Publications

experimentally infected human body lice (pediculus humanus humanus) as vectors of rickettsia rickettsii and rickettsia conorii in a rabbit model.	the human body louse, the natural vector of rickettsia prowazekii, is able to experimentally transmit the normally flea-borne rickettsia r. typhi, suggesting that the relationships between the body louse and rickettsiae are not specific. we used our experimental infection model to test the ability of body lice to transmit two prevalent tick-borne rickettsiae. each of two rabbits was made bacteremic by injecting intravenously 2 x 10(6) plaque-forming units of either r. rickettsii or r. conorii. f ...	2006	16606977
rocky mountain spotted fever.	rocky mountain spotted fever (rmsf) is an unusual but important dermatological condition to identify without hesitation. the classic triad of headache, fever, and a rash that begins on the extremities and travels proximally to involve the trunk is found in a majority of patients. the cutaneous centripetal pattern is a result of cell to cell migration by the causative organism rickettsia rickettsii. such individuals should receive prompt antimicrobial therapy and supportive care to avoid serious ...	2006	16643138
fatal human infection with rickettsia rickettsii, yucatán, mexico.	the first fatal rickettsia rickettsii infection was diagnosed in the southwest of mexico. the patient had fever, erythematous rash, abdominal pain, and severe central nervous system involvement with convulsive crisis. the diagnosis of r. rickettsii infection was established by immunohistochemistry and specific polymerase chain reaction.	2006	16704818
lymph node hemophagocytosis in rickettsial diseases: a pathogenetic role for cd8 t lymphocytes in human monocytic ehrlichiosis (hme)?	human monocytic ehrlichiosis (hme) and rocky mountain spotted fever (rmsf) are caused by ehrlichia chaffeensis and rickettsia rickettsii, respectively. the pathogenesis of rmsf relates to rickettsia-mediated vascular injury, but it is unclear in hme.	2006	16859547
infection of human endothelial cells with spotted fever group rickettsiae stimulates cyclooxygenase 2 expression and release of vasoactive prostaglandins.	rickettsiae, a diverse group of obligately intracellular gram-negative bacteria, include etiologic agents of the spotted fever and typhus groups of diseases. rocky mountain spotted fever and boutonneuse fever, due to rickettsia rickettsii and r. conorii, respectively, are characterized by widespread infection of the vascular endothelium, microvascular injury, and vasculitis. cultured human endothelial cells (ec) are highly susceptible to infection and respond by altering the expression of adhesi ...	2006	16926398
tick-borne rickettsial pathogens in ticks and small mammals in korea.	in order to investigate the prevalence of tick-borne infectious agents among ticks, ticks comprising five species from two genera (hemaphysalis spp. and ixodes spp.) were screened using molecular techniques. ticks (3,135) were collected from small wild-caught mammals or by dragging/flagging in the republic of korea (rok) and were pooled into a total of 1,638 samples (1 to 27 ticks per pool). from the 1,638 tick samples, species-specific fragments of anaplasma phagocytophilum (1 sample), anaplasm ...	2006	16957192
myocardial involvement in rocky mountain spotted fever: a case report and review.	rocky mountain spotted fever (rmsf), caused by rickettia rickettsii, is a serious tickborne illness that is endemic in the southeastern united states. although it is most commonly known as a cause of fever and rash, it can have systemic manifestations. the myocardium may rarely be involved, with symptoms that can mimic those of acute coronary syndromes. this report describes a case of serologically proven rmsf causing symptomatic myocarditis, manifested by chest pain, elevated cardiac enzyme lev ...	2006	17031247
brazilian spotted fever: a case series from an endemic area in southeastern brazil: epidemiological aspects.	brazilian spotted fever (bsf) is the most important tick-borne disease in brazil and is caused by rickettsia rickettsii and transmitted by the ixodid tick amblyomma cajennense, its main vector. we present epidemiologic aspects of a case series of patients admitted to the hospital das clínicas da unicamp from 1985 to 2003 with a confirmed diagnosis of bsf either by a fourfold rise in indirect immunofluorescence (ifa) titers of igg antibodies reactive with r. rickettsii or isolation of r. ricketts ...	2006	17114702
fatal case of brazilian spotted fever confirmed by immunohistochemical staining and sequencing methods on fixed tissues.	the authors describe the first characterization of rickettsia rickettsii in a fatal case occurring in rio de janeiro state, brazil.	2006	17114718
detection of rickettsia rickettsii and rickettsia sp. in blood clots in 24 patients from different municipalities of the state of sao paulo, brazil.	the authors detected rickettsia genus organisms using shell vial and polymerase chain reaction (pcr)/sequencing analysis in blood clots in patients suspected of having brazilian spotted fever (bsf). dna was detected using pcr with three sets of primers to access the glta, ompa, and ompb genes. sequence analysis was carried out using an automatic sequencer with bioedit software. seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. to date ...	2006	17114719
a rickettsial mixed infection in a dermacentor variabilis tick from ohio.	we present the first report of superinfection in a dermacentor variabilis tick from nature. the single tick, collected in ohio, was found infected with rickettsia belli, r. nontanensis, and r. rickettsii.	2006	17114734
rocky mountain spotted fever in arizona: documentation of heavy environmental infestations of rhipicephalus sanguineus at an endemic site.	a recent epidemiologic investigation identified 16 cases and 2 deaths from rocky mountain spotted fever (rmsf) in two eastern arizona communities. prevalence studies were conducted by collecting free-living ticks (acari: ixodidae) from the home sites of rmsf patients and from other home sites within the community. dry ice traps and flagging confirmed heavy infestations at many of the home sites. only rhipicephalus sanguineus ticks were identified and all developmental stages were detected. it is ...	2006	17114735
molecular characterization of rickettsia rickettsii infecting dogs and people in north carolina.	rocky mountain spotted fever (rmst) is an important cause of morbidity and mortality in people and dogs in the united states. disease manifestations are strikingly similar in both species, and illness in dogs can precede illness in people. r. rickettsii has been identified as a select agent by the cdc as a category c priority pathogen by the national institute of allergic and infectious diseases because it is amenable to use as a bioterror agent. the clinical and temporal relationship of natural ...	2006	17114748
spotted fever group rickettsial infection in dogs from eastern arizona: how long has it been there?	a serosurvey of free-roaming dogs for antibodies to spotted fever group rickettsiae was conducted using archival samples that had been collected in the white mountain region of eastern arizona during a plague study in 1996. immunoglobulin g antibodies to rickettsia rickettsii (5.1%) and to r. rhipicephali (3.6%) were demonstrated, and no cross-reactive samples were identified. this study indicates that r. rickettsii was likely present in the dog populations in this area prior to the recognition ...	2006	17114769
isolation of rickettsia rickettsii and rickettsia bellii in cell culture from the tick amblyomma aureolatum in brazil.	brazilian spotted fever (bsf) is a highly lethal disease caused by rickettsia rickettsii. in the present study, rickettsial infection was evaluated in 669 amblyomma aureolatum adult ticks collected from naturally infested dogs in taiaçupeba, a bsf-endemic area in the state of são paulo. ten (1.49%) ticks were infected with rickettsia bellii, and 6 (0.89%) ticks were infected with r. rickettsii. both rickettsia species were isolated and established in vero cell cultures. the rickettsia isolates w ...	2006	17114770
molecular typing of novel rickettsia rickettsii isolates from arizona.	seven isolates of rickettsia rickettsii were obtained from a skin biopsy, two whole-blood specimens, and from rhipicephalus sanguineus ticks from eastern arizona. molecular typing of seven isolates of r. rickettsii and dna samples from two other rh. sanguineus ticks infected with r. rickettsii was conducted by pcr and dna sequencing of rompa and 12 variable-number tandem repeat regions (vntrs). all dna specimens from arizona were identical to each other and to reference human and dermacentor and ...	2006	17114781
[study on the development of a real-time quantitative polymerase chain reaction assay to detect rickettsia].	to develop a real-time quantitative polymerase chain reaction(pcr) assay for detecting rickettsia rickettsii.	2006	17152516
dopamine agonists and pituitary tumor shrinkage.	the primary aim of this review has been to clarify the tumor shrinking effects of dopamine agonists on pituitary macroadenomas of different cell types. shrinkage is most dramatic for macroprolactinomas and is due to cell size reduction. seventy-nine percent of 271 definite macroprolactinomas were reduced in size by at least 25%, and 89% shrank to some degree. most shrinkage occurs during the first 3 months of treatment, although in a minority shrinkage is delayed. dopamine agonist resistance dur ...	2006	1352243
[false acute abdomen in spontaneous rupture of the rectus abdominis muscle].		2006	1831872
[false acute abdomen in spontaneous rupture of the rectus abdominis muscle].		2006	1831872
[perverse interactions].		2006	1594856
[new trends in clinical neurophysiology of the spinal cord (review of the literature)].		2006	1665637
myocardial entrapment by lymphoma: a cause of reversible segmental left ventricular dysfunction.		2006	1636600
in situ activation of syngeneic tumour-specific cytotoxic t lymphocytes: intra-pinna immunization followed by restimulation in the peritoneal cavity.	tumour-specific cytotoxic t lymphocytes (ctl) are usually obtained after immunization in vivo and restimulation of immune cells in vitro. we here describe the generation of syngeneic tumour-specific ctl within no more than 9 days by priming and restimulation in vivo. this is achieved only if the correct sites are used both for primary immunization (ear pinna) and for restimulation (peritoneal cavity). the kinetics of immune t cell induction and of the secondary response in vivo will be reported. ...	2006	1868488
in situ activation of syngeneic tumour-specific cytotoxic t lymphocytes: intra-pinna immunization followed by restimulation in the peritoneal cavity.	tumour-specific cytotoxic t lymphocytes (ctl) are usually obtained after immunization in vivo and restimulation of immune cells in vitro. we here describe the generation of syngeneic tumour-specific ctl within no more than 9 days by priming and restimulation in vivo. this is achieved only if the correct sites are used both for primary immunization (ear pinna) and for restimulation (peritoneal cavity). the kinetics of immune t cell induction and of the secondary response in vivo will be reported. ...	2006	1868488
[why do physicians and patients ever more often resort to practitioners of alternative medicine: the healer and the quack?].		2006	1563606
nutrition and head start.		2006	1458961
epidemiology of respiratory hazards: recent advances.	a bibliographical review of epidemiological studies published over the last five years has led us to individualize the themes that have been the subject of most recent publications (table i). these research themes are not new, which shows a certain continuity in epidemiological research on the respiratory risks of occupational origin. few studies are devoted to the identification of unrecognized respiratory risks; nevertheless, the methodology used, the indicators of effects studies contribute t ...	2006	1626104
the role of an adhesive (histoacryl) in tendon repair.	two in vitro experiments have been done comparing various techniques of tendon repair. these include repairs using an adhesive, 2-butyl cyanoacrylate (histoacryl), and repairs by the modified kessler technique, using 4/0 braided polyester and 4/0 stainless steel. mechanical analysis has shown that by replacing the circumferential suture of a modified kessler tendon repair with histoacryl, the tensile strength of the repair and the force needed to produce a gap can be increased by 30% to 40%. thi ...	2006	1479248
[torsion of the colonic epiploic appendages].	operations were carried out on 21 patients with torsion of epiploic appendages of the colon (sigmoid 19, ascending 1, hepatic flexure 1). this pathologic condition has no specific manifestations and occurs under the mask of other emergencies. the suggested symptom of "pain displacement" in combination with other signs allowed the correct diagnosis to be established before the operation in two thirds of patients. patients with torsion of epiploic appendages must be subjected to an emergency opera ...	2006	1388218
health status of the temporarily homeless population and residents of north west thames region.	to survey the health status of the temporarily homeless population of north west thames region and make comparisons with regional residents.	2006	1392918
percutaneous mitral balloon valvotomy.		2006	1538723
[surgical reperfusion and myocardial revascularization in patients with cardiogenic shock].		2006	1800823
[treatment of parkinsonism].		2005	1265907
clinical evaluation of tumor scanning with 169-yb-citrate.	169-yb-citrate was used as the tumor-scanning agent in 360 patients with malignant tumors and in 55 others with benign lesions and the results compared with 67-ga-citrate studies in other series. in malignant tumors, the overall positive detection rate for ytterbium was 65.3%; in benign lesions, the false-positive rate was 29.1%. good results were obtained in the extremities, head and neck, and pelvic area. of all tumors visualized, squamous cell carcinoma was detected most effectively (77.5%). ...	2005	1153741
[laryngeal suspension microsurgery].		2005	1230948
glutaric aciduria type i: unusual biochemical presentation.	we describe a patient with glutaryl-coenzyme a dehydrogenase deficiency, demonstrated by a residual enzyme activity of only 1% in cultured fibroblasts. although the clinical presentation was typical of glutaric aciduria type i, the urine concentrations of glutaric, glutaconic, and 3-hydroxyglutaric acids remained normal, even during episodes of clinical decompensation. an increased free glutarate level was demonstrated only in cerebrospinal fluid.	2005	1625098
experimental evidence of ischemic thresholds and functional recovery.	impaired blood flow below certain critical levels and an insufficient supply of energy-rich substrates cause failure of neuronal function, triggering biochemical disturbances that eventually lead to ischemic cell damage.	2005	1440719
the interaction between legalization of abortion and contraception in denmark.	trends in fertility, abortion, and contraceptive practice in denmark were analyzed, using previously compiled official statistics; the conclusion was drawn that easy access to abortion may contribute toward a decline in contraceptive practice depending on the level of contraceptive practice in the population and on the degree of confidence the population has in available contraceptive methods. in october 1973 denmark passed a law permitting women to obtain free abortion on demand. the number of ...	2005	538953
[morbidity with temporary loss of work capacity in the workers of the belmeken-sestrimo hydroelectric cascade].	the present work presents the results from the analysis on the morbidity with temporary disability from cascade "belmeken-sestrimo" (n = 94, men 73, women 21). by means of personnel method are studied the cases of morbidity with temporary disability and defined the basic and enlarged number of indices, analysed in sex, age, length of service, nosological structure and dynamics for a period of 3 years (1987-1989) for the 3 sites of hydroelectric cascade "belmeken-sestrimo", water-power station "m ...	2005	1364540
serologic survey of select infectious diseases in coyotes and raccoons in nebraska.	to obtain data about select zoonotic and other infectious diseases in free-ranging predators in five ecoregions in nebraska, sera were collected from 67 coyotes (canis latrans) and 63 raccoons (procyon lotor) from november 2002 through january 2003. for coyotes, antibodies were detected against canine distemper virus (cdv, 61%), francisella tularensis (32%), rickettsia rickettsi (13%), and flaviviruses (48%). none of the coyote sera had antibodies to borrelia burgdorferi, brucella canis, or six ...	2005	16456169
high-throughput molecular testing of ticks using a liquid-handling robot.	to meet the need for high-throughput sample testing, dna extraction kits based on the 96-well plate format have been developed for use with blood and tissue samples. these methods have not been applied to dna extractions from ticks. to meet this need, we developed a high-throughput method for dna extraction and polymerase chain reaction (pcr) testing of tick samples. a liquid-handling robot was used to extract dna in a 96-well binding column plate with vacuum manifold. the quantity, purity, and ...	2005	16465749
nf-kappab activation during rickettsia rickettsii infection of endothelial cells involves the activation of catalytic ikappab kinases ikkalpha and ikkbeta and phosphorylation-proteolysis of the inhibitor protein ikappabalpha.	rocky mountain spotted fever, a systemic tick-borne illness caused by the obligate intracellular bacterium rickettsia rickettsii, is associated with widespread infection of the vascular endothelium. r. rickettsii infection induces a biphasic pattern of the nuclear factor-kappab (nf-kappab) activation in cultured human endothelial cells (ecs), characterized by an early transient phase at 3 h and a late sustained phase evident at 18 to 24 h. to elucidate the underlying mechanisms, we investigated ...	2005	15618150
functional analysis of seca homologues from rickettsiae.	the molecular basis of protein secretion that underlines rickettsial pathogenesis remains unknown. this paper reports the molecular and functional analysis of the putative seca gene, an essential component of the sec-dependent protein secretion pathway, from rickettsia rickettsii and rickettsia typhi, the aetiological agents of rocky mountain spotted fever and murine typhus, respectively. the sequence analysis of the cloned seca genes from r. rickettsii and r. typhi show orfs of 2721 and 2718 nt ...	2005	15699207
rickettsial infection in animals and brazilian spotted fever endemicity.	we compared the rickettsial infection status of amblyomma cajennense ticks, humans, dogs, and horses in both brazilian spotted fever (bsf)-endemic and -nonendemic areas in the state of sao paulo, brazil. most of the horses and few dogs from bsf-endemic areas had serologic titers against rickettsia rickettsii antigens. in contrast, no dogs or horses from bsf-nonendemic areas had serologic titers against r. rickettsii antigens, although they were continually exposed to a. cajennense ticks. all hum ...	2005	15752445
changes in the adherens junctions of human endothelial cells infected with spotted fever group rickettsiae.	rickettsiae of the spotted fever group are obligately intracellular bacteria that primarily infect the vascular endothelium, invade adjacent cells propelled by actin polymerization, and cause severe systemic diseases. endothelial dysfunction and vascular leakage develop as a consequence; this effect is the pathophysiological mechanism that explains most clinical manifestations. here we report that rickettsial infection of cultured primary human endothelial cells is associated with the formation ...	2005	15778845
[rocky mountain spotted fever in an american tourist].	in a 28-year-old male american tourist who presented in the hospital with fever, cold shivers, headache, nausea, myalgia and arthralgia, rocky mountain spotted fever was suspected, partly because he came from an endemic region (the state of georgia). the patient was treated with doxycycline, 100 mg b.i.d.; 9 days after the first appearance of the symptoms, the diagnosis was confirmed by the report of a positive antibody titre against rickettsia rickettsii. the patient did not have exanthema. he ...	2005	15835630
central nervous system dysfunction associated with rocky mountain spotted fever infection in five dogs.	five dogs from the northeastern united states were presented with clinical signs of neurological disease associated with rocky mountain spotted fever (rmsf) infection. four of the five dogs had vestibular system dysfunction. other neurological signs included paresis, tremors, and changes in mentation. all of the dogs had an elevated indirect fluorescent antibody titer or a positive semiquantitative enzyme screening immunoassay titer for rickettsia rickettsii at the time of presentation. although ...	2005	15995164
rocky mountain spotted fever--changing ecology and persisting virulence.		2005	16093463
rocky mountain spotted fever from an unexpected tick vector in arizona.	rocky mountain spotted fever is a life-threatening, tick-borne disease caused by rickettsia rickettsii. this disease is rarely reported in arizona, and the principal vectors, dermacentor species ticks, are uncommon in the state. from 2002 through 2004, a focus of rocky mountain spotted fever was investigated in rural eastern arizona.	2005	16093467
detection of brazilian spotted fever infection by polymerase chain reaction in a patient from the state of são paulo.	brazilian spotted fever (bsf) cases have been increasing in the state of são paulo but no genomic information about local rickettsia isolated from humans has been well documented. we recovered spotted-fever group rickettsiae from a sample of patient blood cultured in vero cells using the shell vial technique. rickettsial dna fragments (glta, ompa, and, ompb genes) were detected, and analysis of the ompb gene base sequences showed identity with the rickettsia rickettsii ompb sequence available in ...	2005	16113868
expression and secretion of chemotactic cytokines il-8 and mcp-1 by human endothelial cells after rickettsia rickettsii infection: regulation by nuclear transcription factor nf-kappab.	infection of endothelial cells (ec) with rickettsia rickettsii results in rocky mountain spotted fever, an acute illness characterized by systemic inflammation. interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) are important chemokines for activating neutrophils and monocytes, respectively, and recruiting these circulating immune cells to the sites of inflammation. in this study, we have measured the expression and secretion of these chemokines during r. rickettsii infection of ...	2005	16128401
activation of p38 stress-activated protein kinase during rickettsia rickettsii infection of human endothelial cells: role in the induction of chemokine response.	rickettsia rickettsii, a gram-negative and obligate intracellular bacterium, preferentially infects the vascular endothelium during human infections leading to inflammation and dysfunction. the aim of this study was to determine whether r. rickettsii infection of endothelial cells (ec) activates p38 and/or c-jun n-terminal kinases (jnk) mitogen-activated protein (map) kinase, key regulatory proteins that control the response to inflammatory stimuli. we show that infection of cultured human ec re ...	2005	16153249
cardiac involvement in a patient with clinical and serological evidence of african tick-bite fever.	myocarditis and pericarditis are rare complications of rickettsiosis, usually associated with rickettsia rickettsii and r. conorii. african tick-bite fever (atbf) is generally considered as a benign disease and no cases of myocardial involvement due to rickettsia africae, the agent of atbf, have yet been described.	2005	16242016
evaluation of a western blot method for the detection of yersinia antibodies: evidence of serological cross-reactivity between yersinia outer membrane proteins and borrelia burgdorferi.	yersinia enterocolitica and yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. we evaluated a western blot assay which uses yersinia outer membrane proteins as antigens for the detection of yersinia antibodies as a replacement for the complement fixation (cf) assay. clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the cf assay, western blot assay, and enzyme-linked immuno ...	2005	16275939
a volumetric assessment of pollen levels in the air of ann arbor, michigan.		2005	1196148
autosomal recessive inheritance of nager acrofacial dysostosis.	nager acrofacial dysostosis is a variant of mandibulofacial dysostosis with severe micrognathia, malar hypoplasia, and radial limb defects. most cases are sporadic, but autosomal recessive inheritance has been suggested. a family is reported in which two sibs are affected by this syndrome, presenting further evidence for autosomal recessive inheritance. the recognition of this syndrome as a distinct entity has important implications. after the birth of a child with orofacial malformations sugges ...	2005	3367347
[immunotherapeutic approach in tuberculosis: thymus hormones].		2005	1287758
toxoplasmosis after splenectomy in sickle cell disease. report of two cases with recurrence in one child two years following antitoxoplasmic therapy.		2005	1253508
[the metastic development of breast carcinoma after surgical treatment of primary tumor. iv].		2005	1143694
[lymphocyte populations in hbag-positive acute hepatitis].		2005	1087387
milk carbohydrates of marsupials. ii. quantitative and qualitative changes in milk carbohydrates during lactation in the tammar wallaby (macropus eugenii).	milk was collected at various stages of lactation from a group of tammar wallabies, m. eugenii, in which parturition had been synchronized. the milk carbohydrate was determined by a phenol-sulfuric acid method which had been modified to give equal colour yields for galactose and glucose. the mean carbohydrate content increased gradually during the first 6 months of lactation to a peak of 13 g hexose/100 ml of milk, but then fell rapidly to much lower values, over the following 2 months. througho ...	2005	549552
[clinico-immunologic shifts in myocardial infarct].		2005	1274315
[the problems of cardiovascular pathology in adolescents].		2005	3320454
a sporadic case of legionnaires' disease in belgium.		2004	525201
[a case report of portal-systemic encephalopathy with normal portal vein pressure and non-cirrhosis of the liver].		2004	3320417
[the control of renin secretion: experimental data and clinical implications].		2004	356832
rickettsia species infecting amblyomma cooperi ticks from an area in the state of são paulo, brazil, where brazilian spotted fever is endemic.	owing to the potential role of the tick amblyomma cooperi in the enzootic cycle of rickettsia rickettsii, the etiologic agent of brazilian spotted fever (bsf), this study evaluated infection by rickettsia species in a. cooperi ticks collected from an area in brazil where bsf is endemic. among a total of 40 a. cooperi adult ticks collected in an area of bsf endemicity in the state of são paulo, pcr analysis detected dna of rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positiv ...	2004	14715737
antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens.	the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ...	2004	14765731
rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the united states.	ticks, including many that bite humans, are hosts to several obligate intracellular bacteria in the spotted fever group (sfg) of the genus rickettsia. only rickettsia rickettsii, the agent of rocky mountain spotted fever, has been definitively associated with disease in humans in the united states. herein we describe disease in a human caused by rickettsia parkeri, an sfg rickettsia first identified >60 years ago in gulf coast ticks (amblyomma maculatum) collected from the southern united states ...	2004	14999622
nf-kappab activation suppresses host cell apoptosis during rickettsia rickettsii infection via regulatory effects on intracellular localization or levels of apoptogenic and anti-apoptotic proteins.	rickettsia rickettsii, a gram-negative and obligate intracellular bacterium, is the causative agent of rocky mountain spotted fever. in human infections, the primary target of r. rickettsii infection is vascular endothelium. our laboratory has shown that activation of nuclear transcription factor-kappa b (nf-kappab) during r. rickettsii infection of cultured human endothelial cells protects against apoptosis by preventing the activation of apical caspases-8 and -9, and the effector caspase-3. to ...	2004	15135541
fatal cases of rocky mountain spotted fever in family clusters--three states, 2003.	rocky mountain spotted fever (rmsf), a tickborne infection caused by rickettsia rickettsii and characterized by a rash, has a case-fatality rate as high as 30% in certain untreated patients. even with treatment, hospitalization rates of 72% and case-fatality rates of 4% have been reported. this report summarizes the clinical course of three fatal cases of rmsf in children and related illness in family members during the summer of 2003. these cases underscore the importance of 1) prompt diagnosis ...	2004	15152183
rocky mountain spotted fever at koair children's hospital, 1990-2002.	the reported average annual incidence of rocky mountain spotted fever (rmsf) in kentucky is less than 5 per million population, although seroprevalence studies suggest that exposure to rickettsia riskettsii, the causative agent, is relatively common among children. the experience with rmsf at kosair children's hospital over a 12-year period was reviewed. fifteen cases were identified (5 boys and 10 girls). illness onset ranged from april to october, and 4 patients resided in jefferson county. th ...	2004	15152446
a rickettsia wasp-like protein activates the arp2/3 complex and mediates actin-based motility.	spotted fever group rickettsia are obligate intracellular pathogens that exploit the host cell actin cytoskeleton to promote motility and cell-to-cell spread. although other pathogens such as listeria monocytogenes use an arp2/3 complex-dependent nucleation mechanism to generate comet tails consisting of y-branched filament arrays, rickettsia polymerize tails consisting of unbranched filaments by a previously unknown mechanism. we identified genes in several rickettsia species encoding proteins ...	2004	15236643
prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a brazilian spotted fever-endemic area in the state of são paulo, brazil: serologic evidence for infection by rickettsia rickettsii and another spotted fever group rickettsia.	in serum samples obtained from all the healthy humans, horses, dogs, and donkeys present on three farms in the pedreira municipality, an endemic area for brazilian spotted fever, an indirect immunofluorescence assay (ifa) detected antibodies against rickettsia rickettsii in 17 (77.3%) horses, 5 (31.3%) dogs (titers ranging from 64 to 4,048), and none of 4 donkeys or 50 humans. five canine and eight equine sera with high antibody titers to r. rickettsii were also tested by ifa against r. bellii, ...	2004	15238696
bartonella henselae igg antibodies are prevalent in dogs from southeastern usa.	in contrast to the large body of literature regarding bartonella henselae in humans and cats, there is little information about b. henselae as an infectious agent in dogs. due to the paucity of information regarding the b. henselae serology in dogs, we performed a cross-sectional serosurvey using b. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern usa. ninety-nine sera were collected from clinically healthy dogs. three hundred and one s ...	2004	15369661
rickettsial vaccines: the old and the new.	in the past century vaccine development for prevention of rickettsial diseases has been prolific. however, in the past 20 years no new rickettsial vaccine has been manufactured and there are currently no new or old rickettsial vaccines licensed. early rickettsial vaccines were difficult, expensive and very hazardous to produce. molecular biology techniques of today are currently being used to develop new rickettsial vaccines that are standardized, inexpensive, nonhazardous and efficacious.	2004	15485334
transfusion-transmitted tick-borne infections: a cornucopia of threats.	over the past several decades, the frequency of contact between humans and ticks has increased dramatically. concomitantly, several newly recognized tick-borne pathogens have emerged joining those already known to be transmitted by ticks. together these factors have led to an enhanced public health awareness of ticks, tick-borne agents, and their associated diseases. reports that several of these agents are transmitted by blood transfusion have raised concerns about blood safety. the primary age ...	2004	15497129
sequence and expression analysis of the ompa gene of rickettsia peacockii, an endosymbiont of the rocky mountain wood tick, dermacentor andersoni.	the transmission dynamics of rocky mountain spotted fever in montana appears to be regulated by rickettsia peacockii, a tick symbiotic rickettsia that interferes with transmission of virulent rickettsia rickettsii. to elucidate the molecular relationships between the two rickettsiae and glean information on how to possibly exploit this interference phenomenon, we studied a major rickettsial outer membrane protein gene, ompa, presumed to be involved in infection and pathogenesis of spotted fever ...	2004	15528527
provision of a lingual biting platform in a severe class iii malocclusion: a case report.		2004	321730
the treatment of acute cholecystitis. a series of 497 consecutive patients.	in a five-year series of 497 consecutive patients with acute cholecystitis an overall mortality of 3.2% was observed. there was no significant difference in mortality between patients undergoing early surgery and those managed initially conservatively. the total incidence of postoperative complications was also similar in the early and delayed surgery groups, although wound complications were slightly more frequent after early surgery. a special risk group was formed by patients with unsuccessfu ...	2004	525185
colonic adrenocarcinomas in patients with ureterosigmoidostomies.	two additional cases of adenocarcinoma of the sigmoid colon occurring at the site of ureterosigmoidostomy are reported. the high risk of this complication occurring in patients who have undergone ureterosigmoidostomy is described. the possible shortness of the interval from diversion to development of an adenocarcinoma, especially in those cases involving carcinoma of the bladder, is emphasized. it is strongly recommended that careful follow up of such patients should begin within several years ...	2004	446246
[the late david kohn, m.d].		2004	321306
[contribution of the section medical pedagogy to the improvement of quality and effectiveness of health care].		2004	425577
[our experience in using artificial bands for ligamentoplasty in the knee region (author's transl)].		2004	509254
five-year findings of the hypertension detection and follow-up program. ii. mortality by race-sex and age. hypertension detection and follow-up program cooperative group.	data are reported for four race-sex and three age subgroups of the hypertension detection and follow-up program (hdfp). throughout the hdfp trial, for black men, black women, white men, and white women and for persons aged 30 to 49, 50 to 59, and 60 to 69 years at entry, control of blood pressure was consistently better for stepped care (sc) than referred care (rc) participants. this difference in degree of control was least for white women; it was less for whites than for blacks of the same sex ...	2004	490883
coronary artery aneurysm.	the case is presented of a 28-year-old woman with systemic vasculitis, aortic insufficiency, and an aneurysm of the proximal right coronary artery. more than 100 patients with coronary artery aneurysm have been reported, and this represents the twenty-first case treated surgically. coronary artery aneurysms occur more frequently than previously realized and are seen in approximately 1.5% of patients with coronary artery disease. in addition to arteriosclerotic aneurysm, other common types of cor ...	2004	305770
[theodor billroth (1829-1894), "pope of surgery", on the 150th anniversary of his birth].		2004	375156
theory and development of transcutaneous oxygen pressure measurement.		2004	3323065
expression of inducible angiosperm promoters in a gymnosperm, picea glauca (white spruce).	electrical discharge particle acceleration was used to test the transient expression of numerous inducible angiosperm promoters in a gymnosperm picea glauca (white spruce). promoter expression was assayed in three different tissues capable of in vitro regeneration, zygotic embryos, seedlings and embryogenic callus. the promoters tested include the light-inducible arabidopsis and soybean ribulose-1,5-bisphosphate small subunit promoters and a maize phosphoenolpyruvate carboxylase promoter; a soyb ...	2003	1868222
identification of actin kinase activity in purified fragmin-actin complex.	actin kinase phosphorylates actin of fragmin-actin complex, resulting in the inactivation of the nucleation and capping activities of the complex. fragmin-actin complex was prepared by a new purification procedure. incubation with atp caused inactivation of the purified complex and phosphorylation of actin of fragmin-actin complex. the detailed analysis of the complex by sds-gel electrophoresis showed that actin kinase was co-purified with the fragmin-actin complex. formation of such an associat ...	2003	1326442
guidelines on after care for denture patients. council on dental health.		2003	193879
[transketolase activity as a parameter for the vitamin b1 provision in some dermatoses (herpes zoster, psoriasis, drug eruptions, excema, and urticaria) (author's transl)].		2003	153861
effects of potassium azide on soil microbial populations and soil enzymatic activities.	preplant applications of potassium azide (kn3) to pine nursery beds were evaluated for effect on the soil microflora and on soil enzyme activity where either plastic-sealing or water-sealing techniques were used. two weeks after incorporation of azide (0-224 kg/hs), soil samplings revealed reduced populations of bacteria and fungi and a corresponding decline in invertase and amylase activities. these effects were proportionate to the amount of azide used and were more pronounced in plastic-seale ...	2003	164268
[prevention of recurring adhesions after tuboplasty. ii. importance of grafts with free pezitoneum (author's transl)].	we have greatly improved in prevention of adhesions after tuboplasty, using peritoneal grafts on crude surfaces (28 cases off 92 tuboplasties). first results seem to confirm the interest of this method.	2003	161984
serologically detected "new" tick-borne zoonoses in eastern croatia.	to establish serologically a contact with causative agents of human monocytic and granulocytic ehrlichiosis, human babesiosis, recently detected rickettsioses, and lyme disease in individuals with a history of tick bite from three counties in eastern croatia.	2003	14515426
[study on spotted fever group rickettsiae in guangdong province].	to explore the existence of spotted fever group rickettsiae (sfgr) in guangdong province.	2003	14521792
mycobacterium marinum escapes from phagosomes and is propelled by actin-based motility.	mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. numerous intracellular pathogens, including listeria monocytogenes, shigella flexneri, and rickettsia rickettsii, exploit the host cytoskeleton by using actin-based motility for cell to cell spread during infection. here we show that mycobacterium marinum, a natural pathogen of fish and frogs and an occa ...	2003	14597736
isolation of bartonella washoensis from a dog with mitral valve endocarditis.	we report the first documented case of bartonella washoensis bacteremia in a dog with mitral valve endocarditis. b. washoensis was isolated in 1995 from a human patient with cardiac disease. the main reservoir species appears to be ground squirrels (spermophilus beecheyi) in the western united states. based on echocardiographic findings, a diagnosis of infective vegetative valvular mitral endocarditis was made in a spayed 12-year-old female doberman pinscher. a year prior to presentation, the re ...	2003	14605197
implications of presumptive fatal rocky mountain spotted fever in two dogs and their owner.	a dog was examined because of petechiation, an inability to stand, pale mucous membranes, a possible seizure, and thrombocytopenia. tick-borne illness was suspected, but despite treatment, the dog died. eight days later, a second dog owned by the same individual also died. the dog was not examined by a veterinarian, but rocky mountain spotted fever (rmsf) was suspected on the basis of clinical signs. two weeks after the second dog died, the owner was examined because of severe headache, fever, n ...	2003	14627095