Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| gonadotropin alpha-subunit glycoprotein from channel catfish (ictalurus punctatus) and its expression during hormone-induced ovulation. | complementary dna (cdna) encoding the channel catfish (ictalurus punctatus) gonadotropin (gth) alpha-subunit glycoprotein was cloned by polymerase chain reaction (pcr) from a plasmid library made from pituitary rna. complete cdna cloning was achieved by carrying out two pcr reactions: one with an upstream sense primer plus the universal sequencing primer, located downstream of the poly(a) sequence of the cdna in the plasmid vector, to amplify the downstream portion of the cdna; the other with a ... | 1997 | 9284560 |
| characterization of hepatic metallothionein expression in channel catfish (ictalurus punctatus) by reverse transcriptase polymerase chain reaction. | measurements of metallothioneins (mts) can be labour intensive and require expensive probes and or analytical instrumentation. in contrast, the reverse transcriptase polymerase chain reaction (rt-pcr0 has provided a relatively sensitive and simple mechanism to measure expression of mt in sentinel species in aquatic environments. the objectives of this study were to characterize expression of hepatic mt in a commonly occurring fish species of the southeastern united states, the channel catfish (i ... | 1997 | 23899176 |
| production of amylase by the intestinal microflora in cultured freshwater fish. | the amylase-producing ability of the intestinal microflora in cultured specimens of ayu, carp, channel catfish, japanese eel and tilapia was determined. mean viable counts of aerobes and anaerobes ranged from 1.1 x 10(6) to 3.7 x 10(8) cfu g-1 and from 1.3 x 10(3) to 1.6 x 10(8) cfu g-1, respectively. aeromonas spp. and bacteroidaceae were predominant in four to five fish species. of 206 strains examined, 65 (31.6%) produced > or = 0.01 u amylase ml-1. the percentage of producers differed among ... | 1997 | 9081303 |
| multiple excitatory receptor types on individual olfactory neurons: implications for coding of mixtures in the spiny lobster. | the aim of our paper was to investigate whether single olfactory receptor neurons (orns) of the spiny lobster panulirus argus functionally express more than one type of receptor, examine the consequences of this on coding of mixtures, and compare principles of odorant mixture coding by spiny lobsters with that by the channel catfish, which has been studied extensively using the same experimental and analytical procedures (caprio et al. 1989; kang and caprio 1991). we examined responses of indivi ... | 1997 | 9163926 |
| functional motifs in the igh enhancer of the channel catfish. | the transcriptional enhancer (emu3') within the ig heavy chain (igh) locus of the channel catfish differs from those found in mammalian igh loci in both its location and structure. however, upon transfection into fish or mouse lymphocytes, it activates transcription to an extent equivalent to that of the mouse igh intronic enhancer (emu). potential transcription factor binding motifs in emu3' are more numerous than in mammalian igh enhancers, and are dispersed over 1.6 kilobases. we transfected ... | 1997 | 9211744 |
| atypical angiotensin ii receptors coupled to phosphoinositide turnover/calcium signalling in catfish hepatocytes. | in catfish (ictalurus punctatus) hepatocytes angiotensin ii induced an immediate increase in cytosolic ca2+ concentration. other angiotensin analogues also induced this effect including: human angiotensin ii, fish angiotensin ii, human angiotensin iii, human angiotensin i, fish angiotensin i and saralasin. cgp 42112a induced a very small effect at the highest concentration tested and angiotensin iv was without effect. angiotensin ii also increased the resynthesis of phosphatidylinositol and the ... | 1997 | 9223623 |
| immunization with viral antigens: viral diseases of carp and catfish. | the viral diseases of carp and catfish for which vaccines have been produced are spring viraemia of carp (svc), grass carp haemorrhage disease (gchd) and channel catfish virus disease (ccvd). field trials of a commercially produced injectable vaccine conducted over several years have shown that carp can be protected against svc. however the supporting data were predominantly qualitative rather than quantitative. large-scale field trials of an experimental oral attenuated vaccine against svc viru ... | 1997 | 9270851 |
| comparison of whole-cell antigens of pressure- and formalin-killed flexibacter columnaris from channel catfish (ictalurus punctatus). | to identify and compare immunodominant antigens in whole-cell lysates of pressure- and formalin-killed flexibacter columnaris. | 1997 | 9285002 |
| parapinopsin, a novel catfish opsin localized to the parapineal organ, defines a new gene family. | multiple sites of extraretinal photoreception are present in vertebrates, but the molecular basis of extraretinal phototransduction is poorly understood. this study reports the cloning of the first opsin specifically expressed in the directly photosensitive pineal and parapineal of cold-blooded vertebrates. this opsin, identified in channel catfish and termed parapinopsin, defines a new gene family of vertebrate photopigments and is expressed in a majority of parapinealocytes and a subset of pin ... | 1997 | 9334384 |
| methylmercury in fish from owyhee reservoir in southeast oregon: scientific uncertainty and fish advisories. | data collected during 1987-1994 showed elevated levels of mercury (hg) in fish tissue from the owyhee reservoir in southeastern oregon. sixty-five percent of the samples analyzed had total hg levels exceeding the us environmental protection agency's (epa) health screening value of 0.6 mg/kg. eighteen out of 89 (20%) fish tissue samples also had total hg levels greater than the us food and drug administration's (fda) mercury action level of 1.0 mg/kg. the overall mean hg content for all fish coll ... | 1997 | 9335158 |
| quantification of immunoglobulin in the serum and mucus of channel catfish at different ages and following infection with edwardsiella ictaluri. | an enzyme-linked immunosorbent assay was developed to quantify immunoglobulin (ig) in the serum and mucus of channel catfish (ictalurus punctatus). the capture antibody (ab) was a monoclonal ab to channel catfish ig and the detector was a polyclonal goat serum against channel catfish ig. detectable levels of channel catfish ig were 0.1 microgram ml-1 and coefficients of intra- and interassay variations were 20% and 15%, respectively. serum ig concentration was found to increase from 0.36 +/- 0.2 ... | 1997 | 9336885 |
| killing of edwardsiella ictaluri by macrophages from channel catfish immune and susceptible to enteric septicemia of catfish. | the role of peritoneal macrophages in immunity to enteric septicemia of catfish (esc) after infection with live edwardsiella ictaluri was investigated. channel catfish macrophage-mediated bacteriocidal activity was dependent on the macrophage:bacteria ratio. ratios of 1:1 to 1:12 exhibited significant differences (p < or = 0.05) in killing between macrophages from immune fish when compared to killing by macrophages from susceptible fish at 2.5 h. at 5 h, macrophages from immune fish were capable ... | 1997 | 9336886 |
| attenuation, persistence, and vaccine potential of an edwardsiella ictaluri pura mutant. | in this study, an adenine-auxotrophic strain of edwardsiella ictaluri was constructed and its virulence, tissue persistence, and vaccine efficacy were evaluated. a clone containing the pura gene was isolated from an e. ictaluri genomic library, sequenced, and shown to have an overall sequence identity of 79.3% at the nucleotide level and 85.7% at the amino acid level with the escherichia coli pura gene. the cloned e. ictaluri pura gene was mutated by deleting a 598-bp segment of the gene and ins ... | 1997 | 9353045 |
| molecular biology of vertebrate transcription factor iiia: cloning and characterization of tfiiia from channel catfish oocytes. | tfiiia regulates 5s rrna synthesis and is the prototype of the cys2his2 superfamily of zinc finger proteins. because the tfiiia aa sequence is highly diverged, elucidating species variation in this factor will yield insights into how zinc fingers bind dna and how this protein regulates rnapiii transcription. this study reports the identification, cloning and functional divergence of oocyte tfiiia from the channel catfish. catfish oocyte tfiiia was identified by its association with 5s rrna in im ... | 1997 | 9426240 |
| anti-viral cytotoxic cells in the channel catfish (ictalurus punctatus). | cytotoxic cells isolated from the head kidney and peripheral blood of the channel catfish appear to represent distinct subpopulations of effector cells. previous studies showed that the former lyse xenogeneic natural killer (nk) cell targets, whereas the latter preferentially lyse allogeneic cells. here we extend these studies and present data suggesting a third class of cytotoxic effectors responsible for killing virus-infected allogeneic and autologous cells. peripheral blood leukocytes (pbls) ... | 1996 | 8799617 |
| kinetic analysis of the in vitro inhibition, aging, and reactivation of brain acetylcholinesterase from rat and channel catfish by paraoxon and chlorpyrifos-oxon. | in rats, the phosphorothionate insecticide parathion exhibits greater toxicity than chlorpyrifos, while in catfish the toxicities are reversed. the in vitro inhibition of brain acetylcholinesterase (ache) by the active metabolites of the insecticides and the rates at which these inhibitor-enzyme complexes undergo reactivation/ aging were investigated in both species. rat ache was more sensitive to inhibition than catfish ache as demonstrated by greater bimolecular rate constants (ki) in rats tha ... | 1996 | 8806854 |
| g alpha 9/g alpha 11: immunolocalization in the olfactory epithelium of the rat (rattus rattus) and the channel catfish (ictalurus punctatus). | the immunohistochemical localization of g alpha 9/g alpha 11 was studied in the olfactory and respiratory epithelium of two representative vertebrates, the rat and the channel catfish. localization in the rat was found at the apical surface of cells in the epithelium and within nerve tracts in the lamina propria. immunostaining of neuronal cilia and supporting cell microvilli was confirmed by electron microscopy. immunoreactivity on the ipsilateral neuroepithelium was abolished five weeks follow ... | 1996 | 8843091 |
| polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus. | polymerase chain reaction (pcr) amplification assays were developed for the detection of thymidine kinase (tk) and protein kinase (pk)-related genes of the channel catfish virus (ccv). two pairs of primers were constructed based on the published nucleotide sequences of ccv and were used to amplify the expected fragments of 584 and 755 bp for the tk and pk-related genes, respectively. the amplified fragments were shown to be specific for each of the target genes by chemiluminescence southern blot ... | 1996 | 8882938 |
| humoral immunoglobulins of the white sturgeon, acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies. | white sturgeon (acipenser transmontanus) immunoglobulin (ig) was purified from serum by two methods, ion-exchange chromatography and gel filtration and precipitation of the euglobulin fraction. the purity of these immunoglobulin preparations was confirmed by gel electrophoresis. sequence analysis of the n-terminal amino acids confirmed that the purified protein was immunoglobulin. the major portion of the immunoglobulin preparation consisted of two proteins with estimated molecular weights (m.w. ... | 1996 | 8915630 |
| one-step immunoaffinity purification and partial characterization of hypophyseal growth hormone from the african catfish, clarias gariepinus (burchell). | growth hormone (gh) was purified from african catfish (clarias gariepinus) pituitary extracts in a single step by use of immunoaffinity chromatography. a monoclonal antibody to chicken gh, which labels the catfish hypophyseal somatotropes in immunocytochemistry, was coupled to cnbr-activated sepharose, and crude alkaline pituitary extracts were run over the immunoadsorbent. reversed-phase high-performance liquid chromatography analysis of the eluted material suggested heterogeneity, whereas silv ... | 1996 | 8925443 |
| passive immunization of channel catfish (ictalurus punctatus) against the ciliated protozoan parasite ichthyophthirius multifiliis by use of murine monoclonal antibodies. | fish acquire immunity against the ciliated protozoan parasite ichthyophthirius multifiliis following sublethal infection. the immune response includes the elaboration of humoral antibodies against a class of abundant surface membrane proteins referred to as immobilization antigens (i-antigens). antibodies against these proteins immobilize the parasite in vitro, suggesting a potential role for the i-antigens in protective immunity. to test this hypothesis, passive immunization experiments were ca ... | 1996 | 8926073 |
| intestinal coccidiosis of channel catfish, ictalurus punctatus. | 1996 | 8953547 | |
| the capsid architecture of channel catfish virus, an evolutionarily distant herpesvirus, is largely conserved in the absence of discernible sequence homology with herpes simplex virus. | although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. the capsid shell is icosahedrally symmetric (t = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (hsv-1). channel catfish virus (ccv) has the gross morphology of a herpesvirus, although no relationship to other herpesviruses is evident from the sequences of its proteins. to examine ccv capsid architectu ... | 1996 | 8560760 |
| the attenuated v60 strain of channel catfish virus possesses a deletion in orf50 coding for a potentially secreted glycoprotein. | a wild-type strain of channel catfish virus was compared at the genomic level with the attenuated strain v60. in addition to several minor differences, restriction mapping revealed one major deletion (approximately 1200 bp) in orf50 of the v60 strain. cloning and sequencing of part of this orf confirmed the presence of a 1164-bp deletion. it should result in a protein of 282 amino acids instead of 670. the predicted truncated protein lacks most of a threonine-rich, highly repetitive region in it ... | 1996 | 8610472 |
| connections of octaval and lateral line nuclei of the medulla in the goldfish, including the cytoarchitecture of the secondary octaval population in goldfish and catfish. | cytoarchitectural analyses combined with injections of the tracer horseradish peroxidase in various structures in the brain of the goldfish, carassius auratus, have defined some of the major components of acoustic and lateral line mechanosensory circuits between the medulla and midbrain. the main acoustic receptor in carassius, the saccule, is known to provide a major input to the dorsomedial zone of the descending octaval nucleus. the dorsomedial zone in turn projects bilaterally to the seconda ... | 1996 | 8680846 |
| neutrophilic granulocytes in carp, cyprinus carpio, possess a spontaneous cytotoxic activity. | this study demonstrates for the first time that carp (cyprinus carpio) neutrophilic granulocytes from the head kidney possess potent spontaneous cytotoxic activity against several human tumor cell lines. carp head kidney cells isolated at a density of 1.09 g/ml contained more than 90% neutrophilic granulocytes. these cells were round and approximately 10 millimicrons in diameter with reniform or polymorphic nuclei and slightly eosinophilic cytoplasm when stained with giemsa. electron microscopy ... | 1995 | 8617402 |
| use of aquatic organisms as models to determine the in vivo contribution of flavin-containing monooxygenases in xenobiotic biotransformation. | in an attempt to understand the evolution and role of flavin-containing monooxygenases (fmos) in xenobiotic biotransformation by aquatic organisms, a survey of hepatic fmo activity (n,n-dimethylaniline n-oxidase and thiourea oxidase) was performed in one brackish water fish and 17 species of freshwater fish, one of which was anadromous. only hepatic microsomes from the brackish water medaka (oryzia latipes), the freshwater centrarid lepomis macrochirus, and the anadromous rainbow trout (oncorhyn ... | 1995 | 8541983 |
| sustained growth of ichthyophthirius multifiliis at low temperature in the laboratory. | applied and basic research on the ciliate ichthyophthirius multifiliis, an obligate parasite of freshwater fishes, requires passage on fish hosts to maintain laboratory stocks. however, continual repeated passage results in senescence of parasite clones over time. because growth and development are directly correlated to water temperature, our objective was to grow the parasite at low temperature in order to extend the period that the organism remains on the fish, thus reducing: (1) the number o ... | 1995 | 8544046 |
| biochemical mechanisms contributing to species differences in insecticidal toxicity. | comparison of published ld50 or lc50 levels for a variety of insecticides in several vertebrate species indicate that a wide range of toxicity levels exist, and these cannot be easily predicted within either a chemical group or within a species. there is a relatively limited data base documenting interactions between insecticides and other chemicals, either agricultural or non-agricultural; however, the fact that all major insecticide groups perturb nervous system function as their primary mecha ... | 1995 | 8571366 |
| high molecular weight dna from nucleated erythrocytes for use in pulsed-field gel electrophoresis (pfge). | nucleated erythrocytes of lower vertebrates provide a source of genomic dna that can be used in pulsed-field gel electrophoresis (pfge) studies. difficulties reported in the preparation of chicken erythrocyte dna for analysis by pfge suggest that the presence of hemoglobin iron may result in iron-mediated dna degradation. we report here modifications to the procedures established for isolation of high molecular weight dna from mammalian cells. by increasing the volume of buffers and extending th ... | 1995 | 8574892 |
| molecular biology of fish viruses: a review. | the goal of this review is to present some of the recent molecular aspects in the fish virus studies. although more than 50 different fish virus have been isolated and tissue-culture adapted, very few of them have been molecularly cloned and sequenced. five virus families have been mostly studied: birnaviridae, the prototype being the infectious pancreatic necrosis virus (ipnv), and the channel catfish virus (ccv) belonging to the herpesviridae family. in the iridoviridae family, the fish lympho ... | 1995 | 8581005 |
| properties of iridoviruses from ornamental fish. | an iridovirus from 2 species of freshwater tropical imported fish was examined for its antigenic and biochemical properties and virulence for 3 species of food fish. the virus was found in both guppy (poecilia reticulata) and doctor fish (labroides dimidatus). the virus replicated in numerous fish cell lines including bf-2, cco and epc. virus titers reached 10(8) tcid50/ml in each cell line. the virions had similar size and morphology to those previously reported to cause systemic infections amo ... | 1995 | 8581016 |
| antibodies specific for channel catfish virus cross-react with pacific oyster, crassostrea gigas, herpes-like virus. | 1995 | 8581033 | |
| deletion of thymidine kinase gene attenuates channel catfish herpesvirus while maintaining infectivity. | a recombinant thymidine kinase (tk) gene deletion mutant of channel catfish herpesvirus (ccv) was constructed and compared to the parent virus for replicative ability in cell culture, for the ability to infect, replicate, and cause lethal disease in the channel catfish host, and for the ability to induce protective immunity in channel catfish fingerlings. there was no difference between parent ccv and the tk-negative mutant (ccvtk-) in viral production in cell culture. however, in immersion chal ... | 1995 | 7778299 |
| identification of structural proteins of channel catfish virus by mass spectrometry. | emerging methods that couple mass spectrometry of fragments generated from isolated proteins with database searching offer a powerful means of identifying proteins and the genes that encode them. we have applied this technology to a herpesvirus, channel catfish virus, and have identified 12 genes, 11 viral and 1 cellular, that encode 16 principal structural proteins. these proteins include three components of the mature capsid and a potential scaffolding protein present in immature capsids, thre ... | 1995 | 7856078 |
| molecular cloning of a phosphoinositide-specific phospholipase c from catfish olfactory rosettes. | a 2.7 kb clone encoding the partial (about 66%) sequence of a phosphoinositide-specific phospholipase c (plc) was isolated from a cdna library constructed from channel catfish (ictalurus punctatus) olfactory rosettes. the clone, designated 30c7, was completely sequenced by automated dna sequencing and was found to share significant homology with rat and bovine plcs of the delta 1 isotype. in situ hybridization showed that 30c7 transcripts were expressed in a small subpopulation of olfactory neur ... | 1995 | 7476017 |
| contrast gain control in the lower vertebrate retinas. | control of contrast sensitivity was studied in two kinds of retina, that of the channel catfish and that of the kissing gourami. the former preparation is dominantly monochromatic and the latter is bichromatic. various stimuli were used, namely a large field of light, a spot-annulus configuration and two overlapping stimuli of red and green. recordings were made from horizontal, amacrine, and ganglion cells and the results were analyzed by means of wiener's theory, in which the kernels are the c ... | 1995 | 7561745 |
| cloning of a cdna encoding the s13 ribosomal protein from the catfish ictalurus punctatus. | a cdna clone (rps13) encoding the s13 ribosomal protein (rps13) has been isolated from the catfish ictalurus punctatus, and is the first example of an rps13 isolated from a teleost species. the deduced 151-amino-acid (aa) sequence is 96% identical to the rat and human rps13, extending the evolutionary conservation of this protein. | 1995 | 7590288 |
| immediate-early transcription from the channel catfish virus genome: characterization of two immediate-early transcripts. | with cdna probes and by northern (rna) blot analysis, a region containing immediate-early (ie) genes in the channel catfish virus (ccv) genome was identified. ie transcription in ccv-infected cells appears to be restricted to the terminal repeat region, suggesting that ccv is most closely related to the alpha subfamily of herpesviruses. ccv dna fragments from this region encoding ie transcripts were cloned. northern analysis with one of these cloned fragments, a 3,927-bp ecori-xbai fragment, ind ... | 1995 | 7707544 |
| in vitro biotransformation of aflatoxin b1 (afb1) in channel catfish liver. | the biotransformation of the dietary carcinogen aflatoxin b1 (afb1) was examined in hepatic microsomal and cytosolic fractions from channel catfish, an aquatic species shown to be refractory to afb1 toxicity and reported to be resistant to afb1 hepatocarcinogenesis. catfish liver microsomes catalyzed the in vitro oxidation of afb1 to the reactive afb1-8,9-epoxide (afbo) at high substrate concentrations (128 microm afb1) but not at low substrate concentrations (16 microm afb1) which were more rep ... | 1995 | 7747288 |
| immunotoxicity in channel catfish, ictalurus punctatus, following acute exposure to tributyltin. | tributyltin (tbt) is a trialkylated organotin formulated for use primarily as a biocide for aquatic and agricultural industries. although macrophages isolated from toadfish (opsanus tau) are sensitive to exposure, very little is known about the effects of tbt on fish humoral immunity and non-specific cytotoxic cell (ncc) functions. to evaluate the effects of tbt on these parameters, channel catfish (ictalurus punctatus) were given a single intraperitoneal injection of corn oil with 0 (vehicle co ... | 1995 | 7755401 |
| convergence of oral and extraoral information in the superior secondary gustatory nucleus of the channel catfish. | neurons within the superior secondary gustatory nucleus (ngs) of the channel catfish were examined electrophysiologically for responses to mechanical and chemical stimulation of neural peripheral receptive fields (rfs). of the 28 single units sampled, 18 had mechanosensory rfs on the extraoral epithelium, two had rfs within the oropharyngeal cavity, and eight had rfs that included both oral and extraoral surfaces. rf sizes varied from approximately 2 cm2 on the ipsilateral lips and barbels to th ... | 1995 | 1382808 |
| a demonstration of human beta-2-microglobulin specific association with the surface of teleostean cells. | purified human beta-2 microglobulin (beta 2m) and a human beta 2m fluorochrome conjugate were used in exchange reactions to demonstrate that beta 2m associates with a teleostean cell surface protein. beta 2m exchange among brown bullhead, channel catfish, fathead minnow, and rainbow trout cells lines was detected by using either radioimmunoassay or flow cytometry. evidence that beta 2m binds specifically with the surface of teleostean cells and possibly associates with an expressed class i mhc h ... | 1994 | 7768315 |
| protection against enteric septicemia of catfish (ictalurus punctatus) by immunization with the r-mutant, escherichia coli (j5). | the protective effects of immunization with the rough (r) mutant, escherichia coli (j5), were evaluated in an experimental model of edwardsiella ictaluri-induced enteric septicemia of catfish (ictalurus punctatus). groups of 18 to 25 fish were administered killed e coli (j5) with or without an aluminum hydroxide (alum) adjuvant by intraperitoneal (i.p.) or im route. vaccinated fish were experimentally infected with virulent ed ictaluri. fish were monitored to determine survival after ed ictaluri ... | 1994 | 7802393 |
| isolation and characterization of the cdna encoding the channel catfish (ictalurus punctatus) form of cytochrome p450arom. | cytochrome p450arom (aromatase) is responsible for the conversion of androgens to estrogens. a cdna library was constructed from poly(a)-enriched mrna isolated from channel catfish (ictalurus punctatus) ovary and ligated into ecori-cut lambda arms. the amplified library was screened using a human aromatase dna probe. the longest clone isolated (2.1 kb) contained 20 bp of the 5'-untranslated region, a 1572-bp open reading frame, and a 509-bp 3'-untranslated region. northern blot analysis indicate ... | 1994 | 7958747 |
| functionally distinct high and low molecular weight species of channel catfish and mouse il-1. | culture supernatants from channel catfish monocytes exhibit il-1-like activity for mouse and catfish t cells. gel filtration analyses of these supernatants indicated that there were at least two forms of il-1-like activity, i.e. a high molecular weight form (70 kd) active on channel catfish, but not mouse, t cells and a low molecular weight form (approximately 15 kd) with activity for mouse, but not catfish, t cells. both sizes of catfish il-1 exhibited alpha and beta determinants as shown by we ... | 1994 | 8003627 |
| experimental feeding of fusarium moniliforme culture material containing fumonisin b1 to channel catfish, ictalurus punctatus. | 1994 | 8011771 | |
| channel catfish herpesvirus (ccv) encodes a functional thymidine kinase gene: elucidation of a point mutation that confers resistance to ara-t. | the channel catfish herpesvirus (ccv) thymidine kinase (tk) gene was mapped on the ccv genome by marker rescue analysis using a tk-deficient channel catfish ovary cell line (cco), a tk-negative ccv mutant, and a panel of cloned ccv genomic dna fragments. the tk-deficient cell line (ccobr) was isolated after repeated propagation of cco cells in increasing concentrations of 5-bromo-2'-deoxyuridine. infection of ccobr cells with ccv produced high levels of tk activity. the tk- virus (ccvar) was iso ... | 1994 | 8030231 |
| a beta-glucan inhibitable zymosan receptor on channel catfish neutrophils. | in mice and humans zymosan binds to the complement receptor three/mac-1 receptor; however, identification of this receptor in channel catfish (ictalurus punctatus) has not been accomplished. soluble fluorescein isothiocyanate (fitc) conjugated beta-glucan, a component of zymosan, was found to bind to channel catfish anterior kidney (ak) neutrophils but not to b-lymphocytes. serum activated zymosan (saz)-mediated chemiluminescence responses of channel catfish ak neutrophils could be inhibited by ... | 1994 | 8066990 |
| expressed sequence tags (est) identify genes preferentially expressed in catfish chemosensory tissues. | expressed sequence tags (ests) for the catfish (ictalurus punctatus) were identified and characterized by shotgun sequencing coupled to northern analysis. we have identified and characterized a number of cdna clones from a catfish olfactory mucosal library that show differential tissue expression including several that are enriched in chemosensory tissue. among the novel cdna clones studied were an olfactory specific beta-tubulin and a novel member of the s-100 family of calcium-binding proteins ... | 1994 | 8090068 |
| glutathione-dependent defense in channel catfish (ictalurus punctatus) and brown bullhead (ameriurus nebulosus). | glutathione-dependent defense against xenobiotic toxicity is a multifaceted phenomenon that has been well characterized in mammals. this study undertakes a comparison of two benthic fish species, the channel catfish and brown bullhead, in terms of characteristics of the glutathione system. the channel catfish, a species that has seldom been observed to express pollutant-mediated neoplasia in field studies, was observed to have significantly higher constitutive levels of hepatic total glutathione ... | 1994 | 7523070 |
| organization of inner ear endorgan projections in the goldfish, carassius auratus. | cytoarchitectural analysis of the octavolateralis area of the goldfish, carassius auratus, reveals that as in other teleosts, five first-order octaval nuclei are present: the anterior octaval, magnocellular, descending, tangential, and posterior octaval nuclei. the descending nucleus appears to be anatomically specialized relative to that of the halecomorph amia calva and many teleosts in that a large dorsomedial subpopulation of the nucleus lies medial to nucleus medialis, a first-order lateral ... | 1994 | 8038983 |
| diencephalic gustatory connections in the channel catfish. | vertebrate gustatory systems include a tertiary ascending pathway from a secondary gustatory nucleus in the hindbrain to several forebrain nuclei. this connection is prominent in catfish, corresponding to their highly developed sense of taste. iontophoretic injections of horseradish peroxidase were used to identify the specific target nuclei of the tertiary gustatory pathway in channel catfish and to characterize those nuclei by their respective connections. efferents from the secondary gustator ... | 1993 | 7506715 |
| the primary octaval nuclei and inner ear afferent projections in the otophysan ictalurus punctatus. | physical coupling between the swimbladder and the inner ear is believed to contribute to the enhanced hearing abilities of otophysans relative to those of teleosts lacking comparable otic specializations. we hypothesized that the auditory circuitry of otophysans might also exhibit derived features [mccormick and braford, 1988]. as a first test of this hypothesis, we examined the normal anatomy and inner ear inputs of the primary octaval nuclei in the catfish ictalurus punctatus. from nissl-stain ... | 1993 | 8324624 |
| opsonic effect of the alternative complement pathway on channel catfish peripheral blood phagocytes. | this study determined the effect of the alternative complement pathway (acp) on neutrophil and monocyte phagocytes from peripheral blood of channel catfish, ictalurus punctatus. fluorescent-labeled latex microspheres, edwardsiella ictaluri and escherichia coli were used to quantify phagocytic attachment and ingestion. activation of the acp enhanced the attachment of bacteria and microspheres to neutrophils and monocytes. activation of the acp by serum opsonization of escherichia coli increased i ... | 1993 | 8116220 |
| expression of a mouse-channel catfish chimeric igm molecule in a mouse myeloma cell. | fusion genes encoding a murine vh domain and the constant region domains of the mu chain from the channel catfish, ictalurus punctatus, were stably expressed in the lambda light chain producing mouse myeloma cell line j558l. although the pathways of pre-mrna processing for expression of membrane (micron and secreted (microsecond) forms of the mu chain differ between mammals and teleosts, mrnas encoding both catfish micron and microsecond were correctly expressed in the mouse myeloma cells. the m ... | 1993 | 8232326 |
| structure of the channel catfish (ictalurus punctatus) growth hormone gene and its evolutionary implications. | a dna fragment of 1.6 kilo base pairs (kb), encoding part of the channel catfish (ictalurus punctatus) growth hormone (gh) gene, was generated by the polymerase chain reaction (pcr) using 2 degenerate synthetic oligonucleotides (30 and 33 mer) derived from the n- and c-terminal amino acid sequences of the catfish gh polypeptide as amplification primers and with catfish genomic dna as a template. this dna fragment was used as a probe for the isolation of a catfish gh gene from a genomic library c ... | 1993 | 8293072 |
| rapid enzyme-linked immunosorbent tests for detecting antibodies to edwardsiella ictaluri in channel catfish, ictalurus punctatus, using exoantigen. | a rapid enzyme-linked immunosorbent assay (elisa) was developed to evaluate sera from channel catfish with and without edwardsiella ictaluri infection. anti-e. ictaluri antibodies were detected in the sera from 94 of 100 infected catfish. specific antibodies were not detected in the sera from 100 non-infected fish. the elisa was based on the falcon assay screening test (fast) and was performed in 30 min. an e. ictaluri exoantigen was used in the fast-elisa. the immunodominance of the exoantigen ... | 1993 | 8333144 |
| sequence of two eco ri fragments from salmonis herpesvirus 2 and comparison with ictalurid herpesvirus 1. | two eco ri fragments from salmonis herpesvirus 2 (oncorhynchus masou virus) have been sequenced. clone poe 150 coded for a protein homologous to orf 68 from ictalurid herpesvirus 1 (channel catfish virus). clone poe21 coded for a protein homologous with orf46 from the same virus. no homology was found with mammalian herpesviruses. | 1993 | 8379857 |
| channel catfish liver monooxygenases. immunological characterization of constitutive cytochromes p450 and the absence of active flavin-containing monooxygenases. | multiple drugs and pesticides are used in the aquaculture of channel catfish in the southeastern united states. however, little is known regarding the enzymatic metabolism of these chemicals in the fish. western blots, utilizing polyclonal antibodies raised against five purified rainbow trout liver cytochrome p450 enzymes, revealed at least two protein bands that were approximately 50 kda (catl-1) and 53 kda (catl-2). anti-trout lmc3 and lmc4 only hybridized with the 53 kda protein, whereas anti ... | 1993 | 8424815 |
| productive infection of continuous lines of channel catfish leukocytes by channel catfish virus. | channel catfish virus (ccv) undergoes extensive replication in fingerling catfish and establishes latent infection in survivors. although the site of viral latency in carriers is unknown, a variety of tissues, including leukocytes, have been implicated. to explore the interaction of ccv and leukocytes further, we examined in vitro infection of continuous lines of cloned catfish b cells and non-cloned lines containing predominantly macrophages and putative t-cells. our results indicate that all t ... | 1993 | 8460502 |
| the family of genes encoding odorant receptors in the channel catfish. | the anatomical and numerical simplicity of the fish olfactory system has led us to examine the family of olfactory receptors expressed in the catfish. we have identified a family of genes encoding seven transmembrane domain receptors that share considerable homology with the odorant receptors of the rat. the size of the catfish receptor repertoire appears to be far smaller than in mammals. analysis of the nucleotide sequences suggests that these receptor genes have undergone positive darwinian s ... | 1993 | 7916654 |
| phylogeny of immune recognition: fine specificity of fish immune repertoires to cytochrome c. | using the structurally defined protein antigen cytochrome c, studies were conducted in an attempt to delineate the fine specificities of channel catfish immune repertoires. we have previously reported that species variants of cytochrome c were cross-stimulatory to peripheral blood leukocytes (pbl) from catfish immunized with the pigeon variant. molecular database analyses revealed the existence of overlapping epitopes that appear to define the specificity of the immune response to a "family" of ... | 1993 | 7686867 |
| plasmid and serological differences between edwardsiella ictaluri strains. | several studies have shown that isolates of edwardsiella ictaluri obtained from infected channel catfish in the southeastern united states harbor two cryptic plasmids, designated pcl1 (5.7 kb) and pcl2 (4.9 kb). these isolates appear to be serologically homogeneous. to extend these studies, we focused our analyses on two isolates of nonictalurid origin. plasmid analyses of a danio isolate showed that it harbored plasmids which were similar if not identical to pcl1 and pcl2. this strain was also ... | 1993 | 7692817 |
| channel catfish virus: a new type of herpesvirus. | herpesviruses are large double-stranded dna viruses which infect vertebrates from fish to man. convincing genetic relationships between herpesviruses that infect higher vertebrates (birds and mammals) have been demonstrated previously by comparing proteins predicted from dna sequences and have been interpreted as a result of evolution from a common ancestor. in order to evaluate how herpesviruses of lower vertebrates fit into this scheme, the 134,226-bp genome of channel catfish virus was sequen ... | 1992 | 1727613 |
| chemical identification of catfish growth hormone and prolactin. | isolation and primary structure of growth hormone (gh) and prolactin (prl) from the pituitary gland of catfish (ictalurus punctatus) are described. alkaline extract of the pituitary glands was fractionated by gel filtration on sephadex g-75, ion-exchange chromatography on deae-cellulose, and reversed-phase high-performance liquid chromatography on octadecyl silica ods. catfish gh and prl were identified by western blotting with antisera against chum salmon gh and prl. the catfish gh consists of ... | 1992 | 1308206 |
| electroporation: a method for transferring genes into the gametes of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio). | recombinant plasmids containing the rous sarcoma virus long-terminal repeat (rsvltr) promoter linked to either rainbow trout (oncorhyncus mykiss) growth hormone 1 (rtgh1) or growth hormone 2 (rtgh2) cdna were linearized and introduced into the fertilized eggs of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio) by both electroporation and microinjection. the latter two species had these rainbow trout constructs (rsvltr-rtgh1cdna or rsvltr-rtg ... | 1992 | 1339228 |
| purification and partial characterization of immobilization antigens from ichthyophthirius multifiliis. | ichthyophthirius multifiliis, a parasitic ciliate of freshwater fishes, was found to have surface antigens (ag) which elicited immobilizing antibodies (ab) when injected into rabbits. an effort was made to purify and characterize these ag (referred to as immobilization ag) because of their potential role in protective immunity in fishes. mice immunized with theront cilia were used for production of immobilizing monoclonal antibodies (mab). hybridomas were screened by indirect immunofluorescent l ... | 1992 | 1383510 |
| molecular genetics of sex determination in channel catfish: studies on sry, zfy, bkm, and human telomeric repeats. | in amniotes, the banded krait minor (bkm) minisatellite (gata), the human telometric sequence (ttaggg)7, and the y-specific genes, zfy and sry, are associated with a particular sex. these sequences were studied in the channel catfish, ictalurus punctatus. however, none was sex-specific in catfish; homologs of each were present in males and females. our data suggest that components of mammalian sex-determining systems may be widespread and shared among the vertebrates in general. whether those co ... | 1992 | 1391325 |
| novel heme-binding component in the serum of the channel catfish (ictalurus punctatus). | the serum of the channel catfish (ictalurus punctatus) was examined for heme- and hemoglobin-binding proteins. electrophoretic mobility retardation assays failed to detect a hemoglobin-binding material similar to mammalian haptoglobin; however, a heme-binding component (not previously described) was identified in catfish serum. the heme-binding component was purified by gel filtration chromatography; electrophoretic analyses suggested it to be composed of two polypeptide subunits of molecular ma ... | 1992 | 1392473 |
| isolation of an acute-phase phosphorylcholine-reactive pentraxin from channel catfish (ictalurus punctatus). | 1. channel catfish (ictalurus punctatus) serum contains a protein that precipitates pneumococcal c-polysaccharide (cps) in a calcium-dependent fashion. 2. the serum titer of this protein follows an acute-phase pattern in catfish injected with turpentine. 3. a non-glycosylated, phosphorylcholine (pc)-reactive protein (prp) with molecular mass ca 100 kda, was isolated from channel catfish acute-phase sera by affinity chromatography on pc-sepharose 4b. 4. contaminating proteins with molecular masse ... | 1992 | 1499290 |
| preliminary biological characterization of a melanization stimulating factor (msf) from the dorsal skin of the channel catfish, ictalurus punctatus. | a two step fractionation of conditioned media made from the darkly pigmented dorsal skin of the channel catfish, ictalurus punctatus, has produced fractions that contain a melanization stimulating factor (msf). isolated neural tubes of xenopus laevis embryos exposed to conditioned media and to specific fractions exhibit greater melanization (increased numbers of melanized cells and elevated percentages of melanized cells), a greater number of dendrites per melanized cell, and a greater number of ... | 1992 | 1528091 |
| summary of bacterial isolates from farm-reared channel catfish (1979-1988). | 1992 | 1616987 | |
| ascending general visceral pathways within the brainstems of two teleost fishes: ictalurus punctatus and carassius auratus. | the primary general visceral nucleus in goldfish (carassius auratus) and catfish (ictalurus punctatus) is located at the ventroposterior boundary of the vagal gustatory lobe and receives coelomic visceral, but not gustatory inputs. the neuronal tracer horseradish peroxidase (hrp) was employed to visualize sources of input to and ascending projections from the primary general visceral nucleus in these species. in addition, immunocytochemical techniques were utilized to define the cytological divi ... | 1992 | 1629401 |
| sphaerospora infection of american catfishes (ictalurus punctatus and i. nebulosus) in europe. | the occurrence in europe of a sphaerospora species described in north america is reported. based upon its morphological characteristics, the parasite could be identified with the species s. hankai described from brown bullhead in canada. this parasite was found to infect channel catfish (ictalurus punctatus) cultured in farm ponds in italy and brown bullhead (ictalurus nebulosus) living in the supply channels of fish ponds in hungary. the spores and sporogonic developmental stages were situated ... | 1992 | 1298164 |
| nutrition of aquaculture species. | dietary requirements for amino acids and fatty acids have been reported for channel catfish (ictalurus punctatus), salmonids (oncorhynchus spp.), common carp (cyprinus carpio), tilapias (oreochromis spp.), and eel (anguilla japonicus). most of the vitamin and mineral requirements are available for channel catfish and salmonids, and some are available for common carp, tilapia, eel, and other finfish and crustaceans. from this available information, cost-effective feeds can be formulated for the m ... | 1991 | 1778835 |
| use of polymerase chain reaction to detect latent channel catfish virus. | polymerase chain reaction was used to detect an economically important herpesvirus, channel catfish virus (ccv). a segment of the viral dna was sequenced and oligonucleotide primers were produced from that sequence. after the primers were tested for the possibility of hybridization to catfish dna, they were used to prime the polymerase chain reaction, using pure ccv dna, ccv dna added to catfish dna, and dna from catfish infected and not infected with ccv. in all cases, the method proved to be s ... | 1991 | 1665023 |
| phylogeny of immune recognition: processing and presentation of structurally defined proteins in channel catfish immune responses. | this work was undertaken to investigate whether or not antigen processing and presentation are important in channel catfish in vitro secondary immune responses elicited with structurally defined proteins, namely, pigeon heart cytochrome c (pcytc), hen egg lysozyme, and horse myoglobin. the use of in vitro antigen-pulsed and fixed b cells or monocytes as antigen presenting cells (apc) resulted in autologous peripheral blood leukocytes (pbl) responding with vigorous proliferation and antibody prod ... | 1991 | 1668258 |
| transduction mechanisms for the taste of amino acids. | amino acids are important taste stimuli for a variety of animals. one animal model, the channel catfish, i. punctatus, possesses sensitive taste receptor systems for several amino acids. neurophysiological and biochemical receptor binding studies suggest the presence of at least three receptor pathways: one is a relatively nonspecific site(s) responsive to short-chain neutral amino acids such as l-alanine (l-ala); another is responsive to the basic amino acid l-arginine (l-arg); still another is ... | 1991 | 1679559 |
| effect of temperature on the immune system of channel catfish (ictalurus punctatus)--ii. adaptation of anterior kidney phagocytes to 10 degrees c. | 1. anterior kidney phagocytes from channel catfish (ictalurus punctatus) exposed to 10 and 24 degrees c from 1 day to 5 weeks were assayed for phagocytic ability and respiratory burst activity to aeromonas hydrophila and edwardsiella ictaluri. 2. the results of this study indicated that phagocytosis in channel catfish remained partially functional at low temperature without adaptation, although partial suppression was observed. 3. adaptation to low temperature did lead to an improvement in the r ... | 1991 | 1685385 |
| a high-affinity zinc-binding plasma protein in channel catfish (ictalurus punctatus). | 1. channel catfish (ictalurus punctatus) have a remarkably high concentration of zinc (zn) in their blood serum, about 20 micrograms/ml. however, compared to mammals, the concentrations of zn in their tissues are not remarkable. the serum zn is dialyzable against a solution containing 1 mm edta. 2. following separation of serum proteins by gel-filtration most of the zn was recovered in a fraction with the same peak volume of elution for the zn and protein concentrations and having a molecular we ... | 1991 | 1687545 |
| bacillus stearothermophilus disk assay for determining ampicillin residues in fish muscle. | the bacillus stearothermophilus disk assay for penicillin in milk (aoac official method) was adapted for the determination of ampicillin in fish muscle. the method was evaluated in 2 species of cultured fish: channel catfish and striped bass. recoveries of ampicillin ranged from 99 to 104% when muscle specimens from both species were spiked at concentrations of 0.025-1.00 micrograms/g. the lower limit of determination (lod) was 0.025 micrograms/g. the assay was applied to monitor the elimination ... | 1991 | 1757413 |
| s-layer positive motile aeromonads isolated from channel catfish. | motile aeromonads are ubiquitous aquatic bacteria that can cause motile aeromonad septicemia (mas), a disease which affects channel catfish and can produce significant economic loss. motile aeromonads isolated from commercially-raised channel catfish were screened for production of s-layer protein in order to evaluate its potential role in natural epizootics. the s-layer protein was produced by 14 of 24 (58%) isolates from epizootics evaluated in this study. concomitant infections with other int ... | 1991 | 1758021 |
| experience in shotgun sequencing a 134 kilobase pair dna molecule. | until now, large dna sequences have been obtained by cloning fragments of the target molecule into plasmid, cosmid or bacteriophage lambda vectors. the 134 kbp dna sequence of channel catfish virus was determined with relative ease by shotgun cloning of random fragments of genomic dna directly into a bacteriophage m13 vector, sequencing by dideoxynucleotide chain termination, and compilation of the data using staden's database handling programs. experience gained during this endeavour indicates ... | 1991 | 1768862 |
| biochemical and serological characterization of carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish. | a comparative analysis of the phenotypic and serological properties of carnobacterium strains associated with mortalities of cultured striped bass and channel catfish and the properties of isolates from wild brown bullhead catfish in the chesapeake bay area in maryland was conducted. all of the strains were gram-positive, facultatively anaerobic, nonmotile, non-spore-forming rods occurring singly or in short chains. they did not produce cytochrome oxidase or catalase, did not reduce nitrate, fai ... | 1991 | 1781676 |
| the genome of human herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. | more than 50 fragments resulting from complete digestion of the dna of human herpesvirus 6 (hhv-6, strain u1102) with bamhi, ecori, hindiii, kpni, nrui, sali or smai have been isolated as clones in m13, plasmid, cosmid and lambda vectors. using these clones, maps have been constructed for the fragments produced by nine restriction enzymes from unit-length virus genomes and from their concatemeric precursors. the unit-length genome is a linear, double-stranded molecule of 161.5 kbp composed of a ... | 1991 | 1846644 |
| spontaneous development of functionally active long-term monocytelike cell lines from channel catfish. | during the course of studies involving the in vitro manipulation of channel catfish peripheral blood leukocytes, spontaneous proliferation was observed with unexpectedly high frequency. propagation of these spontaneously proliferating cells has resulted in the development of long-term (greater than 11 mo.) cell lines which stain positively for nonspecific esterase and peroxidase, are phagocytic for latex beads, and morphologically resemble mammalian monocytes or macrophages. these long-term cell ... | 1991 | 1856153 |
| granulomatous inflammation and monstrous giant cells in response to intraperitoneal hormone implants in channel catfish (ictalurus punctatus). | plastic implants (2.7 mm maximum dimension) of an ethyl vinyl acetate copolymer (evac) matrix, containing inulin, bovine serum albumin (bsa) and luteinizing hormone releasing hormone (lhrh), were covered with impervious evac and then surgically placed into the peritoneal cavity of 1-year-old channel catfish, ictalurus punctatus. in fish kept in cold water (13 degrees c), 10 per cent of the implants per month were encapsulated by granulation tissue. in fish kept in warm water (27 degrees c), 20 p ... | 1991 | 1865025 |
| evolution of herpesvirus thymidine kinases from cellular deoxycytidine kinase. | the thymidine kinases encoded by herpesviruses of higher vertebrates form a distinct group and are unrelated to the thymidine kinases (tks) of other organisms. their evolutionary source has not been identified, but our analysis has revealed a clear relationship with a sequence of human deoxycytidine kinase (dck) published recently. we report the sequence of the putative tk of channel catfish virus, a herpesvirus of a lower vertebrate, and show that it is also related to dck. we propose, therefor ... | 1991 | 1919533 |
| measurement and prediction of digestible energy values in feedstuffs for the herbivorous fish tilapia (oreochromis niloticus linn.). | digestible energy (de) values were measured in a selection of feedstuffs for the tilapia (oreochromis niloticus linn.) and used to develop equations for predicting de values of a wider range of feedstuffs from chemical analyses. preliminary work examined the influences of substitution level in a reference diet and adaptation over time on de values for soya-bean meal. length of adaptation period significantly affected de values (p less than 0.01), but substitution level, over the range 200-600 g ... | 1991 | 1931904 |
| heavy chain variable region gene families evolved early in phylogeny. ig complexity in fish. | the v regions of channel catfish h chain cdna clones have been analyzed. based upon sequence relationships and hybridization analyses, five different groups of vh genes are identified whose definition is consistent with that of five different vh families. genomic southern blots indicate that as many as 100 different germ-line vh genes are likely represented by these families. the sequence diversity between identified members of these different families is similar in magnitude to the divergence r ... | 1991 | 1988492 |
| fish mortality in the mississippi catfish farming industry in 1988: causes and treatments. | the 1988 fish mortality summary for the catfish (ictalurus punctatus) industry in mississippi is presented. in 1988, 2,456 cases were submitted to mississippi cooperative extension service fish disease laboratories at belzoni and stoneville. bacterial infection caused by edwardsiella ictaluri was the leading cause of catfish mortality. descriptions and treatments are presented for bacterial, parasitic, viral and other diseases affecting mississippi farm-raised catfish in 1988. | 1991 | 2023314 |
| in vitro responses of channel catfish, ictalurus punctatus, neutrophils to edwardsiella ictaluri. | the in vitro responses of channel catfish peripheral blood neutrophils to edwardsiella ictaluri were examined through the use of phagocytic, bactericidal, and chemiluminescent assays. evidence from both light and electron microscopy indicate that catfish neutrophils appeared to phagocytose e. ictaluri. although extracellular killing of e. ictaluri was observed, bactericidal assays did not demonstrate intracellular killing of e. ictaluri by neutrophils. catfish neutrophils mount a chemiluminescen ... | 1991 | 2050246 |
| fish cell lines: establishment and characterization of three new cell lines from grass carp (ctenopharyngodon idella). | three new cell lines were established from tissues of the grass carp, ctenopharyngodon idella. derived from the fin, snout, and swim bladder of two apparently healthy diploid fry, these cell lines have been designated gcf, gcs-2, and gcsb, respectively. the cells grew at temperatures between 24 degrees and 36 degrees c with optimal growth at 32 degrees c and have been subcultured more than 50 times since their initiation in august 1986. two of the lines remained diploid or pseudodiploid after 38 ... | 1990 | 2108122 |
| effect of size and temperature on the quantity of immunoglobulin in channel catfish, ictalurus punctatus. | concentrations of serum immunoglobulin (ig) were determined for 30 channel catfish from pond water at 10 degrees c. these values were compared to measurements of 15 channel catfish from pond water at 30 degrees c. channel catfish from 10 degrees c pond water had no significant (p greater than 0.05) different ig concentrations (mean, 398 mg/dl) than catfish from 30 degrees c pond water (mean, 367 mg/dl). serum ig concentrations appear not to be different in cold (10 degrees c) vs warm (30 degrees ... | 1990 | 2110694 |
| effect of low-temperature culture and l3t4 antibody on the survival of pancreatic islets xenograft (fish to mouse). | catfish (ictalurus punctatus) islets were transplanted into the renal subcapsular space of diabetic c57bl/6j mice. the xenograft mean survival time (mst) of islets cultured for 6 days at 24 degrees c was 4.9 +/- 0.5 days, while xenotransplantation of 1-hour-cultured islets did not restore normoglycemia in the diabetic mice. a modest but significant prolongation of the mst was obtained by administration of anti-l3t4 monoclonal antibody to the recipients (13.4 +/- 2.1 days). anti-l3t4 in conjuncti ... | 1990 | 2116970 |
| membrane damage to escherichia coli and bactericidal kinetics by the alternative complement pathway of channel catfish. | 1. increased permeability of cytoplasmic membranes in escherichia coli was a consequence of alternative complement pathway (acp) activity of serum of channel catfish, ictalurus punctatus. evidence was provided by beta-galactosidase activity extracellularly when e. coli was incubated with catfish serum. 2. lesions were detected on outer membranes of e. coli following exposure to catfish serum. 3. catfish acp induced a temporal sequence of pre-killing and killing phases. 4. loss of cell viability, ... | 1990 | 2286064 |
| a protein kinase-related gene within the channel catfish herpesvirus genome. | 1990 | 2349101 | |
| differences in the phagocytosis of four bacteria by channel catfish neutrophils. | the purpose of this study was to compare the phagocytosis of aeromonas hydrophila, edwardsiella ictaluri, edwardsiella tarda and micrococcus luteus by channel catfish neutrophils. various aspects of opsonization effect and bactericidal ability of channel catfish neutrophils were investigated. percent phagocytosis ranged from a low of 1% to a high of 91%. the highest percent phagocytosis and phagocytic indices routinely occurred with normal serum and were highest for m. luteus (91.78%, 55.25) and ... | 1990 | 2369968 |
| outer membrane protein profiles of edwardsiella ictaluri from fish. | outer membrane proteins (omp) prepared with sodium n-lauroyl sarcocinate (sls) from 33 edwardsiella ictaluri isolates from fish were examined by electrophoresis. twenty-eight isolates from channel catfish (ictalurus punctatus) had similar omp profiles. ten bands (71 kilodaltons [kd] to 19.5 kd) were identified in all isolates from channel catfish. one major 35-kd protein comprised most of the protein content of the outer membrane of isolates from channel catfish. differences existed among isolat ... | 1990 | 1689127 |