Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| mitochondrial dna diversity of feral pigs from karukinka natural park, tierra del fuego island, chile. | control or eradication of exotic species is one of the greatest challenges facing biodiversity and ecosystem conservation. domestic pigs (sus scrofa domestica) were released and became feral in the southern region of chilean tierra del fuego island in the 1900s. currently, they inhabit part of karukinka natural park, an area of global conservation concern. to gain insight into the control of this invasive species, we analyzed genetic variation in the mitochondrial dna control region to determine ... | 2015 | 25966196 |
| evaluation of the distribution of paclitaxel by immunohistochemistry and nuclear magnetic resonance spectroscopy after the application of a drug-eluting balloon in the porcine ureter. | the urothelium represents "the tightest and most impermeable barrier in the body." we investigated the distribution of paclitaxel (ptx) in the ureteral wall after the inflation of a paclitaxel-eluting balloon (peb) in an attempt to elucidate the possibility of clinical application of pebs in the ureter. | 2015 | 25441059 |
| submucosal endoscopy as an aid to full-thickness resection: pilot study in the porcine stomach. | endoscopic full-thickness resection (eftr) is commonly performed with laparoscopic assistance. submucosal endoscopy with full-thickness resection (seftr) is a new technique that combines submucosal endoscopy with the mucosal safety valve flap method to enable eftr. | 2015 | 25440673 |
| adipose tissue remodeling in a novel domestic porcine model of diet-induced obesity. | to establish and characterize a novel domestic porcine model of obesity. | 2015 | 25627626 |
| ccaat-enhancer binding protein (c/ebp) β regulates insulin-like growth factor (igf) 1 expression in porcine liver during prenatal and postnatal development. | igf1 expression regulation attracts numerous interests because of its important role during mammalian growth and development. domestic pig can be used as a valuable animal model to investigate human development since they share the high similarity in general physiology and metabolism. in this study, we examined the expression pattern of igf1 and found it associated with liver c/ebp β expression pattern in porcine liver during embryonic and postnatal development. both igf1 and c/ebp β expression ... | 2015 | 25543525 |
| a tourniquet assisted cardiopulmonary resuscitation augments myocardial perfusion in a porcine model of cardiac arrest. | during cardiopulmonary resuscitation (cpr), myocardial blood flow generated by chest compression rarely exceeds 35% of its normal level. cardiac output generated by chest compression decreases gradually with the prolongation of cardiac arrest and resuscitation. early studies have demonstrated that myocardial blood flow during cpr is largely dependent on peripheral vascular resistance. in this study, we investigated the effects of chest compression in combination with physical control of peripher ... | 2015 | 25447436 |
| changes in lens stiffness due to capsular opacification in accommodative lens refilling. | accommodation may be restored to presbyopic lenses by refilling the lens capsular bag with a soft polymer. after this accommodative lens refilling prevention of capsular opacification is a requirement, since capsular opacification leads to a decreased clarity of the refilled lens. it has been hypothesized that capsular fibrosis causing the capsular opacification results in increased stiffness of the lens capsular bag, therewith contributing to a decrease in accommodative amplitude of the lens. h ... | 2015 | 25704214 |
| bipolar multi-electrode balloon catheter radiofrequency renal denervation with the vessix system: preclinical safety evaluation. | a bipolar multi-electrode 7 fr-compatible balloon-catheter radiofrequency (rf) renal denervation system (vessix™ renal denervation system; boston scientific, marlborough, ma, usa) was evaluated for safety in domestic swine. | 2015 | 25701511 |
| cardiac remodeling in a new pig model of chronic heart failure: assessment of left ventricular functional, metabolic, and structural changes using pet, ct, and echocardiography. | large animal models are needed to study disease mechanisms in heart failure (hf). in the present study we characterized the functional, metabolic, and structural changes of myocardium in a novel pig model of chronic myocardial infarction (mi) by using multimodality imaging and histology. | 2015 | 25698475 |
| randomized trial on the physiologic impact of notes. | our previous work revealed significantly less acidosis in swine undergoing natural orifice translumenal endoscopic surgery (notes) using endoscopic air insufflation than swine undergoing standard laparoscopy. we wanted to evaluate the differential effects of co2 versus intra-abdominal pressure as source for this finding. in addition, we investigated the endocrine stress response between swine undergoing notes peritoneoscopy with co2 insufflation and animals undergoing standard diagnostic laparos ... | 2015 | 25698397 |
| a taphonomic study exploring the differences in decomposition rate and manner between frozen and never frozen domestic pigs (sus scrofa). | this research examined differences in decomposition rate and manner of domestic pig subjects (sus scrofa) in never frozen (control) and previously frozen (experimental) research conditions. eight control and experimental subjects were placed in an identical outdoor research environment. daily quantitative and qualitative measurements were collected: abdominal circumference, total body score (tbs), temperature, photographs, descriptive decomposition stages, and visual observations. field necropsi ... | 2015 | 25693630 |
| modeling cardiac arrest and resuscitation in the domestic pig. | cardiac arrest remains a leading cause of death and permanent disability worldwide. although many victims are initially resuscitated, they often succumb to the extensive ischemia-reperfusion injury inflicted on the internal organs, especially the brain. cardiac arrest initiates a complex cellular injury cascade encompassing reactive oxygen and nitrogen species, ca(2+) overload, atp depletion, pro- and anti-apoptotic proteins, mitochondrial dysfunction, and neuronal glutamate excitotoxity, which ... | 2015 | 25685718 |
| two-in-one aortic valve sizing and valvuloplasty conductance balloon catheter. | inaccurate aortic valve sizing and selection is linked to paravalvular leakage in transcatheter aortic valve replacement (tavr). here, a novel sizing valvuloplasty conductance balloon (svcb) catheter is shown to be accurate, reproducible, unbiased, and provides real-time tool for aortic valve sizing that fits within the standard valvuloplasty procedure. | 2015 | 25510238 |
| comparison of osteoconductive properties of three different β-tricalcium phosphate graft materials: a pilot histomorphometric study in a pig model. | the aim of this study was to compare the de novo bone formation ability and osteoconductive effects of three different β-tricalcium phosphate (β-tcp) graft materials. the micro-architectural parameters of the newly formed bone tissues were also compared among the different graft materials. | 2015 | 25491275 |
| inhibition of nitric oxide synthases, but not inducible nitric oxide synthase, selectively worsens left ventricular function after successful resuscitation from cardiac arrest in swine. | nitric oxide (no) is a critical regulator of vascular tone and signal transduction in the cardiovascular system. no is synthesized by three unique enzymes (nitric oxide synthases [nos]): endothelial and neuronal nos, both constitutively expressed, and inducible nos (inos), which is induced by proinflammatory stimuli and subsequently produces a burst of no. no has been implicated as both an injurious and a beneficial mediator after cardiac arrest and resuscitation. a previous study in swine found ... | 2015 | 25639298 |
| pathological and molecular diagnosis of the 2013 african swine fever outbreak in lusaka, zambia. | african swine fever (asf) is a highly contagious and fatal hemorrhagic viral disease of domestic pigs. the disease is widespread in sub-saharan africa and has repeatedly been introduced into other continents. the current study describes the diagnostic investigations of a hemorrhagic disease that was reported in pigs in lusaka (october 2013), zambia. necropsy, histopathology, and molecular diagnosis using polymerase chain reaction and sequence analysis confirmed the disease to be asf. the sequenc ... | 2015 | 25535151 |
| safety and biocompatibility of a novel self-expanding nitinol carotid stent with hybrid cell design in a porcine model of neointimal hyperplasia. | stent design may influence the outcomes, suggesting that adverse event rates vary according to free cell area and cell design. open cell design technology of self-expandable stents, dedicated for carotid revascularisation has better deliverability, although closed cell technology is expected to cause fewer thromboembolic events. | 2015 | 25371312 |
| advances in research in animal models of burn-related hypertrophic scarring. | skin burn injuries affect approximately 500,000 people per year in france. after deep burns, functional sequelae associated with hypertrophic and retractile scars are an important public health problem. to understand the pathophysiology of sequelae and evaluate new therapeutic approaches, the use of animal models that should be standard tools is necessary. some pre-clinical models of hypertrophic scars after burns have been described, but the choice of the appropriate and relevant experimental m ... | 2015 | 25356852 |
| preliminary observations of arthropods associated with buried carrion on oahu. | several studies in hawaii have focused on arthropod succession and decomposition patterns of surface remains, but the current research presents the first study to focus on shallow burials in this context. three domestic pig carcasses (sus scrofa l.) were buried at the depths of 20-40 cm in silty clay loam soil on an exposed ridge on the leeward side of the volcanically formed koolau mountain range. one carcass was exhumed after 3 weeks, another after 6 weeks, and the last carcass was exhumed aft ... | 2015 | 25413711 |
| haemodynamic differences between pancuronium and vecuronium in an experimental pig model. | to compare baseline cardiovascular function in anesthetised pigs using either pancuronium or vecuronium as a neuromuscular blocker. | 2015 | 24985148 |
| efficacy and safety of novel epicardial circumferential left atrial ablation with pulmonary vein isolation in sustained atrial fibrillation. | the aim of this study was to examine the efficacy and safety of this novel epicardial circumferential left atrial ablation (claa) with pulmonary vein isolation (pvi) in sustained atrial fibrillation (af). thirty domestic pigs were divided equally into 3 groups: af without ablation (af group), af with pvi (pvi group), and af with claa and pvi (claa + pvi group). af was induced by rapid atrial pacing. after af was induced, claa and pvi were performed for pigs in claa + pvi group, and pvi was perfo ... | 2015 | 25323730 |
| immunohistochemical evidence of leptin and its receptor in the carpal glands of domestic pigs and wild boar. | in recent years, the protein hormone leptin has been the subject of numerous studies designed to clarify and interpret its functional significance; it has been speculated that this goes well beyond the control of appetite and energy metabolism. in particular, the presence of leptin and its receptor has been observed in various glands anatomically and functionally related to the reproductive apparatus. this has led to the hypothesis that leptin may act directly in the functional control of these ... | 2015 | 25292333 |
| generation of crispr/cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. | the domestic pig has been widely used as an important large animal model. precise and efficient genetic modification in pig provides a great promise in biomedical research. recently, clustered regularly interspaced short palindromic repeat (crispr)/crispr-associated (cas) system has been successfully used to produce many gene-targeted animals. however, these animals have been generated by co-injection of cas9 mrna and single-guide rna (sgrna) into one-cell stage embryos, which mostly resulted in ... | 2015 | 25274063 |
| qtl for seed protein and amino acids in the benning × danbaekkong soybean population. | we identified qtl associated with protein and amino acids in a soybean mapping population that was grown in five environments. these qtl could be used in mas to improve these traits. soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. as a sole dietary source of protein, soybean is deficient in the amino acids lysine (lys), threonine (thr), methionine (met), and cysteine (cys). increasi ... | 2015 | 25673144 |
| genetic variation of the east balkan swine (sus scrofa) in bulgaria, revealed by mitochondrial dna and y chromosomal dna. | east balkan swine (ebs) sus scrofa is the only aboriginal domesticated pig breed in bulgaria and is distributed on the western coast of the black sea in bulgaria. to reveal the breed's genetic characteristics, we analysed mitochondrial dna (mtdna) and y chromosomal dna sequences of ebs in bulgaria. nucleotide diversity (πn ) of the mtdna control region, including two newly found haplotypes, in 54 ebs was higher (0.014 ± 0.007) compared with that of european (0.005 ± 0.003) and asian (0.006 ± 0.0 ... | 2015 | 25662601 |
| light microscopy and scanning electron microscopy study on microstructure of gallbladder mucosa in pig. | the present light microscopy (lm) and scanning electron microscopy (sem) studies on porcine gallbladder mucosa provide a description of the microstructures of great functional importance such as mucosal folds, the epithelium, glands, and lymphatic nodules. the results showed the regional structural differences of the porcine gallbladder wall. depending on the part of the gallbladder, three types of mucosal structures were described: simple and branched folds and mucosal crypts. an important stru ... | 2015 | 25604381 |
| profiles of reproductive hormone in the microminipig during the normal estrous cycle. | we investigated changes in the peripheral plasma concentrations of ovarian steroids and gonadotropins throughout the estrous cycle of the microminipig (mmpig), a novel experimental animal model. the mean (± standard error of the mean (sem)) duration of estrous and the estrous cycle in seven animals was 66.0 ± 5.2 h and 20.6 ± 0.3 days, respectively. a luteinizing hormone (lh) surge was observed 16.5 ± 6.5 h after the onset of estrous in six out of the seven animals. the lh peak and the duration ... | 2015 | 25600524 |
| the effects of hemorrhagic shock secondary to hepatectomy in a swine model. | ischemia-reperfusion injury caused by severe hemorrhagic shock and subsequent resuscitation leads to deterioration of hepatic homeostasis and possibly to liver failure. the present study focuses on determining whether there is a different biological response to hemorrhagic shock by different sources of hemorrhage, hepatic hemorrhage (hh) versus peripheral hemorrhage. | 2015 | 25659614 |
| mechanism by which shock wave lithotripsy can promote formation of human calcium phosphate stones. | human stone calcium phosphate (cap) content correlates with higher urine cap supersaturation (ss) and urine ph as well as with the number of shock wave lithotripsy (swl) treatments. swl does damage medullary collecting ducts and vasa recta, sites for urine ph regulation. we tested the hypothesis that swl raises urine ph and therefore cap ss, resulting in cap nucleation and tubular plugging. the left kidney (t) of nine farm pigs was treated with swl, and metabolic studies were performed using bil ... | 2015 | 25656372 |
| domestic pigs' (sus scrofa domestica) use of direct and indirect visual and auditory cues in an object choice task. | recently, foraging strategies have been linked to the ability to use indirect visual information. more selective feeders should express a higher aversion against losses compared to non-selective feeders and should therefore be more prone to avoid empty food locations. to extend these findings, in this study, we present a series of studies investigating the use of direct and indirect visual and auditory information by an omnivorous but selective feeder-the domestic pig. subjects had to choose bet ... | 2015 | 25650328 |
| quantitative characterization of endothelial cell morphologies depending on shear stress in different blood vessels of domestic pigs using a focused ion beam and high resolution scanning electron microscopy (fib-sem). | microstructured surfaces mimicking the endothelial cell (ec) morphology is a new approach to improve the blood compatibility of synthetic vascular grafts. the ecs are capable of changing their shapes depending on different shear conditions. however, the quantitative correlation between ec morphology and shear stress has not yet been investigated statistically. the aim of this study was to quantitatively investigate the morphology of ecs in dependence on the shear stress. blood flow rates in diff ... | 2015 | 25622890 |
| adaptation and possible ancient interspecies introgression in pigs identified by whole-genome sequencing. | domestic pigs have evolved genetic adaptations to their local environmental conditions, such as cold and hot climates. we sequenced the genomes of 69 pigs from 15 geographically divergent locations in china and detected 41 million variants, of which 21 million were absent from the dbsnp database. in a genome-wide scan, we identified a set of loci that likely have a role in regional adaptations to high- and low-latitude environments within china. intriguingly, we found an exceptionally large (14- ... | 2015 | 25621459 |
| liposarcoma in animals: literature review and case report in a domestic pig (sus scrofa). | liposarcomas are malignant tumors of adipocytes. the current report describes a liposarcoma in a 2.5-year-old, mixed-breed commercial sow that was detected during meat inspection. on gross examination, a firm, whitish, multinodular, 20 cm ×10 cm mass was observed in the perirenal area along with smaller nodules multifocally scattered within the renal parenchyma. histological examination revealed an anaplastic sarcoma with clear intracytoplasmic lipidic vacuoles that were positive for sudan black ... | 2015 | 25613042 |
| sodium nitroprusside-enhanced cardiopulmonary resuscitation facilitates intra-arrest therapeutic hypothermia in a porcine model of prolonged ventricular fibrillation. | the aim of this study was to assess the effect of sodium nitroprusside-enhanced cardiopulmonary resuscitation on heat exchange during surface cooling. we hypothesized that sodium nitroprusside-enhanced cardiopulmonary resuscitation would decrease the time required to reach brain temperature less than 35°c compared to active compression-decompression plus impedance threshold device cardiopulmonary resuscitation alone, in the setting of intra-cardiopulmonary resuscitation cooling. we further hypot ... | 2015 | 25525755 |
| allogenic ipsc-derived rpe cell transplants induce immune response in pigs: a pilot study. | stem cell strategies focused on replacement of rpe cells for the treatment of geographic atrophy are under intense investigation. although the eye has long been considered immune privileged, there is limited information about the immune response to transplanted cells in the subretinal space of large animals. the purpose of this study was to evaluate the survival of allogenic induced pluripotent stem cell-derived rpe cells (ipsc-rpe) delivered to the subretinal space of the pig as well as determi ... | 2015 | 26138532 |
| [18f]fdg accumulation in early coronary atherosclerotic lesions in pigs. | inflammation is an important contributor to atherosclerosis progression. a glucose analogue 18f-fluorodeoxyglucose ([18f]fdg) has been used to detect atherosclerotic inflammation. however, it is not known to what extent [18f]fdg is taken up in different stages of atherosclerosis. we aimed to study the uptake of [18f]fdg to various stages of coronary plaques in a pig model. | 2015 | 26120829 |
| flexible growing rods: a pilot study to determine if polymer rod constructs may provide stability to skeletally immature spines. | surgical treatments for early onset scoliosis (eos), including growing rod constructs, involve many complications. some are due to biomechanical factors. a construct that is more flexible than current instrumentation systems may reduce complications. the purpose of this preliminary study was to determine spine range of motion (rom) after implantation of simulated growing rod constructs with a range of clinically relevant structural properties. the hypothesis was that rom of spines instrumented w ... | 2015 | 25810752 |
| wild boars from sweden, austria, the czech republic and japan possess intact mannose-binding lectin 2 (mbl2) genes. | the two-nucleotide deletion recently detected in the mannose-binding lectin 2 gene in purebred and crossbred domestic pigs was not found among 68 wild boars representing 4 populations from europe and asia. this suggests that the deletion is a result of breeding and/or genetic drift/bottle necks. | 2015 | 25809846 |
| atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. | atrial fibrillation (af) and heart failure (hf) are two of the most common cardiovascular diseases. they often coexist and account for significant morbidity and mortality. alterations in cellular ca2+ homeostasis play a critical role in af initiation and maintenance. this study was designed to specifically elucidate af-associated remodeling of atrial ca2+ cycling in the presence of mild hf. af was induced in domestic pigs by atrial burst pacing. the animals underwent electrophysiologic and echoc ... | 2015 | 25775120 |
| establishing a reproducible hypertrophic scar following thermal injury: a porcine model. | our complete understanding of hypertrophic scarring is still deficient, as portrayed by the poor clinical outcomes when treating them. to address the need for alternative treatment strategies, we assess the swine animal burn model as an initial approach for immature scar evaluation and therapeutic application. | 2015 | 25750848 |
| crimped braided sleeves for soft, actuating arm in robotic abdominal surgery. | this paper investigates different types of crimped, braided sleeve used for a soft arm for robotic abdominal surgery, with the sleeve required to contain balloon expansion in the pneumatically actuating arm while it follows the required bending, elongation and diameter reduction of the arm. | 2015 | 25744663 |
| tissue concentrations of sulfamethazine and tetracycline hydrochloride of swine (sus scrofa domestica) as it relates to withdrawal methods for international export. | the use of water medications is a common practice in the us swine industry to treat and prevent infections in swine herds with minimal labor and without risk of needle breakage. there are concerns that fda-approved withdrawal times (wdt) may be inadequate for several water medications when exporting pork products to countries where mrls (maximum residue limits) are lower than us tolerance levels. in this study, withdrawal intervals (wdi) were estimated for pigs when dosed with tetracycline and s ... | 2015 | 25707857 |
| evaluation of the plasmatic and parenchymal elution kinetics of two different irinotecan-loaded drug-eluting embolics in a pig model. | to evaluate and compare irinotecan elution kinetics of two drug-eluting embolic agents in a porcine model. | 2015 | 25704223 |
| introducer development for coronary sinus access from parasternal mediastinotomy. | right parasternal mediastinotomy with right atriotomy has been used clinically for pacemaker insertion. a similar approach might facilitate access to the coronary sinus for biventricular pacing and other manipulations when more conventional approaches are not feasible. the primary barrier to this is lack of appropriate introducers and techniques. | 2015 | 26181586 |
| experimental study of temperature-sensitive chitosan/β-glycerophosphate embolic material in embolizing the basicranial rete mirabile in swines. | the aim of the present study was to evaluate the feasibility of the non-adhesive temperature-sensitive liquid embolic material, chitosan/β-glycerophosphate (c/gp), in embolizing the basicranial rete mirabile (rem) in a swine model of cerebral arteriovenous malformation (cavm). a total of 24 domestic swines were used as the experimental animals, among which 12 pigs underwent direct embolization of one side of the rem, while the other 12 pigs underwent embolization of the bilateral rem following a ... | 2015 | 26170955 |
| vasopressin infusion with small-volume fluid resuscitation during hemorrhagic shock promotes hemodynamic stability and survival in swine. | current management of hemorrhagic shock (hs) in the battlefield and civilian settings favors small-volume fluid resuscitation before controlling the source of bleeding. we investigated in a swine model of hs the effects of vasopressin infusion along with small-volume fluid resuscitation; with erythropoietin (epo) and hs severity as additional factors. | 2015 | 26107942 |
| the interactive effect of the degradation of cotton clothing and decomposition fluid production associated with decaying remains. | textiles are a commonly encountered source of evidence in forensic cases. in the past, most research has been focused on how textiles affect the decomposition process while little attention has been paid to how the decomposition products interact with the textiles. while some studies have shown that the presence of remains will have an effect on the degradation of clothing associated with a decaying body, very little work has been carried out on the specific mechanisms that prevent or delay text ... | 2015 | 26074165 |
| expression of vpac1 receptor at the level of mrna and protein in the porcine female reproductive system. | the presence and distribution of vasoactive intestinal polypeptide (vip) receptor vpac1 was studied in the ovary, oviduct and uterus (uterine horn and cervix) of the domestic pig using methods of molecular biology (rt-pcr and immunoblot) and immunohistochemistry. the expression of vpac1 receptor at mrna level was confirmed with rt-pcr in all the studied parts of the porcine female reproductive system by the presence of 525 bp pcr product and at the level of proteins by the detection of 46 kda pr ... | 2015 | 25928928 |
| fluid dynamics simulation of right ventricular outflow tract oversizing. | repair of the right ventricular outflow tract (rvot) in paediatric cardiac surgery remains challenging due to the high reoperation rate. intimal hyperplasia and consequent arteriosclerosis is one of the most important limitation factors for graft durability. since local shear stress and pressure are predictive elements for intimal hyperplasia and wall degeneration, we sought to determine in an oversized 12-mm rvot model, with computed fluid dynamics simulation, the local haemodynamical factors t ... | 2015 | 25912476 |
| real-time in-vivo imaging of pulmonary capillary perfusion using probe-based confocal laser scanning endomicroscopy in pigs: an interventional laboratory study. | little is known about real-time in-vivo microscopy of pulmonary capillary perfusion because current microscopy requires direct access to lung tissue with surgical intervention such as the thoracic-window technique and open-lung model. | 2015 | 25906262 |
| tvp1022: a novel cardioprotective drug attenuates left ventricular remodeling after ischemia/reperfusion in pigs. | the current cornerstone treatment of myocardial infarction (mi) is restoration of coronary blood flow by means of thrombolytic therapy or primary percutaneous coronary intervention. however, reperfusion of ischemic myocardium can actually provoke tissue damage, defined as "ischemia-reperfusion (i/r) injury." tvp1022 [the s-isomer of rasagiline (azilect), fda-approved anti-parkinson's drug] was found to exert cardioprotective activities against various cardiac insults, such as chronic heart failu ... | 2015 | 25900266 |
| pluripotent and metabolic features of two types of porcine ipscs derived from defined mouse and human es cell culture conditions. | the domestic pig is an excellent animal model for stem cell research and clinical medicine. there is still no suitable culture condition to generate authentic porcine embryonic stem cells (pescs) and high quality porcine induced pluripotent stem cells (pipscs). in this study, we found that culture conditions affected pluripotent and metabolic features of pipscs. using defined human embryonic stem cell (hesc) and mouse esc (mesc) culture conditions, we generated two types of pipscs, one of which ... | 2015 | 25893435 |
| development of a new lamp assay for the detection of csfv strains from cuba: a proof-of-concept study. | classical swine fever (csf) is a devastating animal disease of great economic impact worldwide. in many countries, csf has been endemic for decades, and vaccination of domestic pigs is one of the measures to control the disease. consequently, differentiating infected from vaccinated animals by antibody elisa screening is not applicable. in some countries, such as cuba, lack of molecular techniques for sensitive, rapid and reliable detection of virus genomes is a critical point. to overcome this ... | 2015 | 25877822 |
| analysis of carcass and meat quality traits and nutritional values of hybrid wild boars under different crossing systems. | the aim of this study was to analyze the results of two crossing systems between wild boars and different domesticated pig breeds. hybrid wild boars were produced by crossing captured wild boars with meishan pigs and ly sows according to the traditional production system. the resultant commercial hybrids were black and white in coat color, respectively. significant differences were found in the carcass and meat quality traits and nutritional values between these two hybrid wild boars. compared w ... | 2015 | 25867408 |
| the effects of altered maxillary growth on patterns of mandibular rotation in a pig model. | a thorough understanding of influence of maxillary growth on patterns of mandibular rotation during development is important with regard to the treatment of skeletal discrepancies. in the present study, we examined whether experimentally altered maxillary position has a significant influence on patterns of mandibular rotation in a pig model. | 2015 | 25841069 |
| the chimerical genome of isla del coco feral pigs (costa rica), an isolated population since 1793 but with remarkable levels of diversity. | the history of domestic species and of their wild ancestors is not a simple one, and feral processes can clarify key aspects of this history, including the adaptive processes triggered by new environments. here, we provide a comprehensive genomic study of isla del coco (costa rica) feral pigs, a unique population that was allegedly founded by two individuals and has remained isolated since 1793. using snp arrays and genome sequencing, we show that cocos pigs are hybrids between asian and europea ... | 2015 | 25827466 |
| effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in european domestic pigs. | parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (snps) make them another important marker for pedigree tracing. here, we compared the effectiveness of microsatellites and ... | 2015 | 25730075 |
| evidence for a link between tail biting and central monoamine metabolism in pigs (sus scrofa domestica). | tail biting in pigs is a major welfare problem within the swine industry. even though there is plenty of information on housing and management-related risk factors, the biological bases of this behavioral problem are poorly understood. the aim of this study was to investigate a possible link between tail biting, based on behavioral recordings of pigs during an ongoing outbreak, and certain neurotransmitters in different brain regions of these pigs. we used a total of 33 pigs at a farm with a lon ... | 2015 | 25728243 |
| reply to 'on genetic differentiation between domestic pigs and tibetan wild boars'. | 2015 | 25711862 | |
| on genetic differentiation between domestic pigs and tibetan wild boars. | 2015 | 25711861 | |
| mitochondrial dna sequence and phylogenetic evaluation of geographically disparate sus scrofa breeds. | next generation sequencing of mitochondrial dna (mtdna) facilitates studies into the metabolic characteristics of production animals and their relation to production traits. sequence analysis of mtdna from pure-bred swine with highly disparate production characteristics (mangalica blonde, mangalica swallow-bellied, meishan, turopolje, and yorkshire) was initiated to evaluate the influence of mtdna polymorphisms on mitochondrial function. herein, we report the complete mtdna sequences of five sus ... | 2015 | 25153451 |
| voluderm microneedle technology for skin treatments-in vivo histological evidence. | the growing demand for skin rejuvenation procedures with minimal down time and low risk has led to the development of fractional radiofrequency (rf) systems. the new voluderm™ rf microneedle technology creates minute columns of tissue thermal microablation. treatment triggers natural fractional healing, resulting in dermal volumizing and skin renewal. this preclinical research assessed the safety and efficacy of the voluderm through histological evaluation of morphological changes in the target ... | 2015 | 25151912 |
| active dynamic infrared thermal imaging in burn depth evaluation. | the aim of this study was to find the relationship between active dynamic thermography (adt) with cold excitation and burn depth. this new modality of evaluation of burns seems to be an attractive proposal for quantitative classification, allowing proper choice of burn wound treatment: conservative or surgical, especially compared with static thermography. the work was an in vivo experiment on domestic pigs, and a small number of patients were also diagnosed as part of the study. statistical ana ... | 2015 | 25144810 |
| preclinical randomised safety, efficacy and physiologic study of the silicon dioxide inert-coated axetis and bare metal stent: short-, mid- and long-term outcome. | to evaluate the short-, mid- and long-term safety, efficacy and vascular physiology of axetis silicon dioxide (sio2, abrading the micropores) inert-coated stent implantation in a randomised preclinical setting. | 2015 | 24769439 |
| histopathological comparative analysis of peri-implant soft tissue response after dental implant placement with flap and flapless surgical technique. experimental study in pigs. | the aim of this study was comparing the effect of flapless vs. flap technique of implant placement on inflammation degree of peri-implant soft tissue, through histopathological analysis. | 2015 | 25039366 |
| genome-wide analysis reveals artificial selection on coat colour and reproductive traits in chinese domestic pigs. | pigs from asia and europe were independently domesticated from c. 9000 years ago. during this period, strong artificial selection has led to dramatic phenotypic changes in domestic pigs. however, the genetic basis underlying these morphological and behavioural adaptations is relatively unknown, particularly for indigenous chinese pigs. here, we performed a genome-wide analysis to screen 196 regions with selective sweep signals in tongcheng pigs, which are a typical indigenous chinese breed. gene ... | 2015 | 25132237 |
| computed tomography for planning and postoperative imaging of transvenous mitral annuloplasty: first experience in an animal model. | to investigate the use of computed tomography (ct) to measure the mitral valve annulus size before implantation of a percutaneous mitral valve annuloplasty device in an animal trial. seven domestic pigs underwent ct before and after implantation of a cardioband™ (a percutaneously implantable mitral valve annuloplasty device) with a second-generation 128-section dual-source ct machine. implantation of the cardioband™ was performed in a standard fashion according to a protocol. animals were sacrif ... | 2015 | 25119889 |
| effects of amlodipine and perindoprilate on the structure and function of mitochondria in ventricular cardiomyocytes during ischemia-reperfusion in the pig. | the aim of this study was to determine whether amlodipine and/or perindoprilate injected intravenously (iv) prior to ischemia exerted protective effects on mitochondria structural and functional alterations induced by ischemia and aggravated by reperfusion. heart rate, the duration of monophasic action potentials (dmap), peak of the time derivative of left ventricular pressure (lv dp/dt max), mitochondria structural and functional parameters in the left ventricle ischemic area were measured afte ... | 2015 | 24588464 |
| qualitative tissue differentiation by analysing the intensity ratios of atomic emission lines using laser induced breakdown spectroscopy (libs): prospects for a feedback mechanism for surgical laser systems. | the research work presented in this paper focuses on qualitative tissue differentiation by monitoring the intensity ratios of atomic emissions using 'laser induced breakdown spectroscopy' (libs) on the plasma plume created during laser tissue ablation. the background of this study is to establish a real time feedback control mechanism for clinical laser surgery systems during the laser ablation process. ex-vivo domestic pig tissue samples (muscle, fat, nerve and skin) were used in this experimen ... | 2015 | 24376030 |
| immunohistochemical analysis of blood vessels in peri-implant mucosa: a comparison between mini-incision flapless and flap surgeries in domestic pigs. | the aim of this experimental study is to compare the effect of mini-incision flapless versus flap technique of implant placement on the amount of vascular structures and blood vessel elements in peri-implant soft tissue, using immunohistochemical analysis. | 2015 | 24438481 |
| dynamic changes in nuclear import of a nuclear localisation signal-bearing substrate in 8-cell stage porcine embryos. | coordinated intracellular trafficking is critically important for proper timing of major cellular events during embryogenesis. nuclear import mediated by the karyopherin α/β (importin α/β) heterodimer is perhaps the best characterised nuclear trafficking system in eukaryotic cells. seven karyopherin α subtypes have been identified in the domestic pig, and although each karyopherin α subtype transports proteins bearing classical nuclear localisation signals (nlss), individual karyopherin α subtyp ... | 2015 | 24342392 |
| dental implant macro-design features can impact the dynamics of osseointegration. | the purpose of this study was to compare the clinical performance of two dental implant types possessing a different macro-design in the in vivo pig model. | 2015 | 24238310 |
| assessing the risk of african swine fever introduction into the european union by wild boar. | the presence of african swine fever (asf) in the caucasus region and russian federation has increased concerns that wild boars may introduce the asf virus into the european union (eu). this study describes a semi-quantitative approach for evaluating the risk of asf introduction into the eu by wild boar movements based on the following risk estimators: the susceptible population of (1) wild boars and (2) domestic pigs in the country of origin; the outbreak density in (3) wild boars and (4) domest ... | 2015 | 23926953 |
| estimating the basic reproductive number (r0) for african swine fever virus (asfv) transmission between pig herds in uganda. | african swine fever (asf) is a highly contagious, lethal and economically devastating haemorrhagic disease of domestic pigs. insights into the dynamics and scale of virus transmission can be obtained from estimates of the basic reproduction number (r0). we estimate r0 for asf virus in small holder, free-range pig production system in gulu, uganda. the estimation was based on data collected from outbreaks that affected 43 villages (out of the 289 villages with an overall pig population of 26,570) ... | 2015 | 25938429 |
| a lentiviral vector expressing japanese encephalitis virus-like particles elicits broad neutralizing antibody response in pigs. | japanese encephalitis virus (jev) is the major cause of viral encephalitis in southeast asia. vaccination of domestic pigs has been suggested as a "one health" strategy to reduce viral disease transmission to humans. the efficiency of two lentiviral trip/jev vectors expressing the jev envelope prm and e glycoproteins at eliciting protective humoral response was assessed in a mouse model and piglets. | 2015 | 26437302 |
| pbmc transcriptomic responses to primary and secondary vaccination differ due to divergent lean growth and antibody titers in a pig model. | the genetic relationship between immune responsiveness and performance is not well understood, but a major topic of the evolution of resource allocation and of relevance in human medicine and livestock breeding, for instance. this study aims to show differences of transcript abundance changes during the time intervals before and after two tetanus toxoid (tt) vaccinations in domestic pigs differing in lean growth (lg) and anti-tt-antibody titers (ab) parameters of performance and immunocompetence ... | 2015 | 26175500 |
| standardization of the experimental autoimmune myasthenia gravis (eamg) model by immunization of rats with torpedo californica acetylcholine receptors--recommendations for methods and experimental designs. | myasthenia gravis (mg) with antibodies against the acetylcholine receptor (achr) is characterized by a chronic, fatigable weakness of voluntary muscles. the production of autoantibodies involves the dysregulation of t cells which provide the environment for the development of autoreactive b cells. the symptoms are caused by destruction of the postsynaptic membrane and degradation of the achr by igg autoantibodies, predominantly of the g1 and g3 subclasses. active immunization of animals with ach ... | 2015 | 25796590 |
| combined inhibition of complement and cd14 improved outcome in porcine polymicrobial sepsis. | sepsis is an exaggerated and dysfunctional immune response to infection. activation of innate immunity recognition systems including complement and the toll-like receptor family initiate this disproportionate inflammatory response. the aim of this study was to explore the effect of combined inhibition of the complement component c5 and the toll-like receptor co-factor cd14 on survival, hemodynamic parameters and systemic inflammation including complement activation in a clinically relevant porci ... | 2015 | 26612199 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| redox state of flavin adenine dinucleotide drives substrate binding and product release in escherichia coli succinate dehydrogenase. | the complex ii family of enzymes, comprising respiratory succinate dehydrogenases and fumarate reductases, catalyzes reversible interconversion of succinate and fumarate. in contrast to the covalent flavin adenine dinucleotide (fad) cofactor assembled in these enzymes, soluble fumarate reductases (e.g., those from shewanella frigidimarina) that assemble a noncovalent fad cannot catalyze succinate oxidation but retain the ability to reduce fumarate. in this study, an sdha-h45a variant that elimin ... | 2015 | 25569225 |
| bat and pig ifn-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses. | ifn-induced transmembrane protein 3 (ifitm3) is a restriction factor that blocks cytosolic entry of numerous viruses that utilize acidic endosomal entry pathways. in humans and mice, ifitm3 limits influenza-induced morbidity and mortality. although many ifitm3-sensitive viruses are zoonotic, whether ifitms function as antiviral restriction factors in mammalian species other than humans and mice is unknown. here, ifitm3 orthologues in the microbat (myotis myotis) and pig (sus scrofa domesticus) w ... | 2015 | 25614588 |
| epidemiology of leptospirosis in africa: a systematic review of a neglected zoonosis and a paradigm for 'one health' in africa. | leptospirosis is an important but neglected bacterial zoonosis that has been largely overlooked in africa. in this systematic review, we aimed to summarise and compare current knowledge of: (1) the geographic distribution, prevalence, incidence and diversity of acute human leptospirosis in africa; and (2) the geographic distribution, host range, prevalence and diversity of leptospira spp. infection in animal hosts in africa. | 2015 | 26368568 |
| serosurvey of smooth brucella, leptospira spp. and toxoplasma gondii in free-ranging jaguars (panthera onca) and domestic animals from brazil. | this study investigated the exposure of jaguar populations and domestic animals to smooth brucella, leptospira spp. and toxoplasma gondii in the cerrado, pantanal and amazon biomes of brazil. between february 2000 and january 2010, serum samples from 31 jaguars (panthera onca), 1,245 cattle (bos taurus), 168 domestic dogs (canis lupus familiaris) and 29 domestic cats (felis catus) were collected and analysed by rose bengal test for smooth brucella, microscopic agglutination test for leptospira s ... | 2015 | 26605787 |
| experimental infection of ornithodoros erraticus sensu stricto with two portuguese african swine fever virus strains. study of factors involved in the dynamics of infection in ticks. | african swine fever (asf) is a frequently devastating hemorrhagic disease of domestic pigs and wild boar and ornithodoros erraticus sensu stricto argasid ticks are the only biological vectors of african swine fever virus (asfv) known to occur in europe. recently this disease emerged in eastern europe and russian federation, showing a huge potential for a rapid spread between countries. there is some risk of re-emergence of asf in the countries where these ticks exist, that can contribute for the ... | 2015 | 26366570 |
| anaplasma sp. and hemoplasma infection in leopard cats (prionailurus bengalensis euptilurus) from korea. | this study examined the occurrence of anaplasma spp. and hemoplasma infection in leopard cats, prionailurus bengalensis euptilurus, in korea. twenty-nine biological samples were tested by molecular analysis. two (6.9%) and eight (27.6%) tested specimens were positive for anaplasma bovis and hemoplasma infection, respectively. based on our results, anaplasma/ehrlichia spp. and hemoplasma are regularly infecting leopard cat populations of korea. considering their endangered status, regular monitor ... | 2015 | 26040618 |
| serological prevalence of viral agents that induce reproductive failure in south korean wild boar. | viral agents associated with reproductive failure such as aujeszky's disease virus (adv), encephalomyocarditis virus (emcv), and porcine parvovirus (ppv) have also been identified in european wild boar. to screen for the presence of antibodies against adv, emcv, and ppv from wild boar (sus scrofa) in south korea, 481 serum samples were collected from wild boar hunted between december 2010 and may 2011. | 2015 | 25888836 |
| single nucleotide markers of d-loop for identification of indian wild pig (sus scrofa cristatus). | partial fragment of d-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (snp) markers in the region. the paper also reappraises snp markers from two fragments of cytochrome b gene and a fragment 12s rrna gene distinguishing the indian wild pig from other pig species of the world. | 2015 | 27047129 |
| genome-wide association analyses reveal significant loci and strong candidate genes for growth and fatness traits in two pig populations. | recently, genome-wide association studies (gwas) have been reported on various pig traits. we performed a gwas to analyze 22 traits related to growth and fatness on two pig populations: a white duroc × erhualian f2 intercross population and a chinese sutai half-sib population. | 2015 | 25885760 |
| investigating the role of free-ranging wild boar (sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: a pathological, prevalence and risk-factor study. | enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae has a significant economic impact on domestic pig production. a control program carried out from 1999 to 2003 successfully reduced disease occurrence in domestic pigs in switzerland, but recurrent outbreaks suggested a potential role of free-ranging wild boar (sus scrofa) as a source of re-infection. since little is known on the epidemiology of ep in wild boar populations, our aims were: (1) to estimate the prevalence of m. hyopneumoniae ... | 2015 | 25747151 |
| exploring the genetic signature of body size in yucatan miniature pig. | since being domesticated about 10,000-12,000 years ago, domestic pigs (sus scrofa domesticus) have been selected for traits of economic importance, in particular large body size. however, yucatan miniature pigs have been selected for small body size to withstand high temperature environment and for laboratory use. this renders the yucatan miniature pig a valuable model for understanding the evolution of body size. we investigate the genetic signature for selection of body size in the yucatan min ... | 2015 | 25885114 |
| staphylococcus aureus induces hypoxia and cellular damage in porcine dermal explants. | we developed a porcine dermal explant model to determine the extent to which staphylococcus aureus biofilm communities deplete oxygen, change ph, and produce damage in underlying tissue. microelectrode measurements demonstrated that dissolved oxygen (do) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while do levels for biofilm-infected tissue declined sharply from the surface, with no measurable oxygen detectable in the underlying dermal tissue. magnetic resonance imaging demonstrated ... | 2015 | 25847960 |
| the relation between catheter occlusion and backflow during intraparenchymal cerebral infusions. | background/aims: the distribution of infusate into the brain by convection-enhanced delivery can be affected by backflow along the catheter shaft. this work assesses the following: (1) whether tissue coring and occlusion of the catheter lumen occurs when an open end-port catheter is inserted, (2) whether there is a relationship between intracatheter pressure and backflow, and (3) whether catheter occlusion increases backflow. methods: freshly excised monkey brains were used to assess tissue cori ... | 2015 | 25721097 |
| when pigs fly, ucp1 makes heat. | brown and beige adipose tissue may represent important therapeutic targets for the treatment of diabetes and obesity as these organs dissipate nutrient energy as heat through the thermogenic uncoupling protein 1 (ucp1). while mice are commonly used to mimic the potential effects of brown/beige adipose tissue that may act in human metabolism, new animal models are edging into the market for translational medicine. pigs reflect human metabolism better than mice in multiple parameters such as obesi ... | 2015 | 25973382 |
| corrigendum: cellular scaling rules for the brain of artiodactyla include a highly folded cortex with few neurons. | [this corrects the article on p. 128 in vol. 8, pmid: 25429261.]. | 2015 | 25859187 |
| phenotype and animal domestication: a study of dental variation between domestic, wild, captive, hybrid and insular sus scrofa. | identifying the phenotypic responses to domestication remains a long-standing and important question for researchers studying its early history. the great diversity in domestic animals and plants that exists today bears testament to the profound changes that domestication has induced in their ancestral wild forms over the last millennia. domestication is a complex evolutionary process in which wild organisms are moved to new anthropogenic environments. although modern genetics are significantly ... | 2015 | 25648385 |
| snp marker discovery in koala tlr genes. | toll-like receptors (tlrs) play a crucial role in the early defence against invading pathogens, yet our understanding of tlrs in marsupial immunity is limited. here, we describe the characterisation of nine tlrs from a koala immune tissue transcriptome and one tlr from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes. we surveyed genetic diversity in 20 koalas from new south wales, australia and showed that one gene, tlr10 ... | 2015 | 25799012 |
| a large animal model of spinal muscular atrophy and correction of phenotype. | spinal muscular atrophy (sma) is caused by reduced levels of survival motor neuron (smn) protein, which results in motoneuron loss. therapeutic strategies to increase smn levels including drug compounds, antisense oligonucleotides, and scaav9 gene therapy have proved effective in mice. we wished to determine whether reduction of smn in postnatal motoneurons resulted in sma in a large animal model, whether sma could be corrected after development of muscle weakness, and the response of clinically ... | 2015 | 25516063 |
| gene coexpression networks reveal key drivers of phenotypic divergence in porcine muscle. | domestication of the wild pig has led to obese and lean phenotype breeds, and evolutionary genome research has sought to identify the regulatory mechanisms underlying this phenotypic diversity. however, revealing the molecular mechanisms underlying muscle phenotype variation based on differentially expressed genes has proved to be difficult. to characterize the mechanisms regulating muscle phenotype variation under artificial selection, we aimed to provide an integrated view of genome organizati ... | 2015 | 25651817 |
| experimental infection with the toxoplasma gondii me-49 strain in the brazilian br-1 mini pig is a suitable animal model for human toxoplasmosis. | toxoplasma gondii causes toxoplasmosis, a worldwide disease. experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. br-1 mini pigs were intramuscularly infected with t. gondii with tachyzoites (rh strain) or orally infected with cysts (me-49 strain). haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. no alterations were observed in erythrocyte and platelet values; ... | 2015 | 25742268 |
| effect of body mass and clothing on carrion entomofauna. | carcass mass largely affects pattern and rate of carrion decomposition. supposedly, it is similarly important for carrion entomofauna; however, most of its likely effects have not been tested experimentally. here, simultaneous effects of carcass mass and clothing are analyzed. a factorial block experiment with four levels of carcass mass (small carcasses 5-15 kg, medium carcasses 15.1-30 kg, medium/large carcasses 35-50 kg, large carcasses 55-70 kg) and two levels of carcass clothing (clothed an ... | 2015 | 25874664 |