Publications

[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].	every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...	1990	2075365
[shigellosis].		1990	2076763
[clinico-morphological comparisons in different types of dysentery].	clinical evidence and morphological picture of rectal biopsy specimens were analysed for 125 dysentery patients. as compared to a light course, moderately severe and severe dysentery presented enhanced microbial adherence and an elevated level of large lymphocytes in the external epithelium of the rectum, deeper penetration of shigella into crypts. on day 9-15 of the disease in attenuation of the clinical symptoms in severe and moderately dysentery morphological shifts persisted whereas the ligh ...	1990	2084341
palindromic unit highly repetitive dna sequences exhibit species specificity within enterobacteriaceae.	palindromic units (pu, or rep for repetitive extragenic palindrome) constitute a family of dna sequences of 40 nucleotides which is highly repeated in the genome of escherichia coli. we analysed the presence of pu sequences in 99 different bacterial genomes by cross-hybridization. when pu sequences were used as a probe, only dna from enterobacteriaceae closely related to e. coli exhibited an appreciable hybridization signal: shigella sonnei, shigella boydii, salmonella enteritica serotype typhim ...	1990	2092362
molecular analysis of strains of shigella boydii isolated in northern and southern italy.	in the years 1981-1988, shigella boydii played a very limited role in the aetiology of diarrhoeal disease in italy. however, between september and november, 1985, 19 isolates of serotype 2 were recovered in northern italy from a dysentery outbreak which occurred in a geriatrics hospital in abbiategrasso (milan, lombardy) and seven were identified in southern italy during the period january-july, 1986 from apparently unrelated infection cases occurring in brindisi (apulia). these isolates were co ...	1990	2092367
effects of chronic non-ketotic diabetes and aging on myocardial function and fatty acid oxidation.	the effects of aging and chronic non-ketotic diabetes on contractile properties, oxygen consumption, palmitate oxidation and morphology were studied in isolated, perfused working hearts of 2, 9, 12 and 22 month old rats. the heart rate, coronary flow, and oxygen consumption were no different among the 9, 12 and 22 month control and diabetic hearts. cardiac work was not depressed in control hearts until 22 months of age. depression of cardiac output due to aging in the control hearts progressed i ...	1990	2141842
[antibiotic resistance of shigella isolated in the kara-kalpak a.s.s.r. 1987-1988].	sensitivity of shigella spp. isolated in one of the hospitals of nukus within 1987-1988 and earlier in 1977 and 1985 was studied. s. flexneri 1-5 remained the main causative agents of dysentery on the territory. however, beginning from 1987 there were registered cases of dysentery caused by s. dysenteriae 1. the isolates were most sensitive to cefotaxime, cephaloridine, polymyxin b and gentamicin. the majority of the isolates were resistant to tetracycline, levomycetin (chloramphenicol) and stre ...	1990	2144726
an attempt to structurally convert mu-selective morphine toward delta-receptor binding: dimerization based on enkephalin conformation.	in order to elucidate the substrate specificities for mu- and delta-opioid receptors, dimerization of the mu-specific morphine molecule was attempted, based on the hypothesis of the possible relationship between the molecular conformation of endogenous enkephalin and its selectivity for each opioid receptor. the nor2 ([normorphine]n-ch2-ch2-n[normorphine]) thus synthesized exhibited agonist activity showing a preference for binding with the delta-opioid receptor, compared with the parent morphin ...	1990	2164938
[the importance of shigella boydii in shigellosis morbidity in the ussr].	the etiological role of s. boydii in shigella infections registered at different territories of the ussr in 1986-1987 was analyzed. as established by this analysis, s. boydii infection occurred mainly at the territories with unsatisfactory water supply of the population; from these territories the infection spread to ther territories of the ussr. the dominating serovars causing s. boydii dysentery, as well as shigella infections, at different territories of the ussr was shown to be identical, wh ...	1990	2195811
polymerase chain reaction for detection of invasive shigella flexneri in food.	the polymerase chain reaction (pcr) was used to amplify a 760-base-pair (bp) fragment with the 220-kbp invasive plasmids of enteroinvasive escherichia coli, shigella flexneri, shigella dysenteriae, shigella boydii, and shigella sonnei as templates. this pcr product was easily detected by agarose gel electrophoresis. a 210-bp acci-psti fragment lying within the amplified region was used as a probe in southern hybridization blots and showed that the pcr-generated product was derived from the invas ...	1990	2200336
plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from nigeria.	in an epidemiological survey, plasmid profiles and antimicrobial susceptibility testing of 100 shigella isolates in lagos, nigeria was done. all the isolates were sensitive to nalidixic acid, nitrofurantoin and ciprofloxacin. the commonest antimicrobial susceptibility pattern was resistance to ampicillin, colistin sulphate, co-trimoxazole, streptomycin and tetracycline. all but 4 of 100 isolates screened contained one or more plasmids. plasmid profile analysis distinguished more strains than did ...	1990	2200702
childhood shigellosis: clinical and bacteriological study.	ninety-three children with shigellosis were hospitalized in a regional hospital in kuwait during the year 1988. s. flexneri was the most common isolate (54%) followed by s. sonnei (39%) and s. boydii (7%). in addition to the gastrointestinal manifestations, 14 patients (15%) developed generalized convulsions. the benign and self-limiting nature of the convulsions associated with shigellosis means that neither diagnostic procedure nor drug therapy are usually necessary. the disease was self-limit ...	1990	2202839
the monitoring of foodhandlers in papua new guinea.	in early 1987 guidelines were re-enforced for pre-employment medicals and 6-monthly health checks on foodhandlers in the ok tedi mining project in papua new guinea. the health monitoring program was stepped up as a result of two typhoid cases imported from the highlands and catering subcontractors failing to comply with the necessary pre-placement medicals for foodhandlers. highlanders made up 28% (49/174) of the ok tedi catering department's workforce in 1987. the initial screen of 155 foodhand ...	1990	2238835
[bacillary dysentery--1988].		1990	2251385
[a new serotype of shigella boydii].	two strains which belong to the same serotype of shigella were isolated from the bloody-pus stool of two patients (in 1986) and is reported in this paper. the results were identical both showing agglutination in low titer with serotype 8 of s. dysenteriae and serotype 4 of s. boydii when the two strains were checked well with all kinds of diagnostic antisera and vice versa, ie the antisera produced by the two strains were also checked well with sera prepared with the representative strains of al ...	1990	2251827
glucocorticoid-regulated gene expression in the immune system. analysis of glucocorticoid-regulated transcripts from the mouse macrophage-like cell line p388d1.	to further elucidate the molecular mechanisms underlying glucocorticoid-mediated immune suppression, we have exploited cdna cloning and subtractive screening methods to identify glucocorticoid-regulated transcripts in the mouse macrophage-like cell line, p388d1. two of the three isolated glucocorticoid-regulated mrna species corresponded to genes potentially important to immunoregulation: one glucocorticoid-suppressed mrna species probably encoded the previously uncloned 3-hydroxy-3-methylglutar ...	1990	2258621
[bacteriology and epidemiology of shigellas isolated at the army hospital in djibouti].	in a space of 26 months 108 shigellas were isolated from 104 patients with sporadic and benign diseases. 90% of the subjects were young french. both s. dysenteriae and s. boydii make up 21% of the strains studied. 13% of the germs are multiresistance. multiresistance can enhance development of serious sickness if a virulent strain comes in.	1990	2263188
[current views on the pathomorphology and pathogenesis of dysentery].	according to the literature and the authors' data in patients who died of dysentery shigellae are found seldom because of postmortem shedding of superficial colonic epithelium infected by them. shigella adhesion and invasion into the colonocytes are regularly found in the colon biopsies. as shown recently in experiments, shigella outer membrane proteins forming "contact haemolysin" ("virulence plasmid" product) are responsible for their invasion. in the small intestine this cytotoxin is destroye ...	1990	2281980
kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate.	the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ...	1990	2365688
monoclonal antibodies to quebec strain (q17) of bovine rotavirus.	fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ...	1990	1698323
transient cytokeratin expression in skeletal muscle during murine embryogenesis.	cytokeratin expression was investigated in paravertebral skeletal musculature of 10 d and 18 d old embryos as well as in adult nmri-mice. the muscular nature of the evaluated tissue was evidenced by their expression of vimentin and desmin. binding moieties for cytokeratin antibodies (polyclonal and monoclonal) could be demonstrated only in muscle cells of 10 d old embryos. concerning subtypes, the mouse equivalents of the human cytokeratins nos. 8, 18 and 19 could be made probable. the importanc ...	1990	1698345
conduction velocity distribution of afferent fibers innervating the rat urinary bladder.	the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ...	1990	1698508
characterization of shigella dysenteriae serotypes 11, 12, and 13.	we conducted serologic and biochemical studies on strains of three provisional shigella serotypes. included were 19 strains of serotype 3873-50 from three countries (1 from mexico, 1 from netherlands antilles, and 17 from the united states), 13 strains of serotype 3341-55 from three countries (1 from canada, 1 from bulgaria, and 11 from the united states), and 19 strains of serotype 19809-73 from two countries (16 from israel and 3 from the united states). reactions of these strains with homolog ...	1990	1701447
control of growth of benzo(a)pyrene-transformed 3t3 cells.	the growth controls observed in benzo[a]pyrene-transformed 3t3 cells (bp3t3) are compared with those of virus-transformed and normal 3t3 cells. superficially, the chemically transformed bp3t3 cells have the same behavior as virus-transformed sv3t3 cells. both grow to high cell density in culture medium with 10% serum, both form colonies in methocel, and both are tumorigenic. closer examination, however, has disclosed that bp3t3 cells exhibit "normal" growth controls at low serum concentrations. ...	1990	184467
treatment of cancer of the pancreas with high energy photons and electrons.		1989	184068
sickle cell crises: psychological factors associated with onset.		1989	267253
immunoglobulin m, a, and g antibody response to lipopolysaccharide o antigen in symptomatic and asymptomatic shigella infections.	the antilipopolysaccharide antibody response in sera obtained from subjects involved in 10 outbreaks of shigellosis occurring in israeli military field units was determined by an enzyme-linked immunosorbent assay and a passive hemagglutination test. both tests were found to be sensitive and specific for the diagnosis of shigellosis. a significant antibody response was detected in 73 to 82% of the symptomatic and 48 to 60% of the asymptomatic subjects during the shigella sonnei and shigella flexn ...	1989	2463995
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
four new provisional serovars of shigella.	four bacterial strains are described that possess the biochemical characteristics of shigella species but do not belong to any of the established shigella serovars or to any previously described provisional serovar. one strain fermented mannitol, and it is proposed that this be the type strain for a new provisional serovar of shigella boydii. the remaining strains did not ferment mannitol and belonged to three different serovars. these strains are proposed as type strains for three new provision ...	1989	2501349
shigellae and vibrionaceae species as a cause of diarrhoea among children in aden.	in aden, the incidence of diarrhoea among infants and children is still high. the present work aimed at investigation of shigelleae and vibrionaceae as etiologic agents of enteric infections. stool specimens obtained from 1056 children complaining of diarrhoea were cultured on plating media and on enrichment media. suspected colonies were then serotyped and antibiotic sensitivity tests performed. seven percent of the specimens were found positive for shigella strains. shigella boydii was the com ...	1989	2519964
high incidence of false positives by a latex agglutination test for the diagnosis of clostridium difficile associated colitis in compromised patients.	detection of clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitis has been used for over a decade. because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (la) kit has attracted much attention. we compared the sensitivity and specificity of this method with the cytotoxic assay (cta) using diarrheal stools from 652 patients at a referral tertiary care center. specimens from 71 (10.9%) pa ...	1989	2591165
causes of death and the histopathologic findings in fatal shigellosis.	thirty-seven children (median age, 2 years) with shigellosis in bangladesh were subjected to postmortem examination to determine causes of death and the spectrum of intestinal histopathology. infecting species were: shigella dysenteriae 1, 7 cases; s. dysenteriae 2, 2 cases; shigella flexneri, 23 cases; shigella boydii, 4 cases; and mixed infection with shigella boydii and shigella sonnei, 1 case. complicating conditions detected before death included malnutrition in 25 cases, pneumonia in 11 ca ...	1989	2594452
bacterial pathogens isolated from cockroaches trapped from paediatric wards in peninsular malaysia.	a survey was conducted in 4 paediatric wards in malaysia to determine the distribution of various species of cockroaches and to examine their gut contents for bacteria. cockroaches were trapped from food dispensing areas (kitchens), store rooms, cupboards and open wards. 104 cockroaches were caught, consisting of periplaneta americana (67.3%), blattella germanica (26%), p. brunnea (4.8%), and supella longipalpa (1.9%). bacteria were isolated from all cockroaches except 3 p. americana. many bacte ...	1989	2603192
cardiopulmonary baroreflex control of brachial artery diameter in sustained essential hypertension.	an exaggerated reflex response of forearm resistance vessels following stimulation of cardiac mechanoreceptors has been observed in human hypertension. in the present study we analyze the possibility that such an increased response also involves the large brachial artery. for that purpose, the influence of leg elevation in subjects in a supine position on brachial artery diameter and blood flow velocity was studied in 12 healthy, normal males and 17 males of the same age with sustained essential ...	1989	2607141
autosomal dominant retinitis pigmentosa (adrp): localization of an adrp gene to the long arm of chromosome 3.	members of a large pedigree of irish origin presenting with early onset type i autosomal dominant retinitis pigmentosa (adrp) have been typed for d3s47 (c17), a polymorphic marker from the long arm of chromosome 3. significant, tight linkage of adrp to d3s47, with a lod score of 14.7 maximizing at 0.00 recombination, has been obtained, hence localizing the adrp gene (rp1) segregating in this pedigree to 3q.	1989	2613244
indomethacin blocks the anorexic action of interleukin-1.	it has been reported recently that the central nervous system actions of interleukin-1 are mediated by the prostaglandin system in the brain. the present study was therefore performed in order to examine the hypothesis that indomethacin, an inhibitor of prostaglandin biosynthesis, might alleviate the interleukin-1-induced suppression of food intake in rats. the i.p. injection of interleukin-1 (2 micrograms/rat) resulted in a significant decrease in food intake. the pre-injection of indomethacin ...	1989	2620696
indomethacin blocks the anorexic action of interleukin-1.	it has been reported recently that the central nervous system actions of interleukin-1 are mediated by the prostaglandin system in the brain. the present study was therefore performed in order to examine the hypothesis that indomethacin, an inhibitor of prostaglandin biosynthesis, might alleviate the interleukin-1-induced suppression of food intake in rats. the i.p. injection of interleukin-1 (2 micrograms/rat) resulted in a significant decrease in food intake. the pre-injection of indomethacin ...	1989	2620696
the effects of acetyl-l-carnitine on experimental models of learning and memory deficits in the old rat.	experimental models of learning and memory deficits in aged rats can be studied by means of behavioural tests that provide an important tool for evaluating the effect of drugs on these parameters. active and passive avoidance tests showed a clear impairment of learning and memory capacity of old rats. these tests were also used to study the behavioural effect of acetyl-l-carnitine in aged rats. the subchronic treatment with this drug was followed by a significant improvement of acquisition and r ...	1989	2620857
the effects of acetyl-l-carnitine on experimental models of learning and memory deficits in the old rat.	experimental models of learning and memory deficits in aged rats can be studied by means of behavioural tests that provide an important tool for evaluating the effect of drugs on these parameters. active and passive avoidance tests showed a clear impairment of learning and memory capacity of old rats. these tests were also used to study the behavioural effect of acetyl-l-carnitine in aged rats. the subchronic treatment with this drug was followed by a significant improvement of acquisition and r ...	1989	2620857
isolated and combined deficiencies of the alpha-keto acid dehydrogenase complexes.		1989	2634350
emergence of highly trimethoprim-sulfamethoxazole-resistant shigella in a native american population: an epidemiologic study.	resistance to trimethoprim-sulfamethoxazole (tmp-smx) emerged among shigella isolates from the navajo reservation in the southwestern united states in 1985, years after this antimicrobial agent came into common use. in the study area, tmp-smx resistance increased dramatically from 3 per cent in 1983 to 21 per cent in 1985. resistance was polyclonal and occurred in both s. sonnei and s. flexneri. no single plasmid was common to all resistant strains. however, all 28 tmp-smx resistant isolates exa ...	1989	2650535
bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles.	the adhesion of salmonella typhimurium to the mineral particles quartz, albite, feldspar, and magnetite was shown to correlate with the hydrophobicity of the cell surface as measured by hydrophobic interaction chromatography. the same effects were also seen for seven other selected test strains, including streptococcus faecalis, streptococcus faecium, escherichia coli, citrobacter freundii, shigella sonnei, and shigella boydii. when the test strain of salmonella typhimurium, was repeatedly culti ...	1989	2650618
[isolation of shigella boydii serotype 14 with altered biochemical characteristics in a water-borne epidemic of dysentery].	two water-borne epidemics of bacillary dysentery have been described in a garrison in which the leading causative agent has been shigella boydii, serotype of 14 altered biochemical characteristics isolated for the first time in yugoslavia. all the isolated strains of this shigella were mannitol negative and they degradated gylocose to acidity and gas already after 24 hours, and arabinose and sorbitol to acidity and gas after 48 hours. the clinical picture of the diseased was characteristic of ba ...	1989	2662591
review of cardiovascular findings in humans treated with minoxidil.	minoxidil is an antihypertensive vasodilator with no known capability of inducing human cardiovascular pathology; however, the compound does cause a late, canine-specific lesion of the right atrium and acute right atrial and ventricular papillary muscle changes. in all the autopsy cases of minoxidil-treated humans to date, 9 cases were reported with significant right atrial or other cardiac pathology and were carefully reviewed for similarity to either the early or late canine lesion. three new ...	1989	2665032
[insulin and glucocorticoid binding by blood cell receptors after hydrocortisone administration in rabbits].	repeated i.v. administration of hydrocortisone (10 mg/kg) revealed an increase in the resistance against insulin although endogenous corticosterone was decreased in 33 male rabbits. the insulin- and dexamethasone-binding receptors of erythrocytes and mononuclear leucocytes. changed after 3-7 hydrocortisone injections, the binding increasing for insulin and diminishing for dexamethasone.	1989	2680632
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
[the etiological structure of shigellosis in the ussr in recent years].	the characterization of etiological structure of shigella infection in the whole of the ussr, in individual union republics and at a number of other administrative territories of the ussr in recent years is presented. s. flexneri has been shown to prevail at the territories with unsatisfactory water supply of the population, and s. sonnei prevails at the territories with good water supply. at the former territories s. dysenteriae and s. boydii retain their etiological importance, while at the la ...	1989	2698030
primary structure and expression from complementary dna of skeletal muscle ryanodine receptor.	the sequence of 5,037 amino acids composing the ryanodine receptor from rabbit skeletal muscle sarcoplasmic reticulum has been deduced by cloning and sequencing the complementary dna. the predicted structure suggests that the calcium release channel activity resides in the c-terminal region of the receptor molecule, whereas the remaining portion constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum and the transverse tubule.	1989	2725677
terminal field of cholecystokinin-8-like immunoreactive projection neurons of the rat main olfactory bulb.	the terminal field of cholecystokinin-8 (cck)-like immunoreactive (cck-ir) tufted cells in the rat main olfactory bulb was examined by means of immunohistochemistry combined with either an anterograde tracer or a degeneration method. cck immunostaining was carried out in animals in which phaseolus vulgaris agglutinin (pha) had been injected into the main olfactory bulb. pairs of adjacent sections were processed for cck and pha immunostaining, respectively. dense cck-ir terminallike staining was ...	1989	2754048
determination of estradiol 2- and 16-alpha-hydroxylase activities in mcf-7 human breast cancer cells in culture using radiometric analysis.	using a radiometric assay the effects of estradiol upon the activity of estradiol 2- and 16 alpha-hydroxylases in mcf-7 human breast cancer cells in culture were studied. after 5 days of treatment and 36 h of withdrawal, incubation in the presence of either 2- or 16 alpha-tritiated substrate was carried out. estradiol (10 nm) significantly increased 16 alpha-hydroxylase activity (21%, p less than 0.01), while no effects on 2-hydroxylase activity was observed. treatment for 6 weeks caused a major ...	1989	2770303
opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony.		1989	2780954
results of the flow cytometry actg quality control program: analysis and findings.	the aids clinical trial group's (actg) immunology committee was charged with initiating a quality control program for all laboratories participating in the actg program reporting flow cytometry data. forty-one laboratories were evaluated. this report defines the goals of this program and the subsequent findings after 19 send-outs were made. both hiv positive volunteer donors and normal age-matched donors were used. sample sets included both heparin and edta anticoagulated bloods. laboratories we ...	1989	2785890
[effects of clopheline on impulse activity of neurons in the midbrain and dorsal horn of the spinal cord].	clophelin (1-5 mg/kg) suppresses spinal dorsal horn neuronal nociceptive responses but does not change their touch stimuli reactions in unanesthetized curarized cats. this effect is steady to naloxone (1 mg/kg), yohimbine (5 mg/kg) and removal by prazosin (1 mg/kg). clophelin depresses nociceptive responses of the central gray matter neurones which generalized pain impulses in the supraspinal structures.	1989	2804325
[effects of clopheline on impulse activity of neurons in the midbrain and dorsal horn of the spinal cord].	clophelin (1-5 mg/kg) suppresses spinal dorsal horn neuronal nociceptive responses but does not change their touch stimuli reactions in unanesthetized curarized cats. this effect is steady to naloxone (1 mg/kg), yohimbine (5 mg/kg) and removal by prazosin (1 mg/kg). clophelin depresses nociceptive responses of the central gray matter neurones which generalized pain impulses in the supraspinal structures.	1989	2804325
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
the protective effect of thromboxane synthetase inhibition on renal function in systemic sepsis.	to study the role of thromboxane in systemic sepsis and renal failure, peritonitis was induced surgically in 22 sheep, leading to local and systemic sepsis. a selective thromboxane synthetase inhibitor, u63,557a (upjohn co, kalamazoo, mi) was given before surgery in five animals and 30 minutes after surgery in five animals. a typical picture of volume-loaded, normotensive, vasodilated septic shock developed in all animals. twenty four hours after induction of sepsis, the control group showed a m ...	1989	2916566
three distinct forms of type 2a protein phosphatase in human erythrocyte cytosol.	two type 2a protein phosphatases, phosphatases i (mr = 180,000) and iii (mr = 177,000), were purified to near homogeneity from human erythrocyte cytosol. phosphatase i was composed of alpha (34 kda), beta (63 kda), and delta (74 kda) subunits in a ratio of 1:1:1. phosphatase iii comprised alpha, beta, and gamma (53 kda) subunits in the same ratio. heparin-sepharose column chromatography converted most of phosphatase i and 20% of phosphatase iii into alpha 1 beta 1 which were indistinguishable fr ...	1988	2831201
distribution and diversity of hsd genes in escherichia coli and other enteric bacteria.	we screened salmonella typhimurium, citrobacter freundii, klebsiella pneumoniae, shigella boydii, and many isolates of escherichia coli for dna sequences homologous to those encoding each of two unrelated type i restriction and modification systems (ecok and ecoa). both k- and a-related hsd genes were identified, but never both in the same strain. s. typhimurium encodes three restriction and modification systems, but its dna hybridized only to the k-specific probe which we know to identify the s ...	1988	2832380
study of plasmid screening amongst pathogenic bacteria isolated in nigeria.	clinical isolates of neisseria gonorrhoeae, campylobacter jejuni, escherichia coli, shigella dysenteriae, shigella boydii, yersinia spp. and salmonella spp. were screened for the presence of plasmids. approximately 80% of these strains harboured plasmids ranging in molecular weight from 1.0 to 45 x 10(6) daltons.	1988	2845756
relationship of free intracellular calcium to the cytolytic activity of entamoeba histolytica.	entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or n-acetyl-d-galactosamine-inhibitable surface lectin (gal/galnac adherence lectin). free intracellular ca2+ concentration [( ca2+]i) was measured in living amebae and target cells during amebic cytolysis of chinese hamster ovary (cho) cells and human polymorphonuclear neutrophils by utilizing the ca2+ probe fura-2 and computer-enhanced digit ...	1988	2897335
a selective inhibitor of camp-specific phosphodiesterase, ro 20-1724, has no effect on the quantal release of acetylcholine from the mouse phrenic nerve.	the indirect twitch response of the mouse isolated phrenic nerve-diaphragm preparation, partially paralysed by tubocurarine, was restored only by about 10% by ro 20-1724 at 2 to 280 microm. the solvent vehicle, dimethylsulphoxide, also showed the same effect to a similar extent. intracellular recordings with glass microelectrodes revealed that ro 20-1724 (40 microm) affected neither the resting membrane potential, the amplitude and frequency of miniature endplate potentials nor the amplitude of ...	1988	2897454
thermostable dipeptidase from bacillus stearothermophilus: its purification, characterization, and comparison with aminoacylase.	dipeptidase (dipeptide hydrolase [ec 3.4.13.11]) has been purified to homogeneity and crystallized from the cell extract of bacillus stearothermophilus ifo 12983. the enzyme has a molecular weight of about 86,000, and is composed of two subunits identical in molecular weight (43,000). the enzyme contains 2 g atoms of zinc per mol of protein. a variety of dipeptides consisting of glycine or only l-amino acids serve as substrates of the enzyme; km and vmax values for l-valyl-l-alanine are 0.5 mm a ...	1988	3139645
activity of glucosidase inhibitors against hiv infections.		1988	3182426
genotoxicity of carcinogens in human hepatocytes: application in hazard assessment.	evaluation of chemical genotoxicity has been used in assessing human cancer hazard, based on the observation that most human carcinogens are known to be dna-reactive. the availability of data on the dna-reactivity of compounds in metabolically competent human cells would assist hazard assessment by providing direct information of human genotoxicity. to evaluate the reliability of human hepatocytes for this purpose, the induction of dna repair by dna-reactive carcinogens of several structural cla ...	1988	3194919
depression of intestinal epithelial cell production rate by hindgut bypass in rats.	influence of hindgut bypass on the epithelial cell production rates of the small and large intestine was tested in rats. hindgut bypass decreased cryptal cell production rates of the jejunum, ileum, cecum, and distal colon by 40-65%. these results suggest that the entrance of ileal effluent into the hindgut and, presumably, the resultant microbial production of short-chain fatty acids are required to maintain the normal epithelial cell proliferation not only in the hindgut but also in the small ...	1988	3249918
effect of indomethacin on febrile response to recombinant human interleukin 1-alpha in rabbits.	effects of indomethacin, a potent inhibitor of prostaglandin (pg) synthesis, on the fever induced by recombinant human interleukin 1-alpha (rhil 1-alpha) was studied in conscious rabbits. intracerebroventricularly administered rhil 1-alpha induced a dose-dependent increase in colonic temperature that was prominently suppressed by pretreatment with indomethacin given either intracerebroventricularly or subcutaneously. on the other hand, fever induced by intravenous administration of rhil 1-alpha ...	1988	3263055
shigellosis associated with recreational water contact in los angeles county.	an outbreak of shigellosis associated with swimming at a human-made lake during the labor day weekend occurred in los angeles county in 1985. sixty-eight persons had onset of diarrheal illness within 1 week following exposure at the recreational site. thirty-three of these cases were culture-confirmed as shigellosis (29 shigella sonnei, 4 shigella boydii). fifteen persons were hospitalized. illness was highly associated with water contact, specifically swallowing water while swimming (p less tha ...	1988	3275140
quality of commercially produced shigella serogrouping and serotyping antisera.	shigella grouping antisera from five manufacturers and typing antisera from two were purchased and evaluated with homologous and heterologous shigella strains in the slide agglutination test. only 31 of 73 (42%) antisera were satisfactory. in many instances, the antisera gave negative, as opposed to weak, reactions when they should have given strong positive reactions. four reagents cross-reacted with shigella strains. of the 19 polyvalent grouping antisera to subgroups shigella dysenteriae sero ...	1988	3281972
large plasmid in shigella boydii is responsible for epithelial cell penetration.	a large plasmid in a virulent shigella boydii 5 strain was transferred to plasmid-cured avirulent strains of s. boydii 5, s. boydii 12, s. sonnei form ii, and escherichia coli k12. the transconjugants acquired the ability to invade tissue culture cells, which indicated that the large plasmid in s. boydii is responsible for epithelial cell invasiveness.	1988	3287107
[changes in the degree of sensitivity to antibiotics of shigella isolated from patients with dysentery in a city infectious disease hospital over the past 8 years].	changes in sensitivity of shigella and in particular shigella flexneri isolated from dysentery patients in a city infection hospital within 8 years i.e. from 1977 to 1985 were followed. it was shown that the changes were to the greater extent connected with preferable use of certain antibiotics in the hospital.	1988	3288150
[hoof diseases as a result of stall floor defects].	the pig's hoof is characterized by sudden changes between hard and soft horn and a high growth rate of the horn wall. these facts contribute to the high incidence of hoof alterations, which is seen in various inadequate floors. hoof alterations are only painful in cases, where the corium is irritated. some hoof alterations are described, the question of poor footing is discussed. the author provides recommendations regarding the recognition of inadequate barn floors and their improvement. stable ...	1988	3368898
trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins.	the abortifacient proteins trichosanthin, alpha-momorcharin and beta-momorcharin at nm concentrations inhibit cell-free protein synthesis. the momorcharins and the ribosome-inactivating proteins isolated from momordica charantia seeds cross-react with the respective antisera. the ribosome-inactivating proteins saporins, pokeweed antiviral protein (pap) and, to a lesser extent, gelonin have abortifacient activity on pregnant mice.	1988	3372132
transient increase of cytosolic free calcium in cultured human vascular endothelial cells by platelet-activating factor.	the effect of platelet-activating factor (paf-acether) on cytosolic free calcium, [ca2+]i, in adherent human vascular endothelial cells in culture was directly determined using a new fluorescent calcium indicator, fura-2. it was found that paf-acether but not lyso paf-acether induced a rapid and transient increase in [ca2+]i in endothelial cells. restimulation with paf-acether after the first challenge did not cause further response, while the cells were able to respond to thrombin. in the absen ...	1988	3408504
shigella boydii and shigella sonnei: serotype and drug susceptibility patterns in addis ababa, ethiopia (1974-85).		1988	3042365
congo red binding and salt aggregation as indicators of virulence in shigella species.	smooth strains of shigella dysenteriae type 1, shigella flexneri, shigella boydii, and shigella sonnei which form pigmented colonies (pcr+) on congo red agar were virulent in the sereny test. smooth variants unable to bind congo red (pcr-) were avirulent. measurements of dye uptake from solution showed that s. dysenteriae type 1 bound the most dye, followed in order of uptake by s. flexneri, s. boydii, and s. sonnei. using the salt aggregation test (sat) to determine cell surface hydrophobicity, ...	1988	3045151
[etiologic structure of bacterial dysentery in the ussr in 1983-1985].	the etiological structure of dysentery in the ussr in 1983-1985 is characterized. sonne dysentery was found to prevail in the territories with adequate water supply, while dysentery caused by shigella flexneri prevailed at the territories with unsatisfactory water supply. s. dysenteriae and s. boydii were found to play a limited role in the etiology of dysentery. in the presence of global pandemic, an increase in the isolation rate of s. dysenteriae i in the ussr is observed. the data on the bio ...	1988	3061262
kluyvera species soft tissue infection: case report and review.	a soft tissue infection caused by kluyvera species in a previously healthy woman is described. successful treatment required incision and drainage of the wound in addition to administration of antibiotics. kluyvera species are indole positive, voges-proskauer negative members of the family enterobacteriaceae. previous reports of infection due to kluyvera species suggest that the organism is never more than an opportunistic pathogen; however, we report the first case of infection in a previously ...	1988	3068255
genetic relatedness of the basic replicon of the virulence plasmid in shigellae and enteroinvasive escherichia coli.	the regions required for replication (rep) and for stable maintenance (stb) of the 230 kilobase virulence plasmid, pmysh6000, in shigella flexneri 2a ysh6000 were identified on the restriction enzyme sa/l fragments, c and o, respectively. the stable plasmid, pmysh6610, consisting of adjacent fragments c and o was found to belong to the incompatibility group fl. in all strains of shigellae and enteroinvasive escherichia coli (eiec) tested, virulence plasmids were found to be highly homologous wit ...	1988	3070262
[shigella serotypes and drug resistance patterns of strains isolated in etimesgut, turkey, in 1987].	drug resistance patterns of shigella strains were investigated in a prospective manner in etimesgut district during a period of 1 year. thirty strains shigella were isolated, belonged to three subgroups with preponderance of sh. flexneri (70%), followed by sh. sonnei (27%) and sh. boydii (3%). the resistance was highest with streptomycin (80%), followed by trimethoprim-sulfamethoxazole (tmp-smz) (53%) and ampicillin (43%). only three strains (10%) were sensitive to all eight antibiotics tested. ...	1988	3078794
[improved method of lipopolysaccharide isolation from gram-negative bacteria].	a modification of the traditional method for lipoplysaccharide isolation from the cells of grammnegative bacteria was elaborated on the basis of extraction by the hot water solution of phenol (the method of westfahl). to make the method simpler and to raise the yield of the product it was proposed to use the water-phenol extract without its division for plases. the nucleic acids are eliminated by precipitation from dialyzed extract at ph 3,2-3,4 achieved by addition of acetic acid. the comparati ...	1987	3112564
specificity of membrane complement receptor type three (cr3) for beta-glucans.	the binding of the ic3b receptor (cr3) to unopsonized zymosan was shown to result from cr3 attachment to cell wall beta-glucans. a specificity of neutrophil responses for beta-glucan was first suggested by a comparison of yeast (saccharomyces cerevisiae) cell wall components for stimulation of a neutrophil superoxide burst. neutrophils responded poorly to heat-killed yeast, but gave increasingly better responses to cell wall polysaccharides devoid of proteins (zymosan) and nearly pure beta-gluca ...	1987	3040332
liver cell lines.		1987	3431441
serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria.	188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus.	1987	3443752
residual nitrobenzylthioinosine-resistant nucleoside transport in a transport mutant (ae1) of s49 murine t-lymphoma cells.	the uptake of various nucleosides by s49 mouse t-lymphoma cells and that by a single-step nucleoside transport-defective mutant thereof (ae1) were compared. residual nucleoside entry into ae1 cells occurred via two routes, nonmediated permeation and saturable, non-concentrative transport with broad substrate specificity and a michaelis-menten constant approximating that for thymidine transport in wild-type cells. however, in contrast to nucleoside transport in wild-type cells, residual nucleosid ...	1987	3494190
dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen.	the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ...	1987	3495738
effects of marine mammal parasites on human health.		1987	3294653
an accessory protein identical to mouse egasyn is complexed with rat microsomal beta-glucuronidase and is identical to rat esterase-3.	we report biochemical, immunological, and genetic studies which demonstrate that an accessory protein with the essential features of mouse egasyn is complexed with and stabilizes a portion of beta-glucuronidase in microsomes of rat liver. the accessory protein exists as a complex with beta-glucuronidase since it coprecipitates with beta-glucuronidase after treatment of extracts with a specific beta-glucuronidase antibody. the two proteins are associated by noncovalent bonds since they are easily ...	1987	3294829
epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats.	the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ...	1987	3298192
clinical presentation of shigellosis during the 1984 epidemic of bacillary dysentery in west bengal.		1987	3301795
[a reservoir of shigella of avian origin: herons and birds of prey of the zoological garden in tananarive].	the authors record the bacteriological characters of four shigella strains: s. dysenteriae 2 and s. boydii 9, respectively isolated in a bird-of-prey and in three herons, kept in the zoological garden in antananarivo. the significance of this malgasian avian reservoir of shigella is discussed.	1987	3304678
a new chromogenic test for the detection of urokinase in the genus shigella.	a simple test for detection of urokinase in shigella is described. the test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mm solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (chromozym u). enzymatic activity is revealed by formation of a yellow colour after 24 h of incubation at 37 degrees c. the test is able to differentiate serotypes of shigella dysenteriae, shigella flexneri, shigella boydii. the results suggest the possibility of incl ...	1987	3306294
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.	enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...	1987	3312015
bactericidal activities of selected organic n-halamines.	the bactericidal efficacies of three organic n,n'-dihalamine disinfectants in the class of compounds termed imidazolidinones were determined for combinations of ph, temperature, and water quality treatments by using staphylococcus aureus and shigella boydii as test organisms. the compound 1,3-dibromo-4,4,5,5-tetramethyl-2-imidazolidinone was found to be the most rapidly acting bactericide, especially under halogen-demand-free conditions. the mixed n,n'-dihalamine 1-bromo-3-chloro-4,4,5,5-tetrame ...	1987	3314705
shigella bacteremia in adults. a report of five cases and review of the literature.	shigella bacteremia is rare, occurring mainly in children. we describe five adult patients with shigella bacteremia and review data on 22 cases reported in the english-language medical literature. eighteen (67%) of 27 patients had either an underlying condition or were aged older than 65 years. most patients had clinical signs of acute febrile gastroenteritis. however, in six patients, the organism was not isolated from stool. species isolated from blood included shigella flexneri in 11 patients ...	1987	3314766
[medico-genetic studies of human provisional organs].		1987	3318529
novel oral vaccines: the effectiveness of cloned gene products on inducing secretory immune responses.		1987	3321939
differential clinical features and stool findings in shigellosis and amoebic dysentery.	to obtain information that could assist the clinician to differentiate between shigellosis and amoebic dysentery, we compared clinical features and stool findings in 58 adult male patients in bangladesh. mean values indicated that patients with invasive amoebiasis were older and had a longer prehospital illness, a lower body weight, less frequent fever, a lower haematocrit and a higher white blood cell count than patients with shigellosis. the mean number of faecal leucocytes per mm3 was signifi ...	1987	3328339
convulsions in childhood shigellosis. clinical and laboratory features in 153 children.	we studied 153 children who experienced convulsions associated with shigellosis. the male-female ratio was 1.2:1.0. thirty-six children had a previous history of febrile convulsions, and 31 children had a family history of convulsive disorder. most of the children were 0.5 to 3 years of age, although 49 (32%) were older than 3 years of age and 20 (13.1%) were older than 5 years of age. all children were febrile; in 75% of the children, the temperature was over 39 degrees c. the majority of the c ...	1987	3544808
voluntary hyperventilation changes recruitment order of parasternal intercostal motor units.	the order of recruitment of single-motor units in parasternal intercostal muscles during inspiration was studied in normal human subjects during quiet breathing and voluntary hyperventilation. electromyograms were recorded from the second and third intercostal spaces by means of bipolar fine wire electrodes. flow at the mouth, volume, end-expired co2, and rib cage and abdominal anterior-posterior diameters were monitored. single-motor units were identified using criteria of amplitude and shape, ...	1987	3558179
[proliferative activity of experimental brain tumors].	the method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. the tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was ...	1987	3564792