Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
development of a prediction model for bacteremia in hospitalized adults with cellulitis to aid in the efficient use of blood cultures: a retrospective cohort study.cellulitis is a common infectious disease. although blood culture is frequently used in the diagnosis and subsequent treatment of cellulitis, it is a contentious diagnostic test. to help clinicians determine which patients should undergo blood culture for the management of cellulitis, a diagnostic scoring system referred to as the bacteremia score of cellulitis was developed.201627756213
multi-omics analysis on the pathogenicity of enterobacter cloacae enhku01 isolated from sewage outfalls along the ningbo coastline.the acquisition of iron is important for the pathogenicity of bacteria and blood. three different culture environments (fe stimulation, blood agar plate and normal plate) were used to stimulate enterobacter cloacae, and their respective pathogenicities were compared at the proteomic, mrna and metabolomic levels.201627777513
aquatic plant azolla as the universal feedstock for biofuel production.the quest for sustainable production of renewable and cheap biofuels has triggered an intensive search for domestication of the next generation of bioenergy crops. aquatic plants which can rapidly colonize wetlands are attracting attention because of their ability to grow in wastewaters and produce large amounts of biomass. representatives of azolla species are some of the fastest growing plants, producing substantial biomass when growing in contaminated water and natural ecosystems. together wi ...201627777623
reduced susceptibility to extended-spectrum β-lactams in vibrio cholerae isolated in bangladesh.β-lactams are antibiotic molecules able to inhibit cell wall biosynthesis. among other mechanisms, resistance in gram-negative bacteria is mostly associated with production of β-lactamase enzymes able to bind and hydrolyze the β-lactam ring. extended-spectrum β-lactamases extend this ability also to third- and fourth-generation cephalosporins, as well as to carbapenems and monobactams. vibrio cholerae is the causative agent of epidemic cholera and a public health burden for developing countries ...201627803895
application and uses of electronic noses for clinical diagnosis on urine samples: a review.the electronic nose is able to provide useful information through the analysis of the volatile organic compounds in body fluids, such as exhaled breath, urine and blood. this paper focuses on the review of electronic nose studies and applications in the specific field of medical diagnostics based on the analysis of the gaseous headspace of human urine, in order to provide a broad overview of the state of the art and thus enhance future developments in this field. the research in this field is ra ...201627754437
antibacterial activity of six medicinal cameroonian plants against gram-positive and gram-negative multidrug resistant phenotypes.infectious diseases due to multidrug-resistant bacteria are one of the causes of treatment failures contributing to an increase in mortality and/or morbidity. in this study, we evaluated the antibacterial potential of different parts of six medicinal plants namely alstonia boonei, ageratum conyzoides, croton macrostachys, cassia obtusifolia, catharanthus roseus and paullinia pinnata against a panel of 36 multi-drug resistant (mdr) gram-negative and gram-positive bacteria.201627724917
impact of hiv infection on the presentation, outcome and host response in patients admitted to the intensive care unit with sepsis; a case control study.sepsis is a prominent reason for intensive care unit (icu) admission in patients with hiv. we aimed to investigate the impact of hiv infection on presentation, outcome and host response in sepsis.201627719675
a novel fe(iii) dependent bioflocculant from klebsiella oxytoca gs-4-08: culture conditions optimization and flocculation mechanism.in this work, the effect of cultivation factors on the flocculation efficiency (fe) of bioflocculant p-gs408 from klebsiella oxytoca was optimized by the response surface methodology. the most significant factor, i.e. culture time, was determined by gray relational analysis. a total of 240 mg of purified p-gs408 was prepared from 1 liter of culture solution under the optimal conditions. gc-ms analysis results indicated that the polysaccharide of p-gs408 mainly contains rhamnose and galactose, an ...201627713559
carbapenem-resistant klebsiella pneumoniae influences the outcome of early infections in liver transplant recipients.infections remain a leading cause of morbidity and mortality among liver transplant (lt) recipients. the aim of our study was to define the factors associated with outcome of early bacterial and fungal infections in a cohort of patients who underwent lt at the university hospital of ancona over a nine year period.201627716164
expression, purification and immobilization of tannase from staphylococcus lugdunensis mtcc 3614.enzymes find their applications in various industries, due to their error free conversion of substrate into product. tannase is an enzyme used by various industries for degradation of tannin. biochemical characterization of a specific enzyme from one organism to other is one of the ways to search for enzymes with better traits for industrial applications. here, tannase encoding gene from staphylococcus lugdunensis was cloned and suitability of the enzyme in various conditions was analysed to fin ...201627704471
pitfalls in interpretation of fdg pet/ct: septic pulmonary emboli mimicking metastases in a case of gastric carcinoma.inflammatory lesions may sometimes show intense tracer uptake and mimic neoplastic lesions on (18) f-fluoro-deoxyglucose (fdg) positron emission tomography/computed tomography (pet/ct). we report one such false positive case on fdg pet/ct, where septic pulmonary emboli (spe) mimicked pulmonary metastases. a 45-year-old man with stomach cancer had an indwelling central venous catheter (cvc) in situ while on neoadjuvant chemotherapy. he underwent fdg pet/ct scan for response assessment and the ima ...201628104952
the efficacy of noble metal alloy urinary catheters in reducing catheter-associated urinary tract infection.catheter-associated urinary tract infection (cauti) is the most common device-related healthcare-acquired infection. cauti can be severe and lead to bacteremia, significant morbidity, prolonged hospital stay, and high antibiotic consumption.201628057985
emphysematous pyelonephritis: the impact of urolithiasis on disease severity.emphysematous pyelonephritis is a severe infection of the kidney associated with formation of gas in the renal parenchyma and/or collecting system. the purpose of this study was to evaluate outcomes of patients with emphysematous pyelonephritis in a contemporary cohort and to evaluate the impact of urolithiasis on disease severity.201627785435
hyperbaric oxygen therapy as additional treatment in deep sternal wound infections - a single center's experience.deep sternal wound infection (dswi) is one of the most serious complications after cardiac surgery procedures, observed in 5% of patients. current standard medical therapy for dswi includes antibiotics, surgical debridement, resuturing or negative pressure wound therapy (npwt). unfortunately, in some cases these methods are insufficient, and additional therapeutic options are needed.201627785131
current state of resistance to antibiotics of last-resort in south africa: a review from a public health perspective.a review of the literature was undertaken to delineate the current level and mechanisms of resistance to carbapenems, colistin, and tigecycline in south africa. thirty-two english publications and 32 national institute of communicable diseases communiqués identified between early january 2000 and 20 may, 2016 showed substantial reports of ndm (n = 860), oxa-48 (n = 584), vim (n = 131), and imp (n = 45) carbapenemases within this period, mainly in klebsiella pneumoniae (n = 1138), acinetobacter b ...201627747206
pharmacokinetics of gentamicin eluted from a regenerating bone graft substitute: in vitro and clinical release studies.deep bone and joint infections (dbji) are directly intertwined with health, demographic change towards an elderly population, and wellbeing.the elderly human population is more prone to acquire infections, and the consequences such as pain, reduced quality of life, morbidity, absence from work and premature retirement due to disability place significant burdens on already strained healthcare systems and societal budgets.dbjis are less responsive to systemic antibiotics because of poor vascular p ...201627678329
role of nf-kβ factor rel2 during plasmodium falciparum and bacterial infection in anopheles dirus.anopheles mosquitoes transmit malaria which is one of the world's most threatening diseases. anopheles dirus (sensu stricto) is among the main vectors of malaria in south east asia. the mosquito innate immune response is the first line of defence against malaria parasites during its development. the immune deficiency (imd) pathway, a conserved immune signaling pathway, influences anti-plasmodium falciparum activity in anopheles gambiae, an. stephensi and an. albimanus. the aim of the study was t ...201627688040
are community-based hospitals safe from carbapenem-resistant enterobacteriaceae in korea? 201627704734
open surgical versus minimal invasive necrosectomy of the pancreas-a retrospective multicenter analysis of the german pancreatitis study group.necrotising pancreatitis, and particularly infected necrosis, are still associated with high morbidity and mortality. since 2011, a step-up approach with lower morbidity rates compared to initial open necrosectomy has been established. however, mortality and complication rates of this complex treatment are hardly studied thereafter.201627668746
extended-spectrum ß-lactamase-producing enterobacteriaceae as a common cause of urinary tract infections in sri lanka.extended-spectrum ß-lactamase-producing enterobacteriaceae (esbl-pe) are increasingly reported as pathogens in urinary tract infections (utis). however, in sri lanka, the clinical and molecular epidemiology of esbl-pe implicated in utis has not been well described.201627704730
interaction of avibactam with class b metallo-β-lactamases.β-lactamases are the most important mechanisms of resistance to the β-lactam antibacterials. there are two mechanistic classes of β-lactamases: the serine β-lactamases (sbls) and the zinc-dependent metallo-β-lactamases (mbls). avibactam, the first clinically useful non-β-lactam β-lactamase inhibitor, is a broad-spectrum sbl inhibitor, which is used in combination with a cephalosporin antibiotic (ceftazidime). there are multiple reports on the interaction of avibactam with sbls but few such studi ...201627401561
macrolide resistance in streptococcus pneumoniae.streptococcus pneumoniae is a common commensal and an opportunistic pathogen. suspected pneumococcal upper respiratory infections and pneumonia are often treated with macrolide antibiotics. macrolides are bacteriostatic antibiotics and inhibit protein synthesis by binding to the 50s ribosomal subunit. the widespread use of macrolides is associated with increased macrolide resistance in s. pneumoniae, and the treatment of pneumococcal infections with macrolides may be associated with clinical fai ...201627709102
cathelicidin antimicrobial peptides with reduced activation of toll-like receptor signaling have potent bactericidal activity against colistin-resistant bacteria.the world is at the precipice of a postantibiotic era in which medical procedures and minor injuries can result in bacterial infections that are no longer effectively treated by antibiotics. cathelicidins are peptides produced by animals to combat bacterial infections and to regulate innate immune responses. however, cathelicidins are potent activators of the inflammatory response. cathelicidins with reduced proinflammatory activity and potent bactericidal activity in the low micromolar range ag ...201627651360
the role of dna amplification and cultural growth in complicated acute appendicitis.bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. dna amplification represents a fast technique to detect microbial sequences. we aimed to compare the potential of dna amplification versus traditional bacterial growth culture highlighting advantages and drawbacks in a surgical setting. peritoneal fluid specimens were collected during surgery from 36 children who unde ...201627777701
role of gut microbiota in the aetiology of obesity: proposed mechanisms and review of the literature.the aetiology of obesity has been attributed to several factors (environmental, dietary, lifestyle, host, and genetic factors); however none of these fully explain the increase in the prevalence of obesity worldwide. gut microbiota located at the interface of host and environment in the gut are a new area of research being explored to explain the excess accumulation of energy in obese individuals and may be a potential target for therapeutic manipulation to reduce host energy storage. several me ...201627703805
therapeutic drug monitoring of meropenem in neonate with necrotizing enterocolitis: a challenge.necrotizing enterocolitis (nec) continues to be a major cause of neonatal morbidity and mortality. we describe the added value of therapeutic drug monitoring by presenting the case of a preterm infant with severe nec treated with meropenem. dosing strategy will achieve adequate patient outcome when treating pathogens with elevated mic. as safe as meropenem is, there are not enough data for 40 mg/kg, every 8 h infused over 4 h; accordingly, strict monitoring of blood levels is mandatory. based on ...201627703820
national survey of colistin resistance among carbapenemase-producing enterobacteriaceae and outbreak caused by colistin-resistant oxa-48-producing klebsiella pneumoniae, france, 2014.from january 2014 to december 2014, 972 consecutive non-replicate carbapenemase-producing enterobacteriaceae isolates from colonised or infected patients were collected at the associated french national reference centre as part of the french national survey on antimicrobial resistance. it included 577 klebsiella spp. (59%), 236 escherichia coli (24%), 108 enterobacter spp. (11%), 50 citrobacter spp. (5%), and a single salmonella spp. isolate (0.1%). of 561 k. pneumoniae isolates, 35 were found t ...201627685838
zinc pyrithione improves the antibacterial activity of silver sulfadiazine ointment.pseudomonas aeruginosa, acinetobacter baumannii, and staphylococcus aureus are commonly associated with biofilm-associated wound infections that are recalcitrant to conventional antibiotics. as an initial means to identify agents that may have a greater propensity to improve clearance of wound-associated bacterial pathogens, we screened a food and drug administration-approved drug library for members that display bactericidal activity toward 72-h-established p. aeruginosa biofilms using an adeny ...201627642637
type 3 fimbriae encoded on plasmids are expressed from a unique promoter without affecting host motility, facilitating an exceptional phenotype that enhances conjugal plasmid transfer.horizontal gene transfer (hgt), the transmission of genetic material to a recipient that is not the progeny of the donor, is fundamental in bacterial evolution. hgt is often mediated by mobile genetic elements such as conjugative plasmids, which may be in conflict with the chromosomal elements of the genome because they are independent replicons that may petition their own evolutionary strategy. here we study differences between type 3 fimbriae encoded on wild type plasmids and in chromosomes. u ...201627627107
multidisciplinary approach to giant paratesticular liposarcoma.primary paratesticular tumours are very rare and the spermatic cord (sct) is the most frequent site of origin, with 20% of malignancy. although liposarcoma is the most frequent histotype (46.6 %), less than 200 cases have been reported in the literature. we report the case of a 56-year-old man who presented a giant scrotal mass of 25 years of evolution and measuring 40 × 40 cm. it could be considered the greatest paratesticular liposarcoma described to date. computed tomogaphy (ct) revealed mass ...201627695588
maldi-tof ms versus vitek(®)2: comparison of systems for the identification of microorganisms responsible for bacteremia.we evaluated the reliability and accuracy of the combined use of maldi-tof ms and classical id vitek2 to identify monomicrobial infection in blood culture bottles. in total, 70 consecutive positive blood cultures were included in this study. positive blood culture bottles were subjected to gram staining and subcultured on solid media. isolates grown from such culture media were used for classical id using vitek2 system. in parallel, an aliquot was subjected to a lysing-centrifugation method and ...201627620383
occurence of arma and rmtb aminoglycoside resistance 16s rrna methylases in extended-spectrum β-lactamases producing escherichia coli in algerian hospitals.the aim of this study, was to characterize the extended-spectrum-β-lactamases (esbls) producing clinical strains of escherichia coli isolated between january 2009 and june 2012 from algerian hospitals and to determine the prevalence of 16s rrna methylase among them. sixty-seven esbl-producers were detected among the 239 isolates included: 52 ctx-m-15-producers, 5 ctx-m-3-producers, 5 ctx-m-1-producers, 2 ctx-m-14-producers, 2 shv-12-producers and one tem-167-producer. among the esbl-producing st ...201627672380
munchausen syndrome disguised as gossypiboma: an interesting case. 201627651853
in vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments.there is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. biofilms consist of polymicrobial communities that survive the host's defense system. the key bacteria in biofilms are slow growing and support an attachment and rapid growth of other microorganisms. current antimicrobial strategies often fail due to poor diagnosis of key pathogens in biofilms. the study aims to develop anti-bacterial human antibodies in vitro fro ...201627612600
process optimization on micro-aeration supply for high production yield of 2,3-butanediol from maltodextrin by metabolically-engineered klebsiella oxytoca.an optimization process with a cheap and abundant substrate is considered one of the factors affecting the price of the production of economical 2,3-butanediol (2,3-bd). a combination of the conventional method and response surface methodology (rsm) was applied in this study. the optimized levels of ph, aeration rate, agitation speed, and substrate concentration (maltodextrin) were investigated to determine the cost-effectiveness of fermentative 2,3-bd production by metabolically-engineered kleb ...201627603922
enhancement of calibrachoa growth, secondary metabolites and bioactivity using seaweed extracts.calibrachoa x hybrida (solanaceae) cultivars are widely used in north and south america as ornamental plants. their potential as a source of antimicrobial compounds might be enhanced by seaweed extract (swe) applications.201627589868
infectious pseudomonas and bipolaris scleritis following history of pterygium surgery.we report an interesting case of infectious scleritis from coinfection of pseudomonas aeruginosa and bipolaris with no corneal infiltrate. a healthy 60-year-old man with a history of infectious scleritis following pterygium excision presented with purulent material growing p. aeruginosa and 1+ colonies of bipolaris species of fungus. broad spectrum treatment was initiated with hourly topical moxifloxacin, fortified tobramycin, and natamycin along with a subconjunctival injection of voriconazole ...201627853018
resistance mechanisms.by definition, the terms sepsis and septic shock refer to a potentially fatal infectious state in which the early administration of an effective antibiotic is the most significant determinant of the outcome. because of the global spread of resistant bacteria, the efficacy of antibiotics has been severely compromised. s. pneumonia, escherichia coli (e. coli), klebsiella, acinetobacter, and pseudomonas are the predominant pathogens of sepsis and septic shock. it is common for e. coli, klebsiella, ...201627713884
molecular epidemiology of carbapenem resistant gram-negative bacilli from infected pediatric population in tertiary - care hospitals in medellín, colombia: an increasing problem.gram-negative bacilli are a cause of serious infections in the pediatric population. carbapenem are the treatment of choice for infections caused by multidrug-resistant gram-negative bacilli, but the emergence of carbapenem resistance has substantially reduced access to effective antimicrobial regimens. children are a population vulnerable to bacterial infections and the emergence of resistance can worsen prognosis. the aim of this study is to describe the clinical and molecular characteristics ...201627585707
colistin-resistant enterobacteriaceae carrying the mcr-1 gene among patients in hong kong. 201627532341
carbapenem-resistant enterobacter spp. in retail seafood imported from southeast asia to canada. 201627533255
in silico analysis of glutaminase from different species of escherichia and bacillus.glutaminase (ec 3.5.1.2) catalyzes the hydrolytic degradation of l-glutamine to l-glutamic acid and has been introduced for cancer therapy in recent years. the present study was an in silico analysis of glutaminase to further elucidate its structure and physicochemical properties.201627582590
development of 11-plex mol-pcr assay for the rapid screening of samples for shiga toxin-producing escherichia coli.strains of shiga toxin-producing escherichia coli (stec) are a serious threat to the health, with approximately half of the stec related food-borne illnesses attributable to contaminated beef. we developed an assay that was able to screen samples for several important stec associated serogroups (o26, o45, o103, o104, o111, o121, o145, o157) and three major virulence factors (eae, stx 1 , stx 2) in a rapid and multiplexed format using the multiplex oligonucleotide ligation-pcr (mol-pcr) assay che ...201627630828
aminoglycoside antibiotics for nih category ii chronic bacterial prostatitis: a single-cohort study with one-year follow-up.although fluoroquinolones are first-line agents for the treatment of national institutes of health (nih) category ii chronic bacterial prostatitis (cbp), therapy with these agents is not always feasible due to the increasing worldwide resistance of causative uropathogens. new therapeutic options are urgently required, as drugs such as β-lactam antibiotics distribute poorly to prostatic sites of infection and trimethoprim therapy is often unfeasible due to high resistance rates. the present study ...201627698761
the membrane steps of bacterial cell wall synthesis as antibiotic targets.peptidoglycan is the major component of the cell envelope of virtually all bacteria. it has structural roles and acts as a selective sieve for molecules from the outer environment. peptidoglycan synthesis is therefore one of the most important biogenesis pathways in bacteria and has been studied extensively over the last twenty years. the pathway starts in the cytoplasm, continues in the cytoplasmic membrane and finishes in the periplasmic space, where the precursor is polymerized into the pepti ...201627571111
scarcity of autoreactive human blood iga(+) memory b cells.class-switched memory b cells are key components of the "reactive" humoral immunity, which ensures a fast and massive secretion of high-affinity antigen-specific antibodies upon antigenic challenge. in humans, iga class-switched (iga(+) ) memory b cells and iga antibodies are abundant in the blood. although circulating iga(+) memory b cells and their corresponding secreted immunoglobulins likely possess major protective and/or regulatory immune roles, little is known about their specificity and ...201627469325
genome sequence of enterobacter cloacae strain seng-6, a bacterium producing histo-blood group antigen-like substances that can bind with human noroviruses.enterobacter sp. strain seng-6, isolated from healthy human feces, produces histo-blood group antigen (hbga)-like substances that can bind with human noroviruses. based on the genome sequence analysis, strain seng-6 belongs to the species enterobacter cloacae the genome sequence of this strain should help identify genes associated with the production of hbga-like substances.201627563051
a novel integron gene cassette harboring vim-38 metallo-β-lactamase in a clinical pseudomonas aeruginosa isolate. 201627578518
draft genome sequence of endophytic bacterium enterobacter asburiae pda134, isolated from date palm (phoenix dactylifera l.) roots.in this report, a draft of the enterobacter asburiae strain pda134 genome was sequenced. this bacterial strain was isolated from the root tissue of a date palm, where it has the ability to produce 1-aminocyclopropane-1-carboxylic acid (acc) deaminase and indole-3-acetic acid (iaa) under salinity stress.201627540071
inactivation of cytomegalovirus in breast milk using ultraviolet-c irradiation: opportunities for a new treatment option in breast milk banking.pasteurized donor human milk is provided by milk banks to very preterm babies where their maternal supply is insufficient or unavailable. donor milk is currently processed by holder pasteurization, producing a microbiologically safe product but significantly reducing immunoprotective components. ultraviolet-c (uv-c) irradiation at 254 nm is being investigated as an alternative treatment method and has been shown to preserve components such as lactoferrin, lysozyme and secretory iga considerably ...201627537346
microbiological characteristics and predictive factors for mortality in pleural infection: a single-center cohort study in korea.identification and understanding of the pathogens responsible for pleural infection is critical for appropriate antibiotic treatment. this study sought to determine the microbiological characteristics of pleural infection and to identify potential predictive factors associated with mortality.201627529628
evaluation of staphylococcus aureus eradication therapy in vascular surgery.surgical site infections (ssi) are a serious complication in vascular surgery which may lead to severe morbidity and mortality. staphylococcus aureus nasal carriage is associated with increased risk for development of ssis in central vascular surgery. the risk for ssi can be reduced by perioperative eradication of s. aureus carriage in cardiothoracic and orthopedic surgery. this study analyzes the relation between s. aureus eradication therapy and ssi in a vascular surgery population.201627529551
technological factors affecting biogenic amine content in foods: a review.biogenic amines (bas) are molecules, which can be present in foods and, due to their toxicity, can cause adverse effects on the consumers. bas are generally produced by microbial decarboxylation of amino acids in food products. the most significant bas occurring in foods are histamine, tyramine, putrescine, cadaverine, tryptamine, 2-phenylethylamine, spermine, spermidine, and agmatine. the importance of preventing the excessive accumulation of bas in foods is related to their impact on human hea ...201627570519
a new rapid method for direct antimicrobial susceptibility testing of bacteria from positive blood cultures.rapid identification and antimicrobial susceptibility testing (ast) of the causative agent(s) of bloodstream infections can lead to prompt appropriate antimicrobial therapy. to shorten species identification, in this study bacteria were recovered from monomicrobial blood cultures by serum separator tubes and spotted onto the target plate for direct maldi-tof ms identification. proper antibiotics were selected for direct ast based on species identification. in order to obtain rapid ast results, b ...201627520338
the etiology, incidence, and impact of preservation fluid contamination during liver transplantation.the role of contaminated preservation fluid in the development of infection after liver transplantation has not been fully elucidated. to assess the incidence and etiology of contaminated preservation fluid and determine its impact on the subsequent development of infection after liver transplantation, we prospectively studied 50 consecutive liver transplants, and cultured the following samples in each instance: preservation fluid (immediately before and at the end of the back-table procedure, a ...201627513941
a flow cytometric and computational approaches to carbapenems affinity to the different types of carbapenemases.the synergy of carbapenem combinations regarding enterobacteriaceae producing different types of carbapenemases was study through different approaches: flow cytometry and computational analysis. ten well characterized enterobacteriaceae (kpc, verona integron-encoded metallo-β-lactamases -vim and oxa-48-like enzymes) were selected for the study. the cells were incubated with a combination of ertapenem with imipenem, meropenem, or doripenem and killing kinetic curves performed with and without rei ...201627555844
prospective evaluation of septifast multiplex pcr in children with systemic inflammatory response syndrome under antibiotic treatment.antimicrobially pre-treated children with systemic inflammation often pose a diagnostic challenge to the physician. we aimed to evaluate the additional use of septifast multiplex polymerase chain reaction (pcr) to identify causative pathogens in children with suspected systemic bacterial or fungal infection.201627503068
late-onset deep mesh infection: a study of eight cases detected from 2666 consecutive patients with abdominal wall hernia repairs. 201627453240
tigecycline-based versus sulbactam-based treatment for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex.the treatment options for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii (mdr acb) complex are limited, and the optimal treatment has not been established.201627496018
comparison of methods for the identification of microorganisms isolated from blood cultures.bloodstream infections are responsible for thousands of deaths each year. the rapid identification of the microorganisms causing these infections permits correct therapeutic management that will improve the prognosis of the patient. in an attempt to reduce the time spent on this step, microorganism identification devices have been developed, including the vitek(®) 2 system, which is currently used in routine clinical microbiology laboratories.201627496125
a locking compression plate as an external fixator for treating infected nonunion of the humeral diaphysis.infected nonunion of the humeral diaphysis is a challenging problem for orthopedic surgeons. this study aimed to evaluate the outcome of using a locking compression plate (lcp) as a definitive external fixator in the management of infected nonunion of the humeral diaphysis after failure of internal fixation.201627491400
hydroxylation of diverse flavonoids by cyp450 bm3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities.cytochrome p450 monooxygenase constitutes a significant group of oxidative enzymes that can introduce an oxygen atom in a high regio- and stereo-selectivity mode. we used the bacillus megaterium cytochrome p450 bm3 (cyp450 bm3) and its variants namely mutant 13 (m13) and mutant 15 (m15) for the hydroxylation of diverse class of flavonoids.201627495155
fructose 1,6-bisphosphate aldolase, a novel immunogenic surface protein on listeria species.listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. increased understanding of the surface exposed antigens on listeria would provide potential diagnostic and therapeutic targets. in the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (fba) class ii in listeria species is the antigen target of the previously described mab-3f8. western and ...201627489951
outbreak by ventilator-associated st11 k. pneumoniae with co-production of ctx-m-24 and kpc-2 in a sicu of a tertiary teaching hospital in central china.the emergence of carbapenem-resistant klebsiella pneumoniae (crkp) often responsible for numerous hospital-associated outbreaks has become an important public health problem. from january 2013 to february 2014, a total of 41 non-duplicate k. pneumoniae isolates with carbapenem resistance, were collected at a tertiary teaching hospital in nanchang, central china. among 41 k. pneumoniae isolates, 28 were isolated from hospitalized patients including 19 from the patients in surgery intensive care u ...201627531996
community-onset bloodstream and other infections, caused by carbapenemase-producing enterobacteriaceae: epidemiological, microbiological, and clinical features.background.  because most infections caused by carbapenemase-producing enterobacteriaceae (cpe) begin during hospitalization, there are limited data about community-onset (co) infections caused by cpe. our aim is to describe the frequency of co infections caused by cpe as well as the clinical features of co bloodstream infections (co-bsis). methods.  this study includes retrospective case series of co infections caused by cpe in a tertiary hospital from january 2010 to july 2014. any clinical sa ...201627703997
randomized controlled trial evaluating dialkylcarbamoyl chloride impregnated dressings for the prevention of surgical site infections in adult women undergoing cesarean section.surgical site infections (ssi) occur in 1.8%-9.2% of women undergoing cesarean section (cs) and lead to greater morbidity rates and increased treatment costs. the aim of the study was to evaluate the efficacy and cost-effectiveness of dialkylcarbamoyl chloride (dacc) impregnated dressings to prevent ssi in women subject to cs.201626891115
clinical relevance of pathogens detected by multiplex pcr in blood of very-low-birth weight infants with suspected sepsis - multicentre study of the german neonatal network.in the german neonatal network (gnn) 10% of very-low-birth weight infants (vlbwi) suffer from blood-culture confirmed sepsis, while 30% of vlbwi develop clinical sepsis. diagnosis of sepsis is a difficult task leading to potential over-treatment with antibiotics. this study aims to investigate whether the results of blood multiplex-pcr (septifast®) for common sepsis pathogens are relevant for clinical decision making when sepsis is suspected in vlbwi.201627472282
optimization of growth conditions for purification and production of l-asparaginase by spirulina maxima.l-asparaginase (l-asna) is widely distributed among microorganisms and has important applications in medicine and in food technology sectors. therefore, the ability of the production, purification, and characterization of asna from spirulina maxima (sm) were tested. sm cultures grown in zarrouk medium containing different n2 (in nano3 form) concentrations (1.25, 2.50, and 5.0 g/l) for 18 days contained a significant various quantity of dry biomass yields and asna enzyme levels. ms l-asna activit ...201627525017
travelers can import colistin-resistant enterobacteriaceae, including those possessing the plasmid-mediated mcr-1 gene.stool samples from 38 travelers returning from india were screened for extended-spectrum cephalosporin- and carbapenem-resistant enterobacteriaceae implementing standard selective plates. twenty-six (76.3%) people were colonized with ctx-m or dha producers, but none of the strains was colistin resistant and/or mcr-1 positive. nevertheless, using overnight enrichment and chromagar orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistant ...201627297483
erratum for norgan et al., carbapenem- and colistin-resistant enterobacter cloacae from delta, colorado, in 2015. 201627451439
mobilization of blabkc-1 by iskpn23? 201627451437
spread of plasmids carrying multiple ges variants.five ges-producing enterobacteriaceae isolates that displayed an extended-spectrum β-lactamase (esbl) phenotype harbored two ges variants: ges-7 esbl and ges-6 carbapenemase. in all isolates, the two ges alleles were located on the same integron that was inserted into an 80-kb incm1 self-conjugative plasmid. whole-genome sequencing suggested in vivo horizontal gene transfer of the plasmid along with clonal diffusion of enterobacter cloacae to our knowledge, this is the first description in europ ...201627216071
horizontal transfer of carbapenemase-encoding plasmids and comparison with hospital epidemiology data.carbapenemase-producing organisms have spread worldwide, and infections with these bacteria cause significant morbidity. horizontal transfer of plasmids carrying genes that encode carbapenemases plays an important role in the spread of multidrug-resistant gram-negative bacteria. here we investigate parameters regulating conjugation using an escherichia coli laboratory strain that lacks plasmids or restriction enzyme modification systems as a recipient and also using patient isolates as donors an ...201627270289
in vitro emergence of high persistence upon periodic aminoglycoside challenge in the eskape pathogens.health care-associated infections present a major threat to modern medical care. six worrisome nosocomial pathogens-enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter spp.-are collectively referred to as the "eskape bugs." they are notorious for extensive multidrug resistance, yet persistence, or the phenotypic tolerance displayed by a variant subpopulation, remains underappreciated in these pathogens. importantly ...201627185802
biliary microbiota, gallstone disease and infection with opisthorchis felineus.there is increasing interest in the microbiome of the hepatobiliary system. this study investigated the influence of infection with the fish-borne liver fluke, opisthorchis felineus on the biliary microbiome of residents of the tomsk region of western siberia.201627447938
antimicrobial effects of silver nanoparticles stabilized in solution by sodium alginate.to investigate the effect of nanosilver particles in solution stabilized in a matrix of sodium alginate on the growth and development of pathogenic bacteria such as staphylococcus aureus, enterococcus faecalis, escherichia coli, proteus vulgaris, enterobacter cloacae, the antibiotic-resistant strain of pseudomonas aeruginosa, the yeast-like fungus candida albicans, and the luminescent bacteria photobacterium leiognathi sh1.201627747311
fortified interpenetrating polymers - bacteria resistant coatings for medical devices.infections arising from contaminated medical devices are a serious global issue, contributing to antibiotic resistance and imposing significant strain on healthcare systems. since the majority of medical device-associated infections are biofilm related, efforts are being made to generate either bacteria-repellent or antibacterial coatings aimed at preventing bacterial colonisation. here, we utilise a nanocapsule mediated slow release of a natural antimicrobial to improve the performance of a bac ...201627746915
high prevalence of plasmid-mediated quinolone resistance and incq plasmids carrying qnrs2 gene in bacteria from rivers near hospitals and aquaculture in china.effluents from hospital and aquaculture are considered important sources of quinolone resistance. however, little information is available on the impact of this effluent on nearby rivers. in this study, 188 ciprofloxacin-resistant bacterial isolates obtained from rivers near hospitals and aquaculture were screened for plasmid-mediated quinolone resistance (pmqr) genes. species identification, antibiotic susceptibility testing, and pmqr gene transferability assessment were conducted for pmqr-posi ...201627427763
the role of myeloid differentiation factor 88 on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.to investigate the role of myeloid differentiation factor 88 (myd88) on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.201627536200
selective intestinal decontamination for the prevention of early bacterial infections after liver transplantation.bacterial infection in the first month after liver transplantation is a frequent complication that poses a serious risk for liver transplant recipients as contributes substantially to increased length of hospitalization and hospital costs being a leading cause of death in this period. most of these infections are caused by gram-negative bacilli, although gram-positive infections, especially enterococcus sp. constitute an emerging infectious problem. this high rate of early postoperative infectio ...201627468189
physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells.this research aims to study the production of chitosan from shrimp shell (litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. the evaluation of the quality of chitosan from waste shrimp shells includes parameters for the yield, physical chemistry characteristics by infrared spectroscopy (ft-ir), the degree of deacetylation, and antibacterial activity. the results showed (by method 1) extraction yiel ...201627478443
preventing data ambiguity in infectious diseases with four-dimensional and personalized evaluations.diagnostic errors can occur, in infectious diseases, when anti-microbial immune responses involve several temporal scales. when responses span from nanosecond to week and larger temporal scales, any pre-selected temporal scale is likely to miss some (faster or slower) responses. hoping to prevent diagnostic errors, a pilot study was conducted to evaluate a four-dimensional (4d) method that captures the complexity and dynamics of infectious diseases.201627411058
identification of an ndm-5-producing escherichia coli sequence type 167 in a neonatal patient in china.emergence of new delhi metallo-β-lactamase-producing enterobacteriaceae has become a challenging threat to public health. two carbapenem-resistant escherichia coli, strain qd28 and qd29, were recovered from the aspirating sputum of a neonate and the urine of an adult in a chinese hospital in 2013. molecular typing revealed that both isolates belonged to the sequence type 167, but they were clonally diverse. both isolates exhibited resistance to carbapenems, cephalosporins, ciprofloxacin, gentami ...201627406405
outbreak of extended-spectrum beta-lactamase producing enterobacter cloacae with high mics of quaternary ammonium compounds in a hematology ward associated with contaminated sinks.to investigate an outbreak of extended-spectrum beta-lactamase (esbl) producing enterobacter cloacae that occurred in the hematology ward (24-bed unit) of the françois mitterrand university hospital (dijon, france) between january 2011 and december 2013. the outbreak involved 43 patients (10 infected and 33 colonized).201627462306
bartonella henselae as a putative cause of congenital cholestasis.severe anemia and cholestatic hepatitis are associated with bartonella infections. a putative vertical bartonella henselae infection was defined on the basis of ultrastructural and molecular analyses in a three-year-old child with anemia, jaundice and hepatosplenomegaly since birth. physicians should consider bartonellosis in patients with anemia and hepatitis of unknown origin.201627410916
bioactivity of polyphenols: preventive and adjuvant strategies toward reducing inflammatory bowel diseases-promises, perspectives, and pitfalls.inflammatory bowel diseases (ibds) are characterized by autoimmune and inflammation-related complications of the large intestine (ulcerative colitis) and additional parts of the digestive tract (crohn's disease). complications include pain, diarrhoea, chronic inflammation, and cancer. ibd prevalence has increased during the past decades, especially in westernized countries, being as high as 1%. as prognosis is poor and medication often ineffective or causing side effects, additional preventive/a ...201627478535
strategies for prevention of ultrasound-guided prostate biopsy infections.prostate cancer is the most common cancer in male patients and the second leading cause of cancer-related mortality in males. to confirm the diagnosis of prostate cancer, an ultrasound-guided needle biopsy is necessary to obtain prostate tissue sufficient for histologic analysis by pathologists. ultrasound-guided prostate needle biopsy can be accomplished via a transperineal or transrectal approach. the latter biopsy technique involves placing an ultrasound probe into the rectum, visualizing the ...201627468242
failure of the first step of two-stage revision due to polymicrobial prosthetic joint infection of the hip.the unsuccessful treatment of prosthetic joint infection (pji) with two-stage revision leads to infection recurrence. the objectives of the study were to assess the clinical and demographic characteristics of patients with polymicrobial pji, and to evaluate the role of the microbial profile involved in pji in the risk of infection recurrence after the first step of two-stage revision surgery.201627387172
antibacterial and antibiotic-resistance modifying activity of the extracts and compounds from nauclea pobeguinii against gram-negative multi-drug resistant phenotypes.multi-drug resistance of gram-negative bacteria constitutes a major obstacle in the antibacterial fight worldwide. the discovery of new and effective antimicrobials and/or resistance modulators is necessary to combat the spread of resistance or to reverse the multi-drug resistance. in this study, we investigated the antibacterial and antibiotic-resistance modifying activities against 29 gram-negative bacteria including multi-drug resistant (mdr) phenotypes of the methanol extracts from nauclea p ...201627386848
comparison of early onset sepsis and community-acquired late onset sepsis in infants less than 3 months of age.we compared demographic and clinical characteristics of early-onset sepsis (eos) and community-acquired late onset sepsis (ca-los) in infants.201627387449
real-time quantitative pcr assay with taqman(®) probe for rapid detection of mcr-1 plasmid-mediated colistin resistance.here we report the development of two rapid real-time quantitative pcr assays with taqman(®) probes to detect the mcr-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six klebsiella pneumoniae and 12 escherichia coli) with a calibration curve that was linear from 10(1) to 10(8) dna copies. five out of 833 f ...201627489722
an updated review on the phytochemistry, pharmacology, and clinical trials of salacia oblonga.salacia oblonga (s. oblonga), a perennial herb, has been used for thousands of years in ayurvedic medicine and is closely associated with prevention, treatment, and cure of various human ailments such as obesity and diabetes. a vast and wide range of chemical compounds such as polyphenols, friedelane-type triterpenes, norfriedelane-type triterpenes, eudesmane-type sesquiterpenes including various glycosides had been isolated from this plant. this review is aimed to survey the literature covering ...201628082793
successful treatment of giant left subclavian artery pseudoaneurysm abutting the arch of the aorta and descending aorta.a 28-year-old woman presented with 3-month history of fever of unknown origin and progressively increasing cough. she was diagnosed with pulmonary tuberculosis on bronchial lavage cultures. a chest x-ray performed on follow-up showed a new opacity in the left apical area of the chest. computed tomography scan of chest showed a large 10 cm pseudoaneurysm of the left subclavian artery 1 cm from its take off from the arch of the aorta. the pseudoaneurysm was approached through a left posterolateral ...201627867459
meropenem-induced neutropenia in a neonate.postmarketing surveillance has associated meropenem with the development of hematologic abnormalities, including agranulocytosis, neutropenia, and leukopenia, but the exact incidence in children is unknown. the case describes a full-term, 26-day-old neonate admitted for a sepsis workup. she was found to have a blood culture positive for enterobacter cloacae and suspected meningitis and was initiated on meropenem 40 mg/kg/dose intravenously every 8 hours. on day 14 of antibiotic treatment, the pa ...201627713676
catheter-related blood stream infection in patients receiving long-term home parenteral nutrition: tertiary care hospital experience in saudi arabia.parenteral nutrition (pn) is a lifesaving therapy for patients with many severe conditions, including intestinal failure. some patients require long-term pn therapy, which makes home parenteral nutrition (hpn) an attractive option to improve the quality of life. among the most common and serious complications observed in these patients are catheter-related blood stream infections (crbsis). the aim of our study is to determine the frequency of crbsi among patients receiving long-term hpn.201627488325
diverse distribution of toxin-antitoxin ii systems in salmonella enterica serovars.type ii toxin-antitoxin systems (tas), known for their presence in virulent and antibiotic resistant bacterial strains, were recently identified in salmonella enterica isolates. however, the relationships between the presence of tas (ccdab and vapbc) and the epidemiological and genetic features of different non-typhoidal salmonella serovars are largely unknown, reducing our understanding of the ecological success of different serovars. salmonella enterica isolates from different sources, belongi ...201627357537
treatment of polymicrobial osteomyelitis with ceftolozane-tazobactam: case report and sensitivity testing of isolates.stenotrophomonas maltophilia is an inherently multidrug resistant (mdr) opportunistic pathogen with many mechanisms of resistance. sentry studies reveal decreasing sensitivities of s. maltophilia to trimethoprim-sulfamethoxazole and fluoroquinolones. ceftolozane-tazobactam (zerbaxa, merck & co., inc.) a novel intravenous combination agent of a third-generation cephalosporin and β-lactamase inhibitor was demonstrated to have in vitro activity against many gram-positive, gram-negative, and mdr org ...201627437155
circulating 25-hydroxyvitamin d and 1,25-dihydroxyvitamin d concentrations and postoperative infections in cardiac surgical patients: the calcitop-study.vitamin d has immunomodulatory properties and seems to reduce the risk of infections. whether low vitamin d concentrations are independent risk factors for nosocomial postoperative infections in surgical patients remains to be studied in detail.201627355377
carbapenem-resistant non-glucose-fermenting gram-negative bacilli: the missing piece to the puzzle.the non-glucose-fermenting gram-negative bacilli pseudomonas aeruginosa and acinetobacter baumannii are increasingly acquiring carbapenem resistance. given their intrinsic antibiotic resistance, this can cause extremely difficult-to-treat infections. additionally, resistance gene transfer can occur between gram-negative species, regardless of their ability to ferment glucose. thus, the acquisition of carbapenemase genes by these organisms increases the risk of carbapenemase spread in general. ul ...201626912753
influence of culture media on detection of carbapenem hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry.in this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. false-negative results were observed for oxa-25-, oxa-26-, and oxa-72-producing acinetobacter baumannii isolates grown on macconkey agar medium. the other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.201627076665
Refresh
firstprev
12345678910...
nextlast
Displaying items 601 - 700 of 8533