Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| mean retention time in digestive tract and digestion of fresh perennial ryegrass by lactating dairy cows: influence of grass maturity and comparison with a maize silage diet. | the effects of grass maturity on the mean retention time of liquid and particles, digestibility and duodenal nutrient flows were measured by feeding second cuts of perennial ryegrass to 3 fistulated lactating dairy cows over 2 successive periods (late june and mid-july, 28 and 49 d regrowth, respectively). fresh forage was given ad libitum with 12% (dry matter (dm) basis) concentrate. the increase in maturity lowered grass nitrogen content, organic matter (om) digestibility and non-ammoniacal ni ... | 1994 | 8129846 |
| comparison of skin-prick test assay and reverse enzyme immunoassay competition (reina-c) for biological activity of allergens. | an in vitro technique for measuring the relative potency of allergenic extracts has been developed and compared with the dose-response skin-prick test. the in vitro technique is a modification of an elisa based on the method of antigen capture using polystyrene microtitre wells as solid phase (reina-competition) and is also based on a dose-response. both methods have been applied to allergen extracts of dermatophagoides pteronyssinus, phleum pratense, secale cereale, lolium perenne, plantago lan ... | 1994 | 8187028 |
| prevalence of patent and latent atopy among a general normal adult population in the south east of france by rast investigation and correlation with circulating total ige levels. | a population of 512 adults (men: 266, 52%, women: 246, 48%) with mean age 41.4 years (s.d. 10.7) was investigated. this population was subdivided into five groups: marseilles city, manosque a small town in a prealpine area, the area surrounding the etang de berre, a rural area in northern bouches du rhône and chateaurenard, a small town in an intensive agricultural area. rasts to a panel of eight allergens (lolium perenne, parietaria officinalis, cupressus sempervirens, olea europea, cat dander, ... | 1993 | 8160568 |
| nucleotide sequence of cdna encoding the group ii allergen of cocksfoot/orchard grass (dactylis glomerata), dac g ii. | cocksfoot/orchard grass (dactylis glomerata) anther cdna clones encoding the group ii allergen dac g ii were previously isolated on the basis of immunoreactivity of human, rabbit, and murine antibodies with a 24-kda protein expressed as a fusion protein with beta-galactosidase. nucleotide sequencing reveals an open reading frame predicting expression of a 98-amino-acid (11-kda) polypeptide exhibiting > 90% homology with the group ii allergen of lolium perenne, lol p ii. in vitro translation of d ... | 1993 | 8116860 |
| effects of partial replacement of ryegrass by low protein feeds on rumen fermentation and nitrogen loss by dairy cows. | three rumen fermentation studies in combination with three feeding trials were carried out to investigate the effect of partial replacement of heavily fertilized perennial ryegrass by low protein feedstuffs on ph and concentrations of vfa and nh3 n in the rumen and on n excretion in milk, urine, and feces by dairy cows. feedstuffs tested were high fiber concentrate mixtures based on sugar beet pulp and soybean hulls and high starch concentrate mixtures based on corn (experiments 1 and 2), corn s ... | 1993 | 8227624 |
| in situ dry matter, protein, and fiber degradation of perennial forages. | eight forages (alfalfa, birdsfoot trefoil, red clover, bromegrass, orchardgrass, perennial ryegrass, quackgrass, and timothy) at three maturities were evaluated for ruminal dm, cp, and ndf degradation kinetics. duplicate dacron bags were incubated for 0, 3, 6, 10, 13, 25, 48, and 72 h in two late lactation holstein cows fitted with ruminal cannulas over eight experimental periods. species and maturity effects were observed for soluble, slow, and undegraded fractions; degradation rate; and rumina ... | 1993 | 8227665 |
| molecular characterization of phl p ii, a major timothy grass (phleum pratense) pollen allergen. | grass pollen allergens belong to the most important and widespread elicitors of pollen allergy. using serum ige from a grass pollen allergic patient, a complete cdna encoding a group ii allergen was isolated from a timothy grass (phleum pratense) pollen expression library. the deduced amino acid sequence of the phl p ii allergen shows an average sequence identity of 61% with the protein sequences determined for group ii/iii allergens from rye grass (lolium perenne) and a sequence identity of 43% ... | 1993 | 8262175 |
| effect of mixing allergenic extracts containing helminthosporium, d. farinae, and cockroach with perennial ryegrass. | allergenic extracts used for immunotherapy often contain mixes of different allergens. studies have shown that certain allergenic extracts contain enzymes that can cause a decrease in grass pollen allergenicity when mixed with grass extracts. glycerin and buffered saline with phenol (bsp) extracts containing helminthosporium interseminatum, d. farinae, and cockroach were mixed with perennial ryegrass extracts and analyzed 7, 30, 60, and 90 days after mixing to determine the mixing effect of thes ... | 1993 | 8372997 |
| changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defaunated sheep. | changes in the microbial populations, their activities, and the ruminal fermentation were monitored for 50 d following the reintroduction of ciliate protozoa into four defaunated sheep. a protozoal population was reestablished successfully in each recipient, using a washed inoculum containing approximately 10(3) cells, although there were between-animal differences in the rates of recolonization and genus establishment. entodinium spp. predominated in the initial stages of the refaunation period ... | 1993 | 8439876 |
| ryegrass staggers in heifers: a new mycotoxicosis in argentina. | aberdeen angus (aa) and crossbred aa x hereford 8-9 mo old heifers (560 animals) showed a typical perennial ryegrass (prg) staggers syndrome after grazing 26 d in a 25-hectare paddock of pure lolium perenne. effects were observed in about 50% of the herd. when forced to move, the animals showed incoordination, weakening of the forelimbs, falling on the chest and neck, and moving in this position during the first steps before recovering a normal gait. in severely affected animals all of the 4 lim ... | 1993 | 8470358 |
| lol p i-induced il-4 and ifn-gamma production by peripheral blood mononuclear cells of atopic and nonatopic subjects during and out of the pollen season. | the reciprocal effects of il-4 and ifn-gamma on ige synthesis have been well established. it has also been shown that these two lymphokines are secreted by different subsets of cd4+ t cells (th1 and th2), and that th2 helper t lymphocytes could be involved in the pathophysiology of allergic diseases. but little is known about the effects of an allergen on the profile of lymphokine synthesis by human peripheral blood mononuclear cells (pbmcs) of allergic and nonallergic subjects. we studied the p ... | 1993 | 8473684 |
| relationships among non-acremonium sp. fungal endophytes in five grass species. | many cool-season grasses (subfamily pooideae) possess maternally transmitted fungal symbionts which cause no known pathology and often enhance the ecological fitness and biochemical capabilities of the grass hosts. the most commonly described endophytes are the acremonium section albo-lanosa spp. (acremonium endophytes), which are conidial anamorphs (strictly asexual forms) of epichloë typhina. other endophytes which have been noted are a gliocladium-like fungus in perennial ryegrass (lolium per ... | 1993 | 8517749 |
| in vitro synthesis of il-4 by human cd4+ t cells requires repeated antigenic stimulation. | although th2 helper cell clones produce il-4 and il-5, cd4+ t cells taken fresh from lymphoid organs of mice produce il-2 and some ifn-gamma, but not il-4 or il-5. the exact parameters that enhance the synthesis of il-4, il-5, and particularly il-10 from resting antigen-specific cd4+ t cells is not yet clear. we therefore examined the kinetics of, and the parameters that affect, the development of il-4, il-5, and il-10 production in bulk populations of antigen-specific human cd4+ t cells. we dem ... | 1993 | 7902215 |
| a clearing technique for detecting the fungal endophyte acremonium sp. in grasses. | leaf tissue of tall fescue festuca arundinacea schreb., hard fescue festuca ovina l., red fescue festuca rubra l. and perennial ryegrass lolium perenne l. was stained with rose bengal or aniline blue to detect the presence of the fungal endophyte acremonium sp.. specimens were cleared using methyl salicylate, an optical clearing agent, and viewed using bright field microscopy. tissue was preserved as dried tissue or stored in 70% aqueous ethyl alcohol before staining and clearing. tissue was obs ... | 1993 | 7684264 |
| biological activity of monoclonal anti-idiotypic antibody representing the internal image of the major allergenic component of lolium perenne pollen. | upon immunization with an anti-lol p i (major allergenic component of lolium perenne pollen) monoclonal antibody, we have previously produced anti-idiotypic monoclonal antibody (a7h2) displaying some internal image properties. the present study was designated to evaluate the capacity of this anti-idiotypic monoclonal antibody to mimic functionally the antigen by triggering histamine release from basophils of patients allergic to lol p i. anti-idiotypic monoclonal antibody, as the antigen, could ... | 1993 | 7691293 |
| cloning, expression, and immunological characterization of recombinant lolium perenne allergen lol p ii. | the molecular cloning of the cdna encoding for an isoallergenic form of lol p ii, a major rye grass (lolium perenne) pollen allergen, was performed by polymerase chain reaction amplification on mrna extracted from pollen. the amino acid sequence derived from the cdna was truncated by 4 and 5 residues at the nh2- and cooh-terminal ends, respectively, and differed only in one position from that previously reported. this cdna was expressed in escherichia coli by fusion to the carboxyl terminus of t ... | 1993 | 7691817 |
| cdna cloning of a major allergen from timothy grass (phleum pratense) pollen; characterization of the recombinant phl pv allergen. | we isolated a cdna encoding a major grass pollen allergen from a timothy grass (phleum pratense) pollen expression cdna library using allergic patients' ige. the complete cdna encoded an allergen that binds ige from about 80% of grass pollen-allergic patients. significant sequence homology was found to other major grass pollen allergens from kentucky bluegrass (poa pratense) as well as from rye grass (lolium perenne) which originally were believed to form different identities. using different mo ... | 1993 | 7691956 |
| a novel fungal protease expressed in endophytic infection of poa species. | the fungus acremonium typhinum produces a novel endoprotease during symbiotic endophytic infection of the grass, poa ampla. this protease is unusual because it is highly active in the presence of sodium dodecyl sulfate. the enzyme is a thiol-containing serine protease and is localized to a crude membrane fraction. similar protease activity has been detected in endophyte-infected poa autumnalis and poa sylvestris plants. expression of this protease may be important in endophytic infection of poa ... | 1993 | 12231854 |
| confirmation of the airborne occurrence of micron-size airborne pollen antigen carrying particles by immunoblotting. | we confirmed the existence of micron-size airborne particles carrying major pollen antigen of cryptomeria japonica (cry j l) and lolium perenne (lol p l) by means of an immunoblotting technique and examination by light microscopy. the size of the spots on a nitrocellulose membrane, marking the presence of antigen on the sampler's tape, varied considerably under light microscopic examination. spots smaller than pollen grains and micron-size spots were observed together with large spots which seem ... | 1993 | 8489692 |
| variability of crossreactivity of ige antibodies to group i and v allergens in eight grass pollen species. | crossreactivity to dactylis glomerata, festuca rubra, phleum pratense, anthoxanthum odoratum, secale cereale, zea mays, and phragmites communis of ige antibodies against lol p i or lol p v was investigated by means of rast-inhibition. within a group of sera the degree of crossreactivity was demonstrated to be highly variable. individual sera were not always equally crossreactive to all pollen species. a high degree of crossreactivity for group i allergens did not necessarily implicate the same f ... | 1992 | 1382820 |
| the effect of nitrate-nitrogen supply on bacteria and bacterial-feeding fauna in the rhizosphere of different grass species. | microbial growth in the rhizosphere is affected by the release of organic material from roots, so differences in carbon budgets between plants may affect their rhizosphere biology. this was tested by sampling populations of bacteria and bacteriophagous fauna from the rhizosphere of lolium perenne, festuca arundinacea, poa annua, and poa pratensis, under conditions of high and low nitrate availability. concentrations of soluble phenolics and lignin varied considerably between the species but were ... | 1992 | 28313466 |
| protein digestion and intestinal amino acids in dairy cows fed fresh lolium perenne with different nitrogen contents. | an experiment was carried out to study digestion and intestinal aa in three ruminally and duodenally cannulated, lactating cows fed freshly cut grass (lolium perenne) fertilized with 500 and 275 kg of n/ha per yr, respectively. high n grass was fed in june and october, and low n grass was offered in july and september. composite samples of the grass fed in each period also were tested for in situ degradation of om, cp, and ndf. when low n grass was fed, the digestibilities of om and cp were lowe ... | 1992 | 1401372 |
| il-4 and ifn (alpha and gamma) exert opposite regulatory effects on the development of cytolytic potential by th1 or th2 human t cell clones. | the cytolytic potential of a total number of 118 cd4+ human t cell clones specific for purified protein derivative (ppd) from mycobacterium tuberculosis, tetanus toxoid, lolium perenne group i allergen (lol p i), poa pratensis group ix allergen (poa p ix), or toxocara canis excretory/secretory antigen(s) (tes) was assessed by both a lectin (pha)-dependent and a mhc-restricted lytic assay and compared with their profile of cytokine secretion. the majority of clones with th1 or th0 cytokine profil ... | 1992 | 1401925 |
| perennial ryegrass staggers in fallow deer (dama dama). | 1992 | 1445073 | |
| the intake, digestion and protein degradation of grazed herbage by early-weaned lambs. | sixty-four intact lambs and twenty-four lambs fitted with a duodenal cannula were weaned at 6 weeks of age and grazed pure species swards of either lucerne (medicago sativa), white clover ((trifolium repens), ryegrass (lolium perenne) or prairie grass (bromus catharticus) for 6 weeks. intake and duodenal digesta flow were estimated when lambs were 8 and 12 weeks of age. lambs grazing the two legume species grew at a similar rate, as did lambs grazing the two grass species. legumes promoted a 38% ... | 1992 | 1445817 |
| recombinant pollen allergens from dactylis glomerata: preliminary evidence that human ige cross-reactivity between dac g ii and lol p i/ii is increased following grass pollen immunotherapy. | we previously described the isolation of three identical complementary dna (cdna) clones, constructed from orchard/cocksfoot grass (dactylis glomerata) anther messenger rna (mrna), expressing a 140,000 mw beta-galactosidase fusion protein recognized by ige antibodies in atopic sera. partial nucleotide sequencing and inferred amino acid sequence showed greater than 90% homology with the group ii allergen from lolium perenne (lol ii) indicating they encode the group ii equivalent, dac g ii. wester ... | 1992 | 1526648 |
| olive (olea europea) and privet (ligustrum vulgare) pollen allergens. identification and cross-reactivity with grass pollen proteins. | protein blotting studies showed that three olive pollen components with mol. wts approximately 18-19, 20 and 40 kd can be considered to be major allergens. for privet pollen, the highest recognition frequencies were for allergens of mol. wts approximately 20, approximately 19, approximately 40 and approximately 70 kd. when results with the 62 subjects examined were separated into groups corresponding to their geographical locations, viz. italy, france and australia, subjects sensitized to olive, ... | 1992 | 1528191 |
| human t cell responses to purified pollen allergens of the grass, lolium perenne. analysis of relationship between structural homology and t cell recognition. | the in vitro proliferative response to purified allergens of the grass, lolium perenne pollens was studied using pbmc from individuals allergic to grass pollens and ag-specific t cell lines and t cell clones derived from them. the pbmc from all 10 subjects studied showed a strong response to lol p i and most of them (8 of 10) also responded to lol p iii. although lol p ii induced a moderate response in 4 of 10 individuals, it did not induce any response in others at all the ag concentrations tes ... | 1992 | 1560197 |
| [threshed english ryegrass (lolium perenne) as a cause of ryegrass 'staggers' in stabled heifers]. | 1992 | 1604452 | |
| group v allergens in grass pollens: iv. similarities in amino acid compositions and nh2-terminal sequences of the group v allergens from lolium perenne, poa pratensis and dactylis glomerata. | monoclonal antibodies (ppv4) raised against phleum pratense group v allergen were used for immuno-affinity chromatography of cross-reacting group v allergens from related grass species. fractions enriched in group v allergen were obtained from lolium perenne, poa pratense and dactylis glomerata extracts. the major components in these fractions were found in the mwr range 25-28 kd. ige binding to these components was shown using a pool of grass allergic sera, by sds-page immunoblotting. these fra ... | 1992 | 1611548 |
| studies of method of conserving grass herbage and frequency of feeding in cattle. 1. voluntary feed intake, digestion and rate of passage. | the effect of method of conserving grass herbage and the frequency of feeding on digestion in and passage from the rumen was studied in growing cattle. a single sward of perennial ryegrass (lolium perenne cv. endura) was cut on 1 d and conserved as silage or hay. these forages were offered as the only feed to twelve rumen-cannulated friesian steers (average initial live weight (lw) 128 kg) either at a restricted level of intake (20 g dry matter (dm)/kg lw; expt 1) or ad lib. (expt 2). in expt 1 ... | 1992 | 1622974 |
| populations of criconemella xenoplax on peach interplanted with certain herbaceous plants. | peach trees growing in field microplots infested with criconemella xenoplax were interplanted with centipedegrass, goosegrass, sicklepod, marigold, buckhorn plantain, showy crotalaria, or perennial ryegrass, or left with weed-free soil as the control. buckhorn plantain reduced nematode numbers, but not to levels acceptable for commercial control. marigold, sicklepod, and goosegrass reduced nematode numbers on some sampling dates but not on others. other plants had no effect or appeared to stimul ... | 1992 | 19283046 |
| host status of different bermudagrasses (cynodon spp.) for the sting nematode, belonolaimus longicaudatus. | thirty-seven warm-season bermudagrass (cynodon spp.) accessions, two cool-season grasses (lolium perenne and festuca arundinacea), 'transvala' digitgrass (digitaria decumbens), and sorghum bicolor were evaluated to determine host suitability and susceptibility to the sting nematode, b. longicaudatus, in a 140-day microcell bioassay. all seven of the evaluated commercial cultivars of cynodon were suitable hosts for b. longicaudatus but varied in their tolerance to the nematode. 'midiron,' 'tifdwa ... | 1992 | 19283057 |
| herbivory by canada geese: diet selection and effect on lawns. | flocks of free-ranging canada geese (branta canadensis) often are considered nuisances when they graze on lawns because they litter the sites with fecal material, and their grazing often is perceived to be detrimental to the turf. i tested whether goose grazing had changed the composition of grass species at 20 sites in connecticut where geese were considered nuisances. at these sites kentucky bluegrass (poa pratensis) was less prevalent in areas grazed heavily by geese than in areas of the same ... | 1991 | 27755658 |
| within-population variation in localized and integrated responses of trifolium repens to biotically patchy environments. | genets of trifolium repens (white clover) were collected from three patches of old permanent pasture dominated by agrostis capillaris, holcus lanatus or lolium perenne. plants derived from the genets were grown with plants of one grass species present on one side of each t. repens, and a different grass species on the other side, in all combinations of two of the three grasses. different modules (a node with its associated internode, leaf, and axillary bud) on the same clover plant responded ind ... | 1991 | 28313200 |
| estimation of ige antibodies to the common allergens by reverse enzyme immunoassay. comparison with the radioallergosorbent test. | a reverse enzyme immunoassay (reina) is described, in which polystyrene microtiter wells are sensitized with murine monoclonal anti-human ige, and then sequentially allowed to react with patient's serum, peroxidase-labeled allergens and substrate. the results obtained with the sera of patients allergic to lolium perenne grass pollen, the tree pollens of betula alba and olea europea, the epithelia of cat and dog, the mite dermatophagoides pteronyssinus, or to the foodstuffs cow's milk, chicken eg ... | 1991 | 1669584 |
| increased numbers of th2-like cd4+ t cells in target organs and in the allergen-specific repertoire of allergic patients. possible role of il-4 produced by non-t cells. | phytohemagglutinin (pha)-induced human t cell clones (tcc) derived from conjunctival flogistic tissues of 3 patients with vernal conjunctivitis produced unusually high amounts of interleukin-4 (il-4) and no, or limited amounts of, gamma-interferon (ifn-gamma). allergen (dermatophagoides pteronyssinus or lolium perenne group i)-specific tcc derived from peripheral blood of two atopic donors produced significantly higher amounts of il-4 and significantly lower amounts of ifn-gamma than tcc specifi ... | 1991 | 1682270 |
| the characteristics of antigen define the cellular source and kinetics of histamine-releasing factor induced by human peripheral-blood mononuclear cells. | peripheral-blood leucocytes from dermatophagoides pteronyssinus- and/or lolium perenne-allergic patients produce in vitro histamine-releasing factor (hrf) in an allergen-specific and concentration-dependent relationship. maximum production of hrf occurred in cultures containing as little as 10-100 pg/ml crude allergen extract, although a second peak occurred at higher concentrations (10-100 micrograms/ml). while hrf was detectable in 1-hour cultures, maximal production required 24 h in culture. ... | 1991 | 1718885 |
| nitrogen transactions along the digestive tract of lambs concurrently infected with trichostrongylus colubriformis and ostertagia circumcincta. | twelve lambs, paired on the basis of live weight, were cannulated in the abomasum, in the proximal jejunum approximately 4 m distal to the pylorus and in the terminal ileum. six were infected with 3000 trichostrongylus colubriformis and 3000 ostertagia circumcincta larvae each day for 18 weeks and the remainder were pair-fed to individual infected lambs. all animals were offered ryegrass (lolium perenne)-white clover (trifolium repens) pasture, cut daily. dry matter (dm) intake, live weight, fae ... | 1991 | 1760444 |
| linear dna plasmids of the perennial ryegrass choke pathogen, epichloë typhina (clavicipitaceae). | epichloë typhina is a clavicipitaceous ascomycete which systemically infects grasses, causes choke disease of host inflorescences, and is related to a group of mutualistic grass endophytes. three plasmids of 7.5, 2.1 and 2.0 kilobase pairs were found in mitochondrial dna preparations of an e. typhina isolate from perennial ryegrass (lolium perenne). results of nuclease digestion indicated that the plasmids, designated et7.5l, et2.1l, and et2.0l, were linear, double-stranded dnas with protein lin ... | 1991 | 1782678 |
| allergen- and bacterial antigen-specific t-cell clones established from atopic donors show a different profile of cytokine production. | we have established a large panel of t-cell clones (tccs) specific for dermatophagoides pteronyssinus and lolium perenne group i grass pollen allergens (total, 61) and for tetanus toxoid and protein purified derivative bacterial antigens (total, 38) from the peripheral blood of two atopic individuals and then analyzed their ability to produce interleukin 4 (il-4), il-5, and interferon gamma (ifn-gamma). upon stimulation with phorbol 12-myristate 13-acetate plus anti-cd3 antibody, the great major ... | 1991 | 1827920 |
| effect of ciliate protozoa on the activity of polysaccharide-degrading enzymes and fibre breakdown in the rumen ecosystem. | the effect of ciliate protozoa on the activity of polysaccharide-degrading enzymes in microbial populations from the digesta solids and liquor fractions of rumen contents was examined after the refaunation of ciliate-free sheep with an a-type rumen protozoal population. although the culturable rumen bacterial population was reduced after refaunation the number of fibrolytic micro-organisms detected was higher; the xylanolytic bacterial population and numbers of fungal zoospores were increased af ... | 1991 | 1850400 |
| group v allergens in grass pollens. ii. investigation of group v allergens in pollens from 10 grasses. | in an earlier study an allergen from phleum pratense (timothy) pollen, phl p v, has been isolated and physicochemically characterized. in this study phl p v and immunochemically similar components from other grass pollens (group v allergens) have been investigated using immunoelectrophoretic techniques. to study the allergenic importance of the group v allergens, the allergenic compositions of 10 grass pollen extracts were investigated in crossed radioimmunoelectrophoresis (crie) using 20 sera f ... | 1991 | 1863893 |
| staggers induced by consumption of perennial ryegrass in cattle and sheep from northern california. | staggers was diagnosed in sheep and cattle from the northern california coast. the diagnosis was made on the basis of history of ingestion of perennial ryegrass (lolium perenne) stubble, clinical signs of transient ataxia, which was aggravated by stimulation, and nearly complete recovery after removal of ryegrass as the primary forage. morbidity was high, but death did not occur in any affected animals. the toxic endophyte, acremonium lolii, was in most lower leaf sheaths from the ryegrass. inje ... | 1991 | 1917658 |
| characterization of the major allergen of cynodon dactylon (bermuda grass) pollen, cyn d i. | an allergen from cynodon dactylon (bermuda grass) pollen, cyn d i, has been purified by a combination of concanavalin a-sepharose affinity chromatography, and carboxymethyl-sepharose chromatography. the allergen constitutes the major allergenic component of the pollen extract as observed by immunoelectrophoretic techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an ige-inhibition experiment, and skin testing. in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, cyn d ... | 1991 | 1955635 |
| development of a model to predict forage intake by grazing cattle. | a mathematical model to predict daily dmi and account for effects of energy supplementation on forage intake has been developed in several stages. a previously evaluated dynamic rumen model for sheep was adopted as the fermentation component of the intake model. intake was adjusted to reach a given level of dm fill, which is the sum of the concentrations of each of the dietary fractions within the rumen. differential equations described the rate of change of each nutrient fraction. genetic size ... | 1991 | 2016209 |
| effect of mecoprop drift on some plant species of conservation interest when grown in standardized mixtures in microcosms. | there has been an increasing awareness of potential impacts of herbicide drift on to vegetation in nature reserves and field margin habitats adjacent to treated areas. previous work using single species bioassays has suggested that the effects of a single drift event are confined close to the sprayer (< 10 m). in the present study eight native dicotyledonous species, with and without a perennial grass (lolium perenne), were grown in standardized microcosms in order to study (1) the effects of he ... | 1991 | 15092089 |
| ovine white-liver disease (owld). botanical and chemical composition of pasture grass. | the most important grass species on the ovine white-liver disease (owld) pastures (s) were poa spp., agropyron repens and lolium perenne, while the control pastures (h), where lambs grew well, consisted of poa spp., festuca rubra and agrostis tenuis. the soil was more acidic on the h pastures as compared with the s pastures. owld grass (s grass) contained marginal to deficient amounts of cobalt during the first 2 months of grazing. during 2 years out of 3, the average co content was slightly low ... | 1990 | 2080770 |
| ige-binding proteins from pine (pinus radiata d. don) pollen: evidence for cross-reactivity with ryegrass (lolium perenne). | a complex mixture of pine (pinus radiata d. don) pollen proteins are rapidly released into aqueous solutions. ige-binding proteins have been identified in these extracts using combined sds-page immunoblotting techniques. these ige-binding proteins were detected using atopic patient and commercial pooled human sera known to be high in ryegrass-specific ige. enzyme-immunoassay inhibition studies revealed that leached p. radiata pollen proteins could partially inhibit serum ige binding to ryegrass ... | 1990 | 2086485 |
| two-dimensional gel electrophoretic analyses of kentucky bluegrass and rye grass pollen allergens. detection with a murine monoclonal anti-poa p i antibody and amino terminal amino acid sequence of poa p i allergen. | allergenic extracts of kentucky bluegrass (poa pratensis) and rye grass (lolium perenne) pollen were shown by 2-dimensional (2-d) gel electrophoresis to consist of several hundred protein components. the pollen extracts of the two related grasses had unique 2-d gel patterns. two major grass pollen allergens, poa p i and lol p i, and their isoforms (i.e. isoallergens) were detected and localized on 2-d gels by immunoblotting with anti-poa p i antibody mab 60. poa p i isoallergens were less acidic ... | 1990 | 2101126 |
| the beta-tubulin gene of epichloë typhina from perennial ryegrass (lolium perenne). | epichloë typhina is a biotrophic fungal pathogen which causes choke disease of pooid grasses. the anamorphic state, acremonium typhinum, is placed in the section albo-lanosa along with related, mutualistic, seed-disseminated endophytes. as an initial study of gene structure and evolution in epichloë and related endophytes, the beta-tubulin gene, tub2, of the perennial ryegrass choke pathogen (etprg) was cloned and sequenced. the coding sequence and the predicted beta-tubulin amino acid sequence ... | 1990 | 2147581 |
| the effect of differing types of cloth and of contamination by non-feed nitrogen on intestinal digestion estimates using porous synthetic-fibre bags in a cow. | the influence of free surface area and pore size of a synthetic-fibre bag, and flow of intestinal substances into the bag, on the intestinal digestion estimates by the nylon-bag method of dry matter (dm), neutral detergent fibre (ndf), nitrogen, ndf-n and feed 15n was studied using a non-lactating cow fed on hay supplemented with barley and oats at maintenance level. the bags containing 15n-labelled ground ryegrass (lolium perenne), barley, barley straw or rapeseed (brassica napus) straw were in ... | 1990 | 2334660 |
| generation of anti-idiotypic and anti-anti-idiotypic monoclonal antibodies in the same fusion. support of jerne's network theory. | upon immunization of mice with a mab (290a-167) directed against an epitope of lol p i (the major allergenic determinant of lolium perenne), both anti-idiotypic (aid) mab (ab2) and anti-aid mab (ab3) were produced. the ab2 displayed the following internal image properties of lol p i: it can be affinity-purified on an immobilized id column; its binding to the anti-lol p i mab (290a-167) is inhibited by lol p i; it inhibits in a dose-response fashion the binding of the specific id to ag. it is rec ... | 1990 | 2341720 |
| detection of auto-anti-idiotypic antibodies to lol p i (rye i) ige antibodies in human sera by the use of murine idiotypes: levels in atopic and non-atopic subjects and effects of immunotherapy. | anti-idiotypic antibodies (anti-id abs) are involved in the regulation of a number of immune responses including the ige antibody production. in atopic patients, the increased synthesis of ige antibodies could be related to a defective production of regulatory anti-id abs. in the present study, we first developed a sensitive assay for measuring the levels of anti-id abs directed against antibodies specific for lol p i, the major allergenic determinant of lolium perenne (rye grass). in this assay ... | 1990 | 2372990 |
| the effect of fishmeal on the digestion of grass silage by growing cattle. | the effect of two levels of fishmeal substitution (50 (fm1) and 150 (fm2) g/kg) of a grass silage control diet (c) on the rumen digestion of organic matter and nitrogen, and the small intestinal disappearance of amino acids was examined in young growing cattle each equipped with simple pvc cannulas in the dorsal sac of the reticulo-rumen, the proximal duodenum and the terminal ileum. the silage was a primary growth of perennial ryegrass (lolium perenne) (+formic acid) with a total n content of 2 ... | 1990 | 2383528 |
| cdna cloning and immunological characterization of the rye grass allergen lol p i. | the complete amino acid sequence of two "isoallergenic" forms of lol p i, the major rye grass (lolium perenne) pollen allergen, was deduced from cdna sequence analysis. cdna clones isolated from a lolium perenne pollen library contained an open reading frame coding for a 240-amino acid protein. comparison of the nucleotide and deduced amino acid sequence of two of these clones revealed four changes at the amino acid level and numerous nucleotide differences. both clones contained one possible as ... | 1990 | 1697854 |
| decontamination of landfill leachate by soils with different textures. | soils with different textures (sandy, loamy, and clay soils) were used as filters to attenuate leachate from the gin drinkers' bay landfill. they were used to pack columns of different depths: 0.2, 0.6, and 1.0 m. eight millimeters of leachate was drained into the soil columns each day for 56 days. the percolated leachates were collected weekly and their properties analyzed. it was revealed that the properties became rather stable at day 28 and therefore only the data from day 28 are presented. ... | 1990 | 2096848 |
| mapping of lol p i allergenic epitopes by using murine monoclonal antibodies. | murine monoclonal antibodies (mabs) against three non-overlapping epitopes of lol p i allergen were previously produced and subsequently used for purification of the allergen. in the present study, these mabs were further characterized, and the biological activity of the purified allergen assessed. the three mabs were of the igg isotype and carried a kappa light chain. their affinity constants were in the range of 7.4-15.1 x 10(-9) mol/l. purified lol p i kept its biological activity, as shown b ... | 1989 | 2481823 |
| molecular genetics of human immune responsiveness to lolium perenne (rye) allergen, lol p iii. | lol p ii and iii are each about 11-kd protein allergens from the pollen of lolium perenne (rye grass). we have found that human immune responses (ige and igg antibodies) to both proteins are significantly associated with hla-dr3. in addition, the two proteins are cross-reactive with the antibodies in many human sera (about 84% human sera showed the cross-reactivity). we have determined greater than 90% of the amino acid sequences of the two proteins and found that they are at least 54% homologou ... | 1989 | 2496037 |
| complete primary structure of a lolium perenne (perennial rye grass) pollen allergen, lol p iii: comparison with known lol p i and ii sequences. | the complete amino acid sequence of a lolium perenne (rye grass) pollen allergen, lol p iii, determined by the automated edman degradation of the protein and its selected fragments, is reported in this paper. cleavage by enzymatic and chemical techniques established unambiguously the sequence for this 97-residue protein (mr = 10,909), which lacks cysteine and shows no evidence of glycosylation. the sequence of lol p iii is very similar to that of another l. perenne allergen, lol p ii, which was ... | 1989 | 2605214 |
| particle breakdown and rumen digestion of fresh ryegrass (lolium perenne l.) and lucerne (medicago sativa l.) fed to cows during a restricted feeding period. | 1. five rumen-fistulated cows were given freshly cut ryegrass (lolium perenne l.) and lucerne (medicago sativa l.), on separate occasions, to compare rates of particle breakdown and aspects of rumen fermentation. 2. cattle were required to consume their daily allowance (offered ad lib.) in two 2 h feeding periods daily. 3. during the first 2 h feeding period, dry matter (dm) intakes of ryegrass (4.75 kg) and lucerne (4.85 kg) were similar. eating reduced 46% of ryegrass and 61% of lucerne to a s ... | 1989 | 2706235 |
| human immune responsiveness to lolium perenne pollen allergen lol p iii (rye iii) is associated with hla-dr3 and dr5. | a well-characterized allergen of lolium perenne (perennial rye grass) pollen, lol p iii, has been used as a model antigen to study the genetic control of the human immune response. associations between hla type and ige or igg antibody (ab) responsiveness to lol p iii were studied in two groups of skin-test-positive caucasoid adults (n = 135 and 67). we found by nonparametric and parametric analyses that immune responsiveness to lol p iii was significantly associated with hla-dr3 and dr5. no asso ... | 1989 | 2715056 |
| theoretical considerations in the estimation of rumen fractional outflow rate from various sampling sites in the digestive tract. | the digesta markers 103ruthenium phenanthroline (ru-p) and 51cr-edta were continuously infused into the rumen of young lambs offered clover hay ad lib. indoors or grazing pure species swards of either lucerne (medicago sativa), white clover (trifolium repens), ryegrass (lolium perenne) or prairie grass (bromus catharticus). following the cessation of infusion the fractional outflow rate (for) of markers from the rumen was estimated from the rate of decline of marker concentration in rumen and ab ... | 1989 | 2789986 |
| allergenic fragments of ryegrass (lolium perenne) pollen allergen lol p iv. | to facilitate studies on establishing the nature of structure/function relationships of allergens, ryegrass pollen allergen, lol p iv, was cleaved into smaller fragments by cyanogen bromide (cnbr) and the resulting peptides were further digested with trypsin. the resulting peptides were then fractionated by high performance liquid chromatography (hplc) on a c-18 reverse phase column. the allergenic activity of the hplc fractions was evaluated in terms of their ability to inhibit the binding of 1 ... | 1989 | 2793222 |
| transfer of radiocesium from different environmental sources to ewes and suckling lambs. | indoor experiments are described that compare the transfer of radiocesium to ewe and lamb tissues from different sources. lactating ewes were fed either perennial ryegrass contaminated by chernobyl fallout, or saltmarsh vegetation contaminated by marine discharges from the sellafield reprocessing plant. the transfer to ewe tissues and milk was greater from the chernobyl contaminated herbage than from saltmarsh vegetation. lambs receiving a mixture of vegetation and milk were given radiocesium fr ... | 1989 | 2793473 |
| characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group i molecular weight range. | we have purified a 24/25 kd allergen from orchard grass pollen (dactylis glomerata) that has an allergenic potency similar to that of the major group i allergen. we provisionally named this allergen grass 4b1 after the monoclonal antibody used for its identification and purification. this monoclonal antibody was obtained by immunizing mice with whole lolium perenne-pollen extract and by screening the antibody producing hybrids for reactivity with dactylis glomerata-pollen extract. grass 4b1 is p ... | 1989 | 2913133 |
| kinetic aspects of calcium metabolism in lactating sheep offered herbages with different ca concentrations and the effect of protein supplementation. | 1. two experiments are described in which kinetic aspects of calcium metabolism were studied in housed lactating sheep consuming different fresh herbage species. the importance of protein supply was also investigated. 2. in expt. 1, two groups (n 4) were offered, ad lib., a freshly cut ryegrass (lolium perenne l.)-white clover (trifolium repens l.) pasture containing 5.48 g ca/kg dry matter (dm). one group was supplemented daily with 100 g protected casein. a third group (n 4) was offered, ad li ... | 1989 | 2923856 |
| faecal endogenous loss of calcium in young sheep. | 1. two groups of eight 6-7-month-old wether lambs were offered either a frozen ryegrass (lolium perenne l.)-white clover (trifolium repens l.) pasture or a ryegrass-white clover hay, containing 12.1 and 6.4 g calcium/kg dry matter (dm) respectively. within groups the amounts offered to individual sheep ranged from 0.5 to 2.0 times the estimated maintenance energy requirements. 2. a single intravenous injection of 150 microci 45ca as cacl2.2h2o, and stable balances were used to determine absorpti ... | 1989 | 2923857 |
| vegetation establishment on a deposit of zinc mine wastes. | field trials concerning the establishment of plant cover on a deposit of wastes from the ammeberg zinc mine in central sweden were carried out during 1976-1985. different soil conditioners and manures were applied and plant species cultivars were evaluated with regard to plant biomass, vigour, durability and content of zinc, lead and cadmium. sewage sludge and topsoil led to better establishment of grasses than did municipal waste, straw and hydraulic seeding. after 2 years, festuca rubra and po ... | 1989 | 15092483 |
| the effect of date of cut and barley substitution on gain and on the efficiency of utilization of grass silage by growing cattle. 1. gains in live weight and its components. | 1. a primary growth of perennial ryegrass (lolium perenne) was cut early or late to produce silages of high and low digestibility. the crops were wilted for 2-4 h and preserved with formic acid at 2.4 litres/t fresh weight. the resulting silages were well preserved with a ph of 3.9 and 3.8, lactic acid content of 108 and 73 g/kg dry matter (dm) and total nitrogen content of 24.6 and 18.4 g/kg dm for early- and late-cut silage respectively. 2. forty-two british friesian male castrates (steers) in ... | 1988 | 3196672 |
| the effect of date of cut and barley substitution on gain and on the efficiency of utilization of grass silage by growing cattle. 2. nutrient supply and energy partition. | 1. the effect of harvesting date of perennial ryegrass (lolium perenne) on the nutritive value of the resultant silage and the effect of substitution of late-cut silage with barley was examined in growing cattle. the diets comprised early-cut (h) and late-cut (l) silage offered alone or with 280 (lc1) or 560 (lc2) g rolled barley/kg total dry matter (dm) substituted for late-cut silage. 2. both silages were prepared with the addition of formic acid (850 milligram; 2.4 litres/t fresh weight) to a ... | 1988 | 3196673 |
| protein metabolism in the rumen of silage-fed steers: effect of fishmeal supplementation. | 1. ryegrass (lolium perenne cv. cropper) silage was given to four friesian steers (initial live weight (lw) 172 kg) alone or with a fishmeal supplement (150 g fresh weight/kg silage dry matter (dm)) in a balanced two-period change-over design. the dietary components were the same as those used in a recent experiment by gill et al. (1987). all diets were offered hourly at 24 g dm/kg lw. 2. fishmeal supplementation increased dietary nitrogen intake (p less than 0.01) and significantly increased th ... | 1988 | 3196674 |
| association of hla-dr3 with human immune response to lol p i and lol p ii allergens in allergic subjects. | associations between hla type and ige or igg antibody (ab) responses to two well-characterized, antigenetically non-crossreactive components of lolium perenne (rye grass) pollen extract, lol p i (rye i) and lol p ii (rye ii) were studied in two groups of skin-test positive (st+) caucasoid adults. by both nonparametric and parametric statistical methods, significant associations were found between ab responses to both lol i and lol ii and the possession of hla-dr3. in view of the well-known assoc ... | 1988 | 3261461 |
| in vitro synthesis of ige by human peripheral blood leucocytes: vi. seasonal variations in synthesis of rye pollen (lolium perenne) specific ige. | fractionation by percoll density centrifugation of peripheral blood leucocyte cells, from atopic subjects with seasonal hay fever, unmasked ige-b cell populations whose individual capacities to synthesize ige in vitro were obscured in cultures of unfractionated b cells. b cell cultures from all six subjects in the study released rye pollen-specific ige during the 6 days of culture, but actual synthesis was significant only in october, the pollen season. synthesis in october occurred most frequen ... | 1988 | 3263316 |
| standardization of rye-grass pollen (lolium perenne) extract. an immunochemical and physicochemical assessment of six candidate international reference preparations. | six candidate extracts of lolium perenne (rye-grass) pollen have been studied in 6 laboratories using a variety of immunochemical and physicochemical techniques. radioallergosorbent test inhibition, crossed immunoelectrophoresis, crossed radio-immunoelectrophoresis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis combined with immunoblot, thin-layer isoelectric focusing and enzyme-linked immunosorbent assay inhibition were used to evaluate each of the coded extracts. the source materi ... | 1988 | 3372043 |
| large particle breakdown by cattle eating ryegrass and alfalfa. | the proportion of large particles (lp) broken down to small, insoluble particles by primary mastication (eating), rumination, digestion and detrition (rubbing) was determined for separated leaf and stem fractions of perennial ryegrass (lolium perenne) and alfalfa (medicago sativa) fed to cattle cannulated at the esophagus. large particles were defined as those particles retained during wet sieving on a screen with an aperture of 1.18 mm. reduction in weight of particles caused by solubilizing or ... | 1988 | 3378956 |
| the digestion of perennial ryegrass (lolium perenne cv. melle) and white clover (trifolium repens cv. blanca) by grazing cattle. | 1. pure swards of perennial ryegrass (lolium perenne cv. melle) as a primary growth (may), trimmed primary growth (early june) and regrowth (late june), and white clover (trifolium repens cv. blanca) as a mature primary growth (july) and vegetative regrowth (august), were grazed by twelve friesian steers (mean body-weights throughout experiment 152-231 kg) at daily allocation rates of forage which provided dry matter (dm) intakes ranging from 28 to 36 g/kg body-weight). 2. total nitrogen content ... | 1988 | 3408697 |
| study of the epitope structure of purified dac g i and lol p i, the major allergens of dactylis glomerata and lolium perenne pollens, using monoclonal antibodies. | the use of mab allowed us to further analyze the cross-reactivity between purified dac g i and lol p i, the major allergens of dactylis glomerata (cocksfoot) and lolium perenne (rye grass), respectively. it was first shown, using ief, followed by immunoprinting, that serum ige antibodies from most grass-sensitive patients recognize both dac g i and lol p i. second, three different anti-lol p i mab, 290a-167, 348a-6, and 539a-6, and one anti-dac g i mab, p3b2 were all shown to react with dac g i ... | 1988 | 2460536 |
| a study of the human immune response to lolium perenne (rye) pollen and its components, lol p i and lol p ii (rye i and rye ii). ii. longitudinal variation of antibody levels in relation to symptomatology and pollen exposure and correction of seasonally elevated antibody levels to basal values. | this study used a standardized, dialyzed, lolium perenne (ryegrass) pollen extract and two of its well-characterized components, lol p i (rye i) and lol p ii (rye ii), to characterize the longitudinal variation of both ige and igg antibody (ab) levels, as well as total serum ige levels, in 20 grass-allergic subjects followed for 13 months. ab levels declined toward a basal level just before, and increased just after, the grass-pollination season, returning to the same basal level just before the ... | 1987 | 3680810 |
| the prevalence of allergic skin test reactivity to eight common aeroallergens in the u.s. population: results from the second national health and nutrition examination survey. | immediate hypersensitivity skin tests to eight select allergens were performed on a sample (n = 16,204) of the civilian noninstitutional population of the united states, 6 to 74 years of age, in the second national health and nutrition examination survey (nhanes ii). the eight allergens were house dust, cat, dog, alternaria, mixed giant/short ragweed, oak, perennial ryegrass, and bermuda grass. skin test reactivity was defined as a mean erythema diameter greater than or equal to 10.5 mm at the 2 ... | 1987 | 3680811 |
| immunochemical studies of lolium perenne (rye grass) pollen allergens, lol p i, ii, and iii. | it was reported earlier that human immune responses to three perennial rye grass (lolium perenne) pollen allergens, lol p i, ii, and iii, are associated with histocompatibility leukocyte antigen (hla)-dr3. rye-allergic people are often concordantly sensitive to all three of these allergens. since earlier studies suggested that these antigens are non-cross-reactive, their immunologic relatedness by double antibody radioimmunoassay (daria) was studied in order to understand further the immunochemi ... | 1987 | 3693899 |
| activation of human serum complement with allergens. i. generation of c3a, c4a, and c5a and induction of human neutrophil aggregation. | to understand the relevance of allergens in generation of c3a, c4a, and c5a in normal human serum, we studied extracts of several allergens (house dust, house dust mite, aspergillus fumigatus, and perennial ryegrass). known complement activators zymosan and endotoxin were used as controls. generation of c3a, c4a, and c5a (determined by radioimmunoassays) occurred with extracts of house dust and aspergillus more than with house dust mite and ryegrass. anaphylatoxins were produced both in dose and ... | 1987 | 3496373 |
| digestibility, intake and mineral utilization of combinations of grasses and legumes by lambs. | defined mixtures of two grass (orchardgrass, perennial ryegrass) and two legume (alfalfa, red clover) hays, harvested in two cuttings in 2 yr, were fed in digestibility, intake and mineral balance trials to wether lambs. each mixture was fed in ad libitum amounts to six crossbred wether lambs weighing 35 to 45 kg. the effect of increasing proportion of legume in mixtures on dry matter digestibility (dmd), neutral detergent fiber digestibility (dndf) and digestible dry matter intake (ddmi) differ ... | 1987 | 3597188 |
| an investigation of human immune response to perennial ryegrass (lolium perenne) pollen cytochrome c (lol p x). | cytochrome c (lol p x) was purified from the pollen of perennial ryegrass (lolium perenne). it was used in a sensitive double-antibody radioimmunoassay to determine specific igg and ige antibody (ab) levels in the sera of grass-allergic human subjects and the relationship between immune responsiveness and hla type. a total of 139 subjects were studied, including 63 subjects treated by grass immunotherapy and 76 untreated subjects. the sera of only four subjects were found to contain anticytochro ... | 1987 | 3038988 |
| the use of monensin or formaldehyde to control the digestion of the nitrogenous constituents of perennial ryegrass (lolium perenne cv. melle) and white clover (trifolium repens cv. blanca) in the rumen of cattle. | pure swards of perennial ryegrass (lolium perenne cv. melle) and white clover (trifolium repens cv. blanca) were harvested daily as primary growth (grass in may, clover in june) or regrowths (clover only in july) and offered, in the long form, to growing cattle at a feeding rate of 22 g dry matter/kg live weight. with each forage, two treatments were compared with the untreated forage (c): monensin (100 g active ingredient/kg, 250 mg/d) addition to the rumen (treatment m) or formaldehyde (30 g/k ... | 1987 | 3801385 |
| distribution of a major allergen of rye grass (lolium perenne) pollen between other grass species. | polyacrylamide gel electrophoresis in sodium dodecyl sulphate (sds) followed by protein staining has shown that extracts from 11 different grass pollens contained proteins with similar molecular weights to that of the allergen r7 from rye grass (lolium perenne) pollen extract (i.e. 29,000-31,000 daltons). western blotting and detection with polyclonal (rabbit) antibodies raised against the purified r7 (rye i) allergen indicated that these proteins were antigenically related and their allergenic ... | 1987 | 2437060 |
| effects of fungal endophytes on the seed and seedling biology of lolium perenne and festuca arundinacea. | many grasses are infected by endophytic fungi that grow intercellularly in leaves, stems, and flowers and are transmitted maternally by hyphal growth into ovules and seeds. the seed biology and seedling growth of endophyte-infected and uninfected perennial ryegrass (lolium perenne) and tall fescue (festuca arundinacea) were investigated under controlled environmental conditions. the percentage of filled seeds produced by infected tall fescue was over twice of uninfected tall fescue; infected and ... | 1987 | 28311516 |
| effects of ambient levels of air pollution on grass swards subjected to different defoliation regimes. | the urgent need to evaluate effects of air quality on plant growth in the field is discussed in relation to the difficulties which field-based studies pose in the interpretation of data. an initial study is described which aimed to overcome some of these difficulties and the data are discussed within the remaining limitations. the productivity of pure swards of lolium perenne l. and dactylis glomerata l., subjected to defoliation at different times of the year, was studied at field sites with di ... | 1987 | 15092760 |
| cross-reactive and unique grass group i antigenic determinants defined by monoclonal antibodies. | as part of a study to probe the immunochemical basis for allergenic cross-reactivity among grass pollens, a series of useful reagents has been prepared. the major grass-pollen allergen, designated group i (gpi), was isolated from five grass pollens (meadow fescue, june grass, sweet vernal grass, redtop grass, and perennial ryegrass). the purified gpi antigens were used to immunize individual groups of balb/c mice. a total of 123 hybridoma-derived anti-gpi monoclonal antibodies (mabs) was produce ... | 1987 | 2434551 |
| metabolism of lactic acid isomers in the rumen of silage-fed sheep. | 1. four mature sheep were offered perennial ryegrass (lolium perenne, cv. s23) silage (885 g dry matter/d) at hourly intervals. the silage was well fermented with a ph of 4.0, a lactic acid content of 139 g/kg dry matter and an organic matter digestibility of 0.766. 2. continuous intraruminal infusions of 14c-labelled sodium salts of [u-14c]acetic acid, [2-14c]propionic acid, [2-14c]butyric acid and d- and l-[u-14c]lactic acid and an intravenous infusion of [u-14c]glucose were made on separate o ... | 1986 | 3676167 |
| the efficiency of energy utilization in growing cattle consuming fresh perennial ryegrass (lolium perenne cv. melle) or white clover (trifolium repens cv. blanca). | 1. twenty friesian steers (225 kg live weight) were fed on mid- (m) (june-july) and late- (l) (august-september) season crops of either fresh perennial ryegrass (lolium perenne cv. melle) (g) or white clover (trifolium repens cv. blanca) (c). each of the forage diets was offered at three restricted planes of nutrition above maintenance to compare the effect of forage species on the efficiency of energy utilization. all diets were harvested daily from swards of regrowth forage of intended equival ... | 1986 | 3676184 |
| microbially corrected amino acid composition of rumen-undegraded feed protein and amino acid degradability in the rumen of feeds enclosed in nylon bags. | 1. in the previous work (varvikko & lindberg, 1985), 15n-labelled rapeseed (brassica napus), barley, ryegrass (lolium perenne) and barley straw were incubated in the rumen in nylon bags for 5, 12 and 24 h and microbial nitrogen in the residues was quantified using the feed 15n-dilution method. in the present study, residual amino acids (aa) of these feeds were analysed, and microbially corrected aa of feed origin (feed aa) were estimated as the difference between total residual aa and respective ... | 1986 | 3676189 |
| effect of forage species and season on nutrient digestion and supply in grazing cattle. | 1. a total of twenty friesian steers were grazed on pure swards of either perennial ryegrass (lolium perenne cv. melle) or white clover (trifolium repens cv. blanca) from may to late august to examine the effect of forage species and season on nutrient digestion and supply. within each forage species, two daily allowances of forage (i.e. 30 and 60 g dry matter (dm)/kg live weight) were given, and nutrient flow into the small intestine was measured on thirteen separate occasions (viz. seven grass ... | 1986 | 3676197 |
| the effect of forage species and stage of harvest on the processes of digestion occurring in the rumen of cattle. | 1. pure swards of perennial ryegrass (lolium perenne l. cv. melle) or white clover (trifolium repens l. cv. blanca) were harvested daily as either primary growth (may-june) or mid- (july) and late- (august-september) season 4-week regrowths and offered to friesian steers at two levels of feed allowance (18 and 24 g dry matter (dm)/kg live weight), to examine the effect of forage species and stage of harvest on nutrient digestion and supply. 2. the early- and mid-season grasses had low nitrogen ( ... | 1986 | 3676223 |
| the effect of digestibility and forage species on the removal of digesta from the rumen and the voluntary intake of hay by sheep. | 1. the characteristics of digestion, passage and rumen fill of three hays: early- and late-cut perennial ryegrass (lolium perenne cv. endura) and white clover hay (trifolium repens cv. blanca and pronitro) were studied using six rumen-cannulated sheep fed at a restricted level of intake (18 g dry matter (dm)/kg live weight (lw) per d), in a two 3 x 3 latin square design. 2. voluntary intake of the same diets was measured using a further six non-cannulated sheep in a similar design. 3. rate of di ... | 1986 | 3676225 |
| the effect of supplementation with maize starch and level of intake of perennial ryegrass (lolium perenne cv. endura) hay on the removal of digesta from the rumen of sheep. | 1. eight wether sheep were offered a diet of perennial ryegrass (lolium perenne cv. endura) hay once daily at two levels of intake (11 and 16.5 g dry matter (dm)/kg live weight (lw] with or without maize starch (175 g dm/kg hay dm). the experiment consisted of four periods, each lasting 32 d. rates of digestion of the hay were measured by incubation in dacron bags and rates of passage using chromium-mordanted hay. rumen pool sizes of dm, organic matter and fibre were measured by emptying the rum ... | 1986 | 3676226 |
| physicochemical and immunochemical characterization of allergenic proteins from rye-grass (lolium perenne) pollen prepared by a rapid and efficient purification method. | three fractions of rye-grass (lolium perenne) pollen extract have been isolated by preparative isoelectric focusing (i.e.f.) and characterized in terms of physicochemical and immunochemical properties. the purified components were designated 'r7' and 'r14' on the basis of their positions in relation to other rye-grass pollen extract components on sds/polyacrylamide-gel electrophoresis and their apparent molecular masses were assessed as 31 and 11 kda respectively. on i.e.f., r14 split into two c ... | 1986 | 3718469 |
| a study of the human immune response to lolium perenne (rye) pollen and its components, lol p i and lol p ii (rye i and rye ii). i. prevalence of reactivity to the allergens and correlations among skin test, ige antibody, and igg antibody data. | in a stratified random sample of 320 white adults, the prevalence of puncture skin test positivity (st +) to lolium perenne (rye grass)-pollen extract (lpe) was 16%. fifteen percent of all subjects (or 84% of subjects classified lpe ige antibody positive [ab +]) was classified ige ab + to highly purified lol p i (rye i), and 4% of all subjects (or 26% of subjects classified lpe ige ab +) was classified ige ab + to highly purified lol p ii (rye ii). these data and similar results obtained in an a ... | 1986 | 3782681 |
| cerebellar disease in an adult cow. | this is the report of clinical signs and lesions of a cerebellar disorder in an adult four year old limousin cow grazing perennial ryegrass (lolium perenne). the most striking histopathological lesion was a marked paucity of purkinje cells throughout the cerebellum. | 1986 | 17422607 |
| studies on the host range, biology, and pathogenicity of punctodera punctata infecting turfgrasses. | punctodera punctata completed its life cycle on poa annua (annual bluegrass), p. pratensis (merion kentucky bluegrass), lolium perenne (perennial ryegrass), and festuca rubra rubra (spreading fescue). minimum time for completion of a life cycle from second-stage juvenile to mature brown cyst was 40 days at 22-28 c. inoculation by single juveniles indicated that reproduction was most likely by amphimixis. infestation levels of 50 or 500 juveniles/250 cm(3) soil did not affect top dry weight, root ... | 1985 | 19294077 |