Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| geranylgeranylacetone attenuates suppression by helicobacter pylori extract of human umbilical vein epithelial cell growth. | helicobacter pylori infection delays gastric ulcer healing. angiogenesis is important for the healing of gastric ulcers. therefore, the effects of h. pylori water extract and a novel antiulcer drug, geranylgeranylacetone, on the viability of human umbilical vein epithelial cells (huvecs) were investigated. | 2004 | 15362801 |
| helicobacter pylori infection in pediatric population: epidemiology, pathophysiology, and therapy. | helicobacter pylori, one of the most commonly encountered human pathogens, has been recognized in more than half of adults and children with chronic nonspecific gastritis, and nearly all children with peptic ulcer disease demonstrate antral infection with h. pylori. a high prevalence and early colonization of h. pylori infection in childhood was described in developing countries in contrast to developed countries. the current diagnostic methods include histology, microbiological culture, urease ... | 2004 | 15371120 |
| influence of gastritis on cyclooxygenase-2 expression before and after eradication of helicobacter pylori infection. | helicobacter pylori infection causes chronic gastritis and induces cyclooxygenase (cox)-2 expression. the relationship between gastritis and cox-2 expression is not well understood, especially long after the organism has been eradicated. we designed a study to elucidate this relationship. | 2004 | 15371919 |
| cyp2c19 polymorphism is a major predictor of treatment failure in white patients by use of lansoprazole-based quadruple therapy for eradication of helicobacter pylori. | proton pump inhibitors, metabolized by the polymorphic enzyme cytochrome p450 (cyp) 2c19, are essential drugs for helicobacter pylori eradication. it was reported that patients with cyp2c19 wild type in asia had lower eradication rates. this study tests the hypothesis that cyp2c19 wild type ( wt/wt ) in white patients is also associated with a higher probability of treatment failure. | 2004 | 15371981 |
| essential domain of receptor tyrosine phosphatase beta (rptpbeta) for interaction with helicobacter pylori vacuolating cytotoxin. | helicobacter pylori produces a potent exotoxin, vaca, which causes progressive vacuolation as well as gastric injury. although vaca was able to interact with two receptor-like protein tyrosine phosphatases, rptpbeta and rptpalpha, rptpbeta was found to be responsible for gastric damage caused by vaca. to define the region of rptpbeta involved in vaca binding, we made mutants of human cdna rptpbeta-b, a short receptor form of rptpbeta. immunoprecipitation experiments to assess vaca binding to rpt ... | 2004 | 15383529 |
| oral broccoli sprouts for the treatment of helicobacter pylori infection: a preliminary report. | we sought to determine whether orally consumed broccoli sprouts could eradicate helicobacter pylori infection in infected human volunteers. helicobacter pylori-positive patients were identified by stool antigen testing or gastric biopsies. patients consumed broccoli sprouts (14, 28, or 56 g) twice daily for 7 days. we performed stool antigen testing immediately following the completion of treatment (day 8) and at day 35. urea breath testing was performed on those patients who remained negative a ... | 2004 | 15387326 |
| the diurnal profile of gastric pepsin activity is reduced with helicobacter pylori infection. | both helicobacter pylori and pepsin are proven mucosal damaging agents and implicated in the aetiology of peptic ulcer disease. historically studies of pepsin over time have proved methodologically difficult, and as a result little work has been done on the effect of h. pylori on luminal pepsin secretion. our objectives were to determine pepsin activity over 24 hr in normal human subjects and to examine luminal pepsin activity in relation to h. pylori infection. twenty-seven healthy volunteers h ... | 2004 | 15387329 |
| elevated concentrations of alpha-defensins in gastric juice of patients with helicobacter pylori infection. | defensins (alpha- and beta-defensins) are endogenous antimicrobial peptides. little is known about alpha-defensins during helicobacter pylori infection. | 2004 | 15447750 |
| solving the riddle of codon usage preferences: a test for translational selection. | translational selection is responsible for the unequal usage of synonymous codons in protein coding genes in a wide variety of organisms. it is one of the most subtle and pervasive forces of molecular evolution, yet, establishing the underlying causes for its idiosyncratic behaviour across living kingdoms has proven elusive to researchers over the past 20 years. in this study, a statistical model for measuring translational selection in any given genome is developed, and the test is applied to 1 ... | 2004 | 15448185 |
| cardiac heart disease in the era of sucrose polyester, helicobacter pylori and chlamydia pneumoniae. | recent evidence associates inflammatory mediators with coronary heart disease. elevation of acute-phase reaction (apr) proteins such as serum amyloid a, fibrinogen, crp and haptoglobin in response to helicobacter pylori (h. pylori) infection was shown to initiate gastritis and ischemic heart disease. positive chlamydia pneumoniae (c. pneumoniae) serology is associated with increased levels of inflammatory cytokines and tumor necrosis factor-alpha (tnf-alpha), which stimulates endothelial cell ac ... | 2004 | 14962637 |
| expression of nuclear factor-kappa b and target genes in gastric precancerous lesions and adenocarcinoma: association with helicobactor pylori caga (+) infection. | to examine the expression of nuclear factor kappab (nf-kappab) and its target genes in intestinal metaplasia (im), dysplasia (dys) and gastric carcinoma (gc) infected with helicobacter pylori (h pylori) and to investigate the mechanism underlying h pylori cytotoxin associated gene a (cag a) infection leading to gastric adenocarcinoma. | 2004 | 14966904 |
| identification of novel virulence-associated genes via genome analysis of hypothetical genes. | the sequencing of bacterial genomes has opened new perspectives for identification of targets for treatment of infectious diseases. we have identified a set of novel virulence-associated genes (vag genes) by comparing the genome sequences of six human pathogens that are known to cause persistent or chronic infections in humans: yersinia pestis, neisseria gonorrhoeae, helicobacter pylori, borrelia burgdorferi, streptococcus pneumoniae, and treponema pallidum. this comparison was limited to genes ... | 2004 | 14977936 |
| [are cox-2 inhibitors truly able to prevent nsaids-associated ulcer?]. | non-steroidal anti-inflammatory drugs(nsaids) have a potential to cause mucosal injury in the gastrointestinal tract. inhibitions of cyclooxygenase, one of the targets of nsaids and direct cytotoxic effects of nsaids, are reported to be involved in nsaids-related mucosal damage. it is estimated that 15-30% of patients taking nsaids develop gastroduodenal ulcers, 2% of patients have life-threatening complications. normal gastroduodenal mucosa expresses only cox-1, and it is reported that nsaids a ... | 2004 | 15038104 |
| helicobacter pylori-mediated gastritis induces local downregulation of secretory leukocyte protease inhibitor in the antrum. | helicobacter pylori-infected subjects exhibited a strong decline in antral secretory leukocyte protease inhibitor (slpi) levels compared to h. pylori-negative subjects and subjects from whom h. pylori had been eradicated (p = 0.002). this reduction was specific for the antrum, whereas slpi expression in corpus and duodenum was not affected. antral slpi levels were inversely correlated with inflammatory scores of antrum-predominant gastritis. | 2004 | 15039364 |
| the future of gastric cancer prevention. | despite advances in surgical treatment and chemotherapy, gastric cancer remains a major global health burden. the most recent estimates show that it is the fourth most common cancer and the second most common cause of cancer deaths worldwide. various etiologic factors have been linked with the disease. it is widely accepted that helicobacter pylori infection and high salt intake are positively associated with this neoplastic process. controversial associations have been found with smoking or dri ... | 2004 | 15052434 |
| genetic pathways of two types of gastric cancer. | multiple genetic and epigenetic alterations in oncogenes, tumour-suppressor genes, cell-cycle regulators, cell adhesion molecules, dna repair genes and genetic instability as well as telomerase activation are implicated in the multistep process of human stomach carcinogenesis. however, particular combinations of these alterations differ in the two histological types of gastric cancer, indicating that well-differentiated or intestinal-type and poorly differentiated or diffuse-type carcinomas have ... | 2004 | 15055305 |
| membrane channel structure of helicobacter pylori vacuolating toxin: role of multiple gxxxg motifs in cylindrical channels. | helicobacter pylori is a human pathogen responsible for severe gastric diseases such as peptic ulcers, gastric adenocarcinoma, and gastric lymphoma. vacuolating toxin (vaca) is crucial in facilitating the colonization of the gastric lining by inducing cell apoptosis and immune suppression. vaca inserts into membranes and forms a hexameric, anion-selective pore. here we present a structural model of the vaca pore that strongly resembles the structure of an unrelated anion-selective channel, mscs. ... | 2004 | 15067113 |
| determinants of helicobacter pylori seroprevalence in mexican adolescents. | helicobacter pylori infection is one of the most common human infections and is considered to play an etiologic role in several gastroduodenal diseases. in this study we determined the h. pylori seroprevalence among adolescents in morelos, mexico, and explored the association between seroprevalence and socioeconomic, dietary and lifestyle variables. | 2004 | 15068411 |
| rapid combined characterization of microorganism and host genotypes using a single technology. | genetic information is becoming increasingly important in diagnosis and prognosis of infectious diseases. in this study we investigated the possibility of using a single technology, the pyrosequencing trade mark technology (biotage ab, uppsala, sweden), to gather several kinds of important genetic information from the human pathogen helicobacter pylori, as well as from the carrier of the h. pylori infection. | 2004 | 15068415 |
| helicobacter pylori infection negatively influences pregnancy outcome in a mouse model. | helicobacter pylori infects the human stomach, causing gastritis, peptic ulcer, and gastric cancer. h. pylori infection has also been related to extra-gastric disorders. we investigated whether h. pylori infection can influence pregnancy in a murine model. | 2004 | 15068417 |
| presence of human mycoplasma dna in gastric tissue samples from korean chronic gastritis patients. | we aimed to determine whether mycoplasmas are present in korean chronic gastritis, and to understand their roles in gastric cancer tumorigenesis, because mycoplasmas resemble helicobacter pylori in terms of ammonia production and induction of inflammatory cytokines in immune and non-immune cells. the presence and identity of mycoplasmas were assessed by semi-nested pcr and sequencing, and the results were compared with pathologic data. fifty-six samples collected from korean chronic gastritis pa ... | 2004 | 15072588 |
| mucosal concentration of basic fibroblast growth factor in the healing process in human giant gastric ulcers. | basic fibroblast growth factor (bfgf) is a key factor in the healing of human and experimental peptic ulcers, but the behavior of bfgf in human giant gastric ulcer remains to be determined. we determined the bfgf content in the rim of giant ulcers (bfgf rim) and in non-ulcerated mucosa located opposite the ulcer (bfgf opposite) before and during treatment. | 2004 | 15086596 |
| evidence for the active role of a novel nuclease from helicobacter pylori in the horizontal transfer of genetic information. | helicobacter pylori is a gram-negative bacterium that colonizes the human stomach, causes gastritis, and is associated with ulcers and gastric cancer. h. pylori is naturally competent for transformation. natural genetic transformation is believed to be essential for the genetic plasticity observed in this species. while the relevance of horizontal gene transfer in h. pylori adaptiveness and antibiotic resistance is well documented, the dna transformation machinery components are barely known. no ... | 2004 | 15090498 |
| detection of helicobacter species dna by quantitative pcr in the gastrointestinal tract of healthy individuals and of patients with inflammatory bowel disease. | in many animal species different intestinal helicobacter species have been described and a few species are associated with intestinal infection. in humans, the only member of the helicobacter family which is well described in literature is helicobacter pylori. no other helicobacter-associated diseases have definitely been shown in humans. we developed a sensitive quantitative pcr to investigate whether helicobacter species dna can be detected in the human gastrointestinal tract. we tested gastri ... | 2004 | 15094170 |
| differential roles of interleukin-1beta and interleukin-8 in neutrophil transendothelial migration in patients with helicobacter pylori infection. | little information is currently available on the contribution of locally generated inflammatory and chemotactic cytokines to endothelial cell activation and subsequent neutrophil transendothelial migration in patients with helicobacter pylori (h. pylori)-associated gastritis. | 2004 | 15125462 |
| inhibition of primary human t cell proliferation by helicobacter pylori vacuolating toxin (vaca) is independent of vaca effects on il-2 secretion. | recent evidence indicates that the secreted helicobacter pylori vacuolating toxin (vaca) inhibits the activation of t cells. vaca blocks il-2 secretion in transformed t cell lines by suppressing the activation of nuclear factor of activated t cells (nfat). in this study, we investigated the effects of vaca on primary human cd4(+) t cells. vaca inhibited the proliferation of primary human t cells activated through the t cell receptor (tcr) and cd28. vaca-treated jurkat t cells secreted markedly d ... | 2004 | 15128946 |
| helicobacter pylori associated antigastric autoantibodies: role in sjögren's syndrome gastritis. | previous studies have shown that helicobacter pylori seroprevalence in sjögren's syndrome is comparable with that of the general population. however, the origin of the chronic gastropathy associated with this syndrome and the role of local autoimmunity--possibly triggered by bacterial infection--in its pathogenesis remain unclear. | 2004 | 15156903 |
| revisiting the pestilence of helicobacter pylori: insights into geographical genomics and pathogen evolution. | helicobacter pylori causes chronic gastritis and plays important roles in peptic ulcer disease, gastric carcinoma, and mucosa-associated lymphoid tissue (malt) lymphoma. it is believed that h. pylori infects over 50% of the worlds' population. however, only a small subset of infected people experience h. pylori-associated illnesses. associations with disease-specific factors remain enigmatic. the contribution of comparative genomics to our understanding of the genome organisation and diversity o ... | 2004 | 15157625 |
| [construction, expression and antigenicity of bivalent vaccine candidate of human helicobacter pylori]. | to construct a recombinant vector containing fused gene of heat shock protein a(hspa) and outer membrane protein(omp) with m(r) 18,000, from human helicobacter pylori(hp) and express the fusion protein in e.coli bl21. | 2004 | 15182624 |
| cathepsins k, l, b, x and w are differentially expressed in normal and chronically inflamed gastric mucosa. | the expression of cathepsins k, l, b, x and w was studied by quantitative rt-pcr in normal and inflamed gastric mucosa (antrum, corpus, cardia). cathepsins b, l, k and x were expressed ubiquitously. in contrast, cathepsin w was expressed at very low levels. infection by helicobacter pylori caused a significant induction of cathepsin x (p<0.008), whereas the other cathepsins were not or only locally affected by h. pylori infection or reflux disease. immunohistochemistry revealed specific expressi ... | 2004 | 15196006 |
| a confirmatory report for the close interaction of helicobacter pylori with gastric epithelial muc5ac expression. | helicobacter pylori (h. pylori) infection is associated with the development of gastritis and peptic ulcer and is presumed to be a risk factor for low-grade b-cell lymphoma and gastric cancer. h. pylori also causes critical alterations in gastric mucin structure. our aim was to determine the effect of h. pylori on muc1, muc2, and muc5ac expression. | 2004 | 15220684 |
| regression of gastric malt-lymphoma under specific therapy may be predict by endoscopic ultrasound. | mucosa-associated lymphoid tissue (malt) lymphomas represent a relatively new described class of rare lymphomas, characterized by an indolent course and favourable outcome with specific therapy. gastric malt lymphomas are associated with chronic helicobacter pylori (hp) infection. we report the case of a 67 year old man admitted for an 8-month history of epigastric pain, anorexia and progressive weight loss. he was diagnosed with low-grade primary gastric malt lymphoma by endoscopy, histopatholo ... | 2004 | 15229778 |
| gastric autoimmunity: the role of helicobacter pylori and molecular mimicry. | pathogens can induce autoreactive t cells to initiate autoimmune disease by several mechanisms. pathogen-induced inflammation results in the enhanced presentation of self antigens, which causes the expansion of the activated autoreactive t cells that are required for disease onset. alternatively, a pathogen might express antigens with epitopes that are structurally similar to epitopes of autoantigens, resulting in a mechanism of molecular mimicry. this is the case for helicobacter pylori-associa ... | 2004 | 15242679 |
| use of a novel enzyme immunoassay based on detection of circulating antigen in serum for diagnosis of helicobacter pylori infection. | recently, noninvasive diagnostic tests for helicobacter pylori infection have gained in significance. we have developed a sensitive and specific noninvasive immunoassay based on the detection of an h. pylori circulating antigen (hpca) in sera from h. pylori-infected individuals. monospecific antibody and western blot analyses were used to demonstrate the presence of the target antigen in h. pylori cell lysate and serum samples. a novel enzyme-linked immunosorbent assay (elisa) was developed for ... | 2004 | 15242956 |
| inhibition of helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by acidic polysaccharides from artemisia capillaris and panax ginseng. | helicobacter pylori specifically adheres to host cells, mainly based on carbohydrate-mediated cell-cell interactions. previously, we investigated the anti-adhesive effect of polysaccharide fractions from artemisia capillaris and panax ginseng, using hemagglutination and enzyme-linked glycosorbent assays. in the present study, each active polysaccharide fraction was further purified, resulting in a single peak (fraction f2) using gel filtration fplc, in which no protein content was detectable. us ... | 2004 | 15254854 |
| increased oxidative dna damage, inducible nitric oxide synthase, nuclear factor kappab expression and enhanced antiapoptosis-related proteins in helicobacter pylori-infected non-cardiac gastric adenocarcinoma. | several epidemiological studies have demonstrated a close association between helicobacter pylori (h pylori) infection and non-cardiac carcinoma of the stomach. h pylori infection induces active inflammation with neutrophilic infiltrations as well as production of oxygen free radicals that can cause dna damage. the dna damage induced by oxygen free radicals could have very harmful consequences, leading to gene modifications that are potentially mutagenic and/or carcinogenic. the aims of the pres ... | 2004 | 15259072 |
| induction of maturation and cytokine release of human dendritic cells by helicobacter pylori. | helicobacter pylori causes a persistent infection in the human stomach, which can result in chronic gastritis and peptic ulcer disease. despite an intensive proinflammatory response, the immune system is not able to clear the organism. however, the immune escape mechanisms of this common bacterium are not well understood. we investigated the interaction between h. pylori and human dendritic cells. dendritic cells (dcs) are potent antigen-presenting cells and important mediators between the innat ... | 2004 | 15271898 |
| chemotactic response of helicobacter pylori to human plasma and bile. | to clarify further the role of chemotaxis in helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chemoattractant for the motile bacillus. human gall-bladder bile, after dilution to 2 % (v/v) with buffer, was found to be a chemorepellent, but did not cause the motility of the bacill ... | 2004 | 15272070 |
| helicobacter pylori in children and adolescents. | there is now considerable evidence that suggests that the h. pylori organism isa human pathogen. the strong association between h. pylori and gastroduodenal disease is well documented. a number of hypotheses have been suggested for the pathogenic mechanisms of h. pylori-induced gastroduodenal disease, including the presence of bacterial virulence factors, the production of inflammatory mediators, disregulation of acid secretion, and the host immune response. at the present time, treatment with a ... | 2004 | 15272256 |
| functional adaptation of baba, the h. pylori abo blood group antigen binding adhesin. | adherence by helicobacter pylori increases the risk of gastric disease. here, we report that more than 95% of strains that bind fucosylated blood group antigen bind a, b, and o antigens (generalists), whereas 60% of adherent south american amerindian strains bind blood group o antigens best (specialists). this specialization coincides with the unique predominance of blood group o in these amerindians. strains differed about 1500-fold in binding affinities, and diversifying selection was evident ... | 2004 | 15273394 |
| herpes simplex virus 1 induced lox-1 expression in an endothelial cell line, ecv 304. | infections, such as by chlamydophilia pneumoniae, cytomegalovirus, herpes simplex virus, and helicobacter pylori, have been shown to be involved in atherogenesis. herpes simplex virus i (hsv-1) could infect vascular endothelial cells, and it has been shown that, when endothelial cells were activated with oxidized ldl (oxldl), a number of cellular events are occurred, leading to endothelial cell dysfunction. since lox-1 is a major receptor for oxldl on endothelial cells and its expression was inc ... | 2004 | 15279708 |
| diagnosis of helicobacter pylori infection in dyspeptic patients by stool antigen detection usefulness of a new monoclonal enzyme immunoassay test. | helicobacter pylori antigens can be measured in human stools with an enzyme immunoassay, which may prove to be a valuable non-invasive diagnostic tool. aim. to evaluate the usefulness of a new monoclonal enzyme immunoassay for detecting h. pylori antigens in dyspeptic patients' faeces (femtolab h. pylori, connex, martinsried, germany). | 2004 | 15285523 |
| transcription profile of helicobacter pylori in the human stomach reflects its physiology in vivo. | little is known about levels of expression of helicobacter pylori genes in the human host. we therefore developed a quantitative real-time reverse-transcriptase polymerase chain reaction (qrt-pcr) assay to measure transcript profiles of h. pylori in the human stomach. | 2004 | 15295700 |
| development of two pcr-based techniques for detecting helical and coccoid forms of helicobacter pylori. | the primary mode of transmission of helicobacter pylori, a human pathogen carried by more than half the population worldwide, is still unresolved. some epidemiological data suggest water as a possible transmission route. h. pylori in the environment transforms into a nonculturable, coccoid form, which frequently results in the failure to detect this bacterium in environmental samples by conventional culture techniques. to overcome limitations associated with culturing, molecular approaches based ... | 2004 | 15297506 |
| human polymeric iga is superior to igg and single-chain fv of the same monoclonal specificity to inhibit urease activity associated with helicobacter pylori. | helicobacter-induced gastritis is considered nowadays an epidemic, the prevalence of which is one of the highest world-wide (70%), with as much as 40% of the population in industrialized countries. helicobacter pylori (h. pylori) antigens (ag) capable to elicit a protective immune response in animal models have been identified, but these antigens have not been shown to be strongly immunogenic when administered to humans. due to their stability in the gastric environment and avidity, passive admi ... | 2004 | 15302163 |
| gastric epithelial cell cxc chemokine secretion following helicobacter pylori infection in vitro. | helicobacter pylori infection of the stomach is commonly associated with infiltration of neutrophils. gastric epithelial cells are recognized as central mediators of tissue responses to this organism. the aim of the present study was to ascertain patterns of production of three neutrophil chemoattractant chemokines following infection of gastric epithelial cells with h. pylori in vitro. | 2004 | 15304113 |
| experimental helicobacter pylori infection in humans: a multifaceted challenge. | 2004 | 15306572 | |
| challenge model for helicobacter pylori infection in human volunteers. | a reliable challenge model is needed to evaluate helicobacter pylori vaccine candidates. | 2004 | 15306577 |
| sensitivity of helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva. | helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva is thought to be one of its principal modes of transmission. among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. the sensitivity of h. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (h(2)o(2)) was investigated in buffer and in human whole saliva. the effect of lysoz ... | 2004 | 15314191 |
| nutrients released by gastric epithelial cells enhance helicobacter pylori growth. | helicobacter pylori survives and proliferates in the human gastric mucosa. in this niche, h. pylori adheres to the gastric epithelial cells near the tight junctions. in vitro, h. pylori proliferated well in tissue-culture medium near gastric epithelial cells. however, in the absence of epithelial cells, growth of h. pylori could only be established in tissue-culture medium when, prior to the experiment, it was preincubated near gastric epithelial cells. therefore, we aimed to determine whether d ... | 2004 | 15610074 |
| protective mechanism of epigallocatechin-3-gallate against helicobacter pylori-induced gastric epithelial cytotoxicity via the blockage of tlr-4 signaling. | helicobacter pylori infection leads to gastric mucosal damage by several mechanisms including the direct effect of virulence factors produced by h. pylori, propagation of inflammation, oxidative stress, dna damage, and induction of apoptosis. (-)-epigallocatechin-3-gallate (egcg), one of the green tea catechins, is known to suppress h. pylori-induced gastritis through its antioxidative and antibacterial actions. in this study, we evaluated the protective mechanism of egcg against h. pylori-induc ... | 2004 | 15610077 |
| downregulation of cxcr1 and cxcr2 expression on human neutrophils by helicobacter pylori: a new pathomechanism in h. pylori infection? | in helicobacter pylori gastritis, neutrophil activation and migration, which play central roles in the pathogenesis of the disease, are regulated by the neutrophil attractant chemokines interleukin 8 (il-8) and groalpha, whose secretion is induced by h. pylori. however, the modulation of the corresponding chemokine receptors cxcr1 and cxcr2 on human neutrophils under the influence of h. pylori has not been investigated. incubation of neutrophils with cag(+) and cag deletion h. pylori strains res ... | 2004 | 15557597 |
| role of the receptor-mediated apoptosis in helicobacter pylori in gastric epithelial cells. | two major pathways leading to apoptosis have been described. it has been shown that helicobacter pylori-mediated apoptosis is mainly effected through the mitochondrial pathway (type ii). the role of the type i pathway, including the death receptors, has been discussed controversially. therefore, we investigated the role of fas ligand (fasl) and trail in h. pylori-mediated apoptosis by overexpressing antiapoptotic proteins in the human gastric epithelial cell line ags. | 2004 | 15557763 |
| the adherent gastric mucous layer is composed of alternating layers of muc5ac and muc6 mucin proteins. | mucin-type glycoproteins are the major structural proteins in gastric mucus. stomach mucin proteins include muc5ac, synthesized by surface foveolar or pit cells, and muc6, synthesized by neck and gland cells. the aim of this study was to determine the spatial distribution of these mucin proteins within the extracellular mucous coat. double-labeling immunoflourescence/confocal microscopy was used in histologically normal surgical resection specimens. intralumenal mucin within antral glands consis ... | 2004 | 15573912 |
| spermine oxidation induced by helicobacter pylori results in apoptosis and dna damage: implications for gastric carcinogenesis. | oxidative stress is linked to carcinogenesis due to its ability to damage dna. the human gastric pathogen helicobacter pylori exerts much of its pathogenicity by inducing apoptosis and dna damage in host gastric epithelial cells. polyamines are abundant in epithelial cells, and when oxidized by the inducible spermine oxidase smo(paoh1) h(2)o(2) is generated. here, we report that h. pylori up-regulates mrna expression, promoter activity, and enzyme activity of smo(paoh1) in human gastric epitheli ... | 2004 | 15574757 |
| helicobacter-induced intestinal metaplasia in the stomach correlates with elk-1 and serum response factor induction of villin. | chronic helicobacter pylori infection results in serious sequelae, including atrophy, intestinal metaplasia, and gastric cancer. intestinal metaplasia in the stomach is defined by the presence of intestine-like cells expressing enterocyte-specific markers, such as villin. in this study, we demonstrate that villin is expressed in intestine-like cells that develop after chronic infection with h. pylori in both human stomach and in a mouse model. transfection studies were used to identify specific ... | 2004 | 15576363 |
| gastric malt lymphomas are divided into three groups based on responsiveness to helicobacter pylori eradication and detection of api2-malt1 fusion. | gastric malt lymphoma shows unique features including regression by helicobacter pylori eradication and api2-malt1 fusion. we performed a molecular and clinicopathologic study for 115 cases. all eradication-responsive cases were devoid of api2-malt1 fusion. all tumors positive for the fusion and all negative for h. pylori infection were nonresponsive to the eradication. consequently, gastric malt lymphomas were divided into three groups: eradication-responsive and fusion-negative (group a, n = 7 ... | 2004 | 15577674 |
| novel 180- and 480-base-pair insertions in african and african-american strains of helicobacter pylori. | helicobacter pylori is a genetically diverse bacterial species that chronically infects human stomachs and sometimes causes severe gastroduodenal disease. studies of polymorphic dna sequences can suggest geographic origins of individual strains. here, we describe a 180-bp insertion (ins180), which is just after the translation stop of a gene of unknown function, near the promoter of jhp0152-jhp0151 two-component signal transduction genes in strain j99, and absent from this site in strain 26695. ... | 2004 | 15583296 |
| the selective cyclooxygenase-2 inhibitor nimesulide prevents helicobacter pylori-associated gastric cancer development in a mouse model. | helicobacter pylori infection can lead to gastric cancer, and cyclooxygenase-2 (cox-2) is overexpressed in the stomach during h. pylori infection. therefore, we investigated whether nonsteroidal anti-inflammatory drugs might protect against this form of cancer. specifically, we examined the chemopreventive effect of the cox-2 inhibitor nimesulide on h. pylori-associated gastric carcinogenesis in mice. | 2004 | 15585646 |
| induction of peripheral lymph node addressin in human gastric mucosa infected by helicobacter pylori. | helicobacter pylori infects over half the world's population and is a leading cause of peptic ulcer and gastric cancer. h. pylori infection results in chronic inflammation of the gastric mucosa, and progression of chronic inflammation leads to glandular atrophy and intestinal metaplasia. however, how this chronic inflammation is induced or maintained is not well known. here, we show that chronic inflammation caused by h. pylori infection is highly correlated with de novo synthesis of peripheral ... | 2004 | 15591109 |
| gene expression in gastric biopsies from patients infected with helicobacter pylori. | helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. in this study the effects of h. pylori infection on host gene expression in the gastric antral mucosa in patients were examined. | 2004 | 15742995 |
| detection of cytomegalovirus, helicobacter pylori and chlamydia pneumoniae dna in carotid atherosclerotic plaques by the polymerase chain reaction. | cytomegalovirus (cmv), helicobacter pylori (h. pylori) and chlamydia pneumoniae (c. pneumoniae) have been associated with human atherosclerosis. the reported rates of detection within atherosclerotic lesions by pcr vary widely for all of these pathogens. we investigated their presence in carotid atherosclerotic plaques. | 2004 | 15636450 |
| upregulated akt signaling adjacent to gastric cancers: implications for screening and chemoprevention. | most gastric adenocarcinomas arise as a longterm complication of helicobacter pylori infection of the stomach, but the high prevalence of this infection limits the cost-effectiveness of antibiotic eradication as a cancer prevention strategy. here we have used phosphorylation-specific antibodies against the akt kinase consensus sequence to detect downstream substrates of this oncogenic signaling pathway in normal and malignant gastric tissues. in vitro studies confirm that phosphorylation of akt ... | 2004 | 15922857 |
| the usefulness of stable isotopes in nutrition and human health: the application of mass spectrometry and 13c-breath tests to detect helicobacter pylori infection. | the interest in helicobacter pylori has escalated in recent years. h. pylori may produce a chronic gastric infection which is usually life-long and many epidemiological studies have shown that this is the most common bacterial infection throughout the world involving 50% of the world population. thus, it is clear that the diagnosis of h. pylori infection represents at least a key step in the management of many of the patients referred to the gastroenterologist. additionally, due to the wide rang ... | 2004 | 16119720 |
| the roles of cytokines, inflammation and immunity in vascular diseases. | recent findings regarding the roles of cytokines, inflammation and immunity during the development of atherosclerosis were reviewed. especially, the relationships among pro-inflammatory cytokines such as interleukin (il)-1, il-18 and osteopontin, and anti-inflammatory cytokines such as il-1 receptor antagonist, il-10 and il-18 binding protein to inflammation and atherosclerosis were investigated and are described in detail. in addition, helicobacter pylori and c pneumoniae infections to inflamma ... | 2004 | 15644584 |
| the association of helicobacter pylori infection with coronary artery disease: fact or fiction? | helicobacter pylori (h. pylori) infection is an important cause of peptic ulcer disease and other gastrointestinal disorders. since its discovery, a number of extra-gastrointestinal diseases have been reported to be associated with h. pylori infection. recently, several epidemiological and clinical studies suggested that h. pylori infection has been associated with an increased risk of developing coronary artery disease (cad). evidence from animal studies showed that h. pylori plays an important ... | 2004 | 19861836 |
| helicobacter pylori causes gastric cancer by hijacking cell growth signaling. | extract: infection with certain strains (carrying the caga gene) of the bacterium helicobacter pylori (h. pylori) is associated with the development of gastric carcinoma. upon introduction into the gastric epithelial cells and its subsequent modification by phosphorylation (adding a phosphate group) at the tyrosine amino acid residue, caga binds specifically to a cellular oncoprotein (shp-2) and causes the oncoprotein's phosphatase activity (ridding is a phosphate group from a molecule) to misbe ... | 2004 | 20704951 |
| helicobacter pylori induces apoptosis in barrett's-derived esophageal adenocarcinoma cells. | helicobacter pylori may protect against the development of dysplasia in barrett's epithelium of patients with gastroesophageal reflux disease. the aim of this study was to determine whether h. pylori preferentially induces apoptosis in barrett's-derived cancer cells compared to normal cells. a barrett's-derived adenocarcinoma cell line (oe33) was grown. h. pylori wild-type, isogenic vaca-, caga(-), and picb-/cage- mutant strains were grown on agar plates. intact or sonicated bacteria were used t ... | 2003 | 12559187 |
| review article: nsaids, gastroprotection and cyclo-oxygenase-ii-selective inhibitors. | in patients at high risk of nsaid-associated serious upper gastrointestinal complications, gastroprotection with misoprostol or a proton pump inhibitor should be considered. only misoprostol, 800 micro g/day, has been shown to reduce serious upper gastrointestinal complications in a large clinical outcome trial. the benefit of helicobacter pylori eradication in reducing nsaid-associated gastrointestinal toxicity is controversial, and routine testing for and eradication of h. pylori in nsaid user ... | 2003 | 12562444 |
| [the prospects of vaccination against h. pylori infection]. | h. pylori causes numerous gastroduodenal diseases including malignancies. although eradication of h. pylori using antibiotics is clinically performed, it is sometimes unsuccessful because of resistant bacteria and patient non-compliance with treatment. therefore prophylactic and therapeutic vaccination against h. pylori infection is necessary for clinical use but it is still under study. h. pylori is widely believed to be transmitted in childhood. if long and chronic h. pylori infection causes g ... | 2003 | 12607329 |
| [tailor-made medicine in helicobacter pylori eradication therapy]. | pharmacokinetic profiles of omeprazole and lansoprazole were well correlated with the cyp2c19 genotype. the heterozygous extensive metabolizer was slightly different from the homozygote, but there was no statistically significant difference. the cyp2c19 genotype dependence found for lansoprazole was not obvious compared with omeprazole. as for rabeprazole, the pharmacokinetic profile was independent of the cyp2c19 genotype. cyp2c19 genotyping can provide a new strategy to choose an optimal regim ... | 2003 | 12607330 |
| the dendritic cell-specific c-type lectin dc-sign is a receptor for schistosoma mansoni egg antigens and recognizes the glycan antigen lewis x. | schistosoma mansoni soluble egg antigens (seas) are crucially involved in modulating the host immune response to infection by s. mansoni. we report that human dendritic cells bind seas through the c-type lectin dendritic cell-specific icam-3-grabbing nonintegrin (dc-sign). monoclonal antibodies against the carbohydrate antigens lewisx (lex) and galnacbeta1-4(fucalpha1-3)glcnac (ldnf) inhibit binding of dc-sign to seas, suggesting that these glycan antigens may be critically involved in binding. ... | 2003 | 12626400 |
| the rapid detection of low molecular mass proteins differentially expressed under biological stress for four helicobacter spp. using proteinchip technology. | helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. in addition h. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from h. ... | 2003 | 12627380 |
| the impact of parietal cells on helicobacter pylori tropism and host pathology: an analysis using gnotobiotic normal and transgenic mice. | helicobacter pylori infection of the human stomach is common and typically benign, although a subset of hosts develops severe pathology. infection occurs in an organ with distinct microenvironments characterized by pronounced differences in the composition of acid-producing parietal cells. in this study, we examine determinants of bacterial tropism to various gastric niches by using germ-free normal and transgenic mice with an engineered parietal cell ablation. mice were colonized for 8 weeks wi ... | 2003 | 12629225 |
| expression of heat shock protein 32 (hemoxygenase-1) in the normal and inflamed human stomach and colon: an immunohistochemical study. | heat shock protein 32 (hsp32, hemoxygenase-1) is induced by reactive oxygen metabolites (rom) and degrades heme leading to the formation of antioxidant bilirubin. increased mucosal generation of rom occurs in gastritis and inflammatory bowel disease. we aimed to assess mucosal expression of hsp32 in normal stomach and colon and to test the hypothesis that disease-related differential expression occurs in inflamed tissue. gastric body and antral mucosal biopsies were obtained from 33 patients com ... | 2003 | 15115285 |
| il-1 rn 2/2 genotype and simultaneous carriage of genotypes il-1 rn 2/2 and il-1beta-511 t/t associated with oesophagitis in helicobacter pylori-negative patients. | interleukin-1 receptor antagonist genotype 2/2 is associated with a prolonged and enhanced inflammatory response. it is suspected of being a risk factor for atrophic gastritis and gastric cancer and for some autoimmune diseases. no specific genetic risk factors for oesophagitis have been identified so far and there are no reports of il-1 polymorphism in relation to oesophageal disease. | 2003 | 14750640 |
| extracts of spice and food plants from thai traditional medicine inhibit the growth of the human carcinogen helicobacter pylori. | helicobacter pylori (hp) is a gramnegative bacterium and well recognized as being the primary etiological agent responsible for the development of gastritis, dyspepsia, peptic ulcer disease and gastric cancer. in developing countries, a high prevalence of hp infection is associated with an increased incidence of gastric cancer. thailand, however, while having a high prevalence of hp infections, has a lower than expected gastric cancer rate than other developing countries. it has been suggested t ... | 2003 | 14758718 |
| gastric stump lymphoma five years after distal gastrectomy. | we report the case of a 77-year-old man who developed low grade b cell non-hodgkin's lymphoma of the gastric stump 5 years after undergoing a distal gastrectomy for benign gastric ulcer. lymphoma occurring in the post-operative stomach would appear to be very rare, with only 14 previously recorded cases. the median period of lymphoma onset after ulcer surgery is about 20 years (range 9-43 years) and gastric remnants of lymphoma are generally diagnosed in low stage, when surgery is possible and m ... | 2003 | 12688360 |
| limited role of lipopolysaccharide lewis antigens in adherence of helicobacter pylori to the human gastric epithelium. | in vitro and in vivo studies from various groups have suggested that helicobacter pylori lipopolysaccharide (lps) lewis x (le(x)) antigens mediate bacterial adhesion. we have now reevaluated this hypothesis by studying the adherence in situ of h. pylori strain 11637 and its corresponding le(x)-negative rfbm mutant to human gastric mucosa from patients (n = 22) with various gastric pathologies. significant binding of the parent strain was observed in only 8 out of 22 sections; in four out of eigh ... | 2003 | 12704161 |
| helicobacter pylori water soluble surface proteins prime human neutrophils for enhanced production of reactive oxygen species and stimulate chemokine production. | chronic gastritis induced by helicobacter pylori is characterised by considerable neutrophil infiltration into the gastric mucosa without mucosal invasion of bacteria. bacteria have different characteristics with respect to their ability to stimulate human neutrophils to produce reactive oxygen species and chemokines. the aim of this study was to examine the effects of h pylori water extracts on the oxidative burst and chemokine production of human neutrophils. | 2003 | 12719454 |
| cytotoxic and multidrug resistance reversal activity of a vegetable, 'anastasia red', a variety of sweet pepper. | the vegetable, anastasia red, capsicum annuum l. var. angulosum mill. (solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ods) column chromatography. the biological activities of extracts and fractions were determined. these extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (hsc-2, hsg) than against normal human gin ... | 2003 | 12722138 |
| endotoxic and immunobiological activities of a chemically synthesized lipid a of helicobacter pylori strain 206-1. | a synthetic lipid a of helicobacter pylori strain 206-1 (compound hp206-1), which is similar to its natural lipid a, exhibited no or very low endotoxic activities as compared to escherichia coli-type synthetic lipid a (compound 506). furthermore, compound hp206-1 as well as its natural lipid a demonstrated no or very low mitogenic responses in murine spleen cell. on the other hand, compound hp206-1 showed a weaker but significant production of interleukin-8 in a gastric cancer cell line, mkn-1, ... | 2003 | 12727359 |
| long-term treatment with sterigmatocystin, a fungus toxin, enhances the development of intestinal metaplasia of gastric mucosa in helicobacter pylori-infected mongolian gerbils. | helicobacter pylori is a human gastric carcinogen. sterigmatocystin (st), a fungus toxin, is a risk factor of gastric cancer. cytotoxin-vacuolation toxin a (vaca) present in supernatants of h. pylori suspensions can cause gastritis and ulcer. the aim of this study was to examine the effects of h. pylori, st and vaca in mongolian gerbils. | 2003 | 12739707 |
| t helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions. | atherosclerotic lesions are infiltrated by macrophages and t lymphocytes, potentially reactive to pathogens. we studied in vivo activated t lymphocytes that infiltrate atherosclerotic plaques of helicobacter pylori-infected patients with or without anti-chlamydia pneumoniae antibodies. in all atherosclerotic lesions, t helper type 1 (th1) cells were predominant. c. pneumoniae-specific t cells were detected only in the plaques of anti-c. pneumoniae seropositive patients, whereas h. pylori-specifi ... | 2003 | 12740434 |
| strategies for the enrichment and identification of basic proteins in proteome projects. | two-dimensional gel electrophoresis (2-de) is currently the method of choice for separating complex mixtures of proteins for visual comparison in proteome analysis. this technology, however, is biased against certain classes of proteins including low abundance and hydrophobic proteins. proteins with extremely alkaline isoelectric points (pi) are often very poorly represented using 2-de technology, even when complex mixtures are separated using commercially available ph 6-11 or ph 7-10 immobilize ... | 2003 | 12748937 |
| cell adhesion-related gene expression by helicobacter pylori in gastric epithelial ags cells. | helicobacter pylori (h. pylori) infection leads to gastroduodenal inflammation, peptic ulceration and gastric carcinoma. h. pylori may induce disease-specific gene expression in gastric epithelial cells. cdna microarray for 352 cancer-related genes was used to identify the genes altered by h. pylori (caga positive) in gastric epithelial ags cells. expressions of the genes identified on the microarray and other genes closely associated with these genes were determined by reverse transcriptase-pol ... | 2003 | 12757765 |
| helicobacter pylori infection affects the expression of pcna, p53, c-erbb-2 and bcl-2 in the human gastric mucosa. | helicobacter pylori infection has been related to gastric carcinogenesis. this association is based on epidemiological data, pathological changes observed in the gastric mucosa, and chemical products from bacteria that may induce damage of dna. in the present study we examined gastric endoscopic biopsies from patients with chronic gastritis, with and without h. pylori infection, and surgical biopsies from gastric cancer patients to evaluate whether this bacteria may induce changes in the express ... | 2003 | 12760717 |
| helicobacter pylori activates toll-like receptor 4 expression in gastrointestinal epithelial cells. | helicobacter pylori activates the transcription factor nf-kappab, leading to proinflammatory cytokine production by gastric epithelial cells. however, the receptors for the initial bacterial interaction with host cells which activate downstream signaling events have not been completely defined. recently, it has been shown that microbial components activate toll-like receptors (tlrs), thereby leading to ap-1- and nf-kappab-dependent transcription and resulting in the production of proinflammatory ... | 2003 | 12761134 |
| disruption of the epithelial apical-junctional complex by helicobacter pylori caga. | helicobacter pylori translocates the protein caga into gastric epithelial cells and has been linked to peptic ulcer disease and gastric carcinoma. we show that injected caga associates with the epithelial tight-junction scaffolding protein zo-1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight-junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex. long-term caga delivery to p ... | 2003 | 12775840 |
| increased zinc finger protein zfoc1 transcripts in gastric cancer compared with normal gastric tissue. | screening of cdna arrays of the image library identified human zfoc1 as a differentially expressed cdna that was upregulated in kato iii gastric cancer cells following stimulation with the gastric pathogen helicobacter pylori. | 2003 | 12782764 |
| trailing gastrointestinal pathogenesis. | 2003 | 12795744 | |
| effect of total secondary carotenoids extracts from chlorococcum sp on helicobacter pylori-infected balb/c mice. | helicobacter pylori is a human pathogen associated with type b gastritis, peptic ulcer disease and gastric cancer. a high intake of carotenoids has been suggested to prevent development of gastric malignancies. microalgae chlorococcum sp. could accumulate secondary carotenoids under stress conditions. the aim of the present study was to investigate whether dietary cell extract of chlorococcum sp. could affect the bacterial load of h. pylori infected balb/c mice and whether it could induce modula ... | 2003 | 12810355 |
| expression of cd3 epsilon subunit in gastric parietal cells: a possible role in signal transduction? | cd3 antigen, formerly thought to be specific for t lymphocytes, has been found on gastric parietal cells in animals and humans. the common anti-cd3 antibodies recognize the epsilon subunit, which has a role in signal transduction. the aim of this study was to immunostain stomach specimens from humans and different animal species for cd3 antigen to determine if cd3 antigen is conserved across species and if cd3 antigen expression is altered in humans by use of certain drugs or the presence of gas ... | 2003 | 12812314 |
| priming and expression of immune responses in the gastric mucosa. | this review deals with the induction and expression of immune responses in the human stomach following mucosal immunisation. as prerequisites for developing a helicobacter pylori vaccine, the role of gastric inflammation, in particular inflammation induced by h. pylori infection, as well as lymphocyte homing within the common mucosal immune system, and the importance of immunisation route are discussed. | 2003 | 12814774 |
| animal models of helicobacter pylori infection and disease. | the acceptance of helicobacter pylori as a major human pathogen has necessitated the development of animal models to help elucidate the pathogenic mechanisms of this bacterium and aid in the development of improved strategies for the treatment of gastric disease. appropriate models, utilising a range of animal species, have been developed to examine factors such as the influence of host responses and bacterial factors in disease development and the success of new therapeutic regimens, including ... | 2003 | 12814775 |
| molecular mimicry in atherosclerosis: a role for heat shock proteins in immunisation. | atherosclerosis has long been recognised as having an inflammatory component, and this has a particularly important bearing on to its clinical complications as it may result in plaque instability. results of recent epidemiological studies have reinforced the potential importance of this aspect of the disease. positive associations have been reported between exposure to several specific pathogens, and future risk of coronary heart disease (chd). whilst it is possible that each individual organism ... | 2003 | 12818399 |
| time-dependent amplified pharmacokinetic and pharmacodynamic responses of rabeprazole in cytochrome p450 2c19 poor metabolizers. | to determine the pharmacokinetic and pharmacodynamic rationale for the optimum regimen of rabeprazole in the treatment of helicobacter pylori infection in patients who are cytochrome p450 (cyp) 2c19 poor metabolizers or extensive metabolizers. | 2003 | 12820812 |
| gastric transitional zones, areas where helicobacter treatment fails: results of a treatment trial using the sydney strain mouse model. | current combination therapies cure helicobacter pylori infection in 75 to 85% of cases. however, many treatment failures are not explained by antibiotic resistance. our goal was to explore treatment failures under in vivo conditions by using the h. pylori sydney strain (ss1) mouse model. mice infected with h. pylori (ss1) were treated with monotherapies or combination therapies used in human trials. bacterial levels and distribution of organisms within the stomach were assessed 24 h after treatm ... | 2003 | 12821476 |
| association between infections and signs and symptoms of 'atopic' hypersensitivity--results of a cross-sectional survey among first-year university students in germany and spain. | this study assessed the hypothesis that a decreased exposure to childhood infectious diseases is associated with signs and symptoms of 'atopic' hypersensitivity diseases. | 2003 | 12823114 |
| [chronic urticaria and infectious diseases]. | infectious diseases are often considered as a classic cause of chronic urticaria. nevertheless, laboratory investigations greatly vary from one centre to the other and the link between the infection and skin signs does not rely on hard data. the purpose of this work was a systematic analysis of the published cases of urticaria associated with infection. | 2003 | 12843809 |