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antibacterial activity of hen egg white lysozyme modified by heat and enzymatic treatments against oenological lactic acid bacteria and acetic acid bacteria.the antimicrobial activity of heat-denatured and hydrolyzed hen egg white lysozyme against oenological lactic acid and acetic acid bacteria was investigated. the lysozyme was denatured by heating, and native and heat-denatured lysozymes were hydrolyzed by pepsin. the lytic activity against micrococcus lysodeikticus of heat-denatured lysozyme decreased with the temperature of the heat treatment, whereas the hydrolyzed lysozyme had no enzymatic activity. heat-denatured and hydrolyzed lysozyme prep ...201425285490
control of spoilage fungi by protective lactic acid bacteria displaying probiotic properties.thirty-six lactic acid bacteria belong to lactococcus, lactobacillus, enterococcus, and pediococcus were isolated, and the spectrum of antifungal activity was verified against fusarium oxysporum (kacc 42109), aspergillus niger (kacc 42589), fusarium moniliforme (kacc 08141), penicillium chrysogenum (nii 08137), and the yeast candida albicans (mtcc 3017). three isolates, identified as pediococcus pentosaceus (tg2), lactobacillus casei (dy2), and lactococcus (bsn) were selected further, and their ...201424532445
antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production.in this study, 13 lactic acid bacteria (lab) strains (including 5 lactobacillus casei, 2 lactobacillus rhamnosus, 2 lactobacillus fermentum, 1 lactobacillus acidophilus, 1 lactobacillus plantarum, 1 lactobacillus sakei, and 1 lactobacillus reuteri species) were assessed for both their antifungal activity against four food spoilage molds (colletotrichum gloeosporioides, botrytis cinerea, penicillium expansum, and aspergillus flavus) and their capability to produce the novel antimicrobial compound ...201424412414
coexpression and secretion of endoglucanase and phytase genes in lactobacillus reuteri.a multifunctional transgenic lactobacillus with probiotic characteristics and an ability to degrade β-glucan and phytic acid (phytate) was engineered to improve nutrient utilization, increase production performance and decrease digestive diseases in broiler chickens. the bacillus subtilis wl001 endoglucanase gene (celw) and aspergillus fumigatus wl002 phytase gene (phyw) mature peptide (phywm) were cloned into an expression vector with the lactate dehydrogenase promoter of lactobacillus casei an ...201425050780
functional genomics of lactobacillus casei establishment in the gut.although the composition of the gut microbiota and its symbiotic contribution to key host physiological functions are well established, little is known as yet about the bacterial factors that account for this symbiosis. we selected lactobacillus casei as a model microorganism to proceed to genomewide identification of the functions required for a symbiont to establish colonization in the gut. as a result of our recent development of a transposon-mutagenesis tool that overcomes the barrier that h ...201425024222
simultaneous production of biosurfactants and bacteriocins by probiotic lactobacillus casei mrtl3.lactic acid bacteria (lab) are ubiquitous and well-known commensal bacteria in the human and animal microflora. lab are extensively studied and used in a variety of industrial and food fermentations. they are widely used for humans and animals as adjuvants, probiotic formulation, and dietary supplements and in other food fermentation applications. in the present investigation, lab were isolated from raw milk samples collected from local dairy farms of haryana, india. further, the isolates were s ...201424669225
generation of food-grade recombinant lactobacillus casei delivering myxococcus xanthus prolyl endopeptidase.prolyl endopeptidases (pep) (ec 3.4.21.26), a family of serine proteases with the ability to hydrolyze the peptide bond on the carboxyl side of an internal proline residue, are able to degrade immunotoxic peptides responsible for celiac disease (cd), such as a 33-residue gluten peptide (33-mer). oral administration of pep has been suggested as a potential therapeutic approach for cd, although delivery of the enzyme to the small intestine requires intrinsic gastric stability or advanced formulati ...201424752841
utilization of d-ribitol by lactobacillus casei bl23 requires a mannose-type phosphotransferase system and three catabolic enzymes.lactobacillus casei strains 64h and bl23, but not atcc 334, are able to ferment d-ribitol (also called d-adonitol). however, a bl23-derived ptsi mutant lacking enzyme i of the phosphoenolpyruvate:carbohydrate phosphotransferase system (pts) was not able to utilize this pentitol, suggesting that strain bl23 transports and phosphorylates d-ribitol via a pts. we identified an 11-kb region in the genome sequence of l. casei strain bl23 (lcabl_29160 to lcabl_29270) which is absent from strain atcc 33 ...201323564164
validation of drop plate technique for bacterial enumeration by parametric and nonparametric tests.drop plate technique has a priority and preference compared with the spread plate procedure, because of less time, quantity of media, effort requirement, little incubator space, and less labor intensive. the objective of this research was to compare the accuracy and fidelity of drop plate method vs. spread plate method by parametric and nonparametric statistical tests. for bacterial enumeration by drop and spread plate methods, successive dilutions of second subculture of lactobacillus casei and ...201325653794
effect of the combined probiotics with aflatoxin b₁-degrading enzyme on aflatoxin detoxification, broiler production performance and hepatic enzyme gene expression.in order to degrade aflatoxin b₁ (afb₁), afb₁-degrading microbes (probiotics) such as lactobacillus casei, bacillus subtilis and pichia anomala, and the afb₁-degrading enzyme from aspergillus oryzae were selected and combined to make feed additive. seventy-five 43-day-old male arbor acres broilers were randomly divided into 5 groups, 15 broilers for each group. the broilers were given with 5 kinds of diets such as the basal diet, 400 μg/kg afb₁ supplement without feed additive, and 200, 400, 800 ...201323831311
antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms.the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ...201324077540
screening and scoring of antimicrobial and biological activities of italian vulnerary plants against major oral pathogenic bacteria.this study aims to evaluate the activity of italian vulnerary plants against the most important oral pathogenic bacteria. this estimate was accomplished through a fivefold process: (a) a review of ethnobotanical and microbiological data concerning the italian vulnerary plants; (b) the development of a scoring system to rank the plants; (c) the comparative assessment of microbiological properties; (d) the assessment of potential cytotoxic effects on keratinocyte-like cells and gingival fibroblast ...201324302963
incorporation of antimicrobial agents can be used to enhance the antibacterial effect of endodontic sealers.the antibacterial activity of five endodontic sealers against three different microorganism strains alone and following incorporation of 2% benzalkonium chloride (bc) and 2% cetylpyridinium chloride (cpc) was evaluated.201323107192
the diversity of intestinal microbiota of mongolians living in inner mongolia, china.the mongolian nationality has developed their unique lifestyle and dietary habit for thousands of years. however, by now, little research has been focused on mongolian gut microbiota and how it is related to different dietary habits. in this study, denaturing gradient gel electrophoresis (dgge) and quantitative polymerase chain reaction (qpcr) methods were applied to reveal the diversity of predominant gut bacteria of 48 healthy mongolians recruited from hohhot city and the xilin gol pasturing a ...201324311315
the effect of lacto- and bifidobacteria in monoculture on the vaginal microflora in norm and in cases of intravaginal staphylococcosis.the influence of probiotic strains of lactobacillus casei imv b-7280, lactobacillus acidophilus imv b-7279, bifidobacterium animalis vkl, lactobacillus delbrueckii subsp. bulgaricus imv b-7281 and bifidobacterium animalis vkb, each strain separately, to the range of the urogenital tract microflora in physiological norm and in cases of experimental intravaginal staphylococcosis of mice induced by staphylococcus aureus 8325-4. it was found that all these strains had different efficiency in staphyl ...201323866586
assessment of the effect of stress-tolerance acquisition on some basic characteristics of specific probiotics.the production of viable functional probiotics presupposes stability of strain features in the final product. we evaluated the impact of acquisition of heat-tolerance and subsequent freeze-drying on the adhesion properties of lactobacillus rhamnosus gg, lactobacillus casei shirota, bifidobacterium lactis bb-12 and bifidobacterium animalis if20/1 and on their ability to inhibit the adhesion of pathogens in a mucus model. both fresh and freeze-dried cultures were evaluated. significant differences ...201323688551
bioactivity of probiotic whey cheese: characterization of the content of peptides and organic acids.probiotic whey cheeses have been produced for several years. it is recognized that several bacterium-mediated metabolic activities contribute differently to the final sensory and nutritional profiles of dairy products. hence the metabolic activity of probiotic strains in a whey cheese and their contribution to the bioactivity of such matrices were investigated here, including in particular bifidobacterium animalis, lactobacillus acidophilus and lactobacillus casei.201323400948
role of probiotics in the prevention and treatment of meticillin-resistant staphylococcus aureus infections.meticillin-resistant staphylococcus aureus (mrsa) is a multidrug-resistant micro-organism and is the principal nosocomial pathogen worldwide. following initial in vitro experiments demonstrating that lactobacillus acidophilus cl1285(®) and lactobacillus casei lbc80r(®) commercial strains exhibit antibacterial activity against clinical mrsa isolates, we conducted a literature search to find any evidence of probiotic efficacy in decolonisation or treatment of s. aureus infection. as summarised bel ...201324071026
the applications of polysaccharides from various mushroom wastes as prebiotics in different systems.the bases or stipes of mushrooms are normally discarded as low-economic value animal feed and compost. there are no known reports on deriving polysaccharides from these mushroom wastes for use as prebiotics. this study showed that the relatively low concentration (0.1% to 0.5%) of polysaccharides from lentinula edodes stipe, pleurotus eryngii base, and flammulina velutipes base can enhance the survival rate of lactobacillus acidophilus, lactobacillus casei, and bifidobacterium longum subsp. long ...201323701736
antipathogenic activity of probiotics against salmonella typhimurium and clostridium difficile in anaerobic batch culture systems: is it due to synergies in probiotic mixtures or the specificity of single strains?probiotics are currently being investigated for prevention of infections caused by enteric pathogens. the aim of this in vitro study was to evaluate the influence of three single probiotics: lactobacillus casei ncimb 30185 (pxn 37), lactobacillus acidophilus ncimb 30184 (pxn 35), bifidobacterium breve ncimb 30180 (pxn 25) and a probiotic mixture containing the above strains plus twelve other strains belonging to the lactobacillus, bifidobacterium, lactococcus, streptococcus and bacillus genera o ...201324091275
epithelial cell proliferation arrest induced by lactate and acetate from lactobacillus casei and bifidobacterium breve.in an attempt to identify and characterize how symbiotic bacteria of the gut microbiota affect the molecular and cellular mechanisms of epithelial homeostasis, intestinal epithelial cells were co-cultured with either lactobacillus or bifidobacterium as bona fide symbionts to examine potential gene modulations. in addition to genes involved in the innate immune response, genes encoding check-point molecules controlling the cell cycle were among the most modulated in the course of these interactio ...201323646174
fermentation of non-sterilized fish biomass with a mixed culture of film-forming yeasts and lactobacilli and its effect on innate and adaptive immunity in mice.non-sterilized fish waste containing fish bones was fermented using combined starter cultures of film-forming yeast (candida ethanolica) and lactic acid bacteria (lab; lactobacillus casei and lactobacillus rhamnosus) in order to obtain a liquefied fermented broth without spoiling. during the entire fermentation, the number of lab cells was maintained at a high level (6 × 10(8)-5 × 10(7) cells/ml). although the number of general bacteria was 10(6)cell/ml after adding non-sterilized fish biomass, ...201323810659
role of lactobacillus in the prevention of clostridium difficile-associated diarrhea: a meta-analysis of randomized controlled trials.clostridium difficile-associated diarrhea (cdad) is a major public health problem because of significant morbidity and mortality, and many clinicians pay attention to lactobacillus as a potentially effective treatment. the purpose of this meta-analysis was to evaluate the efficacy of lactobacillus in the prevention of cdad.201324229690
bacteriocin pj4 active against enteric pathogen produced by lactobacillus helveticus pj4 isolated from gut microflora of wistar rat (rattus norvegicus): partial purification and characterization of bacteriocin.the increase of multidrug-resistant pathogens and the restriction on the use antibiotics due to its side effects have drawn attention to the search for possible alternatives. bacteriocins are small antimicrobial peptides produced by numerous bacteria. much interest has been focused on bacteriocins because they exhibit inhibitory activity against pathogens. lactic acid bacteria possess the ability to synthesize antimicrobial compounds (like bacteriocin) during their growth. in this study, an anti ...201323371780
effect of probiotic species on irritable bowel syndrome symptoms: a bring up to date meta-analysis.immune system alteration in irritable bowel syndrome (ibs) patients may be modulated by probiotics. we assessed the efficacy of some probiotic species in alleviating characteristic ibs symptoms.201323548007
antimicrobial effects of silver zeolite, silver zirconium phosphate silicate and silver zirconium phosphate against oral microorganisms.to evaluate the antimicrobial activities of silver inorganic materials, including silver zeolite (agz), silver zirconium phosphate silicate (agzrpsi) and silver zirconium phosphate (agzrp), against oral microorganisms. in line with this objective, the morphology and structure of each type of silver based powders were also investigated.201323570016
construction and potential application of controlled autolytic systems for lactobacillus casei in cheese manufacture.the rapid release of intracellular enzymes into the curd by the autolysis of lactic acid bacteria starters is universally recognized as a critical biological process to accelerate cheese ripening. lactobacillus casei is typically the dominant nonstarter lactic acid bacterium in the ripening cheese. in this study, two controlled autolytic systems were established in l. casei bl23, based on the exploitation of the autolysins sourced from lactococcus lactis (acma) and enterococcus faecalis (atla). ...201323834793
detection, partial purification and characterization of bacteriocin produced by lactobacillus brevis fptlb3 isolated from freshwater fish: bacteriocin from lb. brevis fptlb3.lactobacillus brevis fptlb3 was isolated from freshwater fish, capable of producing bacteriocin that had broad spectrum of inhibition (3200 au/ml) against escherichia coli mtcc 1563, enterococcus faecalis mtcc 2729, lactobacillus casei mtcc 1423, lactobacillus sakei atcc 15521 and staphylococcus aureus atcc 25923. the antimicrobial activity of crude supernatant fluid was stable after heating at 121 °c for 60 min and declined thereafter. stability of antimicrobial activity was observed at ph rang ...201324425883
a novel type of peptidoglycan-binding domain highly specific for amidated d-asp cross-bridge, identified in lactobacillus casei bacteriophage endolysins.peptidoglycan hydrolases (pghs) are responsible for bacterial cell lysis. most pghs have a modular structure comprising a catalytic domain and a cell wall-binding domain (cwbd). pghs of bacteriophage origin, called endolysins, are involved in bacterial lysis at the end of the infection cycle. we have characterized two endolysins, lc-lys and lc-lys-2, identified in prophages present in the genome of lactobacillus casei bl23. these two enzymes have different catalytic domains but similar putative ...201323733182
a review on traditional turkish fermented non-alcoholic beverages: microbiota, fermentation process and quality characteristics.shalgam juice, hardaliye, boza, ayran (yoghurt drink) and kefir are the most known traditional turkish fermented non-alcoholic beverages. the first three are obtained from vegetables, fruits and cereals, and the last two ones are made of milk. shalgam juice, hardaliye and ayran are produced by lactic acid fermentation. their microbiota is mainly composed of lactic acid bacteria (lab). lactobacillus plantarum, lactobacillus brevis and lactobacillus paracasei subsp. paracasei in shalgam fermentati ...201323859403
putrescine production from different amino acid precursors by lactic acid bacteria from wine and cider.the aim of this work was to study the production of biogenic amines and particularly putrescine in lactic acid bacteria (lab) related to wine and cider. we applied an analytical protocol that involves the use of pcr and tlc techniques to determine the production of putrescine from different precursors. moreover, we also studied the ability of the lactobacillus and pediococcus tested to produce histamine and tyramine. the results showed that the majority of the lactobacillus brevis analyzed harbo ...201323685467
protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying e7 antigen at its surface.human papillomavirus (hpv) is the causative agent of cervical cancer (cxca) and the most commonly sexually transmitted pathogen worldwide. hpv type 16 (hpv-16) e7 oncoprotein is constitutively produced in cxca and considered as a good antigen candidate for the development of new therapeutic cxca vaccines. here, we report the use of non-genetically modified, e7-expressing lactic acid bacteria (lab) by using the cell-binding domain from lactobacillus casei a2 phage lysin as a cell wall anchor. the ...201323212671
cloning and expression of a codon-optimized gene encoding the influenza a virus nucleocapsid protein in lactobacillus casei.lactic acid bacteria (lab) species are envisioned as promising vehicles for the mucosal delivery of therapeutic and prophylactic molecules, including the development of oral vaccines. in this study, we report on the expression of a synthetic nucleocapsid (np) gene of influenza a virus in lactobacillus casei. the np gene was re-designed based on the trna pool and the codon usage preference of l. casei bl23. the codon-optimized np gene was then cloned and expressed in l. casei rceid02 under the co ...201324400527
double-blind, randomised clinical assay to evaluate the efficacy of probiotics in preterm newborns weighing less than 1500 g in the prevention of necrotising enterocolitis.a randomised, double-blind clinical trial was undertaken in order to assess the effectiveness of probiotics in the prevention of necrotising enterocolitis (nec) in newborns weighing <1500 g.201322556209
characterization of putative class ii bacteriocins identified from a non-bacteriocin-producing strain lactobacillus casei atcc 334.several putative class ii bacteriocin-like genes were identified in lactobacillus casei atcc 334, all of which might encode peptides with a double-glycine leader. six peptides encoded by these genes were heterologously expressed in escherichia coli and then partially purified in order to test their bacteriocin activity. the results revealed that the mature lsei_2163 peptide was a class iid bacteriocin that exhibited antimicrobial activity against some lactobacilli and several listeria species. s ...201322688903
[influence of lactobacillus casei on biofilm formation by enteroaggregative escherichia coli strains isolated from irritable bowel syndrome (ibs)].many pathogenic bacterial species have the ability to biofilm formation. in our study we determined the influence of lactobacillus casei on biofilm formation by enteroaggregative escherichia coli (eaec) strains obtained from irritable bowel syndrome patients.201324180127
osmotic stress adaptation in lactobacillus casei bl23 leads to structural changes in the cell wall polymer lipoteichoic acid.the probiotic gram-positive bacterium lactobacillus casei bl23 is naturally confronted with salt-stress habitats. it has been previously reported that growth in high-salt medium, containing 0.8 m nacl, leads to modifications in the cell envelope of this bacterium. in this study, we report that l. casei bl23 has an increased ability to form biofilms and to bind cations in high-salt conditions. this behaviour correlated with modifications of surface properties involving teichoic acids, which are i ...201324014660
distribution dynamics of recombinant lactobacillus in the gastrointestinal tract of neonatal rats.one approach to deliver therapeutic agents, especially proteins, to the gastro-intestinal (gi) tract is to use commensal bacteria as a carrier. genus lactobacillus is an attractive candidate for use in this approach. however, a system for expressing exogenous proteins at a high level has been lacking in lactobacillus. moreover, it will be necessary to introduce the recombinant lactobacillus into the gi tract, ideally by oral administration. whether orally administered lactobacillus can reach and ...201323544119
advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic escherichia coli-mediated inflammation.previously, a bovine intestinal epithelial cell line (bie cells) was successfully established. this work hypothesized that bie cells are useful in vitro model system for the study of interactions of microbial- or pathogen-associated molecular patterns (mamps or pamps) with bovine intestinal epithelial cells and for the selection of immunoregulatory lactic acid bacteria (lab).201323497067
compound(s) secreted by lactobacillus casei strain shirota yit9029 irreversibly and reversibly impair the swimming motility of helicobacter pylori and salmonella enterica serovar typhimurium, respectively.we conducted experiments in order to examine whether the probiotic lactobacillus casei strain shirota yit9029 (lcs) in vitro and in vivo antagonism of helicobacter pylori and salmonella, involves inhibition of the swimming motility of these pathogens. we report the irreversible inhibition of the swimming motility of h. pylori strain 1101 and reversible inhibition of salmonella enterica serovar typhimurium (s. typhimurium) strain sl1344 by compound(s) secreted by lcs. in h. pylori 1101, irreversi ...201323873784
comparative therapeutic effect of probiotic lactobacillus casei alone and in conjunction with antiprotozoal drugs in murine giardiasis.various antiprotozoal drugs have been used to counteract the spread of giardiasis. however, due to increase in resistance to these compounds, there is an urgent need to find a natural biocompatible product to fight the pathogen in more healthy and effective way. the present study was designed to compare the therapeutic effect of probiotic lactobacillus casei alone and in conjunction with antiprotozoal drugs on the outcome of giardiasis in murine model. balb/c mice were challenged with giardia in ...201323559378
oral administration of the probiotic lactobacillus casei ameliorates gut morphology and physiology in malnourished-giardia intestinalis-infected balb/c mice.malnutrition reduces the host immunity and enhances the host susceptibility to various diseases. the present study describes the effect of oral administration of probiotic lactobacillus casei to malnourished-giardia-infected balb/c mice with respect to surface alterations and brush border membrane enzyme activity of the small intestine. it was observed that probiotic feeding either prior to or simultaneously with giardia infection to malnourished mice led to significantly enhanced activity of di ...201327335861
[identification of lactic acid bacteria in commercial yogurt and their antibiotic resistance].to identify lactic acid bacteria (lab) in commercial yogurts and investigate their antibiotic resistance.201324341282
lactobacillus acidophilus la5, bifidobacterium bb12, and lactobacillus casei dn001 modulate gene expression of subset specific transcription factors and cytokines in peripheral blood mononuclear cells of obese and overweight people.probiotics are believed to have interaction with immune cells through sustained effects on gene expression of different cytokines and transcription factors. the present randomized doubled-blind controlled clinical trial was performed recruiting 75 individuals with bmi 25-35, who were randomly assigned to the following three groups: group 1 (n = 25) who consumed regular yogurt as part of a low calorie diet [rlcd], group 2 (n = 25) who received probiotic yogurt with a lcd [plcd] and group 3 (n = 2 ...201324019207
anticariogenic and phytochemical evaluation of eucalyptus globules labill.in the present study, in vitro anticariogenic potential of ethyl acetate, hexane and methanol and aqueous extracts of plant leaves of eucalyptus globules labill. were evaluated by using four cariogenic bacteria, lactobacillus acidophilus, lactobacillus casei, staphylococcus aureus and streptococcus mutans. agar well diffusion method and minimum inhibitory concentration (mic) were used for this purpose. the ethyl acetate extracted fraction of plant leaves showed good inhibitory effects against al ...201323961222
effects of 2 bracket and ligation types on plaque retention: a quantitative microbiologic analysis with real-time polymerase chain reaction.the aim of this study was to evaluate the effects of self-ligating brackets and conventional brackets ligated with stainless steel ligatures on dental plaque retention and microbial flora.201323910207
daily consumption of a synbiotic yogurt decreases energy intake but does not improve gastrointestinal transit time: a double-blind, randomized, crossover study in healthy adults.probiotic and synbiotic products are widely marketed to healthy individuals, although potential benefits for these individuals are rarely studied. this study investigated the effect of daily consumption of a synbiotic yogurt on gastrointestinal (gi) function in a sample of healthy adults.201323787118
efficacy of polymer coating of probiotic beads suspended in pressurized and pasteurized longan juices on the exposure to simulated gastrointestinal environment.alginate-coated lactobacillus acidophilus la5 or lactobacillus casei 01 was recoated with either 0.1-0.5% (w/v) alginate or 0.05-0.15% (w/v) poly-l-lysine (pll) plus 0.2% (w/v) alginate or 5-15% (w/v) gelatin, after which they were determined for survivability in gastric or bile longan juices. the morphology of encapsulated probiotic cells illustrated that recoated beads with 0.5% alginate showed a more compact surface and a greater protective effect than other recoating materials. the recoated ...201323701113
use of lactobacillus acidophilus and lactobacillus casei for a potential probiotic legume-based fermented product using pigeon pea (cajanus cajan).the aim of the present study was to evaluate the use of pigeon pea (cajanus cajan) as an appropriate substrate in the production of a legume-based fermented product with lactobacillus acidophilus atcc 314 or lactobacillus casei atcc 393 and then to ascertain the effects of the addition of ingredients such as powdered milk and banana or strawberry sauce. the products were analyzed for viable cell counts, ph, and sensory attributes during product manufacture and throughout the refrigerated storage ...201323433374
activities of free and encapsulated lactobacillus acidophilus la5 or lactobacillus casei 01 in processed longan juices on exposure to simulated gastrointestinal tract.fruit drinks containing probiotics are gaining interest in the global marketplace. for example, longan juice, containing carbohydrate and various bioactive components, is a potentially health-promoting beverage as well as probiotic carrier for human consumption. in this study, high-pressure and thermal processes were applied to eliminate competitive micro-organisms in longan juice prior to the addition of lactobacillus acidophilus la5 or lactobacillus casei 01. the activities of these probiotics ...201323401169
differences in tlr9-dependent inhibitory effects of h(2)o(2)-induced il-8 secretion and nf-kappa b/i kappa b-alpha system activation by genomic dna from five lactobacillus species.lactic acid bacteria (lab) show anti-inflammatory effects, and their genomic dna was identified as one of the anti-inflammatory components. despite the differences in anti-inflammatory effects between live lab dependent not only on genus but also species, this effect has not been compared at the genomic dna level. we compared the anti-inflammatory effects of the genomic dna from five lactobacillus species-lactobacillus acidophilus, lactobacillus casei, lactobacillus gasseri, lactobacillus planta ...201323182970
effect of supplementation of probiotics and phytosterols alone or in combination on serum and hepatic lipid profiles and thyroid hormones of hypercholesterolemic rats.probiotic bacteria and phytosterols are natural hypocholesterolemic agents with potential cardiovascular benefits. accordingly, the present study was conducted to evaluate the effect of supplementation of probiotics and phytosterols alone or in combination on serum and hepatic lipid profiles and thyroid hormones of hypercholesterolemic rats. mixed probiotics treatment consisted of 8 probiotic strains: 2 strains of each of lactobacillus acidophilus, lactobacillus casei, lactobacillus gasseri, and ...201323182355
the effect of nacl substitution with kcl on proteinase activities of cell-free extract and cell-free supernatant at different ph levels and salt concentrations: lactobacillus acidophilus and lactobacillus casei.the aim of this study was to investigate the effect of substitution of nacl with kcl at different ph levels and salt concentrations on proteinase activity of cell-free extract and cell-free supernatant of the probiotics lactobacillus acidophilus and lactobacillus casei. de man, rogosa, and sharpe broth aliquots were mixed with 2 pure salts (nacl and kcl) and 2 salt concentrations at 2 concentration levels (5 and 10%), inoculated with lactobacillus acidophilus or lactobacillus casei, and incubate ...201323084889
lactobacillus casei and lactobacillus acidophilus regulate inflammatory pathway and improve antioxidant status in collagen-induced arthritic rats.in view of well-established immunomodulatory properties of lactobacillus, present investigation was carried out to evaluate antioxidant and anti-inflammatory potential of lactobacillus casei and lactobacillus acidophilus, against inflammatory pathway and oxidative stress developed in an experimental model of arthritis. collagen-induced arthritis (cia) model was used. oral administration of l. casei, l. acidophilus, standard antiarthritic drug indomethacin, and vehicle were started after induced ...201323030670
comparative genome analysis of lactobacillus casei strains isolated from actimel and yakult products reveals marked similarities and points to a common origin.the members of the lactobacillus genus are widely used in the food and feed industry and show a remarkable ecological adaptability. several lactobacillus strains have been marketed as probiotics as they possess health-promoting properties for the host. in the present study, we used two complementary next-generation sequencing technologies to deduce the genome sequences of two lactobacillus casei strains lca and lcy, which were isolated from the products actimel and yakult, commercialized as prob ...201323815335
heterologous expression of lactobacillus casei reco improved the multiple-stress tolerance and lactic acid production in lactococcus lactis nz9000 during salt stress.the aim of this study was to investigate the effect of nisin-inducible reco expression on the stress tolerance of lactococcus lactis nz9000. reco protein from lactobacillus casei zhang was introduced into lactococcus lactis nz9000 by using a nisin-inducible expression system. the recombinant strain (nz-reco) exhibited higher growth performances and survival rate compared with the control strain (nz-vector) under stress conditions. in addition, the nz-reco strain exhibited 1.37-, 1.41-, and 1.42- ...201323796607
synbiotic impact of tagatose on viability of lactobacillus rhamnosus strain gg mediated by the phosphotransferase system (pts).synbiotics, the combination of prebiotics and probiotics, has been shown to produce synergistic effects that promote gastrointestinal well-being of host. tagatose is a low calorie food ingredient with putative health-promoting benefits. herein, we investigated its synbiotic impact on the viability of lactobacillus casei 01 and lactobacillus rhamnosus strain gg and the potential mechanism involved. tagatose, as a synbiotic substrate, enhanced the growth of l. casei 01 and l. rhamnosus strain gg c ...201323764214
probiotics promote rapid-turnover protein production by restoring gut flora in patients with alcoholic liver cirrhosis.accumulating evidence suggests that deterioration of the gut flora contributes to the pathogenesis of alcoholic liver cirrhosis (alc). however, the alc flora profile and its response to probiotic treatment have not been fully examined. this double-blind placebo-controlled study aimed to evaluate whether the probiotic beverage yakult 400 (y400), which contains lactobacillus casei strain shirota, improves liver function in alc patients, and to analyze the precise gut flora profile by real-time qua ...201326201812
ca2+-citrate uptake and metabolism in lactobacillus casei atcc 334.the putative citrate metabolic pathway in lactobacillus casei atcc 334 consists of the transporter cith, a proton symporter of the citrate-divalent metal ion family of transporters citmhs, citrate lyase, and the membrane-bound oxaloacetate decarboxylase complex oad-abdh. resting cells of lactobacillus casei atcc 334 metabolized citrate in complex with ca(2+) and not as free citrate or the mg(2+)-citrate complex, thereby identifying ca(2+)-citrate as the substrate of the transporter cith. the pat ...201323709502
probiotics lactobacillus reuteri dsm 17938 and lactobacillus casei crl 431 modestly increase growth, but not iron and zinc status, among indonesian children aged 1-6 years.probiotics and milk calcium may increase resistance to intestinal infection, but their effect on growth and iron and zinc status of indonesian children is uncertain. we investigated the hypotheses that cow milk with added probiotics would improve growth and iron and zinc status of indonesian children, whereas milk calcium alone would improve growth but reduce iron and zinc status. a 6-mo randomized trial was conducted in low-socioeconomic urban communities of jakarta. healthy children (n = 494) ...201323700339
novel accurate bacterial discrimination by maldi-time-of-flight ms based on ribosomal proteins coding in s10-spc-alpha operon at strain level s10-germs.matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is one of the most widely used mass-based approaches for bacterial identification and classification because of the simple sample preparation and extremely rapid analysis within a few minutes. to establish the accurate maldi-tof ms bacterial discrimination method at strain level, the ribosomal subunit proteins coded in the s10-spc-alpha operon, which encodes half of the ribosomal subunit protein and is hi ...201323686278
nonstarter lactic acid bacteria volatilomes produced using cheese components.in long-ripened cheese, flavor formation occurs during ripening. the metabolism of lactic acid bacteria (lab) leads to the production of different compounds that contribute to the flavor of cheese. the contribution of lab to the formation of cheese flavor has previously been studied. however, the specific nonstarter lab (nslab) metabolic reactions in ripened cheese that lead to the formation of flavor compounds remain unclear. in ripened cheese, the nutrient sources available include small pepti ...201323684038
the antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of shigella sonnei and shigella flexneri in vitro.shigellosis remains an important public health problem in developing countries with s. sonnei and s. flexneri in us, europe and in asian countries being of importance.201323682323
lactobacillus casei hy7213 ameliorates cyclophosphamide-induced immunosuppression in mice by activating nk, cytotoxic t cells and macrophages.lactic acid bacteria (lab) have recently attracted considerable attention as treatment options for immune diseases, the incidence of which has been increasing worldwide. the ability of tumor necrosis factor-α producing lab isolated from cheese to inhibit nf-κb activation in lipopolysaccharide (lps)-stimulated peritoneal macrophages was investigated. among the tested lab, lactobacillus casei hy7213 inhibited nf-κb activation most potently. therefore, we measured its immunopotentiating effect in c ...201323672525
immunomodulatory and anti-allergic effects of orally administered lactobacillus species in ovalbumin-sensitized mice.we investigated the effects of orally administered probiotic bacteria (lactobacillus species) as allergic immune modulators in ovalbumin (ova)-sensitized mice. balb/c mice were intraperitoneally injected with ova twice at a 2-week interval for allergy sensitization. the mice were then orally administered lactobacillus casei yit9029 (l1), l. casei hy7201 (l2), l. brevis hy7401 (l3), or l. plantarum hy20301 (l4) every 2 days for 3 weeks. total ige levels significantly decreased in sera of l3-admin ...201323648865
significant effect of ca2+ on improving the heat resistance of lactic acid bacteria.the heat resistance of lactic acid bacteria (lab) has been extensively investigated due to its highly practical significance. reconstituted skim milk (rsm) has been found to be one of the most effective protectant wall materials for microencapsulating microorganisms during convective drying, such as spray drying. in addition to proteins and carbohydrate, rsm is rich in calcium. it is not clear which component is critical in the rsm protection mechanism. this study investigated the independent ef ...201323617813
sonicated pineapple juice as substrate for l. casei cultivation for probiotic beverage development: process optimisation and product stability.the aim of this study was to evaluate the use of sonicated pineapple juice as substrate for producing a probiotic beverage by lactobacillus casei nrrl b442. maximal microbial viability was found by cultivating l. casei at 31°c and ph 5.8 (optimised conditions). after fermentation, samples of sweetened and non-sweetened juice were stored. after 42 days of storage under refrigeration (4°c), the microbial viability was 6.03 log cfu/ml in the non-sweetened sample and 4.77 log cfu/ml in the sweetened ...201323561104
changes in selected parameters related to proteolysis during ageing of dry-cured pork loins inoculated with probiotics.the effect of inoculation with a probiotic strain, lactobacillus casei łock 0900, on selected parameters related to proteolysis of dry-cured pork loins during ageing was studied. moisture content decreased significantly (p<0.05) throughout ageing, accompanied by a progressive reduction in water activity. the total nitrogen (tn) content increased during ageing with no effect (p<0.05) of inoculation with l. casei on its level. the greatest increase in non-protein nitrogen (npn) content during agei ...201323561079
synthesis of fucosyl-n-acetylglucosamine disaccharides by transfucosylation using α-l-fucosidases from lactobacillus casei.alfb and alfc α-l-fucosidases from lactobacillus casei were used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. alfb and alfc activities exclusively produced fucosyl-α-1,3-n-acetylglucosamine and fucosyl-α-1,6-n-acetylglucosamine, respectively. the reaction kinetics showed that alfb can convert 23% p-nitrophenyl-α-l-fucopyranoside into fucosyl-α-1,3-n-acetylglucosamine and alfc at up to 56% into fucosyl-α-1,6-n-acetylglucosamine.201323542622
performance in nondairy drinks of probiotic l. casei strains usually employed in dairy products.the increase in vegetarianism as dietary habit and the increased allergy episodes against dairy proteins fuel the demand for probiotics in nondairy products. lactose intolerance and the cholesterol content of dairy products can also be considered two additional reasons why some consumers are looking for probiotics in other foods. we aimed at determining cell viability in nondairy drinks and resistance to simulated gastric digestion of commercial probiotic lactobacilli commonly used in dairy prod ...201323527588
monitoring survival of lactobacillus casei atcc 393 in probiotic yogurts using an efficient molecular tool.the aim of the present study was to monitor the survival of the probiotic strain lactobacillus casei atcc 393 during refrigerated storage of natural regular yogurts compared with lactobacillus delbrueckii ssp. bulgaricus. both free and immobilized cells on supports of high industrial interest, such as fruits and oat pieces, were tested. microbiological and strain-specific multiplex pcr analysis showed that both free and immobilized lb. casei atcc 393 were detected in the novel products at levels ...201323498002
characterization of a regulatory network of peptide antibiotic detoxification modules in lactobacillus casei bl23.two-component systems (tcs) are major signal transduction pathways that allow bacteria to detect and respond to environmental and intracellular changes. a group of tcs has been shown to be involved in the response against antimicrobial peptides (amps). these tcs are characterized by the possession of intramembrane-sensing histidine kinases, and they are usually associated with abc transporters of the peptide-7 exporter family (pep7e). lactobacillus casei bl23 encodes two tcs belonging to this gr ...201323455349
a defined-multispecies microbial model for studying enamel caries development.the aims of this study were to describe and validate an in vitro multispecies microbial biofilm model for caries development by evaluating the effects of varying medium concentration of sucrose (0.5 and 1.0%) and fluoride (0.4, 0.8 and 1.0 ppm f) in study 1, and calcium (1.0 and 2.0 mm ca) in study 2. defined-multispecies biofilms, formed by lactobacillus casei, streptococcus mutans, s. salivarius and s. sanguinis, were grown on the surface of salivary-pellicle-coated enamel slabs, with known ba ...201323446436
in vitro investigation of the immunomodulatory potential of probiotic lactobacillus casei.the current study investigated the immunomodulatory potential of ethyl acetate soluble supernatant of lactobacillus casei (lc-eas) in vitro. the effect of lc-eas on nitric oxide release was analyzed in raw 264.7 cells, wherein, an inhibition in nitric oxide production through suppression of inducible nitric oxide synthase mrna expression was observed. evaluation of lc-eas on lps-induced peripheral blood mononuclear cells showed a down-regulation in tnf-α and il-6 genes and an upregulation of il- ...201326782605
effects of a probiotic fermented milk on functional constipation: a randomized, double-blind, placebo-controlled study.evidence suggests that probiotics reduce certain constipation-related symptoms. lactobacillus casei strain shirota has never been tested as treatment for functional constipation in otherwise-healthy subjects. to evaluate the efficacy of this probiotic among adults with functional constipation was aimed.201323432408
complete genome sequence of the probiotic strain lactobacillus casei (formerly lactobacillus paracasei) lock919.lactobacillus casei is usually regarded as a bacterium that lives naturally in the human intestinal tract, where it can contribute to host health and well-being. we describe here the complete genome sequence of l. casei lock919, a strain with probiotic properties isolated from child feces. the genome consists of a 3.11-mb chromosome and a 29,768-bp plasmid.201324072862
impact of lactose starvation on the physiology of lactobacillus casei gcrl163 in the presence or absence of tween 80.the global proteomic response of the nonstarter lactic acid bacteria lactobacillus casei strain gcrl163 under carbohydrate depletion was investigated to understand aspects of its survival following cessation of fermentation. the proteome of l. casei gcrl163 was analyzed quantitatively after growth in modified mrs (with and without tween 80) with different levels of lactose (0% lactose, starvation; 0.2% lactose, growth limiting; 1% lactose, non-growth-limited control) using gel-free proteomics. r ...201324066708
not only osmoprotectant: betaine increased lactate dehydrogenase activity and l-lactate production in lactobacilli.lactobacilli are commonly used for industrial production of polymer-grade l-lactic acid. the present study tested the tween 80 alternative betaine in l-lactate production by several industrial lactobacilli. in flask fermentation of lactobacillus casei, lactobacillus buchneri, lactobacillus lactis and lactobacillus rhamnosus, the betaine addition (2g/l) had similar osmoprotectant effect with tween 80 but had increased the lactate dehydrogenase activities and l-lactate production than tween 80 con ...201324035452
[microorganisms effect with probiotic potential in water quality and growth of the shrimp litopenaeus vannamei (decapoda: penaeidae) in intensive culture].the use of probiotics has gained acceptance in aquaculture, particularly in maintaining water quality and enhancing growth in organisms. this study analyzed the effect of the commercial (em, japan) natural product composed by (rhodopseudomonas palustris, lactobacillus plantarum, lactobacillus casei and saccharomyces cerevisiae) added to the water, in order to determine its effect in water quality, sediment and growth of l. vannamei under intensive culture. the evaluation included three treatment ...201324027919
diversity and dynamic of lactic acid bacteria strains during aging of a long ripened hard cheese produced from raw milk and undefined natural starter.the aim of this study was to explore diversity and dynamic of indigenous lab strains associated with a long ripened hard cheese produced from raw milk and undefined natural starter such as pdo grana padano cheese. samples of milk, curd, natural whey culture and cheeses (2nd, 6th, 9th and 13th months of ripening) were collected from 6 cheese factories in northern italy. dna was extracted from each sample and from 194 lab isolates. trna(ala)-23s rdna-rflp was applied to identify isolates. strain d ...201324010599
effect of chestnut extract and chestnut fiber on viability of potential probiotic lactobacillus strains under gastrointestinal tract conditions.the main challenge to probiotics, during their passage through the gastrointestinal tract, are the acidic gastric secretions of the stomach, and the bile salts released into the duodenum. the survival of the strains, in this phase, is strongly influenced by the food used for their delivery. this work is part of a project studying the development of novel food processes, based on the use of chestnuts from cultivar "castagna di montella". in detail, the effect of indigestible chestnut fiber and of ...201324010594
lactobacillus casei shirota enhances the preventive efficacy of soymilk in chemically induced breast cancer.soy foods are known to be effective for breast cancer prevention. the habitual consumption of soy isoflavones in combination with the probiotic lactobacillus casei shirota (lcs) was shown to decrease the risk of breast cancer occurrence in our previous population-based case-controlled study among japanese women. the present study aimed to elucidate the cooperative prevention mechanism of soymilk and lcs using an animal carcinogenic model. female sprague-dawley rats received a high-fat, ain-76a d ...201323992486
metabolomic approach assisted high resolution lc-esi-ms based identification of a xenobiotic derivative of fenhexamid produced by lactobacillus casei.fenhexamid is a widely used fungicide with one of the highest maximum tolerance limits approved for fruits and vegetables. the goal of this study was to examine if fenhexamid is metabolized by a nontarget organism, a lactobacillus species (lactobacillus casei shirota), a probiotic strain of the human gastrointestinal tract. the assignment of bacterial derivatives of the xenobiotic fenhexamid was substantially facilitated by a metabolomic software based approach optimized for the extraction of mo ...201323971653
dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain lactobacillus casei shirota.dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (uc) pathology. we determined the immunomodulatory effects of probiotic strain lactobacillus casei shirota (lcs) on human dc from healthy controls and active uc patients.201323970814
probiotic beverage with soy isoflavone consumption for breast cancer prevention: a case-control study.the purpose of this study is to evaluate how beverages containing lactobacillus casei shirota (bls) and soy isoflavone consumption since adolescence affected the incidence of breast cancer. in a population-based case-control study, three hundred and six cases with breast cancer and 662 controls aged 40 to 55 were matched for age and residential area and included in the analyses. diet, lifestyle and other breast cancer risk factors were investigated using the self-administered questionnaire and i ...201323966890
randomised clinical trial: mesalazine and/or probiotics in maintaining remission of symptomatic uncomplicated diverticular disease--a double-blind, randomised, placebo-controlled study.placebo-controlled studies in maintaining remission of symptomatic uncomplicated diverticular disease (sudd) of the colon are lacking.201323957734
genome sequence of the probiotic strain lactobacillus rhamnosus (formerly lactobacillus casei) lock900.lactobacillus rhamnosus lock900 fulfills the criteria required for probiotic strains. in this study, we report a whole-genome sequence of this isolate and compare it with other l. rhamnosus complete genome sequences already published.201323950137
antibacterial activity, surface roughness, flexural strength, and solubility of conventional luting cements containing chlorhexidine diacetate/cetrimide mixtures.the failure of fixed dental restorations is commonly associated with caries. the use of conventional luting cements containing antibacterial agents may overcome this problem.201323929372
genomic adaptation of the lactobacillus casei group.lactobacillus casei, l. paracasei, and l. rhamnosus form a closely related taxonomic group (lactobacillus casei group) within the facultatively heterofermentative lactobacilli. here, we report the complete genome sequences of l. paracasei jcm 8130 and l. casei atcc 393, and the draft genome sequence of l. paracasei com0101, all of which were isolated from daily products. furthermore, we re-annotated the genome of l. rhamnosus atcc 53103 (also known as l. rhamnosus gg), which we have previously r ...201324116025
lactobacillus casei strain shirota protects against nonalcoholic steatohepatitis development in a rodent model.gut microbiota alterations are associated with various disorders. in this study, gut microbiota changes were investigated in a methionine-choline-deficient (mcd) diet-induced nonalcoholic steatohepatitis (nash) rodent model, and the effects of administering lactobacillus casei strain shirota (lcs) on the development of nash were also investigated. mice were divided into three groups, given the normal chow diet (ncd), mcd diet, or the mcd diet plus daily oral administration of lcs for 6 wk. gut m ...201324113768
16s rrna pcr-denaturing gradient gel electrophoresis of oral lactobacillus casei group and their phenotypic appearances.this study aimed to develop a 16s rrna pcr-denaturing gradient gel electrophoresis (dgge) to identify the species level of lactobacillus casei group and to investigate their characteristics of acid production and inhibitory effect. pcr-dgge has been developed based on the 16s rrna gene, and a set of hda-1-gc and hda-2, designed at v2-v3 region, and another set of carp-1-gc and carp-2, designed at v1 region, have been used. the bacterial strains included l. casei atcc 393, l. paracasei ccug 32212 ...201324191230
the effect of a commercial probiotic drink containing lactobacillus casei strain shirota on oral health in healthy dentate people.in the past decade, the use of probiotic-containing products has been explored as a potential alternative in oral health therapy. a widely available probiotic drink, yakult, was evaluated for oral health applications in this longitudinal study. selected oral health parameters, such as levels and composition of salivary and tongue plaque microbiota and of malodorous gases, in dentate healthy individuals were investigated for changes. the persistence of the probiotic strain in the oral cavity was ...201324179468
viability of probiotic lactobacillus casei in yoghurt: defining the best processing step to its addition.probiotics are live microorganisms capable of producing beneficial effects on its host when consumed in adequate amounts. to exert these effects, foods must contain probiotic microorganisms in populations above 10(6) cfu/g or ml throughout its shelf life. one of the strategies to ensure high population of probiotics in fermented milk is to add them during or after the fermentation process separately from the starter cultures. the objective of this study was to investigate the behavior of the pro ...201324167959
molecular identification of lactobacillus spp. associated with puba, a brazilian fermented cassava food.puba or carimã is a brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. a total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de man, rogosa and sharpe agar (mrs); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomically by classical and molecular methods. in all samples, lactic acid bacteria were recovered as the dominant microbiota (7.86 ± 0.41 log10 cfu/g ...201324159278
growth inhibition of hepatocellular carcinoma huh7 cells by lactobacillus casei extract.lactobacillus casei (l. casei) is known to exert anti-proliferation effects on many types of cancer cells. however, the effect of l. casei on liver cancer has not been reported. accordingly, the aim of this study was to determine the anti-cancer effect of l. casei extract on huh7 cells.201323918568
decreased duration of acute upper respiratory tract infections with daily intake of fermented milk: a multicenter, double-blinded, randomized comparative study in users of day care facilities for the elderly population.there is insufficient evidence of preventive effect of probiotics on upper respiratory tract infections (urtis) in an elderly population.201323890374
[determination of folate content in ready-to-eat food products].in the last years, the consumption of ready-to-eat foods has become an increasing part of the current spanish diet. accordingly, the nutritional composition of these food categories should be investigated in order to estimate its contribution to vitamin and nutrient intakes, in particular its folate content. the broad lack of folate data in food composition tables and databases justifies this approach.201323889644
proteomic comparison of the probiotic bacterium lactobacillus casei zhang cultivated in milk and soy milk.soy milk is regarded as a substitute for milk and has become popular in varied diets throughout the world. it has been shown that a newly characterized probiotic bacterium (lactobacillus casei zhang) actually grows faster in soy milk than in bovine milk. to elucidate the mechanism involved, we carried out a proteomic analysis to characterize bacterial proteins that varied upon growth in soy milk and bovine milk at 3 different growth phases, and compare their expression under these conditions. a ...201323871367
strategies to decolorize high concentrations of methyl orange using growing cells of lactobacillus casei tistr 1500.batch, fed-batch, and continuous fermentation was used in the processing of methyl orange decolorization using growing cells of lactobacillus casei tistr 1500. this report presents the optimal conditions for methyl orange decolorization by the strain tistr 1500 in modified mrs via a central composite design (ccd) experiment. in particular, the highest decolorization efficiencies were obtained with 13.41 g/l of meat extract, and with 10.89 g/l of yeast extract at ph 6.88 at 35 °c. under the optim ...201324096665
oral delivery of a probiotic induced changes at the nasal mucosa of seasonal allergic rhinitis subjects after local allergen challenge: a randomised clinical trial.to determine effects of probiotic consumption on clinical and immunological parameters of seasonal allergic rhinitis (sar) in an out-of-season single nasal allergen challenge.201324260122
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