Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| ultrasensitive electrochemical detection for dna arrays based on silver nanoparticle aggregates. | multiplexed dna target detection is of great significance in many fields including clinical diagnostics, environmental monitoring, biothreat detection and forensics. although the emergence of dna chip technology has accelerated this process, it is still a challenge to perform ultrasensitive dna assay at low attomol concentrations so that dna detection can be directly achieved without a pcr protocol. in this work, an oligonucleotide-functionalized silver nanoparticle tag has been successfully dev ... | 2010 | 20550213 |
| the hepatitis b virus x protein disrupts innate immunity by downregulating mitochondrial antiviral signaling protein. | previous studies have shown that both hepatitis a virus and hepatitis c virus inhibit innate immunity by cleaving the mitochondrial antiviral signaling (mavs) protein, an essential component of the virus-activated signaling pathway that activates nf-kappab and ifn regulatory factor-3 to induce the production of type i ifn. for human hepatitis b virus (hbv), hepatitis b s-ag, hepatitis b e-ag, or hbv virions have been shown to suppress tlr-induced antiviral activity with reduced ifn-beta producti ... | 2010 | 20554965 |
| a gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer. | recently, our group has proposed a novel gene-regulation system responding to camp-dependent protein kinase (pka) that has been applied to living cells. in this study, human liver-specific bionanocapsules (bncs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types. bncs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo. the combination of a signal-responsive gene-delivery system with bncs led to a ... | 2010 | 20558262 |
| chronic viral infections and invasive procedures: risk of vertical transmission and current recommendations. | the risk of transmission of human immunodeficiency virus (hiv), hepatitis b (hbv) and hepatitis c virus (hcv) during invasive procedures may not be negligible, although it has been poorly assessed. the risk of hepatitis b transmission during amniocentesis seems to be low, but it may be increased in women with a positive hbeag. hcv transmission risk cannot be established because evidence is lacking. no information exists about other invasive procedures in such infections. an increased risk of ver ... | 2010 | 20558971 |
| overview of substance abuse and hepatitis c virus infection and co-infections in india. | hepatitis c virus (hcv) infection can have devastating long-term sequelae. it is very common in injecting drug users (idu) worldwide. india has a huge number of substance abusers, with an estimated 1.1 million idu. research on hcv prevalence in idu and especially other substance use is sparse. this review identified 15 such studies. some of these also studied prevalence of hepatitis b virus (hbv) and human immunodeficiency virus (hiv) infections and co-infection rates. the summary findings indic ... | 2010 | 20559752 |
| increased regulatory t cell counts in hiv-infected nonresponders to hepatitis b virus vaccine. | hepatitis b virus (hbv) coinfection is a main cause of liver-related mortality in human immunodeficiency virus (hiv)-infected subjects. unfortunately, hiv-infected subjects show a low rate of response to standard hbv vaccination (23%-56%), in contrast to rates >90% found in the general population, and the underlying causes (particularly cellular and molecular causes) are still unknown. we hypothesized that an increased frequency of regulatory t (t(reg)) cells could be involved in the low rate of ... | 2010 | 20560766 |
| [history of viral hepatitis]. | the history of viral hepatitis goes back thousands of years and is a fascinating one. when humans were first infected by such agents, a natural repetitive cycle began, with the capacity to infect billions of humans, thus decimating the population and causing sequelae in thousands of lives. | 2010 | 20563505 |
| persistent effect of ifnar-1 genetic polymorphism on the long-term pathogenesis of chronic hbv infection. | genetic polymorphism of ifnar-1 plays a large role in determining the clearance or chronicity after hepatitis b virus (hbv) exposure. however, it is not clear whether type i interferon receptor-1 (ifnar-1) variations continuously exert their effects to influence the final outcomes following hbv chronicity, including acute-on-chronic hepatitis b liver failure (aclf-hbv), chronic hepatitis b (chb), liver cirrhosis (lc), and hepatocellular carcinoma (hcc). here we report that these four potential o ... | 2010 | 20565290 |
| entry of hepatitis b virus: mechanism and new therapeutic target. | entry of hepatitis b virus (hbv) into human hepatocytes constitutes the initial step in viral infection. the study of hbv entry had long been hampered by the lack of efficient cell culture systems and small animal models. the situation was greatly improved in the last decade with the development of hbv-infectible heparg cell line and primary tupaia hepatocyte culture. armed with these new tools, marked progresses have been achieved in the elucidation of the mechanism of hbv entry. plenty of evid ... | 2010 | 20570056 |
| construction and characterization of calreticulin-hbsag fusion gene recombinant adenovirus expression vector. | to generate recombinant adenoviral vector containing calreticulin (crt)-hepatitis b surface antigen (hbsag) fusion gene for developing a safe, effective and hbsag-specific therapeutic vaccine. | 2010 | 20572313 |
| overexpressed id-1 is associated with patient prognosis and hbx expression in hepatitis b virus-related hepatocellular carcinoma. | id-1 is a member of the helix-loop-helix protein family and is involved in multiple biological processes, including development, proliferation, angiogenesis and carcinogenesis. however, the role of id-1 in hepatitis b virus (hbv)-related hepatocellular carcinoma (hcc) is still unclear. in this study, id-1 expression in 96 tumor specimens of hbv-related hcc was detected by immunohistochemical (ihc) staining. the relationship between the id-1 expression grade and patient clinicopathological featur ... | 2010 | 20574154 |
| down-regulated microrna-152 induces aberrant dna methylation in hepatitis b virus-related hepatocellular carcinoma by targeting dna methyltransferase 1. | the hepatitis b virus (hbv) x protein has been implicated as a potential trigger of the epigenetic modifications of some genes during hepatocarcinogenesis, but the underlying mechanisms remain unknown. micrornas (mirnas), which are noncoding rnas that regulate gene expression, are involved in diverse biological functions and in carcinogenesis. in this study, we investigated whether some mirnas are aberrantly expressed and involved in the regulation of the abnormal dna methylation status in hbv-r ... | 2010 | 20578129 |
| hepatitis b virus x protein blunts senescence-like growth arrest of human hepatocellular carcinoma by reducing notch1 cleavage. | one of the serious sequelae of chronic hepatitis b virus (hbv) infection is hepatocellular carcinoma (hcc). among all the proteins encoded by the hbv genome, hepatitis b virus x protein (hbx) is highly associated with the development of hcc. although notch1 signaling has been found to exert a tumor-suppressive function during hcc development, the mechanism of interaction between hbx expression and notch1 signaling needs to be explored. in this study, we report that hbx expression in hepatic and ... | 2010 | 20578140 |
| routine hepatitis b virus dna testing in patients with human immunodeficiency virus. | 2010 | 20578159 | |
| hepatitis b virus: from diagnosis to treatment. | during the next few decades, vaccination against hepatitis b virus (hbv) will dramatically change the epidemiological profile of this worldwide infection especially when heath policies encourage including hbv vaccination program for the newborns. however, it is still estimated that more than 2000millions living people have met hbv. symptomatic hepatitis with jaundice is less frequent than asymptomatic infection; however, as much as 350millions of individuals remain chronically infected by hbv. i ... | 2010 | 20580167 |
| knowledge of hcv infection among nursing students of the medical college of bitola. | healthcare workers are at risk of occupationally-acquired viral infections such as human immunodeficiency virus (hiv), hepatitis b (hbv), and hepatitis c (hcv). hcv is parenterally transmitted and has been found in every part of the world. this cross-sectional study was conducted to establish the awareness and attitude about hcv infections among 210 full-time and part-time nursing students of the medical college in bitola, macedonia from may to june 2009. for this purpose we used a self-administ ... | 2010 | 20587394 |
| factors determining successful engraftment of hepatocytes and susceptibility to hepatitis b and c virus infection in upa-scid mice. | the human liver-upa(+/+)-scid mouse is currently the best small animal model available for viral hepatitis infection studies. | 2010 | 20591528 |
| novel mechanism of antibodies to hepatitis b virus in blocking viral particle release from cells. | antibodies are thought to exert antiviral activities by blocking viral entry into cells and/or accelerating viral clearance from circulation. in particular, antibodies to hepatitis b virus (hbv) surface antigen (hbsag) confer protection, by binding circulating virus. here, we used mathematical modeling to gain information about viral dynamics during and after single or multiple infusions of a combination of two human monoclonal anti-hbs (hepex-b) antibodies in patients with chronic hepatitis b. ... | 2010 | 20593455 |
| evaluation of serum clusterin as a surveillance tool for human hepatocellular carcinoma with hepatitis b virus related cirrhosis. | hepatocellular carcinoma (hcc) is a common human cancer worldwide. the levels of serum clusterin in hcc patients and its potential diagnostic significance is not clear. we aimed to evaluate the clinical use of serum clusterin levels as a surveillance tool for hcc with hepatitis b virus (hbv) related cirrhosis. | 2010 | 20594228 |
| combining methods for speeding up multi-dimensional acquisition. sparse sampling and fast pulsing methods for unfolded proteins. | resonance assignment of intrinsically disordered proteins is made difficult by the extensive spectral overlaps. high-resolution 3d and 4d spectra are thus essential for this purpose. we have adapted the series of 3d best-experiments proposed by lescop et al. [e. lescop, p. schanda, b. brutscher, a set of best triple-resonance experiments for time-optimized protein resonance assignment, j. magn. reson. 187 (2007) 163-169] to the case of unfolded proteins. longer acquisitions in the indirect dimen ... | 2010 | 20594882 |
| hepatitis b infection and association with other sexually transmitted infections among men who have sex with men in peru. | to assess the epidemiology of hepatitis b virus (hbv) infection among men who have sex with men (msm) in peru, we evaluated the prevalence and associated risk factors for hbv serologic markers among participants of a hiv sentinel surveillance conducted in 2002-2003. the standardized prevalences for total antibodies to hepatitis b core antigen (anti-hbc) and hepatitis b surface antigen (hbsag) were 20.2% and 2.8%, respectively. individuals with human immunodeficiency virus (hiv-1) infection had s ... | 2010 | 20595501 |
| natural killer cells in viral hepatitis: facts and controversies. | hepatitis b virus (hbv) and hepatitis c virus (hcv) are major human hepatotropic pathogens responsible for a large number of chronic infections worldwide. their persistence is thought to result from inefficiencies of innate and adaptive immune responses; however, very little information is available on the former. natural killer (nk) cells are a major component of innate immunity and their activity is tightly regulated by several inhibitory and activating receptors. | 2010 | 20597961 |
| trna lys3 promoter cassettes that efficiently express rnai-activating antihepatitis b virus short hairpin rnas. | using exogenous sequences to express rna interference (rnai) activators has potential for the treatment of chronic viral infections. however, availability of a variety of suitable of promoter elements is important to optimize transcription control of silencing sequences and facilitate multitargeting. recent demonstration that trna mir genes occur naturally has prompted investigating the incorporation these trna pol iii promoters into exogenous rnai-activating cassettes. we have assessed efficacy ... | 2010 | 20599752 |
| increased expression of iaspp, regulated by hepatitis b virus x protein-mediated nf-κb activation, in hepatocellular carcinoma. | iaspp is an inhibitory member of the ankyrin-repeat-, sh3-domain- and proline-rich-region-containing protein (aspp) family; iaspp expression is up-regulated in different human tumor types. we explored the molecular mechanism increased expression of iaspp and its role in hepatocellular carcinoma (hcc). | 2010 | 20600029 |
| development of cell cultures that express hepatitis b virus to high levels and accumulate cccdna. | establishment of an infection with hepatitis b virus (hbv) requires synthesis and maintenance of a covalently closed circular dna (cccdna) form of the viral genome in the nucleus of host cells. to facilitate the investigation of the synthesis of cccdna, cell cultures were developed that express hbv to high levels. cell lines derived from hepatoma cells huh7 and hepg2 were created that express epstein-barr virus (ebv) nuclear antigen-1 and a fusion protein of the tet repressor and kox1 transcript ... | 2010 | 20600328 |
| determining rates of hepatitis c in a clozapine treated cohort. | to determine the prevalence rates of hepatitis c in patients with schizophrenia and schizoaffective disorder being treated with clozapine. | 2010 | 20605572 |
| hepatitis b virus-related polyarteritis nodosa presenting with multiple lung nodules and cavitary lesions. | the patient presented here is a 59-year-old japanese man with active chronic hepatitis b with precore and core promoter mutated virus, presenting with high fever, bloody sputum, and multiple lung nodules with excavation. surgical biopsy of the lung nodule showed necrotizing vasculitis affecting pulmonary arteries without granulomatous changes. the pulmonary manifestations of this patient resembled wegener granulomatosis. however, the pathologic findings showing nongranulomatous necrotizing vascu ... | 2010 | 20605819 |
| external quality assessment programmes for detection of hcv rna, hiv rna and hbv dna in plasma: improved proficiency of the participants observed over a 2-year period. | two external quality assessment programmes (eqaps) were run in 2008 and 2009 to evaluate the proficiency of blood centres in detecting, by nucleic acid amplification techniques (nat), the possible contamination of plasma with hepatitis c virus (hcv), human immunodeficiency virus (hiv) and hepatitis b virus (hbv). | 2010 | 20609209 |
| immunologic characterization of posthepatitis cirrhosis caused by hbv and hcv infection. | no specific treatment can reverse the liver injury in cirrhosis. this study aims to characterize immune status and correlations between cirrhosis induced by hbv and hcv. phenotypes of peripheral blood lymphocyte subsets (t, nk, regulatory t cells) and th cytokine secretion were analyzed using flow cytometry in 42 hbv-cirrhotic and 40 hcv-cirrhotic patients. cirrhotic patients had a lower proportion of cd3(+)cd8(+)t cells and nk cells, while the proportion of cd3(+)cd4(+)t cells and treg cells we ... | 2010 | 20617133 |
| hepatitis b virus surface antigen interacts with acid alpha-glucosidase and alters glycogen metabolism. | aim: hepatitis b virus (hbv) infection is highly correlated with hepatocellular carcinoma. previous studies have reported that expression of hepatitis b virus pre-s2 mutant surface antigen is related to hepatoma development. an aberrant carbohydrate metabolism is a hallmark of malignant transformation. methods: we performed yeast two-hybrid screening with hbv pre-s2-del large surface protein (pre-s2delta) by using human liver cdna library, and identified the acid alpha-glucosidase (acid alpha-gl ... | 2010 | 20618459 |
| [the virtual infected cell: a systems biology rational for antiviral drug discovery]. | infection caused by pathogens kills millions of people every year. comprehensive understanding of molecular pathogen-host interactions, i.e. the infectome, is one of the key steps towards the development of novel diagnostic, therapeutic and preventive strategies. in this quest, progress in high-throughput << omics >> technologies applied to pathogens, i.e. infectomics, opens new perspectives toward systemic understanding of perturbations induced during infection. deciphering the pathogen-host sy ... | 2010 | 20619162 |
| long-term ethanol exposure causes human liver cancer cells to become resistant to mitomycin c treatment through the inactivation of bad-mediated apoptosis. | the aim of this study was to test whether long-term ethanol consumption confers therapeutic resistance to human liver cancer patients infected with hepatitis b virus (hbv). chronic ethanol-treated cells were established by consecutively culturing a human hepatocellular carcinoma cell line, hep 3b, which contains integrated hbv sequences, for 20-40 passages with or without 10 mm ethanol (designated as e20-e40 and c20-c40, respectively). flow cytometry analysis demonstrated that a growth promoting ... | 2010 | 20623640 |
| polo-like kinase 1 activated by the hepatitis b virus x protein attenuates both the dna damage checkpoint and dna repair resulting in partial polyploidy. | hepatitis b virus x protein (px), implicated in hepatocarcinogenesis, induces dna damage because of re-replication and allows propagation of damaged dna, resulting in partial polyploidy and oncogenic transformation. the mechanism by which px allows cells with dna damage to continue proliferating is unknown. herein, we show px activates polo-like kinase 1 (plk1) in the g(2) phase, thereby attenuating the dna damage checkpoint. specifically, in the g(2) phase of px-expressing cells, the checkpoint ... | 2010 | 20624918 |
| beta-glucan oligosaccharide enhances cd8(+) t cells immune response induced by a dna vaccine encoding hepatitis b virus core antigen. | dna vaccination can induce specific cd8(+) t cell immune response, but the response level is low in large mammals and human beings. coadministration of an adjuvant can optimize protective immunity elicited by a dna vaccine. in this study, we investigated the effect of a synthetic glucohexaose (beta-glu6), an analogue of lentinan basic unit, on specific cd8(+) t cell response induced by a dna vaccine encoding hbcag (pb144) in mice. we found that beta-glu6 promoted the recruitment and maturation o ... | 2010 | 20625506 |
| [prevalence of infection for hiv, htlv, hbv and of syphilis and chlamydia in pregnant women in a tertiary health unit in the western brazilian amazon region]. | to estimate the prevalence infection of human immunodeficiency virus (hiv), human t-cell lymphotropic vírus (htlv), hepatitis b virus (hbv), chlamydia trachomatis (c. trachomatis) and syphilis in pregnant women, as well as risk factors associated with these infections, in fundação de medicina tropical do amazonas (fmtam). | 2010 | 20625686 |
| exploration of potential genomic portraits associated with intrahepatic recurrence in human hepatocellular carcinoma. | hepatocellular carcinoma (hcc) is a heterogeneous disease with recognized variability in virus infection, genetic features, and clinical outcome. to date, transcriptional profilings of hcc have been used to predict recurrence or survival/prognosis. however, there remains a challenge to identify specific genomic prints associated with hcc recurrence, which could lead to novel therapies or effective treatment. here we examine the association between biological signals and intrahepatic recurrence u ... | 2010 | 20625842 |
| management of hiv and hepatitis virus coinfection. | liver disease related to infection with hepatitis c virus (hcv) and/or hepatitis b virus (hbv) is a frequent cause of morbidity and mortality in those infected with the human immunodeficiency virus (hiv) in this era of highly active antiretroviral therapy (haart). although progress has been made in the treatment of hbv and hcv in the setting of hiv-coinfection, there is a lack of data in certain areas and several aspects of the management are unclear at this time. | 2010 | 20629586 |
| a 5' extended ifn-stimulating response element is crucial for ifn-gamma-induced tripartite motif 22 expression via interaction with ifn regulatory factor-1. | interferon-gamma is crucial for the noncytopathic clearance of hepatitis b virus. in our previous study, we demonstrated that an ifn-gamma-inducible molecule, tripartite motif (trim) 22, played an important role in antiviral immunity against hepatitis b virus. however, the molecular mechanism of trim22 induction by ifn-gamma is still unclear. in this study, we identified a novel cis-element termed 5' extended ifn-stimulating response element (5' eisre) that was crucial for ifn-gamma inducibility ... | 2010 | 20631312 |
| mir-122-induced down-regulation of ho-1 negatively affects mir-122-mediated suppression of hbv. | as the most abundant liver-specific microrna (mirna), mir-122 has been extensively studied for its role in the regulation of lipid metabolism, hepatocarcinogenesis and hepatitis c virus (hcv) replication, but little is known regarding its role in the replication of hepatitis b virus (hbv), a highly prevalent hepatotropic virus that can cause life-threatening complications. in this study we examined the effects of antisense inhibition of mir-122 and transfection of a mir-122 mimic on hbv expressi ... | 2010 | 20633528 |
| management of hepatitis b in special patient populations. | hepatitis b virus is a common cause of acute liver failure. it can be especially problematic in patients coinfected with hepatitis c, hepatitis d or human immunodeficiency virus. in addition, immunosuppression-associated hepatitis b reactivation is being increasingly recognized following chemotherapy, biologic therapy, and organ transplantation. this article highlights treatment options in these special populations. | 2010 | 20638028 |
| a microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis. | this work describes a microfluidic device integrated with multichamber polymerase chain reaction (pcr) and multichannel separation for parallel genetic analysis. the microdevice consists of three functional units: temperature control, multiple pcr (four chambers pcr), and multiple channel separation (four separation channels, each channel connected to a pcr chamber). platinum (pt)/titanium (ti) microheater was used to ensure homogeneous temperature field, and pt-chip sensor was used for temperat ... | 2010 | 20638507 |
| activated notch signaling is required for hepatitis b virus x protein to promote proliferation and survival of human hepatic cells. | hepatitis b virus x protein (hbx) is a multifunctional oncoprotein which plays a crucial role in the pathogenesis of hepatocellular carcinoma (hcc). however, the exact mechanisms remain controversial. here we show that hbx strongly stimulated cell growth, promoted cell cycle progression and inhibited apoptosis of human non-tumor hepatic cell line l02 cells. it also accelerated tumor formation of l02 cells in balb/c nude mice. furthermore, notch signaling components were upregulated in hbx-expres ... | 2010 | 20638778 |
| knockdown of hbv surface antigen gene expression by a lentiviral microrna-based system inhibits hbv replication and hcc growth. | summary. current options for the treatment of hepatitis b virus (hbv) infections, a common liver cancer risk factor, are limited. while rna interference (rnai) technologies have been shown to inhibit hbv replication, the consequent effects on hepatocellular carcinoma (hcc) cell growth are not fully understood. the aim of this study was to evaluate the effect of rnai-mediated decrease in the hbv surface antigen (hbsag) gene on hbv replication and hcc growth. a lentiviral microrna-based system exp ... | 2010 | 20642484 |
| the heparg cell line: biological properties and relevance as a tool for cell biology, drug metabolism, and virology studies. | liver progenitor cells may play an important role in carcinogenesis in vivo and represent therefore useful cellular materials for in vitro studies. the heparg cell line, which is a human bipotent progenitor cell line capable to differentiate toward two different cell phenotypes (i.e., biliary-like and hepatocyte-like cells), has been established from a liver tumor associated with chronic hepatitis c. this cell line represents a valuable alternative to ex vivo cultivated primary human hepatocytes ... | 2010 | 20645056 |
| the use of hepatocytes to investigate hdv infection: the hdv/heparg model. | worldwide, it is estimated that more than 350 million people are chronically infected with hepatitis b virus (hbv), approximately 15 million of whom are coinfected with hepatitis d virus (hdv), a satellite of hbv that uses the envelope proteins of the latter to assemble its infectious particles. for a long time after hbv discovery, research on the viral life cycle, viral entry in particular, has been hampered by the lack of practical tissue culture systems. to date, in vitro isolation and serial ... | 2010 | 20645068 |
| prevalence of hepatitis b, c, hiv and syphilis markers among refugees in bari, italy. | the aim of this study was to assess the prevalence of human immunodeficiency virus (hiv), hepatitis b virus (hbv) and hepatitis c virus (hcv) serological markers and the prevalence of vdrl positive subjects in a population of refugees of various nationalities, living in the asylum seeker centre in bari palese, southern italy. | 2010 | 20646306 |
| association of nkg2d genetic polymorphism with susceptibility to chronic hepatitis b in a han chinese population. | natural killer (nk) cells are important antiviral effectors of innate immunity because of their contribution to virus elimination. nk cell-mediated immunological reaction to hepatitis b virus (hbv) infection depends on a fine balance between inhibitory and activating receptors. the aim of the study was to investigate genetic polymorphisms in nk cell receptors (nkr)-klrd1 (cd94), klrk1 (nkg2d), klrc4 (nkg2f), and klrc1 (nkg2a)-to evaluate the association of nkr genetic polymorphisms with suscepti ... | 2010 | 20648603 |
| high prevalence of hepatitis b virus genotype e in northern madagascar indicates a west-african lineage. | the prevalence of hepatitis b virus (hbv) markers was investigated in 563 inhabitants aged 15-55 years from a sugar cane region, sirama, and from a village, mataipako, in northern madagascar. serological markers of past or present infection were significantly higher in sirama, 74% versus 45%. there was no difference in the prevalence of chronic hbsag carriers, 8.7% versus 8.5% between the two regions. sequencing the s gene in 45 strains revealed a predominance of genotype e, in 53%, followed by ... | 2010 | 20648605 |
| the prevalence of antibodies to human herpesvirus 8 and hepatitis b virus in patients in two hospitals in tanzania. | the aim of this study was to determine the seroprevalence of human herpesvirus 8 (hhv-8) and the immunization status for hepatitis b virus (hbv) infection in febrile patients in two districts of the united republic of tanzania. between february and march 2007, blood samples were collected in pemba island and tosamaganga from 336 outpatients and sent to the virology laboratory in rome (italy) for testing. hhv-8 dna and hbv-dna were amplified by two in-house molecular methods, anti-hhv-8 antibody ... | 2010 | 20648611 |
| [advances of researches in the pathogenesis of severe hepatitis b]. | severe hepatitis b is an infectious disease which has high case fatality rate and is seriously harmful to human health. its pathogenesis is complicated. in this article are reviewed the research reports on the virus and the host factors in the course of severe hepatitis b in recent years, including the advancement of researches on viral genotypes, viral mutations, immune responses and cytokines. these data are available for exploring the pathogenesis and for developing the clinical treatment of ... | 2010 | 20649047 |
| [expression of urg4 in hepatocellular carcinoma and its correlation with hbx]. | to investigate the expression of urg4 in hcc (hepatocellular carcinoma) and its correlation with hbx. | 2010 | 20491173 |
| circulating th17 cells frequency is associated with the disease progression in hbv infected patients. | th17 cells have been shown to mediate host defensive mechanisms in various infections, but their role in hbv infection in humans has not been well characterized. in this study, we analyzed the frequency and cytokines secretion of circulating th17 cells in hbv infected patients with different statuses, and also evaluated the potential association of th17 frequency with the levels of liver injury. | 2010 | 20492330 |
| course of disease and survival after onset of decompensation in hepatitis b virus-related cirrhosis. | data regarding the outcome of hepatitis b virus (hbv)-related cirrhosis after the onset of decompensation is scanty. | 2010 | 20492502 |
| development of an α-amylase reporter system for efficient screening of clones with highly expressed heterologous protein in hansenula polymorpha. | to check feasibility and effectiveness of the α-amylase reporter system, two vectors were designed and tested using hepatitis b virus surface antigen (hbsag) and homo sapiens granulocyte-macrophage colony stimulating factor 2 (hgm-csf2) as a model. by integrating the vector containing two independent cassettes into the same genome locus, high-producing clones of hbsag (or hgm-csf2) were screened using the α-amylase as a reporter. results show there was a positive correlation (correlation coeffic ... | 2010 | 20495945 |
| the cost-effectiveness of pathogen reduction technology as assessed using a multiple risk reduction model. | pathogen reduction technology (prt) for labile blood components has the potential to reduce the risk of many adverse events associated with transfusion. because of the potential broad-spectrum risk reduction capability of prt, the health economics of prt could be an important consideration in decision making for this technology. | 2010 | 20497512 |
| monoclonal antibodies specific for disease-associated point-mutants: lamin a/c r453w and r482w. | disease-linked missense mutations can alter a protein's function with fatal consequences for the affected individual. how a single amino acid substitution in a protein affects its properties, is difficult to study in the context of the cellular proteome, because mutant proteins can often not be traced in cells due to the lack of mutation-specific detection tools. antibodies, however, with their exquisite epitope specificity permit the detection of single amino acid substitutions but are not avai ... | 2010 | 20498701 |
| international society for antiviral research - 23rd international conference. | the 23rd international conference on antiviral research (icar), organized by the international society for antiviral research (isar) and held in san francisco, included topics covering new therapeutic developments in the field of antivirals. this conference report highlights selected presentations on cd4-bffi (roche holding ag), a cd4 mab-based bifunctional hiv entry inhibitor; a cldc-hbsag vaccine (juvaris biotherapeutics inc/china national biotec group) against hbv; ode-(s)-mpmpa (university o ... | 2010 | 20506054 |
| population risk of syringe reuse: estimating the probability of transmitting bloodborne disease. | in 2008, the medical officer of health at alberta health services (edmonton, canada) was notified that, in some practice settings, a syringe was used to administer medication through the side port of an intravenous circuit and then the syringe, with residual drug, was used to administer medication to other patients in the same manner. this practice has been implicated in several outbreaks of bloodborne infection in hospital and clinic settings. | 2010 | 20509761 |
| [identification and characterization of a novel hbv large protein binding protein: cdk5rap3]. | 2010 | 20510007 | |
| apobec3g directly binds hepatitis b virus core protein in cell and cell free systems. | apobec3g (a3g) is an intrinsic antiretroviral factor which can inhibit hepatitis b virus (hbv) replication. this antiviral activity mainly depends on a3g incorporation into viral particles. however, the mechanisms of a3g packaging into hbv particles have not been well characterized. in this paper, we demonstrated that a3g interacted with the hbv core protein (hbc) directly in co-transfected hepg2 cells using the fluorescence resonance energy transfer (fret) approach. in addition, we further foun ... | 2010 | 20510315 |
| a promising alternative anti-hbv agent: the targeted ribonuclease. | hbv-targeted ribonuclease (tr) is a fusion of hbv core protein (hbvc) and human eosinophil-derived neurotoxin (hedn). introduction of tr by transfection or transduction into hepg2.2.15 cells (a cell model of hbv infection) revealed that it significantly reduces serological markers of hbv replication (including hbsag, hbeag and hbv dna) in cell supernatants, suggesting that the targeted ribonuclease inhibits hbv replication. to further our understanding of the molecular mechanism of the anti-hbv ... | 2010 | 20514422 |
| scutellariae radix suppresses hepatitis b virus production in human hepatoma cells. | the traditional chinese medicine xiao-chai-hu-tang (hd-7) has been widely used to treat liver diseases in china and japan. hd-7 consists of seven different ingredients, but which one provides the therapeutic benefits is still unknown. here, we identified the "minister herb" scutellariae radix (hd-1s), but not the "king herb" bupleuri radix (hd-1b) in hd-7, as the active component that suppresses hbv gene expression and virus production in human hepatoma cells. we have found that an aqueous extra ... | 2010 | 20515825 |
| clonal expansion of normal-appearing human hepatocytes during chronic hepatitis b virus infection. | chronic hepatitis b virus (hbv) infections are associated with persistent immune killing of infected hepatocytes. hepatocytes constitute a largely self-renewing population. thus, immune killing may exert selective pressure on the population, leading it to evolve in order to survive. a gradual course of hepatocyte evolution toward an hbv-resistant state is suggested by the substantial decline in the fraction of infected hepatocytes that occurs during the course of chronic infections. consistent w ... | 2010 | 20519397 |
| massive apobec3 editing of hepatitis b viral dna in cirrhosis. | dna viruses, retroviruses and hepadnaviruses, such as hepatitis b virus (hbv), are vulnerable to genetic editing of single stranded dna by host cell apobec3 (a3) cytidine deaminases. at least three a3 genes are up regulated by interferon-alpha in human hepatocytes while ectopic expression of activation induced deaminase (aicda), an a3 paralog, has been noted in a variety of chronic inflammatory syndromes including hepatitis c virus infection. yet virtually all studies of hbv editing have confine ... | 2010 | 20523896 |
| t29c genotype polymorphism of estrogen receptor alpha is associated with initial response to interferon-alpha therapy in chronic hepatitis b patients. | virological clearance, delayed progression to cirrhosis or liver cancer, and increased survival are the long-term goals of antiviral therapy in chronic hepatitis b patients. identification of host factors correlated with therapeutic response may contribute greatly to individual treatment. this study aimed at investigating whether t29c genotype polymorphism of estrogen receptor alpha (esr1) is associated with the initial response to interferon-alpha (ifn-alpha) therapy in chronic hepatitis b pati ... | 2010 | 20525555 |
| relationship between the expression of ip-10 and ip-10 mrna in peripheral blood and hbv dna level in patients with cirrhosis. | post-hepatitic cirrhosis is regarded as common and severe form of liver damage. interferon gamma-inducible protein 10 (ip-10), a member of the non-elr (glutamic-leucine-arginine) motif cxc chemokine family, has recently been shown to recruit and activate specific subsets of leukocytes to sites of inflammation or an immune response during the development of hepatic cirrhosis. however, the effects of ip-10 and ip-10 mrna on inflammatory infiltration at local sites and in the peripheral blood of pa ... | 2010 | 20525556 |
| current risks of occupational blood-borne viral infection. | human immunodeficiency virus (hiv), hepatitis b virus (hbv), hepatitis c virus (hcv), and other viruses remain occupational risks for both surgeons and patients in the operating room environment. in the past, this concern attracted great attention, but recently, this subject has been given much less attention. | 2010 | 20528133 |
| proteomic analysis of primary duck hepatocytes infected with duck hepatitis b virus. | abstract: | 2010 | 20529248 |
| prevalence- and gender-specific immune response to opportunistic infections in hiv-infected patients in lesotho. | the objective of this study was to assess the seroprevalence of coinfecting viruses and treponema pallidum (t. pallidum) in a cohort of 205 antiretrovirally treated hiv-infected individuals (152 females and 53 males, aged: 19-71 years) in rural lesotho. furthermore agent-specific immune responses were investigated by analyzing antibody titers against herpes simplex virus type 2 (hsv-2) and against t. pallidum. | 2010 | 20531031 |
| hepatitis b virus and homo sapiens proteome-wide analysis: a profusion of viral peptide overlaps in neuron-specific human proteins. | the primary amino acid sequence of the hepatitis b virus (hbv) proteome was searched for identity spots in the human proteome by using the protein information resource database. we find that the hbv polyprotein shares sixty-five heptapeptides, one octapeptide, and one nonapeptide with the human proteins. the viral matches are disseminated among fundamental human proteins such as adhesion molecules, leukocyte differentiation antigens, enzymes, proteins associated with spermatogenesis, and transcr ... | 2010 | 20531967 |
| effects of the tp53 p.r249s mutant on proliferation and clonogenic properties in human hepatocellular carcinoma cell lines: interaction with hepatitis b virus x protein. | aflatoxin b(1) (afb(1)) is a risk factor for hepatocellular carcinoma (hcc) in many low-resource countries. although its metabolites bind at several positions in tp53, a mutation at codon 249 (agg to agt, arginine to serine, p.r249s) accounts for 90% of tp53 mutations in afb(1)-related hcc. this specificity suggests that p.r249s confers a selective advantage during hepatocarcinogenesis. using hcc cell lines, we show that p.r249s has lost the capacity to bind to p53 response elements and to trans ... | 2010 | 20538734 |
| effect of maternal hepatitis b carrier status on first-trimester markers of down syndrome. | up to 10% of women of reproductive age in our country are carriers of hepatitis b virus. this study examined whether maternal hepatitis b carrier status has any effect on markers used in first-trimester screening for down syndrome. records for 2 major taiwanese hospitals were retrospectively examined to identify women with singleton pregnancies resulting in normal live births from june 2002 through 2008. maternal hepatitis b data were used to define 3 groups: seronegative women, inactive carrier ... | 2010 | 20332164 |
| prevalence, incidence, and residual risk of major blood-borne infections among apheresis collections to the american red cross blood services, 2004 through 2008. | the number of apheresis collections increased significantly in recent years; however, data on viral marker rates among these collections are lacking. | 2010 | 20345571 |
| effect of simplification from protease inhibitors to boosted atazanavir-based regimens in real-life conditions. | atazanavir (atv) boosted with ritonavir (atv/r) is a potent, well-tolerated, once-daily protease inhibitor (pi). few data are available on this agent as a treatment simplification option for patients taking other pis. | 2010 | 20345884 |
| virologic and clinical outcomes of hepatitis b virus infection in hiv-hbv coinfected transplant recipients. | liver transplantation (lt) is the treatment of choice for end-stage liver disease, but is controversial in patients with human immunodeficiency virus (hiv) infection. using a prospective cohort of hiv-hepatitis b virus (hbv) coinfected patients transplanted between 2001-2007; outcomes including survival and hbv clinical recurrence were determined. twenty-two coinfected patients underwent lt; 45% had detectable hbv dna pre-lt and 72% were receiving anti-hbv drugs with efficacy against lamivudine- ... | 2010 | 20346065 |
| rituximab-associated infections. | after more than 10 years of use, rituximab has proven to be remarkably safe. however, accumulated evidence now suggests that under some circumstances it may significantly increase the risk of infections. this risk is difficult to quantify because of confounding factors (namely, concomitant use of immunosuppressive or chemotherapeutic agents and underlying conditions), as well as under-reporting. increased number of infections has been documented in patients treated with maintenance rituximab for ... | 2010 | 20350666 |
| prevalence of antibodies to human immunodeficiency virus (hiv), hepatitis b and hepatitis c and risk factors in prisoners in lebanon. | people admitted to correctional facilities often have a history of risky behaviours which frequently lead to transmission of blood-borne viruses, such as human immunodeficiency virus (hiv), hepatitis b virus (hbv) and hepatitis c virus (hcv). our aim was to determine the prevalence of hiv, hbv and hcv infections among prisoners in lebanon. | 2010 | 20351454 |
| hepatitis b virus x protein impairs hepatic insulin signaling through degradation of irs1 and induction of socs3. | hepatitis b virus (hbv) is a major cause of chronic liver diseases, and frequently results in hepatitis, cirrhosis, and ultimately hepatocellular carcinoma. the role of hcv in associations with insulin signaling has been elucidated. however, the pathogenesis of hbv-associated insulin signaling remains to be clearly characterized. therefore, we have attempted to determine the mechanisms underlying the hbv-associated impairment of insulin signaling. | 2010 | 20351777 |
| effect of dipterinyl calcium pentahydrate on hepatitis b virus replication in transgenic mice. | dipterinyl calcium pentahydrate (dcp) has previously been shown to inhibit mda-mb-231 human breast cancer xenographs in nude mice in a manner correlated with increases in plasma il-12 and il-4 concentrations, and decreases in plasma il-6 levels. dcp also inhibits indoleamine 2,3-dioxygenase (ido), an immuno-inhibitory enzyme, in human pbmcs (peripheral blood mononuclear cells). | 2010 | 20356392 |
| coinfection of hepatic cell lines with human immunodeficiency virus and hepatitis b virus leads to an increase in intracellular hepatitis b surface antigen. | liver-related mortality is increased in the setting of hiv-hepatitis b virus (hbv) coinfection. however, interactions between hiv and hbv to explain this observation have not been described. we hypothesized that hiv infection of hepatocytes directly affects the life cycle of hbv. we infected human hepatic cell lines expressing hbv (hep3b and ad38 cells) or not expressing hbv (huh7, hepg2, and ad43 cells) with laboratory strains of hiv (nl4-3 and ad8), as well as a vesicular stomatitis virus (vsv ... | 2010 | 20357083 |
| bax inhibitor-1 down-regulation in the progression of chronic liver diseases. | bax inhibitor-1 (bi-1) is an evolutionary conserved endoplasmic reticulum protein that, when overexpressed in mammalian cells, suppresses the apoptosis induced by bax, a pro-apoptotic member of the bcl-2 family. the aims of this study were: (1) to clarify the role of intrinsic anti- and pro-apoptotic mediators, evaluating bax and bi-1 mrna and protein expressions in liver tissues from patients with different degrees of liver damage; (2) to determine whether hcv and hbv infections modulate said e ... | 2010 | 20359348 |
| hepatitis b virus (hbv)-derived drb1*0101-restricted cd4 t-cell epitopes help in the development of hbv-specific cd8+ t cells in vivo. | we previously identified two hla-drb1*0101-restricted epitopes in hepatitis b virus (hbv) x protein (hbx) and in hbv envelope proteins (pres2). to evaluated their help in the development of cd8+ t-cell responses, mice transgenic for human class i and class ii hla molecules were immunized with hbv-t helper constructs. the pres2 epitope favored a well-balanced response with cd4+ and cd8+ t cells producing ifn-gamma, il-2 and tnf-alpha. the response was focused on cd8+ t cells with the hbx epitope. ... | 2010 | 20362206 |
| microrna-602 regulating tumor suppressive gene rassf1a is overexpressed in hepatitis b virus-infected liver and hepatocellular carcinoma. | it is important to understand the role of microrna in the transformation from chronic hbv hepatitis to hepatocellular carcinoma in hepatocarcinogenesis. relationship of microrna-602 with chronic hbv hepatitis, liver cirrhosis and hcc was investigated in this article. | 2010 | 20364114 |
| increased expression of urotensin ii and gpr14 in patients with cirrhosis and portal hypertension. | urotensin ii (uii) and its receptor (ut or gpr14) are involved in liver fibrosis and portal hypertension. nevertheless, expression of the uii/ut system in the liver of patients with portal hypertension has not been elucidated. uii and ut gene expression were quantified in liver biopsy samples from patients with hepatitis-b-virus-associated cirrhosis and portal hypertension, and from normal controls by using quantitative real-time pcr. the liver distribution of ut was determined by means of immun ... | 2010 | 20428787 |
| famciclovir: a focus on efficacy and safety. | famciclovir is the prodrug of penciclovir, a guanosine analogue that inhibits viruses of the alpha sub-family of the herpesviridae, as well as hepatitis b virus. it is indicated for management of mucocutaneous herpes simplex virus disease and acute herpes zoster, and has been investigated for management of hepatitis b virus infection. | 2010 | 20429777 |
| etiology of dna virus infections in liver transplant recipients with neonatal hepatitis. | neonatal hepatitis is a syndrome of symptoms associated with a history that includes any type of infectious, genetic, toxic, or metabolic causation. various infectious agents have been implicated in hepatic inflammation in neonates including bacterial and viral pathogens, especially dna viruses. we used molecular and antigenic methods to evaluate the role of dna viruses, such as hepatitis type b viruses (hbv), human cytomegalovirus (hcmv), herpes simplex virus (hsv), and adenovirus, in neonatal ... | 2010 | 20430185 |
| sexual behavior and hbv infection among noninjecting cocaine users (nicus). | the aim is to estimate hbv prevalence and the associated risks among noninjecting cocaine users (nicus). in 2002-2003, a total of 824 nicus from buenos aires (argentina) and montevideo (uruguay) were interviewed using a structured questionnaire. serologic tests were carried out for human immunodeficiency virus (hiv), hepatitis b (hbv), syphilis, and others. the population was divided into two serologic groups: hbv-infected and seronegative group. univariate and binary logistic model were develop ... | 2010 | 20438315 |
| hepatitis delta virus and gbv-c infection in two neighboring hepatitis b virus and hepatitis c virus-endemic villages in taiwan. | previous reports in taiwan have shown that the hepatitis b virus (hbv)- and hepatitis c virus (hcv)-endemic areas are also endemic for hepatitis d virus (hdv), gbv-c and tt virus. this study aimed to elucidate the epidemiology of hdv and gbv-c infection in two neighboring hbv- and hcv-endemic villages, to deduce the epidemiological characteristics of multiple viral infections in communities. | 2010 | 20438665 |
| lethal-7 is down-regulated by the hepatitis b virus x protein and targets signal transducer and activator of transcription 3. | the pleiotropic hepatitis b virus (hbv) x protein (hbx), associated with hepatocellular carcinoma (hcc), has been implicated in the deregulation of cellular gene expression at the transcriptional level. to date, it remains unknown if hbx regulates the expression of mirnas which play important roles in gene-regulation at the post-transcriptional and/or translational level. | 2010 | 20447714 |
| detection of ebv, hbv, hcv, hiv-1, htlv-i and -ii, and smrv in human and other primate cell lines. | the high prevalence of contaminated cell cultures suggests that viral contaminations might be distributed among cultures. we investigated more than 460 primate cell lines for epstein-barr (ebv), hepatitis b (hbv), hepatitis c (hcv), human immunodeficiency virus type 1 (hiv-1), human t-cell leukemia/lymphoma virus i and ii (htlv-i/-ii), and squirrel monkey retrovirus (smrv) infections for risk assessment. none of the cell lines were infected with hcv, hiv-1, or htlv-i/-ii. however, one cell line ... | 2010 | 20454443 |
| insulin resistance is associated with hepatocellular carcinoma in chronic hepatitis c infection. | to elucidate the role of insulin resistance (ir) and serum adiponectin level in hepatocellular carcinoma (hcc) associated with chronic hepatitis c. | 2010 | 20458764 |
| [effect of hepatitis b virus x gene on the apoptosis of hepg2 cells]. | to investigate the effect of hepatitis b virus x gene on the apoptosis in x gene-transfected hepg2 cells. | 2010 | 20369479 |
| hepatitis b virus polymerase inhibits rig-i- and toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between tbk1/ikkepsilon and ddx3. | hepatitis b virus (hbv) infection remains one of the most serious health problems worldwide. whilst studies have shown that hbv impairs interferon (ifn) production from dendritic cells in chronic hepatitis b patients, it remains unknown whether hbv inhibits ifn production in human hepatocytes. using transient transfection assays in a primary human hepatocyte cell line (ph5ch8), this study demonstrated that hbv polymerase inhibits ifn-beta promoter activity induced by newcastle disease virus, sen ... | 2010 | 20375222 |
| highly active antiretroviral therapy improved persistent lamivudine-resistant viremia in acute hepatitis b virus genotype ae infection with coinfection of human immunodeficiency virus. | a 57-year-old man developed acute hepatitis b virus (ahb), caused by hbv genotype ae. lamivudine (lam) therapy was started at 8 months after the disease onset, because the infection was persistent, but not self-limited. despite lam therapy, the hepatitis became chronic. further, virological breakthrough developed due to the emergence of lam-resistant ymdd mutants at 11 months after lam therapy. adefovir dipivoxil (adv) was combined with lam against breakthrough hepatitis at 28 months after lam t ... | 2010 | 20377825 |
| impact of human leukocyte antigen matching on hepatitis b virus recurrence after liver transplantation. | liver transplantation (lt) is an effective therapy for end-stage hepatitis b virus (hbv) infection. recurrence of hbv is one of the frequent complications. in the present study, we investigated whether human leukocyte antigen (hla) matching influences the incidence of hbv recurrence, and the time point of hbv recurrence after lt. | 2010 | 20382583 |
| impact of chronic viral diseases on semen parameters. | the aim of this study was to assess the effect of human immunodeficiency virus (hiv), hepatitis c (hcv) and b (hbv) virus infection on semen parameters. semen samples were obtained from 27 hcv, 34 hiv, 30 hbv and 41 hcv-hiv-seropositive patients and compared with those of a control population of healthy seronegative subjects. tests for detection of hiv, hcv and hbv were performed on seminal samples. the sperm concentration was significantly decreased in hcv- and hbv-seropositive males compared t ... | 2010 | 20384803 |
| cancer incidence and survival in metro manila and rizal province, philippines. | the database of two population-based cancer registries (philippine cancer society-manila cancer registry and department of health-rizal cancer registry) was used to generate age-standardized incidence rates of cancer during 1980-2002. five-year relative survival rates were obtained for incident cases from 1993 to 2002 using a period analysis method. overall incidence had increased in both males and females. among males, lung cancer was the leading cancer and reached a peak in 1988-92. colorectal ... | 2010 | 20385654 |
| the delivery of hbcag via tat-ptd enhances specific immune response and inhibits hepatitis b virus replication in transgenic mice. | recent studies have indicated that the therapeutic vaccine based on enhancement of hbv-specific cytotoxic t-lymphocyte (ctl) activity may lead to viral clearance in chronically infected individuals. it is demonstrated that protein transduction domains (ptd) from hiv-1-tat protein is able to enter cells when combined with exogenous antigens and induce specific ctl responses. we have previously testified that the expressed and purified fusion protein containing tat-ptd47-57 and hbcag could enter c ... | 2010 | 20394723 |
| assessment of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma in middle eastern patients. | alpha-fetoprotein (afp) levels for the diagnosis of hepatocellular carcinoma (hcc) may vary by geographical region and racial background. no data exists for this test in the middle eastern population. in addition, there is limited data on the impact of virological status on afp levels. | 2010 | 20397051 |
| construction of a humanized antibody to hepatitis b surface antigen by specificity-determining residues (sdr)-grafting and de-immunization. | we previously constructed a humanized antibody, hus10, by grafting the complementarity-determining regions (cdrs) of a parental murine monoclonal antibody into the homologous human antibody sequences. this process is termed cdr grafting. some residues that were thought to affect the cdr loops and stabilize the structure of the variable regions were retained in the framework region. hus10 exhibited in vivo virus-neutralizing activity, but its murine content had the potential to elicit immune resp ... | 2010 | 20399745 |