Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| purification and properties of acetate kinase from acholeplasma laidlawii. | acetate kinase (ec 2.7.2.1) was purified from acholeplasma laidlawii cytoplasm by a combination of ammonium sulfate fractionation, gel filtration, diethylaminoethyl-cellulose chromatography, and affinity chromatography on 8-(6-aminohexylamino)-adenosine 5'-triphosphate conjugated to sepharose 4b. the enzyme was composed of polypeptide chains of about 50,000 molecular weight as estimated from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. under nondenaturating conditions, apparent mol ... | 1979 | 217865 |
| the ayerst award lecture 1978. organization and dynamics of membrane lipids as determined by magnetic resonance spectroscopy. | 1979 | 218704 | |
| activities of enzymes of purine and pyrimidine metabolism in nine mycoplasma species. | 1979 | 232630 | |
| physical characterization of the superhelical dna genome of an enveloped mycoplasmavirus. | mycoplasmavirus mvl2 is a nonlytic enveloped virion containing dna. this dna has been shown to be a double-stranded circular superhelical molecule of 11.8 kilobase pairs (7.8 x 10(6) daltons). the superhelix density is greater than that of phi x174 rfi but less than that of pm2 phage dna. a physical map of the mvl2 genome has been obtained using restriction endonucleases. | 1979 | 430595 |
| characterization of mycoplasmavirus mvl2 dna. | the size and molecular configuration of mycoplasmavirus mvl2 dna are presented. the dna was shown to be a covalently closed circular duplex molecule with a molecular weight of 7.4 x 10(6). in sucrose gradients at neutral ph, the form i and form ii dna molecules have sedimentation values of approximately 29s and 23s, respectively. under alkaline conditions, the form i and form ii have s values of 70 and 20, respectively. | 1979 | 430596 |
| studies on classification of acholeplasmas isolated from horses, cattle and abattoir sewage. | 1979 | 430937 | |
| the properties of gel state lipid in membranes of acholeplasma laidlawii as observed by 2h nmr. | 1979 | 437110 | |
| is the vertical disposition of mycoplasma membrane proteins affected by membrane fluidity? | the influence of the physical state of the membrane lipid matrix on the vertical disposition of membrane proteins was studied with acholeplasma laidlawii. changes in membrane fluidity were brought about by altering the fatty acid composition of membrane lipids, by changing the growth temperature, by aging of cultures and by inducing changes in the membrane lipid-to-protein ratio through treatment with chloramphenicol. the lactoperoxidase-mediated iodination technique was used to label membrane p ... | 1979 | 444513 |
| role of lipid fatty acyl composition and membrane fluidity in the resistance of acholeplasma laidlawii to complement-mediated killing. | 1979 | 448140 | |
| [lipid regulation of carbohydrate transport system in mycoplasma]. | cultivation of acholeplasma laidlawii cells in media containing unsaturated fatty acids results in changes of the physiological state of the membrane lipid bilayer due to preferable incorporation of an unsaturated fatty acid into lipids. the lipids are capable to regulate the transport activity since the transport rates for glucose, 3-o-methyl-c-glucose, glucerol and erythritol change considerably when the cells are cultivated in media containing different unsaturated fatty acids. the transport ... | 1979 | 454709 |
| the use of the anti-acholeplasma activities of streptomyces culture filtrates for the screening of new antibiotics. | a screening method for new antimicrobial agents from streptomyces culture filtrates was developed. three different types of test organisms were used for determining the antimicrobial activities of the culture filtrates: they were (a) an antibacterial activity test against staphylococcus aureus, (b) an antimycoplasmal activity test against acholeplasma laidlawii, and (c) a cytotoxicity test against hela-s3 cells. the active filtrates showing antimicrobial spectra (anticellogram) which did not mat ... | 1979 | 462473 |
| on the interaction between intrinsic proteins and phosphatidylglycerol in the membrane of acholeplasma laidlawii. | 1979 | 464610 | |
| replication of mycoplasmavirus mvl51: vi. acriflavine stimulates growth of this single-stranded dna virus. | 1979 | 486185 | |
| lipid distribution in acholeplasma laidlawii membrane. a study using the lactoperoxidase-mediated iodination. | the lactoperoxidase-mediated radioiodination has been applied to study the transbilayer distribution of phospho- and glycolipids in acholeplasma laidlawii membranes. after radioiodination, about 5% of the 125i-iodine was found in membrane lipids. a comparison of the labeling intensities of the various lipid species between iodinated intact cells and isolated membranes revealed that the glycolipids monoglucosyldiglyceride and diglucosyldiglyceride are located almost exclusively in the outer half ... | 1979 | 486468 |
| prevalence of mycoplasmal bovine mastitis in california. | seven species of mycoplasma plus one or more unknown species were found to cause bovine mastitis in california. both the frequency of cases and number of species of mycoplasma in samples received at the laboratory have increased from 1976 to 1978. by survey, nearly 4% of samples of bulk tank milk from dairy farms were found to contain mycoplasma of potential pathogenic significance. acholeplasma laidlawii was frequently isolated from samples both from cows and from farm bulk tanks during wet, ra ... | 1979 | 507492 |
| unusual positional distribution of fatty acids in phosphatidylglycerol of sterol-requiring mycoplasmas. | 1979 | 510549 | |
| composition and molecular organization of lipids and proteins in the envelope of mycoplasmavirus mvl2. | mvl2 virus was purified from culture supernatants of infected acholeplasma laidlawii cells by differential centrifugation, followed by velocity centrifugation in sucrose gradients. the purified virus contained 0.08 to 0.1 mumol of lipid phosphorous per ml of viral protein. thin-layer chromatography of viral lipids revealed the presence of phospho-, glyco-, and phosphoglycolipids identical with those found in the host cell membrane, but the relative amount of phosphatidylglycerol was much lower t ... | 1979 | 513201 |
| comparison of mycoplasmatales virus mv-lg-ps2-l172 with plasmavirus mv-l2 and the other mycoplasma viruses. | mycoplasma virus mv-lg-ps2-l172 was sensitive to heat (56 degrees c/30 minutes), nonidet-p40 and ether. in these respects it resembled plasmavirus mb-l2. however, it differed from mv-l2 (and the other mycoplasma viruses, mv-l1, mv-l3 and bn1 virus) in reciprocal plaque inhibition and serum neutralization tests (mv-l2 only). by plaque formation on host lawns resistant to the different mycoplasma viruses, including mv-lg-ps2-l172, this latter virus was shown to be distinct from the other viruses, ... | 1979 | 518304 |
| studies on bacterial cell wall inhibitors. vi. screening method for the specific inhibitors of peptidoglycan synthesis. | a screening method was established for selecting new specific inhibitors of bacterial cell wall peptidoglycan synthesis. in the primary test, culture broths of soil isolates were selected based on relative microbial activity. a culture, to be retained, must be active against bacillus subtilis and lack activities against acholeplasma laidawii. in the secondary test, inhibitors of bacterial cell wall synthesis were identified by their ability to prevent the incorporation of meso-[3h]diaminopimelic ... | 1979 | 528376 |
| carotenoids acts as reinforcers of the acholeplasma laidlawii lipid bilayer. | acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. the amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 m propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate incorporated into the membranes. the fatty acid composition of the propionate-grown cells differed from that of cells grown without propionate by containing odd-numbered rather than e ... | 1979 | 533770 |
| scanning electron microscopy of acholeplasma colonies on agar. | the colony formation and morphology of acholeplasma laidlawii and of an acholeplasma species was studied by scanning electron microscopy. in the colonies of both acholeplasma spp. large irregularly shaped cells, spherical cells, chains of beads, and long filaments with small bulbous distensions were seen. the membrane of some of the large cells seemed to be perforated, giving the cell a pitted appearance. | 1979 | 534959 |
| structural and biological properties of mycoplasmavirus mvl3: an unusual virus-procaryote interaction. | the kinetics of adsorption and growth of mycoplasmavirus mvl3 in acholeplasma laidlawii 1305/68 host cells have been studied with one-step growth, premature lysis, and single-burst experiments. the virus was found to kill infected host cells. virus release starts 90 min after infection and continues for about 10 to 15 h. hence, virus production is unlike the classical lytic bacteriophages and instead resembles nonlytic cytocidal animal viruses. structural details of the virus are described, and ... | 1979 | 541825 |
| binding of lectins to membranes of mycoplasmas from aging cultures. | the binding of iodinated concanavalin a (con a) and ricinus communis agglutinin (rca) to intact cells and isolated membranes of acholeplasma laidlawii, mycoplasma hominis and mycoplasma capricolum decreased with the progression of the culture from the mid- to the late-logarithmic phase of growth. the binding of the lectins to acholeplasma laidlawii membranes had no significant effect on membrane fluidity, as assessed by electron-paramagnetic resonance spectroscopy of spin-labelled fatty acids, a ... | 1979 | 542135 |
| [studies of mycoplasma mastitis in cattle. 5. studies of udder pathogenicity of mycoplasma and acholeplasma strains of different origins]. | isolated acholeplasma laidlawii strains exhibited highly differentiated behaviours regarding their udder pathogenicity. twelve of 16 tested strains were pathogenic to udder. symptoms of acute udder inflammation were caused by all ten a. laidlawii strains isolated from differentiated material of calf, but by only two of six strains isolated from differentiated material of cattle. intracisternal instillation of both strains from milk and one strain each from udder skin or cervical mucus caused mer ... | 1979 | 547917 |
| [studies of mycoplasma mastitis in cattle. 6. testing of mycoplasma isolated from milk for udder pathogenicity]. | strains of m. bovis, a. laidlawii, a. axanthum, and one unidentified strain of the family of mycoplasmataceae as well as altered secretion obtained from cows with mastitis were intracisternally applied in experiments and proved to be pathogenic to cattle udder. the results are likely to suggest the importance of mycoplasma isolated from the milk of mastitis cows to the aetiology of enzootic mastitis in three large dairy cattle stocks. intravenous application of m. bovis and a. laidlawii caused n ... | 1979 | 547918 |
| [studies of mycoplasma mastitis in cattle. 7. mycoplasma mastitis in 3 dairy cattle herds]. | reported in this paper is the occurrence of enzootic mastitis in three dairy cattle stocks. the outbreaks had been caused by mycoplasma bovis, acholeplasma laidlawii, acholeplasma axanthum as well as by one unidentified strain of the family of mycoplasmataceae. all animals with positive response to mycoplasma tests were identified an selected by repetitive testing of cultures in milk samples which had been taken from all lactating and dry cows and heifers as well as by evaluation of organ sample ... | 1979 | 547919 |
| [mycoplasma mastitis in cattle]. | reported in this paper are experiments from which the conclusion was drawn that acheloplasma laidlawii could cause changes in the mammary gland under certain circumstances. | 1979 | 551753 |
| [experience in identification and control of mycoplasma in dairy cattle stock under routine laboratory conditions]. | exacting demands have to be met by milk testing routine laboratories where mycoplasma species are to be cultured from milk samples. cleanliness of sampling, immediate cold storage, and no-delay transport of the milk samples to the testing centre are of greatest importance to the informative value of all mycoplasma tests. in the summer season, it is recommended to freeze the milk samples immediately after sampling. mycoplasma broth should not be inoculated to animals of the herd from which the sa ... | 1979 | 551756 |
| [acholeplasma laidlawii--a pathogenic or apathogenic species?]. | the question is discussed, whether acholeplasma laidlawii is a pathogenic or apathogenic species. studies have shown that it is a species of potential pathogenicity, however, with virulence properties differing greatly by strains. nothing so far has become known of contributive factors which might be needed for an infectious disease to develop. | 1979 | 551758 |
| simplified preparation of mycoplasmas, an acholeplasma, and a spiroplasma for scanning electron microscopy. | a simple, effective procedure was developed for scanning electron microscopic examination of mycoplasmas and similar organisms. cultivation of several mycoplasmal species, an acholeplasma, and a spiroplasma in broth media in leighton tubes with cover slips resulted in attachment of the organisms to the cover slips. the attached cells were easily processed for either scanning electron microscopy or light microscopy. by eliminating the need for centrifugation, which was used in previously describe ... | 1979 | 370106 |
| comparative studies between microbiological culture and uptake of uridine/uracil to detect mycoplasmal infection of cell cultures. | 1979 | 376320 | |
| alteration of colonial morphology of acholeplasma laidlawii and acholeplasma modicum by infection with mycoplasmatales viruses. | morphologically aberrant colonies resulted from the infection of acholeplasma laidlawii with two of its three known viruses and from acholeplasma modicum cells naturally carrying virus. the patterns of colonial alteration differed between cells infected with the two a. laidlawii viruses. colonies derived from single cells infected with the bullet-shaped virus mv-l1 (mycoplasmatales virus-laidlawii-1) had a radial sectoring pattern of intracolonial swellings ("blebs"), whereas cells infected with ... | 1979 | 378947 |
| [comparative study of rosette and plaque formation in rats infected with mycoplasma arthritidis and acholeplasma laidlawii]. | m. arthritidis suppresses rosette- and plaque-formation in rats infected with this strain of mycoplasma. on the 15th day the immune response was restored in the control and experimental groups of animals. in later stages a strong stimulation of rfc and pfc was recorded, which levelled on day 150. on the contrary, a. laidlawii stimulated rfc and pfc in all stages of infection. a relationship between the phenomena described and pathogenic properties of the mycoplasma is discussed. | 1979 | 380687 |
| [action of mycoplasma arthritidis and acholeplasma laidlawii on the immunological reactivity of balb/c mice infected with the rauscher leukemia virus]. | 1979 | 389300 | |
| determination of membrane properties at the molecular level by carbon-13 and deuterium magnetic resonance. | 1978 | 279301 | |
| bristol laboratories division, bristol-myers company, syracuse, n.y. | 1978 | 348086 | |
| acid-soluble nucleotides of acholeplasma (mycoplasma) laidlawii a. | a study was made of the acid-soluble nucleotides present in acholeplasma (mycoplasma) laidlawii a during growth. the nucleotide pool of this organism is meagre, both qualitatively and quantitatively. the main nucleotides are uridine mono- and diphosphates, and none of the more complex nucleotide compounds, such as uridine diphosphate (udp) sugars or sugar peptides, required for cell wall formation, were detected. the nucleotide composition of a. laidlawii a is compared with that of the streptoco ... | 1978 | 358744 |
| mechanisms of thermophily. | 1978 | 365460 | |
| acholeplasma laidlawii membranes: an electron spin resonance study of the influence on molecular order of fatty acid composition and cholesterol. | 1978 | 216314 | |
| alteration by cereolysin of the structure of cholesterol-containing membranes. | when erythrocyte membranes were treated with cereolysin, negatively stained and examined by electron microscopy, ring and arc-shaped structures were observed in the membrane. the outside diameter of the rings varied from 33 to 50 nm with a border thickness of 6.7 to 8.3 nm. the arcs varied in length from 33 to 170 nm with a border thickness of also 6.7 to 8.3 min. when right-side-out erythrocyte ghosts which had been treated with cereolysin were examined by electron microscopy after freeze-fract ... | 1978 | 414780 |
| endogenous hprt activity in mycoplasmas isolated from cell cultures. | five mycoplasma species most frequently isolated from cell cultures were tested for the presence of endogenous hypoxanthine phosphoribosyl-transferase (hprt) activity. all of the five, cultured in cell-free medium, contained variable but significant levels of hprt. two strains of m. hyorhinis exhibited a 13-fold difference in their specific hprt activity. when infected with any of these mycoplasma species, hprt-deficient mouse cell mutants rapidly acquired a cell-associated hprt activity; howeve ... | 1978 | 569123 |
| membrane lipid biosynthesis in acholeplasma laidlawii b: elongation of medium- and long-chain exogenous fatty acids in growing cells. | the chain elongation of a wide variety of exogenous fatty acids and the subsequent incorporation of the chain elongation products into the total membrane lipids of acholeplasma laidlawii b were systematically studied. within each chemical class of fatty acids examined, the extent of chain elongation increased with increases in chain length, reached a maximum value, and then declined with further increases in chain length. depending on chemical structure, exogenous fatty acids containing less tha ... | 1978 | 618849 |
| organometallic fatty acid and phospholipid analogs. synthesis and incorporation and detection in model membranes and biomembranes. | 1978 | 620019 | |
| inhibition of interferon induction in mice by mycoplasmas and mycoplasmal fractions. | 1978 | 622377 | |
| [transport system for carbohydrates using a model of membrane vesicles from acholeplasma laidlawii cells]. | 1978 | 627681 | |
| disposition of phosphatidylglycerol in metabolizing cells of acholeplasma laidlawii. | 1978 | 631331 | |
| the planar distributions of surface proteins and intramembrane particles in acholeplasma laidlawii are differentially affected by the physical state of membrane lipids. | we have studied the influence of changes in lipid organization on the planar distribution of two classes of membrane proteins: integral proteins which have amino groups exposed to labelling at the membrane surface by the biotinavidin-ferritin procedure, and those proteins which penetrate the lipid bilayer sufficiently to be seen as intramembranous particles by freeze-fracture electron-microscopy. when the membranes are examined at temperatures below the lipid phase transition, the first class is ... | 1978 | 638151 |
| membrane lipid metabolism in acholeplasma laidlawii a ef 22. influence of cholesterol and temperature shift-down on incorporation of fatty acids and synthesis of membrane lipid species. | 1. membrane lipid metabolism in acholeplasma laidlowii a ef 22 has been studied under different conditions by applying three different techniques for changing membrane viscosity: fatty acid and cholesterol supplementation and temperature changes. 2. the molar relationship between the two dominating membrane lipids, monoglucosyldiglyceride and diglucosyldiglyceride, is to a large extent determined by membrane viscosity properties. this is shown by the varying metabolic responses occurring during ... | 1978 | 639825 |
| growth and membrane lipid properties of acholeplasma laidlawii b lacking fatty acid heterogeneity. | 1978 | 643062 | |
| physico-chemical properties of phosphatidylglycerol in membranes of acholeplasma laidlawii. a study with phospholipase a2. | 1978 | 648516 | |
| sensitivites in vitro to antimicrobial drugs of bovine mycoplasmas isolated from respiratory and genital tracts. | a total of 155 mycoplasma strains were examined for sensitivity to nine antibiotics and four nitrofurans by the agar dilution method. they consisted of 69 strains of mycoplasma bovirhinis, 33 strains of m. bovigenitalium, 49 strains of acholeplasma laidlawii and four strains of a. modicum isolated from the nasal secretions, tracheas and lungs of calves manifesting respiratory symptoms and from bovine genital tracts collected at a slaughterhouse. as a result, furamizole and mitomycin c showed the ... | 1978 | 652043 |
| the positional distribution of a series of positional isomers of cis-octadecenoic acid in phosphatidylglycerol from acholeplasma laidlawii b. | 1978 | 656453 | |
| [energetics of active carbohydrate transport into mycoplasma cells]. | possible energy supply pathways of active transport of non-metabolized 3-o-methylglucose (3-o-mg) into acholeplasma laidlawii cells are studied. 3-o-mg transport is sensitive to arsenate, inhibitors of glucolysis and membrane mg-dependent atpase (dicyclohexylcarbodiimide) and to uncoupling agents. it is suggested that glycolysis is the main energy source, which main enzymes are found in cell-free extracts. a. laidlawii cells are capable to glycolytic atp synthesis using glucose as a substrate bu ... | 1978 | 656486 |
| efficiency and specificity of the sequential extraction of membrane proteins of acholeplasma laidlawii with the neutral detergent tween 20. | 1978 | 658403 | |
| replication of mycoplasmavirus mvl51. v. in vivo synthesis of virus-specific proteins. | 1978 | 664226 | |
| [lung tissue alterations following experimental infection of mice with acholeplasmae isolated from simians with hematosarcoma]. | this paper is devoted to the study of pathogenic acholeplasmae potencies. two strains of acholeplasmae isolated from the blood and organs of papio hamadryas suffering from hemoblastosis and a standard ach. laidlawii a. strain were subjected to the comparative study with the use of a "pulmonary model" in experiments on mice. morphological investigations showed affections of the pulmonary tissue characterized by stage-by-stage inflammatory-destructive changes analogous to the lesions found in "myc ... | 1978 | 665035 |
| lipid compositional manipulation in acholeplasma laidlawii b. effect of exogenous fatty acids on fatty acid composition and cell growth when endogenous fatty acid production is inhibited. | a variety of potential inhibitors of de novo fatty acid biosynthesis have been tested for activity in acholeplasma laidlawii b. two compounds, avidin and n,n-dimethyl-4-oxo-2trans-dodecenamide (cm-55), an antimicrobial fatty amide, strongly inhibit de novo biosynthesis without nonspecific toxic effects at moderate dosages. avidin is the more potent inhibitor, abolishing de novo fatty acid synthesis and greatly reducing the chain elongation of exogenous fatty acids at level of 25 u/l. cm-55 gives ... | 1978 | 667693 |
| induction of a ph-stable interferon in sheep lymphocytes by mycoplasmatales virus mvl2. | the data presented in this report show that the enveloped mycoplasmatales virus mvl2 is capable of interferon induction in sheep peripheral blood lymphocytes. the phenomenon was dose dependent, requiring approximately 10(8) plaque-forming units of virus per 2 x 10(6) lymphocytes. the interferon was stable to ph 2.0 treatment, was produced in moderately high levels (greater than 1,000 units), and met many of the criteria for classification as a type i interferon. heat-inactivated mlv2 lost its ab ... | 1978 | 669793 |
| isolation of mycoplasma arginini from swine and from a swine waste disposal system. | mycoplasma arginini was isolated from the pharynges and tonsils of 4 of 6 swine necropsied during an epizootic of respiratory tract diseases and lameness. the organism was also isolated from 2 of 16 asymptomatic swine examined in the same herd approximately 10 months after the epizootic. in addition, m arginini, acholeplasma laidlawii, and other acholeplasmas were isolated from recycled effluent of an anaerobic lagoon used for disposal of waste from the swine herd. this observation represents th ... | 1978 | 677536 |
| differences in incorporation of nucleic acid bases and nucleosides by various mycoplasma and acholeplasma species. | eight species representative of the serological diversity of the mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (eagle). for acholeplasma laidlawii, labeling occurred mainly during the first 4 h of incubation, with substrate saturation at 20 micron. all organisms tested incorporated uracil, adenine, and guanine; none incor ... | 1978 | 681280 |
| the distribution of molecular classes of phosphatidylglycerol in the membrane of acholeplasma laidlawii. | a double-label technique has been applied to study the distribution of different molecular classes of phosphatidylglycerol in the membrane of acholeplasma laidlawii. after growth on oleic acid, 16% of the total phosphatidylglycerol contains two oleic acid residues and 84% contains one oleic acid and one saturated fatty acid. the dioleoyl phosphatidylglycerol is present in equal amounts in the outer and the inner layer of the membrane. phosphatidylglycerol which is associated with membrane protei ... | 1978 | 687626 |
| a severe outbreak of bovine mastitis assoicated with mycoplasma bovigenitalium and acholeplasma laidlawii. | an outbreak of clinical mastitis is described in which 75 cows of a herd of 200 friesian cows were affected in one or more quarters over a period of three months. sixty animals failed to return to normal despite various intramammary and systemic antibiotic treatments. heifers, dry cows and lactating animals were affected. the milk of 15 out of 53 animals tested yielded mixed cultures of mycoplasma bovigenitalium and acholeplasma laidlawii. thirteen of 34 sera showed evidence of antibodies to a l ... | 1978 | 695253 |
| properties of a persistent viral infection: possible lysogeny by an enveloped nonlytic mycoplasmavirus. | mvl2, an enveloped double-stranded dna mycoplasmavirus, causes a nonlytic infection of acholeplasma laidlawii leading to the establishment of a persistent infection. persistently infected clones were found to be resistant to superinfection by homologous virus, but could be infected by heterologous virus. cells in a persistently infected culture had the potential to produce virus and transmitted this potential as a stable heritable trait. mitomycin c and uv light induced an increase in infectious ... | 1978 | 702650 |
| water binding and phase structures for different acholeplasma laidlawii membrane lipids studied by deuteron nuclear magnetic resonance and x-ray diffraction. | water binding capability and phase structures for different lipid species extracted from acholeplasma laidlawii a membranes have been studied using deuteron nuclear magnetic resonance and low-angle x-ray diffraction. the dominating membrane lipids are monoglucosyldiglyceride and diglucosyldiglyceride and each of them takes up limited amounts of water (bound plus trapped), i.e., up to 13% (w/w), whereas the phospholipids and phosphoglycolipids have larger hydration capacities. addition of magnesi ... | 1978 | 708724 |
| rapid spectrophotometric determination of acholeplasma laidlawii membranes. | the amount of acholeplasma laidlawii membranes can be accurately, rapidly, and simply determined by spectrophotometric measurements after solubilization in 0.02 m sds, 0.02 m tris-hcl (ph 8.0). extinction coefficients from the 260- to 300-nm-absorption region are adequate for quantitative membrane determination. the following values were found: e1%1cm,280 = 9.11 +/- 0.63, e1%,s1cm,280 = 8.52 +/- 0.40 (corrected for light scattering), e1%1cm,280--310 = 8.15 +/- 0.35. the last value is the most sa ... | 1978 | 709438 |
| crossed immunoelectrophoresis, in the presence of tween 20 or sodium deoxycholate, of purified membrane proteins from acholeplasma laidlawii. | five membrane proteins from acholeplasma laidlawii have been previously purified on a large scale. these proteins have been used to establish the relationship between the precipitation lines obtained by crossed immunoelectrophoresis of solubilized cell membrane proteins from a. laidlawii in the presence of the neutral detergent tween 20 or those obtained in the presence of the anionic detergent sodium deoxycholate. this relationship, which was unambiguously established for four of the five prote ... | 1978 | 711668 |
| disposition of membrane proteins as affected by changes in the electrochemical gradient across mycoplasma membranes. | 1978 | 718682 | |
| cholesterol uptake is dependent on membrane fluidity in mycoplasmas. | the transfer of elaidate-enriched acholeplasma laidlawii cells in culture from 37 degrees c to 4 degrees c virtually arrested exogenous cholesterol incorporation into the cell membrane. cholesterol uptake continued, though at a slower rate, in oleate-enriched a. laidlawii cells undergoing similar temperature shift-down. it is concluded that the incorporation of exogenous cholesterol into the cell membrane of living mycoplasmas is rapid when the membrane lipid bilayer is in the liquid-crystalline ... | 1978 | 718901 |
| effect of acriflavine on ultraviolet inactivation of acholeplasma laidlawii. | an increased sensitivity to inactivation was observed when ultraviolet light-irradiated acholeplasma laidlawii cells were plated on medium containing either acriflavine or chloramphenicol. chloramphenicol reduced liquid holding recovery (dark repair) to about 10% of that in untreated irradiated cells. in acriflavine treated cells no dark repair could be observed and there was a progressive degradation of cell dna during holding. while the primary effect of acriflavine may be to inhibit excision ... | 1978 | 718990 |
| photodynamic inactivation and its repair in mycoplasmas. | photodynamic inactivation is the loss in viability observed when organic dye-treated cells are exposed to visible light and molecular oxygen. the photodynamic inactivation of mycoplasmas, the smallest free living cells, has been studied. depending on the extent of inactivation in acholeplasma laidlawii, photodynamic induced damage can be repaired if the irradiated cells are incubated in the dark in buffer. analysis of the dna of these cells shows that photodynamic inactivation induces single str ... | 1978 | 728473 |
| [structural study of mycoplasma by a scanning electron microscopy method]. | scanning electron microscopy was applied to the study of mycoplasma arthritidis, myc. gallisepticum, and acholeplasma laidlawii. small spherical elements (0.3--0.5 micrometer) formed a reticular "framework" of the colonies in which larger (1--1.5 micrometer) spheroid or irregular-shaped cells (in case of myc. gallisepticum) and also rod-shaped (1--3 micrometer), filamentous (3--5 micrometer) forms and "large bodies" (5 x 3--8 x 3.5 micrometer) were located. apparently the latter represented cong ... | 1978 | 747025 |
| membrane enzymes: artifacts in arrhenius plots due to temperature dependence of substrate-binding affinity. | for the membrane sodium-stimulated magnesium-adenosinetriphosphatase of acholeplasma laidlawii b both the vmax and km values in the michaelis equation very strongly with temperature. simulations of arrhenius plots show that an enzyme with a temperature-dependent km can yield a variety of arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration. | 1978 | 146257 |
| role of cholesterol in the action of cereolysin on membranes. | 1978 | 102256 | |
| the nature and location of acholeplasma laidlawii membrane proteins investigated by two-dimensional gel electrophoresis. | the high resolution, two-dimensional electrophoresis system for the separation of proteins described by o'farrell, (o'farrell, p.h. (1975) j. biol. chem. 250, 4007--4021) has been modified for the separation of acholeplasma laidlawii proteins. reproducible protein patterns have been obtained from a. laidlawii cell, membrane and soluble protein preparations. the isoelectric focusing of membrane proteins was greatly improved by removing the bulk of the membrane lipid before solubilizing the protei ... | 1978 | 102346 |
| size and shape of mycoplasma virus mv-lg-l 172 dna. | sedimentation of dna of mycoplasma virus mv-lg-l 172 in neutral and alkaline density gradients showed that the chromosome of this virus is most probably formed by a double-stranded circular dna molecule with a molecular weight of approx. 10(7) daltons. these results were confirmed by direct electron microscopy of the viral dna. gamma-irradiation of the virus caused in its dna single breaks on the average per each 66 ev of absorbed energy. virus survival depended exponentially on the irradiation ... | 1978 | 35941 |
| fractionation of membrane proteins by hydrophobic interaction chromatography and by chromatography on agarose equilibrated with a water-alcohol mixture of low or high ph. | 1978 | 25906 | |
| symport h+/carbohydrate transport into acholeplasma laidlawii cells. | 1978 | 26600 | |
| effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum (t-strain mycoplasma). | the effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum type viii was determined by using a well-buffered broth medium containing 10 mm urea. the addition of nh4cl to the medium at concentrations up to 10 mm did not affect growth; however, addition of larger quantities progressively decreased both the specific growth rate (mu) and the maximum yield of the culture, with concentrations of 80 mm completely inhibiting growth. addition of either 150 mm kcl or ... | 1978 | 26668 |
| physiological role and membrane lipid modulation of the membrane-bound (mg2+, na+)-adenosine triphosphatase activity in acholeplasma laidlawii. | the membrane-bound adenosine triphosphatase (atpase) activity of acholeplasma laidlawii b differs in many respects from the common (mg2+, ca2+)-atpase activity of higher bacteria, most notably in that it is specifically activated by mg2+ and strongly and specifically stimulated by na+ (or li+). various inhibitors diminish the atpase activity with a concentration dependence which suggests that a single enzyme species is responsible for all of the observed atp hydrolytic activity (both basal and n ... | 1978 | 31351 |
| localization of enzymes in ureaplasma urealyticum (t-strain mycoplasma). | ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. the membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. these values are in large excess of those reported for classical mycoplasmas, suggesting that the ureaplasma membrane fraction was heavily contaminated with proteins derived from the growth medium. the u. urealyticum urease activity was localized in the cytoplasmic fraction, whereas the adenosine triphosphatase activity was local ... | 1977 | 15980 |
| ultrastructural and cytochemical studies on anionic surface sites of mycoplasma membranes. | negatively charged, chemical groups on the surfaces of mycoplasma membranes were studied in the electron microscope by staining with positively charged ferric oxide hydrosols in the acetic acid (ai-reagent) or propanoic acid (pi-reagent), respectively. the technique of thin sectioning was used. with the ai-reagent, no significant binding of the ferric oxide particles was observed on the mycoplasma membranes. with the lipophilic pi-reagent, the membrane surfaces of m. mycoides subsp. capri, m. my ... | 1977 | 65876 |
| effect of various culturing conditions of acholeplasma laidlawii on the yield and properties of the cells and cell membrane. | acholeplasma laidlawii strain b cell cultured in complex medium have an optimal temperature of growth at 31 degrees. maximal growth at this temperature is 80% higher than at 37 degrees. by stirring of the culture, the yield of the cell mass of 4 liter-cultures increases by 200%. with these modifications the yield of cell membranes increases 3--4 times with respect to that of static culturing at 37 degrees. membranes obtained from better aerated cultures have a lower flavin and carotenoid content ... | 1977 | 67754 |
| antibiotics for elimination of mycoplasmas and ureaplasma from bovine semen. | minocin at 500 micrograms/ml of semen extender eliminated ureaplasma from naturally or artificially infected bovine semen. minocin with lincospectin eliminated acholeplasma laidlawii, mycoplasma bovigenitalium, bovis, canadense, and arginini from artificially infected semen. stabilization times of 15 min at 35 c and 3 h at 4 c are important considerations to maximize antibiotic activity. | 1977 | 141466 |
| recognition of different pools of phosphatidylglycerol in intact cells and isolated membranes of acholeplasma laidlawii by phospholipase a2. | phospholipase a2 (ec 3.1.1.4) from pig pancreas hydrolyzes phosphatidylglycerol in intact cells and isolated membranes of acholeplasma laidlawii. complete degradation of phosphatidylglycerol in intact cells at 37 degrees c does not result in lysis as shown by the retention of intracellular k+ ions and the cytoplasmic glucose-6-phosphatase, as well as the inability to detect activity of membrane-bound intracellular nadh-oxidase. a. laidlawii was grown on linoleic acid. phospholipase a2 treatment ... | 1977 | 191065 |
| cholesterol transfer from serum lipoproteins to mycoplasma membranes. | 1977 | 199249 | |
| [experimental infection of dogs with myocplasmas (acholeplasma laidlawii)]. | 1977 | 202113 | |
| heterogeneity among strains of mycoplasmatales virus-laidlawii 2. | five isolates of mycoplasmatales virus-laidlawii 2 (mv-l2) derived from bovine strains of acholeplasma laidlawii were shown to differ in host range, plaque morphology and neutralization tests with mv-l2 antiserum. cross-testing using virus resistant clones of a. laidlawii confirmed the heterogeneity of this group. adaptation of viruses to sub-optimal host was demonstrated. | 1977 | 560426 |
| lymphocyte activation by various mycoplasma strains and species. | the capacity of various mycoplasma strains and species to induce lymphocyte transformation in vitro was studied. of six strains of mycoplasma pulmonis studied, five displayed mitogenic activity with rat lymphocytes. among those m. pulmonis strains, our mp15 isolate and the negroni strain exhibited particularly potent mitogenic capacity. the murine mycoplasmas m. neurolyticum and m. arthritidis shared this mitogenicity for rat lymphocytes. however, the human mycoplasmas m. fermentans, m. pneumoni ... | 1977 | 562852 |
| [experimental infection of mice, gerbils, and rats with mycoplasms from canine pericardium and cardial value (author's transl)]. | mycoplasms isolated from heart valves (a. laidlawii (a 42 hzbtl)) and pericardium (m. canis (a 56 hzkl)) of dogs were investigated for infectivity of rats, mice and gerbils (meriones unguiculatus). in mice the species m. spumans (pg 13), m. maculosum (pg 15), m. edwardii (pg 24) and m. molare (h 542) were investigated too. the mycoplasmas were inoculated by oral, subcutaneous, intravenous and intraperitoneal route. intraperitoneally the mycoplasmas were given without and with complete and incomp ... | 1977 | 595971 |
| ultrastructural localization of tellurite reduction in acholeplasma species. | aerobic reduction of tellurite by five acholeplasma species and two mycoplasma species was investigated by light and electron microscopy. among the acholeplasma species, colonies of a. laidlawii and a. oculi exhibited a heavy, macroscopically visible reduction of tellurite, whereas the reaction of a. axanthum was weaker. a. granularum and a. modicum did not reduce the substrate under the experimental conditions employed. the two subspecies of m. mycoides also reacted with tellurite, as did also ... | 1977 | 329638 |
| localization of a phosphoglycolipid in mycoplasma membranes using specific anti-lipid-antibodies. | a phosphoglycolipid, presumably identical to glyceryl-phosphoryldiglycosyl diglyceride, is the main component of the membrane glycolipids of mycoplasma mycoides subsp. capri. it is immunologically active. anti-phosphoglycolipid antibodies were induced in rabbits by intravenous injection of the flocculated complexes of methylated bovine serum albumin with a mixture of the phosphoglycolipid and the auxiliary lipids, phosphatidyl-choline and cholesterol. the specificities of the antibodies directed ... | 1977 | 337735 |
| growth of an enveloped mycoplasmavirus and establishment of a carrier state. | the growth of an enveloped dna-containing mycoplasmavirus (mvl2 obtained from r.n. gourlay) has studied, by using the indicator host acholeplasma laidlawii strain ja1. from virus one-step growth curves, artificial lysis experiments, and infected cell growth curves, it was found that virus infection is nonlytic. newly infected cells grow slower and are osmotically more stable than uninfected cells. however, 4 to 6 h after infection, the cells reach a carrier state in which cell growth rate and os ... | 1977 | 405508 |
| microbiological assay of thiopeptin in broiler rations. | 1977 | 833096 | |
| [bovine mastitis caused by acholeplasms laidlawii]. | 1977 | 836266 | |
| acholeplasma laidlawii infection by group 3 mycoplasmavirus. | 1977 | 841870 | |
| host cell and ultraviolet reactivation of ultraviolet-irradiated mycoplasmaviruses. | the mycoplasma acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (uv) reactivation of uv-irradiated mycoplasmaviruses. host cell reactivation was examined by comparing the survival abilities of uv-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untreated and on acriflavine-treated cells. acriflavine treatment inhibited cell exision repair. decreased survival on the acriflavine-treated cells demonstrated host cell reactivation. ... | 1977 | 845120 |
| [characterization of acholeplasma strains by horizontal polyacrylamide flat gel electrophoresis (author's transl)]. | proteins extracted with phenol-acetic acid-water (2:1:0.5, w/v/v) from acholeplasma laidlawii (pg 8), a. granularum (bts-39), a. oculi (19l), a. modicum (pg 49), a. axanthum (s743) and the acholeplasma strains c1 and c112 (which were isolated from aborted horse foetuses) were compared by electrophoresis in horizontal acidic polyacylamide flat gel using the electrophoresis equipment lkb multiphor 2117. in this system the gels are not prepared in the electrophoresis chamber but between glas plates ... | 1977 | 848216 |
| the removal of the neutral detergent tween 20 from solubilized membrane proteins of acholeplasma laidlawii and other proteins by agarose-suspension electrophoresis. | 1977 | 848738 | |
| control of fatty acid composition of acholeplasma laidlawii membranes. | the temperature-dependent pattern of incorporation of palmitate and oleate from the growth medium into acholeplasma laidlawii membrane lipids correlates with the physical state of the membrane defined by calorimetry. both the pattern and the state can be changed at will by changing the fatty acid composition of the membrane lipids. the ratio of palmitate to oleate incorporated is independent of temperature when the membrane bilayer is below its transition and fully ordered, but becomes temperatu ... | 1977 | 851532 |
| [some biochemical properties of acholeplasma laidlawii mutants defective in glucose carrier]. | some membrane properties of glucose transport system of acholeplasma laidlawii mutants uncapable of uptaking glucose as well as those of its non-metabolizing analogue, 3-0-methylglucose are studied. in contrast to the wild strain the mutant ptl-s cells fail to uptake carbohydrates with the saturation. there is no competition between carbohydrates during transport in this mutant no sugar exit is observed, and the sensitivity of transport to thiol reagents and pronase b is considerably affected. a ... | 1977 | 851551 |