Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| incidence of congenital defects in south wales, 1964-6. | 2015 | 4996395 | |
| interest charge on unpaid bills. | 2015 | 4406679 | |
| letter: medical writing. | 2015 | 4406071 | |
| [regulations for nurses' legal responsibility]. | 2015 | 4484010 | |
| [insulin receptors and mechanism of the action of insulin in the body]. | 2015 | 4374697 | |
| [hypocalcemic pseudo-deficient vitamin resistant rickets]. | 2015 | 4350439 | |
| massive ascites due to pancreatic stones. | 2015 | 4502769 | |
| radiation hepatitis in children. | 2015 | 4321600 | |
| [long term results of transtemporal neurectomy of the vestibular nerve in menière's disease (author's transl)]. | 2015 | 4420512 | |
| spin concentration per living cell of normal liver, regenerating liver, and hepatomas. | 2015 | 4363447 | |
| a group therapy experience with regressed patients. | 2015 | 4311705 | |
| [the activity of factor xiii in plasma of children with acute leucaemia (author's transl)]. | 2015 | 4518176 | |
| [total ligation of the esophagus by murphy's button. hemmorrhages caused by esophageal varices in portal hypertension. apropos of 34 cases]. | 2015 | 4549250 | |
| observations on pulmonary function testing and blood-gas analysis in children. | 2015 | 4503927 | |
| [q beta rna replicase]. | 2015 | 4567707 | |
| [caucasian period of the activity of v. s. bobrovskiĭ (on the 100th anniversary of his birth)]. | 2015 | 4592872 | |
| comprehensive analysis of a vibrio parahaemolyticus strain extracellular serine protease vpsp37. | proteases play an important role in the field of tissue dissociation combined with regenerative medicine. during the years new sources of proteolytic enzymes have been studied including proteases from different marine organisms both eukaryotic and prokaryotic. herein we have purified a secreted component of an isolate of vibrio parahaemolyticus, with electrophoretic mobilities corresponding to 36 kda, belonging to the serine proteases family. sequencing of the n-terminus enabled the in silico id ... | 2015 | 26162075 |
| a novel adhesive factor contributing to the virulence of vibrio parahaemolyticus. | bacterial adhesins play a pivotal role in the tight bacteria-host cells attachment to initiate the downstream processes and bacterial infection of hosts. in this study, we identified a novel adhesin, vpadf in v. parahaemolyticus. deletion of vpadf in v. parahaemolyticus markedly impaired its attachment and cytotoxicity to epithelial cells, as well as attenuated the virulence in murine model. biochemical studies revealed that vpadf recognized both fibronectin and fibrinogen. the binding of vpadf ... | 2015 | 26399174 |
| prevalence and genetic diversity of clinical vibrio parahaemolyticus isolates from china, revealed by multilocus sequence typing scheme. | the population structure of clinical vibrio parahaemolyticus isolates spreading in china remains undefined. we brought 218 clinical isolates from the pubmlst database originating from different regions of china collected since the year of 1990, analyzed by multilocus sequence typing (mlst), to elucidate the prevalence and genetic diversity of v. parahaemolyticus circulating in chinese population. the mlst scheme produced 137 sequence types (sts). these sts were clustered into six clonal complexe ... | 2015 | 25914691 |
| [pollution of our water and its effect on drinking water condition]. | 2015 | 4692508 | |
| therapeutic aspects of diagnostic radiology in childhood. 2. abnormal meconium and 'milk inspissation'. | 2015 | 4678184 | |
| lead and cadmium content of some vegetable foodstuffs. | 2015 | 4676679 | |
| renal cortical index and calyx index for assessment of development and regression of stasis in congenital hydronephrosis. | 2015 | 4669394 | |
| n.m.r. studies of chlorinated polycyclodiene pesticides. | 2015 | 4667449 | |
| [professor wallace orgood fenn (1893-1971)]. | 2015 | 4619488 | |
| [preliminary results in the comparative quantitative histology of the olfactory bulb from the insectivores to the primates]. | 2015 | 4569271 | |
| [continuous flow system for the determination of urinary 17-hydroxycorticosteroids (porter-silber chromogens) by autoanalyzer]. | 2015 | 4675456 | |
| [large saphenous vein as an arterial replacement]. | 2015 | 4540450 | |
| [mikulicz' disease]. | 2015 | 4523031 | |
| a comparative study of the coats of chimaeric mice and those of heterozygotes for x-linked genes. | 2015 | 4561716 | |
| [diagnosis of adult diseases in agricultural districts]. | 2015 | 4490853 | |
| [the young nurse and the problem of professional anxiety]. | 2015 | 4485025 | |
| preinfarction angina: current therapeutic considerations. | 2015 | 4542027 | |
| the role of immunofluorescence in the diagnosis of oral vesiculobullous disorders. report of four cases. | 2015 | 4551232 | |
| substituent increments for the 1h-nmr. chemical shifts of the 18- and 19-methyl protons of steroids. part i: 9beta, 10alpha(retro)-steroids. | 2015 | 4465338 | |
| recruitment in radiography. | 2015 | 4462150 | |
| [immunologic basis of the atopic syndrome]. | 2015 | 4440398 | |
| [ethics in dental practice]. | 2015 | 4506460 | |
| oxygen consumption of skeletal muscles relaxing under tension. | 2015 | 4544241 | |
| [myocardial blood supply in patients with coronary heart disease and its influencing by a coronary-effective pharmaco]. | 2015 | 4949707 | |
| the effect on dental caries of topically applied acidulated phosphate-fluoride: results after one year. | 2015 | 4384484 | |
| sewage treatment by means of oxidation ponds. | 2015 | 4432066 | |
| diagnostic abdominal radioisotopic lymphography and radiation therapy. | 2015 | 4333860 | |
| the n.y. dental service corp.--present status. a report. | 2015 | 4519492 | |
| concentration of cyclic 3':5'-adenosine monophosphate and glycogen metabolism in the liver. | 2015 | 4319507 | |
| n-terminal aspartate, glycine and serine in poliovirus capsid protein. | 2015 | 4357935 | |
| [connective tissue tumors of the soft parts (apropos of 30 cases)]. | 2015 | 4334588 | |
| the opportunistic marine pathogen vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. | acute hepatopancreatic necrosis disease (ahpnd) is a severe, newly emergent penaeid shrimp disease caused by vibrio parahaemolyticus that has already led to tremendous losses in the cultured shrimp industry. until now, its disease-causing mechanism has remained unclear. here we show that an ahpnd-causing strain of v. parahaemolyticus contains a 70-kbp plasmid (pva1) with a postsegregational killing system, and that the ability to cause disease is abolished by the natural absence or experimental ... | 2015 | 26261348 |
| complete nucleotide sequence of a conjugative plasmid carrying bla(per-1). | the nucleotide sequence of a self-transmissible plasmid pvph1 harboring bla(per-1) from vibrio parahaemolyticus was determined. pvph1 was 183,730 bp in size and shared a backbone similar to paqu1 and paqu2, differing mainly in an ∼40-kb multidrug resistance (mdr) region. a complex class 1 integron was identified together with iscr1 and bla(per-1) (iscr1-bla(per-1)-gst-abct-qaceδ1-sul1), which was shown to form a circular intermediate playing an important role in the dissemination of bla(per-1). | 2015 | 25779581 |
| photorhabdus insect-related (pir) toxin-like genes in a plasmid of vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (ahpnd) of shrimp. | the 69 kb plasmid pvpa3-1 was identified in vibrio parahaemolyticus strain 13‑028/a3 that can cause acute hepatopancreatic necrosis disease (ahpnd). this disease is responsible for mass mortalities in farmed penaeid shrimp and is referred to as early mortality syndrome (ems). the plasmid has a gc content of 45.9% with a copy number of 37 per bacterial cell as determined by comparative quantitative pcr analyses. it consists of 92 open reading frames that encode mobilization proteins, replication ... | 2015 | 25667334 |
| comparative secretomics reveals novel virulence-associated factors of vibrio parahaemolyticus. | vibrio parahaemolyticus is a causative agent of serious human seafood-borne gastroenteritis disease and even death. in this study, for the first time, we obtained the secretomic profiles of seven v. parahaemolyticus strains of clinical and food origins. the strains exhibited various toxic genotypes and phenotypes of antimicrobial susceptibility and heavy metal resistance, five of which were isolated from aquatic products in shanghai, china. fourteen common extracellular proteins were identified ... | 2015 | 26236293 |
| dual function of a bacterial protein as an adhesin and extracellular effector of host gtpase signaling. | bacterial pathogens often target conserved cellular mechanisms within their hosts to rewire signaling pathways and facilitate infection. rho gtpases are important nodes within eukaryotic signaling networks and thus constitute a common target of pathogen-mediated manipulation. a diverse array of microbial mechanisms exists to interfere with rho gtpase signaling. while targeting of gtpases by secreted bacterial effectors is a well-known strategy bacterial pathogens employ to interfere with the hos ... | 2015 | 26156628 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |
| homologs of the acinetobacter baumannii acei transporter represent a new family of bacterial multidrug efflux systems. | multidrug efflux systems are a major cause of resistance to antimicrobials in bacteria, including those pathogenic to humans, animals, and plants. these proteins are ubiquitous in these pathogens, and five families of bacterial multidrug efflux systems have been identified to date. by using transcriptomic and biochemical analyses, we recently identified the novel acei (acinetobacter chlorhexidine efflux) protein from acinetobacter baumannii that conferred resistance to the biocide chlorhexidine, ... | 2015 | 25670776 |
| novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing. | two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [qs]). the basic design of these escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of qs signal molecules. consequently, these e. coli strains are unable to grow in the presence of the respective qs signal molecules unless a nontoxic qs-interfering compound is present. the first reporter strain designed to ... | 2015 | 25527543 |
| strategies of vibrio parahaemolyticus to acquire nutritional iron during host colonization. | iron is an essential element for the growth and development of virtually all living organisms. as iron acquisition is critical for the pathogenesis, a host defense strategy during infection is to sequester iron to restrict the growth of invading pathogens. to counteract this strategy, bacteria such as vibrio parahaemolyticus have adapted to such an environment by developing mechanisms to obtain iron from human hosts. this review focuses on the multiple strategies employed by v. parahaemolyticus ... | 2015 | 26217331 |
| identification of a novel clip domain serine proteinase (sp-csp) and its roles in innate immune system of mud crab scylla paramamosain. | clip domain serine proteinases and their homologs are involved in the innate immunity of invertebrates. to identify the frontline defense molecules against pathogenic infection, we isolated a novel clip domain serine proteinase (sp-csp) from the hemocytes of mud crab scylla paramamosain. the full-length 1362 bp sp-csp contains a 1155 bp open reading frame (orf) encoding 384 amino acids. multiple alignment analysis showed that the putative amino acid sequence of sp-csp has about 52% and 51% ident ... | 2015 | 26272638 |
| identification, characterization, and functional studies of a pelle gene in the chinese mitten crab, eriocheir sinensis. | the toll-like receptor/nf-κb signaling pathways play an important role in the innate immune system. in the present study, one pelle gene (named espelle) was identified for the first time from the chinese mitten crab eriocheir sinensis. the full-length cdna of espelle is 3797 bp with a 3156 bp-long open reading frame that encodes a 1051 amino acid protein. espelle protein contains a death domain at the n-terminal and a serine/threonine kinase domain at the c-terminal. a neighbor joining phylogene ... | 2015 | 26026692 |
| molecular characterization of a transmembrane c-type lectin receptor gene from ayu (plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages. | c-type lectin receptors (ctlrs) play vital roles in immune responses as pattern-recognition receptors (prrs). in this study, we identified a novel c-type lectin receptor (pactlrc) gene from ayu, plecoglossus altivelis. predicted pactlrc is a single transmembrane receptor with a typical carbohydrate recognition domain (crd) at its c-terminus. sequence comparison and phylogenetic tree analysis showed that pactlrc was most closely related to atlantic salmon (salmo salar) clrc, but was significantly ... | 2015 | 26010409 |
| a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis). | integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ... | 2015 | 26004499 |
| identification and molecular characterization of a peritrophin-like gene, involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | peritrophin was first isolated from insect peritrophic membrane (pm) and was thought to protect insects from invasion of microorganisms and to stimulate digestion of food. in this study, a peritrophin-like gene (espt) was obtained from eriocheir sinensis. the full length cdna of espt was 1232 bp, which contained 1005 bp orf encoding a protein of 334 amino acids, including a 22 amino acid signal peptide, and 3 conserved chitin binding type 2 domains (chtbd2) characterized by having a 6-cysteine m ... | 2015 | 25596425 |
| biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. | in the present study, the strain brevibacterium frigoritolerans dc2 was explored for the efficient and extracellular synthesis of silver nanoparticles. these biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. in addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. the dynamic l ... | 2015 | 25848272 |
| enteric bacteria isolated from diarrheal patients in korea in 2014. | the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease. | 2015 | 26473090 |
| starch based biodegradable graft copolymer for the preparation of silver nanoparticles. | the synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (pma) grafted amylopectin (ap) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. the prepared graft copolymer was biodegradable which was shown by fungal growth. characterization of silver nanoparticles was carried out by uv-vis spectroscopy (417nm), hr-tem, saed and fesem analysis. the tem fi ... | 2015 | 26231330 |
| identification and functional analysis of a hemolin like protein from litopenaeus vannamei. | hemolin is a specific immune protein belonging to immunoglobulin superfamily and firstly identified in insects. growing evidences suggest that hemolin can be activated by bacterial and viral infections and may play an important role in antimicrobial immunity. in this paper, we firstly identified a hemolin-like protein from litopenaeus vannamei (lvhemolin). sequence analysis showed that lvhemolin shares high similarity with insect hemolins and is mainly composed of seven immunoglobulin (ig) domai ... | 2015 | 25527138 |
| enzymatic elucidation of haemocyanin from kuruma shrimp marsupenaeus japonicus and its molecular recognition mechanism towards pathogens. | haemocyanin is an important non-specific immune protein present in the hemolymph of invertebrates, which have the ability to recognize the microbial pathogens and trigger the innate immune system. in this study, we isolated and purified the haemocyanin using gel filtration chromatography and investigated its microbial recognition mechanism against the invading pathogens. kuruma shrimp marsupenaeus japonicus haemocyanin showed the single band with a molecular weight of 76 kda on sds-page and its ... | 2015 | 25204648 |
| evaluation of ice slurries as a control for postharvest growth of vibrio spp. in oysters and potential for filth contamination. | raw oyster consumption is the most common route of exposure for vibrio spp. infections in humans. vibriosis has been increasing steadily in the united states despite efforts to reduce the incidence of the disease. research has demonstrated that ice is effective in reducing postharvest vibrio spp. growth in oysters but has raised concerns of possible contamination of oyster meat by filth (as indicated by the presence of fecal coliform bacteria or clostridium perfringens). this study examined the ... | 2015 | 26197291 |
| isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture. | acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ... | 2015 | 25737203 |
| a probe-free four-tube real-time pcr assay for simultaneous detection of twelve enteric viruses and bacteria. | we aim to develop a multiplex real-time pcr assay to detect the most common pathogens causing community outbreaks of diarrhea. | 2015 | 26342434 |
| molecular insights into a molluscan transferrin homolog identified from disk abalone (haliotis discus discus) evidencing its detectable role in host antibacterial defense. | the basic function of transferrin is to bind iron (iii) ions in the medium and to deliver them to the locations where they are required for metabolic processes. it also takes part in the host immune defense mainly via its ability to bind to iron (iii) ions. hence, transferrin is also identified as an important acute-phase protein in host immunity. abalones are major shellfish aquaculture crops that are susceptible to a range of marine microbial infections. since transferrin is known to be a majo ... | 2015 | 26191782 |
| cloning and characterization of a novel hemocyanin variant lvhmcv4 from shrimp litopenaeus vannamei. | recently, we found 3 variants of hemocyanin subunit with higher molecular weight in shrimp litopenaeus vannamei (named as lvhmcv1-3). in this study, a novel l. vannamei hemocyanin variant (named as lvhmcv4) was further cloned and characterized. bioinformatic analysis predicted that lvhmcv4 contains one open reading frame of 2137 bp and encodes a polypeptide of 678 amino acids. it shares 84-99% cdna sequences identity to that of the classical form of l. vannamei hemocyanin (lvhmc, aj250830.1) and ... | 2015 | 26115633 |
| identification and purification of the cpd photolyase in vibrio parahaemolyticus rimd2210633. | photoreactivation is an error-free mechanism of dna repair, utilized by prokaryotes and most eukaryotes and is catalyzed by specific enzymes called dna photolyases. photoreactivation has been reported in vibrio parahaemolyticus wp28; however, information on photolyases in v. parahaemolyticus (v.p) strains has not been reported. this study examined the photoreactivation in v.p rimd2210633. the photolyase responsible for repairing cyclobutane pyrimidine dimer (cpd) in dna was identified, and the c ... | 2015 | 26104028 |
| protein transduction domain of transactivating transcriptional activator fused to outer membrane protein k of vibrio parahaemolyticus to vaccinate marbled eels (anguilla marmorata) confers protection against mortality caused by v. parahaemolyticus. | although immersion and oral vaccination are the most practical methods for fish farmers, their applications are very limited due to low immune stimulation effect. we used the protein transduction domain (ptd) of transactivating transcriptional factor (tat) derived from hiv tat protein to increase the delivery efficiency of aquatic protein vaccines. vibrio parahaemolyticus outer membrane protein k (ompk), a reported vaccine candidate for the prevention of v. parahaemolyticus infection, was fused ... | 2015 | 25919337 |
| hygiene and health risks associated with the consumption of edible lamellibranch molluscs. | edible lamellibranch molluscs are frequently the cause of very serious outbreaks of food poisoning. microbiological suitability for human consumption, according to ec reg. n. 1441/2007, is only based on two faecal bacteriological contamination parameters (escherichia coli and salmonella) and does not consider other microorganisms which are naturally present within the marine environment and are potentially pathogenic such as those belonging to the genus vibrio. in this study, a total of 540 samp ... | 2015 | 25744696 |
| saccharification of β-chitin from squid pen by a fermentation method using recombinant chitinase-secreting escherichia coli. | two strains [bl21(de3) and hms174(de3)] of escherichia coli harboring the recombinant chitinase expression plasmid pvp-chi, which contains vibrio parahaemolyticus chitinase gene with an attached signal sequence, were prepared. these e. coli transformants produced a large amount of recombinant chitinase, which hydrolyzes chitin to yield di-n-acetylchitobiose (glcnac)2, under the presence of isopropyl-1-thio-β-d-galactopyranoside (iptg), and secreted the enzyme into their culture fluid with the ai ... | 2015 | 25725799 |
| the expression of propionicin plg-1 gene (plg-1) by lactic starters. | propionicin plg-1 is a bacteriocin produced by propionibacterium thoenii p127. such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic escherichia coli, pseudomonas aeruginosa, vibrio parahaemolyticus, yersinia enterocolitica and a strain of corynebacterium sp. in the present study, plg-1 gene expressing propionicin plg-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. the pcr product cont ... | 2015 | 25684078 |
| molecular cloning and functional characterization of a qm protein in large yellow croaker (larimichthys crocea). | since it was proposed to be a tumor suppressor in 1991, qm protein has attracted intensive and wide attention in plants, animals and fungi research fields. up to date, however, the function of qm protein in fish immunity remains unknown. in this investigation, a qm gene (named as lycqm gene) was cloned from large yellow croaker (larimichthys crocea), and lycqm protein was expressed in escherichia coli and purified. the lycqm gene was ubiquitously transcribed in multi-tissues, including spleen, m ... | 2015 | 25680268 |
| exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. | multidrug-resistant bacteria are an increasingly serious threat to human and animal health. however, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant edwardsiella tarda by gc-ms-based metabolomics. exogenous alanine or glucose restores susceptibility of multidrug-resistant e. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resista ... | 2015 | 25651179 |
| daily variations in pathogenic bacterial populations in a monsoon influenced tropical environment. | changing climatic conditions have influenced the monsoon pattern in recent years. variations in bacterial population in one such tropical environment were observed everyday over two years and point out intra and inter annual changes driven by the intensity of rainfall. vibrio spp. were abundant during the monsoon and so were faecal coliforms. vibrio alginolyticus were negatively influenced by nitrate, whereas, silicate and rainfall positively influenced vibrio parahaemolyticus numbers. it is als ... | 2015 | 25956443 |
| antimicrobial properties of a potential probiotic lactobacillus from thai newborn feces. | probiotics are increasingly used to treat infectious diarrhea and antibiotic-associated diarrhea. many probiotic bacteria are classified in general such as lactobacillus and are able to colonize the gastrointestinal tracts of infants. | 2015 | 26817219 |
| deciphering the role of multiple betaine-carnitine-choline transporters in the halophile vibrio parahaemolyticus. | vibrio parahaemolyticus is a halophile that is the predominant cause of bacterial seafood-related gastroenteritis worldwide. to survive in the marine environment, v. parahaemolyticus must have adaptive strategies to cope with salinity changes. six putative compatible solute (cs) transport systems were previously predicted from the genome sequence of v. parahaemolyticus rimd2210633. in this study, we determined the role of the four putative betaine-carnitine-choline transporter (bcct) homologues ... | 2015 | 25344241 |
| quantifying viable vibrio parahaemolyticus and listeria monocytogenes simultaneously in raw shrimp. | a novel taqman-based multiplex real-time pcr method combined with propidium monoazide (pma) treatment was firstly developed for the simultaneous quantification of viable vibrio parahaemolyticus and listeria monocytogenes in raw shrimp. the optimization of pma concentration showed that 100 μm was considered optimal to effectively inhibit 10(8) cfu/ml dead cells of both bacteria. the high specificity of this method was confirmed on tests using 96 target and non-target strains. the optimized assay ... | 2015 | 26048473 |
| two variants of selenium-dependent glutathione peroxidase from the disk abalone haliotis discus discus: molecular characterization and immune responses to bacterial and viral stresses. | glutathione peroxidase (gpx) is an essential member of the antioxidant systems of living organisms and may be involved in immune defense against pathogenic invasion. in the current study, two selenium-dependent glutathione peroxidases (absegpxs) that shared 54.3% identity were identified from the disk abalone haliotis discus discus. the open reading frames (orfs) of absegpx-a and absegpx-b coded for 222 and 220 amino acids, respectively, with a characteristic selenocysteine residue encoded by an ... | 2015 | 26025184 |
| three novel clade b serine protease inhibitors from disk abalone, haliotis discus discus: molecular perspectives and responses to immune challenges and tissue injury. | serine protease inhibitors (serpins) control cellular protease activity in order to maintain cellular homeostasis. the immune and inflammatory responses of invertebrate clade b serpins have not been widely reported. in the present study, three proteins with high similarity to clade b serpins, referred to as abserpin-1, abserpin-2 and abserpin-3, were identified from disk abalone (haliotis discus discus). while abserpin-1 (399 aa) was of a typical size for this protein class, abserpin-2 (506 aa) ... | 2015 | 25917971 |
| effect of sodium-alginate and laminaran on salmonella typhimurium infection in human enterocyte-like ht-29-luc cells and balb/c mice. | brown algal polysaccharides such as alginate, polymers of uronic acids, and laminaran, beta-1,3 and 1,6-glucan, can be fermented by human intestinal microbiota. to evaluate the effects of these polysaccharides on infections caused by food poisoning pathogens, we investigated the adhesion and invasion of pathogens (salmonella typhimurium, listeria monocytogenes and vibrio parahaemolyticus) in human enterocyte-like ht-29-luc cells and in infections caused in balb/c mice. both sodium na-alginate an ... | 2015 | 25857966 |
| simultaneous construction of pcr-dgge-based predictive models of listeria monocytogenes and vibrio parahaemolyticus on cooked shrimps. | the aim of this study was to simultaneously construct pcr-dgge-based predictive models of listeria monocytogenes and vibrio parahaemolyticus on cooked shrimps at 4 and 10°c. calibration curves were established to correlate peak density of dgge bands with microbial counts. microbial counts derived from pcr-dgge and plate methods were fitted by baranyi model to obtain molecular and traditional predictive models. for l. monocytogenes, growing at 4 and 10°c, molecular predictive models were construc ... | 2015 | 25470339 |
| subversion of the cytoskeleton by intracellular bacteria: lessons from listeria, salmonella and vibrio. | entry into host cells and intracellular persistence by invasive bacteria are tightly coupled to the ability of the bacterium to disrupt the eukaryotic cytoskeletal machinery. herein we review the main strategies used by three intracellular pathogens to harness key modulators of the cytoskeleton. two of these bacteria, namely listeria monocytogenes and salmonella enterica serovar typhimurium, exhibit quite distinct intracellular lifestyles and therefore provide a comprehensive panel for the under ... | 2015 | 25440316 |
| phylogenetic diversity and antibacterial activity of culturable fungi derived from the zoanthid palythoa haddoni in the south china sea. | investigation on diversity of culturable fungi mainly focused on sponges and corals, yet little attention had been paid to the fungal communities associated with zoanthid corals. in this study, a total of 193 culturable fungal strains were isolated from the zoanthid palythoa haddoni collected in the south china sea, of which 49 independent isolates were identified using both morphological characteristics and internal transcribed spacer (its) sequence analyses. thirty-five strains were selected f ... | 2015 | 25117478 |
| characterization and pcr detection of binary, pir-like toxins from vibrio parahaemolyticus isolates that cause acute hepatopancreatic necrosis disease (ahpnd) in shrimp. | unique isolates of vibrio parahaemolyticus (vpahpnd) have previously been identified as the causative agent of acute hepatopancreatic necrosis disease (ahpnd) in shrimp. ahpnd is characterized by massive sloughing of tubule epithelial cells of the hepatopancreas (hp), proposed to be induced by soluble toxins released from vpahpnd that colonize the shrimp stomach. since these toxins (produced in broth culture) have been reported to cause ahpnd pathology in reverse gavage bioassays with shrimp, we ... | 2015 | 26017673 |
| prevalence, biochemical characteristics, and antibiotic susceptibility of aeromonads, vibrios, and plesiomonads isolated from different sources at a zoo. | aeromonas spp., vibrio parahaemolyticus , and plesiomonas shigelloides are commonly implicated in foodborne and waterborne diarrheal illnesses of humans and other animals. the present study assessed the prevalence, biochemical characteristics, and antibiotic susceptibility of aeromonas spp., v. parahaemolyticus , and p. shigelloides by analyzing samples from 729 sources at a zoo, including animal feces (n=607), watering facilities (n=104), and pond water samples (n=18). of the 729 samples collec ... | 2015 | 26056883 |
| vibrio effector protein vopq inhibits fusion of v-atpase-containing membranes. | vesicle fusion governs many important biological processes, and imbalances in the regulation of membrane fusion can lead to a variety of diseases such as diabetes and neurological disorders. here we show that the vibrio parahaemolyticus effector protein vopq is a potent inhibitor of membrane fusion based on an in vitro yeast vacuole fusion model. previously, we demonstrated that vopq binds to the v(o) domain of the conserved v-type h(+)-atpase (v-atpase) found on acidic compartments such as the ... | 2015 | 25453092 |
| n-hexanoyl-l-homoserine lactone-degrading pseudomonas aeruginosa psdahp1 protects zebrafish against vibrio parahaemolyticus infection. | four strains of n-hexanoyl-l-homoserine lactone (ahl)-degrading pseudomonas spp., named psdahp1, psdahp2, psdahp3, and psdahp4 were isolated and identified from the intestine of fenneropenaeus indicus. psdahp1 showed the highest ahl-degrading activity among the four isolates. psdahp1 inhibited biofilm-forming exopolysaccharide and altered cell surface hydrophobicity of virulent green fluorescent protein (gfp)-tagged vibrio parahaemolyticus dahv2 (gfp-vpdahv2). oral administration of psdahp1 sign ... | 2015 | 25449384 |
| function of a novel c-type lectin with two crd domains from macrobrachium rosenbergii in innate immunity. | c-type lectins play crucial roles in innate immunity. in the present study, a novel c-type lectin gene, designated as mrctl, was identified from macrobrachium rosenbergii. mrctl contains 2 carbohydrate-recognition domains (crds), namely mrcrd1 and mrcrd2. the mrcrd1 contains a qep motif and mrcrd2 contains a motif of epd. mrctl was mainly expressed in the hepatopancreas. the expression level of mrctl in hepatopancreas was significantly upregulated after a challenge with vibrio parahaemolyticus o ... | 2015 | 25475962 |
| vibrio alginolyticus associated chronic myringitis acquired in mediterranean waters of turkey. | vibrio alginolyticus was originally classified as biotype 2 of vibrio parahaemolyticus. most clinical isolates are recovered from superficial wounds or the external ear infections. v. alginolyticus is acknowledged to be nearly nonpathogenic in humans. the reason for presence of v. alginolyticus's virulence is uncertain. we describe a chronic myringitis case in a 47-year-old female due to v. alginolyticus. according to her anamnesis, it was detected that she had sea bathing history in mugla coast ... | 2015 | 26605095 |
| flightless-i (flii) is a potential negative regulator of the toll pathway in litopenaeus vannamei. | flightless-i (flii) is a protein negatively modulates the toll-like receptor (tlr) pathway through interacting with myeloid differentiation factor 88 (myd88). to investigate the function of flii in innate immune responses in invertebrates, litopenaeus vannamei flii (lvflii) was identified and characterized. the full-length cdna of lvflii is 4, 304 bp long, with an open reading frame (orf) encoding a putative protein of 1292 amino acids, including 12 leucine-rich repeat (lrr) domains at the n-ter ... | 2015 | 25449702 |
| prevalence and antimicrobial resistance of vibrio spp. in retail and farm shrimps in ecuador. | the aim of this study was to investigate the prevalence of vibrio spp. in shrimp at retail and in shrimp farms in ecuador and to determine the antimicrobial agent resistance patterns of farm isolates. the presence of genes linked to early mortality syndrome (ems) or acute hepatopancreatic necrosis disease (ahpnd) also was evaluated. vibrio spp. were isolated from retail shrimps in cuenca, ecuador, and farm shrimps originating from provinces el oro and guayas, ecuador. a total of 229 shrimp sampl ... | 2015 | 26555534 |
| a novel innexin2 forming membrane hemichannel exhibits immune responses and cell apoptosis in scylla paramamosain. | innexins are a class of transmembrane proteins that are important for embryonic development, morphogenesis and electrical synapse formation. in the present study, a novel innexin2 gene from scylla paramamosain was named sp-inx2 and characterized. the complete cdna and genomic dna sequences of sp-inx2 were revealed. sp-inx2 mrna transcripts were distributed in various tissues of s. paramamosain and were most abundant in the hemocytes. the sp-inx2 was significantly upregulated in hemocyte, gill an ... | 2015 | 26384843 |
| prevalence of vibrio spp. in raw shrimps (parapenaeus longirostris) and performance of a chromogenic medium for the isolation of vibrio strains. | vibrios are natural inhabitants of estuarine ecosystems and some species may pose public health problem as agents of sporadic or collective food-borne infections associated with the consumption of fish or shellfish. samples of raw shrimp (n = 299), fished in coastal areas of the city of agadir, morocco, and collected from its fish marketplace, were examined for the presence of pathogenic vibrios. microbiological analysis was carried out according to a protocol using thiosulphate citrate bile suc ... | 2015 | 26081523 |
| deep-sea hydrothermal vent bacteria related to human pathogenic vibrio species. | vibrio species are both ubiquitous and abundant in marine coastal waters, estuaries, ocean sediment, and aquaculture settings worldwide. we report here the isolation, characterization, and genome sequence of a novel vibrio species, vibrio antiquarius, isolated from a mesophilic bacterial community associated with hydrothermal vents located along the east pacific rise, near the southwest coast of mexico. genomic and phenotypic analysis revealed v. antiquarius is closely related to pathogenic vibr ... | 2015 | 25964331 |
| rapid proliferation of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae during freshwater flash floods in french mediterranean coastal lagoons. | vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae of the non-o1/non-o139 serotype are present in coastal lagoons of southern france. in these mediterranean regions, the rivers have long low-flow periods followed by short-duration or flash floods during and after heavy intense rainstorms, particularly at the end of the summer and in autumn. these floods bring large volumes of freshwater into the lagoons, reducing their salinity. water temperatures recorded during sampling (15 to 24° ... | 2015 | 26319881 |