 2009 and 2014. | we are reporting on the infectious bronchitis virus (ibv) genotypes circulating within seven middle east countries and the alterations in genotype distributions between 2009 and 2014. tissue samples on fta cards were received over the six-year period. viral rna was extracted using phenol chloroform and subjected to nested rt-pcr targeting a 393 bp region of the s1 gene before being followed by sequencing. from the 461 submitted samples, 363 were ibv positive by rt-pcr (77.01%). of these, 355 (97 ... | 2015 | 26226233 |
| combined infectious bronchitis virus arkansas and massachusetts serotype vaccination suppresses replication of arkansas vaccine virus. | polyvalent infectious bronchitis virus vaccination is common worldwide. the possibility of vaccine interference after simultaneous combined vaccination with arkansas (ark) and massachusetts (mass)-type vaccines was evaluated in an effort to explain the high prevalence of ark-type infectious bronchitis virus in vaccinated chickens. chickens ocularly vaccinated with combinations of ark and mass showed predominance of mass vaccine virus before 9 days post-vaccination (dpv) in tears. even when mass ... | 2015 | 26223977 |
| successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain. | a natural recombinant nephropathogenic k40/09 strain of infectious bronchitis virus (ibv) was heat-adapted for possible future use as live attenuated vaccine. the k40/09 strain was selected during successive serial passages in specific-pathogen free (spf) embryonated eggs at sub-optimal higher temperature (56°c). unlike the parental strain, the attenuated strain, designated k40/09 hp50, was found to be safe in 1-day-old spf chicks, which showed neither mortality nor signs of morbidity, and rarel ... | 2015 | 26218899 |
| mechanistic study of intertypic nucleoprotein complex formation and its inhibitory effect toward influenza a virus. | co-infection of influenza a and b viruses (iav and ibv) results in marked decreases in iav replication. multiple mechanisms have been proposed for this phenomenon. recently, we reported that ibv nucleoprotein (bnp) alone can suppress iav replication and proposed an inhibition model in which bnp binds iav nucleoprotein (anp) and disrupts iav polymerase complexes. here, using mutagenesis and co-immunoprecipitation, we determined the protein motifs mediating the intertypic anp-bnp complex and showe ... | 2015 | 26218215 |
| mucosal, cellular, and humoral immune responses induced by different live infectious bronchitis virus vaccination regimes and protection conferred against infectious bronchitis virus q1 strain. | the objectives of the present study were to assess the mucosal, cellular, and humoral immune responses induced by two different infectious bronchitis virus (ibv) vaccination regimes and their efficacy against challenge by a variant ibv q1. one-day-old broiler chicks were vaccinated with live h120 alone (group i) or in combination with cr88 (group ii). the two groups were again vaccinated with cr88 at 14 days of age (doa). one group was kept as the control (group iii). a significant increase in l ... | 2015 | 26202435 |
| molecular investigation of a full-length genome of a q1-like ibv strain isolated in italy in 2013. | since 1996 a new infectious bronchitis virus (ibv) genotype, referred to as q1, circulated in china and was reported for the first time in italy in 2011, associated with an increase of mortality, kidney lesions and proventriculitis. during northern italian outbreak of respiratory disease in a broiler flock in 2013, an ibv strain was detected by rt-pcr and characterized as q1-like based on partial s1 sequence. the virus was isolated and named γcov/ck/italy/i2022/13. all coding regions of the isol ... | 2015 | 26191621 |
| increased level of protection of respiratory tract and kidney by combining different infectious bronchitis virus vaccines against challenge with nephropathogenic brazilian genotype subcluster 4 strains. | genotyping of seven infectious bronchitis virus (ibv) strains isolated in brazil showed that all belonged to the common brazilian genotype and that these strains were closest to the subcluster of strain ibv/brazil/2007/usp-19. pathotyping of four selected brazilian strains showed that they all caused a considerable level of ciliostasis in the trachea but at a somewhat lower level than did m41 and brazilian strains 50/96, 57/96, 62/96 and 64/96 representing four different serotypes that had been ... | 2015 | 26181294 |
| differential modulation of avian β-defensin and toll-like receptor expression in chickens infected with infectious bronchitis virus. | the host innate immune response either clears invading viruses or allows the adaptive immune system to establish an effective antiviral response. in this study, both pathogenic (passage 3, p3) and attenuated (p110) infectious bronchitis virus (ibv) strains were used to study the immune responses of chicken to ibv infection. expression of avian β-defensins (avbds) and toll-like receptors (tlrs) in 16 tissues of chicken were compared at 7 days pi. the results showed that p3 infection upregulated t ... | 2015 | 26142390 |
| a coronavirus e protein is present in two distinct pools with different effects on assembly and the secretory pathway. | coronaviruses (covs) assemble by budding into the lumen of the early golgi complex prior to exocytosis. the small cov envelope (e) protein plays roles in assembly, virion release, and pathogenesis. cov e has a single hydrophobic domain (hd), is targeted to golgi complex membranes, and has cation channel activity in vitro. however, the precise functions of the cov e protein during infection are still enigmatic. structural data for the severe acute respiratory syndrome (sars)-cov e protein suggest ... | 2015 | 26136577 |
| dynamic changes of virus load in supernatant of primary cek cell culture infected with different generations of avian infectious bronchitis virus strains sczy3 as revealed by real-time reverse transcription-polymerase chain reaction. | infectious bronchitis virus (ibv) can multiply effectively in chick embryo kidney (cek) cells after adapting to the chick embryo. to investigate the dynamic changes in ibv load in the supernatant of primary cek cells, we developed an sybr green i-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the ibv-sczy3 strain. the 20, 54, and 87th generations of cek-adapted ibv-sczy3 strains were used to infect cek cells, and then nucleic copy numbers in the samples of su ... | 2015 | 26125838 |
| elevated level of renal xanthine oxidase mrna transcription after nephropathogenic infectious bronchitis virus infection in growing layers. | to assess relationships between xanthine oxidase (xod) and nephropathogenic infectious bronchitis virus (nibv) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. each chicken in the control and infected group was intranasally inoculated with 0.2 ml sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal xod mrna expression in growing layers were evaluated at 8, 15 and 22 d ... | 2015 | 26119168 |
| decreased neutralizing antigenicity in ibv s1 protein expressed from mammalian cells. | we evaluated the antigenicity of recombinant infectious bronchitis virus (ibv) s1 protein expressed in mammalian cells. recombinant s1 was expressed as a secreted protein fused with a trimerization motif peptide, then purified using ni sepharose. the purified protein was analyzed by western blotting, mixed with oil adjuvant, and administered to 29-day-old specific-pathogen-free chickens. six weeks after immunization, anti-ibv neutralizing titer and anti-s1 elisa titer were determined; immunized ... | 2015 | 26113306 |
| neutralization analysis of a chicken single-chain variable fragment derived from an immune antibody library against infectious bronchitis virus. | avian infectious bronchitis virus (ibv), which is prevalent in many countries causing severe economic loss to the poultry industry, causes infectious bronchitis (ib) in birds. recombinant single-chain variable fragments (scfvs) have been proven to effectively inhibit many viruses, both in vitro and in vivo, and they could be a potential diagnostic and therapeutic reagent to control ib. in this study, six anti-ibv chicken scfvs, zl.10, zl.64, zl.78, zl.80, zl.138, and zl.256, were obtained by scr ... | 2015 | 26090700 |
| novel receptor specificity of avian gammacoronaviruses that cause enteritis. | viruses exploit molecules on the target membrane as receptors for attachment and entry into host cells. thus, receptor expression patterns can define viral tissue tropism and might to some extent predict the susceptibility of a host to a particular virus. previously, others and we have shown that respiratory pathogens of the genus gammacoronavirus, including chicken infectious bronchitis virus (ibv), require specific α2,3-linked sialylated glycans for attachment and entry. here, we studied deter ... | 2015 | 26063435 |
| survey indicates circulation of 4/91 and qx-type infectious bronchitis viruses in hungary in 2014 - short communication. | understanding the epidemiology and improving vaccinal protection against the highly variable chicken infectious bronchitis virus (ibv) requires the knowledge of circulating ibv serotypes/genotypes in defined geographic areas. accordingly, the authors initiated a survey among the major poultry producers in hungary in order to reveal the prevailing ibv serotypes in the country. tracheal swabs and organ samples (caecal tonsils, kidneys, and trachea) were collected from broiler, layer, and meat-type ... | 2015 | 26551428 |
| assessing the economic burden of avian infectious bronchitis on poultry farms in brazil. | avian infectious bronchitis (ib), caused by avian infectious bronchitis virus (ibv), is a worldwide endemic disease of chickens that affects all branches of the poultry industry. multiple geno/serotypes occur, and lowvaccine cross-protection results from the highly divergent ibv types. in view of the lack of consistent data on the economic losses caused by ib and the poor protection resulting from the use of the massachusetts type as a live vaccine in brazil, this survey aimed to estimate the lo ... | 2015 | 27044167 |
| viral quantity and pathological changes in broilers experimentally infected by irfibv32 isolate of infectious bronchitis virus. | an iranian isolate of avian infectious bronchitis virus irfibv32 was quantified in experimentally infected broilers using real-time reverse transcriptase polymerase chain reaction and histopathological changes was investigated. thirty-six 3-week-old commercial broilers were inoculated by 10(5) eld50/0.1 ml of the virus. on the various days post inoculation (dpi) different tissues were collected. the virus strongly started the replication in trachea at 1 dpi and reached to the maximum titer at 3 ... | 2015 | 26645044 |
| cross-protection by infectious bronchitis viruses under controlled experimental conditions. | infectious bronchitis virus (ibv) cross-protection trials were performed in healthy chickens maintained under controlled environmental conditions. chickens primed or primed and boosted with a massachusetts (mass)-type attenuated vaccine were subsequently challenged with either ibv arkansas (ark) or ga13-type virulent strains. in addition, ark-vaccinated chickens were challenged with ibv ga13. spike protein 1 (s1) amino acid identities between ibv vaccine and challenge strains varied from 76.0% t ... | 2015 | 26629628 |
| effect of different levels of dietary sweet orange (citrus sinensis) peel extract on humoral immune system responses in broiler chickens. | this experiment was conducted to evaluate the effects of different levels of sweet orange (citrus sinensis) peel extract (sope) on humoral immune system responses in broiler chickens. three hundred 1-day broilers (ross-308) were randomly allocated to treatments varying in supplemental sope added in the drinking water. the experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of sope and a treatment ... | 2015 | 24990585 |
| gexp analyzer-based multiplex reverse-transcription pcr assay for the simultaneous detection and differentiation of eleven duck viruses. | duck viral pathogens primarily include the avian influenza virus (aiv) subtypes h5, h7, and h9; duck hepatitis virus (dhv); duck tembusu virus (dtmuv); egg drop syndrome virus (edsv); duck enteritis virus (dev); newcastle disease virus (ndv); duck circovirus (ducv); muscovy duck reovirus (mdrv); and muscovy duck parvovirus (mdpv). these pathogens cause great economic losses to china's duck breeding industry. | 2015 | 26518004 |
| comparative evaluation of in vitro and in vivo assays for the detection of avian infectious bronchitis virus as a contaminant of live poultry vaccines. | a reverse transcriptase polymerase chain reaction (rt-pcr( assay specific for identifying avian infectious bronchitis virus (ibv) in poultry vaccines, and the serological response to ibv induced by the inoculation of chicks with a newcastle disease vaccine spiked with the massachusetts strain of ibv, were compared for their ability to detect ibv as a contaminant of avian vaccines. the sensitivity of the ibv-rt-pcr assay provided results which were at least equivalent to the biological effect pro ... | 2015 | 26042490 |
| the effect of estrogen on the early cytotoxic response to ib virus infection in hen oviduct. | the aim of this study was to determine whether the egg-laying phase and estrogen affect the induction of cytotoxic cells in response to avian infectious bronchitis (ib) virus at early stage of infection in the oviduct. attenuated ib virus (aibv group) or its vehicle (control group) was introduced to the oviductal magnum lumen of white leghorn hens in the laying and molting phase, as well as molting hens injected with estradiol benzoate (m-eb hens) or corn oil (m-oil hens). oviductal isthmus and ... | 2015 | 25593044 |
| progress and challenges toward the development of vaccines against avian infectious bronchitis. | avian infectious bronchitis (ib) is a widely distributed poultry disease that has huge economic impact on poultry industry. the continuous emergence of new ibv genotypes and lack of cross protection among different ibv genotypes have been an important challenge. although live attenuated ib vaccines remarkably induce potent immune response, the potential risk of reversion to virulence, neutralization by the maternal antibodies, and recombination and mutation events are important concern on their ... | 2015 | 25954763 |
| rapid detection of avian infectious bronchitis virus by reverse transcriptase-loop mediated isothermal amplification. | a reverse-transcription loop mediated isothermal amplification (rt-lamp) was developed for rapid diagnosis of infectious bronchitis (ib) in poultry by targeting the spike protein 2 gene (s2). rt-lamp primers were designed for ibv-s2 targets and optimized to run at 60 °c for 45 min. as compared with rt-pcr, rt-lamp was 100 times more sensitive for ibv-s2 gene. rt-lamp showed specific amplification with ib viral genome but not with other avian respiratory pathogens due to their mismatching with ib ... | 2015 | 32226206 |
| diverse gammacoronaviruses detected in wild birds from madagascar. | to date, infectious bronchitis virus (ibv) is potentially found in wild birds of different species. this work reports the survey of coronaviruses in wild birds from madagascar based on the targeting of a conserved genome sequence among different groups of covs. phylogenetic analyses revealed the presence of gammacoronaviruses in different species of gruiformes, passeriformes, ciconiiformes, anseriformes, and charadriiformes. furthermore, some sequences were related to various ibv strains. aquati ... | 2015 | 32214942 |
| [genotypes and serotypes of avian infectious bronchitis viruses isolated during 2009-2011 in guangxi, china]. | in order to investigate the prevalence and track genetic and antigenic evolutions of infectious bronchitis virus (ibv) and their prevalence in guangxi, china since 1985, gene amplification and sequencing and virus neutralization (vn) test on chicken embryo tracheal organ cultures were used in genotyping and serotyping of 28 ibv isolates during 2009-2011 in guangxi. the results of n gene sequencing and comparison showed that the 28 isolates and reference strains were classified into three groups, ... | 2014 | 24923170 |
| infectious bronchitis virus in different avian physiological systems-a field study in brazilian poultry flocks. | avian infectious bronchitis is a highly contagious viral disease with economic effects on poultry agribusiness. the disease presents multi-systemic clinical signs (respiratory, renal, enteric, and reproductive) and is caused by one coronavirus (infectious bronchitis virus, ibv). infectious bronchitis virus is classified into different serotypes and genotypes (vaccine strains and field variants). this study aimed to evaluate the occurrence of ibv in commercial poultry flocks from 3 important prod ... | 2014 | 24894532 |
| emergence of avian infectious bronchitis in a non-vaccinating country. | infectious bronchitis virus (ibv) is a coronavirus of the chicken. it is a highly contagious pathogen and in addition to causing respiratory and kidney diseases can affect the reproductive organs, resulting in loss of production and poor egg quality. despite the global distribution of ibv, finland has been free of clinical cases for almost three decades. since april 2011, outbreaks involving genotypes qx, d274-like and 4/91-like have occurred in southern finland. the clinical samples studied wer ... | 2014 | 24766156 |
| emergence of avian infectious bronchitis virus and its variants need better diagnosis, prevention and control strategies: a global perspective. | growth in poultry sector is being challenged due to increased incidence and re-emergence of diseases caused due to evolution of several viral pathogens and use of live vaccines. piles of economic losses are encountered due to these diseases. avian infectious bronchitis (ib), caused by corona virus, is oie-listed disease and characterized by respiratory, renal and urogenital involvements, causing high mortality. economic losses are encountered due to loss of productive performance of both egg and ... | 2014 | 26035949 |
| two genotypes of infectious bronchitis virus are responsible for serological variation in kwazulu-natal poultry flocks prior to 2012. | this study describes the isolation, serotyping and genotyping of 54 infectious bronchitis virus (ibv) cases predominantly in kwazulu-natal and compared to several isolates from other south african provinces between 2011 and 2012 and several historic isolates. the results indicate the division of isolates into two different genotypes of ibv within the province, massachusetts (mass)-like and qx-like. the ibv mass-like genotype was the most prevalent and was detected in 79% of the full spike protei ... | 2014 | 25685887 |
| effects of challenge with very virulent infectious bursal disease virus reassortants in commercial chickens. | pathogenicity and immune responses were characterized in commercial broilers and layers challenged with very virulent infectious bursal disease virus (vvibdv) reassortants (vvibdv segment a + serotype 2 segment b and vvibdv segment a + classic virulent segment b) at 7 days of age. in addition, functional immunosuppression was evaluated after challenge with infectious bronchitis virus (ibv) at 15 days of age. layers showed higher levels and increased persistence of ibdv- and ibv-specific maternal ... | 2014 | 25619003 |
| molecular detection of infectious bronchitis and newcastle disease viruses in broiler chickens with respiratory signs using duplex rt-pcr. | infectious bronchitis (ib) and newcastle disease (nd) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. in the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-rt-pcr. rna was extracted from vaccinal and reference strains of infectious bronchitis virus (ibv) and newcastle disease virus ( ... | 2014 | 25610585 |
| detection of infectious bronchitis virus with the use of real-time quantitative reverse transcriptase-pcr and correlation with virus detection in embryonated eggs. | real-time quantitative reverse transcriptase-polymerase chain reaction (qrt-pcr) assays have been used to detect the presence of challenge virus when the efficacy of infectious bronchitis virus (ibv) vaccine against field viruses is being experimentally evaluated. however, federal guidelines for licensing ibv vaccines indicate that challenge-virus detection following vaccination is to be conducted in embryonated eggs. in this study, we examined qrt-pcr data with the use of universal and type-spe ... | 2014 | 25518434 |
| continued use of ibv 793b vaccine needs reassessment after its withdrawal led to the genotype's disappearance. | over a period of almost two years, broilers chickens on several hundred italian farms, were monitored for infectious bronchitis virus. detections were genotyped using a hypervariable region of the gene coding for the s1 segment of the spike protein. a range of genotypes were detected which comprised qx, q1, mass, d274 and 793b. sequences of 793b viruses detected in chickens, vaccinated with either of the two commonly used 793b type vaccines were almost identical to sequences of one or other of t ... | 2014 | 25446828 |
| characterization of cellular and humoral immune responses after ibv infection in chicken lines differing in mbl serum concentration. | chickens from two inbred lines selected for high (l10h) or low (l10l) mannose-binding lectin (mbl) serum concentrations were infected with infectious bronchitis virus (ibv), and innate as well as adaptive immunological parameters were measured throughout the experimental period. chickens with high mbl serum concentrations were found to have less viral load in the trachea than chickens with low mbl serum concentrations indicating that these chickens were less severely affected by the infection. t ... | 2014 | 25343382 |
| factors influencing the outcome of infectious bronchitis vaccination and challenge experiments. | the factors influencing the outcome of infectious bronchitis vaccination and challenge experiments regarding the respiratory and renal systems are reviewed. advantages and disadvantages of the available techniques for measuring protection against an infectious bronchitis virus challenge are discussed, including the definition of protection itself. suggestions are made regarding some ways in which progress towards standardization of a recognized protocol for performing experimental challenge stud ... | 2014 | 25338230 |
| [rescue of the recombinant infectious bronchitis virus with the ectodomain region of h120 spike glycoprotein]. | to explore the expression potential of heterogeneous genes using the backbone of infectious bronchitis virus (ibv) beaudette strain, the ectodomain region of the spike gene (1,302 bp) of ibv h120 strain was amplified by rt-pcr and replaced into the corresponding location of the ibv beaudette strain full-length cdna. this recombinant was designated as beaur-h120(s1). beaur-h120(s1) was directly used as the dna template for the transcription of viral genomic rna in vitro. then, the transcription p ... | 2014 | 25868282 |
| [dynamic distribution of the avian infectious bronchitis virus isolate strain jin-13 in spf chickens]. | this study aimed to understand the dynamic distribution of infectious bronchitis virus (ibv) jin-13 strain in spf chickens. ninety-day-old spf chickens were inoculated with jin-13, a virulent strain, and dissected at day 1, 4, 7, 10, 14, 21, 28 or 35 post-inoculation (dpi). samples of heart, liver, spleen, lung, trachea, kidney and duodenum were collected and the n gene was detected by sybr green i real-time quantitative rt-pcr assays. the established method had a good linear correlation from 7. ... | 2014 | 25272586 |
| [complete genomic analysis of a novel infectious bronchitis virus isolate]. | the genome of ck/ch/sd09/005, an isolate of infectious bronchitis virus (ibv), was characterized to enable the further understanding of the epidemiology and evolution of ibv in china. twenty-five pairs of primers were designed to amplify the full-length genome of ck/ch/sd09/005. the nucleotide sequence of ck/ch/sd09/005 was compared with reference ibv strains retrieved from genbank. the phylogenic relationship between ck/ch/sd09/005 and the reference strains was analyzed based on s1 gene sequenc ... | 2014 | 25272584 |
| development of slide elisa (selisa) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides. | the development of an easy and simpler method of slide enzyme-linked immunosorbent assay (selisa) for the diagnosis of four economically important poultry viruses viz., newcastle disease virus (ndv), infectious bronchitis virus (ibv), infectious bursal disease virus (ibdv) and egg drop syndrome 76 virus (eds 76) and the use of selisa for semi quantitation of ndv are described. the positive signals for viral aggregates were detected under light microscope. this is the first report regarding the d ... | 2014 | 25218174 |
| genomic characteristics and changes of avian infectious bronchitis virus strain ck/ch/ldl/97i after serial passages in chicken embryos. | we previously attenuated the infectious bronchitis virus (ibv) strain ck/ch/ldl/97i and found that it can convey protection against the homologous pathogenic virus. | 2014 | 25195733 |
| recombinant nucleocapsid protein based single serum dilution elisa for the detection of antibodies to infectious bronchitis virus in poultry. | avian infectious bronchitis is ubiquitous and highly contagious disease of poultry, with profound effect on commercial poultry production. for effective control of infectious bronchitis virus (ibv), quick and specific diagnosis is of utmost importance. in this study, the virus was isolated from clinical samples from india and the full length nucleocapsid (n) gene was amplified, cloned and expressed in a prokaryotic system. the purified recombinant n protein based single serum dilution enzyme lin ... | 2014 | 25173423 |
| molecular characterization of an infectious bronchitis virus strain isolated from northern china in 2012. | this study reports the complete genome sequence of an infectious bronchitis virus (ck/ch/sd/121220, kj128295) isolated in 2012 from shandong province in northern china. the genome is 27,666 nt long, comprising six genes and 5' and 3' untranslated regions. the full-length genome of the ck/ch/sd/121220 isolate had the highest nucleotide sequence identity (96.7 %) to the yx10 strain. sites of recombination were identified in the genes 1ab, s, 5a, 5b and n, with their putative parental strains belon ... | 2014 | 25168045 |
| inflammatory and cell-mediated immune responses in the respiratory tract of chickens to infection with avian infectious bronchitis virus. | tracheal mucosa is the primary site of replication of avian infectious bronchitis virus (ibv), which leads to both morphologic and immune modulatory changes in this organ. to increase the understanding of the mechanisms involved in these processes, we focused on the evaluation of local inflammatory and cell-mediated immune responses after challenge with the m41 strain of ibv, associating these responses with pathologic changes in the tracheal mucosa. at 24 h post-infection, inflammatory cytokine ... | 2014 | 25105981 |
| varying antibody responses of laying hens housed in an aviary system and in furnished cages. | antibody titers after vaccination against infectious bronchitis virus (ibv), newcastle disease virus (ndv) and after natural infection with campylobacter were analyzed in five trials with lohmann silver laying hens kept in two different housing systems. in these studies it could be demonstrated that antibodies against ibv and campylobacter were in 4 out of 5 respectively in 2 out of 5 trials significantly higher in hens housed in an aviary system compared to those kept in furnished cages. the op ... | 2014 | 25080819 |
| s1 of distinct ibv population expressed from recombinant adenovirus confers protection against challenge. | protective properties of three distinct infectious bronchitis virus (ibv) ark delmarva poultry industry (arkdpi) s1 proteins encoded from replication-defective recombinant adenovirus vectors were investigated. using a suboptimal dose of each recombinant virus, we demonstrated that ibv s1 amino acid sequences showing > or = 95.8% amino acid identity to the s1 of the challenge strain differed in their ability at conferring protection. indeed, the s1 sequence of the ibv population previously design ... | 2014 | 25055623 |
| cpg-odns induced changes in cytokine/chemokines genes expression associated with suppression of infectious bronchitis virus replication in chicken lungs. | the process of virus replication in host cells is greatly influenced by the set of cytokines, chemokines and antiviral substances activated as a result of host-virus interaction. alteration of cytokines profiles through manipulation of the innate immune system by innate immune stimulants may be helpful in inhibiting virus replication in otherwise permissive cells. the aim of present studies was to characterize innate immune responses capable of inhibiting infectious bronchitis virus (ibv) replic ... | 2014 | 25012000 |
| analysis of s1 gene of avian infectious bronchitis virus isolated in southern china during 2011-2012. | sixty-two strains of avian infectious bronchitis virus (ibv) were isolated from diseased chickens at different farms in southern china during 2011-2012, and 66.1 % of the isolated strains were associated with typical nephritis. analysis of the s1 gene sequences amplified from the 62 isolated strains together with 40 reference strains published in genbank showed nucleotide homologies ranging from 63.5 to 99.9 % and amino acid homologies ranging from 57.9 to 100 %. phylogenetic analysis revealed t ... | 2014 | 25000989 |
| regulation of coronaviral poly(a) tail length during infection is not coronavirus species- or host cell-specific. | it has been demonstrated that the length of the poly(a) tail in the bovine coronavirus (bcov), which belongs to genus betacoronaviruses, is regulated throughout infection in human rectal tumor-18 (hrt-18) cells, and the length of the poly(a) tail is associated with the efficiency of virus translation. here, we examined whether the regulation of viral poly(a) tail length is cell-type independent and whether it is a common feature of coronaviruses to assess the significance of the regulation. by l ... | 2014 | 25034371 |
| emergence of enteric viruses in production chickens is a concern for avian health. | several viruses have been identified in recent years in the intestinal contents of chickens and turkeys with enteric problems, which have been observed in commercial farms worldwide, including brazil. molecular detection of these viruses in brazil can transform to a big threat for poultry production due to risk for intestinal integrity. this disease is characterized by severely delayed growth, low uniformity, lethargy, watery diarrhea, delayed feed consumption, and a decreased conversion rate. c ... | 2014 | 24578633 |
| bacmam virus-based surface display of the infectious bronchitis virus (ibv) s1 glycoprotein confers strong protection against virulent ibv challenge in chickens. | avian infectious bronchitis virus (ibv) is associated with production inefficiencies in domestic fowl, and causes massive economic losses to the poultry industry worldwide. progress has been made in designing novel and efficient candidate vaccines to control ibv infection. bacmam virus, a modified baculovirus mediating transgene expression under the control of a mammalian promoter, has emerged as a versatile and safe vector during vaccine development. in previous work, we generated the bacmam vi ... | 2014 | 24342247 |
| pathomorphological studies of polyserositis in commercial caged layer chicken. | to detect etiological agents and pathological changes associated with polyserositis in commercial layer chicken. | 2014 | 25312143 |
| simultaneous typing of nine avian respiratory pathogens using a novel gexp analyzer-based multiplex pcr assay. | a new, rapid, and high-throughput genomelab gene expression profiler (gexp) analyzer-based multiplex pcr method was developed for simultaneous detection and differentiation of nine avian respiratory pathogens. the respiratory pathogens included in this study were avian influenza subtypes h5, h7, and h9, infectious bronchitis virus (ibv), newcastle disease virus (ndv), infectious laryngotracheitis virus (iltv), mycoplasma gallisepticum (mg), mycoplasma synoviae (ms) and haemophilus paragallinarum ... | 2014 | 25025815 |
| mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus. | the infection of the avian coronavirus infectious bronchitis virus (ibv) is initiated by the binding of the spike glycoprotein s to sialic acids on the chicken host cell. in this study we identified the receptor-binding domain (rbd) of the spike of the prototype ibv strain m41. by analyzing the ability of recombinantly expressed chimeric and truncated spike proteins to bind to chicken tissues, we demonstrate that the n-terminal 253 amino acids of the spike are both required and sufficient for bi ... | 2014 | 24314633 |
| high seroprevalence of respiratory pathogens in hobby poultry. | seroprevalence studies on respiratory pathogens have been done extensively in commercial laying hens, broilers, and, to a lesser extent, backyard poultry. in europe, seroprevalence studies in backyard and fancy breed poultry flocks are scarce and limited to a few pathogens, such as mycoplasma gallisepticum (mg); others, such as ornithobacterium rhinotracheale (ort), are missing. a commercial elisa for detection of antibodies against six selected pathogens was performed on 460 serum samples from ... | 2014 | 25619008 |
| discovery of a novel bottlenose dolphin coronavirus reveals a distinct species of marine mammal coronavirus in gammacoronavirus. | while gammacoronaviruses mainly comprise infectious bronchitis virus (ibv) and its closely related bird coronaviruses (covs), the only mammalian gammacoronavirus was discovered from a white beluga whale (beluga whale cov [bwcov] sw1) in 2008. in this study, we discovered a novel gammacoronavirus from fecal samples from three indo-pacific bottlenose dolphins (tursiops aduncus), which we named bottlenose dolphin cov (bdcov) hku22. all the three bdcov hku22-positive samples were collected on the sa ... | 2014 | 24227844 |
| the avian coronavirus spike protein. | avian coronaviruses of the genus gammacoronavirus are represented by infectious bronchitis virus (ibv), the coronavirus of chicken. ibv causes a highly contagious disease affecting the respiratory tract and, depending on the strain, other tissues including the reproductive and urogenital tract. the control of ibv in the field is hampered by the many different strains circulating worldwide and the limited protection across strains due to serotype diversity. this diversity is believed to be due to ... | 2014 | 25451062 |
| the genotyping of infectious bronchitis virus in taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction. | infectious bronchitis virus (ibv; avian coronavirus) causes acute respiratory and reproductive and urogenital diseases in chickens. following sequence alignment of ibv strains, a combination of selective primer sets was designed to individually amplify the ibv wild-type and vaccine strains using a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction (arms rt-pcr) approach. this system was shown to discriminate the ibv wild-type and vaccine strains. m ... | 2014 | 25223342 |
| the endoplasmic reticulum stress sensor ire1α protects cells from apoptosis induced by the coronavirus infectious bronchitis virus. | the unfolded-protein response (upr) is a signal transduction cascade triggered by perturbation of the homeostasis of the endoplasmic reticulum (er). upr resolves er stress by activating a cascade of cellular responses, including the induction of molecular chaperones, translational attenuation, er-associated degradation, and other mechanisms. under prolonged and irremediable er stress, however, the upr can also trigger apoptosis. here, we report that in cells infected with the avian coronavirus i ... | 2014 | 25142592 |
| immunoglobulin a as an early humoral responder after mucosal avian coronavirus vaccination. | infectious bronchitis virus (ibv) is a highly contagious coronavirus prevalent in all countries with an extensive poultry industry and continues to cause economic losses. ibv strains of the ark serotype are highly prevalent in the southeastern united states despite extensive vaccination. one explanation for this observation is the high genetic variability of ibv. in addition, ibv ark-type vaccines may induce suboptimal mucosal immune responses, contributing to the prevalence and persistence of t ... | 2014 | 25055633 |
| coronavirus nsp6 restricts autophagosome expansion. | autophagy is a cellular response to starvation that generates autophagosomes to carry long-lived proteins and cellular organelles to lysosomes for degradation. activation of autophagy by viruses can provide an innate defense against infection, and for (+) strand rna viruses autophagosomes can facilitate assembly of replicase proteins. we demonstrated that nonstructural protein (nsp) 6 of the avian coronavirus, infectious bronchitis virus (ibv), generates autophagosomes from the er. a statistical ... | 2014 | 24991833 |
| differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus ibv strains qx and b1648 are not related to the sialic acid binding properties of their spike proteins. | the avian coronavirus (avcov) infectious bronchitis virus (ibv) is a major poultry pathogen. a characteristic feature of ibv is the occurrence of many different strains belonging to different serotypes, which makes a complete control of the disease by vaccinations a challenging task. reasons for differences in the tissue tropism and pathogenicity between ibv strains, e.g. a predilection for the kidneys or the oviduct are still an open question. strains of the qx genotype have been major pathogen ... | 2014 | 24928425 |
| sambucus nigra extracts inhibit infectious bronchitis virus at an early point during replication. | infectious bronchitis virus (ibv) is a pathogenic chicken coronavirus. currently, vaccination against ibv is only partially protective; therefore, better preventions and treatments are needed. plants produce antimicrobial secondary compounds, which may be a source for novel anti-viral drugs. non-cytotoxic, crude ethanol extracts of rhodiola rosea roots, nigella sativa seeds, and sambucus nigra fruit were tested for anti-ibv activity, since these safe, widely used plant tissues contain polyphenol ... | 2014 | 24433341 |
| evaluation of protection conferred by a vaccination program based on the h120 and cr88 commercial vaccines against a field variant of avian infectious bronchitis virus. | due to serotype variations among different avian infectious bronchitis viruses isolated in tunisia since 2000, protection of chicks, especially broiler flocks, with mass h120 vaccine often fails. therefore, association of cr88 (793b type) with h120 vaccines was used for better response. challenge experiments were then conducted to evaluate tracheal and renal cross-protection in chickens immunized via nasal and eye drops. conferred protection was measured by clinical signs and macroscopic lesions ... | 2014 | 32288459 |
| detection of 793/b serotype of infectious bronchitis virus in tissue sample by indirect immunoperoxidase assay. | the indirect immunoperoxidase (iip) assay was compared with the reverse transcription-polymerase chain reaction (rt-pcr) for detection of 793/b serotype of infectious bronchitis virus in tissues samples collected from experimentally infected chickens. this technique was optimized in specific pathogen-free (spf)-embryonated chicken eggs and broiler chickens inoculated with the iranian ir/773/2001 strain of 793/b serotype the trachea, lung, kidney, and cecal tonsil tissue samples from experimental ... | 2014 | 32214974 |
| a genome-wide association study identifies major loci affecting the immune response against infectious bronchitis virus in chicken. | coronaviruses are a hot research topic because they can cause severe diseases in humans and animals. infectious bronchitis virus (ibv), belonging to gamma-coronaviruses, causes a highly infectious respiratory viral disease and can result in catastrophic economic losses to the poultry industry worldwide. unfortunately, the genetic basis of the host immune responses against ibv is poorly understood. in the present study, the antibody levels against ibv post-immunization were measured by an enzyme- ... | 2014 | 24333371 |
| adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations. | mannose-binding lectin (mbl) plays a major role in the immune response as a soluble pattern-recognition receptor. mbl deficiency and susceptibility to different types of infections have been subject to extensive studies over the last decades. in humans and chickens, several studies have shown that mbl participates in the protection of hosts against virus infections. infectious bronchitis (ib) is a highly contagious disease of economic importance in the poultry industry caused by the coronavirus ... | 2014 | 24305086 |
| development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of s1 gene of h120 strain. | infectious bronchitis (ib), caused by infectious bronchitis virus (ibv), is a highly contagious chicken disease, and can lead to serious economic losses in poultry enterprises. the continual introduction of new ibv serotypes requires alternative strategies for the production of timely and safe vaccines against the emergence of variants. modification of the ibv genome using reverse genetics is one way to generate recombinant ibvs as the candidates of new ibv vaccines. in this study, the recombina ... | 2014 | 24287931 |
| complete genome sequence of a novel infectious bronchitis virus strain circulating in china with a distinct s gene. | an avian infectious bronchitis virus (ibv) was isolated and identified from a commercial layer flock vaccinated with live attenuated h120 vaccine in china, designed as ck/ch/ibtz/2012. to determine the origination and evolution of this isolated strain, we have carried out a complete genome sequencing of this strain. the genome of the ck/ch/ibtz/2012 strain is 27,691 nucleotides in length and includes more than 10 open reading frames. sequence comparison and phylogenetic analysis based on the ful ... | 2014 | 24682939 |
| pathogenicity and molecular characteristics of infectious bronchitis virus (ibv) strains isolated from broilers showing diarrhoea and respiratory disease. | abstract 1. the possibility that infectious bronchitis virus (ibv) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those ibv strains causing classical respiratory disease was investigated. 2. ibv variants were isolated from broiler flocks with enteric and respiratory problems in two regions of brazil. the usp-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into ibv-antibody-free broilers a ... | 2014 | 24678626 |
| assessment of the potential relationship between egg quality and infectious bronchitis virus infection in australian layer flocks. | this investigation aimed to determine if there was a relationship between the production of eggs with poor internal quality, as measured by poor haugh units, by australian layer flocks and the detection of infectious bronchitis virus (ibv) in the hens. other risk factors including flock size, flock type, flock age, chicken breed and vaccination frequency were also assessed. | 2014 | 24673141 |
| in this issue - april 2014: perceptions of 2007 equine influenza outbreak · hyperinsulinaemia in ponies · hydration and perennial ryegrass toxicosis · travel history needed for possible canine leishmaniosis · complications of arthroscopy · metaphyseal osteopathy in kelpies · infectious bronchitis virus and egg quality. | 2014 | 24673131 | |
| palmitoylation is required for intracellular trafficking of influenza b virus nb protein and efficient influenza b virus growth in vitro. | all influenza viruses bud and egress from lipid rafts within the apical plasma membrane of infected epithelial cells. as a result, all components of progeny virions must be transported to these lipid rafts for assembly and budding. although the mechanism of transport for other influenza proteins has been elucidated, influenza b virus (ibv) glycoprotein nb subcellular localization and transport are not understood completely. to address the aforementioned properties of nb, a series of trafficking ... | 2014 | 24671751 |
| origin and characteristics of the recombinant novel avian infectious bronchitis coronavirus isolate ck/ch/ljl/111054. | recombination among infectious bronchitis viruses (ibvs), coupled with point mutations, insertions, and deletions that occur in the genome, is thought to contribute to the emergence of new ibv variants. in this study an ibv, ck/ch/ljl/111054, was isolated from a h120-vaccinated chicken, which presented with a suspected ibv infection. phylogenetic analysis of the s1 subunit sequence confirmed that strain ck/ch/ljl/111054 is of the connecticut-type; however, further extensive full-length genomic a ... | 2014 | 24613433 |
| effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct. | the aim of this study was to determine the mechanism by which the avian infectious bronchitis virus (ibv) affects eggshell formation. attenuated ibv (aibv group) or vehicle (control group) was injected into the oviductal magnum lumen of white leghorn laying hens. the changes in the expression of genes related to eggshell formation (collagen types i and v, and cabp-d28k), densities of cytotoxic cells (cd8(+) and tcr-γδ(+) t cells), and gene expression of molecules related to cytotoxic immunoreact ... | 2014 | 24612789 |
| comparative proteome analysis of tracheal tissues in response to infectious bronchitis coronavirus, newcastle disease virus, and avian influenza virus h9 subtype virus infection. | infectious bronchitis coronavirus (ibv), newcastle disease virus (ndv), and avian influenza virus (aiv) h9 subtype are major pathogens of chickens causing serious respiratory tract disease and heavy economic losses. to better understand the replication features of these viruses in their target organs and molecular pathogenesis of these different viruses, comparative proteomic analysis was performed to investigate the proteome changes of primary target organ during ibv, ndv, and aiv h9 infections ... | 2014 | 24610701 |
| high-level protein expression following single and dual gene cloning of infectious bronchitis virus n and s genes using baculovirus systems. | baculovirus is an efficient system for the gene expression that can be used for gene transfer to both insect and different vertebrate hosts. the nucleocapsid gene (n) of the infectious bronchitis virus was cloned in a baculovirus expression system for insect cell expression. dual expression vectors containing ibv n and spike (s) proteins of the avian infectious bronchitis virus were engineered under the control of human and murine cytomegalovirus immediate-early enhancer/promoter elements in com ... | 2014 | 24605790 |
| induction of innate immune response following infectious bronchitis corona virus infection in the respiratory tract of chickens. | infectious bronchitis virus (ibv) replicates in the epithelial cells of trachea and lungs of chicken, however the mechanism of generation of innate immune response against ibv infection in these tissues has not been fully characterized. our objective was to study innate responses induced early following ibv infection in chickens. initiation of the transcription of selected innate immune genes such as tlr3, tlr7, myd88, il-1β and ifn-β, as well as recruitment of macrophages, were evident followin ... | 2014 | 24503073 |
| universal influenza b virus genomic amplification facilitates sequencing, diagnostics, and reverse genetics. | although human influenza b virus (ibv) is a significant human pathogen, its great genetic diversity has limited our ability to universally amplify the entire genome for subsequent sequencing or vaccine production. the generation of sequence data via next-generation approaches and the rapid cloning of viral genes are critical for basic research, diagnostics, antiviral drugs, and vaccines to combat ibv. to overcome the difficulty of amplifying the diverse and ever-changing ibv genome, we developed ... | 2014 | 24501036 |
| modeling inoculum dose dependent patterns of acute virus infections. | inoculum dose, i.e. the number of pathogens at the beginning of an infection, often affects key aspects of pathogen and immune response dynamics. these in turn determine clinically relevant outcomes, such as morbidity and mortality. despite the general recognition that inoculum dose is an important component of infection outcomes, we currently do not understand its impact in much detail. this study is intended to start filling this knowledge gap by analyzing inoculum dependent patterns of viral ... | 2014 | 24440713 |
| comparison of vaccine subpopulation selection, viral loads, vaccine virus persistence in trachea and cloaca, and mucosal antibody responses after vaccination with two different arkansas delmarva poultry industry -derived infectious bronchitis virus vaccines. | factors responsible for the persistence of arkansas delmarva poultry industry (arkdpi)-derived infectious bronchitis vaccines in commercial flocks and the high frequency of isolation of arkdpi-type infectious bronchitis viruses in respiratory cases are still unclear. we compared dynamics of vaccine viral subpopulations, viral loads, persistence in trachea and cloaca, and the magnitude of infectious bronchitis virus (1bv)-specific antibody induction after vaccination with two commercial arkdpi-de ... | 2014 | 24758121 |
| infectious bronchitis virus s2 expressed from recombinant virus confers broad protection against challenge. | we developed a recombinant newcastle disease virus (ndv) lasota (rls) expressing the infectious bronchitis virus (ibv) s2 gene (rls/ibv.s2). the recombinant virus showed somewhat-reduced pathogenicity compared to the parental lentogenic lasota strain but effectively elicited hemagglutination inhibition antibodies against ndv and protected chickens against lethal challenge with virulent ndv/ca02. ibv heterotypic protection was assessed using a prime-boost approach with a commercially available at ... | 2014 | 24758118 |
| genotyping of infectious bronchitis viruses identified in canada between 2000 and 2013. | infectious bronchitis virus (ibv) was detected in 185 samples originating from chicken flocks of various commodity groups in canada. flocks with clinical signs such as respiratory challenge, sudden death, egg production problems, or nephropathogenic conditions, and randomly selected flocks sampled at slaughter as part of an ontario broiler surveillance project, were included. most samples were from ontario and québec; however, a small number from british columbia, nova scotia, and newfoundland a ... | 2014 | 24754612 |
| evaluation of a novel strain of infectious bronchitis virus emerged as a result of spike gene recombination between two highly diverged parent strains. | the emergence of new variant strains of the poultry pathogen infectious bronchitis virus (ibv) is continually reported worldwide, owing to the labile nature of the large single-stranded rna ibv genome. high resolution melt curve analysis previously detected a variant strain, n1/08, and the present study confirmed that this strain had emerged as a result of recombination between australian subgroup 2 and 3 strains in the spike gene region, in a similar manner reported for turkey coronaviruses. th ... | 2014 | 24730363 |
| dramatic differences in the response of macrophages from b2 and b19 mhc-defined haplotypes to interferon gamma and polyinosinic:polycytidylic acid stimulation. | the chicken mhc has been associated with disease resistance, though the mechanisms are not understood. the functions of macrophages, critical to both innate and acquired immunity, were compared between the more infectious bronchitis virus-resistant b2 and the more infectious bronchitis virus-susceptible b19 lines. in vivo peripheral blood concentrations of monocytes were similar in b2 or b19 homozygous haplotypes. peripheral blood-derived macrophages were stimulated with poly i:c, simulating an ... | 2014 | 24706959 |
| infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes. | replication of positive-sense rna viruses is associated with the rearrangement of cellular membranes. previous work on the infection of tissue culture cell lines with the betacoronaviruses mouse hepatitis virus and severe acute respiratory syndrome coronavirus (sars-cov) showed that they generate double-membrane vesicles (dmvs) and convoluted membranes as part of a reticular membrane network. here we describe a detailed study of the membrane rearrangements induced by the avian gammacoronavirus i ... | 2013 | 24149513 |
| upregulation of chop/gadd153 during coronavirus infectious bronchitis virus infection modulates apoptosis by restricting activation of the extracellular signal-regulated kinase pathway. | induction of the unfolded protein response (upr) is an adaptive cellular response to endoplasmic reticulum (er) stress that allows a cell to reestablish er homeostasis. however, under severe and persistent er stress, prolonged upr may activate unique pathways that lead to cell death. in this study, we investigated the activation of the protein kinase r-like er kinase (perk) pathway of upr in cells infected with the coronavirus infectious bronchitis virus (ibv) and its relationship with ibv-induc ... | 2013 | 23678184 |
| suppression of coronavirus replication by cyclophilin inhibitors. | coronaviruses infect a variety of mammalian and avian species and cause serious diseases in humans, cats, mice, and birds in the form of severe acute respiratory syndrome (sars), feline infectious peritonitis (fip), mouse hepatitis, and avian infectious bronchitis, respectively. no effective vaccine or treatment has been developed for sars-coronavirus or fip virus, both of which cause lethal diseases. it has been reported that a cyclophilin inhibitor, cyclosporin a (csa), could inhibit the repli ... | 2013 | 23698397 |
| the cellular interactome of the coronavirus infectious bronchitis virus nucleocapsid protein and functional implications for virus biology. | the coronavirus nucleocapsid (n) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. these functions are likely to be facilitated by interactions with host cell proteins. the potential interactome of the infectious bronchitis virus (ibv) n protein was mapped using stable isotope labeling with amino acids in cell culture (silac) coupled to a green fluorescent protein-nanotrap pull ... | 2013 | 23637410 |
| visualizing the autophagy pathway in avian cells and its application to studying infectious bronchitis virus. | autophagy is a highly conserved cellular response to starvation that leads to the degradation of organelles and long-lived proteins in lysosomes and is important for cellular homeostasis, tissue development and as a defense against aggregated proteins, damaged organelles and infectious agents. although autophagy has been studied in many animal species, reagents to study autophagy in avian systems are lacking. microtubule-associated protein 1 light chain 3 (map1lc3/lc3) is an important marker for ... | 2013 | 23328491 |
| how the double spherules of infectious bronchitis virus impact our understanding of rna virus replicative organelles. | powered by advances in electron tomography, recent studies have extended our understanding of how viruses construct "replication factories" inside infected cells. their function, however, remains an area of speculation with important implications for human health. it is clear from these studies that whatever their purpose, organelle structure is dynamic (m. ulasli, m. h. verheije, c. a. de haan, and f. reggiori, cell. microbiol. 12:844-861, 2010) and intricate (k. knoops, m. kikkert, s. h. worm, ... | 2013 | 24345746 |
| assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus m and s proteins. | infectious bronchitis virus (ibv) as an avian coronavirus is still posing a persistent and imminent threat to the poultry industry worldwide. here we report that transfection of sf9 cells with a single recombinant baculovirus encoding m and s proteins resulted in the assembly of ibv vlps; this is the first report that s protein plus m protein alone were able to be assembled into vlps for coronaviruses. we further showed that the generated ibv vlps could induce humoral immune responses in a level ... | 2013 | 24099870 |
| contributions of the s2 spike ectodomain to attachment and host range of infectious bronchitis virus. | the spike protein is the major viral attachment protein of the avian coronavirus infectious bronchitis virus (ibv) and ultimately determines viral tropism. the s1 subunit of the spike is assumed to be required for virus attachment. however, we have previously shown that this domain of the embryo- and cell culture adapted beaudette strain, in contrast to that of the virulent m41 strain, is not sufficient for binding to chicken trachea (wickramasinghe et al., 2011). in the present study, we demons ... | 2013 | 24041648 |
| identification and survey of a novel avian coronavirus in ducks. | the rapid discovery of novel viruses using next generation sequencing (ngs) technologies including dna-seq and rna-seq, has greatly expanded our understanding of viral diversity in recent years. the timely identification of novel viruses using ngs technologies is also important for us to control emerging infectious diseases caused by novel viruses. in this study, we identified a novel duck coronavirus (cov), distinct with chicken infectious bronchitis virus (ibv), using rna-seq. the novel duck-s ... | 2013 | 24023656 |