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emerging bacterial zoonotic and vector-borne diseases. ecological and epidemiological factors.among the etiologic agents of emerging infectious diseases are several bacterial organisms that naturally reside in animal and arthropod hosts. the most compelling emerging bacterial zoonotic and vector-borne diseases in the united states are lyme disease; a southern erythema migrans-like illness; human monocytic ehrlichiosis; human granulocytic ehrlichiosis; a novel cat flea-associated typhus group rickettsiosis; bartonelloses of immunocompetent and immunocompromised persons, particularly with ...19968627968
[the state of in vitro breeding of lice and fleas]laboratory breeding of lice, fleas and other bloodsucking arthropods is necessary for developing and testing the efficacy of new drugs and alternative arthropod control methods. they are necessary to investigate their role as vectors of pathogens and to develop methods for diagnosis and therapy of arthropod caused or transmitted diseases. to date, it is not possible to replace rabbits as natural blood sources for breeding lice colonies (pediculus spp.). but on the base of the knowledge about lic ...199611178453
effect of combing time on cat flea (ctenocephalides felis) recovery from dogs.combing the haircoat to count fleas has been used to determine the efficacy of insecticides against fleas on dogs, but no standardization of method has been reported. in this study, the effect of combing time on flea recovery from dogs was examined. six beagle dogs were infested with 100 unfed, adult ctenocephalides felis (bouché) on each of three consecutive days. a crossover design, balanced for carryover effects, was used to evaluate flea removal rates from each dog by comb-counting for three ...19958644450
disinfestation of experimentally infested cat fleas, ctenocephalides felis, on cats and dogs by oral lufenuron.in vivo efficacy of orally administered lufenuron, an insect growth regulator, in disinfesting cat fleas was evaluated, using flea-free cats and dogs which were purchased and infested every 10 days with cat fleas from a colony kept in our laboratory. lufenuron was orally administered as a single dose of 15, 30, or 60 mg/kg to cats, and 5, 10 or 20 mg/kg to dogs. in cats, adult flea emergence was intensively prevented for 30 days by dosing of 15 mg/kg of lufenuron and 40 days by 30 or 60 mg/kg. t ...19958519893
the cat, the flea, and pesticides.recent advances in our knowledge of cat flea biology and the discovery of new nontoxic control methods have greatly simplified and improved flea control. instead of relying on adulticides for the bulk of flea control, the veterinarian now focuses on the immature stages, particularly the larvae and the eggs. by doing this, environmentally "friendly" flea control methods are employed that are much safer for the veterinarian, the pet owner, and the cat.19958525567
airborne insecticide residues after broadcast application for cat flea (siphonaptera: pulicidae) control.airborne residues of chlorpyrifos, propetamphos, and permethrin were measured up to 50 h after broadcast treatment for cat flea, ctenocephalides felis (bouché), control in residences. type of insecticide, time after treatment, ventilation regime, and height above floor significantly affected airborne residues. before spraying chlorpyrifos, residues were low or undetected. in nonventilated residences, chlorpyrifos residues peaked 0-6 h after treatment at 38 ng/liter, then slowly declined through ...19958537544
fleas, lice and mites on scrub hares (lepus saxatilis) in northern and eastern transvaal and in kwazulu-natal, south africa.fleas, lice and mites were collected from 24 and 120 scrub hares at pafuri and skukuza, northern and eastern transvaal, respectively, in the kruger national park, and from 34 scrub hares in the hluhluwe region, north-eastern kwazulu-natal. ctenocephalides felis damarensis, the only flea recovered, reached peak burdens on the hares at each locality during late winter or spring. juvenile hares harboured significantly fewer fleas than did adult animals. the lice haemodipsus lyriocephalus and haemod ...19958600437
chlorpyrifos formulation effect on airborne residues following broadcast application for cat flea (siphonaptera: pulicidae) control.airborne residues of 3 chlorpyrifos formulations were measured up to 50 h after broadcast treatment for flea control in residences. insecticide formulation, time after treatment, ventilation regime, and height above floor affected airborne residues. before spraying, chlorpyrifos residues were low or undetected. in nonventilated residences, chlorpyrifos residues peaked in 0-6 h after treatment at 38 ng/liter for emulsifiable, remained low at < 12 ng/liter for the microencapsulated, and sharply pe ...19957544814
isolation, cultivation, and partial characterization of the elb agent associated with cat fleas.elb rickettsiae from cat flea homogenates were recovered in tissue culture cells following sequential passage through laboratory rats and the yolk sacs of embryonated chicken eggs. seven days after inoculation of elb from the infected yolk sacs, vero cells and l929 cells were observed to contain intracellular bacteria as demonstrated by diff quik and indirect immunofluorescence assay staining. the rickettsial and elb identity of the cultured agent was confirmed by pcr detection of the 16s rrna a ...19957591142
species of flea found on cats and dogs in south west england: further evidence of their polyxenous state and implications for flea control.fleas were collected from 60 dogs and 32 cats living in south west england. ctenocephalides felis felis and ctenocephalides canis were found on both dogs and cats, with a marked preponderance of c felis felis on both species. more female fleas than males were found. there was no apparent tendency for c canis to be found more often in rural areas than in suburban areas. the survey confirmed the polyxenous nature of both species of flea. the significance of this state is discussed in relation to f ...19957610540
larvicidal effects of boric acid and disodium octaborate tetrahydrate to cat fleas (siphonaptera: pulicidae).borate products varied in efficacy against larval cat fleas, ctenocephalides felis felis bouché, with some effective at rates of 23 micrograms/cm2 when larvae were exposed to the compounds in sand. powdered boric acid, granular boric acid, and disodium octaborate tetrahydrate (polybor) at rates of 200 micrograms/cm2 produced < or = 90% mortality of larvae exposed in carpet for 96 h. lc50 values of larvae exposed in treated carpets for 96 h were 23 micrograms/cm2 for powdered boric acid, 40 micro ...19957650701
survival and reproduction of the cat flea (siphonaptera: pulicidae) fed human blood on an artificial membrane system.adult cat fleas, ctenocephalides felis (bouché), survive and reproduce when fed human blood through an artificial membrane system. when a dog hair substrate was included in cages with the fleas, mean adult mortality was 2.4 after 12 d of bloodfeeding. egg production began after 3 d and was continuous for 12 d, ranging from 3 to 4 eggs per female per day. in cages without hair, mean adult mortality was 61.2% after 12 d of bloodfeeding. egg production began after 2 d, reached a maximum of two eggs ...19957650707
residual effectiveness of insect growth regulators applied to carpet for control of cat flea (siphonaptera: pulicidae) larvae.three insect growth regulators, fenoxycarb, methoprene, and pyriproxyfen, formulated as total release aerosols, were tested for their residual effectiveness on carpet in bioassays with cat flea, ctenocephalides felis (bouché), larvae. all treatments except methoprene produced significant mortality for the 7-mo duration of the test. in general, fenoxycarb and the higher rates of pyriproxyfen reduced adult flea emergence by > 80%.19957657899
flea species from dogs and cats in northern greece: environmental and clinical implications.fleas were identified after being collected from 129 dogs and 38 cats of random breed, sex and age. all these animals, infested with fleas and admitted to the clinic of medicine of the veterinary faculty in thessaloniki for routine procedures, were from different habitats and originated from various parts of northern greece. ctenocephalides canis was the most common species found on the dogs (71.3%). conversely, its prevalence in the 14 cats was substantially lower (5.3%). ctenocephalides felis ...19957676591
a further comparison of the thumb-counting and comb-counting techniques used to determine ctenocephalides felis infestation levels on dogs.a comparison was made to determine whether thumb-counting or comb-counting was more accurate for determining flea infestation levels on dogs when performed for equal periods of time. to accomplish this, ten beagle dogs were each infested with 100 adult fleas, ctenocephalides felis. after the fleas were allowed to disperse for 1 h the dogs were examined using the thumb-counting method. the time required to cover each dog and the number of fleas counted were recorded. thumb-counting times ranged f ...19957754612
efficacy of lufenuron against developmental stages of fleas (ctenocephalides felis felis) in dogs housed in simulated home environments.twenty-four, adult, female beagles were arranged by body weight from greatest to least and allocated to 2 groups of 12 dogs, using random numbers. dogs were housed collectively in 2 adjacent metal buildings, each divided into 4 rooms measuring 2.1 x 3.7 m. each room was paneled and carpeted and had an access door to the outside with a connecting run that measured 2.1 x 9.1 m. each run had a surface consisting of 5 cm of pea gravel overlaying 5 cm of sand, and was partially covered by an awning t ...19957785823
horizontal transmission, vertical inactivation, and stochastic loss of mariner-like transposable elements.horizontal transmission has been well documented as a major mechanism for the dissemination of mariner-like elements (mles) among species. less well understood are mechanisms that limit vertical transmission of mles resulting in the "spotty" or discontinuous distribution observed in closely related species. in this article we present evidence that the genome of the common ancestor of the melanogaster species subgroup of drosophila contained an mle related to the mellifera (honey bee) subfamily. ...19957877497
adult cat flea (siphonaptera: pulicidae) excretion of host blood proteins in relation to larval nutrition.protein content and ion composition of host blood and feces from blood-fed adult cat fleas, ctenocephalides felis (bouché), were examined. total fecal protein differed slightly from host blood indicating that there was little digestion of host blood by adult fleas. sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of host blood and flea feces also revealed little digestion, as evidenced by the quantitatively and qualitatively similar protein banding patterns. blood and fecal ion com ...19947910638
techniques for estimating on-animal populations of ctenocephalides felis (siphonaptera: pulicidae).effectiveness of two sampling techniques to estimate cat flea populations were compared on beagles (short-haired dogs). overall, 23.5 and 81.6% of known flea populations on the beagles were detected using area and comb counting techniques, respectively. using comb counts, we were able to differentiate among beagles that were infested experimentally to produce densities of 21.6 (low), 42.2 (medium), and 82.5 (high) fleas per dog. using the area counting technique, we were able to differentiate be ...19947932613
evaluation of a single oral dose of lufenuron to control flea infestations in dogs.a single dose of lufenuron was administered to dogs to test its efficacy in controlling cat flea (ctenocephalides felis) infestations for at least 30 days. efficacy measurements revealed marked differences in the reproduction capability of fleas collected from dogs in the treatment vs the control group. essentially, all of the eggs collected from dogs treated with lufenuron were unable to develop into normal adult fleas. conversely, in the control group, 68.6% of the flea eggs developed into nor ...19947944022
murine typhus: updated roles of multiple urban components and a second typhuslike rickettsia.studies using serologic and polymerase chain reaction-(pcr) facilitated analysis of field samples from southern texas indicate the presence of rickettsia typhi and elb agent infected cat fleas, ctenocephalides felis (bouché), and the first observation of elb infected vertebrates (opossums). the elb agent is a recently described typhus-like rickettsia that is not distinguished from r. typhi or r. prowazekii by currently available serologic reagents. restriction digests of pcr products from 399 fl ...19947966170
flea allergy dermatitis in dogs diagnosed by intradermal skin tests.thirty-three dogs were categorised according to their clinical signs of flea allergy dermatitis and reactivity to feeding fleas (ctenocephalides felis felis). a soluble extract of whole fleas (fs), fractions of this extract separated by chromatography, and a commercially available flea antigen extract were used in intradermal skin tests (idst) to establish the presence or absence of type i and type iv hypersensitivity. the reactions were measured and the results were analysed using three grading ...19947973089
igg and ige antibodies against antigens of the cat flea, ctenocephalides felis felis, in sera of allergic and non-allergic dogs.antigens from a soluble extract of cat fleas (fs) were separated using sds-page, and transferred to nitrocellulose paper. sera from dogs shown by use of a provocative flea feeding test to be either allergic (23 dogs) or non-allergic (20 dogs) to flea bites, were used in western blots to identify flea antigens that react with canine igg or ige. the sera were also tested in elisa against fs to quantify levels of igg and ige antibodies. antibodies present in the sera of both flea allergic and non-a ...19948026904
the cat flea: biology, ecology and control.control failures and recurrences of infestation of dogs, cats and their home environment with the cat flea, ctenocephalides felis felis are common. attempts to control these infestations are often impaired by an inadequate understanding of the interaction of the cat flea with its hosts and environment. this review presents information on the medical and veterinary importance of the cat flea and discusses recent information on the environmental and host factors that affect its development and sur ...19948030176
interhost movement of adult cat fleas (siphonaptera: pulicidae).adult cat fleas, ctenocephalides felis (bouché), marked with orange-red or green fluorescent dye were released on one cat to study movement to another cat over a 7-d period. when the cats were separated, 3-8% of the marked fleas moved to the other cat. when the cats were allowed to remain together, 2-15% of the fleas moved. the percentage of fleas unaccounted for ranged from 8-47%. when both cats lived together, 8-15% movement occurred no matter which cat was infested. the percentage of female a ...19948057325
vaccination against the cat flea ctenocephalides felis felis.non-chemical control of haematophagous parasites is a desirable goal. we report here on the use of concealed antigens from the major digestive organ of the cat-flea as vaccine components. rabbits were immunized with various antigens from cat flea midguts, and immunoglobulin from these rabbits was fed to cat fleas in an artificial feeding system. antibody produced against soluble antigens from the midguts of fed fleas was able to kill fleas in this system. dogs were then immunized with various an ...19948058357
molecular identification of rickettsia-like microorganisms associated with colonized cat fleas (ctenocephalides felis).cat fleas (ctenocephalides felis) from eight commercial flea colonies from various regions of the usa were examined by selective pcr amplification, and subsequent restriction digest analysis and southern hybridization of pcr products, for the presence of a rickettsia-like organism (elb agent). these flea colonies were either started with fleas from one supplier (el labs), in which elb agent was first identified, or were started with fleas from stray cats and dogs and later came into contact with ...19948069413
comparison of thumb-counting and comb-counting methods to determine ctenocephalides felis infestation levels on dogs.comb-counting and thumb-counting were compared in a cross-over study to determine which was more accurate for quantifying flea infestation levels on dogs. twenty beagle dogs were used in the study and infested with either 50 or 100 adult fleas (ctenocephalides felis). two groups of five dogs each were infested with either 50 or 100 fleas per dog, and then comb-counted with a fine-toothed flea comb for 8 min periods. an additional two groups of five dogs each were also given 50 or 100 fleas, and ...19948091613
ultrastructure of the eggs of seven flea species (siphonaptera).eggs of seven flea species representing five families, ctenocephalides felis (bouché) (pulicidae), orchopeas leucopus (baker) and aetheca wagneri (baker) (ceratophyllidae), conorhinopsylla stanfordi stewart and epitedia faceta (rothschild) (hystrichopsyllidae), sternopsylla distincta texana (c. fox) (ischnopsyllidae), and craneopsylla minerva (rothschild) (stephanocircidae), were examined with a scanning electron microscope and are described from the resulting micrographs. in the pulicidae and c ...19947815392
ectoparasites of opossums and raccoons in southeastern georgia.twelve species of ectoparasites (four fleas, three ticks, three chiggers, one macronyssid mite, and one atopomelid mite) were recovered from 42 opossums, didelphis virginiana kerr, live-trapped from september 1992 through april 1994 in southeastern georgia. the fleas, ctenocephalides felis (bouché) (prevalence = 19%) and polygenis gwyni (c. fox) (36%); the ticks, dermacentor variabilis (say) (40%) and ixodes scapularis say (19%); and the macronyssid mite, ornithonyssus wernecki (fonseca) (21%), ...19947815408
oral toxicity of boric acid and other boron compounds to immature cat fleas (siphonaptera: pulicidae).oral toxicity was characterized in first-instar cat fleas, ctenocephalides felis felis (bouché), feeding on dried blood treated with boric acid. lc50 values ranged from 2.11% after 24 h to 0.21% after 7 d. in carpet tests with five different boron compounds and a number of different formulations, significant mortality for first instars was observed in all cases. in similar tests with prepupae and cocoons, there was no significant effect on mortality. the importance of these results is discussed ...19947836612
efficacy dosage titration of lufenuron against developmental stages of fleas (ctenocephalides felis felis) in cats.thirty-two mixed-breed male and female cats were blocked by sex, arranged by body weight from greatest to least, and allocated to 4 groups of 8 (4 male, 4 female) cats, using random numbers. cats in each of 3 groups were treated orally with a 7% suspension formulation of lufenuron at dosage of 15, 30, or 45 mg/kg of body weight. cats in the fourth group were treated orally with an excipient suspension without lufenuron. cats were infested with newly emerged, unfed cat fleas (ctenocephalides feli ...19948141503
cat flea (siphonaptera: pulicidae) cocoon formation and development of naked flea pupae.the rate of cat flea, ctenocephalides felis (bouché), pupal development and the stimuli responsible for successful cocoon formation were investigated by rearing flea pupae without an enclosing cocoon. female larvae pupated an average 12.1 h earlier than males, and female pupae developed to the adult stage an average 1.6 d earlier than males. when larvae were not allowed to orient against a perpendicular structure, only 2.2% of these larvae were able to spin an enclosing cocoon. although these na ...19948189417
efficacy of permethrin in controlling the australian paralysis tick ixodes holocyclus and the cat flea ctenocephalides felis on dogs. 19948198516
involvement of dogs in plague epidemiology in tanzania. serological observations in domestic animals in lushoto district.venous blood was collected aseptically from clinically healthy domestic dogs, goats, sheep, cats and fowl in various plague-infected villages of lushoto district, tanzania, at the time when the disease was actively prevalent in the area. flea ectoparasites were collected from the animals, processed, identified and counted. serum samples were tested for specific plague antibodies, using the passive haemagglutination technique and checked by passive haemagglutination inhibition tests. altogether 3 ...19938248751
development of a trap for collecting newly emerged ctenocephalides felis (siphonaptera: pulicidae) in homes.the visible light spectra most attractive to cat fleas, ctenocephalides felis (bouché), were determined. a green-yellow filter with a transmittance spectrum centered at 515-nm and a 82.5-nm half-height width attracted significantly more fleas than any other filter or white light. this information and observations on this flea's behavior were used to design a trap with a green-yellow filter and a light source with a 10-min:5-s (on and off) cycle. this trap collected > 86% of the live fleas releas ...19938254638
severe flea infestation in dairy calves.an investigation was conducted of a severe flea infestation in holstein dairy calves. inspection of the dairy revealed hundreds of cat fleas, ctenocephalides felis, on 23 calves, and thousands of fleas in the barn where the calves were housed. during the investigation, > 92,000 fleas were collected from the barn. the barn also was inhabited by 30 to 35 flea-infested domesticated and feral cats. several calves appeared emaciated, and analysis of blood samples from 10 calves revealed that 8 were a ...19938276708
an attempt to protect cats against infestation with ctenocephalides felis felis using gut membrane antigens as a vaccine.cats (n = 5) were vaccinated with membrane antigens extracted from the gut of unfed fleas (ctenocephalides felis felis) together with quil a and ribi as adjuvants. five unvaccinated cats were retained as controls. all the cats were infested on 6 separate occasions with fleas (46-250 per challenge). protection was assessed from the number of fleas retrieved and the fecundity of the female fleas, measured as the number of developed oocytes contained in the reproductive tract. cats injected with gu ...19938300299
[domestic cats as a source of human flea infestations in towns].during the summer and autumn periods of 1990-1992 a periodic massive incidence, infestation as well as parasitization of humans with flea ctenocephalides felis were recorded in the basements of flat blocks in the central area of kosice. the urban population of domestic cats served as a source of the infestation. the dark field examination of fleas for the presence of borrelias revealed negative results.19938306402
studies on the ectoparasites (fleas and lice) on rodents in riyadh and its surroundings, saudi arabia.a survey was conducted on rodents from the riyadh city and surroundings. two species of fleas, xenopsylla cheopis and ctenocephalides felis felis together with polyplax spinulosa were collected. the highest infestation rate by both fleas occurred on rattus rattus followed by rattus norvegicus and mus musculus. females of r. rattus and r. norvegicus were more infested by fleas than males. on the other hand, meriones spp., gerbillus spp., jaculus jaculus and acomys dimidiatus were infested with x. ...19938308348
fleas and lice on scrub hares (lepus saxatilis) in south africa.a total of 380 scrub hares (lepus saxatilis) from 5 localities, ranging from the north-eastern transvaal to the eastern and the south-western cape province of south africa, were examined for fleas and lice. ctenocephalides felis damarensis was almost exclusively responsible for the flea infestations on hares at 4 of the 5 sites. at 4 localities the prevalence and abundance of this flea reached peaks between august and october and declined thereafter to their lowest levels between february and ap ...19938332329
allergenicity of the cat flea. 19938334533
allergenicity of the cat flea (ctenocephalides felis felis)adult fleas, spent and unspent culture media were extracted and the radio-allergosorbent test (rast) performed with sera of 48 cat flea skin test-positive individuals from the tampa bay area of florida. sixteen sera (33.6%) had a positive rast to the cat flea extract prepared in our laboratory [1.7-11.4% of the total counts (tc) added]. six of the 16 sera (12.5%) also contained specific ige to allergens in the spent medium (0.8-3.3% tc). the allergen composition and strength were studied by rast ...19938334536
isolation and in vitro translation of messenger rna encoding allergens of the cat flea, ctenocephalides felis.polyadenylated [poly(a)+] mrna was isolated from the cat flea, ctenocephalides felis by oligothymidylic acid-cellulose spin column chromatography and translated in vitro using a cell-free rabbit reticulocyte system. the relative incorporation of 35s-methionine into trichloroacetic acid-precipitable translation products obtained using poly (a+) mrna was 48.5-fold over control translations performed without added mrna. sds-page analysis of the translation products in combination with autoradiograp ...19938342258
cloning and sequence analysis of the alpha subunit of the cat flea sodium pump.we have cloned a cdna that encodes the sodium pump alpha-subunit of the cat flea ctenocephalides felis. the deduced amino acid sequence of 1037 residues is 90% homologous with the drosophila alpha-subunit sequence and shows 75% similarity to the sheep kidney alpha-subunit. one characteristic unique to the insect polypeptides is an extended sequence at the n-terminus. northern blot analysis of c. felis rna detects two alpha-subunit transcripts with lengths of 3.8 and 4.4 kb. sodium pump alpha-sub ...19938397036
reproductive success of cat fleas, ctenocephalides felis, on calves as unusual hosts.cat fleas, ctenocephalides felis, were released onto calves as unusual hosts, and sampled at intervals for histological examination. egg output from fleas on age-matched male and female calves was monitored. using indicators of reproductive maturation and regression together with egg output data, the reproductive success and fertility of cat fleas on male and female calves were estimated. comparisons were made with fleas taken from cats. the mean egg output of fleas on the bull calf was highly s ...19938435496
characterization of allergens of the cat flea, ctenocephalides felis: detection and frequency of ige antibodies in canine sera.flea allergens, fractionated by polyacrylamide gel electrophoresis and transferred to nitrocellulose, were identified using 20 flea-allergic dog sera in an enhanced chemiluminescent assay for canine ige antibodies. at least 15 different flea components in the molecular weight range of 14-150 k bound ige and every serum demonstrated a different pattern of binding. three of the components with apparent molecular weights of 25, 40 and 58 k were each bound by at least 40% of the sera. no reactivity ...19938446466
a suburban focus of endemic typhus in los angeles county: association with seropositive domestic cats and opossums.thirty-three cases of locally acquired murine typhus were reported in los angeles county residents from may 1984 through february 1988. only eight cases were reported over the previous 20-year period. thirty (91%) cases resided within a suburban area encompassing approximately 50 km2 in northcentral los angeles or had contact with an animal from this area. serologic testing (complement fixation and indirect fluorescent antibody) of selected animals in close association with human cases revealed ...19938447530
mode of action of pyriproxyfen and methoprene on eggs of ctenocephalides felis (siphonaptera: pulicidae).adult cat fleas were exposed to residues of pyriproxyfen and methoprene in glass vials, then fed on a cat 24 h later to investigate the mode of action of juvenoid growth regulators on embryonic development in flea eggs. eggs laid by pyriproxyfen-treated fleas within 70 h after exposure to this juvenoid were often devoid of yolk and frequently collapsed after oviposition. minimal amounts of yolk were deposited in eggs laid after 70 h, and no blastoderm was formed. these results are significant be ...19938459420
efficacy of diflubenzuron in simulated household and yard conditions against the cat flea ctenocephalides felis (bouché) (siphonoptera: pulicidae).diflubenzuron-treated carpet inhibited egg-to-adult development of the cat flea for up to 12 mo even with weekly vacuuming of the carpet. the efficacy of diflubenzuron was similar in treated soils over a 6-mo test period except in the case of treated soil that remained outside. reduced efficacy is attributed to degradation of diflubenzuron by microorganisms and water.19938510122
patterns of erythrocyte digestion by bloodsucking insects: constraints on vector competence.two general patterns of erythrocyte digestion were observed in representative species from four insect orders. ingested erythrocytes were hemolyzed rapidly, and blood meals remained liquefied within body lice, pediculus humanus l. and the fleas ctenocephalides felis (bouché) and xenopyslla cheopis (rothschild). peritrophic membrane was absent. in contrast, there was a lag time of 6-18 h before substantial degradation of erythrocytes within the blood meals of bed bugs, cimex lectularius l.; the s ...19938094460
[notes on fleas (siphonaptera) in plague foci on the tay nguyen plateau (vietnam)].the fleas of wild and commensal small mammals, domestic animals (dogs, cats) and free-living flea forms in houses have been collected in plague nidi of tay nguyen plate, dak-lak province, vietnam. pulex irritans, ctenocephalides felis felis, ct. felis orientis were found in the houses on dogs, cats and on the ground floor. commensal rats in populated areas were infested by xenopsylla cheopis and rarely by lentistivalius klossi. the agricultural zone was inhabited by both home and wild animals su ...19921299806
distribution of split 5.8s ribosomal rna in diptera.although the 5.8s ribosomal rna (rrna) of most eucaryotes consists of 155-170 nucleotides, in two dipterous species the 5.8s rrna consists of two pieces, 123 and 30 nucleotides in length. the distribution of split 5.8s rrna was studied in other diptera and the most closely related order siphonaptera to learn the origin of split 5.8s rrna. four species of mosquitoes, culex tritaeniorhynchus, culex pipiens molestus, aedes albopictus, anopheles sp. (culicidae) had a single 5.8s rrna consisting of a ...19921343775
arthropoda as zoonoses and their implications.some aspects of changing patterns of arthropodal infections and arthropod-borne diseases in mediterranean areas are briefly discussed. selected examples are given, with particular emphasis on the phenomenon of the synanthropic flea ctenocephalides felis felis and on health problems caused by human infections with argas reflexus, the common tick of urban pigeons in europe. finally, the risk of the emergence of lyme borreliosis (borrelia burgdorferi) is considered in relation to the increasing spr ...19921441188
diel patterns of cat flea (siphonaptera: pulicidae) egg and fecal deposition.the quantity of eggs and feces deposited by replicate populations of cat fleas, ctenocephalides felis (bouché), from domestic house cats. felis catus, were recorded at 1-h intervals over a 24-h period, in a photoperiod of 12:12 (l:d) (photophase, 0600-1800 hours [est]). egg production averaged 23.96 +/- 0.83 eggs per female per day and was greatest from 2400 to 0300 hours and lowest from 0700 to 0900 hours and from 1700 to 1900 hours. daily fecal deposition was 0.77 +/- 0.03 mg feces per flea an ...19921495030
spectrophotometric method of quantifying adult cat flea (siphonaptera: pulicidae) feces.cat flea, ctenocephalides felis felis (bouché), feces are an essential part of larval nutrition under natural conditions. the mass values of adult flea feces can be measured by dissolving samples of flea feces in drabkin's reagent, filtering, centrifuging, and measuring absorbance spectrophotometrically at 540 nm. either flea feces or air-dried host blood can be used to generate the standard curve used to convert absorbance (optical density) values into mass values. debris collected from flea-in ...19921495033
influence of photoperiod on egg production of cat fleas (siphonaptera: pulicidae) infesting cats.photoperiod affected the production of cat flea eggs by cats infested with cat flea, ctenocephalides felis (bouché). cat flea eggs have a smooth chorion and do not adhere to the host. about 60% of fluorescing eggs placed in the pelage were recovered in collecting trays underneath the host in 2 h. an average of 87.7 eggs per cat was laid during the last 8 h of the scotophase compared with 49.9 and 59.1 eggs during the other 8-h periods. significant variation in the number of eggs collected daily ...19921495037
differential activity of cythioate against female and male ctenocephalides felis on cats.a study was conducted to determine whether cythioate, a systemically active insecticide, has different activity against male and female ctenocephalides felis, the cat flea. eighteen cats were allotted equally to 1 control and 2 treatment groups and infested on day 0 with fixed ratios of male and female cat fleas. cats in the untreated control group and treatment group 2 were infested with 50 fleas with a female-to-male ratio of approximately 2:1. cats in treatment group 1 were infested with 50 f ...19921524312
absence of ovicidal effects of fenoxycarb in the tick ixodes dammini as observed by light, scanning, and transmission electron microscopy.freshly oviposited eggs from fed females of the northern deer tick ixodes dammini were divided into two groups--untreated controls and fenoxycarb-exposed ova. the level of fenoxycarb exposure in the experimental group was equal to a concentration that proved 100% lethal in the cat flea, ctenocephalides felis (marchiondo et al. 1990). both groups were allowed to develop with samples taken at 48 and 144 h for examination by light microscopy, scanning electron microscopy, and transmission electron ...19921552518
effect of methoprene and diflubenzuron on larval development of the cat flea (siphonaptera: pulicidae).cat flea larvae, ctenocephalides felis bouche, exposed to glass surfaces treated with methoprene concentrations from 0.127 to 1,270 ng/cm2 did not emerge as adults. most larvae died in the third instar, but those exposed to the 0.127 ng/cm2 concentration formed larval-pupal intermediates. larvae exposed to glass surface treated with diflubenzuron concentrations from 12.7 to 1,270 ng/cm2 died during the process of molting in all three instars. exposure of larvae to 12.7 and 127 ng/cm2 diflubenzur ...19921607471
typhus and typhuslike rickettsiae associated with opossums and their fleas in los angeles county, california.the recent discovery of cat fleas (ctenocephalides felis) infected with a typhuslike rickettsia (designated the elb agent) raises the question of whether similar rickettsial infections exist in wild cat flea populations. we verified the presence of the elb agent and rickettsia typhi in urban and suburban areas of los angeles, calif. opossums trapped in close proximity to the residences of human murine typhus cases in los angeles county and other areas within the city of los angeles were tested f ...19921629332
genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas.the identification of apparently fastidious microorganisms is often problematic. dna from a rickettsia-like agent (called the elb agent) present in cat fleas could be amplified by pcr with conserved primers derived from rickettsial 17-kda common protein antigen and citrate synthase genes but not spotted fever group 190-kda antigen gene. alu i sites in both the 17-kda and citrate synthase pcr products obtained with the rickettsia-like agent and rickettsia typhi were different even though both age ...19921729713
indicators of sexual maturation and regression in the female cat flea, ctenocephalides felis.differences in the salivary glands, mesenteron epithelium and reproductive organs of female cat fleas, ctenocephalides felis bouché (siphonaptera: pulicidae), are related to the degree of reproductive maturation or regression. contrary to previous ideas, blue bodies in the ovarioles are degenerate oocyte nuclei and their presence denotes failure of ripening oocytes to reach full maturity. a distinction between true corpora lutea and pseudo-corpora lutea is established, the presence of the former ...19911768929
effect of larval diet on cat flea (siphonaptera: pulicidae) developmental times and adult emergence.the natural diet of cat flea, ctenocephalides felis (bouche), larvae is primarily adult flea feces, but dried bovine blood may be substituted in the laboratory. percentage adult emergence (79.4% on feces; 78.9% on blood) and developmental times (20.6 d on feces; 17.1 d on blood) did not significantly differ for the two diets. the drying temperature of blood determined its quality; blood dried at 120 degrees c was unsatisfactory for larval development. the dietary value of dried bovine blood was ...19911842797
blood consumption by the cat flea, ctenocephalides felis (siphonaptera: pulicidae).the volume of blood consumed by actively reproducing female cat fleas, ctenocephalides felis (bouché), while feeding in confinement feeding chambers on cats for 3 h, was investigated using two radionuclide blood tags (51cr-erythrocyte and 125i-albumin) and the gravimetric method. female fleas consumed an average of 0.110 ml (+/- 0.026 ml) of blood per 100 females in 3 h as determined using the dual radionuclide system. the single 51cr-erythrocyte tag overestimated blood consumption by 11.3% and ...19911875365
effect of an experimental systemic compound, cga-184699, on life stages of the cat flea (siphonaptera: pulicidae).the experimental drug cga-184699 (n-[2,5-dichloro-4-(1,1,2,3,3,3-hexafluoropropoxy)-phenylaminoc arbonyl]-2,6-difluorobenzamide) was evaluated for efficacy against cat fleas, ctenocephalides felis (bouché), held in flea cages on cats. when administered orally, the compound acts systemically and prevents development of the next generation of fleas. there was no effect on adults, but eggs from adults that fed on treated cats had reduced viability. most larvae that emerged from surviving eggs died. ...19911875371
separation of cat flea (siphonaptera: pulicidae) instars by individual rearing and head width measurements.two methods to verify whether head width measurements fit dyar's rule were evaluated for the separation of instars of the cat flea, ctenocephalides felis (bouché). individual rearing was a reliable method of determining larval instar but was labor-intensive. the mean observed head widths were significantly different for each instar (first instar, 0.164 mm; second instar, 0.201 mm; third instar, 0.260 mm) and showed no sexual dimorphism. head capsule width increased roughly 25% from instar to ins ...19911885846
[the biology of dipylidium caninum. part 2].from 198 cats and 182 dogs in austria 9,134 fleas were collected. ctenocephalides felis is the main flea of our cats (98.5%) and dogs (77.5%). demonstration of cysticercoids of dipylidium caninum through bleaching of fleas failed. dissection of fleas gave, however, positive results. each 44th flea from cats and 61st flea from dogs harbours cysticercoids. infection intensity rates were 2.3% for c. felis (cats), 1.2% for c. felis (dogs), and 3.1% for c. canis (dogs). male fleas are more extensivel ...19911927147
[studies on esterase isoenzyme of three species of flea].the patterns of esterase (est) isoenzyme of 3 flea species were studied by vertical slab polyacrylamide gel electrophoresis (page). the samples prepared from the third larval instar, female and male (the unsucking and sucked one) were run in the same gel slab. the result was as follows: xenopsylla cheopis showed 14 bands, with 5-6 major bands, ctenocephalides felis felis showed 9 bands with 4-5 major bands and nosopsyllus laeviceps kuzenkovi showed 9 bands with 4-5 major bands. these three speci ...19911959176
mass flea outbreak at a child care facility: case report.cat fleas, ctenocephalides felis bouchet, were collected at a mississippi child care facility after reports of large numbers of adult fleas occurring on children and personnel. one building yielded 161 (99 percent) of the fleas collected. urticarial lesions due to flea bites occurred on the legs of six children. flea presence was due to cats occupying the crawl space. fleas were eradicated by eliminating entry of cats and using residual insecticides throughout the facility.19912003633
parasites, pets, and people.it is important for the family physician to understand that patients' relationships with their pets play an important role in helping maintain mental and physical health yet provide the potential for causing illness in the patient. toxocara canis (dog roundworm) and toxocara cati (cat roundworm) are the ascarids most commonly responsible for vlm and ocular larva migrans in humans. these roundworms live in their adult stage in the small intestine of the dog and cat where their eggs are passed in ...19912011635
isolation of borrelia burgdorferi from arthropods collected in texas.the texas department of health laboratory cultured arthropods from november 1988 through december 1989 in an attempt to isolate borrelia burgdorferi, the etiologic agent of lyme disease. spirochetes were isolated from eight of 1,093 pools of arthropods cultured. the spirochetal isolates were from several tick and one flea species, including amblyomma americanum, a. maculatum, ixodes scapularis, and ctenocephalides felis. these 8 isolates reacted specifically when treated with monoclonal antibodi ...19912063950
[ctenocephalides canis and ctenocephalides felis infestations of dogs and cats. biology of the agent, epizootiology, pathogenesis, clinical signs, diagnosis and control].a review is presented of the biology, epizootiology, pathogenesis, clinical features, diagnosis and treatment of canine and feline ctenocephalides canis and c. felis infestation. also data are given on the prevalence of fleas in dogs and cats during a year at the 1st animal medical hospital, munich veterinary faculty. 5.5% of the dogs and 18.9% of the cats were infested with fleas.19912068719
circadian rhythm in the cat flea, ctenocephalides felis (siphonaptera: pulicidae). 19902217357
ovicidal and larvicidal modes of action of fenoxycarb against the cat flea (siphonaptera: pulicidae).the mode of action of the insect growth regulator, fenoxycarb, on embryogenesis of cat flea eggs, ctenocephalides felis (bouché), was evaluated biologically and morphologically. newly oviposited flea eggs were aged for 0, 3, 6, 12, 24, 48, or 72 h and exposed to fenoxycarb (1.1 micrograms/cm2) on filter paper disks for 0 (60 s), 2, 4, 8, 12, or 24 h at 23 +/- 1 degree c and 70 +/- 3% rh. following exposure, samples of flea eggs were processed for microscopic examination and seeded onto carpet sw ...19902231627
infection of colonized cat fleas, ctenocephalides felis (bouché), with a rickettsia-like microorganism.we report the ultrastructure of a rickettsia-like microorganism in a colonized population of the cat flea ctenocephalides felis (bouché). the microorganism occurs principally in the cytoplasm of midgut cells, but similar microorganisms were detected in the tracheal matrix, muscle, hypodermis, ovaries, and the epithelial sheath of the testes. the microorganism has a well-defined cell membrane consistent with rickettsia and measures 0.25-0.45 microns in diameter with lengths up to 1.5 microns. it ...19902240368
egg production, larval development, and adult longevity of cat fleas (siphonaptera: pulicidae) exposed to ultrasound.adult cat fleas, ctenocephalides felis felis (bouché), on cats (felis catus) were exposed to emissions from an ultrasonic flea collar worn by the cat. no significant differences were found in total numbers of eggs produced per day (mean = 524 control, 614 treatment), in length of larval development time (mean = 7.7 d control, 7.7 d treatment), or in total daily pupal production (mean = 485 control, 445 treatment) between the treatment and the control groups. tests off the host were conducted to ...19902280050
field evaluation of nylar for control of cat fleas (siphonaptera: pulicidae) in home yards.procedures were developed to evaluate juvenoid insect growth regulators in home yards as part of an ongoing program to identify photostable insecticides for control of the cat flea, ctenocephalides felis (bouché). nylar, an emulsifiable concentrate formulation of pyriproxyfen, was selected as the experimental insect growth regulator. efficacy studies, yard survey, and pretreatment sampling techniques are described. soil samples collected from the yards at intervals after treatment were taken to ...19902280389
prevalence and biology of endosymbionts of fleas (siphonaptera: pulicidae) from dogs and cats in alachua county, florida.a study was conducted to determine the prevalence and biology of endosymbionts in local populations of fleas collected from dogs and cats in alachua co., florida. four hundred three ctenocephalides felis (bouché), 194 pulex simulans baker, and 44 echidnophaga gallinacea (westwood) were examined. fleas were collected from 52 dogs and 51 cats. from 1 to 20 fleas were dissected from each host. a variety of microorganisms and metazoa was observed, including a baculovirus, gram-negative bacteria, ric ...19902280390
nolleria pulicis n. gen., n. sp. (microsporida: chytridiopsidae), a microsporidian parasite of the cat flea, ctenocephalides felis (siphonaptera: pulicidae).a new species of microsporidium, nolleria pulicis, is described and named here from the cat flea, ctenocephalides felis. the genus nolleria is created and placed within the family chytridiopsidae. the family is slightly modified to accommodate certain features of intracellular development seen in n. pulicis, which is otherwise very similar to other species in the family chytridiopsidae. sporulation is described from ultrastructural analysis of infected midgut epithelial cells of adult c. felis. ...19902319492
rickettsia in texas.since the first reported case in 1941, rocky mountain spotted fever in humans has been reported from many areas of texas, with two major foci, one located in the north-central region and the other in the eastern region of the state. during the period 1979-1988, 421 cases of rmsf were reported, reaching 108 cases in 1983 and declining in subsequent years. statewide surveillance programs to detect spotted fever group rickettsiae in tick populations were initiated in 1976. in recent years, the sfg ...19902378448
ectoparasitic and phoretic arthropods of virginia opossums (didelphis virginiana) in central tennessee.thirteen species of ectoparasitic (12) or phoretic (1) arthropods were collected from 26 adult virginia opossums, didelphis virginiana, live-trapped from april through september 1987 in davidson county, tennessee. the cat flea ctenocephalides felis and the american dog tick dermacentor variabilis were the predominant species with respect to mean intensity and prevalence. the macronyssid mite ornithonyssus wernecki and the atopomelid mite didelphilichus serrifer, both specific parasites of this h ...19902380870
detection of murine typhus infected fleas with an enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay (elisa) for the detection of rickettsia typhi antigen in homogenates of pooled or individual laboratory infected fleas is described. the assay uses a double sandwich technique, employing a pool of monoclonal antibodies to capture the antigen and a hyperimmune rabbit serum for antigen detection. using pools of r. typhi infected xenopsylla cheopis, ctenocephalides felis, and leptopsylla segnis, the sensitivity of the elisa was compared with direct fluorescent a ...19892499204
host association, on-host longevity and egg production of ctenocephalides felis felis.host association, on-host longevity and egg production of ctenocephalides felis felis (bouché) were evaluated using fleas from a commercial laboratory colony and first generation, laboratory-reared, native indiana fleas. fleas were placed on cats that were declawed, fitted with elizabethan collars and housed in specially designed metabolic cages. an average of 85% of the female and 58% of the male fleas stayed continuously on the cats for at least 50 days, indicating that the cat flea is a perma ...19892588462
problems of housing and health of people utilizing the garbage in cairo from the viewpoint of medical entomology.more than 50 arthropod species were found at two dumping-grounds with dwelling-places (mokattam and ezbet el nakhl) in cairo. their importance was evaluated from the epidemiological and epizootological points of view. of primary importance is the great prevalence of the fleas (xenopsylla cheopis) on brown rats (rattus norvegicus), since the rats are in a close contact with people. they are often simultaneously parasitized with another flea species, ctenocephalides felis felis, which is very freq ...19892634080
effects of ultrasonic flea collars on ctenocephalides felis on cats.ultrasonic flea collars marketed by 2 companies were evaluated for their ability to reduce flea numbers on cats with experimentally induced flea (ctenocephalides felis) infestations. the sound output of the collars was evaluated both before and after use to ensure that the collars were functional. each brand was evaluated on 5 cats for a 7-day period. collars generated peak frequencies of 40 khz and 80 to 92 db sound pressure level at 10 cm. an average of 98.6 and 97.4% of the fleas were still o ...19892641212
circadian rhythm of cat flea (siphonaptera: pulicidae) locomotion unaffected by ultrasound.cat fleas, ctenocephalides felis (bouché), had a circadian rhythm of locomotion that peaked during the last 2 h of the photophase and declined to a lower level that was maintained throughout the scotophase. activity was lowest during the first 8 h of photophase. the circadian rhythm of these fleas was not affected by an ultrasonic pest control device.19892708627
mortality in calves, lambs and kids caused by severe infestation with the cat flea ctenocephalides felis felis (bouché, 1835) in israel.heavy infestation of calves, lambs and kids with the cat flea ctenocephalides felis felis (bouché, 1835), accompanied by severe anaemia and mortality, is described. lambs and kids were affected more severely than calves. flea infestation was more widespread in summer and autumn than in winter and spring. the clinical findings are discussed in the light of the pertinent literature.19892728326
[flea infestation establishment in the east german district of rostock].from sendings of citizens and own examinations 9 species of fleas were found out. ctenocephalides felis was the most frequent species, followed by ceratophyllus gallinae and pulex irritans. most of the sendings came from cities. pests and flea control dominated in the months august to october.19892729648
susceptibility of the cat flea (siphonaptera: pulicidae) to pyrethroids.adult cat fleas, ctenocephalides felis felis (bouché), from two laboratory colonies (one originating in california and one from florida) were exposed to residues of eight pyrethroids to compare their susceptibilities. the florida strain was more tolerant than the california strain, with 6.8-, 5.2-, and 4.8-fold tolerance to cyfluthrin, cypermethrin, and fluvalinate, respectively. the florida strain showed less than 3-fold tolerance to the other five insecticides (permethrin, tralomethrin, d-phen ...19892754097
activity of insecticides against the preemerged adult cat flea in the cocoon (siphonaptera: pulicidae)].the activity of insecticides against the adult cat flea, ctenocephalides felis (bouché), inside the cocoon (preemerged) was determined by spraying the cocoons directly and observing subsequent emergence of adults over the next 14 d. direct sprays of 0.5% trimethacarb wettable powder (wp) and chlorpyrifos emulsifiable concentrate (ec) and wp provided greater than 70% kill. the order of activity against the preemerged adult exposed to direct sprays was organophosphates greater than carbamates grea ...19892769709
observations on the susceptibility of ctenocephalides felis (siphonaptera: pulicidae) to malathion and permethrin in tanzania.laboratory-reared ctenocephalides felis (bouche) adults were tested with 0.5% malathion and 0.5% permethrin, using the standard who methods. after 24 h exposure to malathion (3.6 mg/cm2), 92% of the fleas died. the lt50 for malathion was approximately 8 h. permethrin (0.45 mg/cm2) produced 100% mortality of exposed insects after 24 h while with a higher dose (0.9 mg/cm2) all fleas died after 8 h exposure. lt50 for the two doses of permethrin were 7.7 and 1.05 h, respectively. the failure of the ...19882980190
ectoparasites of guinea fowl (numida meleagris galeata pallas) and local domestic chicken (gallus gallus) in southern guinea savanna, nigeria.the ectoparasites of poultry in a southern guinea savanna zone were investigated by the examination of guinea fowl and local domestic chickens in the range and guinea fowl under intensive management. the prevalent ectoparasites of range guinea fowl and local chickens include seven species of lice menacanthus stramineus, menopon gallinae, goniodes gigas, goniocotes gallinae, lipeurus caponis, numidilipeurus tropicalis, damalinia bovis; three mites bdellonyssus bursa, megninia cubitalis, dermanyss ...19883195043
[study of siphonaptera in somalia].in the period 1982-1984 samples of fleas were collected from wild animals of the middle scebeli, low scebeli and bay regions of somalia. in total 1,335 specimens (486 males and 849 females) were obtained from 17 species of mammalian hosts out of the 19 examined. the following species of fleas were identified: echidnophaga gallinacea, e. larina, e. murina, ctenocephalides felis strongylus, synosternus burtoni, s. somalicus, s. burtoni, s. somalicus, c. felis strongylus and e. larina are known to ...19883271983
performance of insecticides for control of cat fleas (siphonaptera: pulicidae) indoors. 19883351080
lack of behavioral responses of the cat flea, ctenocephalides felis (siphonaptera: pulicidae), to a broad spectrum of ultrasound. 19883351872
toxicity of linalool to life stages of the cat flea, ctenocephalides felis (siphonaptera: pulicidae), and its efficacy in carpet and on animals. 19883357170
survival and reproduction of artificially fed cat fleas, ctenocephalides felis bouché (siphonaptera: pulicidae). 19883392715
aspects of the immunopathogenesis of flea allergy dermatitis in dogs.this paper reviews the current data on the nature of the allergen(s) of the cat flea, ctenocephalides felis and on some of the factors that determine the development and maintenance of the allergic state. the role of immediate hypersensitivity mediated by ige, delayed hypersensitivity and of cutaneous basophil hypersensitivity in the immunopathogenesis is discussed. finally, data on the nature of immunologic non-reactivity is presented and the prospects for therapeutic immunologic intervention d ...19873433670
flea species from dogs and cats in north-central florida.fleas were identified after removal from 100 dogs and 60 cats following routine presentation at a veterinary hospital in gainesville, fl. ctenocephalides felis comprised 92.4% and 99.8% of the fleas taken from dogs and cats respectively. pulex irritans accounted for 7.5% of the fleas found on dogs and for 0.2% of those found on cats. echidnophagia gallinacia was found on one dog. of the 100 dogs, 80 were infested with c. felis alone, one with p. irritans alone, and 18 carried a mixed infestation ...19873564340
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