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medicinal attributes of major phenylpropanoids present in cinnamon.excessive production of free radicals has been implicated in many diseases including cancer. they are highly reactive and bring about oxidation of biomolecules i.e., proteins, lipids and nucleic acids which are associated with many degenerative diseases. natural products acting as antioxidants have ability to neutralize free radicals and their actions and hence they mitigate their harmful effects. the present study was designed to investigate pharmacological properties viz., antioxidant, antibac ...201627245453
antimicrobial and anti-quorum sensing activities of selected medicinal plants of ethiopia: implication for development of potent antimicrobial agents.traditional medicinal plants have been used as an alternative medicine in many parts of the world, including ethiopia. there are many documented scientific reports on antimicrobial activities of the same. to our knowledge, however, there is no report on the anti-quorum sensing (quorum quenching, qq) potential of traditional ethiopian medicinal plants. as many of the opportunistic pathogenic bacteria depend on quorum sensing (qs) systems to coordinate their virulence expression, interference with ...201627400878
vector potential of blattella germanica (l.) (dictyoptera: blattidae) for medically important bacteria at food handling establishments in jimma town, southwest ethiopia.cockroaches have been regarded as possible vectors of human enteropathogens. their presence and crawl particularly in food handling establishments could be risky for human health. therefore, this study was done to determine the vector potential of cockroach for medically important bacterial pathogens in restaurants and cafeterias. a cross-sectional study was conducted on cockroaches from restaurants and cafeterias in jimma town from may to september 2014. standard taxonomic keys and microbiologi ...201627294115
structural basis for latency and function of immune inhibitor a metallopeptidase, a modulator of the bacillus anthracis secretome.immune inhibitor a(inha)-type metallopeptidases are potential virulence factors secreted by members of the bacillus cereus group. two paralogs from anthrax-causing bacillus anthracis (bainha1 and bainha2) were shown to degrade host tissue proteins with broad substrate specificity. analysis of their activation mechanism and the crystal structure of a zymogenic bainha2 variant revealed a ∼750-residue four-domain structure featuring a pro-peptide, a catalytic domain, a domain reminiscent of viral e ...201626745529
detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, plutella xylostella (linnaeus), from india and its possible role in indoxacarb degradation.diamondback moth (dbm), plutella xylostella (linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. the insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. the present study aimed to determine the diversity of gut microflora in dbm, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. we screened 11 geographic populations of dbm i ...201626991291
intravenous drug abuse by patients inside the hospital: a cause for sustained bacteremia.patients with history of intravenous drug abuse are noted to be at risk of several infections including hiv, endocarditis, and other opportunistic infections. we report the case of a patient with sustained bacillus cereus bacteremia despite use of multiple antibiotic regimens during his inpatient stay. our case highlights the importance of high suspicion for active drug use inside the hospital in such patients. this is important in order to minimize unnecessary diagnostic workup and provide adeq ...201627433362
microbial diversity and biochemical characteristics of borassus akeassii wine.palm wine produced traditionally and consumed by many people in the south-west of burkina faso is subject to alteration. in this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. we monitored biochemical characteristics of fermenting wines as well as followed the microflora kinetics using culture-dependent and culture-independent methods. the analysis of the acid content and the bacterial population rev ...201627450560
phytosynthesis of silver nanoparticles using artemisia marschalliana sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties.a rapid phytosynthesis of silver nanoparticles (agnps) using an extract from the aerial parts of artemisia marschalliana sprengel was investigated in this study. the synthesized agnps using a. marschalliana extract was analyzed by uv-visible spectroscopy, x-ray diffraction, and fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. characteristic absorption bands of a ...201627199558
survey of the antibiofilm and antimicrobial effects of zingiber officinale (in vitro study).candidiasis is one of the most prevalent and important opportunistic fungal infections of the oral cavity caused by candida yeast species like candida albicans, c. glabrata, and c. krusei. in addition, several bacteria can cause oral infections. the inhibition of microbial biofilm is the best way to prevent oral infections.201627127591
chromosome-directed pcr-based detection and quantification of bacillus cereus group members with focus on b. thuringiensis serovar israelensis active against nematoceran larvae.bacillus thuringiensis serovar israelensis is a wide-spread soil bacterium affiliated with the b. cereus group (bcg) and is widely used in biocontrol products applied against mosquito and black fly larvae. for monitoring and quantification of applied b. thuringiensis serovar israelensis and its effect on indigenous b. thuringiensis serovar israelensis and bcg assemblages, efficient and reliable tools are essential. the abundance and properties of b. thuringiensis serovar israelensis strains in t ...201525979887
a novel multiplex pcr discriminates bacillus anthracis and its genetically related strains from other bacillus cereus group species.anthrax is an important zoonotic disease worldwide that is caused by bacillus anthracis, a spore-forming pathogenic bacterium. a rapid and sensitive method to detect b. anthracis is important for anthrax risk management and control in animal cases to address public health issues. however, it has recently become difficult to identify b. anthracis by using previously reported molecular-based methods because of the emergence of b. cereus, which causes severe extra-intestinal infection, as well as t ...201525774512
brown algae (phaeophyceae) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products.eight species of brown algae (phaeophyceae) from the coast of madagascar have been investigated for their chemical constituents. fucosterol (3) was obtained as the most abundant compound. the brown alga sargassum ilicifolium was the source for the first isolation of the terpenoid c27-alcohol 1,1',2-trinorsqualenol (1) from marine sources. from s. incisifolium we isolated the highly unsaturated glycolipid 1-o-palmitoyl-2-o-stearidonoyl-3-o-β-d-galactopyranosylglycerol (4) and we report the first ...201526358714
structural characterization and antimicrobial activity of a biosurfactant obtained from bacillus pumilus dsvp18 grown on potato peels.biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. they are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. major limiting factor in biosurfactant production is their production cost.201526495106
red algae (rhodophyta) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products.several species of red algae (rhodophyta) from the coastal regions of madagascar have been investigated for their natural products. the most abundant compound was cholesterol (5) in combination with a series of oxidized congeners. the brominated indoles 1-3 along with the sesquiterpene debilone (4) have been isolated from laurencia complanata. for the first time, debilone (4) has been obtained from a marine plant. from the methanol extract of calloseris sp., we have achieved the second isolation ...201526198236
in vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, lenzites quercina.in recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. the phytochemistry as well as antimicrobial activity of extracts obtained from lenzites quercina was investigated. the extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. the quantity of phytochemicals found in the extra ...201526604928
phylogenetic relationship of phosphate solubilizing bacteria according to 16s rrna genes.phosphate solubilizing bacteria (psb) can convert insoluble form of phosphorous to an available form. applications of psb as inoculants increase the phosphorus uptake by plant in the field. in this study, isolation and precise identification of psb were carried out in malaysian (serdang) oil palm field (university putra malaysia). identification and phylogenetic analysis of 8 better isolates were carried out by 16s rrna gene sequencing in which as a result five isolates belong to the beta subdiv ...201525632387
melanin-like pigment synthesis by soil bacillus weihenstephanensis isolates from northeastern poland.although melanin is known for protecting living organisms from harmful physical and chemical factors, its synthesis is rarely observed among endospore-forming bacillus cereus sensu lato. here, for the first time, we reported that psychrotolerant bacillus weihenstephanensis from northeastern poland can produce melanin-like pigment. we assessed physicochemical properties of the pigment and the mechanism of its synthesis in relation to b. weihenstephanensis genotypic and phenotypic characteristics. ...201525909751
pcr detection and identification of bacterial contaminants in ocular samples from post-operative endophthalmitis.bacterial endophthalmitis is a sight-threatening complication of ocular surgery which requires urgent medical consideration including comprehensive diagnosis. polymerase chain reaction (pcr) as a sensitive molecular method has been extensively used for detection of microbial species in clinical specimens.201526023576
ces locus embedded proteins control the non-ribosomal synthesis of the cereulide toxin in emetic bacillus cereus on multiple levels.the emetic toxin cereulide produced by bacillus cereus is synthesized by the modular enzyme complex ces that is encoded on a pxo1-like megaplasmid. to decipher the role of the genes adjacent to the structural genes cesa/cesb, coding for the non-ribosomal peptide synthetase (nrps), gene inactivation- and overexpression mutants of the emetic strain f4810/72 were constructed and their impact on cereulide biosynthesis was assessed. the hydrolase cesh turned out to be a part of the complex regulatory ...201526528255
bacillus cereus efflux protein bc3310 - a multidrug transporter of the unknown major facilitator family, umf-2.phylogenetic classification divides the major facilitator superfamily (mfs) into 82 families, including 25 families that are comprised of transporters with no characterized functions. this study describes functional data for bc3310 from bacillus cereus atcc 14579, a member of the "unknown major facilitator family-2" (umf-2). bc3310 was shown to be a multidrug efflux pump conferring resistance to ethidium bromide, sds and silver nitrate when heterologously expressed in escherichia coli dh5α δacrab. ...201526528249
three novel lantibiotics, ticins a1, a3, and a4, have extremely stable properties and are promising food biopreservatives.lantibiotics are antimicrobial peptides with potential applications as the next generation of antimicrobials in the food industry and/or the pharmaceutical industry. nisin has successfully been used as a food preservative for over 40 years, but its major drawback is its limited stability under neutral and alkaline ph conditions. to identify alternatives with better biochemical properties, we screened more than 100 strains of the bacillus cereus group. three novel lantibiotics, ticins a1 (4,062.9 ...201526231642
effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria.enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ...201526464607
the bacillus cereus group is an excellent reservoir of novel lanthipeptides.lantibiotics are ribosomally synthesized peptides that contain multiple posttranslational modifications. research on lantibiotics has increased recently, mainly due to their broad-spectrum antimicrobial activity, especially against some clinical gram-positive pathogens. many reports about various bacteriocins in the bacillus cereus group have been published, but few were about lantibiotics. in this study, we identified 101 putative lanthipeptide gene clusters from 77 out of 223 strains of this g ...201525548056
chemical composition and antimicrobial activity of hexane leaf extract of anisopus mannii (asclepiadaceae).the aim was to determine the chemical constituents and antimicrobial activity of the hexane leaf extract of anisopus mannii against a wide range of human pathogenic microorganisms.201526401399
bacteria associated with amblyomma cajennense tick eggs.ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ...201526537602
inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming lactobacillus isolate: a potential host for the expression of heterologous proteins.bacterial biofilms are a preferred mode of growth for many types of microorganisms in their natural environments. the ability of pathogens to integrate within a biofilm is pivotal to their survival. the possibility of biofilm formation in lactobacillus communities is also important in various industrial and medical settings. lactobacilli can eliminate the colonization of different pathogenic microorganisms. alternatively, new opportunities are now arising with the rapidly expanding potential of ...201526150120
methylatable signaling helix coordinated inhibitory receiver domain in sensor kinase modulates environmental stress response in bacillus cereus.σb, an alternative transcription factor, controls the response of the cell to a variety of environmental stresses in bacillus cereus. previously, we reported that rsbm negatively regulates σb through the methylation of rsbk, a hybrid sensor kinase, on a signaling helix (s-helix). however, rsbk comprises a c-terminal receiver (rec) domain whose function remains unclear. in this study, deletion of the c-terminal rec domain of rsbk resulted in high constitutive σb expression independent of environm ...201526379238
multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium.routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ...201526579100
bacillus cereus atcc 14579 rpon (sigma 54) is a pleiotropic regulator of growth, carbohydrate metabolism, motility, biofilm formation and toxin production.sigma 54 is a transcriptional regulator predicted to play a role in physical interaction of bacteria with their environment, including virulence and biofilm formation. in order to study the role of sigma 54 in bacillus cereus, a comparative transcriptome and phenotypic study was performed using b. cereus atcc 14579 wt, a markerless rpon deletion mutant, and its complemented strain. the mutant was impaired in many different cellular functions including low temperature and anaerobic growth, carboh ...201526241851
the biosynthesis of udp-d-quinac in bacillus cereus atcc 14579.n-acetylquinovosamine (2-acetamido-2,6-di-deoxy-d-glucose, quinac) is a relatively rare amino sugar residue found in glycans of few pathogenic gram-negative bacteria where it can play a role in infection. however, little is known about quinac-related polysaccharides in gram-positive bacteria. in a routine screen for bacillus glycan grown at defined medium, it was surprising to identify a quinac residue in polysaccharides isolated from this gram-positive bacterium. to gain insight into the biosyn ...201526207987
proteolytic activities expressed by gastrointestinal pathogens bacillus cereus, listeria monocytogenes and enterococcus faecium in different growth phases.bacterial proteases are implicated in protein quality control, biofilm formation or might have a direct function in pathogenesis by processing virulence factors or cleaving host factors. in recent years, knowledge of proteases expressed by gram-negative pathogens remarkably increased. however, investigation of proteases from gram-positive bacteria is rather rare, but required for the analysis of pathogenesis-relevant proteases. in this study, we extracted and detected proteases from the gastroin ...201526682199
antimicrobial and antiquorum-sensing studies. part 3: synthesis and biological evaluation of new series of [1,3,4]thiadiazoles and fused [1,3,4]thiadiazoles.new series of [1,3,4]thiadiazoles and fused [1,3,4]thiadiazoles were synthesized. the newly synthesized compounds were screened for their antibacterial activity against staphylococcus aureus, bacillus cereus, and escherichia coli. compounds 3b and 10a displayed the highest activity against e. coli with mic value of 78.125 μg/ml. in addition, compound 10a exhibited the highest activity against b. cereus with mic value of 156.25 μg/ml. the antifungal activity of these compounds was also tested aga ...201525773477
distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing dna dataset analysis.this study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. the 13 samples were sequenced using an illumina hi-seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. all samples were cul ...201525724392
antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated from polygonatum verticillatum rhizomes.this study was designed to assess the susceptibility of various microorganisms and inhibition on heat-induced protein denaturation against diosgenin and santonin, isolated from polygonatum verticillatum rhizomes. both diosgenin and santonin showed significant zone of inhibition when studied against various gram-positive (bacillus subtilis, bacillus cereus, staphylococcus aureus and staphylococcus epidermidis) and gram-negative bacteria (escherichia coli and salmonella typhi). in antifungal assay ...201525553804
synthesis, spectroscopic, anticancer, antibacterial and antifungal studies of ni(ii) and cu(ii) complexes with hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene].schiff's base ligand(l) hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene] and its metal complexes have been synthesized and characterized by elemental analysis, molar conductance, various spectroscopic techniques such as electronic, ir, (1)h nmr, mass, epr. molar conductance of complexes in dmf solution corresponds to non-electrolyte. complexes have general composition [m(l)2x2], where m=ni(ii) and cu(ii), x=cl(-), no3(-), ch3coo(-) and ½so4(2-). on the basis of above spectral studies, an ...201525087168
screening of microorganisms from deep-sea mud for antarctic krill (euphausia superba) fermentation and evaluation of the bioactive compounds.twelve kinds of strains were isolated from deep-sea mud which can use antarctic krill powder as the sole carbon/nitrogen source. these strains were identified by 16s rdna sequence analysis and grouped into eight different genera, including bacillus, shewanella, psychrobacter, klebsiella, macrococcus, aeromonas, acinetobacter, and saccharomyces. after fermentation of antarctic krill powder using these strains, bioactive compounds including total phenolics, free amino acids, and enzyme activities ...201525416479
[preliminary analyses on bacterial diversity and resistance in infection-related skin disorders].to explore the bacterial diversity and resistance in infection-related skin disorders.201526704163
microbiological stability of canned tuna produced in italy and in non-european countries.the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ...201527800383
antimicrobial activity of neo-clerodane diterpenoids isolated from lamiaceae species against pathogenic and food spoilage microorganisms.antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of scutellaria and salvia species (lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria aeromonas hydrophila, bacillus cereus, escherichia coli, listeria monocytogenes, proteus vulgaris, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella abony and staphylococcus aureus as well as against two yeast st ...201526749799
a cluster of bacillus cereus bacteremia cases among injection drug users.bacillus cereus is a ubiquitous spore-forming organism that is infrequently implicated in extraintestinal infections. the authors report three cases of b cereus bacteremia among injection drug users presenting within one month to an urban tertiary care hospital. treatment with intravenous vancomycin was successful in all three cases. while temporal association suggested an outbreak, molecular studies of patient isolates using pulsed-field gel electrophoresis did not suggest a common source. a re ...201526015795
bacteria present in comadia redtenbacheri larvae (lepidoptera: cossidae).the external and internal culturable bacterial community present in the larvae of comadia redtenbacheri hammerschmidt, an edible insect, was studied. characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16s rrna gene revealed the existence of paenibacillus sp., bacillus safensis, pseudomonas sp., bacillus pseudomycoides, corynebacterium variabile, enterococcus sp., gordonia sp., acinetobacter calcoaceticus, arthrobacter sp., micrococcus sp. ...201526336239
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
endophytic bacteria from pinellia ternata, a new source of purine alkaloids and bacterial manure.pinellia ternata (thunb.) berit., a perennial herb belonging to araceae, is one of the few medicinal plants to produce purine alkaloids. it is speculated that endophytic bacteria from p. ternata may produce guanosine or inosine. however, there is no report about endophytic bacteria in p. ternata.201525868618
[screening probiotic endophytic bacteria from medicinal plant flex cornuta and the phytopathogen-inhibiting effect].culturable endophytic bacteria were isolated from medicinal plant ilex cornuta by plate-spreading method, strains with strong inhibitory effect on phytopathogen were screened by confrontation culture and fermentation filtrate culture methods, and the morphological changes of phytopathogen hyphae treated with endophytic bacteria were examined by microscopy and micrograph. their phylogenetic relationships were determined by homology analysis of the 16s rdna sequences of pcr products and the taxono ...201526571677
characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin.minimal inhibitory concentration (mic) of chitosan lactate (chl) was tested against bacteria and phytopathogenic fungi. then, the structural, physicochemical and antimicrobial properties of films based on chl, oxidized potato starch (ops), and gelatin (gel) were investigated. with the exception of rhizopus nigricans, chl was effective against the target organisms. gram-positive bacteria (staphylococcus aureus and bacillus cereus) were more sensitive to chl than gram-negative bacteria (pectobacte ...201525841370
assessment of microbial contamination within working environments of different types of composting plants.the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ...201525947216
isolation & identification of bacteria for the treatment of brown crab (cancer pagurus) waste to produce chitinous material.to isolate bacteria from soil for microbial pretreatment of brown crab (cancer pagurus) shell waste and the production of chitin.201525644656
synthesis and in vitro antimicrobial evaluation of piperazine substituted quinazoline-based thiourea/thiazolidinone/chalcone hybrids.in frames of the search for new biological entities to fight against recent drug-resistant microbial strains, we report a library of quinazoline-based thiourea/4-thiazolidinone/chalcone hybrids. the newly synthesized compounds were studied for efficacy against several bacteria (staphylococcus aureus, bacillus cereus, pseudomonas aeruginosa, and klebsiella pneumoniae) and fungi (candida albicans and aspergillus clavatus) using the broth dilution technique. from the biological evaluation, (e)-3-(3 ...201526165131
diversity of food-borne bacillus volatile compounds and influence on fungal growth.to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition.201525989039
microwave assisted one-pot catalyst free green synthesis of new methyl-7-amino-4-oxo-5-phenyl-2-thioxo-2,3,4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates as potent in vitro antibacterial and antifungal activity.an efficiently simple protocol for the synthesis of methyl 7 amino-4-oxo-5-phenyl-2-thioxo-2, 3, 4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates via one-pot three component condensation pathway is established via microwave irradiation using varied benzaldehyde derivatives, methylcyanoacetate and thio-barbituric acid in water as a green solvent. a variety of functionalized substrates were found to react under this methodology due to its easy operability and offers several advantages like ...201526644932
[differential sensitivity of microorganisms to polyhexamethyleneguanidine].factors identified that affect the sensitivity of microorganisms to polyhexamethyleneguanidine (phmg). salts of phmg chloride, valerate, maleate, succinate was to use. test strains of esherichia coli, staphylococcus aureus, bacillus cereus, leptospira interrogans, paenibacillus larvae, mycobacterium bovis, m. avium, m. fortuitum, aspergillus niger and some strains of viruses are taken as objects of research. we have determined that the cytoplasm membrane phospholipids is main "target" for the po ...201526638480
composition and bioactivities of an (e)-β-farnesene chemotype of chamomile (matricaria chamomilla) essential oil from nepal.the essential oil of matricaria chamomilla, collected from nepal, was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the major components in nepalese chamomile oil were (e)-β-famesene (42.2%), α-bisabolol oxide a (22.3%), (e,e)-α-famesene (8.3%), cis-bicycloether (5.0%), α-bisabolol oxide b (4.5%), and α-bisabolone oxide a (4.0%). a cluster analysis based on the chemical compositions of 48 samples of chamomile oil reported in the literature has revealed seven ...201526434140
mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes.in the present study, silver nanoparticles (agnps) synthesized from aqueous leaves extract of malva crispa and their mode of interaction with food- and water-borne microbes were investigated. formation of agnps was conformed through uv-vis, fe-sem, eds, afm, and hr-tem analyses. further the concentration of silver (ag) in the reaction mixture was conformed through icp-ms analysis. different concentration of nanoparticles (1-3 mm) tested to know the inhibitory effect of bacterial pathogens such a ...201526178241
ieodoglucomide c and ieodoglycolipid, new glycolipids from a marine-derived bacterium bacillus licheniformis 09idym23.chemical examination of the ethyl acetate extract from the fermentation broth of the marine-derived bacterium bacillus licheniformis resulted in the isolation of two new glycolipids, ieodoglucomide c (1) and ieodoglycolipid (2). the structural characterization of 1 and 2 was achieved by extensive spectroscopic evidence, including 2d nmr experiments. a combination of chemical derivatization techniques followed by nmr studies, lc-ms data analysis and a literature review was deployed for the establ ...201525893812
secondary metabolites of seseli rigidum: chemical composition plus antioxidant, antimicrobial and cholinesterase inhibition activity.extracts of different polarity obtained from various plant parts (root, leaf, flower and fruit) of seseli rigidum were studied by different antioxidant assays: dpph and abts radical scavenging activity, by total reducing power method as well as via total content of flavonoids and polyphenols. essential oils of all plant parts showed weak antioxidant characteristics. the inhibitory concentration range of the tested extracts, against bacteria escherichia coli, pseudomonas aeruginosa, staphylococcu ...201525863020
detection of boron removal capacities of different microorganisms in wastewater and effective removal process.in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ...201526540546
diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in aloe vera.twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of aloe vera and molecularly characterized to 13 genera: pseudomonas, bacillus, enterobacter, pantoea, chryseobacterium, sphingobacterium, aeromonas, providencia, cedecea, klebsiella, cronobacter, macrococcus and shigella. the dominant genera include bacillus (20.7%), pseudomonas (20.7%) and enterobacter (13.8%). the crude and ethyl acetate fractions of the metabolites of six isolates, ...201526454221
chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants.crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ...201525910648
characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark).the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ...201525740801
microbial growth under supercritical co2.growth of microorganisms in environments containing co2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. thus, supercritical co2 (scco2) is generally regarded as a sterilizing agent. we report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (gcs) that are capable of growth in pressurized bioreactors containing scco2. analysis of 16s rrna genes from scco2 ...201525681188
identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp.the main goal of this work was the identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. that may be useful for typing purposes. these species include, among others, bacillus cereus, an important pathogenic species involved in food poisoning, and bacillus licheniformis, bacillus subtilis and bacillus pumilus, which are causative agents of food spoilage described as responsible for foodborne disease outbreaks. with this purpose in mind, 52 bacill ...201525475292
real-time pcr identification of unique bacillus anthracis sequences.bacillus anthracis is a spore-forming, gram-positive microorganism. it is a causative agent of anthrax, a highly infectious disease. it belongs to the "bacillus cereus group", which contains other closely related species, including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus weihenstephanensis, and bacillus pseudomycoides. b. anthracis naturally occurs in soil environments. the ba5345 genetic marker was used for highly specific detection of b. anthracis with taqman probe ...201526667574
spore germination.despite being resistant to a variety of environmental insults, the bacterial endospore can sense the presence of small molecules and respond by germinating, losing the specialized structures of the dormant spore, and resuming active metabolism, before outgrowing into vegetative cells. our current level of understanding of the spore germination process in bacilli and clostridia is reviewed, with particular emphasis on the germinant receptors characterized in bacillus subtilis, bacillus cereus, an ...201527337279
impacts of sporulation temperature, exposure to compost matrix and temperature on survival of bacillus cereus spores during livestock mortality composting.to investigate impact of sporulation and compost temperatures on feasibility of composting for disposal of carcasses contaminated with bacillus anthracis.201525580774
cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in bacillus cereus atcc 14579.loss of the cytochrome c maturation system in bacillus cereus results in increased transcription of the major enterotoxin genes nhe, hbl, and cytk and the virulence regulator plcr. increased virulence factor production occurs at 37°c under aerobic conditions, similar to previous findings in bacillus anthracis. unlike b. anthracis, much of the increased virulence gene expression can be attributed to loss of only c551, one of the two small c-type cytochromes. additional virulence factor expression ...201525422307
potentiality of eisenia fetida to degrade disposable paper cups-an ecofriendly solution to solid waste pollution.the aim of the present study was to subject the post-consumer waste, namely paper cups for vermicomposting along with cow dung in three different ratios for a period of 90-140 days employing eisenia fetida. the post-consumer wastes are a menace in many developing countries including india. this waste was provided as feed for earthworms and was converted to vermicompost. vermicompost prepared with paper cup waste was analyzed for their physicochemical properties. based on the physicochemical prop ...201525220767
mercuric reductase activity of multiple heavy metal-resistant lysinibacillus sphaericus g1.a culture was isolated from an industrial mercuric salt-contaminated soil, which could tolerate cd, co, zn, cr, and hg up to 190, 525, 350, 935, and 370 μm, respectively. the isolate was identified as lysinibacillus sphaericus by 16s rrna gene sequencing. it bioaccumulated cd, co, and zn, and reductively detoxified cr and hg. chromate reductase and mercuric reductase (mera) activities in the cell extract were 2.4 and 0.13 units mg(-1) protein, respectively. the study also describes designing of ...201524132860
antibacterial, antioxidant and cytotoxic studies of total saponin, alkaloid and sterols contents of decoction of joshanda: identification of components through thin layer chromatography.the current study was aimed to assess antibacterial, antioxidant and cytotoxic activity of total saponin, alkaloid and sterol contents of joshanda decoction followed by its constituent's analysis via thin layer chromatography (tlc). saponins and alkaloids showed prominent antibacterial activity against staphylococcus aureus, bacillus subtilis, bacillus cereus and klebsiella pneumoniae whereas sterols only against s. aureus. saponin and alkaloid contents of 97 and 108 µg/ml, respectively, showed ...201523235996
combined effect of cadmium, lead, and uv rays on bacillus cereus using comet assay and oxidative stress parameters.exposure to environmental chemicals and oxidative stress particularly at low dose levels may produce additive or synergistic interactions not seen in single component exposure. exposure to cadmium, lead, and ultraviolet rays occurs in many occupational settings, such as pigment and battery production, galvanization, and recycling of electric tools. however, little is known about interactions between heavy metals and ultraviolet rays. this study aimed to evaluate the interactions of ultraviolet r ...201523089956
comparison of antibacterial activity of ag nanoparticles synthesized from leaf extract of parthenium hystrophorus l in aqueous media and gentamicin sulphate: in-vitro.monodisperse silver (ag) nanoparticles were synthesized by using parthenium hystrophorus l leaf extract in aqueous media. the synthesized nanoparticles were characterized by using uv-vis spectrophotometer, x-ray diffracto-meter (xrd), transmission electron microscope (tem), and dynamics light scattering (dls). size-dependent antibacterial activities of ag nanoparticles were tested against gram negative pseudomonas aeruginosa and gram positive staphylococcus aureus. ag nanoparticles having 20 ± 2 ...201524111829
evaluation of haccp system implementation on the quality of mixed fresh-cut salad prepared in a university canteen: a case study.the increasing awareness that foods can represent vehicles for health risk factors has caused scientists and public authorities to multiply their efforts to reduce these risks to within acceptable limits. nevertheless, some challenging issues still remain unsolved and new ones have recently emerged, such as the increase in outbreaks of foodborne diseases originating from the consumption of meals at catering facilities. the study described in this article was aimed at evaluating the microbiologic ...201525619040
an insect gut environment reveals the induction of a new sugar-phosphate sensor system in bacillus cereus.bacteria survive under various conditions by sensing stimuli triggering specific adaptive physiological responses, which are often based on membrane-integrated sensors connected to a cytoplasmic regulator. recent studies reveal that mucus glycans may act as signal molecules for two-component systems involved in intestinal colonization. bacillus cereus, a human and insect opportunistic pathogen was used to identify bacterial factors expressed in an insect gut infection model. the screen revealed ...201524256737
effect of crude extracts of selected actinomycetes on biofilm formation of a. schindleri, m. aci, and b. cereus.actinomycetes are well known group of gram positive bacteria for their potential to produce antibiotics. this study sought to assess the ability of the selected actinomycetes to control biofilm forming bacteria isolated from different dental plaque samples. on the basis of morphological differences three out of ten different dental plaque bacterial isolates were selected for further study. these isolates were biochemically and genetically characterized and were identified as acinetobacter schinn ...201525138589
simultaneous efficient removal of high-strength ammonia nitrogen and chemical oxygen demand from landfill leachate by using an extremely high ammonia nitrogen-resistant strain.bioaugmentation is a promising technology for pollutant elimination from stressed environments, and it would provide an efficient way to solve challenges in traditional biotreatment of wastewater with high strength of ammonia nitrogen (nh4(+)-n). a high nh4(+)-n-resistant bacteria strain, identified as bacillus cereus (jlu bc), was domesticated and isolated from the bacteria consortium in landfill leachate. jlu bc could survive in 100 g/l nh4(+)-n environment, which indicated its extremely high ...201525196098
is atomic rearrangement of type iv pha synthases responsible for increased pha production?type iv pha synthase is a key enzyme responsible for catalyzing the formation of non-toxic, biocompatible, and biodegradable short-chain-length polyhydroxyalkanoates (scl-pha) under the growth-limiting conditions in the members of the genus bacillus.201525077376
functional characterization and phylogenetic analysis of acquired and intrinsic macrolide phosphotransferases in the bacillus cereus group.the bacillus cereus group is composed of gram-positive spore-forming bacteria of clinical and ecological importance. more than 200 b. cereus group isolates have been sequenced. however, there are few reports of b. cereus group antibiotic resistance genes. this study identified two functional classes of macrolide phosphotransferases (mphs) in the b. cereus group. cluster a mphs inactivate 14- and 15-membered macrolides while cluster b mphs inactivate 14-, 15- and 16-membered compounds. the genomi ...201525059531
characterization of a novel thermostable n-acylhomoserine lactonase from the thermophilic bacterium thermaerobacter marianensis.thermaerobacter marianensis is an extremely thermophilic bacterium, which was isolated from the mariana trench, with an optimal growth temperature of approximately 75 °c. n-acylhomoserine lactone (ahl) is a quorum-sensing signal molecule used by many gram-negative bacteria. here, we report the identification of an ahl-degrading gene homolog (designated aiit) in the genome of t. marianensis jcm 10246. aiit has 59.7%, 21.2%, and 11.2% identity to ahls from solibacillus silvestris, aiia from bacill ...201525529553
characteristics and phylogeny of bacillus cereus strains isolated from maari, a traditional west african food condiment.maari is a spontaneously fermented food condiment made from baobab tree seeds in west african countries. this type of product is considered to be safe, being consumed by millions of people on a daily basis. however, due to the spontaneous nature of the fermentation the human pathogen bacillus cereus occasionally occurs in maari. this study characterizes succession patterns and pathogenic potential of b. cereus isolated from the raw materials (ash, water from a drilled well (dw) and potash), seed ...201525528535
antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain.the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ...201525440998
probabilistic exposure assessment model to estimate aseptic-uht product failure rate.aseptic-ultra-high-temperature (uht) products are manufactured to be free of microorganisms capable of growing in the food at normal non-refrigerated conditions at which the food is likely to be held during manufacture, distribution and storage. two important phases within the process are widely recognised as critical in controlling microbial contamination: the sterilisation steps and the following aseptic steps. of the microbial hazards, the pathogen spore formers clostridium botulinum and baci ...201525440556
prevalence, genetic diversity, and antibiotic resistance of bacillus cereus isolated from korean fermented soybean products.bacillus cereus contamination is a major food safety problem for korean fermented soybean products, but few studies have assessed its potential to cause foodborne illness. the objectives of this study were to investigate the prevalence and characteristics of b. cereus isolated from korean fermented soybean products. b. cereus was detected in 110 of 162 (67.9%) samples. the highest b. cereus frequency was observed in deonjang (68 of 93 samples, 73.1%) and cheonggukjang (18 of 25, 72.0%); however, ...201525472031
the analysis of distribution of multidrug resistant pseudomonas and bacillus species from burn patients and burn ward environment.infections caused by multidrug resistant bacteria act as a risk factor for mortality in burns patients. so keeping in view the crucial importance of reliable therapeutic decisions of multidrug resistance bacteria and role of hospital environment in bacteria colonization, our study is based on the evaluation of distribution of pseudomonas sp. and bacillus sp. in burn patients and burn ward environment.201525468476
effect of packaging technology on microbiological and sensory quality of a cooked blood sausage, morcela de arroz, from monchique region of portugal.morcela de arroz (ma), a popular portuguese blood sausage, with high ph and water activity (aw), is traditionally commercialized without preservatives and unpacked. this study evaluated the best packaging solution to extend ma shelf life stored at 4±1°c for 44days: without packaging (wp), vacuum (vp) and modified atmosphere packaging (map) (80% co2; 20% n2). mesophilic (mtvc), psychrotrophic (ptvc), lactic acid bacteria (lab), pseudomonads, molds and yeasts, enterobacteriaceae, listeria monocyto ...201525462380
integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures.biological production of hydrogen (h2) and polyhydroxybutyrate (phb) from pea-shell slurry (pss) was investigated using defined mixed culture (mmc4, composed of enterobacter, proteus, bacillus spp.). under batch culture, 19.0lh2/kg of pss (total solid, ts, 2%w/v) was evolved. using effluent from the h2 producing stage, bacillus cereus egu43 could produce 12.4% (w/w) phb. dilutions of pss hydrolysate containing glucose (0.5%, w/v) resulted in 45-75lh2/kg ts fed and 19.1% (w/w) of phb content. und ...201525460994
aerobic biodegradation of odorous dimethyl disulfide in aqueous medium by isolated bacillus cereus gigan2 and identification of transformation intermediates.a novel, flagellated, rod-shape, gram-positive facultative aerobe, was isolated and identified as bacillus cereus gigan2. it can effectively remove model odorous organics dimethyl disulfide (dmds) in aqueous solution under aerobic conditions. initial concentration, ph value and temperature played important role in dmds biodegradation, and up to 100% of 10mgl(-1) of dmds could be removed within 96h under the optimum conditions (30°c, ph 7.0 and 200rpm) with a maximum biodegradation rate constant ...201525459868
antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of calotropis gigantea l.the present study aimed to synthesis silver nanoparticles (agnps) in a greener route using aqueous latex extract of calotropis gigantea l. toward biomedical applications. initially, synthesis of agnps was confirmed through uv-vis spectroscopy which shows the surface plasmonic resonance peak (spr) at 420 nm. fourier transform infrared spectroscopy (ftir) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. energy dispersi ...201525459618
an emetic bacillus cereus outbreak in a kindergarten: detection and quantification of critical levels of cereulide toxin.a bacillus cereus-related emetic outbreak was reported in a belgian kindergarten. high levels of emetic b. cereus (>1.5e+07 colony-forming units/g) were detected in the food leftovers, and the presence of an emetic strain was confirmed in feces. emetic toxin levels ranging up to 4.2 μg/g were also quantified in the leftovers by liquid chromatography coupled to tandem mass spectrometry (lc-ms(2)) analysis. those levels, although moderate in comparison with earlier published intoxications, provoke ...201525457101
brex is a novel phage resistance system widespread in microbial genomes.the perpetual arms race between bacteria and phage has resulted in the evolution of efficient resistance systems that protect bacteria from phage infection. such systems, which include the crispr-cas and restriction-modification systems, have proven to be invaluable in the biotechnology and dairy industries. here, we report on a six-gene cassette in bacillus cereus which, when integrated into the bacillus subtilis genome, confers resistance to a broad range of phages, including both virulent and ...201525452498
association of a zn(2+) containing metallo β-lactamase with the anticancer antibiotic mithramycin.pathogenic bacteria that are resistant to β-lactam antibiotics mostly utilize serine β-lactamases to degrade the antibiotics. current studies have shown that different subclasses of metallo β-lactamases (e[mbl]) are involved in the defense mechanism of drug resistant bacteria. here we report that the zn(2+) containing subclass b1 e[mbl] from bacillus cereus binds to a naturally occurring anti-cancer drug mithramycin (mtr). spectroscopic (cd and fluorescence) and isothermal titration calorimetry ...201525450021
establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in saccharomyces cerevisiae via β-alanine.microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global co2 emissions. 3-hydroxypropionic acid (3hp) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. with the objective of developing saccharomyces cerevisiae as an efficient cell factory for high-level production of 3hp, we identified the β-alanine biosynthetic route as the most economically attractive according to t ...201525447643
pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea.linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea.201525433250
production and purification of anti-bacterial biometabolite from wild-type lactobacillus, isolated from fermented bamboo shoot: future suggestions and a proposed system for secondary metabolite onsite recovery during continuous fermentation.wild-type lactobacillus isolated form khorisa, a fermented bamboo shoot product of assam, india were evaluated for production anti-bacterial secondary biometabolites, against staphylococcus aureus. submerged fermentation technique was used for the production of secondary anti-microbial biometabolite by a single wild-type lactobacillus strain, which tested positive for the release of anti-bacterial factor(s). crude cell-free supernatant was obtained, followed by extraction in water-immiscible sol ...201525432345
in vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (solanum tuberosum cv vitelotte noire) following simulated gastro-intestinal digestion.analyses of antioxidant and in vitro antimicrobial and anti-proliferative activities of anthocyanin-rich extracts from purple potatoes, solanum tuberosum l. cv vitelotte noire (solanaceae), were performed by simulating both a domestic cooking process and human digestion. extracts of crude and cooked purple potato did not exhibit antimicrobial activity against the tester strains: bacillus cereus, escherichia coli and pseudomonas aeruginosa. the behaviour changed after the simulated gastrointestin ...201525420792
infection of cardiovascular implantable electronic devices: detection with sonication, swab cultures, and blood cultures.the number of patients with cardiovascular implantable electronic devices (cieds) is increasing. the purpose of this study was to compare three methods (sonication, swab cultures, and blood cultures) to detect bacteria in infections of cieds.201525377386
chemical composition and antimicrobial activity of polish herbhoneys.the present study focuses on samples of polish herbhoneys (hhs), their chemical composition and antimicrobial activity. a gas chromatography-mass spectrometry (gc-ms) method was used to analyse eight samples of herbal honeys and three samples of nectar honeys. their antimicrobial activities were tested on selected gram-positive (bacillus cereus, staphylococcus aureus, staphylococcus schleiferi) and gram-negative (escherichia coli) bacteria, as well as on pathogenic fungi candida albicans. ether ...201525308646
lifesaving liver transplantation for multi-organ failure caused by bacillus cereus food poisoning.bacillus cereus is a spore-forming, gram-positive bacterium that causes food poisoning presenting with either emesis or diarrhea. diarrhea is caused by proteinaceous enterotoxin complexes, mainly hemolysin bl, non-hemolytic enterotoxin (nhe), and cytotoxin k. in contrast, emesis is caused by the ingestion of the depsipeptide toxin cereulide, which is produced in b. cereus contaminated food, particularly in pasta or rice. in general, the illness is mild and self-limiting. however, due to cereulid ...201525323120
isolation and characterization of a furfural-degrading bacterium bacillus cereus sp. strain ds1.furfural was found to be the main organic pollutant in the wastewater coming from the diosgenin factory. this substance is derived from acidic pentosan in dioscorea zingiberensis and is also found in a variety of agricultural byproducts, including corncobs, oat, wheat bran, and sawdust. it is regarded as a toxicant and an inhibitor to the growth of microorganism in both sewage disposal and biological fermentation. a furfural-degrading strain (ds1) was isolated from activated sludge of wastewater ...201525274411
bioaugmentation of a sequencing batch biofilm reactor with comamonas testosteroni and bacillus cereus and their impact on reactor bacterial communities.the immobilization of microorganisms is essential for efficient bioaugmentation systems. the performance of bacillus cereus g5 as biofilm-forming bacteria and comamonas testosteroni a3 a 3,5 dinitrobenzoic acid (dnb)-degrading strain] in laboratory-scale sequencing batch biofilm reactors (sbbrs) treating dnb synthetic wastewater has been examined. the microbial diversity in the reactors was also explored. the reactor r3 inoculated with b. cereus g5 and c. testosteroni a3 together not only improv ...201525257599
development of biopolymer nanocomposite for silver nanoparticles and ciprofloxacin controlled release.screening of biopolymeric gel beads containing silver nanoparticles (ag-nps) stabilized in guar gum alkyl amine (ggaa) and ciprofloxacin (cip) was carried out in order to obtain a novel nanocomposite with controlled release profile of both antimicrobians. the selected matrix composed of alginate/high methoxyl pectin (hmp)/ggaa (4:4:1) was able to co-incorporate ag-nps and cip with encapsulation efficiency higher than 70%. sem images revealed good cohesivity and compatibility between the biopolym ...201525256547
structural data and biological properties of almond gum oligosaccharide: application to beef meat preservation.enzymatic hydrolysis of almond gum generates low molecular weight oligosaccharides (oag) with a yield of 33.5%. the generated oligosaccharides were purified and identified. oag analyses show that the most prominent residues were galactose and arabinose with traces of xylose, rhamnose, glucose and mannose. the glycosyl linkage positions were analyzed using gas chromatography-mass spectrometry showing a main chain composed of galactose units [ → 3)-gal-(1 → ] branched mainly with arabinose residue ...201525195541
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