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effects of meloidogyne incognita on growth and storage-root formation of cassava (manihot esculenta).two-node cuttings of cassava cultivar ss4 were inoculated with 1,000 infective juveniles of meloidogyne incognita at 1, 14, 40, 70, 88, and 127 days after planting (dap). plant growth and root damage were assessed at 150 dap. meloidogyne incognita significantly reduced the number of storageroots formed in plants inoculated at 14, 40, 70, and 88 dap and the total weight of storage-roots in plants inoculated at 1, 14, 40, 70, and 88 dap, compared to uninoculated plants. individual storage-root wei ...200019270997
heat stability of resistance to meloidogyne incognita in scotch bonnet peppers ( capsicum chinense jacq.).stability of resistance to meloidogyne incognita (kofoid &white) chitwood was determined in pepper (capsicum chinense jacq. and c. annuum l.) at 24, 28, and 32 degrees c. reactions of the c. annuum cultivars charleston belle and keystone resistant giant and the c. chinense cultigens pa-426 and pa-350 to m. incognita were compared. charleston belle is homozygous for the n gene that confers resistance to m. incognita in c. annuum, and keystone resistant giant is the susceptible recurrent parent of ...200019270989
using microwave irradiation to improve preservation of female nematodes and gall tissues for tem observations.microwave irradiation of glutaraldehyde-immersed samples was evaluated for the chemical fixation of 3-week-old galls that resulted from the infection of tomato roots (lycopersicon esculentum) by a root-knot nematode, meloidogyne incognita. observation by transmission electron microscopy indicated that the best results were obtained when vials containing the intact galls were immersed in buffered glutaraldehyde and irradiated for 10 seconds then allowed to cool for 30 seconds; this procedure was ...200019270984
variability in time and space of meloidogyne incognita fall population density in cotton fields.three cotton fields infested with meloidogyne incognita were intensively sampled in the fall for 3 years (1996 to 1998) to determine if intensive sampling for m. incognita, for which spatial location is important, was necessary every year in a continuous cotton system. two composite soil samples (20 cores each), taken over an area covering one-third of the field length and two rows wide, were averaged to represent that area (row-location combination). each field (except one) had 24 areas assayed ...200019270975
nematicidal constituents of the aerial parts of lantana camara.two new constituents, lantanoside (1) and lantanone (2), and the known compounds linaroside (3) and camarinic acid (4) were isolated from the aerial parts of lantana camara. compounds 1, 3, and 4 were tested for nematicidal activity against root-knot nematode meloidogyne incognita and showed 90, 85, and 100% mortality, respectively, at 1.0% concentration. the results were comparable to those obtained with the conventional nematicide furadan (100% mortality at 1.0% concentration). structures of t ...200010869197
origin of a meloidogyne incognita surface coat antigen.the surface coat (sc) of plant nematodes is thought to originate either from the living hypodermis or from secretory glands associated with the excretory system or nervous system. in this study, we investigated the origin of the sc of meloidogyne incognita by immunolocalization with a monoclonal antibody raised against the surface coat of the preparasitic juveniles (j2). under the electron microscope, strong labeling was found on the cuticular surface and in the rectal dilation of the j2, while ...200019270963
effect of mulch surface color on root-knot of tomato grown in simulated planting beds.the effect of different-colored polyethylene mulches on quantity and spectra of reflected light, plant morphology, and root-knot disease was studied in tomato (lycopersicon esculentum) grown in simulated planting beds. tomato plants were inoculated with meloidogyne incognita at initial populations (pi) of 0, 1,000, 10,000, or 50,000 eggs/plant, and grown in a greenhouse for 50 days over white, red, or black mulch. soil temperature was kept constant among the mulch treatments by placing an insula ...200019270954
species-dependent effects of border cell and root tip exudates on nematode behavior.abstract effects of border cell and root tip exudates on root knot nematode (meloidogyne incognita) behavior were examined. in whole-plant assays using pea, m. incognita second-stage juveniles (j2) accumulated rapidly around the 1- to 2-mm apical region ensheathed by border cells, but not in the region of elongation. within 15 to 30 min, j2 which had accumulated within detached clumps of border cells lost motility and entered into a quiescent state. when border cells (and associated root tip exu ...200018944426
effect of temperature on and histopathology of the interaction between meloidogyne incognita and thielaviopsis basicola on cotton.abstract controlled environments were used to study the relationship between the root-knot nematode (meloidogyne incognita) and thielaviopsis basicola on cotton. temperature treatments were continuous 20, 24, and 28 degrees c or two cyclic linear regimes with ranges of 14 to 32 or 18 to 28 degrees c over 24 h. cotton seeds were planted in fumigated soil infested with t. basicola, m. incognita, or both. after 42 days, pathogen effects on plant growth and pathogen development were evaluated. histo ...199918944671
comparison of variable and single-rate applications of aldicarb on cotton yield in fields infested with meloidogyne incognita.variable-rate applications of the nematicide aldicarb were compared to producer standard rates in eight field tests over 3 years. test areas (308 to 1,015 m long) were divided into eight or five blocks. each block contained two plots with a variable-rate treatment (vrt) of aldicarb and a producer standard treatment (pst) of aldicarb. each vrt plot was divided into three subunits and intensively sampled for meloidogyne incognita in either the fall or spring before planting. rates of aldicarb were ...199919270939
evaluation of dry ice as a potential cryonematicide for meloidogyne incognita in soil.solid co (dry ice) was added to pots containing soil that was infested either with eggs of the root-knot nematode, meloidogyne incognita, or with tomato (lycopersicon esculentum 'rutgers') root fragments that were infected with various stages of the nematode. two hours after dry ice was added, thermocouples in the soil recorded temperatures ranging from -15 degrees c to -59 degrees c. one day after treatment with the dry ice, the temperature of the soil was allowed to equilibrate with that of th ...199919270918
isolation of a cdna encoding a beta-1,4-endoglucanase in the root-knot nematode meloidogyne incognita and expression analysis during plant parasitism.a beta-1,4-endoglucanase encoding cdna (egases, e.c. 3.2.1.4), named mi-eng-1, was cloned from meloidogyne incognita second-stage juveniles (j2). the deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. in m. incognita, the gene is transcribed in the migratory j2, in males, and in the sedentary adult females. in pre-parasitic j2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. the presence of bet ...199910478479
carbon partitioning in soybean infected with meloidogyne incognita and m. javanica.seven-day-old seedlings of two cultivars (cristalina and ufv itm1) of glycine max were inoculated with 0, 3,000, 9,000, or 27,000 eggs of meloidogyne incognita race 3 or m. javanica and maintained in a greenhouse. thirty days later, plants were exposed to (1)co for 4 hours. twenty hours after (1)co exposure, the root fresh weight, leaf dry weight, nematode eggs per gram of root, total and specific radioactivity of carbohydrates in roots, and root carbohydrate content were evaluated. meloidogyne ...199919270907
reproduction and development of meloidogyne incognita and m. javanica on guardian peach rootstock.guardian peach rootstock was evaluated for susceptibility to meloidogyne incognita race 3 (georgia-peach isolate) and m. javanica in the greenhouse. both commercial guardian seed sources produced plants that were poor hosts of m. incognita and m. javanica. reproduction as measured by number of egg masses and eggs per plant, eggs per egg mass, and eggs per gram of root were a better measure of host resistance than number of root galls per plant. penetration, development, and reproduction of m. in ...199919270905
meloidogyne incognita surface antigen epitopes in infected arabidopsis roots.surface-coat epitopes of meloidogyne incognita were detected in root tissues of arabidopsis thaliana during migration and feeding site formation. a whole-mount root technique was used for immunolocalization of surface coat epitopes in a. thaliana, with the aid of a monoclonal antibody raised specifically against the outer surface of infective juveniles of m. incognita. the antibody, which was meloidogyne-specific, recognized a fucosyl-bearing glycoprotein in the surface coat. during migration in ...199919270892
effects of a resistant corn hybrid and fenamiphos on meloidogyne incognita in a corn-squash rotation.the efficacy of a double-cross corn (zea mays) hybrid (old raccoon selection x t216) x (tebeau selection x mp 307) resistant to meloidogyne incognita as a rotational crop, and fenamiphos treatment for management of root-knot nematode (m. incognita race 1) in squash (cucurbita pepo var. melopepo) was evaluated in field tests during 1996 and 1997. numbers of m. incognita in the soil and root-gall indices were lower on the resistant hybrid than on a commercial cultivar dekalb dk-683. treatment mean ...199919270888
rhizosphere colonization and control of meloidogyne spp. by nematode-trapping fungi.the ability of nematode-trapping fungi to colonize the rhizosphere of crop plants has been suggested to be an important factor in biological control of root-infecting nematodes. in this study, rhizosphere colonization was evaluated for 38 isolates of nematode-trapping fungi representing 11 species. in an initial screen, arthrobotrys dactyloides, a. superba, and monacrosporium ellipsosporum were most frequently detected in the tomato rhizosphere. in subsequent pot experiments these fungi and the ...199919270886
molecular cloning of an acetylcholinesterase gene from the plant parasitic nematodes, meloidogyne incognita and meloidogyne javanica.a gene encoding a protein with strong homology with caenorhabditis elegans and c. briggsae acetylcholinesterase ace-1 was cloned from meloidogyne incognita and m. javanica pre-parasitic juveniles. both cdnas have an orf of 1968 bp for a deduced translation product of 656 amino acid residues. the key residues essential to acetylcholinesterase (ache) structure and function are conserved in both sequences. m. incognita and m. javanica ache share a homology of 98.8% at the amino acid level and 97% a ...199910340488
isolation of the lemmi9 gene and promoter analysis during a compatible plant-nematode interaction.plant-endoparasitic root-knot nematodes feed on specialized giant cells that they induce in the vascular cylinder of susceptible plants. although it has been established that a number of plant genes change their expression pattern during giant cell differentiation, virtually no data are available about the mechanisms involved in that change. one possibility is differential promoter recognition by the transcription factor(s) responsible for the expression of specific genes. we have isolated and c ...199910226377
greenhouse studies on the effect of marigolds (tagetes spp.) on four meloidogyne species.the effects of preplanted marigold on tomato root galling and multiplication of meloidogyne incognita, m. javanica, m. arenaria, and m. hapla were studied. marigold cultivars of tagetes patula, t. erecta, t. signata, and a tagetes hybrid all reduced galling and numbers of second-stage juveniles in subsequent tomato compared to the tomato-tomato control. all four meloidogyne spp. reproduced on t. signata 'tangerine gem'. several cultivars of t. patula and t. erecta suppressed galling and reproduc ...199919270876
in-vitro assays of meloidogyne incognita and heterodera glycines for detection of nematode-antagonistic fungal compounds.in-vitro methods were developed to test fungi for production of metabolites affecting nematode egg hatch and mobility of second-stage juveniles. separate assays were developed for two nematodes: root-knot nematode (meloidogyne incognita) and soybean cyst nematode (heterodera glycines). for egg hatch to be successfully assayed, eggs must first be surface-disinfested to avoid the confounding effects of incidental microbial growth facilitated by the fungal culture medium. sodium hypochlorite was mo ...199919270887
identification of sources of resistance to four species of root-knot nematodes in tobacco.resistance to the southern root-knot nematode, meloidogyne incognita races 1 and 3, has been identified, incorporated, and deployed into commercial cultivars of tobacco, nicotiana tabacum. cultivars with resistance to other economically important root-knot nematode species attacking tobacco, m. arenaria, m. hapla, m. javanica, and other host-specific races of m. incognita, are not available in the united states. twenty-eight tobacco genotypes of diverse origin and two standard cultivars, nc 2326 ...199919270897
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
fosthiazate controls meloidogyne arenaria and m. incognita in flue-cured tobacco.the nematicide fosthiazate was evaluated over a 3-year period for management of meloidogyne incognita race 3 (site 1) and m. arenaria race 2 (site 2) in flue-cured tobacco. fosthiazate was applied broadcast and incorporated at rates ranging from 22 to 88 g a.i./100 m(2), and compared with the nematicides fenamiphos (67 g a.i./100 m(2)), 1,3-d (56.1 l/ha, 670 ml/100-m row), and an untreated control. root-gall indices and leaf yields were averaged over the 3-year period. root galling was negativel ...199919270938
effect of temperature on suppression of meloidogyne incognita by tagetes cultivars.the suppression of meloidogyne incognita by marigolds differed among six marigold cultivars and five soil temperatures. tagetes signata (syn. t. tenuifolia) cv. tangerine gem and the tagetes hybrid polynema allowed reproduction and root galling when grown at 30 degrees c, and should not be used for control of m. incognita at temperatures close to 30 degrees c. tagetes patula cultivars single gold and tangerine and t. erecta flor de muerto, when grown within a 20-30 degrees c soil temperature ran ...199919270940
identification and characterization of excreted-secreted products and surface coat antigens of animal and plant-parasitic nematodes.nematode surface coat (sc) proteins and excreted-secreted products (e-s) are likely to play important roles in the host-parasite interaction and considerable similarities can be found in sc proteins and e-s products from certain plant and animal parasitic nematodes. monoclonal antibodies raised to e-s products of plant-parasitic nematodes were shown to cross-react with e-s products and the surface coats of the animal parasites trichinella spiralis and haemonchus contortus. most of the antibodies ...199910340331
differential in vitro pathogenicity of predatory fungi of the genus monacrosporium for phytonematodes, free-living nematodes and parasitic nematodes of cattle.in vitro tests were carried out on the pathogenicity of nine isolates of the predatory fungi of the genus monacrosporium (5 m. sinense isolates, 3 m. appendiculatum and 1 m. thaumasium isolate) for a phytonematode (second stage juveniles from meloidogyne incognita, race 3), a free-living nematode (panagrellus spp), and two gastrointestinal parasitic nematodes of cattle (infective larvae of cooperia punctata and haemonchus placei). a suspension containing 2,000 nematodes from each species was add ...199910347773
host suitability of potential cover crops for root-knot nematodes.several potential cover crops were evaluated for their susceptibility to meloidogyne arenaria race 1, m. incognita race 1, and m. javanica in a series of five greenhouse experiments. no galls or egg masses were observed on roots of castor (ricinus communis), cowpea (vigna unguiculata cv. iron clay), crotalaria (crotalaria spectabilis), or american jointvetch (aeschynomene americana). occasional egg masses (rating </=1.0 on 0-5 scale) were observed on marigold (tagetes minuta) in one test with m. ...199919270926
host status of herbaceous perennials to meloidogyne incognita and m. arenaria.twenty-two different herbaceous perennials were studied for their reaction to separate inoculations of meloidogyne arenaria and m. incognita under greenhouse conditions. perennial taxa that did not develop root-galls following inoculation, and therefore are considered as nonhosts of both nematode species, included species and cultivars of aethionema, fragaria, phlox, and polygonum. echinacea, monarda, and patrinia developed only a few galls. root-galls developed on species and cultivars of achil ...199819274254
a secretory cellulose-binding protein cdna cloned from the root-knot nematode (meloidogyne incognita).a cdna encoding a secretory cellulose-binding protein was cloned from the root-knot nematode (meloidogyne incognita) with rna fingerprinting. the putative full-length cdna, named mi-cpb-1, encoded a 203 amino acid protein containing an n-terminal secretion signal peptide. the c-terminal sequence of the putative mi-cbp-1 was similar to a bacterial-type cellulose-binding domain, whereas the n-terminal sequence did not show significant similarity to any proteins in data bases. recombinant mi-cbp-1 ...19989768512
pathogenicity of pratylenchus penetrans, heterodera glycines, and meloidogyne incognita on soybean genotypes.the pathogenicity of heterodera glycines, meloidogyne incognita, and pratylenchus penetrans on h. glycines-resistant 'bryan,' tolerant-susceptible 'g88-20092,' and intolerant-susceptible 'tracy m' soybean cultivars was tested using plants grown in 800 cm(3) of soil in 15-cm-diam. clay pots in three greenhouse experiments. plants were inoculated with 0, 1,000, 3,000, or 9,000 h. glycines race 3 or m. incognita eggs, or vermiform stages of p. penetrans/pot. forty days after inoculation, nmnbers of ...199819274203
the potential of thiarubrine c as a nematicidal agent against plant- parasitic nematodes.thiarubrine c, a polyacetylenic 1,2-dithiin isolated from the roots of rudbeckia hirta (asteraceae), exhibited strong nematicidal activity in in vitro and growth chamber assays. thiarubrine c was toxic, in the absence of light, to the plant-parasitic nematodes meloidogyne incognita and pratylenchus penetrans at lcs of 12.4 ppm and 23.5 ppm, respectively. a minimum exposure time between 12 and 24 hours was the critical period for nematode mortality due to thiarubrine c. although thiarubrine c was ...199819274210
peanut-cotton-rye rotations and soil chemical treatment for managing nematodes and thrips.in the southeastern united states, a cotton-peanut rotation is attractive because of the high value and extensive planting of both crops in the region. the objective of this experiment was to determine the effects of cotton-peanut rotations, rye, and soil chemical treatments on management of plant-parasitic nematodes, thrips, and soilborne fungal diseases and on crop yield. peanut-cotton-rye rotations were conducted from 1988 to 1994 on tifton loamy sand (plinthic kandiudult) infested primarily ...199819274213
caenorhabditis elegans: a genetic guide to parasitic nematode biology.the advent of parasite genome sequencing projects, as well as an increase in biology-directed gene discovery, promises to reveal genes encoding many of the key molecules required for nematode-host interactions. however, distinguishing parasitism genes from those merely required for nematode viability remains a substantial challenge. although this will ultimately require a functional test in the host or parasite, the free-living nematode caenorhabditis elegans can be exploited as a heterologous s ...199819274223
crop yields and nematode population densities in triticale-cotton and triticale-soybean rotations.triticale cv. beagle 82, cotton cv. mcnair 235, and soybean cv. twiggs were arranged in three cropping sequences to determine the effects of fenamiphos and cropping sequence on nematode population densities and crop yields under conservation tillage for 4 years. the cropping sequences were triticale (t)-cotton (c)-t-c, t-soybean (s)-t-s, and t-c-t-s. numbers of meloidogyne incognita second-stage juveniles declined on trificale but increased on cotton and soybean each year. root-gall indices of c ...199819274228
resistance to root-knot, reniform, and soybean cyst nematodes in selected soybean breeding lines.soybean breeding lines and reported sources of nematode resistance were evaluated in repeated greenhouse tests for resistance to north carolina populations of the soybean cyst nematode heterodera glycines, reniform nematode rotylenchulus reniformis, and the root-knot nematode species meloidogyne incognita, m. arenaria, and m. arenaria. lines from the soybean breeding program in missouri that had 'hartwig' soybean as a parent were the most resistant to races 1-4 of the soybean cyst nematode and t ...199819274243
survey of heterodera glycines races and other plant-parasitic nematodes on soybean in north carolina.a survey of soybean-production areas in the piedmont, coastal plain and tidewater regions of north carolina was conducted from 1994 to 1996. heterodera glycines was detected in 55 of 77 fields sampled in 15 counties. the host race of h. glycines was determined for 39 of the populations collected. of all populations collected, 4% were race 1, 40% race 2, 16% race 4, 7% race 5, and 4% race 9; the remaining 29% could not be accurately categorized. none of the populations evaluated had high levels o ...199819274248
the tomato mi-1 gene confers resistance to both root-knot nematodes and potato aphids.mi-1, a lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode meloidogyne incognita and to an unrelated pathogen, the potato aphid macrosiphum euphorbiae. this resis ...19989853621
rpe, a plant gene involved in early developmental steps of nematode feeding cells.sedentary plant-parasitic nematodes are able to induce the redifferentiation of root cells into multinucleate nematode feeding sites (nfss). we have isolated by promoter trapping an arabidopsis thaliana gene that is essential for the early steps of nfs formation induced by the root-knot nematode meloidogyne incognita. its pattern of expression is similar to that of key regulators of the cell cycle, but it is not observed with the cyst nematode. later in nfs development, this gene is induced by b ...19989843485
responses of cotton yield and meloidogyne incognita soil populations to soil applications of aldicarb and 1,3-d in florida.in four tests conducted in loamy-sand soils in northern florida, cotton lint yield increased and post-harvest soil populations of meloidogyne incognita were more effectively suppressed by 1,3-dichloropropene (1,3-d) than aldicarb when both nematicides were evaluated over a range of recommended rates. significant positive relationships existed between lint yield and rates of 1,3-d in three tests, whereas only one significant positive relationship occurred between lint yield and aldicarb rates. yi ...199819274259
response of sesamum indicum and s. radiatum accessions to root-knot nematode, meloidogyne incognita.twenty sesame indicum and four s. radiatum accessions in the usda plant introduction collection were evaluated for reaction to the root-knot nematode, meloidogyne incognita race 3, at two initial egg densities under greenhouse conditions. all sesame accessions produced considerably fewer root galls than the tomato cultivar rutgers. gall numbers varied slightly among accessions at the higher infestation density with even less variation at the lower density. egg mass indices indicated little repro ...199819274255
interaction between meloidogyne incognita and thielaviopsis basicola on cotton (gossypium hirsutum).the effects of meloidogyne incognita and thielaviopsis basicola on the growth of cotton (gossypium hirsutum) and the effects of t. basicola on m. incognita populations were evaluated in a 2-year study. microplots were infested with m. incognita, t. basicola, or a combination of m. incognita and t. basicola. uninfested plots served as controls both years. seedling survival was decreased by the m. incognita + t. basicola treatment compared to the control. meloidogyne incognita alone and m. incogni ...199819274234
identification of a meloidogyne incognita cuticle collagen gene and characterization of the developmental expression of three collagen genes in parasitic stages. 19989662034
reducing meloidogyne incognita injury to cucumber in a tomato-cucumber double-cropping system.the effects of a root-knot nematode-resistant tomato cultivar and application of the nematicide ethoprop on root-knot nematode injury to cucumber were compared in a tomato-cucumber double-cropping system. a root-knot nematode-resistant tomato cultivar, celebrity, and a susceptible cultivar, heatwave, were grown in rotation with cucumber in 1995 and 1996. celebrity suppressed populations of meloidogyne incognita in the soil and resulted in a low root-gall rating on the subsequent cucumber crop. n ...199819274214
potential of foliar, dip, and injection applications of avermectins for control of plant-parasitic nematodes.studies were conducted to determine the potential of two avermectin compounds, abamectin and emamectin benzoate, for controlling plant-parasitic nematodes when applied by three methods: foliar spray, root dip, and pseudostem injection. experiments were conducted against meloidogyne incognita on tomato, m. javanica on banana, and radopholus similis on banana. foliar applications of both avermectins to banana and tomato were not effective for controlling any of the nematodes evaluated. root dips o ...199819274200
degradation of fenamiphos in agricultural production soil.nematicides are used to control a wide variety of nematodes on many crops; unfortunately, oftentimes the control they provide is erratic. this erratic behavior is not always predictable and has been associated with chemical, physical, and biological degradation of nematicides. their accelerated degradation is an agricultural problem that has been observed in crop monocultures and in other crop production systems where a biodegradable compound is repeatedly applied to the same soil. the problem c ...199819274197
effect of compost and maize cultivars on plant-parasitic nematodes.effects of yard waste compost and maize (zea mays) cultivar on population densities of plant-parasitic nematodes were examined in four experiments in north florida. in one experiment, eight maize cultivars were evaluated; the other three experiments involved split-plot designs with compost treatments as main plots and maize cultivars as subplots. the three compost treatments used in these experiments were: 269 mt/ha of a yard-waste compost applied to the soil surface as a mulch, 269 mt/ha of com ...199719274277
impact of meloidogyne incognita on the incidence of peach tree short life in the presence of criconemella xenoplax.the relationship between cricenemella xenoplax alone and in combination with meloidogyne incognitaon the incidence of peach tree short life disease was studied in field microplots during 1989-96. the presence of m. incognita suppressed the population density of c. xenoplax on lovell peach. tree trunk diameter was significantly reduced in the presence of both nematode species prior to 1993. soil ph was lowest in the co-infection treatment as compared with the uninoculated control on three of the ...199719274276
colored mulches affect yield of fresh-market tomato infected with meloidogyne incognita.the effects of different-colored polyethylene mulches on the quantity and spectra of reflected light, earliness of fruit set, fruit yield and quality, and root-knot disease were studied in field-grown, staked tomato (lycopersicon esculentum). white mulch reflected more photosynthetic light and a lower far-red-to-red ratio than red mulch, whereas black mulch reflected less than 5 percent of any color. soil temperatures and fruit yields were recorded for tomato plants inoculated with meloidogyne i ...199719274191
evaluation of nemx, a new cultivar of cotton with high resistance to meloidogyne incognita.the level of resistance to root-knot nematode, meloidogyne incognita, in nemx, a new cultivar of the acala-type upland cotton, was evaluated in relation to four resistant breeding lines (n6072, n8577, n901, and n903) and four susceptible cultivars (maxxa, sj2, royale, and prema). in growth pouch tests, an average of only 4 nematode egg masses was produced on roots of nemx or the resistant lines, compared to a significantly higher average of 21 on the susceptible cultivars. in pot tests, the nema ...199719274190
activity and differential induction of chitinase isozymes in soybean cultivars resistant or susceptible to root-knot nematodes.host physiological events in relation to infestation by parasitic nematodes are not well documented. soybean plant responses to meloidogyne incognita infestation were compared to resistant (bryan) and susceptible (brim) cultivars at 0, 1, 3, 10, 20, and 34 days after infestation (dai). the resistant cultivar had higher chitinase activity than the susceptible cultivar at every sample time beginning at 3 dai. results from isoelectric focusing gel electrophoresis analyses indicated that three acidi ...199719274189
meloidogyne incognita inoculum source affects host suitability and growth of yellow nutsedge and chile pepper.meloidogyne incognita (mi) reproduction and host plant responses in chile pepper (capsicum annuum) and yellow nutsedge (cyperus esculentus = yns) to three sources of inoculum obtained by rearing a single mi population on chile, yns, and tomato were evaluated in two factorial greenhouse experiments. the interactive effects of mi inoculum source and crop-weed competition were determined. in the absence of yns competition, chile growth was reduced less by mi inoculum from chile than by inoculum fro ...199719274174
evaluation of 15 trifolium spp. and of medicago sativa as hosts of four meloidogyne spp. found in new zealand.the predominant root-knot nematode in new zealand pastures is meloidogyne trifoliophila, identified until recently as m. hapla. clarification was needed on the host range of these two species on legumes found in new zealand pastures and on clover species closely related to trifolium repens. in a greenhouse test, 15 trifolium spp. and medicago sativa were inoculated with eggs of m. trifoliophila, m. hapla, m. incognita, or m. javanica. all legumes tested were hosts to some degree to each of the r ...199719274267
nematicidal activity of fatty acid esters on soybean cyst and root-knot nematodes.researchers have indicated that the c fatty acid, pelargonic acid (nonanoic acid), has considerable nematicidal activity that could be increased by derivitization and improved emulsification. microemulsions of methyl and ethylene glycol esters of pelargonic acid developed by mycogen corporation (san diego, ca) were tested for nematicidal activity against root-knot and soybean cyst nematodes. all treamaents were compared to a deionized water control and a microemulsion "blank" (minus active ingre ...199719274268
coastal bermudagrass rotation and fallow for management of nematodes and soilborne fungi on vegetable crops.the efficacy of clean fallow, bermudagrass (cynodon dactylon) as a rotational crop, and fenamiphos for control of root-knot nematode (meloidogyne incognita race 1) and soilborne fungi in okra (hibiscus esculentus), snapbean (phaseolus vulgaris), and pepper (capsicum annuum) production was evaluated in field tests from 1993 to 1995. numbers of m. incognita in the soil and root-gall indices were greater on okra than on snapbean or pepper. application of fenamiphos at 6.7 kg a.i./ha did not suppres ...199719274273
response of meloidogyne spp., heterodera glycines, and radopholus similis to tannic acid.tannins, which are water-soluble polyphenols, are toxic to numerous fungi, bacteria, and yeasts. our objectives were to study the efficacy of tannic acid in control of meloidogyne arenaria on tomato and its effects on the behavior of m. arenaria, m. incognita, heterodera glycines, and radopholus similis. three concentrations of tannic acid, 0.1, 1.0, and 10 g/500 cm(3) of soil, were applied preplant (powder) and at-plant (powder and drench) into soil infested with m. arenaria. tannic acid at the ...199719274278
resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in wild accessions of gossypium hirsutum and g. barbadense from mexico.forty-six accessions of g. hirsutum and two of g. barbadense were examined for resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in environmental growth chamber experiments, with the objective of finding new sources of resistance. only the g. barbadense accessions, tx-1347 and tx-1348, supported significantly less reproduction by r. reniformis than the susceptible control, deltapine 16 (usda accession sa-1186). however, they were highly susceptible to m. incognita race 3. t ...199719274280
inoculum densities of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita in relation to the development of fusarium wilt and the phenology of cotton plants (gossypium hirsutum).abstract development of fusarium wilt in upland cotton (gossypium hirsutum) usually requires infections of plants by both meloidogyne incognita and fusarium oxysporum f. sp. vasinfectum. in this study, the soil densities of m. incognita and f. oxysporum f. sp. vasinfectum and the incidence of fusarium wilt in three field sites were determined in 1982-1984. multiple regression analysis of percent incidence of fusarium wilt symptoms on population densities of m. incognita and f. oxysporum f. sp. v ...199718945178
replacement series: a tool for characterizing competition between phytoparasitic nematodes.the replacement series approach was used to detect and define competition between meloidogyne incognita (mi) and rotylenchulus reniformis (rr) on soybean. in three greenhouse tests, soybean cv. davis seedlings were inoculated with 1,000 vermiform nematodes in the following mi:rr ratios: 0:0, 100:0, 75:25, 50:50, 25:75, and 0:100. after 86 days, relative nematode-yield values (number of each species in mixed culture divided by number in nonmixed culture) were calculated based on nematodes in soil ...199719274132
antioxidant enzymes in phytoparasitic nematodes.presence of different antioxidant enzymes, such as superoxide dismutase (sod), catalase, and ascorbate, p-phenilendiamine-pyrocathecol (ppd-pc), o-dianisidine, and guaiacol isoperoxidases, was shown in the phytoparasific nematode species meloidogyne incognita, m. hapla, globodera rostochiensis, g. pallida, heterodera schachtii, h. carotae, and xiphinema index. the activity of the enzymes tested differed among the life stages examined. sod was present in cysts but was not detected in meloidogyne ...199719274144
extraction of root-associated meloidogyne incognita and rotylenchulus reniformis.a technique based on physical maceration of root tissue was developed to extract vermiform and swollen stages of meloidogyne incognita and rotylenchulus reniformis. experiments conducted on soybean and tomato evaluated the efficiency of method (stir, grind), naoc1 concentration (0%, 0.5%), and duration (lx, 2x) on extraction of nematodes and eggs from 60-day-old populations. root-associated populations of r. reniformis were considerably lower than those of m. incognita, so development of the met ...199719274151
antibodies from chicken eggs as probes for antigens from pasteuria penetrans endospores.the bacteria pasteuria spp. have been identified as among the most promising of several microbial organisms currently under investigation as biological control agents of plant-parasitic nematodes. as part of our goal to develop methods to discriminate isolates of pasteuria penetrans with different host preferences, we investigated the potential of developing antibody probes to identify endospores of different isolates of p. penetrans. polyclonal igy antibodies were raised in chickens against end ...199719274158
unique immunogenic proteins in heterodera glycines eggshells.polyclonal antibodies were raised against heterodera glycines eggshells to determine the feasibility of developing an immunoassay for h. glycines eggs. an indirect enzyme-linked immunosorbent assay (elisa) was developed from anfisera collected 10 weeks after the initial injection. from serial dilutions of sonicated eggshells or whole eggs, a sensitivity of detection to 5 ng/ml sonicated eggshells or 1 egg of h. glycines was determined. the method of eggshell preparation had no effect on the anti ...199719274159
tolerance to rotylenchulus reniformis and resistance to meloidogyne incognita race 3 in high-yielding breeding lines of upland cotton.field experiments in 1992 and 1994 were conducted to determine the effect of rotylenchulus reniformis, reniform nematode, on lint yield and fiber quality of 10 experimental breeding lines of cotton (gossypium hirsutum) in untreated plots or plots fumigated with 1,3-dichloropropene. controls were la. rn 1032, a germplasm line possessing some resistance to r. reniformis, and stoneville 453, a cultivar that is susceptible to reniform nematode. several breeding lines produced greater lint yields tha ...199719274165
differential response to root-knot nematodes in prunus species and correlative genetic implications.responses of 17 prunus rootstocks or accessions (11 from the subgenus amygdalus and 6 from the subgenus prunophora) were evaluated against 11 isolates of meloidogyne spp. including one m. arenaria, four m. incognita, four m. javanica, one m. hispanica, and an unclassified population from florida. characterization of plant response to root-knot nematodes was based on a gall index rating. numbers of females and juveniles plus eggs in the roots were determined for 10 of the rootstocks evaluated aga ...199719274170
relationship between meloidogyne incognita and rotylenchulus reniformis as influenced by soybean genotype.the effect of soybean genotype on competition between meloidogyne incognita race 2 (mi) and rotylenchulus reniformis (rr) was evaluated in greenhouse and microplot replacement series experiments. soil in pots containing seedlings of 'davis' (susceptible to mi) or 'buckshot 66' (resistant to mi) was infested with 1,000 vermiform individuals in the following mi:rr ratios: 0:0, 100:0, 75:25, 50:50, 25:75, or 0:100. after 91 days, the relative nematode yields (number of nematodes in mixed culture di ...199719274173
effects of monoclonal antibodies, cationized ferritin, and other organic molecules on adhesion of pasteuria penetrans endospores to meloidogyne incognita.the incidence of adhesion of pasteuria penetrans endospores to meloidogyne incognita second-stage juveniles (j2) was studied after pretreatment of the latter with monoclonal antibodies (mab), cationized ferritin, and other organic molecules in replicated trials. monoclonal antibodies developed to a cuticular epitope of m. incognita second-stage juveniles gave significant reductions in attachment of p. penetrans endospores to treated nematodes. mab bound to the entire length of j2 except for the ...199719274193
effects of temperature on resistance in phaseolus vulgaris genotypes and on development of meloidogyne species.phaseolus vulgaris lines with heat-stable resistance to meloidogyne spp. may be needed to manage root-knot nematodes in tropical regions. resistance expression before and during the process of nematode penetration and development in resistant genotypes were studied at pre- and postinoculation temperatures of 24 degrees c and 24 degrees c, 24 degrees c and 28 degrees c, 28 degrees c and 24 degrees c, and 28 degrees c and 28 degrees c. resistance was effective at all temperature regimes examined, ...199719274137
continual green-fluorescent protein monitoring of cauliflower mosaic virus 35s promoter activity in nematode-induced feeding cells in arabidopsis thaliana.the responsiveness of the cauliflower mosaic virus 35s promoter in feeding sites developed by both sexes of heterodera schachtii and female meloidogyne incognita has been studied. the objective was to establish the value of green-fluorescent protein (gfp) as a nondestructive reporter gene system for characterizing promoter activity at nematode feeding sites in vivo. growth units were devised that allowed individual feeding sites in roots of arabidopsis thaliana to be observed by both bright-fiel ...19979100383
resistance to both cyst and root-knot nematodes conferred by transgenic arabidopsis expressing a modified plant cystatin.plant nematodes are major pests of agriculture. transgenic plant technology has been developed based on the use of proteinase inhibitors as nematode anti-feedants. the approach offers prospects for novel plant resistance and reduced use of environmentally damaging nematicides. a modified rice cystatin, oc-i delta d86, expressed as a transgene in arabidopsis thaliana, has a profound effect on the size and fecundity of females for both heterodera schachtii (beet-cyst nematode) and meloidogyne inco ...19979301094
effects of meloidogyne spp. and rhizoctonia solani on the growth of grapevine rootings.a disease complex involving meloidogyne incognita and rhizoctonia solani was associated with stunting of grapevines in a field nursery. nematode reproduction was occurring on both susceptible and resistant cultivars, and pot experiments were conducted to determine the virulence of this m. incognita population, and of m. javanica and m. hapla populations, to v. vinifera cv. colombard (susceptible) and to v. champinii cv. ramsey (regarded locally as highly resistant). the virulence of r. solani is ...199719274149
image analysis of the growth of globodera pallida and meloidogyne incognita on transgenic tomato roots expressing cystatins.an approach based on image analysis that enables rapid collection and analysis of nematode size and shape during growth is reported. this technique has been applied to assess meloidogyne incognita and globodera pallida during their development over 35 and 42 days, respectively, on transgenic tomato roots expressing the wild-type rice cystatin oc-i or an engineered variant, oc-iad86. morphometric values were established that subdivided enlarged saccate females from other life stages. analysis of ...199619277136
responses of meloidogyne arenaria and m. incognita to green manures and supplemental urea in glasshouse culture.the recent loss of many effective nematicides has led to renewed interest in alternative methods of nematode management. greenhouse experiments were conducted to determine the effects of rapeseed and velvetbean green manures, and supplemental urea, on the root-knot nematodes meloidogyne arenaria and m. incognita. green manures were incorporated with m. arenaria-infested soil using rates totaling 200,300, and 400 mg n/kg soil. squash plants grown in this soil were evaluated using a gall index and ...199619277190
role of nematodes, nematicides, and crop rotation on the productivity and quality of potato, sweet potato, peanut, and grain sorghum.the objective of this experiment was to determine the effects of fenamiphos 15g and short-cycle potato (po)-sweet potato (sp) grown continuously and in rotation with peanut (pe)-grain sorghum (gs) on yield, crop quality, and mixed nematode population densities of meloidogyne arenaria, m. hapla, m. incognita, and mesocriconema ornatum. greater root-gall indices and damage by m. hapla and m. incognita occurred on potato than other crops. most crop yields were higher and root-gall indices lower fro ...199619277157
surface coat of meloidogyne incognita.the nematode surface coat is defined as an extracuticular component on the outermost layer of the nematode body wall, visualized only by electron microscopy. surface coat proteins of meloidogyne incognita race 3 infective juveniles were characterized by electrophoresis and western blotting of extracts from radioiodine and biotin-labeled nematodes. extraction of labeled nematodes with cetyltrimethylammonium bromide yielded a principal protein band larger than 250 kda and, with water soluble bioti ...199619277137
meloidogyne incognita infested soil amended with chicken litter.the effects of chicken litter on meloidogyne incognita in cotton, gossypium hirsutum cv. dpl50 were determined in field microplots. litters (manure and pine-shaving bedding) from a research facility and a commercial broiler house were used. treatments consisted of 0.25%, 0.5%, and 1% litter by dry weight of soil for each kind of litter. three control treatments consisted of soil not amended with litter, with and without nematodes, and one treatment to which mineral fertilizer was added at a nitr ...199619277155
impact of soil texture on the reproductive and damage potentials of rotylenchulus reniformis and meloidogyne incognita on cotton.the effects of soil type and initial inoculum density (pi) on the reproductive and damage potentials of meloidogyne incognita and rotylenchulus reniformis on cotton were evaluated in microplot experiments from 1991 to 1993. the equilibrium nematode population density for r. reniformis on cotton was much greater than that of m. incognita, indicating that cotton is a better host for r. reniformis than m. incognita. reproduction of m. incognita was greater in coarse-textured soils than in fine-text ...199619277171
greenhouse evaluation of selected soybean germplasm for resistance to north carolina populations of heterodera glycines, rotylenchulus reniformis, and meloidogyne species.selected soybean genotypes were evaluated for resistance to north carolina populations of the soybean cyst nematode heterodera glycines, the root-knot nematodes meloidogyne incognita races 3 and 4, m. arenaria races 1 and 2, m. javanica, and the reniform nematode rotylenchulus reniformis in two greenhouse tests. populations of cyst nematode used in the first test were cultures from field samples originally classified as races 1-5, and those used in the second test included inbred cyst lines that ...199619277179
damage potential and reproduction of meloidogyne incognita race 3 and m. arenaria race 1 on kenaf.the effects of meloidogyne incognita race 3 and m. arenaria race 1 on growth of kenaf cv. everglades 41 was determined under greenhouse conditions. seedlings of kenaf were inoculated with initial population densities (pi) of 0, 625, 1,250, 2,500, 5,000, and 10,000 eggs/plant and placed on greenhouse benches in a randomized complete block design. plant growth and nematode reproduction were assessed 6 and 12 weeks after inoculation. growth suppression of kenaf in response to increasing pi was obse ...199619277193
secretory granule proteins from the subventral esophageal glands of the potato cyst nematode identified by monoclonal antibodies to a protein fraction from second-stage juveniles.sodium dodecyl sulfate-extracted proteins from second-stage juveniles (j2) of the potato cyst nematode globodera rostochiensis were fractionated by preparative continuous flow electrophoresis, and monoclonal antibodies (mabs) were raised against the 38- to 40.5-kda protein fraction. screening of the hybridoma culture fluids by immunofluorescence microscopy of j2 resulted in the identification of 12 mabs that bound specifically to the subventral esophageal glands. on western blots of j2 these mab ...19968589421
expression and functional characterization of a single chain fv antibody directed against secretions involved in plant nematode infection process.expression in plants of antibodies directed against proteins essential for pathogenesis could provide an alternative approach to engineer new resistance traits into crops. salivary secretions of the root-knot nematode meloidogyne incognita are known to play a key role during this nematode infection process. from a hybridoma cell line producing an igm monoclonal antibody specific to these secretions, we have constructed a synthetic gene that encodes an antigen-binding single-chain fv protein (scf ...19968645292
identification and in situ and in vitro characterization of secreted proteins produced by plant-parasitic nematodes.secretions of plant-parasitic nematodes which are released into plant tissue may play critical roles in plant-nematode interactions. the identification and characterization of these molecules are of fundamental importance and may help to facilitate the development of novel strategies to interfere with nematode infection of plants and thereby decrease nematode-induced damage to crops. an antibody-based approach was used to isolate molecules present on the nematode surface and in nematode secretio ...19968939056
suppression of meloidogyne incognita and m. javanica by pasteuria penetrans in field soil.the role of pasteuria penetrans in suppressing numbers of root-knot nematodes was investigated in a 7-year monocuhure of tobacco in a field naturally infested with a mixed population of meloidogyne incognita race 1 and m. javanica. the suppressiveness of the soil was tested using four treatments: autoclaving (ac), microwaving (mw), air drying (dr), and untreated. the treated soil bioassays consisted of tobacco cv. northrup king 326 (resistant to m. incognita but susceptible to m. javanica) and c ...199619277344
identification and characterization of the meloidogyne incognita col1 cuticle collagen gene. 19969010847
effect of yard waste compost on nematode densities and maize yield.the effects of a yard waste compost on densities of plant-parasitic nematodes and forage yield of maize (zea mays) were determined over three seasons in two sites in north florida. in each test, the experimental design was a randomized complete block with five replications and three treatments: 269 mt/ha of a yard waste compost c:n ratio = 35:1 to 46:1) applied to the soil surface as a mulch, 269 mt/ha of compost incorporated into the soil, and an unamended control. of the nematodes found in the ...199619277191
screening of carnation cultivars for resistance to meloidogyne incognita.a total of 33 carnation cultivars cultured in korea were screened for resistance to the southern root-knot nematode, meloidogyne incognita. carnations were tested by either inoculating with 5,000 eggs or by transplanting into a mixture of bedding medium and soil infested with an average of 435 second-stage juveniles/300 cm(3) soil. cultivars, desio, castelaro, kappa, rara, izu pink, target, and antalia were highly resistant to m. incognita. twelve cultivars were moderately resistant, and the rem ...199619277188
mentha x piperita, mentha spicata and effects of their essential oils on meloidogyne in soil.six peppermint (mentha x piperita) and six spearmint (m. spicata) pi accessions were inoculated with meloidogyne incognita race 3 and m. arenaria race 2, under greenhouse conditions. no galls formed on roots of any of the plants inoculated with 1,800 eggs/pot. fewer than two galls per root system formed on three pi accessions of peppermint inoculated with m. incognita at 5,400 eggs/pot. only one peppermint accession developed galls when inoculated with m. arenaria, whereas none of the spearmint ...199619277186
early root response to meloidogyne incognita in resistant and susceptible alfalfa cultivars.the early events of meloidogyne incognita behavior and associated host responses following root penetration were studied in resistant (cv. moapa 69) and susceptible (cv. lahontan) alfalfa. ten-day-old seedlings of alfalfa cultivars were inoculated with second-stage juveniles (j2) and harvested 12, 24, 48, and 72 hours and 7, 14, and 21 days later. both cultivars supported similar root penetration and initial j2 migration. by 72 hours after inoculation the majority of j2 were amassed inside the v ...199619277165
differentiation of meloidogyne incognita and m. arenaria novel resistance phenotypes in lycopersicon peruvianum and derived bridge-lines.lycopersicon peruvianum pi 270435 clone 2r2 and pi 126443 clone 1mh were crossed reciprocally with three l. esculentum-l. peruvianum bridge-lines. the incongruity barrier between the two plant species was overcome; f1 progeny were obtained from crosses between four parental combinations without embryo-rescue culture. hybridity was confirmed by leaf and flower morphology and by the production of nematode-resistant f1 progeny on homozygous susceptible parents. clones of the five f1 bridgeline hybr ...199624162431
relationships between meloidogyne incognita resistance genes in lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence.resistance to meloidogyne incognita (kofoid and white) chitwood in clones of lycopersicon peruvianum (l.) mill. pi 126443-1mh, 270435-2r2 and 2704353mh, their f1, a field-produced f2, and their test-cross (tc1) populations, was evaluated based on egg masses and eggs produced on root systems. reactions to m. incognita isolates differing in virulence to gene mi were determined at 25°c (mi expressed) and 32°c (mi not expressed). pi 126443-1mh, 270435-2r2, 270435-3mh, and their f1 progenies were res ...199624162430
effect of simulated rainfall on efficacy and leaching of two formulations of fenamiphos.recoverable fenamiphos in the soil and residue in squash following different simulated rainfall treatments after nematicide application were determined in a 2-year study. efficacy of fenamiphos also was evaluated. fenamiphos treatments (3 sc and 15 g) were broadcast (6.7 kg a.i./ha) over plots and incorporated into the top 15 cm of soil immediately before planting 'dixie hybrid' squash. simulated rainfall treatments of 0, 2.5, and 5.0 cm water were applied 1 day after fenamiphos application. soi ...199619277156
comparison of two sympatric pasteuria populations isolated from a tropical vertisol soil.an isolate of pasteuria (designated ppmj) recovered from the root-knot nematode meloidogyne javanica, was characterized using host preference, spore morphometrics, and serology, and compared with another sympatric pasteuria isolate (designated pphc) collected from the cyst nematode, heterodera cajani. ppmj spores were larger (x 1.5) than the pphc spores and had a mean diameter of 3.4 μm after fixation for electron microscopy. the central body of ppmj spores was about twice as big as the central ...199624415311
repulsion of meloidogyne incognita by alginate pellets containing hyphae of monacrosporium cionopagum, m. ellipsosporum, or hirsutella rhossiliensis.the responses of second-stage juveniles (j2) of meloidogyne incognita race 3 to calcium alginate pellets containing hyphae of the nematophagous fungi monacrosporiura cionopagum, m. ellipsosporum, and hirsutella rhossiliensis were examined using cylinders (38-mm-diam., 40 or 72 mm long) of sand (94% <250-mum particle size). sand was wetted with a synthetic soil solution (10% moisture, 0.06 bar water potential). a layer of 10 or 20 pellets was placed 4 or 20 mm from one end of the cylinder. after ...199619277129
a molecular study of root-knot nematode-induced feeding sites.in a compatible interaction, root-knot nematodes (meloidogyne) induce a sophisticated feeding site shortly after they have penetrated the plant root. the feeding site contains metabolically highly active giant cells. to gain insight into the molecular aspects that are typical for giant cells, a cdna library from tomato roots infected with meloidogyne incognita was differentially screened to find induced genes. among the genes identified, two extensin genes (lemmi8 and lemmi11) and a lea-like gen ...19968580972
effects of irrigation, nitrogen, and a nematicide on pearl millet.pearl millet is used mainly as a temporary forage crop in the southern united states. a new pearl millet hybrid has potential as a major grain crop in the united states. the effects of nematodes, irrigation, a nematicide, and nitrogen rates on a new pearl millet grain hybrid, hgm-100, and nematode population changes were determined in a 2-year study. root-knot nematodes (meloidogyne incognita race 1) entered the roots of pearl millet and caused minimal galling, but produced large numbers of eggs ...199519277324
effects and carry-over benefits of nematicides in soil planted to a sweet corn-squash-vetch cropping system.the effects of irrigation on the efficacy of nematicides on meloidogyne incognita race 1 population densities, yield of sweet corn, and the carry-over of nematicidal effect in the squash crop were determined in a sweet corn-squash-vetch cropping system for 3 years. fenamiphos 15g and aldicarb 15g were applied at 6.7 kg a.i./ha and incorporated 15 cm deep with a tractor-mounted rototiller. ethylene dibromide (edb) was injected at 18 kg a.i./ha on each side of the sweet corn rows (total 36 kg a.i. ...199519277323
effect of simulated rainfall on leaching and efficacy of fenamiphos.there is increasing concern in the united states about the pesticide movement in soil, groundwater contamination, and pesticide residue in food. the objective of this study was to determine the efficacy, degradation, and movement of fenamiphos (nemacur 15g) in the soil and residues in squash fruit as influenced by four simulated rainfall treatments (2.5 or 5.0 cm each applied 1 or 3 days after nematicide application) under field conditions. in 1990, concentrations of fenamiphos were greater in t ...199519277322
effect of yard waste compost on plant-parasitic nematode densities in vegetable crops.the effects of yard-waste compost on densities of plant-parasitic nematodes were determined on four crops at two sites in north florida. separate experiments were conducted with sweet corn (zea mays), cowpea (vigna unguiculata), yellow squash (cucurbita pepo), and okra (hibiscus esculentus). in each test, the design was a randomized complete block replicated four times and involving three treatments: 269 mt/ha yard-waste compost applied to the soil surface as a mulch, 269 mt/ha compost incorpora ...199519277320
influence of meloidogyne incognita on the water relations of cotton grown in microplots.the effects of meloidogyne incognita on the growth and water relations of cotton were evaluated in a 2-year field study. microplots containing methyl bromide-fumigated fine sandy loam soil were infested with the nematode and planted to cotton (gossypium hirsutum l.). treatments included addition of nematodes alone, addition of nematodes plus the insecticide-nematicide aldicarb (1.7 kg/ha), and an untreated control. meloidogyne incognita population densities reached high levels in both treatments ...199519277313
effect of ammonium ions on egg hatching and second-stage juveniles of meloidogyne incognita in axenic tomato root culture.eggs, either dispersed or in masses, and second-stage juveniles (j2) of meloidogyne incognita were exposed to different concentrations of ammonium ions in a nutrient agar medium upon which excised tomato roots were growing. egg hatch and j2 penetration of the roots was slowed or inhibited at high (54 and 324 mg/liter) but not at low (1.5 and 9 mg/liter) concentrations of ammonium nitrate. the effect of ammonium on j2 appeared to be temporary and reversible. high potassium nitrate concentration ( ...199519277298
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