Publications

densin-180, a synaptic protein, links to psd-95 through its direct interaction with maguin-1.	densin-180, a brain-specific protein highly concentrated at the postsynaptic density (psd), belongs to the lap [leucine-rich repeats and psd-95/dlg-a/zo-1 (pdz) domains] family of proteins, some of which play fundamental roles in the establishment of cell polarity.	2002	12390249
filamin a and filamin b are co-expressed within neurons during periods of neuronal migration and can physically interact.	mutations in the x-linked gene filamin a (flna) lead to the human neurological disorder, periventricular heterotopia (ph). although ph is characterized by a failure in neuronal migration into the cerebral cortex with consequent formation of nodules in the ventricular and subventricular zones, many neurons appear to migrate normally, even in males, suggesting compensatory mechanisms. here we characterize expression patterns for flna and a highly homologous protein filamin b (flnb) within the nerv ...	2002	12393796
recombinant human heparin-binding neurite-promoting factor expressed with yeast stimulates neurites outgrowth.	heparin-binding neurite-promoting factor (hbnf) is a heparin-binding protein primarily found in the brain, which can stimulate neurite outgrowth in vitro. we expressed recombinant human heparin-binding neurite-promoting factor (hrhbnf) using a yeast system, and observed its activity in stimulating neurite outgrowth in vitro.	2002	12411111
vigilant vectors: adeno-associated virus with a biosensor to switch on amplified therapeutic genes in specific tissues in life-threatening diseases.	there are many life-threatening and chronic diseases in which physiological signals could be used to switch on therapeutic protective genes. we are developing a gene therapy approach in which a systemically injected "vigilant vector" waits for these signals and switches on genes to protect specific tissues with high amplification. the concept of a vigilant vector requires four components. the first component is a safe and stable vector that can be administered by systemic injection and express t ...	2002	12413425
thyroid hormone action is disrupted by bisphenol a as an antagonist.	bisphenol a (bpa), a monomer of polycarbonate plastics, has been shown to possess estrogenic properties and act as an agonist for the estrogen receptors. although an epidemiologically based investigation has suggested that some chemicals could disrupt thyroid function in animals, the effects on thyroid hormone receptors (trs) are unknown. we show here that bpa inhibits tr-mediated transcription by acting as an antagonist. in the transient gene expression experiments, bpa suppressed transcription ...	2002	12414890
expression and genomic organization of the zebrafish chaperonin gene complex.	chaperonin 10 and chaperonin 60 monomers exist within the multimeric mitochondrial chaperonin folding complex with a stoichiometry of 2:1. this complex is located in the mitochondrial matrix, where it aids in the folding and acquisition of the tertiary structure of proteins. we have previously isolated the cpn10 cdna in zebrafish (danio rerio), and demonstrated that it is ubiquitously expressed during embryonic development and transcriptionally upregulated after exposure to heat shock. in the pr ...	2002	12416612
evidence for the presence of habp1 pseudogene in multiple locations of mammalian genome.	the gene encoding hyaluronan-binding protein 1 (habp1) is expressed ubiquitously in different rat tissues, and is present in eukaryotic species from yeast to humans. fluorescence in situ hybridization indicates that this is localized in human chromosome 17p13.3. here, we report the presence of homologous sequences of habp1 cdna, termed processed habp1 pseudogene in humans. this is concluded from an additional pcr product of ~0.5 kb, along with the expected band at approximately 5 kb as observed ...	2002	12443542
regulation of internal ribosome entry site-mediated translation by eukaryotic initiation factor-2alpha phosphorylation and translation of a small upstream open reading frame.	adaptation to amino acid deficiency is critical for cell survival. in yeast, this adaptation involves phosphorylation of the translation eukaryotic initiation factor (eif) 2alpha by the kinase gcn2. this leads to the increased translation of the transcription factor gcn4, which in turn increases transcription of amino acid biosynthetic genes, at a time when expression of most genes decreases. here it is shown that translation of the arginine/lysine transporter cat-1 mrna increases during amino a ...	2002	11684693
correlations between mrna expression levels and gc contents of coding and untranslated regions of genes in rodents.	gene expression is regulated by a highly coordinated network of events whose efficiency may constrain the level of expression. among other factors, natural selection for increased translational efficiency and/or fidelity may shape nucleotide composition and, hence, codon usage during evolution. previous studies have shown that highly expressed genes in saccharomyces cerevisiae, caenorhabditis elegans, and drosophila melanogaster have relatively higher codon usage biases. however, in the case of ...	2002	11734896
functional expression of heteromeric calcitonin gene-related peptide and adrenomedullin receptors in yeast.	the ability of g protein-coupled receptors (gpcrs) to form homo- and heteromeric complexes has important implications for the regulation of cellular events. a notable example of heteromer formation is the interaction of the calcitonin receptor-like receptor (crlr) with different members of the receptor activity modifying protein (ramp) family, which results in the formation of two different receptors, a calcitonin gene-related peptide (cgrp) receptor and an adrenomedullin receptor. to analyze th ...	2002	11733510
artifactual uncoupling by uncoupling protein 3 in yeast mitochondria at the concentrations found in mouse and rat skeletal-muscle mitochondria.	western blots detected uncoupling protein 3 (ucp3) in skeletal-muscle mitochondria from wild-type but not ucp3 knock-out mice. calibration with purified recombinant ucp3 showed that mouse and rat skeletal muscle contained 0.14 microg of ucp3/mg of mitochondrial protein. this very low ucp3 content is 200-700-fold less than the concentration of ucp1 in brown-adipose-tissue mitochondria from warm-adapted hamster (24-84 microg of ucp1/mg of mitochondrial protein). ucp3 was present in brown-adipose-t ...	2002	11743882
nuclear localization of duplin, a beta-catenin-binding protein, is essential for its inhibitory activity on the wnt signaling pathway.	duplin binds to beta-catenin and inhibits the wnt signaling pathway, thereby leading to repression of the beta-catenin-mediated transactivation and xenopus axis formation. to find an additional function of duplin, yeast two-hybrid screening was carried out. importin alpha was isolated as a binding protein of duplin. importin alpha bound directly to basic amino acid clusters of duplin. although duplin was present in the nucleus, deletion of the basic amino acid clusters (duplin(delta 500-584)) re ...	2002	11744694
hsc/hsp70 interacting protein (hip) associates with cxcr2 and regulates the receptor signaling and trafficking.	the ligand-induced trafficking of chemokine receptors plays a significant role in the regulation of inflammatory processes and human immunodeficiency infection. although many chemokine receptors have been demonstrated to internalize through clathrin-coated vesicles, a process that involves the binding of arrestins to the receptors, accumulating evidence has suggested the possible existence of other regulators. in a yeast two-hybrid screening using the c-terminal domain of cxcr2 as a bait, the hs ...	2002	11751889
functional assays for mammalian g-protein-coupled receptors in yeast.		2002	11771426
phosphorylation states of cdc42 and rhoa regulate their interactions with rho gdp dissociation inhibitor and their extraction from biological membranes.	the rho gdp dissociation inhibitor (rhogdi) regulates the activation-inactivation cycle of rho small gtpases, such as cdc42 and rhoa, by extracting them from the membrane. to study the roles of mg(2+), phosphatidylinositol 4,5-bisphosphate (pip(2)), ionic strength and phosphorylation on the interactions of rhogdi with cdc42 and rhoa, we developed a new, efficient and reliable method to produce prenylated rho proteins using the yeast saccharomyces cerevisiae. it has been previously reported that ...	2002	11772396
phosphorylation of stargazin by protein kinase a regulates its interaction with psd-95.	stargazin is the first transmembrane protein known to associate with ampa (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate) glutamate receptors (ampars) and regulate their synaptic targeting by two distinct mechanisms, specifically via delivery of ampars to the surface membrane and synaptic targeting of these receptors by binding to psd-95/sap-90 and related pdz proteins. however, it is not known whether and how this stargazin-mediated synaptic targeting of ampars is regulated. stargazin in ...	2002	11805122
functional evidence for a metastasis suppressor gene for rat prostate cancer within a 60-kilobase region on human chromosome 8p21-p12.	we recently demonstrated that the human chromosome 8p21-p12 region encodes a metastasis suppressor gene for rat prostate cancer. the presence of this region suppresses the metastatic ability of rat prostate cancer cells (n. nihei et al., genes chromosomes cancer, 17: 260-268, 1996). to define further the region harboring the metastasis suppressor gene, a truncated human chromosome 8 containing this region was transferred into highly metastatic at6.3 rat prostate cancer cells by microcell-mediate ...	2002	11809681
purification of the type ii and type iii isozymes of rat hexokinase, expressed in yeast.	c-terminally his-tagged versions of the type ii and type iii isozymes of rat hexokinase were expressed in pichia pastoris and schizosaccharomyces pombe, respectively. milligram amounts of the homogeneous isozymes were readily obtained in good yield by chromatography on ni-nta columns. the specific activities were 133 +/- 4 and 76 +/- 3 u/mg for the purified type ii and type iii isozymes, respectively. the k(m)'s for glucose and atp were in good agreement with values in the literature for the iso ...	2002	11812227
interaction of the heart-specific lim domain protein, fhl2, with dna-binding nuclear protein, hnp220.	using a yeast two-hybrid library screen, we have identified that the heart specific fhl2 protein, four-and-a-half lim protein 2, interacted with human dna-binding nuclear protein, hnp220. domain studies by the yeast two-hybrid interaction assay revealed that the second lim domain together with the third and the fourth lim domains of fhl2 were responsible to the binding with hnp220. using green fluorescent protein (gfp)-fhl2 and blue fluorescent protein (bfp)-hnp220 fusion proteins co-expressed i ...	2002	11813260
the activating enzyme of nedd8 inhibits steroid receptor function.	coregulator proteins, coactivators and corepressors, have a profound influence on steroid receptor activity and play a role in regulating receptor levels. to identify novel coregulators of nuclear receptors, we used the ligand-binding and hinge region of eralpha as bait in a yeast two-hybrid screen of a cdna library derived from rat uterine luminal epithelium. we report the cloning and characterization of a cdna encoding a protein homologous to yeast and human ubiquitin-activating enzyme 3 (uba3 ...	2002	11818503
identification of novel point mutations in erk2 that selectively disrupt binding to mek1.	extracellular signal-regulated kinases 1 and 2 (erk1 and erk2) are essential components of pathways through which signals received at membrane receptors are converted into specific changes in protein function and gene expression. as with other members of the mitogen-activated protein (map) kinase family, erk1 and erk2 are activated by phosphorylations catalyzed by dual-specificity protein kinases known as map/erk kinases (meks). meks exhibit stringent specificity for individual map kinases. inde ...	2002	11823456
structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from bacillus stearothermophilus with broad specificity phosphatase activity.	the crystal structure of bacillus stearothermophilus phoe (originally termed yhfr), a broad specificity monomeric phosphatase with a molecular mass of approximately 24 kda, has been solved at 2.3 a resolution in order to investigate its structure and function. phoe, already identified as a homolog of a cofactor-dependent phosphoglycerate mutase, shares with the latter an alpha/beta/alpha sandwich structure spanning, as a structural excursion, a smaller subdomain composed of two alpha-helices and ...	2002	11827481
wich, a novel verprolin homology domain-containing protein that functions cooperatively with n-wasp in actin-microspike formation.	we describe a novel protein that contains a verprolin-homology (v) region, through which several actin-regulating proteins, including wiskott-aldrich syndrome protein (wasp) family members, bind directly to actin. the amino acid sequence is homologous to the sequences of wasp-interacting protein (wip) and cr16, both of which associate with wasp and/or n-wasp, and thus these three proteins constitute a new protein family. we named the protein wich (wip- and cr16-homologous protein). wich associat ...	2002	11829459
identification of rat emap, a delta-glutamate receptor binding protein.	while most subtypes of glutamate receptors have been studied extensively, less is known about the delta-glutamate receptors, delta1 and delta2. although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of delta2. we used the cytosolic c-terminus of the delta2 glutamate receptor subunit in a yeast two-hybrid screen of a rat brain cdna library to identify delta-glutamate receptor binding proteins. we isolated ...	2002	11829466
interaction with grp58 increases activity of the thiazide-sensitive na-cl cotransporter.	the thiazide-sensitive sodium-chloride cotransporter (ncc) is expressed by distal convoluted tubule cells of the mammalian kidney. we used yeast two-hybrid screening to identify that glucose-regulated protein 58 (grp58), a protein induced by glucose deprivation, binds to the cooh terminus of the ncc. immunoprecipitation of rat kidney cortex homogenates using a guinea pig anti-ncc antibody confirmed that grp58 associates with the ncc in vivo. northern blots indicated that grp58 is highly expresse ...	2002	11832422
role of adaptor complex ap-3 in targeting wild-type and mutated cd63 to lysosomes.	cd63 is a lysosomal membrane protein that belongs to the tetraspanin family. its carboxyterminal cytoplasmic tail sequence contains the lysosomal targeting motif gyevm. strong, tyrosine-dependent interaction of the wild-type carboxyterminal tail of cd63 with the ap-3 adaptor subunit mu 3 was observed using a yeast two-hybrid system. the strength of interaction of mutated tail sequences with mu 3 correlated with the degree of lysosomal localization of similarly mutated human cd63 molecules in sta ...	2002	11907283
mutations in conserved regions 1, 2, and 3 of raf-1 that activate transforming activity.	to investigate the role of raf-1 in v-ha-ras transformation, we have isolated and characterized a number of raf-1 mutants that display increased transforming activity in rat2 fibroblasts. a dipeptide deletion (delta144-145) in the cysteine-rich domain (crd) of conserved region (cr) 1 increased the interaction between raf-1 and v-ha-ras effector loop mutants in the yeast two-hybrid system, supporting the proposal that the crd serves as a secondary ras-binding domain. many activating mutations wer ...	2002	11933072
subunit specificity and interaction domain between gaba(a) receptor-associated protein (gabarap) and gaba(a) receptors.	gabarap (gaba(a) receptor-associated protein) interacts with both microtubules and gaba(a) receptors in vitro and in vivo and is capable of modulating receptor channel kinetics. in this study, we use the intracellular loop of 15 gaba(a) receptor subunits to show that the interaction between gabarap and gaba(a) receptor is specific for the gamma subunits. pharmacological characterization of proteins purified by gabarap affinity column indicates that native gaba(a) receptors interact with gabarap. ...	2002	11948245
selective association of protein kinase c with 14-3-3 zeta in neuronally differentiated pc12 cells. stimulatory and inhibitory effect of 14-3-3 zeta in vivo.	the 14-3-3 protein is a family of highly conserved acidic proteins found in a wide range of eukaryotes from yeast to mammals. 14-3-3 acts as an adapter protein and interacts with signaling molecules including protein kinase c (pkc). although 14-3-3 zeta was originally characterized as an endogenous pkc inhibitor, it was reported to activate pkc in vitro, but the in vivo regulation of pkc by 14-3-3 is still not well understood. to examine the regulation of pkc by 14-3-3 in the cell, we have gener ...	2002	11950841
analysis of nsf mutants reveals residues involved in snap binding and atpase stimulation.	n-ethylmaleimide-sensitive fusion protein (nsf) and its yeast orthologue, sec18, are cytoplasmic aaa(+) atpases required for most intracellular membrane fusion events. the primary function of nsf is thought to be the disassembly of cis-snare complexes, thus allowing trans-snare complex formation and subsequent membrane fusion. the importance of nsf/sec18 in intracellular membrane traffic in vivo is highlighted by the inhibition of neurotransmission in drosophila comatose (nsf) mutants and of con ...	2002	11955072
distinct snare complexes mediating membrane fusion in golgi transport based on combinatorial specificity.	syntaxin-5 (sed5) is the only syntaxin needed for transport into and across the yeast golgi, raising the question of how a single syntaxin species could mediate vesicle transport in both the anterograde and the retrograde direction within the stack. sed5 is known to combine with two light chains (bos1 and sec22) to form the t-snare needed to receive vesicles from the endoplasmic reticulum. however, the yeast golgi contains several other potential light chains with which sed5 could potentially co ...	2002	11959998
glutathione synthetase from plasmodium falciparum.	gsh is the major low-molecular-mass thiol in most organisms. the tripeptide maintains a reduced intracellular environment and protects cellular components from damaging oxidation. gsh is synthesized by the action of two atp-dependent enzymic steps, in which gamma-glutamylcysteine synthetase (gamma-gcs) catalyses the ligation of glutamate and cysteine and subsequently glutathione synthetase (gs) adds glycine to the dipeptide. recently it was shown that the synthesis of gamma-glutamylcysteine is c ...	2002	11964186
binding to cisplatin-modified dna by the saccharomyces cerevisiae hmgb protein nhp6a.	nhp6a is an abundant non-histone chromatin-associated protein in saccharomyces cerevisiae that contains a minor groove dna binding motif called the hmg box. in this report, we show that nhp6ap binds to cisplatin intrastrand cross-links on duplex dna with a 40-fold greater affinity than to unmodified dna with the same sequence. nevertheless, nhp6ap bound to cisplatinated dna readily exchanges onto unmodified dna. phenanthroline-copper footprinting and two-dimensional nmr on complexes of wild-type ...	2002	11969400
tyrosine phosphorylation regulates alpha ii spectrin cleavage by calpain.	spectrins, components of the membrane skeleton, are implicated in various cellular functions. understanding the diversity of these functions requires better characterization of the interacting domains of spectrins, such as the sh3 domain. yeast two-hybrid screening of a kidney cdna library revealed that the sh3 domain of alpha ii-spectrin binds specifically isoform a of low-molecular-weight phosphotyrosine phosphatase (lmw-ptp). the alpha ii-spectrin sh3 domain does not interact with lmw-ptp b o ...	2002	11971983
emi1 is required for cytostatic factor arrest in vertebrate eggs.	vertebrate eggs are arrested at metaphase of meiosis ii with stable cyclin b and high cyclin b/cdc2 kinase activity. the ability of the anaphase-promoting complex/cyclosome (apc), an e3 ubiquitin ligase, to trigger cyclin b destruction and metaphase exit is blocked in eggs by the activity of cytostatic factor (csf) (reviewed in ref. 1). csf was defined as an activity in mature oocytes that caused mitotic arrest when injected into dividing embryos. fertilization causes a transient increase in cyt ...	2002	11976684
probing electrostatic interactions in cytochrome c using site-directed chemical modification.	this communication reports the generation of an electrostatic probe using chemical modification of methionine side chains. the alkylation of methionine by iodoacetamide was achieved in a set of saccharomyces cerevisiae iso-1-cytochrome c mutants, introducing the nontitratable, nondelocalized positive charge of a carboxyamidomethylmethionine sulfonium (camms) ion at five surface and one buried site in the protein. changes in redox potential and its variation with temperature were used to calculat ...	2002	11989715
ligand-selective potentiation of rat mineralocorticoid receptor activation function 1 by a cbp-containing histone acetyltransferase complex.	the rat mineralocorticoid receptor (mr) has two activation functions in distinct regions of the a/b domain, designated activation function 1a (af-1a; amino acids 1 to 169) and af-1b (amino acids 451 to 600). since the p160 family protein tif2, a known component of the af-2 coactivator complex, potentiates the transactivation function of af-1b but not that of af-1a, it is likely that some other, novel protein complex interacts with the af-1a region. therefore, we attempted to identify such coacti ...	2002	11997506
confirmation of uterotrophic activity of 3-(4-methylbenzylidine)camphor in the immature rat.	in this study we found that the ultraviolet sunscreen component 3-(4-methylbenzylidine)camphor (4mbc) is uterotrophic in immature rats when administered by either subcutaneous injection or oral gavage. these data confirm earlier reports of uterotrophic activity for this agent when administered to immature rats in the diet or by whole-body immersion; however, they are in contrast to negative unpublished immature rat uterotrophic assay results. data also indicate that 4mbc binds to isolated rat ut ...	2002	12003759
neuronal calcium sensor-1 binds to regulated secretory organelles and functions in basal and stimulated exocytosis in pc12 cells.	neuronal calcium sensor-1 (ncs-1) and its non-mammalian homologue, frequenin, have been implicated in a spectrum of cellular processes, including regulation of stimulated exocytosis of synaptic vesicles and secretory granules (sgs) in neurons and neuroendocrine cells and regulation of phosphatidylinositol 4-kinase beta activity in yeast. however, apart from these intriguing putative functions, ncs-1 and frequenin are relatively poorly understood. here, the distribution, dynamics and function of ...	2002	12006624
[cloning and analysis of a novel gene encoding n-terminal acetyltransferase subunit].	n-terminal acetylation is the most common modification in eukaryotic proteins, affecting stability and activity of proteins. nata is one of the n-terminal acetytransferases in yeast. it is composed of two subunits, nat1 and ard1. defect in one of them leads to loss of activity of nata. null mutant of nat1 in yeast exhibits a variety of phenotypes, including depression of a silent mating type locus (hml), failing to enter g(0) in poor nutrient situations and chromosomes instability. based on homo ...	2002	12019451
stereochemical features of the hydrolysis of 9,10-epoxystearic acid catalysed by plant and mammalian epoxide hydrolases.	cis-9,10-epoxystearic acid was used as a tool to probe the active sites of epoxide hydrolases (ehs) of mammalian and plant origin. we have compared the stereochemical features of the hydrolysis of this substrate catalysed by soluble and membrane-bound rat liver ehs, by soluble eh (purified to apparent homogeneity) obtained from maize seedlings or celeriac roots, and by recombinant soybean eh expressed in yeast. plant ehs were found to differ in their enantioselectivity, i.e. their ability to dis ...	2002	12020347
a krüppel-associated box-zinc finger protein, nt2, represses cell-type-specific promoter activity of the alpha 2(xi) collagen gene.	type xi collagen is composed of three chains, alpha 1(xi), alpha 2(xi), and alpha 3(xi), and plays a critical role in the formation of cartilage collagen fibrils and in skeletal morphogenesis. it was previously reported that the -530-bp promoter segment of the alpha 2(xi) collagen gene (col11a2) was sufficient for cartilage-specific expression and that a 24-bp sequence from this segment was able to switch promoter activity from neural tissues to cartilage in transgenic mice when this sequence wa ...	2002	12024037
cdp840. a prototype of a novel class of orally active anti-inflammatory phosphodiesterase 4 inhibitors.	the discovery, synthesis and biological activity of a series of triarylethane phosphodiesterase 4 inhibitors is described. structure-activity relationship studies are presented for cdp840 (29), a potent, chiral, selective inhibitor of pde 4 (ic(50) 4nm). cdp840 is non-emetic in the ferret at 30mgkg(-1) (po), active in models of inflammation and reverses ozone-induced bronchial hyperreactivity in the guinea pig.	2002	12031318
the identification and characterization of a noncontinuous calmodulin-binding site in noninactivating voltage-dependent kcnq potassium channels.	we show here that in a yeast two-hybrid assay calmodulin (cam) interacts with the intracellular c-terminal region of several members of the kcnq family of potassium channels. cam co-immunoprecipitates with kcnq2, kcnq3, or kcnq5 subunits better in the absence than in the presence of ca2+. moreover, in two-hybrid assays where it is possible to detect interactions with apo-cam but not with ca2+-bound calmodulin, we localized the cam-binding site to a region that is predicted to contain two alpha-h ...	2002	12032157
genomic organization, transcript variants and comparative analysis of the human nucleoporin 155 (nup155) gene.	nucleoporin 155 (nup155) is a major component of the nuclear pore complex (npc) involved in cellular nucleo-cytoplasmic transport. we have acquired the complete sequence and interpreted the genomic organization of the nup155 orthologos from human (homo sapiens) and pufferfish (fugu rubripes), which are approximately 80 and 8 kb in length, respectively. the human gene is ubiquitously expressed in many tissues analyzed and has two major transcript variants, resulted from an alternative usage of th ...	2002	12034489
synergistic activation of the rat laminin gamma1 chain promoter by the gut-enriched kruppel-like factor (gklf/klf4) and sp1.	laminin is a multifunctional heterotrimeric protein present in extracellular matrix where it regulates processes that compose tissue architecture including cell differentiation. laminin gamma1 is the most widely expressed laminin chain and its absence causes early lethality in mouse embryos. laminin gamma1 chain gene (lamc1) promoter contains several gc/gt-rich motifs including the bcn-1 element. using the bcn-1 element as a bait in the yeast one-hybrid screen, we cloned the gut-enriched kruppel ...	2002	12034813
ordered recruitment of histone acetyltransferases and the trap/mediator complex to thyroid hormone-responsive promoters in vivo.	transcriptional coactivators implicated in gene activation by the thyroid hormone receptor (tr) include members of the p160/steroid receptor coactivator (src) family of proteins, p300, and the multisubunit tr-associated protein (trap)/mediator complex. we investigated the temporal recruitment of these cofactors to mammalian thyroid hormone (t3)-responsive promoters in vivo. we show that upon t3 treatment, tr recruits all three types of coactivators to specific promoters in at least two sequentia ...	2002	12034878
interaction of sap97 with minus-end-directed actin motor myosin vi. implications for ampa receptor trafficking.	sap97 is a modular protein composed of three pdz domains, an sh3 domain, and a guanylate kinase-like domain. it has been implicated functionally in the assembly and structural stability of synaptic junctions as well as in the trafficking, recruitment, and localization of specific ion channels and neurotransmitter receptors. the n terminus of sap97 (s97n) has been shown to play a key role in the selection of binding partners and the localization of sap97 at adhesion sites, as well as the clusteri ...	2002	12050163
probing the membrane topology of a subunit of the mitochondrial protein translocase, tim44, with biotin maleimide.	tim44 is an essential component of the translocase of the inner mitochondrial membrane (tim) complex that mediates transport of nuclear encoded mitochondrial precursors across the inner membrane. here, we have investigated the topology of tim44 by probing mitochondria with membrane impermeable 3-(n-maleimidopropionyl)biocytin (mpb) followed by the specific immunoprecipitation of modified proteins. our data indicate that a single cysteine residue, cys-369, located in the c-terminal domain of the ...	2002	12054602
protein import and processing reconstituted with isolated rat liver mitochondria and recombinant mitochondrial processing peptidase.	most mitochondrial proteins are synthesized in the cytoplasm as larger precursors carrying n-terminal matrix-targeting presequences, and are subsequently transported to the mitochondria. the presequence mediates the interaction between the precursor polypeptide and components of the mitochondrial protein import machinery, a complex apparatus that is responsible for translocation of the precursor across the two mitochondrial membranes. once the precursor has reached the mitochondrial matrix, the ...	2002	12054920
rat zinc-fingers and homeoboxes 1 (zhx1), a nuclear factor-ya-interacting nuclear protein, forms a homodimer.	zinc-fingers and homeoboxes 1 (zhx1) is a protein which interacts with the activation domain of the a subunit of nuclear factor-y. to analyze the physiological role(s) of zhx1, we searched zhx1-interacting protein(s) using a yeast two-hybrid system. the rat counterpart of zhx1 cdnas was cloned from an ovarian granulosa cell complementary dna (cdna) library, indicating that zhx1 is able to form a homodimer. an analysis of the nucleotide sequence and its deduced amino acid sequence show that rat z ...	2002	12062805
implication of alpha4 phosphoprotein and the rapamycin-sensitive mammalian target-of-rapamycin pathway in prolactin receptor signalling.	a prolactin (prl)-responsive 3'-end cdna encoding rat alpha4 phosphoprotein was previously isolated from a rat lymphoma cdna library. rat alpha4 is a homologue of yeast tap42 and is a component of the mammalian target-of-rapamycin (mtor) signalling pathway that stimulates translation initiation and g1 progression in response to nutrients and growth factors. in the present study, the full-length rat alpha4 cdna was obtained by 5'-race and the 1023 bp open reading frame predicted a 340 amino acid ...	2002	12065239
brain-derived neurotrophic factor promotes interaction of the nck2 adaptor protein with the trkb tyrosine kinase receptor.	brain-derived neurotrophic factor (bdnf) binds to and activates the trkb tyrosine kinase receptor to regulate cell differentiation, survival, and neural plasticity in the nervous system. however, the identities of the downstream signaling proteins involved in this process remain unclear. using a yeast two-hybrid screen with the intracellular domain (icd-trkb) of the trkb bdnf receptor, we identified the nck2 adaptor protein as a novel interaction partner of the active form of trkb. additionally, ...	2002	12074588
ras family therapy: rab, rho and ral talk to the exocyst.		2002	12074877
masses of phosphorylated neurofilaments are associated with abnormal golgi apparatus of anterior horn neurons of beta, beta'-iminodipropionitrile-intoxicated rats.	the golgi apparatus (ga) of anterior horn neurons of rats chronically intoxicated with beta,beta'-iminodipropionitrile (idpn) in drinking water was examined with an organelle-specific antibody. the neuropile of the anterior horns contained the typical axonal spheroids associated with idpn toxicity while the perikarya of approximately one-third of the neurons contained phosphorylated neurofilaments, which are not found in the perikarya of control rat neurons. by serial or double immunostaining wi ...	2002	12075937
characterization of saccharomyces cerevisiae acyl-protein thioesterase 1, the enzyme responsible for g protein alpha subunit deacylation in vivo.	thioacylation is a reversible lipid modification of proteins that plays a role in the regulation of signal transduction. acyl-protein thioesterase 1 (apt1) was identified as an enzyme capable of deacylating some thioacylated proteins in vitro. saccharomyces cerevisiae open reading frame ylr118c encodes an enzyme homologous to rattus norvegicus apt1. we demonstrate that the catalytic activity of the protein encoded by the yeast open reading frame is similar to that of rat apt1, and we designate t ...	2002	12080046
a novel natural product compound enhances camp-regulated chloride conductance of cells expressing cftr[delta]f508.	cystic fibrosis (cf) results from mutations in the cystic fibrosis transmembrane conductance regulator (cftr) gene, which encodes a chloride channel localized at the plasma membrane of diverse epithelia. the most common mutation leading to cf, delta f508, occurs in the first nucleotide-binding domain (nbd1) of cftr. the delta f508 mutation disrupts protein processing, leading to a decreased level of mutant channels at the plasma membrane and reduced transepithelial chloride permeability. partial ...	2002	12080183
ptprj is a candidate for the mouse colon-cancer susceptibility locus scc1 and is frequently deleted in human cancers.	only a small proportion of cancers result from familial cancer syndromes with mendelian inheritance. nonfamilial, 'sporadic' cancers, which represent most cancer cases, also have a significant hereditary component, but the genes involved have low penetrance and are extremely difficult to detect. therefore, mapping and cloning of quantitative trait loci (qtls) for cancer susceptibility in animals could help identify homologous genes in humans. several cancer-susceptibility qtls have been mapped i ...	2002	12089527
coordinate regulation of udp-glucuronosyltransferase ugt1a6 induction by 3-methylcholanthrene and multidrug resistance protein mrp2 expression by dexamethasone in primary rat hepatocytes.	concentration-dependent regulation of 3-methylcholanthrene (mc) inducibility of udp-glucuronosyltransferase ugt1a6 by the synthetic glucocorticoid, dexamethasone (dex) was studied. treatment of cultured rat hepatocytes with mc, 0.1, 1, and 10 microm dex, and mc combined with dex, resulted in different induction patterns measured in the intact cells compared to that observed in the microsomes prepared from the same cells. dex treatment in various concentrations caused a concentration-dependent in ...	2002	12110372
mutations in the nucleotide binding domain 1 signature motif region rescue processing and functional defects of cystic fibrosis transmembrane conductance regulator delta f508.	the gene encoding the cystic fibrosis transmembrane conductance regulator (cftr), an atp binding cassette (abc) transporter that functions as a phosphorylation- and nucleotide-regulated chloride channel, is mutated in cystic fibrosis (cf) patients. deletion of a phenylalanine at amino acid position 508 (deltaf508) in the first nucleotide binding domain (nbd1) is the most prevalent cf-causing mutation and results in defective protein processing and reduced cftr function, leading to chloride imper ...	2002	12110684
dehydroepiandrosterone (dhea) metabolism in saccharomyces cerevisiae expressing mammalian steroid hydroxylase cyp7b: ayr1p and fox2p display 17beta-hydroxysteroid dehydrogenase activity.	we have engineered recombinant yeast to perform stereospecific hydroxylation of dehydroepiandrosterone (dhea). this mammalian pro-hormone promotes brain and immune function; hydroxylation at the 7alpha position by p450 cyp7b is the major pathway of metabolic activation. we have sought to activate dhea via yeast expression of rat cyp7b enzyme. saccharomyces cerevisiae was found to metabolize dhea by 3beta-acetylation; this was abolished by mutation at atf2. dhea was also toxic, blocking tryptopha ...	2002	12112241
mammalian mediator subunit mmed8 is an elongin bc-interacting protein that can assemble with cul2 and rbx1 to reconstitute a ubiquitin ligase.	the heterodimeric elongin bc complex has been shown to interact in vitro and in cells with a conserved bc-box motif found in an increasing number of proteins including rna polymerase ii elongation factor elongin a, suppressor of cytokine signaling (socs)-box proteins, and the von hippel-lindau tumor suppressor protein. recently, the elongin bc complex was found to function as an adaptor that links these bc-box proteins to a module composed of cullin family members cul2 or cul5 and ring-h2 finger ...	2002	12149480
postsynaptic targeting of alternative postsynaptic density-95 isoforms by distinct mechanisms.	members of the postsynaptic density-95 (psd95)/synapse-associated protein-90 (sap90) family of scaffolding proteins contain a common set of modular protein interaction motifs including pdz (postsynaptic density-95/discs large/zona occludens-1), src homology 3, and guanylate kinase domains, which regulate signaling and plasticity at excitatory synapses. we report that n-terminal alternative splicing of psd95 generates an isoform, psd95beta that contains an additional "l27" motif, which is also pr ...	2002	12151521
expression of rat multidrug resistance protein 2 (mrp2) in male and female rats during normal and pregnenolone-16alpha-carbonitrile (pcn)-induced postnatal ontogeny.	the normal maturation of biliary organic anion excretion in newborn rats can be enhanced by microsomal enzyme-inducing chemical treatment, yet the mechanism for this phenomenon is not known. multidrug resistance protein 2 (mrp2) is a biliary efflux transporter that is inducible by select microsomal enzyme-inducing chemicals. thus, the aims of this study were to compare the normal and pregnenolone-16alpha-carbonitrile (pcn)-induced postnatal ontogeny of mrp2 in male and female rats. mrp2 protein ...	2002	12167307
decreased hepatobiliary transport of methotrexate in adjuvant arthritis rats.	1. we investigated the difference in hepatobiliary transport of methotrexate in normal and adjuvant arthritis (aa) rats and substantiated the expression level of multidrug resistance-associated protein 2 (mrp2) in the liver. 2. biliary clearance of methotrexate in normal and aa rats was calculated from plasma concentrations and biliary excretion following intravenous infusion and hepatic uptake clearance was estimated from an integration plot using methotrexate concentrations in plasma and liver ...	2002	12593762
cloning and characterization of arabidopsis thaliana atnap57--a homologue of yeast pseudouridine synthase cbf5p.	rat nap57 and its yeast homologue cbf5p are pseudouridine synthases involved in rrna biogenesis, localized in the nucleolus. these proteins, together with h/aca class of snornas compose snornp particles, in which snorna guides the synthase to direct site-specific pseudouridylation of rrna. in this paper we present an arabidopsis thaliana protein that is highly homologous to cbf5p (72% identity and 85% homology) and nap57 (67% identity and 81% homology). moreover, the plant protein has conserved ...	2001	11833778
bisabosquals, novel squalene synthase inhibitors. i. taxonomy, fermentation, isolation and biological activities.	in the course of screening for yeast squalene synthase inhibitors, bisabosqual a was isolated from the culture broth of stachybotrys sp. rf-7260. the related compounds bisabosquals b, c and d were also isolated from stachybotrys ruwenzoriensis rf-6853. bisabosquals inhibited squalene synthases. ic50 values of bisabosqual a against the microsomal squalene synthases from saccharomyces cerevisiae, candida albicans, hepg2 cell and rat liver were 0.43, 0.25, 0.95 and 2.5 microg/ml, respectively. bisa ...	2001	11827030
crosstalk between myc and activating transcription factor 2 (atf2): myc prolongs the half-life and induces phosphorylation of atf2.	myc is a key regulator of cell growth, differentiation and apoptosis, and affects cell fate decisions by activating as well as by inhibiting the expression of cellular genes. myc is a member of the basic region-helix-loop-helix-leucine zipper (b-hlh-zip) class of transcription factors, which heterodimerizes with the max protein and recognizes a consensus myc binding motif. stimulation of gene expression by myc is thought to be mediated by direct binding of myc-max heterodimers to specific target ...	2001	11781825
in vitro and in vivo properties of recombinant human serum albumin from pichia pastoris purified by a method of short processing time.	recombinant human serum albumin (rhsa), secreted by a pichia pastoris expression system, was purified by a fast and efficient method, the essential feature of which is strong but reversible binding of the protein to blue sepharose. the structural characteristics, stability, and ligand-binding properties of the resulting protein were examined, and pre-clinical studies were performed.	2001	11785700
[study on anti-inflammatory, analgesic and antipyretic effects of bagmaking tea of sanyaku].	to observe anti-inflammatory, analgesic and antipyretic effects of the bagmaking tea of sanyaku in rats.	2001	11799781
classification of pdz domains.	a diverse family of pdz domains has been identified, but the rules that govern their ligand specificity are not clear. here we propose a novel classification of pdz domains based on the nature of amino acids in the two critical positions in the pdz domain fold. using these principles, we classified pdz domains present in the smart database. using yeast two-hybrid, in vitro pull-down and plasmon surface resonance assays, we demonstrated that in agreement with their position in the proposed classi ...	2001	11749973
a novel type 2c protein phosphatase from the human fungal pathogen candida albicans.		2001	11734222
interaction of dna polymerase beta with grip1 during meiosis.	dna polymerase beta (pol beta) is an essential enzyme that has been shown to localize as discrete foci to the synaptonemal complex during meiosis in the mouse. to identify proteins that associate with pol beta during meiosis, we employed the yeast two-hybrid screen. here we show that a multiple pdz domain-containing protein, the glutamate receptor interacting protein 1 (grip1), interacts specifically with pol beta. the pdz domain-containing proteins, including grip1, act as scaffolds to promote ...	2001	11734998
oxidized frying oil up-regulates hepatic acyl-coa oxidase and cytochrome p450 4 a1 genes in rats and activates pparalpha.	oxidized ldl (oxldl) and its component hydroxy fatty acids were shown to activate peroxisome proliferator-activating receptor alpha (pparalpha) and gamma (ppargamma). to test the hypothesis that lipid oxidation products in oxidized frying oil (ofo) can activate pparalpha and up-regulate its target genes, a feeding experiment and a transactivation experiment were conducted. based on a 2 x 2 factorial design, four groups of sprague-dawley male weanling rats were fed diets containing either high (2 ...	2001	11739861
expression of ccaat/enhancer binding protein family genes in monolayer and sandwich culture of hepatocytes: induction of stress-inducible gadd153.	removal of hepatocytes from their physiological environment for experimentation in vitro activates loss of liver-specific phenotype. hepatocytes cultured in a sandwich configuration reportedly maintain greater expression of certain liver-specific genes than hepatocytes in monolayer cultures. we show that sandwich culture of rat hepatocytes improves retention of expression of a liver-enriched transcription factor, c/ebpalpha (ccaat/enhancer binding protein alpha), which regulates many liver-speci ...	2001	11741281
activation of the npta element of the cyp2a3 gene by nfi-a2, a nasal mucosa-selective nuclear factor 1 isoform.	the aim of this study was to determine whether the npta element of the olfactory mucosa-predominant cyp2a3 gene can be activated by nfi-a2, a recently identified member of the nuclear factor 1 family of transcription factors. isoform-specific rna-pcr confirmed that nfi-a2 is mainly expressed in rat olfactory mucosa. a full-length nfi-a2 cdna was isolated from a cdna library of rat olfactory mucosa and was used for preparation of a construct encoding a fusion protein of nfi-a2 with the yeast gal4 ...	2001	11741324
a transcriptional switch mediated by cofactor methylation.	we describe a molecular switch based on the controlled methylation of nucleosome and the transcriptional cofactors, the creb-binding proteins (cbp)/p300. the cbp/p300 methylation site is localized to an arginine residue that is essential for stabilizing the structure of the kix domain, which mediates creb recruitment. methylation of kix by coactivator-associated arginine methyltransferase 1 (carm1) blocks creb activation by disabling the interaction between kix and the kinase inducible domain (k ...	2001	11701890
a new model of sciatic inflammatory neuritis (sin): induction of unilateral and bilateral mechanical allodynia following acute unilateral peri-sciatic immune activation in rats.	immune activation near healthy peripheral nerves may have a greater role in creating pathological pain than previously recognized. we have developed a new model of sciatic inflammatory neuritis to assess how such immune activation may influence somatosensory processing. the present series of experiments reveal that zymosan (yeast cell walls) acutely injected around the sciatic nerve of awake unrestrained rats rapidly (within 3h) produces low threshold mechanical allodynia in the absence of therm ...	2001	11731060
interaction of the alpha subunit of na,k-atpase with cofilin.	the alpha1 subunit of rat na,k-atpase, composed of 1018 amino acids, is arranged in the membrane so that the middle third of the polypeptide forms a large cytoplasmic loop bordered on both sides by multiple transmembrane segments. to identify proteins that might interact with the large cytoplasmic loop of na,k-atpase and potentially affect the function and/or the disposition of the pump in the cell, the yeast two-hybrid system was used to screen a rat skeletal muscle cdna library. several cdna c ...	2001	11139403
two rat brain staufen isoforms differentially bind rna.	in neurones, a limited number of mrnas is found in dendrites, including transcripts encoding the microtubule-associated protein 2 (map2). recently, we identified a cis-acting dendritic targeting element (dte) in map2 mrnas. here we used the yeast tri-hybrid system to identify potential trans-acting rna-binding factors of the dte. a cdna clone was isolated that encodes a member of a mammalian protein family that is highly homologous to the drosophila rna-binding protein staufen. mammalian staufen ...	2001	11145988
densin-180 forms a ternary complex with the (alpha)-subunit of ca2+/calmodulin-dependent protein kinase ii and (alpha)-actinin.	densin-180 is a transmembrane protein that is tightly associated with the postsynaptic density in cns neurons and is postulated to function as a synaptic adhesion molecule. here we report the identification of the alpha-subunit of ca(2+)/calmodulin-dependent protein kinase ii (camkii) and alpha-actinin-4 as potential binding partners for the densin-180 intracellular segment. we demonstrate by yeast two-hybrid and biochemical assays that the intracellular portion of densin-180, the alpha-subunit ...	2001	11160423
two isoforms of trimming glucosidase ii exist in mammalian tissues and cell lines but not in yeast and insect cells.	we previously cloned glucosidase ii and provided in vivo evidence for its involvement in protein folding quality control. dna-sequencing of different clones demonstrated the existence of two isoforms of glucosidase ii which differed by 66 nucleotides due to alternative splicing. the existence of two enzyme isoforms in various organs of pig and rat as well as human, bovine, rat, and mouse cell lines could be demonstrated by rt-pcr and western blotting. furthermore, the two isoforms of glucosidase ...	2001	11162524
alternative splice variants of htrp4 differentially interact with the c-terminal portion of the inositol 1,4,5-trisphosphate receptors.	the molecular basis of capacitative (or store-operated) ca2+ entry is still subject to debate. the transient receptor potential proteins have been hypothesized to be structural components of store-operated ca2+ channels and recent evidence suggests that trp3 and its closely related homolog trp6 are gated by the n-terminal region of the inositol 1,4,5-triphosphate receptors (insp3r). in this study, we report the existence of two isoforms of the human trp4 protein, referred to as alpha-htrp4 and b ...	2001	11163362
prolactin activation of irf-1 transcription involves changes in histone acetylation.	in response to prolactin (prl) signaling, transcription of the interferon regulatory factor-1 gene (irf-1) is rapidly induced during early g(1), declines in mid g(1), and rises again over the g(1)/s transition phase of the cell cycle in nb2 t cells. using chromatin immunoprecipitation assays, we show that histone h4 acetylation increases over a 1.7 kb region of the irf-1 promoter in early g(1) and again at the g(1)/s transition in response to prl stimulation. these results demonstrate a correlat ...	2001	11163802
mannosylerythritol lipid induces characteristics of neuronal differentiation in pc12 cells through an erk-related signal cascade.	rat pheochromocytoma pc12 cells undergo neuronal differentiation in response to nerve growth factor (ngf). the differentiation involves protein kinase cascades that include the kinases mek and erk, as well as activation of the transcription factors c-jun and c-fos. we show here, that exposure of pc12 cells to mannosylerythritol lipid (mel), a yeast extracellular glycolipid, enhances the activity of acetylcholinesterase and interrupts the cell cycle at the g1 phase, with resulting outgrowth of ne ...	2001	11168372
the orphan nuclear receptor nor-1 interacts with the homeobox containing protein six3.	neuron-derived orphan receptor 1 (nor-1) is a member of the ngfi-b subfamily within the nuclear receptor superfamily. in order to identify cofactors that associate with nor-1 in the fetal forebrain, we tested a yeast two-hybrid system with the nor-1 cdna fragment lacking a transactivating domain as a bait. by screening of the rat fetal brain embryonic day 17 library, a rat homologue of six3 was identified as an associated protein. we demonstrated that nor-1 interacted with six3 in yeast and in v ...	2001	11173923
association of gaba(b) receptors and members of the 14-3-3 family of signaling proteins.	two gaba(b) receptors, gaba(b)r1 and gaba(b)r2, have been cloned recently. unlike other g protein-coupled receptors, the formation of a heterodimer between gaba(b)r1 and gaba(b)r2 is required for functional expression. we have used the yeast two hybrid system to identify proteins that interact with the c-terminus of gaba(b)r1. we report a direct association between gaba(b) receptors and two members of the 14-3-3 protein family, 14-3-3eta and 14-3-3zeta. we demonstrate that the c-terminus of gaba ...	2001	11178869
sensitive bioassay for determination of fluconazole concentrations in plasma using a candida albicans mutant hypersusceptible to azoles.	the antifungal agent fluconazole (flc) is widely used in clinical practice. monitoring flc levels is useful in complicated clinical settings and in experimental infection models. a bioassay using candida pseudotropicalis, a simple and cost-effective method, is validated only for flc levels ranging from 5 to 40 mg/liter. an extension of the analytical range is needed to cover most yeast mics. a new bioassay in rpmi agar containing methylene blue was developed using c. albicans dsy1024, a mutant r ...	2001	11181345
calcium/calmodulin-dependent protein kinase ii inhibitor protein: localization of isoforms in rat brain.	a second isoform of ca2+/calmodulin-dependent-kinase ii inhibitor protein (cam-kiin) has been identified using the yeast two-hybrid screen. the 1.8kb message encodes a 78 residue cam-kiinalpha that is 65% identical in its putative open-reading frame and 95% identical in its inhibitory domain to the previously characterized cam-kiinbeta. cam-kiinalpha exhibits inhibitory properties towards recombinant mouse cam-kinase iialpha indistinguishable from cam-kiinbeta. the 27 amino acid inhibitory pepti ...	2001	11182241
role of nuclear factor-kappa b in the activation of alveolar macrophages by fungal beta-glucans.		2001	11906043
association with the nuclear matrix and interaction with groucho and runx proteins regulate the transcription repression activity of the basic helix loop helix factor hes1.	hairy/enhancer of split 1 (hes1) is a mammalian transcriptional repressor that plays crucial roles in the regulation of several developmental processes, including neuronal differentiation. the aim of this study was to elucidate the molecular mechanisms that regulate the transcription repression activity of hes1. it is shown here that hes1 associates with the nuclear matrix, the ribonucleoprotein network of the nucleus that plays important roles in transcriptional regulation. nuclear matrix bindi ...	2001	11035023
a hybrid between na+,k+-atpase and h+,k+-atpase is sensitive to palytoxin, ouabain, and sch 28080.	na(+),k(+)-atpase is inhibited by cardiac glycosides such as ouabain, and palytoxin, which do not inhibit gastric h(+),k(+)-atpase. gastric h(+),k(+)-atpase is inhibited by sch28080, which has no effect on na(+),k(+)-atpase. the goal of the current study was to identify amino acid sequences of the gastric proton-potassium pump that are involved in recognition of the pump-specific inhibitor sch 28080. a chimeric polypeptide consisting of the rat sodium pump alpha3 subunit with the peptide gln(905 ...	2001	11054424
diacylglycerol kinase zeta in hypothalamus interacts with long form leptin receptor. relation to dietary fat and body weight regulation.	leptin and its long form receptor, ob-rb, in hypothalamic nuclei play a key role in regulating energy balance. the mutation of ob-rb into one of its natural variants, ob-ra, results in severe obesity in rodents. we demonstrate here that diacylglycerol kinase zeta (dgkzeta) interacts, via its ankyrin repeats, with the cytoplasmic portion of ob-rb in yeast two-hybrid systems, in protein precipitation experiments in vitro and in vivo. it does not interact, however, with the short form, ob-ra, which ...	2001	11078732
differential regulation of growth and checkpoint control mediated by a cdc25 mitotic phosphatase from pneumocystis carinii.	pneumocystis carinii is an opportunistic fungal pathogen phylogenetically related to the fission yeast schizosaccharomyces pombe. p. carinii causes severe pneumonia in immunocompromised patients with aids and malignancies. although the life cycle of p. carinii remains poorly characterized, morphologic studies of infected lung tissue indicate that p. carinii alternates between numerous small trophic forms and fewer large cystic forms. to understand further the molecular mechanisms that regulate p ...	2001	11029470
amphiphysin 1 binds the cyclin-dependent kinase (cdk) 5 regulatory subunit p35 and is phosphorylated by cdk5 and cdc2.	amphiphysin 1 is a phosphoprotein expressed at high levels in neurons, where it participates in synaptic vesicle endocytosis and neurite outgrowth. it is a substrate for cyclin-dependent kinase (cdk) 5, a member of the cyclin-dependent protein kinase family, which has been functionally linked to neuronal migration and neurite outgrowth via its action on the actin cytoskeleton. the yeast homologue of amphiphysin, rvs167, functions in endocytosis and actin dynamics, is phosphorylated by the cdk5 h ...	2001	11113134
expression of the protein product of the pcph proto-oncogene in human tumor cell lines.	expression of the protein product of the pcph proto-oncogene in human tumor cell lines. exposure of syrian hamster embryo fibroblasts to chemical carcinogens resulted in the oncogenic activation of the pcph proto-oncogene by induction of a single base-pair deletion that generated a truncated pcph oncoprotein (mutated pcph). recently, we isolated and characterized the cdna for the human pcph proto-oncogene and determined that in humans pcph is a single-copy gene located in chromosome 14 (14q24.3) ...	2001	11121232
myomegalin is a novel protein of the golgi/centrosome that interacts with a cyclic nucleotide phosphodiesterase.	subcellular targeting of the components of the camp-dependent pathway is thought to be essential for intracellular signaling. here we have identified a novel protein, named myomegalin, that interacts with the cyclic nucleotide phosphodiesterase pde4d, thereby targeting it to particulate structures. myomegalin is a large 2,324-amino acid protein mostly composed of alpha-helical and coiled-coil structures, with domains shared with microtubule-associated proteins, and a leucine zipper identical to ...	2001	11134006
identification of zinc finger proteins bound to a silencer region in the rat glutathione transferase p gene.	the rat glutathione transferase p (gst-p) gene is strongly induced during chemical hepatocarcinogenesis, whereas mrna of this gene is rarely expressed in normal rat liver. we previously identified a silencer region in the promoter of this gene. this silencer has several dna binding sites and at least three proteins (silencer factor a, -b, and -c (sf-a, sf-b, and sf-c)) bind to these sites. we previously cloned and characterized the nuclear factor 1 (nf1) family and the ccaat/enhancer-binding pro ...	2001	11217081
characterization of a men1 ortholog from drosophila melanogaster.	multiple endocrine neoplasia type 1 (men1) is a familial cancer syndrome characterized by tumors of the parathyroid, entero-pancreatic neuroendocrine and pituitary tissues and caused by inactivating mutations in the men1 gene. menin, the 610-amino acid nuclear protein encoded by men1, binds to the transcription factor jund and can repress jund-induced transcription. we report here the identification of a men1 ortholog in drosophila melanogaster, menin1, that encodes a 763 amino acid protein shar ...	2001	11223240
identification of mouse jun dimerization protein 2 as a novel repressor of atf-2.	a mouse cdna that encodes a dna-binding protein was identified by yeast two-hybrid screening, using activating transcription factor-2 (atf-2) as the bait. the protein contained a bzip (basic amino acid-leucine zipper region) domain and its amino acid sequence was almost identical to that of rat jun dimerization protein 2 (jdp2). mouse jdp2 interacted with atf-2 both in vitro and in vivo via its bzip domain. it was encoded by a single gene and various transcripts were expressed in all tested tiss ...	2001	11231009