Publications

association of serine in position 1124 of hantaan virus glycoprotein with virulence in mice.	hantaan virus (hv) of the genus hantavirus causes a fatal disease in suckling mice following intraperitoneal or intracerebral infection. hv cl-1, which was obtained from the 76-118 strain of hv by growth in vero e6 cells, exhibited high mortality rates in mice whereas mice infected with hv cl-2 survived without any clinical signs. to determine the molecular basis for the marked difference in virulence, we compared the nucleotide sequences of the large (l), medium (m) and small (s) segments of hv ...	1994	7964639
expression strategy of the m genome segment of hantaan virus.	the medium (m) genome segment of hantaan virus encodes the envelope glycoproteins, g1 and g2, in a continuous open reading frame with a gene order of 5'-g1-g2-3' with respect to the virus-complementary sense rna. because potential translation initiation codons and amino acids constituting typical signal sequences precede both the g1 and g2 genes, we sought to determine if g1 and g2 can be expressed independently. to investigate translational requirements for g1 and g2, we constructed m segment g ...	1994	8165863
domestic cases of hemorrhagic fever with renal syndrome in the united states.	although serologic studies have identified hantaviral infection in the united states, acute disease has not been recognized. this study describes 3 cases of domestically acquired hemorrhagic fever with renal syndrome (hfrs) in the united states. infection was due to a local strain of seoul virus (baltimore rat virus). a review of the clinical features indicated a mild illness characterized by nausea, vomiting, renal and liver failure similar to hfrs described elsewhere for rat-borne viruses. fol ...	1994	7991040
immunohistochemical detection of hantaan virus antigen in renal tissue from patient with hemorrhagic fever with renal syndrome.		1994	7915405
[the detection of antibodies to the hantaan virus in the urine of patients with hemorrhagic fever with renal syndrome].	using a commercial diagnostic preparation for indirect ifa test, 135 urine samples from 50 patients with hfrs were examined at different periods of the disease. antibodies were demonstrable in all urine specimens from hfrs patients for 13 days. in 14-20 days they could be detected in half of the patients, and no antibodies could be demonstrated since the 21st day on. the results of urine examination from healthy subjects and some patients with other clinical diagnoses were negative same as contr ...	1993	7915448
infections in an alpine environment: antibodies to hantaviruses, leptospira, rickettsiae, and borrelia burgdorferi in defined italian populations.	from 1987 to 1991, a seroepidemiologic survey for antibodies to hantaviruses, leptospira, rickettsiae, and borrelia was conducted in selected italian population groups. in the mountainous areas of northeastern italy, the prevalence of antibody to hantaviruses, as detected by indirect immunofluorescent antibody (ifa) assay, was 7.1%, 4.8%, 4.3%, and 4% in 265 forestry workers, 82 rangers, 395 farmers, and 75 hunters, respectively. among 299 alpine soldiers, the prevalence was lower (0.7%). of tho ...	1993	8093990
high frequency of antibodies to hantaan virus and hepatitis c virus in chronic haemodialysis patients. coincidence or cross-reaction?	to address the question of whether there is any coincidence or cross-reaction between hepatitis c virus (hcv) and hantaan virus (both rna arboviruses), as well as to assess the frequency of antibodies to the above viruses amongst chronic haemodialysis patients in our region.	1993	8258753
belgrade virus, a cause of hemorrhagic fever with renal syndrome in the balkans, is closely related to dobrava virus of field mice.	belgrade virus is a recently described hantavirus that causes severe hemorrhagic fever with renal syndrome (hfrs) in people living in various parts of the balkan peninsula. nucleotide sequencing of the g2-encoding region in the medium (m) segment of the viral genome, reverse transcribed and amplified by the polymerase chain reaction, revealed the belgrade virus to be substantially different from hantaan virus and other major serotypes of hantavirus but identical to dobrava virus, a virus isolate ...	1993	8102632
epitope mapping studies with neutralizing and non-neutralizing monoclonal antibodies to the g1 and g2 envelope glycoproteins of hantaan virus.	epitopes recognized by three g1-specific and two g2-specific neutralizing monoclonal antibodies to hantaan virus were mapped by sequence analyses of the complete m genome segments of neutralization escape variant viruses. for each variant, we detected nucleotide sequence substitutions which resulted in a single amino acid change in either the g1 or g2 protein. serological properties of the variant viruses correlated with changes identified by nucleotide sequence analyses. to map epitopes recogni ...	1993	7504368
localization of hantaan viral envelope glycoproteins by monoclonal antibodies in renal tissues from patients with korean hemorrhagic fever h.	the role of viruses in several renal diseases is not documented clearly. the authors attempted to localize envelope glycoproteins of hantaan virus in biopsy specimens from patients with korean hemorrhagic fever (khf) as evidence of direct viral invasion of renal tissues. the authors studied sequential sections of kidney biopsy specimens from 23 of 35 patients with serologically confirmed khf diagnosed between june 1985 and december 1989. the sections were stained with the avidin-biotin-peroxidas ...	1993	7692720
[viral infections--clinical pictures and laboratory diagnosis. part 3: hantaan virus and hantaan-related viruses, hepatitis a and b].		1993	7905848
comparison of virulence between seoul virus strain sr-11 and hantaan virus strain 76-118 of hantaviruses in newborn mice.	virulence of hantavirus strain of sr-11 seoul virus and hantaan 76-118 (htn) of hantaan virus were compared. infections of both strains were lethal in newborn mice. however, inoculum required to cause lethal infection was about 4,000 times higher for strain htn (1.65 x 10(3) pfu/mouse/ld50) than for strain sr-11 (0.36 pfu). thus, both strains were considered pathogenic to newborn mice but they possessed different levels of virulence. the assay system used for these strains in newborn mice proved ...	1993	7901740
epizootiological study of hantavirus infection among rattus norvegicus in tokyo bay area, japan.	the prevalence of antibodies against hantavirus in 413 rats (rattus norvegicus) captured in eight regions of the tokyo bay area from 1983 to 1992 were examined by the indirect fluorescent antibody test. antibody-positive rats were found in tokyo port in 1983 (34.8%), 1984 (25.9%), 1985 (22.0%) and 1986 (15.6%), in kasai seaside park in 1989 (3.2%) and 1990 (4.2%) and in chiba port in 1990 (6.7%). in tokyo port, antibody-positive rats were found in any season in 1984 and 1985, and seroprevalences ...	1993	7903711
[detection of hemorrhagic fever virus-rna in renal tissue and peripheral blood in patients with hemorrhagic fever with renal syndrome].	hemorrhagic fever virus (hfv) rna in renal tissue and blood samples from 21 patients with epidemic hemorrhagic fever with renal syndrome (hfrs) were examined by either in situ or blot hybridization using digoxigenin labelled hfv-cdna probe. hfv associated antigen and immune complex (ic) in renal tissue were also investigated simultaneously using immunofluorescence (if) technique. hfv-rna was detected in the renal specimen particularly renal tubular in 8/21 hfrs patients mostly of severe type ill ...	1993	7906187
[a study on gastric mucosa damage, antigen location, and virus dissociation in patients with epidemic hemorrhagic fever].	gastric mucosa damage was investigated with techniques of gastroscopy, gastric biopsy, antigen location with immunohistochemical enzyme staining and virus dissociation in 135 patients with epidemic hemorrhagic fever (ehf). we found that the major pathologic finding in gastric mucosa is hemorrhage of squamous shape. ehf virus antigen exists in epithelial cytoplasm of the superficial layer and lamina propria of gastric mucosa and the virus is dissociated from gastric mucosa. the virus is identifie ...	1993	7907971
[advances in research on pathogenesis of epidemic hemorrhagic fever].		1993	7907974
epidemiologic surveillance on the hemorrhagic fever with renal syndrome in china.	this article summarizes the contents and results of extensive surveillance on hemorrhagic fever with renal syndrome (hfrs) in china. data from surveillance on human hfrs, including morbidity and case fatality rate, trend of epidemics, periodicity of epidemics, factors related to epidemics, types of epidemic areas, environment structure of natural nidi, geographic distribution, seasonal distribution, age distribution, sex distribution and occupation distribution, as well as surveillance on animal ...	1993	7908258
rna binding of recombinant nucleocapsid proteins of hantaviruses.	genes encoding the nucleocapsid (n) proteins of two hantaviruses, hantaan virus strain 76-118 (htn) and puumala virus strain cg 18-20 (puu), were expressed in escherichia coli as histidine-tagged proteins. they were purified by metal-chelate affinity chromatography under native or denaturing conditions to near homogeneity. the soluble form of htn n protein was associated with rna of e. coli. renatured n proteins were shown to bind in vitro transcribed rna representing the hantaviral small genomi ...	1993	8480424
protective immunity of hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins.	recombinant hantaan virus nucleocapsid protein (rnp) and recombinant envelope (renv) proteins were prepared using a baculovirus expression system to examine the role of hantaan virus structural proteins in protective immunity. passive transfer of spleen cells from mice immunized with rnp conferred partial protection or prolongation of time to death from fatal hantaan virus infection in suckling mice which were challenged with hantaan virus at 40 ld50 (survival rate: 43%) or 4 ld50 (survival rate ...	1993	8517793
the hantaan virus m-segment glycoproteins g1 and g2 can be expressed independently.	the two glycoproteins of hantaan virus (htv), g1 and g2, are encoded as a continuous single open reading frame in the m segment of the virion rna. they are believed to be synthesized contemporaneously via a polypeptide precursor which is then processed to yield two glycoproteins, both of which appear in the golgi complex of the cell. to study the properties of g1 and g2 as separate entities, we have constructed vaccinia virus recombinants which contain the sequences for each glycoprotein individ ...	1992	1548747
protective role of antigenic sites on the envelope protein of hantaan virus defined by monoclonal antibodies.	to investigate the role of hantaan virus envelope glycoprotein in infection, a panel of monoclonal antibodies (mabs) was examined in vitro with several serological tests and in vivo by passive transfer experiments in mice. an antigenic site, specific for the inhibition of infected cell focus was detected with the focus inhibition neutralization test (fint), in addition to the neutralization related antigenic sites, which were revealed by the ordinary focus reduction neutralization test (frnt). s ...	1992	1381911
hemorrhagic fever with renal syndrome in an endemic area of greece.	from 1983 to 1990, 32 patients with hemorrhagic fever with renal syndrome (hfrs) were admitted to our hospital. the diagnosis was confirmed by high igm type titers of antibodies to hantaan virus. all patients presented with serum and urine abnormalities suggesting renal involvement. serum creatinine was elevated and ranged between 1.8 and 14.3 mg/dl. proteinuria ranged between 0.5 and 6.4 g/24 h. seven patients died due to shock or hemorrhage, while 6 patients were supported by hemodialysis or p ...	1992	1357972
[atrioventricular block in 2 patients with hemorrhagic fever and renal syndrome (hantaan-virus nephropathy)].		1992	1357730
[detection of hfrs hantaan virus rna sequences by pcr].		1992	1357208
hantaan virus infection of human endothelial cells.	the primary pathophysiologic finding of the viral disease known as korean hemorrhagic fever, the etiological agent of which is hantaan virus (htv), is vascular instability. to investigate whether htv was able to infect cells derived from human vascular tissue and alter their behavior, we infected in vitro primary adult human endothelial cells from saphenous veins (hsvec). we were able to detect the presence of viral antigens in infected cells both by immunofluorescence and by western blot (immun ...	1992	1356160
histopathologic changes of the spleen in suckling rats inoculated with hantaan virus.	the purpose of this study is to delineate the histopathologic findings of the spleen after hantaan viral inoculation, which is the largest lymphoid organ in rats, and to identify the viral location by anti-hantaan virus (htnv) monoclonal antibody. all the sixty one suckling rats of less than twenty four hours of age were used. except twenty one rats of control group, twenty-five rats inoculated intracerebrally for the early change and fifteen suckling rats inoculated intramuscularly for the late ...	1992	1355973
cloning and sequencing of puumala virus sotkamo strain s and m rna segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein.	the prototype puumala virus (pv) sotkamo strain small (s) and medium (m) rna genome segments were amplified by the polymerase chain reaction (pcr), cloned and sequenced. the s segment is 1830 nucleotides long with an open reading frame coding for 433 amino acids. the identity to the pv hällnäs strain was 83% at the nucleotide and 96% at the amino acid level. the m segment in the sotkamo strain is 3616 nucleotides long and contains one open reading frame of 1148 amino acids with 83% nucleotide an ...	1992	1353107
belgrade virus: a new hantavirus causing severe hemorrhagic fever with renal syndrome in yugoslavia.	two biologically and genetically distinct hantaviruses were isolated from blood and urine specimens collected from four yugoslavian patients with clinically severe hemorrhagic fever with renal syndrome (hfrs). viral isolates from three patients, designated strains belgrade 1-3, were distinct from hantaan, seoul, puumala, and prospect hill viruses as determined by plaque-reduction neutralization tests and restriction analysis of enzymatically amplified m-segment fragments. the fourth isolate, cal ...	1992	1351508
seroprevalence of antibodies to hantaviruses and leptospires in selected italian population groups.	a study of hantaviral and leptospiral antibodies in selected population groups was performed. among high risk subjects in the rome area, hantaan antibody was found in mammalogists (10%) and dialysis patients (6%), while none of the trappers, oarsmen, river policemen and firemen studied tested positive for antibodies to hantaviruses. in occupationally-exposed subjects (farmers, rangers, lumbermen, hunters) from rural and densely forested areas of northern italian regions, the prevalence of hantaa ...	1992	1349288
haemorrhagic fever with renal syndrome: clinical aspects.	based on clinical and pathological features a typical case of haemorrhagic fever with renal syndrome passes through five phases: (1) febrile phase, (2) hypotensive phase, (3) oliguric phase, (4) diuretic phase and (5) convalescent phase. the major manifestations are fever, pain in the back and abdomen, flushed face, prostration, proteinuria, purpura and haemorrhage and acute renal failure. selective right auricular haemorrhage, marked congestion and haemorrhage in the renal medulla and necrosis ...	1992	1349230
preparation of candidate vaccinia-vectored vaccines for haemorrhagic fever with renal syndrome.	two vaccinia-vectored candidate vaccines for haemorrhagic fever with renal syndrome were prepared by inserting cdna, representing the medium (m) genome segment, or the m and the small (s) genome segments of hantaan virus into the thymidine kinase gene of the connaught vaccine strain of vaccinia virus. in the single recombinant, the m segment was placed under control of the vaccinia virus 7.5 kda promoter. in the double recombinant, the m and s segments were placed under control of the vaccinia v ...	1992	1347189
identification of hantaan virus-related structures in kidneys of cadavers with haemorrhagic fever with renal syndrome.	the etiologic agent of haemorrhagic fever with renal syndrome (hfrs), hantaan virus, was first isolated in 1976. since then numerous hantaan-like viruses have been isolated and five serotypes of hantavirus have been recognized. serological studies indicate that these viruses are globally distributed, with each serotype occurring in specific areas. hantaan virus has been intensively studied antigenically, biochemically, and genetically. however there is still a paucity of information on the patho ...	1992	1346088
effect of neutralizing monoclonal antibodies on hantaan virus infection of the macrophage p388d1 cell line.	the effect of neutralizing monoclonal antibodies (n mabs) on hantaan virus infection of macrophages was investigated using p388d1 cells, a murine macrophage cell line. mabs to the g1 protein (g1b) and the g2 protein (g2a and g2c) neutralized viral infectivity in p388d1 cells. n mabs to g1b showed much higher neutralizing potency than those to g2a and g2c. with each n mabs, two distinct effects were observed: neutralization of viral infectivity occurring at high concentrations and enhancement of ...	1992	1284283
[antibodies against the hantaan virus in italian ex-soldiers stationed in the balkans during world war ii].		1992	1283068
maturation of hantaan virus glycoproteins g1 and g2.	hantaan virus-infected vero e6 cell lysates were used for immunoprecipitation with monoclonal antibodies against glycoprotein g1 (mabg1) or g2 (mabg2). when cell lysates were prepared with buffer containing nonionic detergent, both g1 and g2 glycoproteins were precipitated with either mabg1 or mabg2. in contrast, when cell lysates were prepared with a buffer containing ionic detergents mabg1 precipitated only glycoprotein g1 and mabg2 precipitated only glycoprotein g2. heterodimers and possibly ...	1992	1604818
coexpression of the membrane glycoproteins g1 and g2 of hantaan virus is required for targeting to the golgi complex.	to study the intracellular transport and targeting to the golgi complex of the membrane glycoproteins g1 and g2 of hantaan virus, we have expressed them together and separately using recombinant vaccinia viruses. when expressed from the same recombinant vaccinia virus, g1 and g2 were localized to the golgi complex as analyzed by both immunofluorescence and subcellular fractionation. however, when the glycoproteins were expressed from separate recombinant viruses, both proteins remained in the en ...	1992	1727611
antigenic variation of european haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies.	monoclonal antibodies (mabs) against puumala (puu) virus, the aetiological agent of nephropathia epidemica, were produced by fusing activated spleen cells from a bank vole (clethrionomys glareolus) with the mouse myeloma cell line sp2/0. this novel approach, utilizing the natural vector of puu virus for hybridoma production, proved to be highly efficient, and eight stable puu virus-specific heterohybridomas were isolated and characterized. the bank vole mabs were all specific for the nucleocapsi ...	1991	1716651
molecular characterization of the prospect hill virus m rna segment: a comparison with the m rna segments of other hantaviruses.	complementary dna representing the genomic m rna segment of the prospect hill (ph) hantavirus was cloned and its nucleotide sequence determined. the ph virus m rna segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense rna with a coding capacity of 1142 amino acids. the predicted gene product of the ph virus m segment was compared with the corresponding gene products of hantaan virus strain 76-118 (hantaan), sapporo rat virus strain sr-11 (sr) an ...	1991	1840609
identification of hantavirus serotypes by testing of post-infection sera in immunofluorescence and enzyme-linked immunosorbent assays.	serum samples were collected from 27 individuals who had been infected with a member of the genus hantavirus in the netherlands or belgium during the last 15 years. these samples were tested in an immunofluorescence assay (ifa) and two enzyme-linked immunosorbent assay (elisa) systems, using different virus strains that represented each of the four recently proposed serotypes of this genus. the serum samples from 11 individuals who had been infected through contacts with laboratory rats showed t ...	1991	1901907
molecular characterization and expression of glycoprotein gene of hantavirus r22 strain isolated from rattus norvegicus in china.	a cdna containing the complete open reading frame of the m genome segment of hantavirus r22 strain isolated from rattus norvegicus in china, was amplified by polymerase chain reaction (pcr), and then cloned. the m segment is 3656 nucleotides in length with a predicted region of 3402 bases encoding a precursor glycoprotein of 1134 amino acids subsequently processed into viral glycoproteins 1 and 2 (g1 and g2). a strain comparison between r22 and sr11 (isolated from a rat in japan), and hantaan 76 ...	1991	1962503
antigenicity of hantavirus nucleocapsid proteins expressed in e. coli.	dna clones representing the small genomic segment of nephropathia epidemica virus strain hällnäs b1 (nev) and hantaan virus strain 76-118 (htv) encoding their nucleocapsid proteins were inserted into the e. coli vector pin-iii-ompa for secretion of proteins into the periplasmic space. the complete htv and nev nucleocapsid proteins and two truncated versions of the nev nucleocapsid proteins were expressed as fusion proteins. unexpectedly, all products accumulated as insoluble aggregates. most of ...	1991	1714110
effects of hantaan virus on human endothelial cells and their significance in pathogenesis of hemorrhagic fever with renal syndrome.	the effects of hantaan virus (htnv) on human endothelial cells (hecs) were investigated both in vivo and in vitro. the 76-118 strain or sr-11 strain of htnv were inoculated into hecs monolayers respectively, and the virus antigens could be detected on the seventh day of the first passage after inoculation by immunofluorescent technique. the htnv could also be isolated through cultures of vero e-6 cells. htnv particles and inclusion bodies together with various changes in the organelles were obse ...	1991	1686857
[hemorrhagic fever with renal syndrome in children].	seven patients, 4 girls and 3 boys, aged 3 to 12 years /x = 7.14/ affected by haemorrhagic fever with renal syndrome /hfrs/., were hospitalized at the university children's hospital in belgrade during the last two years /january 1988-january 1990/. the diagnosis was established on the basis of clinical features, epidemiological data and autopsy findings in one patient while in the others the diagnosis of hfrs was confirmed serologically by indirect immunoflorescence tests on vero e 6 cells. a si ...	1991	1686334
[acute nephritis caused by hantaan virus infection].		1991	1679521
[hantaan virus infection in twente].	to find out if hantavirus infection, as a cause of acute renal failure, has been overlooked in the past. to determine if hantavirus can be found in wild rodents, particularly in red bank voles (clethrionomys glareolus), living in the area.	1991	1675769
[hantaan virus infection, a seldom recognized cause of acute kidney insufficiency].		1991	1675768
[hantaan virus infection].		1991	1672850
detection of hantavirus rna in tissues of experimentally infected mice using reverse transcriptase-directed polymerase chain reaction.	detection of hantaviruses, the etiological agents of hemorrhagic fever with renal syndrome (hfrs), by virus isolation using experimental animals or cell culture is time-consuming. a more rapid but equally specific method is needed. we used a reverse transcriptase-directed polymerase chain reaction (rt-pcr) to detect hantavirus genomic sequences and compared its sensitivity with conventional virus isolation. rna, extracted by the guanidinium isothiocyanate-cesium chloride method from hantavirus-i ...	1991	1713266
coding properties of the s and the m genome segments of sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome.	three serologically distinct groups of hantaviruses have been associated with severe, moderate, and mild forms of hemorrhagic fever with renal syndrome (hfrs). to gain a better understanding of the genetic variation among these viruses, we cloned and sequenced the m and the s genome segments of sapporo rat virus, an etiologic agent of moderate hfrs, and compared the predicted gene products to those of hantaan virus, and the hällnäs strain of puumala virus, which are etiologic agents of severe an ...	1990	1970443
[a case of acute myopia with angle-closure glaucoma].	the authors report a case of transient myopia with closed angle following the administration of aspirin and deturgylone. this treatment was prescribed because of a probably hantaan virus infection.	1990	1972038
rapid detection of genomic variations in different strains of hantaviruses by polymerase chain reaction techniques and nucleotide sequence analysis.	the polymerase chain reaction (pcr) with subsequent nucleotide sequence analysis was employed to rapidly detect genomic variations among different hantavirus strains. using synthetic oligonucleotide primers derived from the m and s segment rnas of nephropathia epidemica virus strain hällnäs b1 (nev) we succeeded in amplifying the corresponding sequences of hantaan and puumala viruses. the nucleotide sequences of the cdnas derived from the puumala m and s rna segments were analyzed. it was found ...	1990	2385957
[hantaan virus infections].		1990	2219526
molecular mechanisms of action of ribavirin.	ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) is a broad-spectrum antiviral agent whose molecular mode of action remains remarkably controversial. this antiviral agent was approved by the u.s. food and drug administration in 1986 for use as an aerosol for infants with serious infections due to respiratory syncytial virus. ribavirin is and has been under clinical investigation for activity against a variety of viral illnesses, including those due to influenza virus, lassa fever ...	1990	2267489
nucleotide sequence analysis of the s genomic segment of prospect hill virus: comparison with the prototype hantavirus.	the genomic s rna segment of prospect hill virus (phv), a member of the hantavirus genus, was molecularly cloned and the nucleotide sequence of the cdna determined. the phv s rna segment is 1675 nucleotides long. a long open reading frame was identified in the viral complementary-sense rna that could encode a 433 amino acid (49k) nucleocapsid (n) protein. comparison with the sequence of the related hantavirus (hantaan 76-118) s rna segment indicated that there was 57% nucleotide sequence homolog ...	1990	2309440
coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae).	the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ...	1990	2327076
[the role of hantaan virus serotypes in the etiology of hemorrhagic fever with renal syndrome in the far east of the ussr].	the employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (hfrs). proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and cl. rufocanus were obtained. no data on any association of human hfrs cases with strains of serotype 4 isolated from reed vo ...	1990	1982032
molecular characterization of the rna s segment of nephropathia epidemica virus strain hällnäs b1.	the s segment rna of nephropathia epidemica virus (nev) strain hällnäs b1 was isolated by molecular cloning of the corresponding cdna. the rna is 1785 nucleotides long with the 3' and 5' termini being complementary for 23 bases. the viral messenger-sense rna contains one major open reading frame (orf) with a coding capacity of 433 amino acids encoding a 49-kda polypeptide. compared to the hantaan s segment cdna sequence there is a nucleotide homology of 60 and 61% at the amino acid level. many o ...	1990	2104684
isolation of a strain of hantaan virus from peritoneal exudate cells of a patient with hemorrhagic fever with renal syndrome.	a new strain of hantaan virus (htnv), gh716, was isolated from the peritoneal exudate cells (pec) of a patient with severe hemorrhagic fever with renal syndrome (hfrs). the isolate was propagated in vero e6 cells. at each passage the virus-infected cells were examined for htnv with immunofluorescence technique using monoclonal antibodies (mcab) 25-1 and 84-1 against htnv. from passage 5 on, fluorescent intensity tended to stabilize at (+++) and infectivity titer reached 10(5) tcid50/ml. using 14 ...	1990	1976488
[hantaan virus infection].		1990	1720000
[hantaan virus infection--also in germany a cause of acute renal failure].		1990	1971417
hemorrhagic fever with renal syndrome. separation of human peripheral blood t and b cells and detection of viral antigen.	in this study, total blood lymphocytes were prepared from patients with hemorrhagic fever with renal syndrome (hfrs) by a density gradient on ficoll-hypaque. t and b cells were then purified by passing the total lymphocytes over a nylon wool column. the purities of total lymphocytes, t cells and b cells were 97.8 +/- 2.3%, 91.6 +/- 4.5% and 74.2 +/- 12.1%, respectively. also, after modification of cell fixation and smear drying, the number of cells were increased and the time needed for slide pr ...	1990	1972048
antigenic subunits of hantaan virus expressed by baculovirus and vaccinia virus recombinants.	baculovirus and vaccinia virus vectors were used to express the small (s) and medium (m) genome segments of hantaan virus. expression of the complete s or m segments yielded proteins electrophoretically indistinguishable from hantaan virus nucleocapsid protein or envelope glycoproteins (g1 and g2), and expression of portions of the m segment, encoding either g1 or g2 alone, similarly yielded proteins which closely resembled authentic hantaan virus proteins. the expressed envelope proteins retain ...	1990	1972201
the protean manifestations of hemorrhagic fever with renal syndrome. a retrospective review of 26 cases from korea.	twenty-six cases of hemorrhagic fever with renal syndrome from 1981 to 1986 were retrospectively reviewed to determine the scope of clinical presentation and the unique complications of the illness. the diagnosis was confirmed by detection of hantaan virus antibody in 25 cases and by characteristic autopsy findings in 1 case. the illness could be classified into three distinct clinical subgroups. fever was universally present. two patients presented with intractable shock and diffuse hemorrhage ...	1990	1974404
[the serological diagnosis of hantaan virus infections].		1990	1977569
[the clinical picture and therapy of hantaan virus infection].		1990	1977570
a retrospective analysis of sera collected by the hemorrhagic fever commission during the korean conflict.	more than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the hemorrhagic fever commission during the korean conflict, 1951-1954. these sera were tested for igm- and igg-specific antibodies to hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both hantaan and seoul viruses. only 15 patients failed to develop antihantaviral antibodies; most sera ...	1990	1977805
[hantaan virus infections].		1990	1979455
[hantaan virus serotypes circulating in foci of the far eastern region of the ussr].	studies on serodifferentiation by indirect immunofluorescence antibody method and neutralization test of hantaan virus strains isolated from human patients and rodents revealed circulation on the maritime territory of 5 virus serotypes and demonstrated that rodents of one species could be virus carriers of different virus serotypes. on the basis of the antigenic similarity of strains isolated from human patients, field mice, and clethrionomys rufocanus it is concluded that the virus serotypes ci ...	1990	1980045
different pathohistological presentations of acute renal involvement in hantaan virus infection: report of two cases.	we present two patients with hantaan virus infection, admitted to the department of nephrology, skopje, at the same time, with the same clinical presentation (chills, fever, abdominal pain, hemorrhages, nausea, headache, proteinuria, hematuria, oliguria, acute renal failure) but with different pathohistological findings and different disease courses. in the first case diffuse proliferative glomerulonephritis was found, with a complete recovery of renal function after a month, with a mild protein ...	1990	1980098
nucleotide sequence of the l genome segment of hantaan virus.		1990	2123544
[an electron microscopic study of viral infected peripheral mononuclear cells in epidemic hemorrhagic fever].	ultrathin sections of peripheral blood cells from 7 patients of early stage epidemic hemorrhagic fever (ehf) were reexamined under transmission electron microscope. virus particles were found in the cytoplasmic vacuoles of mononuclear cells from two patients. the morphology and morphogenesis of the matured virus were identical with those of hantaan virus of the bunyaviridae family. additionally, abnormal aggregations of tubuloreticular structure (trs) were sometimes encountered in the dilated ci ...	1989	2576865
characterization of hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies.	a panel of 24 monoclonal antibodies (mabs) to the g1 or g2 envelope glycoproteins of hantaan virus were used to determine the surface topography and functional properties of antigenic sites. nine distinct, partially overlapping antigenic sites, two on g1 and seven on g2, were demonstrated by competitive binding assays. analyses of the antigenic sites by haemagglutination (ha) inhibition and plaque-reduction neutralization tests showed that all of the sites, except one on g1, were related to vira ...	1989	2471792
prospects for treatment of viral hemorrhagic fevers with ribavirin, a broad-spectrum antiviral drug.	ribavirin, a broad-spectrum antiviral drug, is active against hemorrhagic fever viruses (with the exception of ebola virus) in cell culture systems. in model infections with arenaviruses in guinea pigs and monkeys, ribavirin has demonstrated both prophylactic and therapeutic efficacy. in therapeutic studies it has not prevented late-onset neurologic disease. in human cases of lassa fever, it significantly reduces mortality when administered before day 7 of illness to persons at high risk. in rod ...	1989	2546248
comparison of immunofluorescence and enzyme-linked immunosorbent assays for the serology of hantaan virus infections.	three enzyme-linked immunosorbent assay (elisa) systems based upon different principles were developed for the serology of hantaan virus infections and compared with an indirect immunofluorescence assay (ifa). the indirect ifa was carried out with gamma-irradiated hantaan virus-infected and uninfected vero e6 cells fixed with ethanol (-70 degrees c) or acetone (20 degrees c) on drop slides and a fitc-coupled sheep anti-human ig preparation. atypical staining in the ifa was avoided by using ethan ...	1989	2566619
[antibodies against hantaan virus and leptospira in subjects at risk in rome].	a survey on the prevalence of hantaan and leptospiral antibodies on mammalogists and rodent control personnel was performed. none of the 66 trappers studied (using ifi ) had detectable hantaan antibody, while only 2 out of 20 mammalogists presented antibody at low titer (1:32). for leptospiral antibody the microagglutination test (mat) using live leptospires as antigen was performed. 14 out of 66 trappers, or 21.2 per cent, had antibodies, at titer of 1:50 or more, to various leptospiral serovar ...	1989	2518821
immunoblot analysis of the serological response in hantavirus infections.	sera from patients with nephropathia epidemica (ne) or korean hemorrhagic fever (khf) were tested for specific antibody response to antigens of hällnäs virus and hantaan virus strain 76-118. a vero e6 derived cell line persistently infected with hällnäs virus strain b1, and vero e6 cells freshly infected with hantaan virus type strain 76-118 were used as antigens in the immunofluorescence assay (ifa) and the immunoblot. blots were prepared from whole cell lysates. the convalescent-phase sera of ...	1989	2566644
serological studies of haemorrhagic fever with renal syndrome (hfrs) in poland. preliminary report.	serological studies of hantaan virus infections among--blood donors (control group)--patients: chronic dialysed, leptospirosis suspected, hospitalised for various diseases,--risk group population: farmers, laboratory staff (contact with laboratory animals), as well as laboratory and wild animals (rodents) were carried out. surveys were done by the indirect immunofluorescent antibody (ifa) procedure with 5 hantaan virus strains as antigens.	1989	2476002
antibody prevalence against haemorrhagic fever viruses in randomized representative central african populations.	between 1985 and 1987, 5,070 randomly selected persons living in 6 central african countries (cameroon, central african republic, chad, congo, equatorial guinea and gabon) were checked for serological evidence of haemorrhagic fever. rural and urban areas were studied, including ecoclimatic zones ranging from dry savana to tropical rain forest. virus-reactive antibodies were found with all antigens tested, and the global prevalence of positive sera was distributed as follows: crimean-congo haemor ...	1989	2505350
determination of the coding capacity of the m genome segment of nephropathia epidemica virus strain hällnäs b1 by molecular cloning and nucleotide sequence analysis.	the m genome rna segment of nephropathia epidemica virus (nev) strain hällnäs b1 was characterized by molecular cloning and dna nucleotide sequencing of the corresponding cdna clones. the size of the m rna segment is 3682 nucleotides. the 3' and 5' terminal sequences are complementary for 21 bases and their predicted secondary structure is very stable. the viral complementary messenger rna possesses a single long open reading frame with a coding capacity of 1148 amino acids (polypeptide of 126 k ...	1989	2508317
cross-reactive immunity among different serotypes of virus causing haemorrhagic fever with renal syndrome.	spleen cells primed by prospect hill (ph) or puumala (pu) virus could cross-react with hantaan virus (hv) 76-118 strain-infected target cells after in vitro stimulation with hv-infected cells, although anti-ph or anti-pu immune serum showed no cross-reactive neutralizing (nt) activity to hv without complement. these results and our previous findings with cross-reactive cytotoxic t lymphocytes (ctls) suggest that some epitopes recognized by ctls might be common among the hantavirus genus, while t ...	1989	2567343
the minor problem of hemostatic impairment in nephropathia epidemica, the mild scandinavian form of hemorrhagic fever with renal syndrome.	nephropathia epidemica (ne) is the mild scandinavian form of hemorrhagic fever with renal syndrome (hfrs). its hemorrhagic manifestations are so mild that initially--as its name implies--it was not considered a hemorrhagic fever. it is, however, clinically, histologically, and epidemiologically similar to korean hemorrhagic fever (khf) except that hemorrhagic manifestations are fewer and mortality is lower. the etiologic agents of the two disorders, puumala virus (ne) and hantaan virus (khf), ar ...	1989	2568675
hemorrhagic fever with renal syndrome in korea.	several clinical variants of hemorrhagic fever with renal syndrome (hfrs) are caused by hantaan and related viruses. since 1951, 500-900 patients with hfrs have been hospitalized annually in korea. although hfrs is associated primarily with rural areas, it is now being recognized as an urban problem and a particular hazard to laboratory staff using rodents for research. recently, epidemic outbreaks of leptospirosis and scrub typhus have occurred during the hfrs season, leading to confusion in di ...	1989	2568676
the distribution and duration of hantaan virus in the body fluids of patients with hemorrhagic fever with renal syndrome.	the distribution and duration of hantaan virus (htnv) in the body fluids of patients were studied by immunofluorescence, reverse passive hemagglutination, and cell culture assays. virus antigen of hemorrhagic fever with renal syndrome in peripheral blood mononuclear cells (pbmcs) was usually present before day 11 of the disease, especially from days 4-7. virus isolates were more readily recovered from plasma early in the course of the illness and less frequently after day 7. the use of pbmcs rat ...	1989	2569493
hemorrhagic fever with renal syndrome in yugoslavia: antigenic characterization of hantaviruses isolated from apodemus flavicollis and clethrionomys glareolus.	hantavirus antigens were detected in lung tissues of 8/113 apodemus flavicollis and 2/17 clethrionomys glareolus captured in 1984 in fojnica, a region of yugoslavia endemic for hemorrhagic fever with renal syndrome; hantavirus antigens were not detected in lung tissues from 126 other mammals collected in fojnica. three hantaviruses, 2 from a. flavicollis and 1 from c. glareolus, were isolated directly in vero e6 cells and were partially characterized. the isolates from a. flavicollis, designated ...	1989	2569846
[another case of hantaan virus infection in the netherlands].	in a serological survey among dutch patients suspected of leptospirosis, using a recently developed enzyme-linked immunosorbent assay, a patient was traced with a high antibody titre to hantaan virus. no anti-leptospira antibodies were detected in this 27-year-old man. shortly before he had been admitted to the hospital with progressive dyspnoea and coughing, accompanied with high fever. an interstitial pneumonia was diagnosed. he subsequently developed a progressive renal failure with proteinur ...	1989	2572978
pathogenesis of hantaan virus in mice.	the virulence of two virus clones (hv cl-1 and hv cl-2) of hantaan virus, which were plaque-purified on vero e6 cells, were compared in suckling mice infected by the intraperitoneal or intracerebral route. hv cl-1 increased the mortality of the mice whereas hv cl-2 did not. furthermore, virus of high titre was isolated from various organs of mice infected with hv cl-1 and high titres were maintained, whereas after infection with hv cl-2, virus was isolated from various organs only in low titre a ...	1989	2573685
[the incidence and clinical picture of hemorrhagic fever with renal syndrome in east slovakia: the eastern and western type].	hemorrhagic fever with renal syndrome (hfrs) is endemic in east slovakia. the paper presents the clinical picture of 4 serologically confirmed cases. two of the 4 patients had serological evidence of recent hantaan virus infection. positive immunofluorescent reactions with puumala virus (cg 18-20) were detected in the sera of the other two patients. the obtained results suggest the circulation of two serotypes of hfrs in slovakia.	1989	2574625
[sero-epidemiological study in mauritania (1985-1986): incidence of treponematosis, hepatitis b virus, hiv virus and viral hemorrhagic fevers].	a serological serosurvey was made in different ethnic groups of mauritania in 1985. a very high prevalence of hepatitis b markers was found with more than 20% of hbs antigen carriers. treponema specific antibodies in low-age classes observed is a reflect of endemic syphilis. the seroprevalence of antibody against hiv and viral haemorrhagic fever viruses (rift valley fever, crimean-congo haemorrhagic fever and haemorrhagic fever with renal syndrome) was very low.	1988	2900081
conservation of antigenic properties and sequences encoding the envelope proteins of prototype hantaan virus and two virus isolates from korean haemorrhagic fever patients.	viruses isolated from the blood of two korean haemorrhagic fever patients were propagated in cell culture and compared to prototype hantaan virus which was isolated from apodemus mice. the antigenic properties of the human isolates were found to be closely related to hantaan virus by plaque reduction neutralization, haemagglutination inhibition and fluorescent antibody staining with both polyclonal and monoclonal antibodies. the medium genome segment of each human isolate was sequenced and compa ...	1988	2900289
hemorrhagic fever with renal syndrome in yugoslavia: detection of hantaviral antigen and antibody in wild rodents and serological diagnosis of human disease.	lung tissues from 547 rodents and 26 insectivores captured between 1981 and 1984 in central bosnia (fojnica) and central serbia (cacak), 2 regions known to be endemic for hemorrhagic fever with renal syndrome (hfrs), were examined for hantaviral antigen by the indirect immunofluorescent antibody technique. antigen was detected in 17/231 apodemus flavicollis, 3/187 a. sylvaticus, 1/46 a. agrarius, 4/32 mus musculus, and 3/28 clethrionomys glareolus. in addition, antibodies against hantaan and puu ...	1988	2900550
identification of the antigenic components of hantaan virus reacting with rabbit antisera and sera from patients with hemorrhagic fever with renal syndrome.		1988	2897400
enzyme immuno assay for the detection of virus specific igg and igm antibody in patients with haemorrhagic fever with renal syndrome.	consecutive serum samples collected from 235 patients with haemorrhagic fever with renal syndrome (hfrs), between two days and two years after onset of disease, have been analysed for the presence of igg and igm type of antibodies specific for hanta-viruses. the sera were screened in parallel by a newly developed indirect immuno enzyme assay (eia) in parallel with indirect immunofluorescent antibody assay (ifa). in both tests the hantaan virus strain 76-118 was used as the antigen. the eia was m ...	1988	2898929
hemorrhagic fever with renal syndrome (nephropathia epidemica) in norway: seroepidemiology 1981-1985.	an indirect fluorescent antibody technique (ifat) was applied to serologically confirm the clinical diagnosis in 507 nephropathia epidemica (ne) suspected patients. hantaan virus (hv), the agent of korean hemorrhagic fever, which is serologically related to the ne agent, was used as antigen. both igg and igm reactions were detected. high levels of igg antibodies to hv were common, even in the acute phase of illness. over a 5-year period, a total of 35% of the ne suspected patients revealed antib ...	1988	2900551
seroepidemiology of haemorrhagic fever with renal syndrome in bulgaria.	during the period of 1954-1986, 399 cases of haemorrhagic fever with renal syndrome (hfrs) were registered in bulgaria with 63 (15.7%) deaths. three hundred serum samples from 214 patients who had contracted the disease from 1957 to 1986 were investigated by indirect fluorescent antibody test (if-at). as antigen vero-e6 cells infected with the asian hantaan virus were used, as well as lung sections from bank voles (clethrionomys glareolus) infected with strains udmurt and kazan 6-cg from the eur ...	1988	2902774
serological evidence of hantavirus infection in laboratory rats and personnel.	laboratory-acquired haemorrhagic fever with renal syndrome (hfrs) has been reported in many countries. a serological survey of laboratory white rats and of laboratory personnel for antibodies to hantaviruses was conducted in singapore. forty-four per cent (143/329) of rats were seropositive by the indirect immunofluorescent antibody test but none had hantaviral antigens in lung tissues. two of 74 laboratory personnel were seropositive but neither had a history of clinical illness. the high serop ...	1988	2906333
expression of the hantaan virus m genome segment by using a vaccinia virus recombinant.	a cdna containing the complete open reading frame of the hantaan virus (htn) m genome segment has been cloned into vaccinia virus. this recombinant virus expresses two glycoproteins which are similar to the htn structural glycoproteins, g1 and g2, in molecular weight, cleavage pattern, and cellular distribution. both htn and recombinant vaccinia virus glycoproteins are exclusively associated with the golgi apparatus of the cell. despite this intracellular restriction, mice inoculated with the re ...	1988	3123711
incidence and geographic distribution of serologically verified cases of nephropathia epidemica in sweden.	a nationwide survey of hospitalized, serologically verified adult nephropathia epidemica cases in sweden from 1978 to 1981 was performed. serum was collected from 1,063 patients with a diagnosis of nephropathia epidemica or one of 10 other renal diseases with related symptoms. sera were analyzed by indirect immunofluorescence technique for the presence of immunoglobulin g antibodies to hantaan virus, causative agent of korean hemorrhagic fever that cross-reacts with the nephropathia epidemica ag ...	1988	2895579
[hantaan virus infections as a cause of acute kidney failure. 3 cases in west germany].	three cases of hantaan virus infection (korean haemorrhagic fever) leading to acute renal failure are described. all three had mild haemorrhagic fever with a renal syndrome. it had started with acute fever followed by oliguria, proteinuria and microhaematuria (in two patients) in the further course of the disease, as well as urea and creatinine retention. one patient needed to be dialysed twice. hantaan virus-specific igg antibodies were demonstrated in all three patients; one also had igm antib ...	1988	2897900
[propagation and replication of hantaan virus in human endothelial cells in vitro].		1988	3147808
cell-mediated immunity to virus causing haemorrhagic fever with renal syndrome: generation of cytotoxic t lymphocytes.	cytotoxic t lymphocytes (ctls) were generated when spleen cells from mice infected with viruses causing haemorrhagic fever with renal syndrome were stimulated in vitro with syngeneic cells infected with viruses. these cytotoxic effector cells, with lyt2+ l3t4- markers on their surface, demonstrated h-2 restriction. ctls induced by hantaan virus (76-118 strain) or seoul virus (b-1 strain) showed cross-reactivity with infected target cells. hantaan virus infection induced a higher ctls response th ...	1988	3137309