Publications

isolation and mapping of a polymorphic dna sequence (pynz32) on chromosome 4p [d4s125].		1988	2897672
effect of atrial peptide on collecting duct function.	1. the effects of synthetic rat atrial natriuretic peptides (anp) on diffusional 22na+, 36cl- and tritiated water (tho) permeability of in vitro microperfused rat papillary collecting ducts and the effect in vivo of anp on stop-flow sodium concentrations in the terminal segment of rabbit nephrons were studied. 2. the addition of 4 x 10(-8) or 4 x 10(-7) mol/l anp to the medium or perfusion solution did not alter diffusional 22na+ or 36cl- permeability of microperfused rat papillary collecting du ...	1988	2976327
facilitatory action of adrenergic drugs on muscle twitch evoked by nerve stimulation in the curarized rat phrenic hemidiaphragm.	the facilitatory effects of alpha-adrenergic drugs on neuromuscular transmission has been studied in the curarized rat phrenic-hemidiaphragm. the reversal effect of blocking action of (+)-tubocurarine on indirectly-induced twitch by alpha-adrenergic agents was higher with noradrenaline (ec50 = 6.51 +/- 0.18 microm) compared with adrenaline (ec50 = 11.80 +/- 1.06 microm) or phenylephrine (ec50 = 17.36 +/- 1.02 microm). methoxamine, clonidine and azepexol showed no effect. the action of noradrenal ...	1988	3421779
lactational response to soybean meal, heated soybean meal, and extruded soybeans with ruminally protected methionine.	seventy-three high producing holstein cows were arranged in a 3 x 2 factorial to evaluate three protein supplements (soybean meal, heat-treated soybean meal, and extruded blend of soybeans and soybean meal) without or with 15 g/head/d of ruminally protected dl-methionine during wk 4 through 16 postpartum. total mixed diets contained (dm basis) 30% corn silage, 15% alfalfa hay, and 55% of the respective concentrate mix. milk production was higher when cows were fed either heated soybean product i ...	1988	3372801
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase].		1988	3395180
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
enoxacin: in-vitro and animal evaluation as a parenteral and oral agent against hospital bacterial isolates.	enoxacin was evaluated in in-vitro tests and in studies of effectiveness and blood concentrations in the mouse. enoxacin was active against both susceptible and multiresistant hospital isolates of enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae, neisseria gonorrhoeae and staphylococci. less susceptible were streptococci and anaerobes. of nine quinolones tested, only norfloxacin was equivalent in vitro. the mbcs of enoxacin were one- to twofold greater than the mics, and enoxac ...	1988	3129392
development of gentamicin resistance in gram-negative bacteria in czechoslovakia and correlation with its usage.	widespread use of gentamicin in czechoslovakia began in 1975 when it became more available, although its use remained restricted until 1986. starting in 1980 a remarkable increase in gentamicin resistance was observed in pseudomonas aeruginosa, klebsiella, enterobacter and several proteus species, especially proteus rettgeri. a large proportion of gentamicin-resistant strains came from the urine of hospitalized patients. in spite of infection control measures and restrictive antibiotic policy, g ...	1988	2854783
[urine bacterial flora and chemoantibiotic resistance: observations on ambulatory patients and hospitalized patients at the local health district no. 11 of fabriano (ancona)].	this paper reports the results of a study carried out on 4614 samples of urine from inpatients and outpatients according to the sex. the positive monomicrobic urine specimens were examined and isolated strains were tested against various antimicrobial drugs. our findings showed that 43.1% of urine specimens examined from inpatients and 38.4% from outpatients proved to be positive. among the bacterial species more frequently isolated, significant differences among enterobacter spp. in males and c ...	1988	3268917
isolation of flagella from the archaebacterium methanococcus voltae by phase separation with triton x-114.	the flagella of methanococcus voltae were isolated by using three procedures. initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in kbr gradients. flagella were also prepared by solubilization of cells with 1% (vol/vol) triton x-100 and purified as described above. both of these techniques resulted in variable recovery and poor yield of flagellar filaments. purification of intact flagella (filament, hook, and basal body) was ach ...	1988	3127380
immunocytochemical delineation of thyrotrophic area: origin of thyrotropin-releasing hormone in the median eminence.	to identify the specific thyrotropin-releasing hormone (trh)-containing neurons projecting to the median eminence (me), a retrograde tracing method with horseradish peroxidase (hrp) was combined with immunocytochemical staining for trh. three days after hrp injection restricted to the me, several trh-positive neuronal perikarya were found to contain hrp. such double-stained cells were exclusively distributed in the anterior parts of the periventricular nucleus and the most medial parts of the pa ...	1988	3135505
mechanism of inhibition of chromosomal beta-lactamases by third-generation cephalosporins.	the kinetic interactions of the beta-lactamase from enterobacter cloacae 908 r with ceftriaxone, cefotaxime, and ceftazidime have been examined in detail. with all of these cephalosporins, there is an initial rapid reaction involving opening of the beta-lactam that then decreases to a slower steady-state rate (kss) of beta-lactam hydrolysis (at 37 degrees c: ceftriaxone, kss = 0.044 s-1; cefotaxime, kss = 0.033 s-1; ceftazidime, kss = 0.011 s-1). more than stoichiometric quantities of beta-lacta ...	1988	3142012
studies on the host/parasite interface during the development of a pentastomid arthropod parasite in rodent intermediate hosts, with observations on protective surface membranes.	the changing structure of the cuticle of the arthropod pentastomid parasite porocephalus crotali, during growth to the infective stage in mouse and rattlesnake hosts, is described. the outermost cuticulin layer of the cuticle in instars ii-vi is elevated to form a dense mat of epicuticular hairs. since the vi larval cuticle is retained by the infective (vii) nymph as a protective sheath, effectively all stages in mice present a hairy surface to the host and this may constitute a physical barrier ...	1988	3232142
the effects of clavulanic acid and sulbactam on beta-lactamase biosynthesis.	clavulanic acid and sulbactam were tested as inducers of beta-lactamase in 21 strains of gram-negative bacteria. beta-lactamase synthesis was inducible in all these strains, as demonstrated by the increased enzyme activities obtained when cells were grown in the presence of 10 mg/l of cefoxitin, the increase varying from 11- to 734-fold however, at the same concentration, neither inhibitor induced significant amounts of beta-lactamase, except in one strain of providencia rettgeri, where both wer ...	1988	3263350
in vitro and in vivo antibacterial activities of me1207, a new oral cephalosporin.	me1207 (pivaloyloxymethyl ester of me1206) is a new oral cephalosporin. me1206 is (6r,7r)-7-[(z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)- acetamido]-3-[(z)-2-(4-methylthiazol-5-yl)-ethyl]-cephem-4-carboxy lic acid. the susceptibilities of about 1,600 clinical isolates to me1206 were determined by the agar dilution method. me1206 showed a broad spectrum of activity against gram-positive and gram-negative bacteria. me1206 was more active than cefaclor, t-2525, and cefixime against staphylococcus ...	1988	3264132
contamination of freshly slaughtered pig carcasses with human pathogenic yersinia enterocolitica.	evidence is presented for the extent of contamination of freshly slaughtered pig carscasses with human pathogenic yersinia enterocolitica and shows the significance of faecal contamination as a source of infection. swab samples collected from the rectum and the surface of a total of 1458 pig carcasses were examined for the presence of human pathogenic y. enterocolitica. y. enterocolitica, biovar iv, serogroup 0:3, were isolated from the rectum of 360 pigs (24.7%). the organism was isolated from ...	1988	3275322
regulation of ovarian cell growth through the local production of transforming growth factor-alpha by theca cells.	the rapid proliferation of a tissue often requires the local production of a specific growth factor. the ovarian follicle is a rapidly growing tissue which contains two primary somatic cell types, granulosa cells and theca cells. theca cells and granulosa cells were isolated from bovine ovaries and cultured to assess the possible local production of a growth factor within the ovarian follicle. serum-free conditioned medium from theca cells, but not from granulosa cells, was found to contain a co ...	1988	3264238
the unborn patient.		1988	3347563
commercial identification systems often fail to identify providencia stuartii.	we tested 145 clinical isolates in an attempt to evaluate some of the most widely used commercial identification systems in europe in terms of their ability to identify providencia strains. two manual miniaturized systems (api 20e and enterotube ii) and three mechanized-automated systems (cobas-bact, sceptor system, and titertek-enterobac-rapid automated system) were evaluated. providencia alcalifaciens and providencia rettgeri strains were correctly identified by all systems in all cases, and i ...	1988	3277997
[meningoencephalitis and mononucleosis syndrome associated with acute infection by human immunodeficiency virus].		1988	3347109
ureterocolonic anastomosis in clinically normal dogs.	ureterocolonic anastomosis was evaluated in 13 clinically normal dogs. urinary continence was maintained after surgery, and the procedure was completed without technique errors in all but 2 dogs. three dogs died within 5 weeks (2 of undetermined causes and 1 of aspiration pneumonia and neurologic disease), and 1 dog was euthanatized 4 months after surgery because of neurologic signs. two healthy dogs were euthanatized 3 months after surgery for light microscopic evaluation of their kidneys. five ...	1988	3421537
cardiac effects of botulinal toxin.	crystalline type a botulinal toxin rapidly caused temporary bradycardia and electrocardiographic (ecg) changes in mice, rats, rabbits and dogs. in addition, in the dog the force of contraction was measured and found to be depressed. the ecg changes were indicative of conduction defects. the hemagglutinin present in the toxin played no role in the effects on the heart, since a derivative toxin without hemagglutinin also caused these phenomena. the cardiac effects were spontaneously reversible in ...	1988	3421785
[nationwide study of the antimicrobial susceptibility of clinical isolates of proteus group in japan].	we discussed the antimicrobial susceptibilities of proteus group isolated in 1983 and 1984 and also the annual changes of the susceptibilities from 1980 to 1984. the tested strains were isolated in 103 hospitals in japan. antibiotics tested for this study were ampicillin (abpc), cefazolin (cez), cefmetazole (cmz), and gentamicin (gm). the mic's were determined by the standard method of the japan society of chemotherapy. susceptibilities of the bacterial strains to the 4 antibiotics were describe ...	1987	3475483
[drug resistance of proteus vulgaris, proteus morganii and providencia rettgeri isolated from diagnostic specimens].		1987	3324173
[the antimicrobial activity of sisomicin against fresh clinical isolates].	antimicrobial activities of sisomicin (siso) against clinical isolates obtained in the second half of 1986 were investigated together with other 4 aminoglycosides (ags) (gentamicin (gm), tobramycin (tob), dibekacin (dkb), amikacin (amk] and 2 cephems (cefotiam, cefotaxime), and were compared to the results reported in the period of late 1970's through early 1980's in japan. 1. the incidence of siso-resistant staphylococcus aureus in the present study was 18% and is comparable to that of the othe ...	1987	3327965
investigation of a providencia rettgeri strain that has enterobacterial common antigen in the immunogenic state.	antigenic material obtained by phenol-water extraction from providencia rettgeri strains, escherichia coli o:14 strains, and mutants of the e. coli o:14 strain were examined by the passive (indirect) hemagglutination technique, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and by immune blotting (lipopolysaccharide (lps) blotting). providencia rettgeri 965, like e. coli o:14, was demonstrated to have an enterobacterial common antigen (eca) in the immunogenic form but, unlike e. c ...	1987	3328641
in vitro and in vivo antibacterial activities of cs-807, a new oral cephalosporin.	cs-807 is a new oral prodrug of r-3746, a cephalosporin derivative, with potent in vitro and in vivo antibacterial activity against both gram-positive and gram-negative bacteria. the susceptibility of about 1,200 clinical isolates to r-3746 was determined by the agar dilution method. ninety percent or more of pathogens such as staphylococcus aureus, streptococci, escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, indole-positive and indole-negative proteus spp., providencia rettgeri, a ...	1987	3310868
identification of porins in outer membrane of proteus, morganella, and providencia spp. and their role in outer membrane permeation of beta-lactams.	proteus mirabilis, proteus vulgaris, morganella morganii, providencia rettgeri, and providencia alcalifaciens, which were once classified into the same genus, proteus, were studied. cefoxitin-resistant mutants from these species were isolated, and it was confirmed that the resistance was attributed to the lack of an outer membrane protein, resulting in a significant decrease in the penetration of hydrophilic cephalosporins through the outer membrane. comparison of the mutant strains with their p ...	1987	3034144
microbiological perspectives of co-trimoxazole.	trimethoprim-sulfamethoxazole in vitro activity was compared with ampicillin, tetracycline, sulfonamide and trimethoprim against isolates of 24 gram-negative and 11 gram-positive species. the incidence of more than 10% of strains with minimal inhibitory concentrations above 32 mg/l was restricted to escherichia coli, shigella spp., klebsiella pneumoniae, providencia rettgeri, morganella morganii, methicillin-resistant staphylococcus aureus and staphylococcus epidermidis. occasionally, streptococ ...	1987	3325434
alteration of trichostrongylus colubriformis egg permeability by bacillus thuringiensis israelensis toxin.	a toxin from the bacterium bacillus thuringiensis israelensis is lethal to nematode eggs. exposure of eggs of the ruminant nematode trichostrongylus colubriformis to the toxin significantly increased the eggs' permeability to radiolabeled phenylalanine within 2 hr. calcium chloride inhibited the toxin-induced change in egg permeability. iodine staining of eggs that were exposed to the microbial toxin revealed that egg permeability was altered within 5 min and was dependent on the dose of toxin. ...	1987	3295166
in vitro activity and beta-lactamase stability of a new monobactam, b0-1165.	b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ...	1987	3300528
acute gangrene of the scrotum and penis in four hematologic patients. the usefulness of hyperbaric oxygen therapy in one case.	we describe four cases of fournier's gangrene complicating the postchemotherapy aplastic phase (polymorphonuclear cells [pmn] less than 500/microliter) in three patients with acute leukemia and one patient with non-hodgkin's lymphoma. blood and local cultures from two patients contained pseudomonas aeruginosa, and local cultures from another patient grew both p. aeruginosa and proteus rettgeri. early recognition and aggressive antibiotic and surgical treatment with granulocyte recovery were fund ...	1987	3113713
[regulation of milk intake in young ruminants].	we studied the influence of the three main nutritive components of milk - saccharides, fat and amino acids - on milk intake in suckling lambs and on the role of insulin as a key metabolic hormone for the regulation of milk intake. the intake was slowed down after p.o. administration of milk fat (p less than 0.05), after i. v. administration of methionine (p less than 0.001) and after p. o. administration of glucose, lactose (p less than 0.01, p less than 0.001) and sorbitol (p less than 0.001). ...	1987	3109104
heterologous protection in murine cutaneous leishmaniasis.	mice immunized with a glycolipid antigen (gl) of leishmania major plus adjuvant are relatively resistant to subsequent infection with this protozoan parasite. the gl is affinity purified on the monoclonal antibody wic-79.3 which is l. major-specific and does not react with l. donovani. when another monoclonal, wic-108.3, which cross-reacts with several leishmania species, is used to affinity purify gl from l. donovani, the eluted material can partially protect genetically resistant mice against ...	1987	3436638
in vitro study of the antibacterial activity of ofloxacin against recent clinical isolates.	the in vitro activity of ofloxacin, a new broad-spectrum antimicrobial agent, was studied by a standardized single disc method. a total of 990 clinical isolates were tested, including 20 strains of anaerobic bacteria. ofloxacin was highly active against 683 strains (70.41%), had intermediate activity against 109 (11.23%) and had no activity against 178 (18.35%). ofloxacin was highly active against e. coli, klebsiella sp., citrobacter sp., proteus mirabilis, proteus morganii, salmonella sp., camp ...	1987	3476290
in vitro activity and beta-lactamase stability of a new penem, cgp 31608.	the in vitro activity of cgp 31608, a new penem, against aerobic and anaerobic organisms was evaluated and compared with those of other beta-lactams. cgp 31608 inhibited escherichia coli, klebsiella pneumoniae, k. oxytoca, proteus mirabilis, citrobacter diversus, and salmonella, shigella, aeromonas, and yersinia spp. with mics for 50% of the strains (mic50s) of 2 to 4 micrograms/ml and mic90s of 4 micrograms/ml, compared with cefotaxime, ceftazidime, aztreonam, and imipenem mics of less than 0.2 ...	1987	3496845
comparative activity of ceftizoxime against aminoglycoside-resistant aerobic gram-negative bacilli.	the in vitro activity of ceftizoxime was compared with that of other beta-lactam antibiotics against 331 aminoglycoside (ag)-resistant clinical isolates. two hundred and six ag-resistant, beta-lactamase producing, r-plasmid harbouring enterobacteriaceae strains had mics ranging from 0.0125 to 0.063 mg/l. ag-resistant escherichia coli (36 strains) and klebsiella pneumoniae (19) had mic 90 values of 8 mg/l. proteus rettgeri and p. vulgaris as well as morganella morganii, resistant to several ags, ...	1987	3497792
[age-related changes in lectin binding patterns of rat cartilaginous tissues].		1987	3505808
[age-related changes in lectin binding patterns of rat cartilaginous tissues].		1987	3505808
effects of peptide yy on gallbladder motility.	the effects of peptide yy (pyy) on cholecystokinin-stimulated gallbladder contraction were investigated in the prairie dog model. twelve animals underwent laparotomy with catheter placement into the gallbladder and common bile duct (vent). the gallbladder was continuously perfused with [14c]polyethylene glycol-labeled lactated ringer at 0.03 ml/min, and vent effluent was collected at 2.5-min intervals. all animals received 20 min of intravenous infusion of cholecystokinin octapeptide (cck-op), 2 ...	1987	3591942
genetic and biochemical diversity of ureases of proteus, providencia, and morganella species isolated from urinary tract infection.	bacterial urease, particularly from proteus mirabilis, has been implicated as a contributing factor in the formation of urinary and kidney stones, obstruction of urinary catheters, and pyelonephritis. weekly urine specimens (n = 1,135) from 32 patients, residing at two chronic-care facilities, with urinary catheters in place for greater than or equal to 30 days yielded 5,088 phenotypically and serotypically diverse bacterial isolates at greater than or equal to 10(5) cfu/ml. a total of 86% of sp ...	1987	3623698
[high mortality of the nile tilapia oreochromis niloticus caused by providencia rettgeri].		1987	3632601
the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei.	fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ...	1987	3554006
effect of flutamide, a nonsteroidal antiandrogen on testis and accessory genital organs of male rats: a reevaluation in prepubertal and adult rat.		1987	3666808
[primary leiomyosarcoma of the pulmonary artery. apropos of a case].	a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ...	1987	3122693
covalent coupling of 4-thiouridine in the initiator methionine trna to specific lysine residues in escherichia coli methionyl-trna synthetase.	a new method has been developed to couple a lysine-reactive cross-linker to the 4-thiouridine residue at position 8 in the primary structure of the escherichia coli initiator methionine trna (trnafmet). incubation of the affinity-labeling trnafmet derivative with e. coli methionyl-trna synthetase (metrs) yielded a covalent complex of the protein and nucleic acid and resulted in loss of amino acid acceptor activity of the enzyme. a stoichiometric relationship (1:1) was observed between the amount ...	1987	3122828
letter: liver tumours and the pill.		1987	174777
[new protein-free film method for electron microscopy of dna].		1987	174876
ferric ammonium citrate decomposition--a taxonomic tool for gram-negative bacteria.	the iron uptake test of szabó and vandra has been modified and used for the differentiation of gram-negative bacteria. nutrient agar containing 20 g per litre of ferric ammonium citrate was distributed into narrow tubes and solidified so as to form butts and slants. considering the localization of the rusty-brown coloration produced after seeding and incubation, 2367 strains were classified into four groups. (1) unchanged medium: escherichia coli, shigella spp., yersinia spp., hafnia alvei and m ...	1986	3529797
the association of acrylamide with proteins. the interpretation of fluorescence quenching experiments.	the association properties of acrylamide with a number of proteins in aqueous solution have been investigated by a fluorescence-quenching method previously used in micelles and lipid bilayers (blatt, e., chatelier, r.c. and sawyer, w.h. (1984) chem. phys. lett. 108, 397-400). at ph 7.0, acrylamide partitions between the bulk aqueous phase and the proteins, human serum albumin, monellin and ovalbumin. comparison with an earlier method of analysis (sikaris, k.a., thulborn, k.a. and sawyer, w.h. (1 ...	1986	3697370
rat cns neurons are not yet programmed to shorten their chromatin repeat length at the end of fetal neurogenesis.	neurons from rat fetal cerebral hemispheres were grown in a synthetic medium (maat medium), as previously described, for different periods of time. the repeat length of their chromatin was determined by micrococcal nuclease digestion and compared with that of neurons isolated from postnatal rat brain of corresponding ages. in contrast to the in vivo situation, we found that neurons, dissociated at the 16th gestational day and cultured in vitro, did not undergo the shortening of their chromatin r ...	1986	3719702
[activation of protein c on the surface of cultivated endothelial cells--role of antithrombin iii].		1986	3811501
spinal cord serotonin release and raised blood pressure after brainstem kainic acid injection.	the recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. in the present experiments we have shown that chemical stimulation of bulbospinal neurons in the region of the b3 cell group in the ventromedial medulla, causes an increase in the release of serotonin in the thoracic spinal cord and that this ...	1986	3697690
unique spectral peak in phrenic nerve activity characterizes gasps in decerebrate cats.	the respiratory pattern of gasping has been characterized on the phrenic nerve as rapidonset, rapid-rise, large-amplitude bursts of neural activity. furthermore, medullary sites critical for the neurogenesis of gasping have been identified and are not the sites of identified respiratory neurons, such as the dorsal and ventral respiratory groups. i classified envelopes of phrenic nerve activity as eupneic breaths, or gasps based on the time-domain features of duration, shape, and amplitude. gasps ...	1986	3957831
antibacterial interaction of cefuroxime and serum from uraemic patients.	a strain of proteus rettgeri was exposed to combinations of subinhibitory concentrations of cefuroxime and serum from normal subjects and from uraemic patients (on conservative and on haemodialysis treatment) and the antibacterial activity of these was determined. cefuroxime and normal serum gave a synergistic effect. compared with controls, the combination of cefuroxime and uraemic serum from patients on conservative treatment showed a reduced inhibitory effect. the antibacterial activity of th ...	1986	3771426
[effect of splenin on the concentration of biogenic amines in the tissues and blood plasma of the rat].		1986	3721001
neplanocin a. actions on s-adenosylhomocysteine hydrolase and on hormone synthesis by gh4c1 cells.	we have investigated the biochemical actions of neplanocin a (nepl a), a carbocyclic adenosine analog, on purified calf liver s-adenosylhomocysteine hydrolase and in the gh4c1 strain of functional rat pituitary cells. addition of 1 mol of nepl a/2 mol of s-adenosylhomocysteine hydrolase subunit led to rapid and complete inactivation. concomitant with inactivation, half of the enzyme-bound nad was reduced and adenine was released stoichiometrically from nepl a. in gh4c1 cells nepl a caused a dose ...	1986	3957905
tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor.	tumor necrosis factor type alpha (tnf-alpha) is produced by monocytes and has been purified, sequenced, and cloned from the hl-60 cell line. soluble products of monocytes stimulate endothelial cells to release multilineage hematopoietic colony-stimulating activity. to determine whether tnf-alpha could stimulate endothelial cells to produce these activities, we added recombinant human tnf-alpha to cultured human umbilical vein endothelial cells. untreated endothelial cell conditioned medium and t ...	1986	3489939
sequence of myosin-crossreactive epitopes of streptococcal m protein.	group a streptococcal m proteins contain epitopes that crossreact with sarcolemmal membrane proteins of human myocardium and myosin. in the present study, synthetic peptide copies spanning the entire 197-residue pepsin extracted fragment of type 5 m protein were used to localize the myosin-crossreactive epitopes of the molecule. peptide 84-116 inhibited by 75% the binding of myosin-crossreactive antibodies evoked by pep m5, as determined by elisa. immunoblot inhibition studies confirmed that pep ...	1986	2430047
in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics.	a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ...	1986	3088346
in vitro evaluation of a-56619 (difloxacin) and a-56620: new aryl-fluoroquinolones.	the in vitro antibacterial potencies of a-56619 and a-56620, two new aryl-fluoroquinolones, were compared with the potency of norfloxacin against a broad spectrum of organisms. cefotaxime, aztreonam, piperacillin, imipenem, penicillin, and gentamicin were also tested for reference purposes. the mics required to inhibit at least 90% of the strains tested ranged from 0.25 to 4 micrograms/ml for a-56619 and from 0.06 to 0.5 microgram/ml for a-56620 for members of the enterobacteriaceae. a-56619 was ...	1986	3087274
magnesium-dependent plaque formation by bacteriophage p1cinc(-) on escherichia coli c and shigella sonnei.	phage p1c(-), in a state of the phage not infective to escherichia coli k12, was able to form plaques on a wild-type strain of e. coli c and on shigella sonnei in the presence of mg2+. citrobacter freundii, enterobacter aerogenes, and a salmonella typhimurium gale mutant were not lysed by, but were lysogenized with p1cinc(-), whereas klebsiella pneumoniae, proteus rettgeri, and s. typhimurium lt2 were not susceptible to either p1cinc(-) or p1cinc(+). the lipopolysaccharide structure of e. coli c ...	1986	3535235
[mutants of escherichia coli k-12 with increased resistance to ionizing radiation. vi. increased radioresistance and heat shock proteins].	by means of one-dimensional electrophoresis, it is shown that in radiation-resistant gamr444 and gamr445 mutants of escherichia coli k-12 high-molecular weight heat shock proteins are hyperproduced at 32-37 degrees c and are induced more intensively during heat shock (in comparison to the parental wild-type strain ab1157). when the missense htpr15 mutation of the positive regulatory htpr gene for heat shock proteins was introduced by transduction into the genome of the gamr444 mutant, its enhanc ...	1986	3542709
microbiological basis for the use of fosfomycin trometamol as single-dose therapy for simple cystitis.	fosfomycin trometamol (fot), a new soluble salt of fosfomycin, was developed especially for single-dose treatment in uncomplicated urinary tract infections. in this study, the minimum inhibitory concentrations (mics) of fot were measured both in nutrient broth and human urine and compared with calcium fosfomycin, pipemidic acid and cotrimoxazole. a total of 300 bacterial strains of different species from recent urinary infections were studied. staphylococcus aureus showed the lowest mic (0.38 mi ...	1986	3094965
[effect of immobilization stress on the course of experimental influenza infection and the process of interferon formation].	the experimental data on the peculiar features of interferon production in c57bl/6 mice, infected with the lethal dose of influenza virus and simultaneously subjected to the action of a stress factor, are presented. immobilization stress was found to exert pronounced influence on the interferon-producing system of the body, which was manifested by the appearance of alpha interferon in a titer of up to 1:80 in the blood of intact animals. 6-hour immobilization preceding infection did not accelera ...	1986	3705812
[frequency of isolation and drug resistance of bacterial strains from urine samples tested in the bacteriology and virology laboratory of the u.s.l. 10/d in florence].	two thousand five hundred and sixty-eight positive monomicrobic urine samples (from general practice and hospital wards) were examined and isolated strains were tested against various antimicrobial drugs. our findings showed that urinary-tract infections caused by escherichia coli were the most common in females, whereas those by pseudomonas aeruginosa, proteus rettgeri, enterobacter species, other difficult gram-negative rods and streptococcus faecalis were in total the most common in males. if ...	1986	3786667
disappearance and effects of exogenous lipid transfer activity in rats.	these studies were performed to determine the role of plasma lipid transfer activity in the regulation of plasma total and lipoprotein cholesterol in vivo. partially purified human lipid transfer activity was injected into rats at a level similar to that of normal rabbit plasma, d greater than 1.21. the disappearance of exogenous lipid transfer activity from rat plasma was biphasic, with a 70% loss within 6 h. the remaining 30% was lost with a half-time of about 14 h. in the rat, short-term expo ...	1986	3768397
analysis of factors involved in determining urinary bladder voiding cycle in urethan-anesthetized rats.	the mechanism(s) involved in determining the voiding cycle of the rat urinary bladder have been investigated in urethan-anesthetized animals. fluid emission is almost confined to that phase of the voiding cycle which is characterized by the presence of a series of high-frequency oscillations in intraluminal pressure (iphfo). during this phase the mean urethral flow rate reached a maximum and fluid was expelled in a stream-like fashion. the index obtained by multiplying the amplitude of iphfo by ...	1986	3740305
synergy of fosfomycin with beta-lactam antibiotics against staphylococci and aerobic gram-negative bacilli.	fosfomycin inhibits bacterial cell wall synthesis at the initial stage. it can act synergistically with beta-lactams. the effect of the combination of fosfomycin and selected penicillins and cephalosporins against staphylococci, pseudomonas aeruginosa, pseudomonas cepacia and selected gram-negative bacteria was determined. synergy was determined by agar dilution and checkerboard titration methods; synergy was defined as an fic index less than or equal to 0.5 and partial synergy greater than 0.5 ...	1986	3569007
[the effect of subinhibitory concentrations of cefaclor, erythromycin and doxycycline on phagocytosis and intracellular killing of e. coli by human neutrophil granulocytes].	after determination of granulocyte basic parameters of polymorphonuclear neutrophilic leukocytes obtained from 5 unselected intensive care unit patients and 5 healthy volunteers phagocytosis of an e. coli strain isolated from clinically proven infection (urinary tract infection) was investigated with and without preincubation of the strain with subinhibitory concentrations (1/4 and 1/2 minimum inhibitory concentration; incubation time: 30 min) of cefaclor, erythromycin and doxycycline. the resul ...	1986	3545226
haemophilus influenzae type b colonization in household contacts of infected and colonized children enrolled in day care.	strategies for management of children attending day-care facilities after a case of haemophilus influenzae type b disease are controversial. the success of chemoprophylaxis in preventing subsequent cases has been variable. failure of rifampin prophylaxis as currently recommended may result from usage limited to direct contacts of the index patient. this prospective study was designed to ascertain the extent of colonization in household contacts of colonized children attending day-care facilities ...	1986	3487771
lipopolysaccharide of providencia rettgeri. chemical studies and taxonomical implications.	the chemical constitutional analysis of the lipopolysaccharide (lps) isolated from providencia rettgeri was carried out. polyacrylamide gel electrophoresis using sodium dodecylsulfate or sodium deoxycholate showed that the lipopolysaccharide mostly consisted of short sugar chains. the lipid a was precipitated out after mild acid hydrolysis of lps. from the supernatant degraded polysaccharide and unsubstituted core fractions were isolated. compositional analysis of the core material revealed the ...	1986	3524498
expression and regulation of the penicillin g acylase gene from proteus rettgeri cloned in escherichia coli.	the penicillin g acylase genes from the proteus rettgeri wild type and from a hyperproducing mutant which is resistant to succinate repression were cloned in escherichia coli k-12. expression of both wild-type and mutant p. rettgeri acylase genes in e. coli k-12 was independent of orientation in the cloning vehicle and apparently resulted from recognition in e. coli of the p. rettgeri promoter sequences. the p. rettgeri acylase was secreted into the e. coli periplasmic space and was composed of ...	1986	3531181
analysis of thyroglobulin antibody synthesis by cultured peripheral blood lymphocytes from patients with hashimoto's thyroiditis using biotin-avidin solid phase enzyme immuno-assay.	the influence of bovine thyroglobulin (tg) and/or staphylococcus aureus cowan i (sac) on tg antibody synthesis has been studied using cultures of 8 hashimoto's and 5 normal peripheral blood lymphocytes (pbl). the detection of tg antibody in the culture supernatants was performed by sensitive biotin-avidin solid phase enzyme immunoassay. by using this technique, we were able to detect small amounts of tg antibody synthesized by cultured hashimoto's pbl responsive to bovine tg and/or sac; pbl from ...	1986	3539581
alternative protein products with different carboxyl termini from a single class i gene, h-2kb.	in addition to mrna encoding the canonical form of the murine class i antigen h-2kb (348 amino acid residues), mrna that would encode a shortened form of h-2kb (missing 9 amino acids from the c-terminus) has been identified in c57bl/6 spleen cells by rnase-protection studies. the alternative transcripts of h-2kb arise through the use of different ag acceptor splice sites for exon viii. the existence of a shortened h-2kb protein was demonstrated by sequential immunoprecipitation. lysates of splee ...	1986	3461476
[hemoglobin g georgia in a turkish family in the netherlands].		1986	3754310
feline dysautonomia distribution.		1985	4060553
determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection.		1985	3907412
powerline frequency electric and magnetic fields: a pilot study of risk perception.	the perception of the potential risk arising from human exposure to 50/60 hz electric and magnetic fields was studied with a quasi-random sample of 116 well-educated, opinion leaders using the risk perception framework previously developed by slovic, fischhoff, and lichtenstein. these individuals rated exposure to fields from transmission lines and electric blankets on a variety of scales that have been found useful in characterizing people's risk attitudes and perceptions. these judgments allow ...	1985	3843677
aztreonam in the treatment of serious orthopedic infections.	aztreozam was evaluated in the treatment of a variety of orthopedic infections. included were 17 patients with osteomyelitis, three with purulent arthropathy with prostheses, and 16 with superficial infections secondary to trauma or surgical procedure. pathogens were gram-negative bacilli sensitive to aztreonam. concomitant antibiotics were administered for gram-positive cocci that were present initially or by superinfection. infecting organisms included pseudomonas aeruginosa (minimal inhibitor ...	1985	3881949
in vitro studies on the antibacterial activities of ym-13115, a new broad-spectrum cephalosporin.	the in vitro antibacterial activities of ym-13115, a new parenteral cephalosporin, were compared with those of ceftazidime, cefoperazone, and cefsulodin. the compound was highly active against the common members of the enterobacteriaceae and 2 to 256 times more active than cefoperazone. ym-13115 was as active as ceftazidime against citrobacter freundii, proteus vulgaris, and morganella morganii and two to four times more active than ceftazidime against escherichia coli, klebsiella pneumoniae, en ...	1985	3890729
[possible production of 2 cytotoxins by a strain of escherichia coli].		1985	3893462
in vivo and in vitro studies of urinary concentrating ability in potassium-depleted rabbits.	the factors responsible for the urinary concentrating defect associated with the potassium-depleted (kd) state are uncertain. the present studies were designed to, first, determine whether a urinary concentrating defect exists in potassium-depleted rabbits and, second, to use the technique of in vitro perfusion to evaluate directly the antidiuretic hormone (adh) responsiveness of cortical collecting tubules (cct) in this setting. feeding female new zealand white rabbits a potassium-deficient die ...	1985	2993361
report on the conference on aging, reproduction, and the climacteric.		1985	3965314
a comparative examination of the in vitro metabolism of five cyclopenta[a]phenanthrenes of varying carcinogenic potential.	metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. identifications of individual metabolites were based upon elution times, u.v. spectra, and in some cases by mass spectrometry, circular dichroism, and identity with synthetic derivat ...	1985	4017190
a comparative examination of the in vitro metabolism of five cyclopenta[a]phenanthrenes of varying carcinogenic potential.	metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. identifications of individual metabolites were based upon elution times, u.v. spectra, and in some cases by mass spectrometry, circular dichroism, and identity with synthetic derivat ...	1985	4017190
role of protein subunits in proteus rettgeri penicillin g acylase.	penicillin g acylase from proteus rettgeri is an 80,000- to 90,000-dalton enzyme composed of two nonidentical subunits. both subunits were required for enzymatic activity. the 65,000-dalton beta subunit contained a phenylmethylsulfonyl fluoride-sensitive residue required for enzymatic activity, and the 24,500-dalton alpha subunit contained the domain that imparts specificity for the penicillin side chain.	1985	4030697
experimental evolution of penicillin g acylases from escherichia coli and proteus rettgeri.	proteus rettgeri and escherichia coli w were shown to express structurally different penicillin g acylases. the enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. when the organisms were subjected to environmental conditions which made expression of this enzyme essential for growth, spontaneous mutants were isolated that used different amides as the only source o ...	1985	3897200
equivalence of mycobactins from mycobacterium senegalense, mycobacterium farcinogenes and mycobacterium fortuitum.	mycobactins were isolated from five strains designated mycobacterium farcinogenes and a similar number designated mycobacterium senegalense following growth under conditions of iron-limitation. these lipid-soluble iron-chelating compounds were characterized by a combination of thin-layer and high-performance liquid chromatography. the mycobactins from both the slow-growing m. farcinogenes and the rapidly-growing m. senegalense strains proved impossible to differentiate both from each other and f ...	1985	4045424
effect of subinhibitory concentrations of josamycin on the expression of m protein by group a streptococci.	in an attempt to inhibit the biosynthesis of the type-specific m protein usually expressed on surface fimbriae group a streptococcus pyogenes delta 2305 was cultivated in todd-hewitt broth containing 10% human serum and subinhibitory concentrations of either josamycin, erythromycin or clindamycin. electron microscopy revealed that the antibiotic-pretreatment had little visible effect on the surface structures of the streptococci. however, josamycin and clindamycin-pretreated bacteria adhered les ...	1985	3894016
radio-iodine treatment of hyperthyroidism--a more liberal policy?	the main and most valid evidence we have is that on direct follow-up of treated patients. this has been well done in thousands of patients in two countries with very good medical care and over long periods--10 to 15 years or more. on the other hand there is no doubt about radiation carcinogenesis, and thyroid cancer has been caused in children by low-dose x-ray therapy. the possibility of other cancer and of leukaemogenesis is also real. the evidence for this is reviewed and risk estimates given ...	1985	4064351
osteoarthrosis in human cadaveric lumbo-sacral zygapophyseal joints.	this paper reviews some theories expressed in the literature regarding the possible mechanisms involved in osteoarthrosis (degenerative joint disease). preliminary histological evidence of an extensive vascular supply to the osteoarthritic cartilage at the lumbo-sacral zygapophyseal joint in a 56 year old human cadaver is presented.	1985	4078503
cytotoxicity of cortisone-resistant lymphocytes from mice treated with a group a streptococcus or freund's complete adjuvant against tumor cells.	the cortisone-resistant lymphocytes (cr lymphocytes) of mice treated with a group a streptococcus, su strain, or freund's complete adjuvant (fca) were examined for their cytotoxicity on ehrlich carcinoma cells and sarcoma-180 cells. female mice of the ddy strain, 7-8 weeks of age, were injected subcutaneously with streptococci or fca in emulsion, and they were killed 14 days later. to obtain cr lymphocytes, mice treated with and without agents were injected intraperitoneally with hydrocortisone ...	1985	3910898
mitochondrial dna synthesis in non-growth condition (liquid holding) in saccharomyces cerevisiae. relation with growth stages and mitochondrial dna repair after uv-irradiation.		1985	3911223
aztreonam therapy for complicated urinary tract infections caused by multidrug-resistant bacteria.	aztreonam was administered to 145 consecutive patients with complicated urinary tract infections caused by multidrug-resistant, aztreonam-sensitive bacteria. multidrug resistance was defined by disk diffusion testing as resistance to aminopenicillins and to first- and second-generation cephalosporins, with or without resistance to aminoglycosides. the first 40 assessable patients received 1 g of aztreonam intravenously three times daily for a median period of eight days; the remaining 95 assessa ...	1985	3909335
ceftriaxone against gram-negative and gram-positive bacteria: bactericidal and post-antibiotic effect.	the effect of ceftriaxone against gram-negative and gram-positive strains of different clinical isolates has been compared to that of other cephalosporins such as cefuroxime, cefoxitin and cefotaxime. cefotaxime and ceftriaxone have a notably higher activity against enterobacteriaceae than cefuroxime and cefoxitin. if ceftriaxone activity is compared to cefotaxime activity, it may be seen that the former shows a stronger action against pseudomonas, proteus rettgeri, enterobacter and serratia. ag ...	1985	3875430
[cefotiam concentration in exudates from the drainage of patients with acute peritonitis following intravenous administration].	cefotiam dihydrochloride (ctm) in a dose of 2 g was given by intravenous bolus injection to 15 patients operated upon for acute or subacute abdominal organs. they were included to 5 cases of acute localized peritonitis due to perforative appendicitis, 3 of diffuse peritonitis due to perforative duodenal ulcer, 1 of diffuse peritonitis with intestinal obstruction, 1 of perforative sigmoid colon cancer, 2 of gastric cancer or polyp with cholelithiasis, and 3 of cholangitis with cholecyst-choledoch ...	1985	3862880
[antigenic characteristics of standard strains of providencia rettgeri (o- and h-antigens)].	additions and changes have been introduced into the existing antigenic diagnostic scheme of p. rettgeri on the basis of the study of the antigenic structure of standard strains from foreign collections: new, formerly unknown varieties of somatic and flagellar antigens (o35, o36, h27, h28) have been discovered, the complex of antigenic factors for h-antigens 7, 10, 23, 27 has been discovered. strains 958 (36 : 28) and 979 (16 : 27a, 23b, 2a), previously classified with the genus morganella, have ...	1985	2416153
[reactivity to bacterial peptidoglycans in a phagocytosis system. the range of immunological specificity of escherichia coli peptidoglycan].	the antigenic specificity of e. coli m17 peptidoglycans (pg) was studied in the test system based on the stimulation of neutrophils with pg opsonized with the whole and exhausted igg fraction of human serum. within the generic taxon, the pg under study were similar in their antigenic properties. the opsonic activity of igg exhausted with the preparations of different e. coli strains decreased by 93.2-95.8%. an essential difference of e. coli pg from similar components of other representatives of ...	1985	2413663
in vitro study of the sensitivity of 1030 hospital-isolated bacterial strains to aztreonam.	the antibacterial activity in vitro of aztreonam is compared with other antibiotics on 1,030 strains isolated from pathological specimens. its spectrum is very large, with special regard to gram-negative bacteria. of particular interest appears the sensitivity to aztreonam of otherwise multiresistant strains of pseudomonas aeruginosa and proteus rettgeri.	1984	6543433