Publications

structure and expression of three genes encoding acc oxidase homologs from melon (cucumis melo l.).	the enzyme acc oxidase catalyses the last step of ethylene biosynthesis in plants, converting 1-aminocyclopropane-1-carboxylic acid (acc) to ethylene. we have previously described the isolation and characterization of a cdna clone (pmel1) encoding an acc oxidase homolog from melon (cucumis melo l.). here we report the isolation and characterization of three genomic clones, corresponding to three putative members of the acc oxidase gene family in melon. all are transcriptionally active. the seque ...	1996	8628251
beta-carotene content of postharvest orange-fleshed muskmelon fruit: effect of cultivar, growing location and fruit size.	the influence of two growing locations (soil types), six fruit sizes, and two years on the postharvest beta-carotene content of muskmelon (cucumis melo l. var. reticulatus naud.) fruit was studied with two different cultivars. fully abscised commercial size fruit: 9, 12, 15, 18, 23, and 30 (fruit/0.04 m3 shipping box) had highly variable beta-carotene contents (5.3 to 33.8 micrograms/g fresh weight) that varied by size class, soil type and cultivar. beta-carotene content increased with fruit siz ...	1996	8865328
raffinose oligosaccharide concentrations measured in individual cell and tissue types in cucumis melo l. leaves: implications for phloem loading.	raffinose, stachyose, and galactinol are synthesized in intermediary cells (specialized companion cells) of the minor-vein phloem of cucurbits. to better understand the role of these carbohydrates and the regulation of their synthesis and transport, we measured the concentrations of each of the components of the raffinose oligosaccharide synthetic pathway in mesophyll and sieve element-intermediary cell complexes (se-iccs) in the leaves of melon (cucumis melo l. cv. hale's best jumbo). these con ...	1996	28321672
expression of acc oxidase antisense gene inhibits ripening of cantaloupe melon fruits.	the plant hormone ethylene plays a major role in the ripening of climacteric fruit. we have generated transgenic cantaloupe charentais melons expressing an antisense acc oxidase gene; acc oxidase catalyzes the last step of ethylene biosynthesis. ethylene production of transgenic fruit was < 1% of control untransformed fruit, and the ripening process was blocked both on and off the vine. the antisense phenotype could be reversed by exogenous ethylene treatment. analysis of antisense acc oxidase m ...	1996	9631011
improved isolation, stability and substrate specificity of cucumisin, a plant serine endopeptidase.	cucumisin (ec 3.4.21.25), a serine endopeptidase, was isolated by a simple purification procedure from the prince melon (cucumis melo ssp. melo, cv. 'prince melon'). the enzyme is stable over a wide ph range (4-11) and to heat, 80% of its initial activity remaining even at ph 11.1 and at 60 degrees c for 20 min. the enzyme was inactive at 72 degrees c and ph 8.0, but 38% of the activity remained in the presence of 10% (w/v) glycerol. caseinolysis by cucumisin indicated full activity in 8 m urea ...	1995	7576259
selection of somaclonal variants with low-temperature germinability in melon (cucumis melo l.).	plants were regenerated from adventitious buds and somatic embryos (r0) of melon (cucumis melo l.), the cultivar andes. somaclonal variants of melon with low temperature germinability were selected from the progenies (r1) of r0 plants. among 5,618 r1 seeds harvested from 23 r0 plants that were regenerated from adventitious buds 4 seeds germinated after 5 days of culture at 15 °c (selection rate; 0.07%). however, among 374 r2 seeds harvested from 2 r1 plants no seed germinated after 7 days of cul ...	1995	24186622
poisoning of cattle attributed to cucumis melo ssp agrestis (ulcardo melon).		1995	8534234
amino acid sequences of trypsin inhibitors from the melon cucumis melo.	two squash family trypsin inhibitors, cmeti-a and cmeti-b, were isolated from the melon (cucumis melo) seeds, by ion exchange chromatography, gel filtration, affinity chromatography, and high-performance liquid chromatography, and their amino acid sequences were determined. all inhibitors contain 29 amino acid residues including 6 half-cystine residues. they differ by twelve amino acid residues. these polypeptides are strong inhibitors of bovine trypsin, with ki values of 1.6 x 10(-10) m (cmeti- ...	1995	8537309
[isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis].	fusarium oxysporum f.sp. melonis, a pathogen of melon (cucumis melo l.), was grown in shaken cultures at 26 degrees c in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. the extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. this ...	1995	8850131
a new procedure for the preparation of highly active melonin from the dry seeds of cucumis melo l.	melon (cucumis melo l.) dry seeds contain melonin, a protein that strongly inhibits ribosomes from different prokaryotic and eukaryotic sources including those from melon. the protein was purified by a new method to yield highly active and stable protein preparations that involves chromatography through s-sepharose fast flow, cm-sepharose, superdex 75 and mono-s. melonin shows important functional properties: 1) its inhibitory effects on translation were irreversible; 2) it is a single unglycosy ...	1995	7787738
characterization of the formae speciales of fusarium oxysporum causing wilts of cucurbits by dna fingerprinting with nuclear repetitive dna sequences.	the genetic relatedness of five formae speciales of fusarium oxysporum causing wilts of cucurbit plants was determined by dna fingerprinting with the moderately repetitive dna sequences folr1 to folr4. the four folr clones were chosen from a genomic library made from f. oxysporum f. sp. lagenariae 03-05118. total dnas from 50 strains representing five cucurbit-infecting formae speciales, cucumerinum, melonis, lagenariae, niveum, and momordicae, and 6 strains of formae speciales pathogenic to oth ...	1994	8085813
in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline.	the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ...	1994	8206443
protease from the sarcocarp of trichosanthes bracteata.	a protease has been purified from sarcocarp of trichosanthes bracteata (lam.) voigt by four steps of chromatography. its m(r) was estimated by sds-page to be ca 67,000. the optimum ph of the enzyme was 11 at 35 degrees using casein substrate. the enzyme was strongly inhibited by di-isopropyl fluorophosphate, but not by edta and cysteine protease inhibitors. the oxidized insulin b-chain was cleaved at the peptide bonds of cys7 (so3h)-gly8, glu13-ala14, try16-leu17 by the enzyme for 1 min. the res ...	1994	7764589
cusativin, a new cytidine-specific ribonuclease accumulated in seeds of cucumis sativus l.	dry seeds of cucumis sativus l. were found to contain a heat-sensitive endoribonuclease of a novel type which we have named cusativin. it was purified to apparent electrophoretic homogeneity by chromatography through s-sepharose fast flow, sephadex g-75, cm-sepharose, superdex 75-fplc (fast protein liquid chromatography) and mono s-fplc. it is a single unglycosylated polypeptide chain with an apparent molecular mass (m(r)) of 22900. polyclonal anti-cusativin antibodies raised in rabbits only rea ...	1994	7765423
agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro).	cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ...	1994	24193640
cucumisin, a serine protease from melon fruits, shares structural homology with subtilisin and is generated from a large precursor.	cucumisin is a thermostable alkaline serine protease that is found in the juice of melon fruits (cucumis melo l.). we have determined the complete nucleotide sequence of a cucumisin cdna (2,552 nucleotides) and deduced the corresponding amino acid sequence. the open reading frame of the cdna consists of 731 codons and encodes a large precursor (molecular weight, 78,815) relative to the observed size of mature native cucumisin (67 kda). sequence comparisons reveal that cucumisin has several featu ...	1994	7806492
ploidy of somatic embryos and the ability to regenerate plantlets in melon (cucumis melo l.).	the number of chromosomes in cells of callus, somatic embryos and regenerated plantlets during somatic embryogenesis were examined in two cultivars of melon (cucumis melo l.). somatic embryos were diploid (50.0%/32.1%), tetraploid (38.5%/57.5%) and octoploid (11.5%/10.4%) whereas in callus cells diploidy (41.9%/43.3%), tetraploidy (27.9%/25.8%), octoploidy (11.6%/15.5%) and a low frequency of other types of ploidy and aneuploidy were observed. mixoploid somatic embryos were not observed. these r ...	1994	24192875
isoelectric focusing in immobilized ph gradient of melon (cucumis melo l.) seed protein: methodical and genetic aspects, and application in breeding.	genetic variability of melon seed proteins was studied by separation of seed protein by isoelectric focusing in immobilized ph gradient (ief-ipg) under denaturing conditions. a routine procedure was developed for ief-ipg of hundreds of individual melon seeds per day. a group of 74 accessions from 19 morphologically distinct groups and from different geographic origin were studied by ief-ipg using ph gradients of 4-10, 4-7 and 6-10. the electrophoretic analysis of the 74 accessions showed 270 rep ...	1994	7720692
a phenylalanine ammonia-lyase gene from melon fruit: cdna cloning, sequence and expression in response to development and wounding.	phenylalanine ammonia-lyase (pal) is the first enzyme of phenylpropanoid biosynthesis involved in the synthesis of a multiplicity of plant natural products. we have isolated and characterized a nearly full-length cdna clone (pmpal-1) corresponding to a melon fruit (cucumis melo l. var. reticulatus) gene coding for a protein which is highly similar to pal from other plants. melon fruit pal is transcriptionally induced both in response to fruit ripening and wounding. pal gene expression follows th ...	1994	7948894
quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (cucumis melo l.) root cells.	the presence of ca2+ ions in solution is vital for root growth. the plasma membrane is one of the first sites where competition between ca2+ and other ions occurs. we studied the competition between ca2+ and na+ or mg2+ for sorption sites on the plasma membrane of melon root cells. sorption of 45ca2+ to right-side-out pm vesicles of melon (cucumis melo l.) roots (prepared by aqueous two-phase partitioning) was studied at various ca2+ concentrations, in the presence of increasing concentrations o ...	1994	8189432
protease d from the sarcocarp of honeydew melon fruit.	a serine protease was purified from commercially available honeydew melon fruit (cucumis melo l. var. inodorus naud) by five steps including cm-sepharose cl-6b column chromatography. at the first cm-sepharose cl-6b chromatography four active fractions appeared. no difference in enzymatic properties and m(r) in each fraction was found. finally, honeydew melon protease d was isolated from the major active fraction d. a m(r) of 50,000 was determined for protease d on sds-page and gel filtration. pr ...	1993	7764026
physiological effects of ozone on cultivars of watermelon (citrullus lanatus) and muskmelon (cucumis melo) widely grown in spain.	two cultivars of watermelon (citrullus lanatus) and muskmelon (cucumis melo), which are widely grown in spain, were exposed to ozone (70 nl litre(-1), 6 h d(-1)) for 21 days. ozone sensitivity was assessed by recording the extent of visible injury, changes in fast-fluorescence kinetics, the relative-growth rate (r) of root (rr) and shoot (rs), and effects on the number of flowers produced per plant. leaf gas exchange was measured in order to provide some indication of the factors underlying diff ...	1993	15091805
genetic engineering of potyvirus resistance using constructs derived from the zucchini yellow mosaic virus coat protein gene.	three versions of the zucchini yellow mosaic virus (zymv) coat protein gene were engineered for expression in plants: the full-length coat protein sequence, the conserved core portion of the gene, and an antisense version. these constructs were introduced into muskmelon (cucumis melo) and tobacco plants (nicotiana tabacum) via agrobacterium tumefaciens-mediated transformation; gene expression was verified by northern and western analysis. transgenic r0 and r1 muskmelon plants expressing the full ...	1993	8324251
isolation of a ripening and wound-induced cdna from cucumis melo l. encoding a protein with homology to the ethylene-forming enzyme.	a cdna clone (pmel1) was isolated from a climacteric melon fruit cdna library using the tomato ethylene-forming-enzyme (efe) cdna, ptom13, as a probe. northern analysis of rna isolated from wounded leaf and fruit tissue and from preclimacteric and climacteric pericarp tissue was used to determine the pattern of pmel1 rna expression. pmel1 hybridized to a 1.3-kb transcript in climacteric fruit and wounded leaf tissue but was undetectable in preclimacteric fruit and unwounded leaves. maximal expre ...	1993	8444161
partial characterization of the translational inhibitor present in seeds of cucumis melo l.		1992	1486983
induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (cucumis melo l.).	a procedure leading to the regeneration of whole plants from protoplasts of melon is described. protoplasts were isolated from cotyledons and leaves of plants grown in vitro. after 14 days of culture, average viability and division rates were respectively 60% and 30% for the two organs, considering total initial protoplasts plated. the manipulation of the exogenous auxin / cytokinin balance in regeneration media enabled to direct morphogenesis towards somatic embryogenesis (1 mg·l(-1) 2,4-dichlo ...	1992	24201728
the role of beta-galactosidases in the modification of cell wall components during muskmelon fruit ripening.	the changes in activities of soluble beta-galactosidase and two forms of wall-bound beta-galactosidases extracted with nacl and edta were investigated throughout the development of muskmelon (cucumis melo l. cv prince) fruits. deae-cellulose ion-exchange chromatography of soluble beta-galactosidase revealed the presence of two isoforms. soluble isoform i was detected in all stages throughout the fruit development, whereas soluble isoform ii appeared around 34 d after anthesis when fruit ripening ...	1992	16653123
amino acid sequences of trypsin inhibitors from oriental pickling melon (cucumis melo l. var. conomon makino) seeds.	three inhibitors (cmcti-i, ii, and iii) were isolated from oriental pickling melon (cucumis melo l. var. conomon makino) seeds by acetone precipitation, gel filtration, and reversed phase chromatography. the amino acid sequences of these inhibitors were: [table; see text] the reactive sites (p1 and p1' sites) of these inhibitors are presumed to be the lys-ile indicated by an arrow, comparing them with other squash family inhibitors. all three inhibitors can inhibit lysyl endopeptidase and trypsi ...	1992	1368838
patterns of assimilate production and translocation in muskmelon (cucumis melo l.) : ii. low temperature effects.	muskmelon (cucumis melo l.) plants were exposed to a 10 degrees c chilling treatment for 72 hours, which induced leaf chilling injury symptoms (wilting, appearance of water-soaked areas, necrosis). chilling caused an accumulation of starch, sucrose, hexoses (glucose and fructose), and certain amino acids (glutamate, aspartate, and citrulline) in source leaf tissues, but no accumulation of stachyose or other galactosyl-oligosaccharides occurred. chilling also caused a general increase in sugar (s ...	1992	16669026
patterns of assimilate production and translocation in muskmelon (cucumis melo l.) : i. diurnal patterns.	continuous monitoring of steady-state carbon dioxide exchange rates in mature muskmelon (cucumis melo l.) leaves showed diurnal patterns of photosynthesis and respiration that were translated into distinct patterns of accumulation and phloem export of soluble sugars and amino acids. leaf soluble sugar patterns in general followed the pattern of photosynthetic activity observed in the leaf, whereas starch accumulated steadily throughout the light period. sugar and starch levels declined through t ...	1992	16669025
evaluation of restriction fragment length polymorphism in cucumis melo.	the objectives of this study were to assess the degree of restriction fragment length polymorphism (rflp) in cucumis melo and to determine interrelationships among cultivated varieties. initial screening of a genomic psti library revealed that approximately 40% of the clones were repetitive. a total of 162 unique and low-copy sequence clones were hybridized to seven diverse accesions of c. melo and a c. sativus cultivar 'pacer' to evaluate rflp variation. of these, 130 probes (80%) detected a po ...	1992	24202522
tolerance to a whitefly-transmitted virus causing muskmelon yellows disease in spain.	muskmelon yellowing disease was one of the most serious problems affecting muskmelon crops along the south-east coast of spain throughout the 1980s. the causal agent of this disease is a virus that we call muskmelon yellows virus (myv); myv is transmitted by the greenhouse whitefly trialeurodes vaporariorum westwood. it has proven impossible to find sources of resistance to myv within a wide collection of spanish muskmelon landraces and exotic varieties. however, 'nagata kin makuwa' and pi 16137 ...	1992	24201359
myrotoxins from a plant pathogenic isolate ofmyrothecium roridum.	an isolate ofm roridum (atcc 52485) which is a potent pathogen to muskmelon was shown to produce a series of macrocyclic trichothecenes, the myrotoxins, heretofore found to be produced only by an. isolate ofm roridum which is a pathogen to tomato. the fact that these two isolates are virulent pathogens and both produce the same potent mycotoxins suggests that these novel trichothecenes may play an important role in the pathogenicity of the fungi.	1991	23605653
distribution and immunolocalization of stachyose synthase in cucumis melo l.	indirect evidence for the site of stachyose biosynthesis has been provided by determining the occurrence and distribution of stachyose, raffinose and galactinol, the donor of the galactosyl moiety for stachyose synthesis, in cucumis melo l. cv. ranjadew. studies of enzyme activities for the synthesis of these sugars and their distribution in different plant organs and isolates has led to the conclusion that stachyose is synthesized mainly in mature leaves and seeds. nevertheless, stachyose-synth ...	1991	24186524
regulation of organogenesis and somatic embryogenesis by auxin in melon, cucumis melo l.	various tissues of seeds and seedlings of melon were cultured in vitro to study the effects of auxin concentration on organogenesis and embryogenesis. adventitious shoots and somatic embryos were formed from explants of cotyledons of mature seeds, hypocotyls of seedlings, and leaves and petioles of young plantlets. expanded cotyledons of seedlings formed only adventitious shoots. all tissues responded similarly to the 2,4-d concentration in the media, that is, adventitious shoots were formed at ...	1991	24221584
stachyose synthesis in mature leaves of cucumis melo. purification and characterization of stachyose synthase (ec 2.4.1.67).	galactinol: raffinose-6-galactosyltransferase (ec 2.4.1.67), a stachyose synthase, was extracted from mature leaves of cucumis melo cv. ranjadew and was purified to homogeneity by (nh4)2so4 precipitation, ion-exchange chromatography, gel-filtration and non-denaturing polyacrylamide gel electrophoresis. a specific activity of 516 μkat · mg(-1) and a 160-fold purification was achieved. the ph optimum of the enzyme reaction was found to be 6.8 in sodium-phosphate buffer, and the temperature optimum ...	1991	24194243
acid and neutral invertases in the mesocarp of developing muskmelon (cucumis melo l. cv prince) fruit.	acid and neutral invertases were found in the mesocarp of developing muskmelon (cucumis melo l. cv prince) fruit and the activities of these enzymes declined with maturation of the fruit, concomitantly with the accumulation of sucrose. neutral invertase was only present in the soluble fraction and acid invertase was present in both the soluble and cell-wall fractions. the cell-wall fraction contained three types of acid invertase: a nacl-released invertase; an edta-released invertase, and a tigh ...	1991	16668269
eggshell morphology of the small eggs of human trematodes in thailand.	light and scanning electron micrographs of opisthorchis viverrini, haplorchis taichui, h. pumilio, a phaneropsolus bonnei, and prosthodendrium molenkampi eggs were studied. under light microscopy. o. viverrini eggs had rough eggshells and prominent shoulders. h. taichui, h. pumilio were similar in shape and had smooth eggshells and prominent shoulders. h. pumilio eggs were bigger than h. taichui eggs. p. bonnei and p. molenkampi eggs had smooth eggshells and indistinct shoulders. p. bonnei eggs ...	1991	1820654
temperature-sensitive replication of cucumber mosaic virus in muskmelon (cucumis melo cv. iroquois), maps to rna 1 of a slow strain.	several strains of cucumber mosaic virus have been categorized as either 'fast' or 'slow', based on the time of appearance of symptoms after inoculation onto zucchini squash (curbita pepo cv. black beauty). these strains were examined for their ability to replicate in muskmelon (cucumis melo cv. iroquois) at elevated temperatures. all of the fast strains were able to replicate at 37 degrees c in muskmelon, whereas all of the slow strains were unable to replicate to detectable levels at 37 degree ...	1991	1856702
toxicity of potassium cyanide added to fresh fruit and juice.	to investigate the toxicity of potassium cyanide in fresh fruit and juice, male and female wistar rats were orally dosed with fruit homogenates or juices containing 3 x ld50 of potassium cyanide. these were given in single doses at various intervals after spiking. the dosing solutions were analysed for cyanide using a cyanide test kit. there was a good correlation between the toxic signs in rats and the cyanide remaining in dosing solutions. the toxicity of spiked apple and honeydew melon dimini ...	1991	1959821
differential accumulation of hydroxyproline-rich glycoproteins in bean root nodule cells infected with a wild-type strain or a c4-dicarboxylic acid mutant of rhizobium leguminosarum bv. phaseoli.	an antiserum raised against deglycosylated hydroxyproline-rich glycoproteins (hpgps) from melon (cucumis melo l.) was used to study the relationship between rhizobium infection and induction of hrgps in bean (phaseolus vulgaris l.) root nodule cells infected with either the wild-type or a c4-dicarboxylic acid mutant strain of rhizobium leguminosarum bv. phaseoli. in effective nodules, where fixation of atmospheric dinitrogen is taking place, hrgps were found to accumulate mainly in the walls of ...	1991	24194237
agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants.	transgenic muskmelon (cucumis melo l.) plants were produced efficiently by inoculating cotyledon explants with agrobacterium tumefaciens strain lba4404 bearing a ti plasmid with the npt ii gene for kanaymcin resistance. after co-cultivation for three days, expiants were transferred to melon regeneration medium with kanamycin to select for transformed tissue. shoot regeneration occurred within 3-5 weeks; excised shoots were rooted on medium containing kanamycin before transferring to soil. morpho ...	1990	24226603
species determination of eggs of opisthorchiid and heterophyid flukes using scanning electron microscopy.	eggs of opisthorchiid flukes have very characteristic muskmelon-like surface structure of their shells and can be differentiated using this mark from eggs of heterophyid flukes. surface structure and morphological details in operculum and abopercular end can be observed using scanning electron microscopy. eggs recorded very often in stool of laotians belong mostly to opisthorchis viverrini and sporadically to metagonimus yokogawai.	1990	2337251
abscisic acid alters the metabolism of indole-3-acetic acid in senescing flowers of cucumis melo l.	experiments were conducted to investigate indole-3-acetic acid (iaa) and abscisic acid (aba) metabolism associated with postanthesis senescence of ovaries from nonpollinated muskmelon (cucumis melo l.) flowers. flowers attached to the vine were allowed to senesce for 4 days after anthesis or were harvested at full anthesis and aged for the same time interval with or without access to water. the iaa ester, amide-linked forms of iaa, free aba, and aba esters increased in senescent ovaries from flo ...	1990	16667865
plant regeneration from cotyledon protoplasts of xinjiang muskmelon.	cotyledon protoplasts were isolated from seedlings of xinjiang muskelon (cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified miller medium. high frequency division of the protoplast-derived cells was observed. agarose bead culture with b6s3 tobacco crown gall nurse cells was found most suitable for the cotyledon protoplasts when compared with other culture methods. intact plants were regenerated from the protocalli by a two-step culture procedure with liquid and ...	1990	24226702
water relations of seed development and germination in muskmelon (cucumis melo l.) : v. water relations of imbibition and germination.	the initiation of radicle growth during seed germination may be driven by solute accumulation and increased turgor pressure, by cell wall relaxation, or by weakening of tissues surrounding the embryo. to investigate these possibilities, imbibition kinetics, water contents, and water (psi) and solute (psi(s)) potentials of intact muskmelon (cucumis melo l.) seeds, decoated seeds (testa removed, but a thin perisperm/endosperm envelope remains around the embryo), and isolated cotyledons and embryon ...	1990	16667369
water relations of seed development and germination in muskmelon (cucumis melo l.) : iv. characteristics of the perisperm during seed development.	we previously reported that an apparent water potential disequilibrium is maintained late in muskmelon (cucumis melo l.) seed development between the embryo and the surrounding fruit tissue (mesocarp). to further investigate the basis of this phenomenon, the permeability characteristics of the tissues surrounding muskmelon embryos (the mucilaginous endocarp, the testa, a 2- to 4-cell-layered perisperm and a single cell layer of endosperm) were examined from 20 to 65 days after anthesis (daa). wa ...	1990	16667368
water relations of seed development and germination in muskmelon (cucumis melo l.) : iii. sensitivity of germination to water potential and abscisic acid during development.	muskmelon (cucumis melo l.) seeds are germinable 15 to 20 days before fruit maturity and are held at relatively high water content within the fruit, yet little precocious germination is observed. to investigate two possible factors preventing precocious germination, the inhibitory effects of abscisic acid and osmoticum on muskmelon seed germination were determined throughout development. seeds were harvested at 5-day intervals from 30 to 65 days after anthesis (daa) and incubated either fresh or ...	1990	16667367
sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (cucumis melo l.) fruits.	fruits of orange-fleshed and green-fleshed muskmelon (cucumis melo l.) were harvested at different times throughout development to evaluate changes in metabolism which lead to sucrose accumulation, and to determine the basis of differences in fruit sucrose accumulation among genotypes. concentrations of sucrose, raffinose saccharides, hexoses and starch, as well as activities of the sucrose metabolizing enzymes sucrose phosphate synthase (sps) (ec 2.4.1.14), sucrose synthase (ec 2.4.1.13), and a ...	1989	16667212
variability and correlations in muskmelon in relation to the cultivation method.	six fruit characters have been measured in 23 cultivars of cucumis melo, representing a wide geographical range. plants were grown both in the greenhouse and in the field. when the 23 cultivars were analyzed together, the largest component of variance was found between cultivars under both growth conditions, suggesting the existence of large genetic diversity for all the characters studied. generally, variance between plants within cultivars was less than or equal to variance between fruits with ...	1989	24227250
in vitro plant regeneration from leaf and cotyledon explants of cucumis melo l.	five different genotypes from in vitro as well as greenhouse grown melon plants were shown to be highly responsive for in vitro shoot formation from leaf explants when placed on basic ms medium supplemented with 1 mg/l 6-benzylaminopurine. in addition, a very suitable regeneration system was obtained when cotyledon pieces of mature seeds were incubated on the same culture medium. in this case, the first shoots already appeared after 10 days of incubation, and hundreds of shoots were formed on th ...	1989	24240446
blood sugar lowering potentiality of selected cucurbitaceae plants of indian origin.	using five experimental models, the blood sugar lowering efficacy of eight plants of cucurbitaceae family has been assessed. the ethanolic extract of cucumis sativus linn, cucumis melo utilissimum roxb, cucumis melo linn, benincasa hispida thunb cogn and tricosanthes anguina nees, when administered in 250 mg/kg dose, orally to rats failed to lower blood sugar or to depress the peak value, after glucose load. however, ethanolic extract of momordica charantia linn plant and coccinia indica whit an ...	1989	2620957
[characterization of xanthomonas campestris pv. cucurbitae, causal agent of the bacterial spot of squash].	bacterial leaf spot of squash was characterized for the first time in argentina. cultural, physiological, morphological and cross-infection tests on cucurbitaceae showed that the pathogen was xanthomonas campestris pv. cucurbitae (bryan) dye. the bacterium isolated from winter squash proved pathogenic for pumpkin, winter squash, cucumber and watermelon but no for muskmelon.	1989	2748850
post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.	two adjacent n-terminal tryptic peptides of the large subunit of ribulose bisphosphate carboxylase/oxygenase [3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39] from spinach, wheat, tobacco, and muskmelon were removed by limited tryptic proteolysis. characterization by peptide sequencing, amino acid composition, and tandem mass spectrometry revealed that the n-terminal residue from the large subunit of the enzyme from each plant species was acetylated proline. the sequence of the pen ...	1989	2928307
influence of glomus intraradices and soil phosphorus on meloidogyne incognita infecting cucumis melo.	the interaction among glomus intraradices, meloidogyne incognita, and cantaloupe was studied at three soil phosphorus (p) levels in a greenhouse. all plants grew poorly in soil not amended with p, regardless of mycorrhizal or nematode status. in soil amended with 50 mug p /g soil, m. incognita suppressed the growth of nonmycorrhizal plants by 84%. in contrast, growth of mycorrhizal plants inoculated with m. incognita was retarded by only 21%. a similar trend occurred in plants grown in soil with ...	1989	19287577
a simple purification of indole-3-acetic acid and abscisic acid for gc-sim-ms analysis by microfiltration of aqueous samples through nylon.	a simple procedure was developed for the partial purification of plant tissue samples to be analyzed simultaneously for indole-3-acetic acid (iaa) and abscisic acid (aba). the procedure relies on removal of contaminants by filtration through nylon and partitioning into dichloromethane. this procedure successfully purified both iaa and aba from muskmelon, cotton, and broccoli tissue. twenty individual samples can be purified and methylated in 8 h for analysis of free iaa and aba with gas chromato ...	1989	16666735
effects of air quality on foliar injury, growth, yield, and quality of muskmelon.	an evaluation of the effects of ambient ozone (o3) on muskmelon was conducted with the use of open-top chambers (otcs). 'superstar' muskmelons grown in charcoal-filtered (cf) chambers compared to those grown in nonfiltered (nf) chambers showed significant differences in the severity of visible foliar o3 injury. furthermore, plants grown in nf conditions had significantly less (21.3%) marketable fruit weight and fewer (20.9%) marketable fruit number than those from cf chambers. no differences wer ...	1988	15092549
effect of temperature on infection and survival of rotylenchulus reniformis.	from infestation of lettuce with preinfective females to egg deposition, populations of rotylenchulus reniformis from baton rouge, louisiana; lubbock and weslaco, texas; and mayaguez, puerto rico, required 41, 13, 7, and 7 days at 15, 20, 25, and 34 c, respectively. no nematode infection occurred at 10 c with any r. reniformis population, and the population from puerto rico did not reproduce at 15 c. nematode survival was not influenced by temperature, since populations from texas and louisiana ...	1988	19290223
regeneration of plants from leaf explants of cucumis melo cv. pusa sharbati.	leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of cucumis melo were cultured on a ms medium supplemented with a range and combination of growth regulators. maximum shoot differentiation from the leaf explants occurred in the combined presence of bap and 2ip at equimolar concentration of 1 μm. regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. for elongation the shoots were transferred to ms+bap [1 μm] ...	1988	24240267
nutrient salts promote light-induced degradation of indole-3-acetic acid in tissue culture media.	the disappearance of indole-3-acetic acid (iaa) from cell-free liquid culture medium was followed in response to nutrient salts found in murashige-skoog salt base, light, and ph range of 4 to 7. the loss of iaa was accelerated by light or murashige-skoog salts. however, the combination of both light and murashige-skoog salts acted synergistically to catalyze the destruction of over 80% of the original iaa within 7 days of continuous incubation. under these same conditions, the loss of iaa was de ...	1988	16666312
water relations of seed development and germination in muskmelon (cucumis melo l.) : i. water relations of seed and fruit development.	total water potential (psi), solute potential, and turgor potential of field-grown muskmelon (cucumis melo l.) fruit tissue (pericarp) and seeds were determined by thermocouple psychrometry at 5-day intervals from 10 to 65 days after anthesis (daa). fruit maturity occurred between 44 and 49 daa, and seed germination ability developed between 35 and 45 daa. pericarp psi was essentially constant at approximately -0.75 megapascal (mpa) from 10 to 25 daa, then decreased to a minimum value of -1.89 m ...	1988	16665921
pathway of assimilate transfer between mesophyll cells and minor veins in leaves of cucumis melo l.	photoassimilating mature leaves of cucumis melo exported carbon at a rate of 1.7 mg c·dm(-2)·h(-1). radiolabeling with (14)c showed that stachyose and raffinose are the main carbohydrates translocated. autoradiograms indicated that sieve elements of the abaxial phloem of minor veins are the sole conduits for carbon export from mature leaves and carbon import into immature leaflets. sieve elements of the abaxial phloem are associated with intermediary cells which are intimately connected with the ...	1987	24227267
sucrose metabolism in netted muskmelon fruit during development.	sugar content and composition are major criteria used in judging the quality of netted muskmelon (cucumis melo l. var reticulatus) fruit. sugar composition and four enzymes of sucrose metabolism were determined in ;magnum 45' muskmelon fruit at 10-day intervals beginning 10 days after pollination (dap) until full-slip (35 dap). sugar content increased in both outer (green) mesocarp and inner (orange) mesocarp between 20 and 30 dap. the major proportion of total increase in sugar was attributed t ...	1987	16665448
the effects of fruit juices and fruits on the absorption of iron from a rice meal.	the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...	1987	3593665
watermelon and ragweed share allergens.	a biotin-avidin amplified elisa was used to measure antigen-specific ige for ragweed, representative members of the gourd family (watermelon, cantaloupe, honeydew melon, zucchini, and cucumber), and banana in the sera of 192 allergic patients, each with an ige greater than or equal to 180 microns/ml. sixty-three percent (120/192) of the sera contained antiragweed ige, and of these patients, 28% to 50% contained ige specific for any single gourd family member. in contrast, no greater than 11% of ...	1987	3584742
are sucrosyl-oligosaccharides synthesized in mesophyll protoplasts of mature leaves of cucumis melo?	biosynthesis of sucrosyl-oligosaccharides (raffinose, stachyose) was traced in source leaves of cucumis melo after (14)c-photoassimilation. the main carbon compound exported was (14)c-labeled stachyose. no oligosaccharide synthesis was detected in young, importing leaves. mesophyll protoplasts, isolated from mature leaves which had previously photosynthesized (14)co2, did not contain (14)c-oligosaccharides but contained [(14)c]-sucrose and (14)c-hexoses. isolated minor-vein-enriched fractions fr ...	1986	24232761
the cucumis plastome: physical map, intrageneric variation and phylogenetic relationships.	a restriction map of the cucumis melo l. (melon) plastome was constructed by using several mapping approaches: single and double digestions of the chloroplast dna (chldna) with endonucleases (xhoi, smai, saci and pvuii) and hybridization to heterologous chldna probes and to isolated melon chldna fragments. four plastome-coded genes were located using heterologous probes. the overall organization and gene position of the melon plastome was found to be similar to that of tobacco and other angiospe ...	1985	24247447
promotion by ethylene of the capability to convert 1-aminocyclopropane-1-carboxylic acid to ethylene in preclimacteric tomato and cantaloupe fruits.	the intact fruits of preclimacteric tomato (lycopersicon esculentum mill) or cantaloupe (cucumis melo l.) produced very little ethylene and had low capability of converting 1-aminocyclopropane-1-carboxylic acid (acc) to ethylene. when these unripe tomato or cantaloupe fruits were treated with ethylene for 16 hours there was no increase in acc content or in ethylene production rate, but the tissue's capability to convert acc to ethylene increased markedly. such an effect was also observed in frui ...	1985	16664067
identification of platelet inhibitor present in the melon (cucurbitacea cucumis melo).	an active fraction was isolated from an aqueous melon extract (cucurbitacea cucumis melo) and was shown that it inhibits human platelet aggregation induced by epinephrine, adp, collagen, thrombin, sodium arachidonate, prostaglandin endoperoxide analogue u-46619 and paf-acether. identification of the active substance as adenosine was indicated by tlc which gave identical rf value compared to adenosine, by the uv spectrum, because the inhibitory effect on platelet aggregation disappeared after the ...	1985	3931281
mitochondrial gene expression in cucurbitaceae: conserved and variable features.	we have examined mitochondrial dna (mtdna) sequence conservation, transcriptional patterns of mitochondrial genes, and mitochondrial translation products in four species of the plant family cucurbitaceae, in which there is a seven-fold range in mitochondrial genome size. a set of conserved mtdna sequences which we term "core" dna is present in all cucurbit genomes examined. in watermelon, only those mtdna restriction fragments which contain "core" dna hybridize with mtrna. similar numbers of pol ...	1985	3870928
comparative morphology of eggs of heterophyids and clonorchis sinensis causing human infections in korea.	in order to provide some clues for differential diagnosis of trematode infections in fecal examination, the comparative morphology of eggs of 5 kinds of heterophyid flukes (metagonimus yokogawai, heterophyes heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus and pygidiopsis summa) and clonorchis sinensis was studied. the eggs were obtained from distal portion of uteri of worms which were recovered from men after treatment. the characteristic shape and apperance of each kind of ...	1984	12891010
cell surfaces in plant-microorganism interactions : iv. fungal glycopeptides which elicit the synthesis of ethylene in plants.	the production of ethylene by melon (cucumis melo cv cantaloup charentais) tissues is stimulated during incubation in the presence of fungal glycopeptides extracted from colletotrichum lagenarium, a pathogen of melon. these glycopeptides, called elicitors of ethylene, are found in the mycelium, the cell wall, and the culture filtrate. elicitation of ethylene is a relatively early phenomenon and lasts for several hours. upon purification of the crude elicitor extract by gel filtration and ion exc ...	1984	16663747
stereospecific conversion of 1-aminocyclopropanecarboxylic acid to ethylene by plant tissues : conversion of stereoisomers of 1-amino-2-ethylcyclopropanecarboxylic acid to 1-butene.	inasmuch as the molecule of 1-aminocyclopropanecarboxylic acid (acc) possesses reflective symmetry but lacks rotational symmetry, the two chemically alike methylene groups can be distinguished by a stereospecific enzyme. to determine whether acc conversion to ethylene by plant tissues proceeds in a stereospecific fashion, the four stereoisomers of 1-amino-2-ethylcyclopropanecarboxylic acid (aec) were administered to postclimacteric apple (malus sylvestris mill., var. golden delicious), excised p ...	1982	16662444
enhancement of wound-induced ethylene synthesis by ethylene in preclimacteric cantaloupe.	although intact fruits of unripe cantaloupe (cucumis melo l.) produce very little ethylene, a massive increase in ethylene production occurred in response to excision. the evidence indicates that this wound ethylene is produced from methionine via 1-aminocyclopropanecarboxylic acid (acc) as in ripening fruits. excision induced an increase in both acc synthase and the enzyme converting acc to ethylene. ethylene further increased the activity of the enzyme system converting acc to ethylene. the in ...	1982	16662200
organization of highly repetitive satellite dna of two cucurbitaceae species (cucumis melo and cucumis sativus).	the prominent satellites of the cucurbitaceae cucumis melo (melon) and cucumis sativus (cucumber) have been characterized, in actinomycin/cscl gradients where the satellite sequences can be separated from ribosomal, organelle, and main band dna the location of the satellites is different indicating a different gc content. the purified satellite of c. melo is cut by hindiii into a repeat unit of 380 bp; alui digestion gives rise to two bands (about 80 and 220 bp in size). the hindiii repeat unit ...	1982	6278425
divergence, differential methylation and interspersion of melon satellite dna sequences.	melon (cucumis melo) satellite dna consists of two components, q and s, each with a buoyant density in cscl of 1.707 g/ml, but differing by 9 degrees c in "melting" temperature. these physical properties appear to be in contradiction, since both depend on g + c content. in order to resolve this anomaly, base compositions were directly determined for isolated fractions. the low-"melting" component s contains 41.8% g + c, with 6% of c present as 5-methylcytosine, whereas q dna contains 54% g + c, ...	1981	6172117
the mitochondrial genome is large and variable in a family of plants (cucurbitaceae).	the genome sizes of mitochondrial dna from darkgrown (etiolated) shoots of several higher plants were determined by reassociation kinetics and restriction analysis. kinetic complexities obtained from reassociation kinetics measured spectrophotometrically indicate a mitochondrial genome size of 1600 md for muskmelon, 1000 md for cucumber, 560 md for zucchini squash and 220 md for watermelon (four species in the cucurbit family), as well as 240 md for pea and 320 md for corn. the kinetic curves al ...	1981	6269758
variations in the satellite dna content of cucumis melo in relation to dedifferentiation and hormone concentration.	total dna from cucumis melo contains a 1.706 satellite dna which can be resolved into two components; one of these components has a higher temperature of melting (component i) then the other component ii). in this study, we have further investigated these components by thermal denaturation and by eco r1 digestion. component i reveals a homogeneous melting profile and is only partially cleaved by eco r1, whereas component ii reveals a heterogeneous melting profile and is entirely digested by eco ...	1980	6253893
a lectin from the exudate of the fruit of the vegetable marrow (cucurbita pepo) that has a specificity for beta-1,4-linked n-acetylglucosamine oligosaccharides.	lectins are present in the exudate (presumably from the phloem) of the fruits of three species of the cucurbitaceae, namely vegetable marrow (cucurbita pepo), melon (cucumis melo) and cucumber (cucumis sativus). they are all strongly inhibited in their activities by chitin oligosaccharides, but only weakly by n-acetylglucosamine. glycopeptides from soya-bean agglutinin and fetuin are also strong inhibitors of cucurbita pepo lectin, indicating that it interacts with internal n-acetylglucosamine r ...	1979	534476
cell surfaces in plant-microorganism interactions: ii. evidence for the accumulation of hydroxyproline-rich glycoproteins in the cell wall of diseased plants as a defense mechanism.	enrichment of the cell wall in hydroxyproline-rich glycoprotein is involved in the defense of muskmelon (cucumis melo) seedlings to colletotrichum lagenarium, the causative agent of anthracnose. the extent to which this accumulation proceeds may be experimentally modified by treating plants with ethylene or growing them in the presence of free l-trans-hydroxyproline. it appears that the increase in the wall hydroxyproline-rich glycoprotein mediated through ethylene is paralleled by an increasing ...	1979	16660957
cell surfaces in plant-microorganism interactions: i. a structural investigation of cell wall hydroxyproline-rich glycoproteins which accumulate in fungus-infected plants.	infection of muskmelon cucumis melo seedlings by the fungus colletotrichum lagenarium causes a 10-fold increase in the amount of cell wall hydroxyproline-rich glycoprotein. evidence for this increase was provided by studying two specific markers of this glycoprotein, namely hydroxyproline and glycosylated serine. the lability of the o-glycosidic linkage of wall-bound glycosylated serine in the presence of hydrazine, was used to determine the amount of serine which is glycosylated.a large increas ...	1979	16660956
phytoparasitic nematodes adjacent to established strawberry plantations.	plant-nematode populations associated with uncultivated vegetation, adjacent strawberry plants, and alternate crop sites were studied at three locations in minnesota. at one site (forest lake), paratylenchus projectus, meloidogyne hapla, and pratylenchus tenuis were frequently associated with the roots of native vegetation. these nematode species were also present in adjacent strawberry beds. among alternate crops observed, oats and muskmelon usually supported the fewest nematodes although moder ...	1978	19305841
distinction between nuclear satellite dnas and chloroplast dna in higher plants.	triton x-100 solubilized chloroplast dna but not nuclear dna from a mixture of chloroplasts and nuclei. the buoyant density of chloroplast dna was different from that of the satellite dna in all of the species examined (phaseolus coccineus, cucumis sativus, cucumis melo, antirrhinum majus, vicia faba, oenothera fruiticosa youngii). chloroplast dna constituted between 4.3% and 0.25% of the total leaf dna in these species, and was present as 5 to 20 copies in each chloroplast.	1978	16660649
occurrence, isolation & characterisation of urease-inhibitor from melon (cucumis melo) seeds.		1978	748169
light-dependent emission of hydrogen sulfide from plants.	with the aid of a sulfur-specific flame photometric detector, an emission of volatile sulfur was detected from leaves of cucumber (cucumis sativus l.), squash and pumpkin (cucurbita pepo l.), cantaloupe (cucumis melo l.), corn (zea mays l.), soybean (glycine max [l.] merr.) and cotton (gossypium hirsutum l.). the emission was studied in detail in squash and pumpkin. it occurred following treatment of the roots of plants with sulfate and was markedly higher from either detached leaves treated via ...	1978	16660257
modifications induced by benomyl and related compounds into chloroplasts spectral patterns, photosynthetic rates and chlorophyll contents of spinacia oleracea and cucumis melo.		1977	861396
sequence arrangement in satellite dna from the muskmelon.	two fractions of a satellite dna from the muskmelon (cucumis melo l.) isolated as a unimodal peak from cscl gradients, differ in melting properties and complexity as estimated by reassociation kinetics. at 49.8 c, all of the low melting fraction was denatured and all of the high melting fraction was native. there were almost no partially denatured molecules detected in the electron microscope at this temperature. this observation provides direct evidence that the two fractions are not closely li ...	1977	16659902
occurrence of patulin in fruit and vegetables.	in different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ...	1977	613910
[patulin in foods of vegetable origin. 2. several kinds of fruit and vegetables and fruit and vegetable products (author's transl)].	patulin was found in fruit with spontaneous brown rot (bananas, pineapples, grapes, peaches, apricots) as well as in moldy compots and in sallow-thorn juice. fruit, vegetables and fruit and vegetable product were artificially infected with penicillium expansum, p. urticae and byssochlamys nivea; patulin was subsequently found in peaches, apricots, greengages, bananas, strawberries, honeydew melons, tomatoes, red and green paprika, cucumbers and carrots; in several kinds of compot, in tomato juic ...	1977	857490
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.	proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...	1976	24424985
a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing.	proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ...	1976	24424601
isolation and characterization of a proteinase from the sarcocarp of melon fruit.	a proteinase from the sarcocarp of melon (cucumis melo l. var. prince) was purified by a three-step procedure involving batch-wise treatment with cm-cellulose fibers, column chromatography on cm-cellulose powder and gel filtration on sephadex g-75. the final enzyme preparation was homogeneous on acrylamide gel electrophoresis. its molecular weight was estimated by two different methods to be about 50,000. anlayses indicated tha presence of 475 amino acid residues and at least 7 moles of hexose. ...	1975	5423
the complexity of satellite deoxyribonucleic acid in a higher plant.	purified satellite dna from melon (cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite dna, and one with 6000 times greater complexity. both components renatured very accurately, indicating a minimum of sequence divergence. centrifugation of the purified satellite dna in ag+/cs2so4 gradients resolved two major and ...	1975	1191264
pathogenicity and histopathology of rotylenchulus reniformis infecting cantaloup.	rotylenchulus reniformis was pathogenic to cantaloup (cucumis melo 'perlita') under greenhouse conditions. these findings confirm field symptoms of cantaloup infected with r. reniformis. histopathological studies show that the nematode penetrates the cortex perpendicular to the vascular system and comes to rest with the head against the endodermis in young roots. feeding stimulated the pericycle to either side of the endodermal feeding cell and caused cell hypertrophy with enlargement of the nuc ...	1975	19308147
novel properties of satellite dna from muskmelon.	the purified dense satellite of muskmelon (cucumis melo l.) represents 30% of the total dna and forms a sharp, unimodal peak in both neutral and alkaline cscl gradients. spectrophotometric melting and dna reassociation analyses revealed that one-third of the satellite is high melting and has a complexity of about 2.5 x 10(5) daltons, while the remaining two-thirds of the satellite melts 8 degrees lower and has a complexity of about 5 x 10(8) daltons. the thermal stability of reassociated satelli ...	1974	4524654
diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves.	expanding leaves of capsicum frutescens l. cv. california wonder, cucumis melo l. cv. hales best, and citrus sinensis l. osbeck cv. washington navel showed a marked diurnal periodicity in the incorporation of (14)c from photosynthetically fixed (14)co(2) into amino acids. incorporation was virtually nil at the beginning of the photoperiod, reached a maximum in the 6th to 7th hour and decreased during the latter part of the photoperiod. in capsicum frutescens this was apparently a reflection of t ...	1973	16658402
isolation and identification of the gibberellins of cucumis sativus and cucumis melo.	thin-layer chromatography, gas-liquid chromatography, and mass spectrometry were used to identify gibberellins isolated from mature seeds of both cucumis sativus (cucumber) and cucumis melo (muskmelon). the gibberellins were extracted and purified by organic solvent fractionation, paper and thin-layer chromatography, and crystallization. seeds of c. sativus were found to contain gibberellins a1, a3, a4, and a7 with a1 the predominant species. seeds of c. melo contained gibberellins a1 and a3 and ...	1972	24481557
ethylene: a natural regulator of sex expression of cucumis melo l.	sex expression in cucumber (cucumis sativus l.) and muskmelon (c. melo l.) was correlated with endogenous ethylene production. plants of gynoecious (all female) sex types of the two species produced more ethylene than monoecius (male-female) plants. c. melo plants of a gynoecious sex type that normally produce only pistillate (female) flowers, when grown with hypobaric ventilation to facilitate removal of endogenous gases by diffusion, produced perfect (hermaphroditic) flowers. when either the p ...	1972	16591971
pharmacodynamic investigations into the diuretic activity of cucumis melo seed (ether extract).		1970	5506675
control of fusarium wilt of muskmelon and other effects of benomyl soil drenches.		1970	5471339