Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| hepatitis b surface antigen promotes the invasion of hepatitis b virus-related hepatocellular carcinoma cells by upregulation of toll-like receptor 2. | hepatitis b virus (hbv) infection is one of the major risk factors leading to the development of hepatocellular carcinoma (hcc). hepatitis b surface antigen (hbsag) plays a pivotal role in hbv-related hcc pathogenesis, and toll-like receptor (tlr) 2 is also considered to mediate tumor progression. however, the interaction between hbsag and tlr2 in hcc progression remains unclear. thus, the aim of the study was to explore the effect of hbsag-tlr2 pathway on growth and invasion of hbv-related hcc ... | 2017 | 28061318 |
| serum microrna profiles as diagnostic biomarkers for hbv-positive hepatocellular carcinoma. | the discovery of effective and reliable biomarkers to detect hepatitis b virus (hbv)-positive hepatocellular carcinoma (hcc) at an early stage may improve the survival of hcc. the aim of this study was to establish serum microrna (mirna) profiles as diagnostic biomarkers for hbv-positive hcc. | 2017 | 28061012 |
| estimating the cost-effectiveness of one-time screening and treatment for hepatitis c in korea. | this study aims to investigate the cost-effectiveness of a one-time hepatitis c virus (hcv) screening and treatment program in south korea where hepatitis b virus (hbv) prevails, in people aged 40-70, compared to current practice (no screening). | 2017 | 28060834 |
| influences of obesity on the immunogenicity of hepatitis b vaccine. | hepatitis b vaccine is regarded as the most effective method for the prevention of hepatitis b virus (hbv) infection. however, several factors such as age, body mass index and immunocompetent state have been reported to be associated with reduced immunization responses. the present commentary was aimed to discuss the influences of obesity on the immunogenicity of hepatitis b vaccines. | 2017 | 28059607 |
| prognosis of intrahepatic cholangiocarcinomas with hbv infection is better than those with hepatolithiasis after r0 liver resection: a propensity score matching analysis. | the impact of different causative factors of intrahepatic cholangiocarcinoma (icc) on disease outcome remains largely unknown. this study aimed to evaluate the prognosis of icc patients with different pathogenic factors after hepatectomy. | 2017 | 28058554 |
| severe hepatitis b virus reactivation related to ibrutinib monotherapy. | 2017 | 28058492 | |
| hepatitis b virus strains from rwandan blood donors are genetically similar and form one clade within subgenotype a1. | rwanda is a central african country with about 12 million inhabitants. the 1994 genocide against the tutsi destroyed much of the infrastructure, including the health system. although this has improved significantly, many challenges remain to be addressed. in this study, the prevalence of serological markers of past and ongoing hepatitis b virus (hbv) infection and hbv vaccine related immunity was investigated in samples from blood donors from all regions of rwanda. | 2017 | 28056881 |
| platelet counts modulate the quantitative relationship between hepatitis b viral dna and surface antigen concentrations: a cross-sectional study of hematological, histological and viral factors. | the concentrations of hepatitis b virus (hbv) dna and surface antigen (hbsag) are two critical virological variables to be monitored in chronic hepatitis b. hbsag is derived from the hbv genome. thus, higher hbv-dna concentrations should implicate higher hbsag levels. nevertheless, the two variables do not manifest a simple linear relationship due to elusive host factor involvements. the aim of this study was to address the discrepancy of hbv dna and hbsag levels by a quantitative modeling of hb ... | 2017 | 28056849 |
| inhibiting the secretion of hepatitis b surface antigen (hbsag) to treat hepatitis b infection- a review. | chronic hepatitis b is a major global health issue, and has no known cure. currently there are over 240 million people infected with the disease, and it leads to the death of over 686,000 people each year. a total of seven treatments which help to control the disease are currently available: interferon-based treatments (pegasys, and interferon alpha), and nucleoside and nucleotide analogs (viread, baraclude, tyzeka, hepsera, epivir-hbv), but all require continuing treatment to maintain control o ... | 2017 | 28056752 |
| engineering hepatitis b virus core particles for targeting her2 receptors in vitro and in vivo. | hepatitis b virus core (hbc) particles have been studied for their potential as drug delivery vehicles for cancer therapy. hbc particles are hollow nano-particles of 30-34 nm diameter and 7 nm thick envelopes, consisting of 180-240 units of 21 kda core monomers. they have the capacity to assemble/dis-assemble in a controlled manner allowing encapsulation of various drugs and other biomolecules. moreover, other functional motifs, i.e. receptors, receptor binding sequences, peptides and proteins c ... | 2017 | 28056402 |
| mita/sting and its alternative splicing isoform mrp restrict hepatitis b virus replication. | an efficient clearance of hepatitis b virus (hbv) requires the coordinated work of both the innate and adaptive immune responses. mita/sting, an adapter protein of the innate immune signaling pathways, plays a key role in regulating innate and adaptive immune responses to dna virus infection. previously, we identified an alternatively spliced isoform of mita/sting, called mita-related protein (mrp), and found that mrp could specifically block mita-mediated interferon (ifn) induction while retain ... | 2017 | 28056087 |
| persistent risk for hepatocellular carcinoma after more than a decade of successful hepatitis b virus suppression. | 2017 | 28054479 | |
| hbx promotes cell proliferation by disturbing the cross-talk between mir-181a and pten. | hepatitis b virus x protein (hbx) is involved in the initiation and progression of hepatocellular carcinoma (hcc). however, the mechanism is still needed to be elucidated. in this study, we explored the relationship between hbx and microrna and their roles in hepato-carcinogenesis. firstly, by global microarray-based microrna profiling and qrt-pcr, we found mir-181a was strongly up-regulated in hepg2.2.15 cells (hbv positive) and phbv1.3-expressing hepg2 cells, and hbx played a major role in it. ... | 2017 | 28053323 |
| treatment of chronic hepatitis b infection-2017. | since the registration of the first effective nucleoside analogue against the hepatitis b virus almost two decades ago, major progress has been made in the management of chronic hepatitis b infection. however, hepatitis b-related morbidity and mortality remain a major global health threat. this is partly due to the escalating costs and the decrease in compliance related to the need for prolonged therapy for most patients who cannot be "cured". new biomarkers such as quantitative hepatitis b surf ... | 2017 | 28052634 |
| challenges in warranting access to prophylaxis and therapy for hepatitis b virus infection. | despite an available vaccine and efficient treatment for hepatitis b virus (hbv) infection, chronic hbv infection still remains a major global threat, and one of the top 20 causes of human mortality worldwide. one of the major challenges in controlling hbv infection is the high number of undiagnosed chronic carriers and the lack of access to prophylaxis and treatment in several parts of the world. we discuss relevant barriers that need to be overcome to achieve global control of hbv infection an ... | 2017 | 28052625 |
| novel targets for hepatitis b virus therapy. | treatment with either pegylated interferon-alpha (pegifn-α) or last generation nucleos(t)ide analogues (nas) successfully leads to serum viral load suppression in most chronically infected hepatitis b (chb) patients, but hbsag loss is only achieved in 10% of the cases after a 5-year follow-up. thus, therapy must be administered long-term and it will not completely eliminate infection because of the persistent hepatitis b virus (hbv) minichromosome in infected cells, and cannot completely abolish ... | 2017 | 28052622 |
| hepatitis b virus long-term impact of antiviral therapy nucleot(s)ide analogues (nucs). | the goal of antiviral therapy is to improve the quality of life and survival of patients with chronic hepatitis b (chb) by halting the progression to cirrhosis, end-stage liver disease or hepatocellular carcinoma (hcc), thus preventing anticipated liver-related death. oral administration of potent and less resistance-prone nucleot(s)ide analogues (nucs), such as entecavir (etv) and tenofovir disoproxil fumarate (tdf) has become the most popular treatment strategy worldwide because of their excel ... | 2017 | 28052621 |
| the impact of direct antiviral agents on the development and recurrence of hepatocellular carcinoma. | direct antiviral agents mark a major progress for the treatment of chronic hepatitis c virus infection. the rate of cure is higher than 90% in most populations and the safety profile is good. however, like any treatment, there are potential unexpected adverse events. several reports have indicated that antiviral therapy may be associated with the reactivation of hepatitis b virus or the emergence of herpes virus in a time-related manner. recently, several studies have described a potential unexp ... | 2017 | 28052619 |
| the future of liver transplantation for viral hepatitis. | in hepatitis c virus (hcv)-infected patients, transplantation can be justified by decompensated cirrhosis, hepatocellular carcinoma (hcc) or both. during the last decade, hcv infection accounted for about 30% of the indications for transplantation in europe and north america. direct antiviral agents (daas) are highly effective at curing hcv, even in patients with end-stage cirrhosis. in the future, the incidence of hcv-related decompensated cirrhosis will continue to decrease. the incidence of h ... | 2017 | 28052618 |
| effects of interleukin-4 and interleukin-12b gene polymorphisms on hepatitis b virus vaccination. | approximately 10% of individuals do not respond to hepatitis b virus (hbv) vaccination, i.e. non-responders (nrs). we aimed to investigate the association of interleukin (il)-4 and il-12b gene polymorphisms with responsiveness to the hbv vaccine in korean infants. among 300 healthy infants (9-12 month), snps for the il-4 gene (rs2243250, rs2070874, and rs2227284) and for the il-12b gene (rs3213094 and rs17860508) were compared between subgroups in terms of the response to hbv vaccination. the pe ... | 2017 | 28051794 |
| association between hepatitis b virus and chronic kidney disease: a systematic review and meta-analysis. | background. hepatitis b virus infection and chronic kidney disease are prevalent and remain a major public health problem worldwide. it remains unclear how infection with hepatitis b virus impacts on the development and progression of chronic kidney disease. | 2017 | 28051791 |
| impact of viral etiology on postoperative de novo recurrence after hepatectomy for hepatocellular carcinoma in cirrhotic patients. | liver cirrhosis (lc) and hepatocellular carcinoma (hcc) are associated with viral hepatitis, especially hepatitis b virus (hbv) and hepatitis c virus (hcv). whether differences exist in postoperative de novo carcinogenesis from established cirrhosis according to viral etiology remains unclear. | 2017 | 28050767 |
| do recommendations by healthcare providers, family-members, friends, and individual self-efficacy increase uptake of hepatitis b screening? results of a population-based study of asian americans. | hepatitis b virus (hbv) infection disproportionately affects asian americans but hbv screening rates among asian american are substantially low. this study examines the impact of multiple recommendations and self-efficacy on hbv screening uptake among asian americans. | 2017 | 28058203 |
| late reactivation of hepatitis b virus after chemotherapies for hematological malignancies: a case report and review of the literature. | reactivation of hepatitis b virus (hbv) is a serious complication of immunosuppressive therapy and cytotoxic chemotherapy. the optimal duration of hbv-dna monitoring for at-risk patients depends on the clinical features of reactivation, especially the range of potency from therapies to reactivation. we present a case of very late reactivation after chemotherapy for lymphoma and review previous reports of late reactivation cases. we also underscore the significance of developing an indicator for ... | 2017 | 28049989 |
| investigation of a novel hepatitis b virus surface antigen (hbsag) escape mutant affecting immunogenicity. | mutation in the hepatitis b virus surface antigen (hbsag) may affect the efficiency of diagnostic immunoassays or success of vaccinations using hbsag. thus, antigenicity and immunogenicity analyses of the mutated hbsag are necessary to develop novel diagnostic tools and efficient vaccinations. here, the in vitro antigenicity of three wild-type hbsag open reading frames (orfs) (adr4, w1s [subtype adr] and w3s [subtype adr]) isolated from clinically infected patients and nineteen synthesized singl ... | 2017 | 28045894 |
| impact of viral status on survival in patients receiving sorafenib for advanced hepatocellular cancer: a meta-analysis of randomized phase iii trials. | purpose following the sorafenib hepatocellular carcinoma assessment randomized protocol (sharp) trial, sorafenib has become the standard of care for patients with advanced unresectable hepatocellular carcinoma, but the relation between survival advantage and disease etiology remains unclear. to address this, we undertook an individual patient data meta-analysis of three large prospective randomized trials in which sorafenib was the control arm. methods of a total of 3,256 patients, 1,643 (50%) w ... | 2017 | 28045619 |
| transforming growth factor β-activated kinase 1 transcriptionally suppresses hepatitis b virus replication. | hepatitis b virus (hbv) replication in hepatocytes is restricted by the host innate immune system and related intracellular signaling pathways. transforming growth factor β-activated kinase 1 (tak1) is a key mediator of toll-like receptors and pro-inflammatory cytokine signaling pathways. here, we report that silencing or inhibition of endogenous tak1 in hepatoma cell lines leads to an upregulation of hbv replication, transcription, and antigen expression. in contrast, overexpression of tak1 sig ... | 2017 | 28045080 |
| identification of amino acids in antigen-binding site of class ii hla proteins independently associated with hepatitis b vaccine response. | genetic factors in class ii human leukocyte antigen (hla) have been reported to be associated with inter-individual variation in hepatitis b virus (hbv) vaccine response. however, the mechanism underlying the associations remains elusive. in particular, the broad linkage disequilibrium in hla region complicates the localization of the independent effects of genetic variants. thus, the present study aimed to identify the most probable causal variations in class ii hla loci involved in the immune ... | 2017 | 28043736 |
| occurrence of needlestick and injuries among health-care workers of a tertiary care teaching hospital in north india. | occupational hazards such as accidental exposure to sharp, cuts, and splashes are common among health-care workers (hcws). | 2017 | 28042212 |
| role of interleukin-23 in monocyte-derived dendritic cells of hbv-related acute-on-chronic liver failure and its correlation with the severity of liver damage. | the role of interleukin-23 (il-23) in monocyte-derived dendritic cells (modcs) from hepatitis b virus-related acute-on-chronic liver failure (hbv-aclf) patients remains unclear. the aim of this study was to observe the correlation between the activation of the il-23 signaling pathway and the prognosis of hbv-aclf patients. | 2017 | 28041935 |
| the hla-g 14-base pair deletion allele and the deletion/deletion genotype are associated with persistent hbe antigenemia in chronic hepatis b infection. | hla-g has well-recognized immunomodulatory properties, and this molecule is frequently expressed in the livers of hepatitis b virus (hbv)-infected patients. because the hla-g 14 bp-insertion/deletion polymorphism (rs371194629) has been associated with the magnitude of hla-g expression, we evaluated this polymorphism in the recognized evolutionary forms of chronic hbv infection. | 2017 | 28041834 |
| s-1 plus sorafenib for the treatment of advanced hepatocellular carcinoma. | to assess the efficacy and safety of s-1 plus sorafenib for the treatment of advanced hepatocellular carcinoma (hcc). | 2017 | 28039697 |
| hepatitis b virus infection and decreased risk of nonalcoholic fatty liver disease: a cohort study. | the presence of an association between chronic hepatitis b virus (hbv) infection and fatty liver is controversial. we examined the association between hbv infection and the development of nonalcoholic fatty liver disease (nafld). we conducted a cohort study of 83,339 participants without nafld at baseline who underwent serologic testing for hepatitis b surface antigen (hbsag) between 2002 and 2006 and were followed annually or biennially until december 2014. nafld was defined as the presence of ... | 2017 | 28035771 |
| rapid alanine aminotransferase normalization with entecavir and hepatocellular carcinoma in hepatitis b virus-associated cirrhosis. | sustained abnormal serum alanine aminotransferase (alt) levels can increase the risk of hepatocellular carcinoma (hcc) in patients with chronic hepatitis b. | 2017 | 28035553 |
| variations in il-1r1 gene influence risk for hepatitis b virus infection of children in a han chinese population. | host genetic factors play an important role in the pathogenesis of hepatitis b virus (hbv) infection. however, the role of interleukin1receptor, type i (il-1r1) gene in hbv infection and breakthrough infection in children remains unclear. the aim of this study was to investigate the association between snps in il-1 family and hbv infection and breakthrough infection in children. | 2017 | 28027994 |
| hbv inhibits lps-induced nlrp3 inflammasome activation and il-1β production via suppressing the nf-κb pathway and ros production. | hepatitis b virus (hbv) has developed strategies to evade immune responses. however, the mechanisms involved remain unclear. the nlrp3 inflammasome plays crucial roles in antiviral host defense and its downstream factor il-1β has been shown to inhibit hbv infection in vivo. this study aims to assess whether hbv can affect the nlrp3 inflammasome signaling pathways and shed light on the underlying mechanisms hbv utilizes to evade host innate immune responses. | 2017 | 28027970 |
| longterm prognosis of combined hepatocellular carcinoma-cholangiocarcinoma following liver transplantation and resection. | combined hepatocellular carcinoma-cholangiocarcinoma (chcc-cc) is a rare disease. we investigated the clinicopathological features of chcc-cc and compared the longterm outcomes following liver transplantation (lt) and hepatic resection (hr). we identified 32 lt patients with chcc-cc through an institutional database search. the hr control group (n = 100) was selected through propensity score-matching. the incidence of chcc-cc among all adult lt patients was 1.0%. mean patient age was 53.4 ± 6.7 ... | 2017 | 28027599 |
| hepatitis b reactivation in hbsag-negative/hbcab-positive patients receiving immunosuppressive therapy for glomerulonephritis: a retrospective analysis. | the aim of this study is to investigate the prevalence and risk factors for hepatitis b virus (hbv) reactivation in hbsag-negative/hbcab-positive patients receiving immunosuppressive therapy for glomerulonephritis. | 2017 | 28032257 |
| t cells infiltrating diseased liver express ligands for the nkg2d stress surveillance system. | nk cells, which are highly enriched in the liver, are potent regulators of antiviral t cells and immunopathology in persistent viral infection. we investigated the role of the nkg2d axis in t cell/nk cell interactions in hepatitis b. activated and hepatitis b virus (hbv)-specific t cells, particularly the cd4 fraction, expressed nkg2d ligands (nkg2dl), which were not found on t cells from healthy controls (p < 0.001). nkg2dl-expressing t cells were strikingly enriched within hbv-infected livers ... | 2017 | 28031333 |
| subtypes and risk behaviors among incident hiv cases in the bangkok men who have sex with men cohort study, thailand, 2006-2014. | hiv-1 incidence and prevalence remain high among men who have sex with men (msm), and transgender women (tgw), in thailand. to examine the link between epidemiologic factors and hiv-1 subtype transmission among thai msm, we compared covariates of infection with hiv crf01_ae and other hiv strains among participants in the bangkok msm cohort study (bmcs). the bmcs was an observational cohort study of thai msm and tgw with up to 60 months of follow-up at 4 monthly intervals. participants underwent ... | 2017 | 28019101 |
| analysis of the prevalence of drug-resistant hepatitis b virus in patients with antiviral therapy failure in a chinese tertiary referral liver centre (2010-2014). | to study the prevalence of drug-resistant hbv in patients with therapy failure in a chinese tertiary referral liver centre. | 2017 | 28017671 |
| risk factors related to the failure of prevention of hepatitis b virus mother-to-child transmission in yunnan, china. | to investigate the relationship between the failure of prevention of hepatitis b virus mother-to-child transmission and hbv serological pattern, viral load as well as hbv genotypes. | 2017 | 28017429 |
| reduction in liver transplant wait-listing in the era of direct-acting antiviral therapy. | direct-acting antiviral (daa) therapy, recently approved for patients with decompensated cirrhosis (dc) secondary to hepatitis c virus (hcv), is associated with improved hepatic function. we analyzed trends in liver transplant (lt) wait-listing (wl) to explore potential impact of effective medical therapy on wl registration. this is a cohort study using the scientific registry of transplant recipients database from 2003 to 2015. a total of 47,591 adults wait-listed for lt from hcv, hepatitis b v ... | 2017 | 28012259 |
| the history and challenges of blood donor screening in china. | since the establishment of people's republic of china in 1949, the chinese government has encountered several catastrophes related to transfusion transmitted diseases. the government's increasing attention to blood safety has prompted the initiation of a series of policies and measures that have enhanced the level of safety for the blood supply and met the basic clinical demands of blood for 1.3 billion people in the country. blood donation screening strategies in china predominantly comprise do ... | 2017 | 28012709 |
| predicting the clinical outcome of icsi by sperm head vacuole examination. | to assess whether high magnification sperm head vacuole examination (shve) and/or standard sperm morphology assessment can predict icsi outcomes in terms of fertilization, embryo quality, and delivery rates, a prospective observational bicentric study was conducted in two publicly funded assisted reproductive technology (art) units in france between january and july of 2012. a total of 111 icsi cycles for exclusively male infertility factors were included. a spearman's correlation test was perfo ... | 2017 | 28010131 |
| preoperatively staging liver fibrosis using noninvasive method in hepatitis b virus-infected hepatocellular carcinoma patients. | advanced liver fibrosis can result in serious complications (even patient's death) after partial hepatectomy. preoperatively percutaneous liver biopsy is an invasive and expensive method to assess liver fibrosis. we aim to establish a noninvasive model, on the basis of preoperative biomarkers, to predict liver fibrosis in hepatocellular carcinoma (hcc) patients with hepatitis b virus (hbv) infection. | 2017 | 28008144 |
| characteristics and course of chronic hepatitis b e antigen-negative infection. | to describe the epidemiological, analytical and histological characteristics and clinical course of hepatitis b virus (hbv) carriers with negative hbe antigen. | 2017 | 28007350 |
| recapitulation of treatment response patterns in a novel humanized mouse model for chronic hepatitis b virus infection. | there are ~350 million chronic carriers of hepatitis b (hbv). while a prophylactic vaccine and drug regimens to suppress viremia are available, chronic hbv infection is rarely cured. hbv's limited host tropism leads to a scarcity of susceptible small animal models and is a hurdle to developing curative therapies. mice that support engraftment with human hepatoctyes have traditionally been generated through crosses of murine liver injury models to immunodeficient backgrounds. here, we describe th ... | 2017 | 28006671 |
| response to tenofovir among lamivudine-experienced hepatitis b and hiv-coinfected adolescents. | we determined hepatitis b virus (hbv) suppression rates among 18 lamivudine (3tc)-experienced hbv-human immunodeficiency virus-coinfected adolescents after treatment with tenofovir disoproxil fumurate (tdf). at tdf initiation, their median age was 17.6 years, and duration of 3tc exposure was 7.3 years. eleven patients (61%) achieved hbv dna <60 iu/ml after 48 weeks on tdf and 3tc which was similar to adult studies, although hepatitis b surface antigen loss or hepatitis b envelope antigen serocon ... | 2017 | 28005687 |
| clinical significance of dynamics of programmed death ligand-1 expression on circulating cd14(+) monocytes and cd19(+) b cells with the progression of hepatitis b virus infection. | programmed death-1 (pd-1) expression has been revealed to be upregulated on t cells and contributes to t cell exhaustion in patients with hepatitis b virus (hbv) infection. in this study, we investigated the dynamic expression of programmed death ligand-1 (pd-l1), the ligand of pd-1, on circulating cd14(+) monocytes and cd19(+) b cells of hbv-infected patients at the stages of chronic hbv (chb) infection, liver cirrhosis (lc), and hepatocellular carcinoma (hcc), respectively. the results showed ... | 2017 | 28005469 |
| a follow-up study on the renal protective efficacy of telbivudine for hepatitis b virus-infected taiwanese patients after living donor liver transplant. | preservation of renal function is an important issue after living donor liver transplant. we aimed to examine the renal protective efficacy of telbivudine in hepatitis b virus-infected patients after living donor liver transplant. | 2017 | 28004999 |
| seroprevalence and diagnosis of hiv, hbv, hcv and syphilis infections among blood donors. | blood transfusion is one of the most important therapeutic options of life-saving intervention for recipients who are in diseased or non-diseased conditions with severe blood loss. however, it is associated with certain risks which can lead to adverse consequences that may cause acute or delayed complications and bring the risk of transfusion-transmissible infections including hiv, hepatitis b & c and syphilis. so, there might be a fatal risk instead of life saving. this paper aims to provide a ... | 2017 | 28009328 |
| assessment of semi-automated nucleic acid testing programme in a regional blood transfusion centre. | detection of human immunodeficiency virus type-1 (hiv-1), hepatitis-c (hcv) and hepatitis-b virus (hbv) in the blood donors is crucial. an efficient form of detection is nucleic acid testing (nat) in blood screening. we assessed the suitability of commercial nat testing in a developing country, focusing on the altona realstar assay and the method of sacace biotechnologies. | 2017 | 27996693 |
| measurement of antiviral effect and innate immune response during treatment of primary woodchuck hepatocytes. | an estimated 350 million people are chronically infected with hepatitis b virus (hbv), and over one million people die each year due to hbv-associated liver diseases, such as cirrhosis and liver cancer. current therapeutics for chronic hbv infection are limited to nucleos(t)ide analogs and interferon. these anti-hbv drugs in general reduce viral load and improve the long-term outcome of infection but very rarely lead to a cure. thus, new therapies for chronic hbv infection need to be developed b ... | 2017 | 27975326 |
| partial hepatectomy and castration of hbv transgenic mice. | hepatitis b virus (hbv) is a hepatotropic virus. its infection can cause liver injury and regeneration, and its replication is affected by the gender. transgenic mice that carry a 1.3-mer overlength hbv dna genome productively replicate hbv in hepatocytes and have been very useful for studying the replication and pathogenesis of hbv in vivo. by using this mouse model, the relationship between hbv and liver injury and regeneration as well as the effect of the gender on hbv gene expression and rep ... | 2017 | 27975324 |
| generation of replication-competent hepatitis b virus genome from blood samples for functional characterization. | hepatitis b virus (hbv) infection can be associated with a spectrum of clinical outcomes. transient transfection of the clinical hbv isolates in human hepatoma cell lines can establish their biological properties to shed light on their different pathogenic potentials, yet very few clinical hbv isolates have been functionally characterized so far. the technical challenges include faithful amplification of the full-length hbv genome from clinical samples and conversion into a replication-competent ... | 2017 | 27975320 |
| deep sequencing of the hepatitis b virus genome: analysis of multiple samples by implementation of the illumina platform. | the quasispecies variation of hepatitis b virus (hbv) was believed to be a viral response to antiviral treatment and host immune pressure. sanger sequencing was previously the classic approach for quasispecies analysis, but this method was also time-consuming and laborious. ultra-deep sequencing has been widely used in viral quasispecies research, especially for low-frequency mutation detection. here we present a multiple samples deep sequencing method employing the illumina platform to detect h ... | 2017 | 27975319 |
| detection of hepatitis b virus particles released from cultured cells by particle gel assay. | the culture fluid of hbv replicating cells contains a mixture of viral particles with different structural and genetic components, including enveloped infectious virions, genome-free virion, envelope-only subviral particles, and nonenveloped naked capsids. based on their different physical and chemical properties, the enveloped and nonenveloped particles can be separated by the native agarose gel electrophoresis and transferred onto a positively charged microporous membrane, then the virus parti ... | 2017 | 27975317 |
| in vitro enzymatic and cell culture-based assays for measuring activity of hbv rnaseh inhibitors. | hbv is a small, enveloped dna virus that replicates by reverse transcription via an rna intermediate. current anti-hbv treatment regiments that include interferon α and nucleos(t)ide analogs have insufficient efficiency, are of long duration and can be accompanied by systemic side effects. though hbv rnaseh is essential for viral replication, it is unexploited as a drug target against hbv. rnaseh inhibitors that actively block viral replication would represent an important addition to the potent ... | 2017 | 27975316 |
| in vitro assays for rna binding and protein priming of hepatitis b virus polymerase. | the hepatitis b virus (hbv) polymerase synthesizes the viral dna genome from the pre-genomic rna (pgrna) template through reverse transcription. initiation of viral dna synthesis is accomplished via a novel protein priming mechanism, so named because the polymerase itself acts as a primer, whereby the initiating nucleotide becomes covalently linked to a tyrosine residue on the viral polymerase. protein priming, in turn, depends on specific recognition of the packaging signal on pgrna called epsi ... | 2017 | 27975315 |
| measuring changes in cytosolic calcium levels in hbv- and hbx-expressing cultured primary hepatocytes. | chronic infection with hepatitis b virus (hbv) remains a major worldwide health concern and is the leading cause of hepatocellular carcinoma (hcc). the hbv x protein (hbx) is the only regulatory protein encoded in the hbv genome; hbx stimulates hbv replication in vivo and in vitro. hbx also regulates cytosolic ca(2+) signaling, and altered ca(2+) signaling is associated with the development of many diseases, including hcc. importantly, many hbx functions, including hbx modulation of cell prolife ... | 2017 | 27975314 |
| immunofluorescent staining for the detection of the hepatitis b core antigen in frozen liver sections of human liver chimeric mice. | the hepatitis b virus (hbv) is the causative agent for chronic hepatitis b infection, which affects an estimate of 240 million people worldwide and puts them at risk of developing terminal liver disease. the life cycle of the virus and its interactions with the host immune system are still incompletely understood, and currently available treatment options rarely achieve a cure. therefore, basic research and new drug development are needed. one parameter for measuring the intrahepatic activity of ... | 2017 | 27975313 |
| detection of hepatocyte clones containing integrated hepatitis b virus dna using inverse nested pcr. | chronic hepatitis b virus (hbv) infection is a major cause of liver cirrhosis and hepatocellular carcinoma (hcc), leading to ~600,000 deaths per year worldwide. many of the steps that occur during progression from the normal liver to cirrhosis and/or hcc are unknown. integration of hbv dna into random sites in the host cell genome occurs as a by-product of the hbv replication cycle and forms a unique junction between virus and cellular dna. analyses of integrated hbv dna have revealed that hccs ... | 2017 | 27975311 |
| analyses of hbv cccdna quantification and modification. | covalently closed circular dna (cccdna) serves as the transcriptional template of hepatitis b virus (hbv) replication in the nucleus of infected cells. it ensures the persistence of hbv even if replication is blocked. immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccdna can induce the loss of cccdna. for studies on hbv control, the analysis of cccdna integrity and its exact quantification is very important. here, we describe diff ... | 2017 | 27975308 |
| a homokaryon assay for nucleocytoplasmic shuttling activity of hbv core protein. | hepatitis b virus (hbv) core protein (hbc) can be present in both nucleus and cytoplasm. the arginine-rich domain (ard) at the cytoplasmic tail of hbc contains both a nuclear localization signal (nls) and nuclear export signal (nes). we established a homokaryon assay to detect the dynamic trafficking of hbc between nucleus and cytoplasm in hepatocytes. using immunofluorescence assay (ifa) and peg-induced cell-cell fusion, we demonstrated that a chimeric reporter protein of sv40 large t antigen, ... | 2017 | 27975307 |
| intracytoplasmic transport of hepatitis b virus capsids. | the early steps of hbv entry remain largely unknown despite the recent discovery of an hbv-specific entry receptor. following entry hbv capsids have to be transported through the cytoplasm to the nuclear periphery, followed by nuclear entry. these steps have to take place in a coordinated manner to allow delivery of the genome into the nucleus. due to the viscosity of the cytoplasm, the intracytoplasmic translocation has to be active and directed.here, we describe protocols that can be applied t ... | 2017 | 27975306 |
| live cell imaging confocal microscopy analysis of hbv myr-pres1 peptide binding and uptake in ntcp-gfp expressing hepg2 cells. | to obtain basic knowledge about specific molecular mechanisms involved in the entry of pathogens into cells is the basis for establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread. lack of information about key cellular factors involved in the initial steps of hbv infection has hampered the characterization of hbv binding and entry for decades. however, recently, the liver-specific sodium-dependent taurocholate cotransporting polypeptide (ntc ... | 2017 | 27975305 |
| hepatitis b virus infection of heparg cells, heparg-hntcp cells, and primary human hepatocytes. | investigations of virus-host interactions rely on suitable in vitro cell culture systems that efficiently support virus infection. such systems should ideally provide conditions that resemble those of natural host cells, e.g., the cell-type specific signaling and metabolic pathways. for hbv infection, primary human hepatocytes (phhs) are the most faithful system fulfilling these requirements but access to these cells is limited. moreover, the reproducibility of experimental results depends on ma ... | 2017 | 27975304 |
| ntcp-reconstituted in vitro hbv infection system. | sodium taurocholate cotransporting polypeptide (ntcp) has been identified as a functional receptor for hepatitis b virus (hbv). expressing human ntcp in human hepatoma hepg2 cells (hepg2-ntcp) renders these cells susceptible for hbv infection. the hepg2-ntcp stably transfected cell line provides a much-needed and easily accessible platform for studying the virus. hepg2-ntcp cells could also be used to identify chemicals targeting key steps of the virus life cycle including hbv covalent closed ci ... | 2017 | 27975303 |
| new pharmacological strategies in rheumatic diseases. | targeting the pathogenic pathway of chronic inflammation represents an unmet challenge for controlling disease activity, preventing functional disability, and maintaining an adequate quality of life in patients with rheumatic diseases. abatacept, a novel molecule that inhibits co-stimulation signal, induces an inhibitory effect on the t-cells. this will further interfere with the activity of several cell lines, leading to the normalization of the immune response. in the latest years, abatacept h ... | 2017 | 27974925 |
| mean arterial pressure drop is an independent risk factor of death in patients with hbv-related cirrhosis ascites. | in patients with cirrhosis ascites, mean arterial pressure (map) is a credible sign of circulatory dysfunction. there are no studies on the relationship between map and long-term prognosis in hepatitis b virus (hbv)-related liver cirrhosis ascites. therefore, we assessed the association between map and prognosis in patients with liver cirrhosis ascites. | 2017 | 27991854 |
| existence of cancer stem cells in hepatocellular carcinoma: myth or reality? | the cancer stem cell (csc) hypothesis has been disproved in many cancers. cscs may exist in blood cancer, while many epithelial cancers may not have cscs but tumor-initiating cells (tics). several independent studies have provided strong evidence for existence of cscs in brain, skin, and colon cancers (mani et al. in cell 133:704-715, 2008, joseph et al. in cancer cell 13:129-140, 2008, reya et al. in nature 414:105-111, 2001), while the csc hypothesis remains controversial (magee et al. in canc ... | 2017 | 27990610 |
| optimization and synthesis of pyridazinone derivatives as novel inhibitors of hepatitis b virus by inducing genome-free capsid formation. | the capsid of hepatitis b virus (hbv) plays a vital role in virus dna replication. targeting nucleocapsid function has been demonstrated as an effective approach for anti-hbv drug development. a high-throughput screening and mechanism study revealed the hit compound 4a as an hbv assembly effector (aef), which could inhibit hbv replication by inducing the formation of hbv dna-free capsids. the subsequent sar study and drug-like optimization resulted in the discovery of the lead candidate 4r, with ... | 2017 | 27989113 |
| cervical human papillomavirus infection in the early postoperative period after liver transplantation: prevalence, risk factors, and concordance with anal infections. | solid organ transplant recipients are at increased risk of developing several human papillomavirus (hpv)-related malignancies, including cervical and anal cancers. the purpose of this prospective study was to assess the initial prevalence and risk factors for high-risk hpv (hr-hpv) cervical infections in liver transplant recipients, as well as their concordance with anal infections. a total of 50 female patients were enrolled in the department of general, transplant and liver surgery at the medi ... | 2017 | 27988990 |
| c-reactive protein-to-albumin ratio is a predictor of hepatitis b virus related decompensated cirrhosis: time-dependent receiver operating characteristics and decision curve analysis. | hepatitis b virus (hbv) infection remains a major health problem and hbv-related-decompensated cirrhosis (hbv-dc) usually leads to a poor prognosis. our aim was to determine the utility of inflammatory biomarkers in predicting mortality of hbv-dc. | 2017 | 27984322 |
| lhbs can elevate the expression of mdr1 through hif-1α in patients with chb infection: a comparative proteomic study. | hepatitis b virus (hbv) infection is a major risk factor for liver cirrhosis and hepatocellular carcinoma (hcc). to gain a better understanding of the pathogenesis of hbv infection, this study aimed to investigate the differentially expressed proteins (deps) in liver tissues from patients with chronic hepatitis b (chb) infection. | 2017 | 27999186 |
| metabolite identification of bentysrepinine (y101), a novel anti-hbv agent in rats using a five-step strategy based on a combined workflow with two different platforms of liquid chromatography-tandem mass spectrometry. | bentysrepinine (y101), a derivative of repensine (a compound isolated from dichondrarepens forst), is a novel phenyalanine dipeptide inhibiting dna-hbv and cccdna activities and is currently under development for the treatment of hepatitis b virus (hbv)-infected hepatitis. our previous study implied that there might be an existence of extensive metabolism of y101 in rats. therefore, it is necessary to perform metabolic profiling study to further evaluate its safety and drug-like properties. in t ... | 2017 | 27978466 |
| hepatitis b virus e antigen regulates monocyte function and promotes b lymphocyte activation. | hepatitis b virus (hbv) e (hbe) antigen is a nonstructural virus component with great immune regulation roles. it regulates adaptive immunity response and participates in persistent infection development. however, its roles on monocytes and b lymphocytes were rarely studied. herein, we studied hbe roles on u937 and hmy2.cir by creating hbe stably transfected cells using lentivirus. we detected the motility of hbe-u937 through transwell migration assay. cytokines that primarily produced by monocy ... | 2017 | 27976981 |
| long-term safety of anti-tnf agents on the liver of patients with spondyloarthritis and potential occult hepatitis b viral infection: an observational multicentre study. | the aim of the study was to retrospectively evaluate the long-term safety profile of anti-tumour necrosis factor (tnf)-α agents on the liver of patients with spondyloarthritis (spa) and a previously resolved hepatitis b virus (hbv) infection. | 2017 | 27974094 |
| synergistic interactions between hepatitis b virus rnase h antagonists and other inhibitors. | combination therapies are standard for management of human immunodeficiency virus (hiv) and hepatitis c virus (hcv) infections; however, no such therapies are established for human hepatitis b virus (hbv). recently, we identified several promising inhibitors of hbv rnase h (here simply rnase h) activity that have significant activity against viral replication in vitro here, we investigated the in vitro antiviral efficacy of combinations of two rnase h inhibitors with the current anti-hbv drug nu ... | 2017 | 27956427 |
| comparative characterization of hepatitis b virus surface antigen derived from different hepatitis b virus genotypes. | for human hepatitis b virus eight distinct and two candidate genotypes are described. these genotypes differ with respect to geographic distribution, molecular virology and virus-associated pathogenesis. comparative analysis of hbv genotypes revealed, with exception of hbv/g that shows impaired hbsag release, that no fundamental disparities between genotypes exist regarding glycosylation, subcellular distribution, release of hbsag and formation of subviral particles. however, there are distincti ... | 2017 | 27951436 |
| hbv/hcv co-infection is associated with a high level of hcv spontaneous clearance among drug users and blood donors in china. | understanding the biology of spontaneous clearance of hepatitis c virus (hcv) infection could lead to improved strategies to prevent the sequelae associated with chronic hcv infection. chronic infections with hepatitis virus are very common in china, but the factors associated with spontaneous clearance of hcv have not been adequately studied. we evaluated the spontaneous clearance of hcv among 1918 drug users and 1526 hcv-seropositive blood donors in guangzhou, china. among participants who wer ... | 2017 | 27943542 |
| involvement of viperin in prevention of intrauterine transmission of hepatitis b virus. | the aim of this study was to explore the role of viperin in the prevention of intrauterine infection of hepatitis b virus (hbv). placental samples were collected from seven hbv-positive pregnant women with their infants infected via intrauterine transmission (infected group), 30 hbv-positive women with non-infected infants (non-infected group), and 30 hbv-negative women (controls). the expression of viperin in placenta was analyzed with immunohistochemistry and western blotting. the expression o ... | 2017 | 27943419 |
| distinct hepatitis b virus integration patterns in hepatocellular carcinoma and adjacent normal liver tissue. | infection by the hepatitis b virus (hbv) is one of the main etiologies of hepatocellular carcinoma (hcc). during chronic infection, hbv dna can integrate into the human genome, and this has been postulated as a possible mechanism of hbv-induced hcc. in this study we used 2199 hbv integration sites from dr.vis v2.0 and mapped them to the human genome (hg19) to obtain viral integration sites (vis) related to protein-coding and non-protein-coding genes. in total, we found 1,377 and 767 vis within c ... | 2017 | 27943263 |
| risks and treatment strategies for de novo hepatitis b virus infection from anti-hbc-positive donors in pediatric living donor liver transplantation. | the aim of this study was to analyze the incidence and risk factors of de novo hbv infection in pediatric patients receiving living donor liver transplants (ldlt) from hbcab-positive donors, and to explore its treatment strategies. the data of 101 pediatric recipients receiving ldlt in tianjin first central hospital between september 2006 and december 2012 were retrospectively analyzed. the hbv markers were regularly tested before and after the surgery, including hbsab, hbsag, hbeag, hbeab, and ... | 2017 | 27933716 |
| advancing hepatitis b virus entry inhibitors. | 2017 | 27965159 | |
| stem cell-derived hepatocytes: a promising novel tool to study hepatitis b virus infection. | 2017 | 27965155 | |
| oxidative stress, a trigger of hepatitis c and b virus-induced liver carcinogenesis. | virally induced liver cancer usually evolves over long periods of time in the context of a strongly oxidative microenvironment, characterized by chronic liver inflammation and regeneration processes. they ultimately lead to oncogenic mutations in many cellular signaling cascades that drive cell growth and proliferation. oxidative stress, induced by hepatitis viruses, therefore is one of the factors that drives the neoplastic transformation process in the liver. this review summarizes current kno ... | 2017 | 27965466 |
| persistence of protective anti-hbs antibody levels and anamnestic response to hbv booster vaccination: a cross-sectional study among healthcare students 20 years following the universal immunization campaign in italy. | vaccination against hepatitis b virus (hbv) became mandatory in italy for all newborns and 12 years-old individuals in the 1991. the immunogenicity of hbv vaccine and the effectiveness of the universal immunization strategy have been widely demonstrated. however the need to assess the antibody concentrations above the well known serological correlate of protection for hbv infection (≥10 miu/ml), established in individuals immunized with a 3 doses vaccination course, is still recommended in subje ... | 2017 | 27925503 |
| hbx drives alpha fetoprotein expression to promote initiation of liver cancer stem cells through activating pi3k/akt signal pathway. | hepatitis b virus (hbv)-x protein (hbx) plays critical role in inducing the malignant transformation of liver cells. alpha fetoprotein (afp) expression is closely related to hepatocarcinogenesis. we report that oct4, klf4, sox2 and c-myc expression positively associated with afp(+)/hbv(+) hepatocellular carcinoma(hcc) tissues, and the expression of the stemness markers cd44, cd133 and epcam was significantly higher in afp(+)/hbv(+) hcc tissues compared to normal liver tissues or afp (-)/hbv(-) h ... | 2017 | 27925189 |
| susceptibility to vaccine-preventable diseases and vaccination adherence among healthcare workers in italy: a cross-sectional survey at a regional acute-care university hospital and a systematic review. | healthcare workers (hcws) have an increased risk both to acquire and to spread vaccine preventable diseases (vpds) both to their colleagues and, especially, to vulnerable patients. the prevention of occupational hazards among hcws is based on proper adoption of the standard and additional precautions, immunizations, and secondary preventive measures, such as post-exposure prophylaxis. moreover, hcws are often referred to as the most trusted source of vaccine-related information for their patient ... | 2017 | 27924688 |
| antiviral therapy inhibits viral reactivation and improves survival after repeat hepatectomy for hepatitis b virus-related recurrent hepatocellular carcinoma. | the aim of this study was to explore the impact of antiviral therapy (avt) on short- and long-term outcomes after rehepatectomy for patients with recurrent hepatitis b virus (hbv)-related hepatocellular carcinoma (hcc). | 2017 | 27923614 |
| the impact of emerging infectious diseases on chinese blood safety. | emerging infectious diseases (eids) have always been one of the major threats to public health. although the implementation of mandatory testing for 4 classical transfusion-transmitted infectious-human immunodeficiency virus, hepatitis b virus, hepatitis c virus, and syphilis-has reduced the transfusion risk of these pathogens, the potential threat of various eid agents and their constantly evolving variants to blood safety in china is not fully understood. this review presents 9 representative ... | 2017 | 27923518 |
| adherence to follow-up and treatment recommendations in greek and immigrant patients with chronic hepatitis b in greece. | immigrants have multiple barriers to access to health care systems. we evaluated the adherence to follow-up and treatment recommendations of chronic hepatitis b virus (hbv) greek and immigrant patients. | 2017 | 27922484 |
| severe de novo hepatitis b recovered from late-onset liver insufficiency with prolonged ascites and hypoalbuminemia due to hepatitis b virus genotype bj with precore mutation. | de novo hepatitis b is associated with a high risk of hepatic failure often resulting in fatal fulminant hepatitis even when nucleotide analogues are administered. a 77-year-old female developed de novo hepatitis b after r-chop (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisolone) treatment for diffuse large b-cell lymphoma. hepatitis b virus (hbv) isolated from the patient was of genotype bj, with a precore mutation (g1896a) exhibiting an extremely high viral load at the ons ... | 2017 | 27920641 |
| cpg methylation participates in regulation of hepatitis b virus gene expression in host sperm and sperm-derived embryos. | this study was undertaken to investigate relationship between hepatitis b virus (hbv) cpg methylation and hbv gene transcription in sperm and sperm-derived embryos. | 2017 | 27919171 |
| application of probabilistic modeling to quantify the reduction levels of hepatocellular carcinoma risk attributable to chronic aflatoxins exposure. | epidemiological studies show a definite connection between areas of high aflatoxin content and a high occurrence of human hepatocellular carcinoma (hcc). hepatitis b virus in individuals further increases the risk of hcc. the two risk factors are prevalent in rural kenya and continuously predispose the rural populations to hcc. a quantitative cancer risk assessment therefore quantified the levels at which potential pre- and postharvest interventions reduce the hcc risk attributable to consumptio ... | 2017 | 27918845 |
| hbsag may reappear following reactivation in individuals with spontaneous hbsag seroclearance 8 years previously. | hbsag reappearance may constitute not only a risk for liver disease but also an infectious source. we aimed to determine whether hbsag may reappear after spontaneous hbsag seroclearance. a cohort of 2999 hbsag-positive subjects aged 30-55 years was recruited in guangxi, china in 2004. hbsag was tested every 6 months from july 2004 to june 2007, then, one more time in december 2013. the results showed that spontaneous hbsag seroclearance occurred in 41 subjects in the first 3 years, giving a 0·54 ... | 2017 | 27917751 |
| usefulness of nucleic acid testing to reduce risk of hepatitis b virus transfusion-transmitted infection in argentina: high rate of recent infections. | results from 10-year experience using nucleic acid test (nat) screening in a blood bank of córdoba are presented, showing the first data on prevalence of recent hepatitis b virus (hbv) infections and occult hbv infections (obis) in argentina. | 2017 | 27917495 |
| hepatocellular carcinoma as an emerging morbidity in the thalassemia syndromes: a comprehensive review. | the incidence of hepatocellular carcinoma (hcc) in patients with thalassemia is on the rise. the 2 well recognized hcc risk factors in thalassemia are iron overload and chronic viral infection with hepatitis c. the carcinogenicity of iron is related to its induction of oxidative damage, which results in genotoxicity, and to immunologic dysregulation, which attenuates cancer immune surveillance. chronic hepatitis b and c infections lead to necroinflammation, which can prompt progression to hcc, b ... | 2017 | 27911488 |