Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| molecular cloning, tissue distribution, and ontogeny of mrna expression of growth hormone in orange-spotted grouper (epinephelus coioides). | a full-length cdna encoding for growth hormone (gh) was cloned from orange-spotted grouper (epinephelus coioides) pituitary using reverse transcription and rapid amplification of cdna ends (race). the gh precursor cdna consists of 956 bp in size with a 85 bp 5'-untranslated region and 259 bp 3'-untranslated region. the 612 bp open reading frame encodes a 204 amino acid (aa) protein, which represents the precursor of grouper gh composed of a 17 aa signal peptide followed by a 187 aa mature gh pol ... | 2005 | 16055124 |
| virulence factors and pathogenicity of hafnia alvei for gilthead seabream, sparus aurata l. | virulence factors (eae gene, haemolytic capacity, fimbriae, resistance to the bactericidal effect of serum, siderophore production) and pathogenicity for gilthead seabream, sparus aurata l., were analysed for 23 hafnia alvei strains. none of the strains used in ld50 studies were lethal for seabream at doses as high as >10(8) cfu ml(-1). in chronic challenge studies differences in severity of the inflammatory response were observed between strains. on the basis of correlation of the inflammatory ... | 2005 | 16083446 |
| production and characterisation of gilthead sea bream (sparus auratus) recombinant parathyroid hormone related protein. | the production and purification of gilthead sea bream recombinant parathyroid hormone related protein [sbpthrp(1-125)] using an escherichia coli system and one step purification process with continuous elution gel electrophoresis is reported. the cdna encoding sbpthrp(1-125) was cloned into a prokaryotic expression vector pet-11a. the recombinant plasmid was used to transfect e. coli bl21(de3) plyss and sbpthrp(1-125) synthesis was induced by addition of 1mm isopropyl-beta-d-thiogalactopyranosid ... | 2005 | 15993105 |
| evaluating the effects of protection on fish predators and sea urchins in shallow artificial rocky habitats: a case study in the northern adriatic sea. | man-made defence structures (e.g., breakwaters, jetties) are becoming common features of marine coastal landscapes all around the world. the ecology of assemblages of species associated with such artificial structures is, however, poorly known. in this study, we evaluated the density and size of fish predators of echinoids (i.e., diplodus sargus, diplodus vulgaris, sparus aurata), and the density of sea urchins (i.e., paracentrotus lividus) at defence structures (i.e., breakwaters) inside and ou ... | 2005 | 15589985 |
| epidemiology of cryptosporidium molnari in spanish gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) cultures: from hatchery to market size. | a long-term epidemiological study of cryptosporidium molnari in aquacultured european sea bass (esb) and gilthead sea bream (gsb) was performed in different types of facilities on the atlantic, cantabric, and mediterranean coasts. four types of studies were carried out. in study a, fish raised from juveniles to marketable size (ongrowing stage) were periodically sampled in three different types of cultures. studies b and c focused on hatchery and nursery facilities. in study d, occasional sampli ... | 2005 | 15640180 |
| early innate immune response and redistribution of inflammatory cells in the bony fish gilthead seabream experimentally infected with vibrio anguillarum. | an obvious difference between the immune system of fish and mammals is that fish lack both bone marrow and lymph nodes; in their place, the head-kidney acts as a major haematopoietic and lymphoid organ in adult fish, whereas the thymus, spleen and mucosa-associated lymphoid tissues are common to both fish and mammals. this suggests that differences exist in antigen presentation and naive lymphocyte stimulation, a prerequisite for the initiation of adaptive immune responses. intraperitoneal injec ... | 2005 | 15714279 |
| evaluation of candidate probiotic strains for gilthead sea bream larvae (sparus aurata) using an in vivo approach. | the aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (sparus aurata). | 2005 | 15752217 |
| isolation of lymphocystis disease virus from sole, solea senegalensis kaup, and blackspot sea bream, pagellus bogaraveo (brunnich). | two viruses were isolated from cultured sole, solea senegalensis, and wild blackspot sea bream, pagellus bogaraveo, and preliminarily characterized as lymphocystis disease viruses (lcdvs). viral isolates were characterized by morphological, biochemical and biophysical properties. in addition, the susceptibility of four fish cell lines was also tested. lcdv isolates developed cytopathic effects on the saf-1 cell line at 5 and 6 days post-infection and reached titres of 10(6) tcid50 ml(-1). the an ... | 2005 | 15813864 |
| adhesion to sole, solea senegalensis kaup, mucus of microorganisms isolated from farmed fish, and their interaction with photobacterium damselae subsp. piscicida. | abstract most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. however, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. nineteen isolates obtained from farmed senegalese sole, solea senegalensis kaup, and gilthead sea bream, sparus aurata l., have been evaluated for their cap ... | 2005 | 15813865 |
| ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, sparus aurata. | the aim of the study was to elucidate the pattern of changes in the whole body concentrations of thyroid hormones (th) and cortisol in eggs, pre-larvae, and larvae in the gilthead sea bream, sparus aurata. the ontogeny of these hormones was related to specific morphological characteristics that characterize early development. th and cortisol were detected in substantial amounts in the eggs of sea bream. t3 concentration in eggs was found to be higher than that of t4 (t3, 4.02+/-0.38 ng g-1; t4, ... | 2005 | 15862562 |
| the effect of sea bream (sparus aurata) broodstock and larval vaccination on the susceptibility by photobacterium damsela subsp. piscicida and on the humoral immune parameters. | sea bream broodstock were immunised 1 or 2 months before spawning with a novel photobacteriosis vaccine. sixty-seven-day-old larvae (mean weight 22.3 mg) originating from immunised and non-immunised parents were experimentally infected with the photobacterium damsela subsp. piscicida (phdp). larvae from immunised fish showed delayed onset and lower mortality (66.67%) compared with larvae from control fish (80%). eighty-nine-day-old larvae (mean weight 162.2 mg) from both groups were bath vaccina ... | 2005 | 15863015 |
| measurement of pthrp, pthr1, and casr expression levels in tissues of sea bream (sparus aurata) using quantitative pcr. | a quantitative pcr (q-pcr) method has been established to measure the mrna expression levels of parathyroid hormone-related protein (pthrp), parathyroid hormone receptor type 1 (pthr1), and calcium-sensing receptor (casr) in sea bream (sparus aurata), using the housekeeping gene, beta-actin, as endogenous control. taqman primers and probes were designed using the primer express program, according to the published/unpublished sequences of the three target genes and beta-actin of sea bream. differ ... | 2005 | 15891056 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| vaccination strategies to prevent emerging diseases for spanish aquaculture. | in recent years, three serious diseases have emerged in spanish aquaculture. these are lactococcosis caused by lactococcus garvieae, which is of economical importance in rainbow trout (oncorhynchus mykiss); pseudomonadiasis caused by pseudomonas anguilliseptica which affects gilthead seabream (sparus aurata) and turbot (scophthalmus maximus); and flexibacteriosis caused by tenacibaculum maritimum which became a devastating problem in the emerging culture of sole (solea spp). to obtain useful inf ... | 2005 | 15962472 |
| effects of lactoferrin on non-specific immune responses of gilthead seabream (sparus auratus l.). | the main innate cellular immune responses of gilthead seabream (sparus auratus l.) leucocytes were evaluated after in vitro incubation with human lactoferrin (lf). isolated head-kidney leucocytes were incubated with 0 (control) to 1 mg ml(-1) lf-supplemented culture medium for 30, 120, 240 or 360 min and assayed for viability, peroxidase content, and respiratory burst, phagocytic and cytotoxic activities. only respiratory burst activity was found to increase when using the highest lf concentrati ... | 2005 | 15475308 |
| interactions of microorganisms isolated from gilthead sea bream, sparus aurata l., on vibrio harveyi, a pathogen of farmed senegalese sole, solea senegalensis (kaup). | four bacterial isolates from farmed gilthead sea bream, sparus aurata, included in a previous study as members of the vibrionaceae and pseudomonodaceae and the genus micrococcus, have been evaluated for their adhesive ability to skin and intestinal mucus of farmed senegalese sole, solea senegalensis, and their antagonistic effect on vibrio harveyi, a pathogen of sole. these isolates showed higher adhesion to sole mucus than the pathogenic strains of v. harveyi assayed. only two of the isolates s ... | 2005 | 16266326 |
| kudoa iwatai (myxosporea: multivalvulida) in wild and cultured fish in the red sea: redescription and molecular phylogeny. | gilt-head sea bream, sparus aurata l., the mediterranean's most important mariculture species, has been cultured for the last 30 yr in eilat (israeli red sea). kudoa sp. was the first myxosporean parasite reported from this species. in recent years, an increase in prevalence in both land-based and sea-cage facilities in eilat has been observed. infections with the same kudoa species appeared in cultured european sea bass dicentrarchus labrax (l.) and grey mullet mugil cephalus in the same farms, ... | 2005 | 16419765 |
| in vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses. | the potential effect of the intraperitoneal or dietary administration of propolis, a honeybee product, on gilthead seabream (sparus aurata l.) innate immune responses was evaluated. fish were intraperitoneally injected with 5mg of water (wep), ethanol (eep) or both (wep + eep) extracts of propolis and sampled after 1, 3, 5 and 10 days. when administered in the diet, propolis was dissolved in ethanol and added to a pellet diet at a concentration of 0, 0.1 or 10 g kg(-1) diet, the fish being sampl ... | 2005 | 15450970 |
| alpha2-macroglobulin in the marine fish sparus aurata. | the alpha2-macroglobulin proteinase inhibitors (alpha2ms) are a family of plasma proteins with the unique ability to inhibit a broad spectrum of proteinases, but are also known as binding proteins for many growth factors and cytokines, including growth hormone and members of the transforming growth factor-beta superfamily. a partial cdna (475 amino acids) encoding the c-terminus of alpha2m was cloned from the liver of the marine teleostean fish sparus aurata. the deduced amino acid sequence of t ... | 2005 | 16054852 |
| calcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptor. | the scales of bony fish represent a significant reservoir of calcium but little is known about their contribution, as well as of bone, to calcium balance and how calcium deposition and mobilization are regulated in calcified tissues. in the present study we report the action of parathyroid hormone-related protein (pthrp) on calcium mobilization from sea bream (sparus auratus) scales in an in vitro bioassay. ligand binding studies of piscine 125i-(1-35(tyr))pthrp to the membrane fraction of isola ... | 2005 | 16181689 |
| identification and analysis of teleost slow muscle troponin t (stnt) and intronless tnt genes. | in the present study cdna clones representing two slow skeletal muscle troponin t genes (stnt1sb and stnt2sb) in the sea bream (sparus auratus), an important aquaculture species, were isolated and characterised. a third, intronless, tnt gene (itntsb), which is an apparent orthologue of a previously described zebrafish tnt, was also isolated. in adult sea bream stnt expression was restricted to red muscle and, using northern blotting, a single low abundance transcript was identified for stnt1sb ( ... | 2005 | 16168583 |
| interactive effects of high stocking density and food deprivation on carbohydrate metabolism in several tissues of gilthead sea bream sparus auratus. | the influence of high stocking density (hsd) and food deprivation was assessed on carbohydrate metabolism of several tissues of gilthead sea bream sparus auratus for 14 days. fish were randomly assigned to one of four treatments: (1) fed fish under normal stocking density (nsd) (4 kg m(-3)); (2) fed fish under hsd (70 kg m(-3)); (3) food-deprived fish under nsd; and (4) food-deprived fish under hsd. after 14 days, samples were taken from the plasma, liver, gills, kidney and brain for the assessm ... | 2005 | 16106404 |
| gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cdna microarray technology. | large-scale gene expression studies were performed for one of the main european aquaculture species, the gilthead sea bream sparus auratus l. for this purpose, a cdna microarray containing 10,176 clones from a cdna library of mixed embryonic and larval stages was constructed. in addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. however, so far, no lar ... | 2005 | 16046618 |
| branchial osmoregulatory response to salinity in the gilthead sea bream, sparus auratus. | the branchial osmoregulatory response of gilthead sea bream (sparus auratus l.) to short-term (2-192 hr) and long-term (2 weeks) exposure to different environmental salinities (5 per thousand, 15 per thousand, 25 per thousand, 38 per thousand and 60 per thousand) was investigated. a "u-shaped" relationship was observed between environmental salinity and gill na+,k+ -atpase activity in both long- and short-term exposure to altered salinity, with the increase in activity occurring between 24 and 9 ... | 2005 | 15945079 |
| evaluation of gilthead sea bream, sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes. | cryopreservation produces several types of damage in spermatozoa, leading to fertility impairment. the reduction arises both from a lower viability post-thaw and from sublethal dysfunctions in some of the surviving cells. in the present study, we have analysed the effect of cryopreservation in 5 ml macrotubes on the quality of post-thawed gilthead sea bream sperm. several standard sperm quality parameters were determined: ph and osmolarity of seminal plasma, sperm concentration, and motility. an ... | 2005 | 15925579 |
| parasite communities of adriatic cage-reared fish. | from june 2001 to march 2002, 7 semi-offshore facilities in the adriatic sea rearing sea bass dicentrarchus labrax, sea bream sparus aurata, sharpsnout bream diplodus puntazzo and red sea bream pagellus bogaraveo aged 1+, were monitored for the presence of protozoan and metazoan parasites. obtained data sets of abundance and prevalence were used to evaluate the structure and dynamic of infra- and component parasite communities. in all hosts, except the red sea bream, dominant parasites were mono ... | 2005 | 15900691 |
| sea bream (sparus auratus) estrogen receptors: phylogeny and tissue distribution. | most estrogen actions are mediated by two estrogen receptor subtypes (eralpha and erbeta). while a single erbeta appears to be present in higher vertebrates, two forms of erbeta encoded by different genes have recently been isolated in some teleost fish species. we investigated whether this also applies to the hermaphrodite sparid sea bream (sparus auratus) and cloned a second erbeta (sberbeta2) in this species. we have also compared the tissue distribution of the three receptors as yet identifi ... | 2005 | 15891082 |
| the involvement of thyroid hormone metabolism in gilthead sea bream (sparus auratus) osmoregulation. | we have investigated the effect of adaptation to low salinity water on the thyroid status of the euryhaline teleost, sparus auratus. we show that, following low salinity adaptation, the plasma t(4) concentration increases and branchial deiodination activities of t(4), t(3), and rt(3) decrease. moreover, branchial and hepatic enzyme activities that are putatively involved in thyroid hormone metabolism respond differentially in low salinity conditions. our results indicate the involvement of thyro ... | 2005 | 15891062 |
| duplication of growth hormone receptor (ghr) in fish genome: gene organization and transcriptional regulation of ghr type i and ii in gilthead sea bream (sparus aurata). | nucleotide sequences encoding for functional growth hormone receptors (ghr) are now available in salmonids (coho and masu salmon) and other fish orders. several authors have hypothesized a divergent evolution of salmonid ghrs, but the recent finding that a trout cdna is related to non-salmonid (ghr type i) rather than to salmonid ghrs (ghr type ii) points out a possible duplication of actively transcribed ghr genes. to address this issue, we search by rt-pcr for ghr type ii in trout, gilthead se ... | 2005 | 15862563 |
| ontogeny of osteonectin expression in embryos and larvae of sea bream (sparus auratus). | osteonectin (osn) is a glycoprotein which is implicated in development, bone formation and mineralisation, tumorigenesis, angiogenesis, and wound healing. regulation of its expression by hormones may be one of the mechanisms by which the endocrine system affects bone metabolism. as a first step to understanding osn function in fish, the gene expression of the recently cloned cdna for sea bream, sparus auratus, osteonectin (sbosn) was characterised during embryonic and larval development. sbosn m ... | 2005 | 15862559 |
| evaluation of dna damage in rainbow trout (oncorhynchus mykiss) and gilthead sea bream (sparus aurata) cryopreserved sperm. | cryopreservation causes several types of damage to spermatozoa, such as loss of plasma membrane integrity and functionality, loss of motility, and atp content, resulting in decrease of fertility rates. this spermatozoal damage has been widely investigated for several marine and freshwater fish species. however, not much attention has been paid to the nuclear dna. the objective of this study was to determine the degree to which cryopreservation induces spermatozoal dna damage in two commercially ... | 2005 | 15843004 |
| comparison of volatiles of cultured and wild sea bream (sparus aurata) during storage in ice by dynamic headspace analysis/gas chromatography-mass spectrometry. | cultured and wild sea bream were compared for differences in their volatile components over a 23 day storage period in ice. a total of 60 compounds in cultured and 78 compounds in wild sea bream were tentatively identified (in addition to this, there were 23 unknowns in cultured and 29 unknowns in wild sea bream volatiles). these included aldehydes, ketones, alcohols, aromatics, terpenes, furans, sulfur-containing compounds, an acid, and miscellaneous compounds. although selection of best fish i ... | 2005 | 15796602 |
| three peroxisome proliferator-activated receptor isotypes from each of two species of marine fish. | the cloning and characterization of cdnas and genes encoding three peroxisome proliferator-activated receptor (ppar) isotypes from two species of marine fish, the plaice (pleuronectes platessa) and the gilthead sea bream (sparus aurata), are reported for the first time. although differences in the genomic organization of the fish ppar genes compared with their mammalian counterparts are evident, sequence alignments and phylogenetic comparisons show the fish genes to be homologs of mammalian ppar ... | 2005 | 15790725 |
| isolation and characterization of piscine osteonectin and downregulation of its expression by pth-related protein. | the skeleton is the main source of osteonectin mrna in adults of the seawater teleost sea bream sparus auratus. it is expressed by cells forming the basement membrane of calcifying tissue indicating that, as in mammals, it may play a role in osteoblast differentiation. pthrp induced downregulation of osteonectin mrna in vitro in scales, a mineralizing tissue with bone-like metabolism. this indicates a means to redirect calcium to activities such as vitellogenesis when this ion is in high demand. | 2005 | 15765188 |
| hormonal regulation of hepatic igf-i and igf-ii gene expression in the marine teleost sparus aurata. | the present work aimed to determine whether gnrh potentiates the effect of growth hormone (gh) on insulin-like growth factors (igf-i and igf-ii) hepatic gene expression in sparus aurata liver. since several hepatic genes were shown to underlie direct regulation via the hepatic estrogen receptor, another aim was to extend our understanding of direct estrogen effects on liver igfs gene expression. pre-reproductive sea bream females were treated with gh, gnrh, estradiol-17beta, gh plus gnrh, and es ... | 2005 | 15736123 |
| time course of osmoregulatory and metabolic changes during osmotic acclimation in sparus auratus. | changes in different osmoregulatory and metabolic parameters over time were assessed in gills, kidney, liver and brain of gilthead sea bream sparus auratus transferred either from seawater (sw, 38 p.p.t.) to hypersaline water (hsw, 55 p.p.t.) or from sw to low salinity water (lsw, 6 p.p.t.) for 14 days. changes displayed by osmoregulatory parameters revealed two stages during hyperosmotic and hypo-osmotic acclimation: (i) an adaptive period during the first days of acclimation (1-3 days), with i ... | 2005 | 16272252 |
| food-deprivation induces hsp70 and hsp90 protein expression in larval gilthead sea bream and rainbow trout. | heat shock proteins (hsps) expression is commonly used as indicators of cellular stress in animals. however, very little is known about either the expression patterns of hsps or their role in the stress-tolerance phenomenon in early life stages of fish. to this end, we examined the impact of food-deprivation (12 h), reduced oxygen levels (3.5 mg/l for 1 h) and heat shock (hs: +5 degrees c for 1 h) on hsp70 and hsp90 protein expression in early life stages of the gilthead sea bream (sparus aurata ... | 2005 | 16257553 |
| stimulation of cortisol release by the n terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro. | the mode of action of pthrp in the regulation of sea bream (sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system. piscine (1-34)pthrp (10(-6)-10(-11) m) stimulated cortisol production in a dose-dependent manner. the ed50 of (1-34)pthrp was 2.8 times higher than that of (1-39)acth, and maximum increase in cortisol production in response to 10(-8) m of (1-34)pthrp was approximately 7-fold lower than for 10(-8) m of (1-39)acth. in contrast to (1-34) ... | 2005 | 15459121 |
| osmoregulatory action of 17beta-estradiol in the gilthead sea bream sparus auratus. | the osmoregulatory action of 17beta-estradiol (e2) was examined in the euryhaline teleost sparus auratas. in a first set of experiments, fish were injected once with vegetable oil containing e2 (1, 2 and 5 microg/g body weight), transferred 12h after injection from sea water (sw, 38 ppt salinity) to hypersaline water (hsw, 55 ppt) or to brackish water (bw, 5 ppt salinity) and sampled 12h later (i.e. 24 h post-injection). in a second experiment, fish were injected intraperitoneally with coconut o ... | 2004 | 15449347 |
| nutritional assessment of somatolactin function in gilthead sea bream (sparus aurata): concurrent changes in somatotropic axis and pancreatic hormones. | the role of somatolactin (sl) in the regulation of energy homeostasis in gilthead sea bream (sparus aurata) has been analysed. first, a down-regulation of plasma sl levels in response to gross shifts in dietary amino acid profile and the graded replacement of fish meal by plant protein sources (50%, 75% and 100%) has been observed. thus, the impaired growth performance with changes in dietary amino acid profile and dietary protein source was accompanied by a decrease in plasma sl levels, which a ... | 2004 | 15369843 |
| histological alterations in the liver of sea bream, sparus aurata l., caused by short- or long-term feeding with vegetable oils. recovery of normal morphology after feeding fish oil as the sole lipid source. | this study evaluated the effects of fish oil (fo) replacement by vegetable oils [soybean oil (so), rapeseed oil (ro), linseed oil (lo)] and subsequent feeding with fo on the liver morphology of sea bream. a short-term trial (3 months) and long-term trial (6 months) were carried out feeding sea bream with the following experimental diets: fo100%; so60% + fo40%; ro60% +fo40%; lo60% + fo40%; so + ro +lo60% + fo40%. finally, all groups from the long-term trial were fed with fo100% for 95 days (washo ... | 2004 | 15357712 |
| adaptation of lipid metabolism, tissue composition and flesh quality in gilthead sea bream (sparus aurata) to the replacement of dietary fish oil by linseed and soyabean oils. | linseed (lo) and soyabean (so) oils were evaluated as fish-oil (fo) substitutes in the diets of marketable-sized gilthead sea bream (sparus aurata). practical diets were designed factorially with the lipid added as follows (%): fo 100, lo 60+fo 40, lo 80+fo 20, so 60+fo 40, so 80+fo 20. the effects of experimental diets on growth, fatty acids patterns in liver and muscle, flesh quality variables and activities of selected enzymes involved in lipid synthesis and catabolism were determined at the ... | 2004 | 15230986 |
| molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from gilthead sea bream (sparus aurata): response of its mrna levels and glucokinase expression to refeeding and diet composition. | to examine the relationship between structure and function of glucose-6-phosphatase (g6pase) in fish, we undertook molecular cloning and modulation of g6pase expression by starvation and refeeding on diets with different nutrient composition in the liver of the carnivorous fish, sparus aurata. a cdna encoding the full-length g6pase catalytic subunit from the liver of s. aurata was isolated. this cdna encodes a 350-amino acid protein, with low homology to the mammalian g6pase, although it contain ... | 2004 | 15193269 |
| the n-terminal sequence directs import of mitochondrial alanine aminotransferase into mitochondria. | herein, we report cloning and subcellular localization of two alanine aminotransferase (alt) isozymes, calt and malt, from liver of gilthead sea bream (sparus aurata). cho cells transfected with constructs expressing calt or malt as c- or n-terminal fusion with the enhanced green fluorescent protein (egfp) showed that calt is cytosolic, whereas malt localized to mitochondria. fusion of egfp to malt n-terminus or removal of amino acids 1-83 of malt avoided import into mitochondria, supporting evi ... | 2004 | 15147904 |
| glomerulonephritis and immunosuppression associated with dietary essential fatty acid deficiency in gilthead sea bream, sparus aurata l., juveniles. | an experiment was conducted to determine the effect of an essential fatty acid (efa) deficient diet on growth, immune status and renal morphology of juvenile gilthead sea bream, using two diets: a control diet containing 2% (dw) of n-3 high unsaturated fatty acid (n-3 hufa) and a diet formulated to be deficient in efa for this species and containing 0.5% dw of n-3 hufa (diet nfa). after 9 weeks of feeding the efa-deficient diet fish showed a reduction in growth compared with the control group (1 ... | 2004 | 15139908 |
| isolation of a novel aquaglyceroporin from a marine teleost (sparus auratus): function and tissue distribution. | the aquaporins (formerly called the major intrinsic protein family) are transmembrane channel proteins. the family includes the chip group, which are functionally characterised as water channels and the glp group, which are specialised for glycerol transport. the present study reports the identification and characterisation of a novel glp family member in a teleost fish, the sea bream sparus auratus. a sea bream aquaporin (sbaqp) cdna of 1047 bp and encoding a protein of 298 amino acids was isol ... | 2004 | 14978062 |
| development of two bone-derived cell lines from the marine teleost sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix gla protein and osteocalcin. | a growing interest in the understanding of the ontogeny and mineralization of fish skeleton has emerged from the recent implementation of fish as a vertebrate model, particularly for skeletal development. whereas several in vivo studies dealing with the regulation of bone formation in fish have been published, in vitro studies have been hampered because of a complete lack of fish-bone-derived cell systems. we describe here the development and the characterization of two new cell lines, designate ... | 2004 | 14762709 |
| cloning and analysis of expression of a gilthead sea bream (sparus aurata) mx cdna. | in the current work, we have cloned and sequenced the full cdna for a mx protein in the gilthead sea bream (sparus aurata) by race pcr. the mx cdna of 2182 bp contained an open reading frame of 1857 bp that codes for a protein of 618 aa. within the coding sequence, characteristic features of mx proteins were found, such as a tripartite guanosine-5'-triphosphate (gtp)-binding motif (gxxxsgks/t, dxxg and t/nkxd), the signature of the dynamin family, lprg(s/k)givtr, and a sequence that codes for a ... | 2004 | 14675830 |
| hormonal regulation of vasa-like messenger rna expression in the ovary of the marine teleost sparus aurata. | the vasa gene is an important maternal regulator of primordial germ cell (pgc) development in both vertebrate and invertebrate models. it is also expressed in the mature gonads, but its role in these tissues is still unclear. in oviparous species, oogenesis is a complex process under hormonal control: estrogens, gonadotropins, and other hormones operate at different stages of oogenesis, regulating meiosis, vitellogenesis, follicle maturation, and egg release. the aim of this work is the determin ... | 2004 | 14613903 |
| na-dependent d-glucose transport by intestinal brush border membrane vesicles from gilthead sea bream (sparus aurata). | brush border membrane vesicles (bbmv) enriched in sucrase, maltase and alkaline phosphatase, and impoverished in na(+)-k(+)-atpase, were isolated from proximal and distal intestine of the gilthead sea bream (sparus aurata) by a mgcl(2) precipitation method. vesicles were suitable for the study of the characteristics of d-glucose apical transport. only one d-glucose carrier was found in vesicles from each intestinal segment. in both cases, the d-glucose transport system was sodium-dependent, phlo ... | 2004 | 15630546 |
| calcium handling in sparus auratus: effects of water and dietary calcium levels on mineral composition, cortisol and pthrp levels. | juvenile gilthead sea bream (sparus auratus l.; 10-40 g body mass) were acclimatized in the laboratory to full strength (34 per thousand) or dilute (2.5 per thousand) seawater and fed normal, calcium-sufficient or calcium-deficient diet for nine weeks. mean growth rate, whole-body calcium and phosphorus content and accumulation rates were determined, as well as plasma levels of ionic and total calcium, cortisol and parathyroid hormone related protein (pthrp; a hypercalcemic hormone in fish). whe ... | 2004 | 15498953 |
| organochlorine contamination in some marketable fish in egypt. | ten organochlorine compounds were analyzed in four species of fish (bouri mugil spp., denis sparus auratus, moza boops boops and mousa pegusa lascaris), from four egyptian governorates (south sinai, suez, port-said and demietta). although all fish studied are bottom or near-bottom feeders, remarkable variations between studied species were noticed. the grand total values recorded at the studied governorates were in descending order: bouri>denis>moza>mousa. the highest concentration of pesticides ... | 2004 | 14659941 |
| accumulation of ivermectin in the brain of sea bream, sparus aurata after intraperitoneal administration. | ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. this paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, sparus aurata and accumulate in the brain. the concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive elisa commercial kit. our results showed a rapid u ... | 2004 | 21782707 |
| vibrio ponticus sp. nov., a neighbour of v fluvialis-v. furnissii clade, isolated from gilthead sea bream, mussels and seawater. | a new vibrio species, vibrio ponticus, is proposed to accommodate four marine bacteria isolated from sea water, mussels and diseased sea bream (sparus aurata), at the mediterranean coast of spain. strains are gram negative, slightly halophilic bacteria that require na+ ion for growth, oxidase and catalase positive, negative for arginine dihydrolase and ornithine decarboxylase but positive for lysine decarboxylase and indole, and utilize beta-hydroxybutyrate as a sole carbon source. phylogenetic ... | 2004 | 15490554 |
| comparative toxic effects of formulated simazine on vibrio fischeri and gilthead seabream (sparus aurata l.) larvae. | the use of early life stage (els) tests is a useful tool in risk assessment. the purpose of this study was to compare the sensitivity of the seabream (sparus aurata) larvae with the extensively used microtox test on a commercial formulation containing simazine, an s-triazine herbicide. to this end, survival, growth and histopathological changes displayed by seabream yolk sac larvae exposed during 72 h post-hatching to nominal concentrations of the commercial preparation up to its saturating conc ... | 2004 | 15519419 |
| genomic structure and functional analysis of promoter region of somatolactin gene of sea bream (sparus aurata). | somatolactin (sl) is a pituitary hormone belonging to the growth hormone-prolactin family and is produced in the intermediate lobe of teleosts. the sl gene was isolated from a sea bream genomic library and found to be composed of 5 exons distributed within a 9-kb length of dna. sequence analysis of the proximal promoter region showed the presence of a classical tata box located 59 bp upstream from the initial start atg codon, 5 consensus sequences corresponding to the pit-1 binding element, and ... | 2004 | 15549652 |
| production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-i in european sea bass (dicentrarchus labrax). | a specific radioimmunoassay (ria) for european sea bass (dicentrarchus labrax) growth hormone (gh) was developed and validated. for this purpose, a stable source of gh was produced by means of recombinant dna technology in a bacteria system. the identity of the purified protein (ion exchange chromatography) was demonstrated by western blot and a specific gh antiserum was raised in rabbit. in western blot and ria system, this antiserum recognized specifically native and recombinant gh, and it did ... | 2004 | 15560873 |
| transcriptional regulation of glucose-6-phosphatase catalytic subunit promoter by insulin and glucose in the carnivorous fish, sparus aurata. | increase in glucose-6-phosphatase catalytic subunit (g6pase, g6pc) transcription enhances hepatic glucose production in non-insulin-dependent diabetes mellitus (niddm). the fact that carnivorous fish is an alternative model to study niddm led us to clone and characterise the first g6pc promoter region reported for fish and non-mammalian animals. the 5'-flanking region of g6pc from gilthead sea bream (sparus aurata) was isolated by chromosome walking. with smart race-pcr, the transcription start ... | 2004 | 15591035 |
| production and mechanism of secretion of interleukin-1beta from the marine fish gilthead seabream. | mammalian interleukin-1beta (il-1beta) is a secretory cytokine lacking a signal peptide, which does not follow the classical endoplasmic reticulum to golgi pathway of secretion. its post-translational processing by il-1beta-converting enzyme (ice) and subsequent release from activated macrophages requires atp acting on p2x7 receptors. little information is available on the production and release of fish il-1beta, but the il-1beta gene sequences reported to date lack a conserved ice recognition s ... | 2004 | 14642889 |
| immune response of gilt-head seabream (sparus aurata) to antigens from photobacterium damselae subsp. piscicida. | 2004 | 14675834 | |
| intraspecific diversity of the marine fish pathogen tenacibaculum maritimum as determined by randomly amplified polymorphic dna-pcr. | the aim of the present study was to evaluate the intraspecific genetic variability within tenacibaculum maritimum strains isolated from different species of marine fish. | 2004 | 15012827 |
| acidophilic granulocytes of the marine fish gilthead seabream (sparus aurata l.) produce interleukin-1beta following infection with vibrio anguillarum. | the fish immune response to gram-negative bacteria is poorly understood. in this study, we use a monoclonal antibody (mab) specific to acidophilic granulocytes from the marine fish gilthead seabream (sparus aurata l.), together with an antiserum specific to interleukin-1beta (il-1beta) from this species, in order to investigate whether these cells are involved in the immune response against the pathogenic bacterium vibrio anguillarum and, in particular, in the production of the pro-inflammatory ... | 2004 | 15045578 |
| high resolution crystal structures of piscine transthyretin reveal different binding modes for triiodothyronine and thyroxine. | transthyretin (ttr) is an extracellular transport protein involved in the distribution of thyroid hormones and vitamin a. so far, ttr has only been found in vertebrates, of which piscine ttr displays the lowest sequence identity with human ttr (47%). human and piscine ttr bind both thyroid hormones 3,5,3'-triiodo-l-thyronine (t(3)) and 3,5,3',5'-tetraiodo-l-thyronine (thyroxine, t(4)). human ttr has higher affinity for t(4) than t(3), whereas the reverse holds for piscine ttr. x-ray structures o ... | 2004 | 15082720 |
| effects of high doses of cortisol on innate cellular immune response of seabream (sparus aurata l.). | the effects of high doses of cortisol, biologically the most active corticosteroid in the circulating blood of teleost fish, on gilthead seabream (sparus aurata l.) leucocytes were determined. leucocytes isolated from the head-kidney (the principal haemopoietic organ of this fish species) were incubated with five different concentrations (ranging from 0 to 10(-1) mm) of cortisol for 30, 120, 240, 360, and 480 min and their effects on leucocyte viability and some of the main innate cellular immun ... | 2004 | 15094339 |
| activation of the nitric oxide response in gilthead seabream after experimental infection with photobacterium damselae subsp. piscicida. | inoculation of small gilthead seabream (sparus aurata) (30-75 g body weight) with a sublethal dose of different photobacterium damselae subsp. piscicida (pdp) strains (di-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (no) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. in contrast, no such response was detected in larger fish (150-600 g). since the virulence of pdp correlates with the presence of a polysaccharide capsular layer whi ... | 2004 | 15110332 |
| glucan receptor but not mannose receptor is involved in the phagocytosis of saccharomyces cerevisiae by seabream (sparus aurata l.) blood leucocytes. | 2004 | 15123311 | |
| the effect of dietary administration of the fungus mucor circinelloides on non-specific immune responses of gilthead seabream. | the immunostimulant potential of the whole fungus mucor circinelloides administered in the diet to gilthead seabream (sparus aurata l.) was studied. three different lyophilised strains were used: the wild-type (r7b) and two mutant strains in the carotene-production pathway which are enriched in lycopene (mu224) or beta-carotene (t31). fish were fed diets containing 0 (control) or 10 g of one of the whole m. circinelloides strains per kg feed. after 2, 4 or 6 weeks of treatment the growth rate as ... | 2004 | 15123327 |
| developmental expression of three forms of gonadotropin-releasing hormone and ontogeny of the hypothalamic-pituitary-gonadal axis in gilthead seabream (sparus aurata). | to address the complexity of the origin of the gnrh system in perciforms, we investigated the ontogenic expression of three gnrhs in gilthead seabream. using in situ hybridization, chicken (c) gnrh-ii mrna-expressing cells were detected in the hindbrain at 1.5 days postfertilization (dpf) and in the midbrain at 2 dpf and thereafter; the hindbrain signals became undetectable after 10 dpf. salmon (s) gnrh mrna-expressing cells were first seen in the olfactory placode at 3 dpf, started caudal migra ... | 2004 | 15163612 |
| the tumor necrosis factor alpha of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner. | information on the bioactivities of non-mammalian cytokines is scant due to the lack of the recombinant molecules and specific antibodies. we produced the mature predicted peptide of tumor necrosis factor alpha (tnf alpha) from the bony fish gilthead seabream (sparus aurata l.) (sbtnf alpha), and its biological role was determined in vitro and in vivo. we first demonstrated by analytical size-exclusion chromatography that sbtnf alpha is an oligomeric protein but the dimer appears to predominate ... | 2004 | 15170511 |
| protein and glycoprotein content of lymphocystis disease virus (lcdv). | the polypeptide and glycoprotein composition of eight strains of the fish-pathogenic lymphocystis disease virus (lcdv) isolated from gilt-head seabream (sparus aurata), blackspot seabream (pagellus bogaraveo), and sole (solea senegalensis) were determined. the protein electrophoretic patterns of all lcdv isolates were quite similar regardless of the host fish, showing two major proteins (79.9 and 55.6 kda) and a variable number of minor proteins. three groups of lcdv isolates were distinguished ... | 2004 | 15248160 |
| potential drug (oxytetracycline and oxolinic acid) pollution from mediterranean sparid fish farms. | the potential for input of two common antibacterial agents in mediterranean fish farms, oxytetracycline (otc) and oxolinic acid (oa), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, sparus aurata and sharpsnout sea bream diplodus puntazzo. oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of otc was found to be considerably lower in both species ( ... | 2004 | 15276333 |
| nodavirus infections in israeli mariculture. | viral encephalopathy and retinopathy (ver) infections were diagnosed in five fish species: epinephelus aeneus, dicentrarchus labrax, sciaenops ocellatus, lates calcarifer and mugil cephalus cultured on both the red sea and mediterranean coasts of israel during 1998-2002. spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. with transmission electron microscopy, paracrystalline arrays and pieces of membrane-associated non-enveloped virions measur ... | 2004 | 15291788 |
| maternal transfer of humoral specific and non-specific immune parameters to sea bream (sparus aurata) larvae. | immunisation of sea bream (sparus aurata l.) broodstock with a novel vaccine mixture of photobacterium damsela subsp. piscicida sk7 (phdp) was performed during the period of egg development and the changes in specific and non-specific humoral immune parameters were measured. total immunoglobulin level, specific antibody titre, anti-protease activity and lysozyme activity were significantly higher in immunised parents compared to the control. after spawning significantly higher anti-protease acti ... | 2004 | 15313509 |
| toxicity of a commercial herbicide containing terbutryn and triasulfuron to seabream (sparus aurata l.) larvae: a comparison with the microtox test. | the present study aimed to compare the sensitivity of larvae of the gilthead seabream (sparus aurata), a valuable fish species of the spanish south atlantic littoral, with the extensively used microtox test on a commercial herbicide formulation containing terbutryn (59.4%) and triasulfuron (0.6%). to this purpose, mortality displayed by endogenous feeding s. aurata larvae exposed during 72 h post-hatching to nominal concentrations of the commercial formulation and bioluminescence of the marine b ... | 2004 | 15327877 |
| total serum immunoglobulin m levels are affected by immunomodulators in seabream (sparus aurata l.). | immunoglobulin m (igm) is a major component of the teleost humoral immune system. despite the significance of igm levels as an immune parameter, there are relatively few studies on changes induced in its total levels in serum. this study examines the effects of several immunomodulators (vitamin a, chitin, yeast cells or levamisole, which act as immunostimulants, and crowding, hypoxia or anaesthetics, which act as stressors) upon the total serum igm levels of non-immunized gilthead seabream (spar ... | 2004 | 15350750 |
| characterization and comparison of fatty acyl delta6 desaturase cdnas from freshwater and marine teleost fish species. | fish are the most important dietary source of the n-3 highly unsaturated fatty acids (hufa), eicosapentaenoic (epa) and docosahexaenoic acid (dha), that have particularly important roles in human nutrition reflecting their roles in critical physiological processes. the objective of the study described here was to clone, functionally characterize and compare expressed fatty acid desaturase genes involved in the production of epa and dha in freshwater and marine teleost fish species. putative fatt ... | 2004 | 15465674 |
| identification of a fasl-like molecule in leucocytes of the teleost fish gilthead seabream (sparus aurata l.). | the possible expression of fasl in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse fasl monoclonal antibody. the results pointed to a cytosolic fasl-like, but not a membrane-like form, in resting leucocytes from head-kidney, thymus, spleen, blood and peritoneal exudate. immunoblotting revealed a 19kda band in resting leucocytes, while activated leucocytes showed the same band and another of 39kda. the fasl-like molecule is i ... | 2003 | 12477498 |
| localization of igf-i, igf-i receptor, and igfbp-2 in developing umbrina cirrosa (pisces: osteichthyes). | in this study, the distribution of igf-i, igf type i receptor (igf-ir), and igf-binding protein 2 (igfbp-2) was investigated during larval and post-larval developmental stages of the shi drum (umbrina cirrosa) by immunohistochemistry using antisera raised against sparus aurata igf-i and igf-ir, and against mouse igfbp-2. immunoreactivity of the mitogenic marker pcna (proliferating cell nuclear antigen) was used for assessment of cellular proliferation. distribution of igf-i mrna was studied by i ... | 2003 | 12606266 |
| matrix gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish sparus aurata. | matrix gla protein (mgp) is a member of the family of extracellular mineral-binding gla proteins, expressed in several tissues with high accumulation in bone and cartilage. although the precise molecular mechanism of action of this protein remains unknown, all available evidence indicates that mgp plays a role as an inhibitor of mineralization. we investigated the sites of gene expression and protein accumulation of mgp throughout development of the bony fish sparus aurata, by in situ hybridizat ... | 2003 | 12667547 |
| phagocytosis and peroxidase release by seabream (sparus aurata l.) leucocytes in response to yeast cells. | a flow cytometric method was adapted to evaluate phagocytosis by gilthead seabream leucocytes after incubation with yeast cells (saccharomyces cerevisiae). head-kidney leucocytes were incubated in vitro for different times in different proportions with heat-killed fluorescein isothiocyanate (fitc)-labeled yeast cells to study the kinetics of phagocytosis. attached and internalized yeast cells were differentiated by quenching fitc-labeled s. cerevisiae with trypan blue dye. only internalized cell ... | 2003 | 12704699 |
| a mannose-receptor is possibly involved in the phagocytosis of saccharomyces cerevisiae by seabream (sparus aurata l.) leucocytes. | in this paper the possible involvement of the mannose-receptor on the non-specific recognition and phagocytosis of heat killed yeast cells (saccharomyces cerevisiae) by gilthead seabream (sparus aurata l.) head-kidney leucocytes was established by studying the ability of different sugars to inhibit the uptake of the yeast cells by leucocytes. leucocytes were preincubated for 30min with different concentrations of sugar (alpha-mannan, d-mannose, d-fucose, l-fucose, d-glucose, d-glucosamine and n- ... | 2003 | 12711272 |
| pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream sparus aurata. | the present study investigated the kinetic profile of flumequine (flu) in gilthead sea bream sparus aurata (170 g) held at 19 degrees c and evaluated its in vitro efficacy against important bacterial diseases in mediterranean mariculture. following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. tissue penetration of flu was low, since both t ... | 2003 | 12718468 |
| carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms. | a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ... | 2003 | 12747637 |
| existence of two o-serotypes in the fish pathogen pseudomonas anguilliseptica. | the serological characteristics of a group of 32 pseudomonas anguilliseptica strains isolated in spain from seabream (sparus aurata) and turbot (scophthalmus maximus) were compared with a total of 18 collection strains of this bacterial species with different geographical and host origin. the employment of different techniques, including slide agglutination, microagglutination and dot blot, allowed us to establish two serological groups, one comprising practically all the eel isolates, and the o ... | 2003 | 12829386 |
| virulence and molecular typing of vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and european sea bass. | vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax) cultured in several fish farms located on the spanish mediterranean coast. the prevalence of the bacterium was significantly higher in european sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (june to november). after phenotypic char ... | 2003 | 12866856 |
| determination of tissue and plasma concentrations of pthrp in fish: development and validation of a radioimmunoassay using a teleost 1-34 n-terminal peptide. | a specific and sensitive radioimmunoassay (ria) for the n-terminus of sea bream (sparus auratus) and flounder (platichthys flesus) parathyroid hormone-related protein (pthrp) was developed. a (1-34) amino-terminal sequence of flounder pthrp was synthesized commercially and used as the antigen to generate specific antiserum. the same sequence with an added tyrosine (1-35(tyr)) was used for iodination. human (1-34) parathyroid hormone (pth), human (1-34) pthrp, and rat (1-34) pthrp did not cross-r ... | 2003 | 12899855 |
| assessment of dna vaccine potential for gilthead sea bream (sparus aurata) by intramuscular injection of a reporter gene. | naked circular plasmid dna containing the cytomegalovirus (cmv)-promoter-driven lacz reporter gene (pcmv-lacz) was injected in the epaxial muscle of gilthead sea bream (sparus aurata). a mosaic pattern of expression of beta-galactosidase (beta-gal) in the myofibres at the site of injection was visualised by in situ histochemical staining using 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside. as measured by o-nitrophenyl-beta-d-galactopyranoside assay, beta-gal enzymatic activity was found to ... | 2003 | 12969650 |
| fsh-, lh-, and tsh-expressing cells during development of sparus aurata l. (teleostei). an immunocytochemical study. | follicle-stimulating hormone-like gonadotropin (fsh), luteinizing hormone-like gonadotropin (lh), and thyrotropin (tsh) cells were detected in adult and developing pituitary gland of gilthead seabream. antisera obtained against the alpha- and beta-subunits of fsh (anti-my fsh) and the beta-subunit of lh (anti-my lhbeta), respectively, of the teleost mediterranean yellowtail, and an antiserum against the beta-subunit of human tsh (anti-h tshbeta), were applied to identify and follow these cells d ... | 2003 | 13129505 |
| distribution and potential role of cytosolic water-soluble phosphodiesters in fish. | the distribution of water-soluble phosphodiesters (wspdes) visible by nuclear magnetic resonance (nmr) in some intact tissues of rainbow trout (oncorhynchus mykiss walbaum) and in perchloric extracts after partial purification was examined by (31)p nmr spectroscopy. the compounds of interest were serine ethanolamine phosphate (sep), threonine ethanolamine phosphate (tep), glycerophosphorylcholine (gpc), and glycerophosphorylethanolamine (gpe). tep and sep were mostly accumulated in the heart and ... | 2003 | 13130434 |
| immunostimulant properties of a cell wall-modified whole saccharomyces cerevisiae strain administered by diet to seabream (sparus aurata l.). | the possible differences in the immunostimulant effects between whole wild saccharomyces cerevisiae and a mutant strain (fks-1) administered in the diet to gilthead seabream (sparus aurata l.) were studied. fish were fed diets containing 0 (control) or 10 g whole wild yeast or fks-1 strain per kilogram feed for 2, 4 or 6 weeks. after each sampling, the innate humoral (complement, lysozyme and peroxidases) and cellular (intracellular peroxidases, phagocytosis, respiratory burst activity and cytot ... | 2003 | 14592731 |
| experimental transmission of cryptosporidium molnari (apicomplexa: coccidia) to gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.). | cryptosporidium molnari was experimentally transmitted to gilthead sea bream (sparus aurata) and european sea bass (dicentrachus labrax) by oral infection with infected stomach scrapings. the infection was also cross-transmitted from infected gilthead sea bream to sea bass by cohabitation. the course of the infection was assessed after necropsy by three microscopic diagnostic methods and their sensitivity was compared. at the end of all the experiments the prevalence of infection reached 100%. i ... | 2003 | 12923629 |
| thynnascaris rhacodes sp. n. (nematoda: ascaridoidea) in fishes from the israeli mediterranean coast. | thynnascaris rhacodes sp. n. infects pelates quadrilineatus, solea vulgaris, boops boops, lithognathus mormyrus, obleida melanura, diplodus vulgaris, d. sargus, and sparus auratus from the eastern mediterranean sea. unique in that the cuticle on the female tail is wrinkled, the ascaridoid is further characterized by possessing 27 to 35 precloacal papillae, a body length of 11 to 85 mm, similar spicules 3 to 6% of body length, a length-ratio of intestinal cecum to ventricular appendage of 1 : 1 t ... | 2003 | 570370 |
| molecular cloning and characterization of gilthead sea bream (sparus aurata) growth hormone receptor (ghr). assessment of alternative splicing. | the full-length growth hormone receptor (ghr) of gilthead sea bream (sparus aurata) was cloned and sequenced by rt-pcr and rapid amplification of 5'and 3'ends. the open reading frame codes for a mature 609 amino acid protein with a hydrophobic transmembrane region and all the characteristic motifs of ghrs. sequence analysis revealed a 96 and 76% of amino acid identity with black sea bream (acanthopagrus schlegeli) and turbot (scophthalmus maximus) ghrs, respectively, but this amino acid identity ... | 2003 | 12941635 |
| temperature and the expression of seven muscle-specific protein genes during embryogenesis in the atlantic cod gadus morhua l. | seven cdna clones coding for different muscle-specific proteins (msps) were isolated from the fast muscle tissue of atlantic cod gadus morhua l. in situ hybridization using crna probes was used to characterize the temporal and spatial patterns of gene expression with respect to somite stage in embryos incubated at 4 degrees c, 7 degrees c and 10 degrees c. myod transcripts were first observed in the presomitic mesoderm prior to somite formation, and in the lateral compartment of the forming somi ... | 2003 | 12909700 |
| annual variation of complement, lysozyme and haemagglutinin levels in serum of the gilthead sea bream sparus aurata. | 2003 | 14550674 | |
| biochemical changes during post-larval growth in white muscle of gilthead sea bream (sparus aurata) fed zinc-fortified diets. | 2003 | 14535393 | |
| apoptosis in sea bream sparus aurata eggs. | in the present study, the sea-bream sparus aurata, a pelagic egg spawner, was used as experimental model, in order to establish the occurrence of apoptosis in vertebrates with external reproduction. the same female ovulates floating and nonfloating eggs, but only the former, after fertilization, proceed to embryo development. the eggs were divided into floating and nonfloating and both were analyzed for the presence of several apoptosis markers. the results here reported provide evidence that th ... | 2003 | 14502608 |
| isolation of sparus auratus prolactin gene and activity of the cis-acting regulatory elements. | a sea bream prolactin (sbprl) gene was isolated using a prolactin cdna fragment, generated by pcr as a probe. the gene analyzed comprises 3.5 kb of dna containing five exons as described previously for other fish prl genes. analysis of 1.0 kb of the proximal promoter sequence reveals a consensus tataa box, up to seven (a/t)3ncat consensus motifs for binding of the pituitary-specific factor pit-1 and putative creb and gata binding sites. cho culture cells co-transfected with a sbprl promoter sequ ... | 2003 | 13129503 |
| expression and cellular localization of insulin-like growth factor-ii protein and mrna in sparus aurata during development. | the spatial localization of igf-ii protein and mrna was investigated during larval and postlarval developmental stages of the gilthead sea bream (sparus aurata) by immunohistochemistry and in situ hybridization, using specific antisera and riboprobes. steady-state levels of igf-ii mrna in larvae were determined by northern blot analysis and were found to be increased. immunoreactivity towards igf-ii was found in larval skin, muscle, gills, gut, olfactory epithelium and kidney. after metamorphosi ... | 2003 | 12904176 |
| growth hormone expression in ontogenic development in gilthead sea bream. | the pattern of expression of the growth hormone (gh) gene was studied during the early development of gilthead sea bream ( sparus aurata). the gh transcript was detected from the 2nd day of the larval stage onwards. in the next stages the expression level fluctuated, possibly due to different regulatory factors. the distribution of gh mrna studied by in situ hybridization (ish) was found to be pituitary specific. hybridization signals for gh mrna were detected for the first time in 4-day-old lar ... | 2003 | 12827495 |