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the haemolytic plaque assay in carp (cyprinus carpio).a haemolytic plaque assay for the enumeration of antibody forming cells in the carp (cyprinus carpio) is described. serum of bream (abramis brama) turned out to be a more reliable complement source than allogeneic carp serum. the addition of 3--5% bream serum to a mixture of immune lymphoid carp cells and xenogeneic erythrocytes gave optimal results. higher amounts of bream complement inhibited plaque formation. plaque formation was also suppressed when inactive or heat-inactivated bream or carp ...19806987315
[examination of some strains of the aeromonas hydrophila-punctata-group isolated from carp by resistance tests (author's transl)]. 19806993174
cloning of carp preproinsulin cdna in the bacterial plasmid pbr322.the successful cloning of recombinants between cdna from fractionated poly(a)+-rna of brockmann bodies of the carp and the plasmid pbr322 in escherichia coli chi 1776 is reported. one of the recombinant clones has been identified as a preproinsulin-cdna recombinant by the hybrid-arrest translation assay. recombination was at the psti site of pbr322; reconstitution of this site was by 3'-tailing of the vector with dgn. the transformants were screened by in situ hybridization with kinase-labeled p ...19806993287
biosynthesis of proinsulin of carp (cyprinus carpio) and characterization and cloning of mrna. 19806994550
the immune system of cyprinid fish. kinetics and temperature dependence of antibody-producing cells in carp (cyprinus carpio).after immunization of carp with sheep red blood cells, the spleen accounts for only 5% of the total number of plaque-forming cells (pfc). in addition, thymus, peripheral blood and heart contained low numbers of pfc (< 0.5, 1 and 0.5%, respectively). pronephros and mesonephros were the major antibody-forming organs (53 and 40% of total pfc, respectively). the temperature dependence of the antibody-forming cell response in spleen, pronephros and mesonephros as studied in animals kept at 12-24 degr ...19807000695
influence of nutritional status on the daily patterns of nitrogen excretion in the carp (cyprinus carpio l.) and the rainbow trout (salmo gairdneri r.).nitrogen (ammonia and/or urea) excretion in carp and rainbow trout kept under different feeding regimes was continuously monitored over 24-hr cycles. the daily nitrogen excretion patterns resulting from these feeding regimes were studied over several days after a change from one made to another. constant levels of endogenous nitrogen excretion were reached about one week after the start of a fasting period; almost one week was needed for the daily nitrogen excretion pattern to stabilize after th ...19807349509
interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate.calmodulin has been purified to homogeneity using an improved procedure that allows rapid processing of several kilograms of bovine brain. a calcium-dependent change in the electrophoretic mobility of calmodulin in the presence of sodium dodecyl sulfate (sds) has been observed. freshly prepared calmodulin or lyophilized calmodulin, stored at --80 degrees c for 1--7 months, migrates as a single band with an apparent molecular weight of 21 000 when the sample, gel and running buffer are made 0.1 m ...19807397213
size related toxicity of copper & mercury to lebistes reticulatus (peter), labeo rohita (ham.) & cyprinus carpio linn. 19807399616
influence of quaternary structure on the state of the haem in carp and human methaemoglobins studied by resonance raman scattering.the resonance raman spectra of carp and human methaemoglobin (methb) derivatives in the t and r quaternary structures were measured in the frequency regions of 1200-1700 cm-1 and 100-600 cm-1. conversion of carp and human fluoro methb's to the t structure was accompanied by a frequency shift of the raman line at 348 cm-1 to lower frequency by 5 cm-1, but all other lines were unaffected by the r-t transition. as the 348 cm-1 line was assigned to a porphyrin mode (v8) involving a bending motion of ...19807407241
binding of non-mammalian immunoglobulins to staphylococcal protein a.immunoglobulins m of the chicken (about 3% of serum igm), clawed toad (12%) and carp (13%) bind to the protein a-sepharose. a monomeric immunoglobulin with four domain heavy chains from the chicken serum is also bound (30% of bound immunoglobulins). the protein a-sepharose can be utilized for isolation of igm of the clawed toad and carp directly from the serum.19807409269
investigations on the origin of two radiation-induced compounds in irradiated meat.after irradiation with 0.5 mrad or higher doses, a radiation-induced ninhydrin-positive compounds was found in the muscle tissue of warm-blooded animals. in carp muscle another compound called x was formed. the origin of these two compounds was investigated. irradiation of various amino acids and related compounds showed that substance y is formed from the dipeptides carnosine and anserine, which are present in muscle tissue of warm-blooded animals unusually high concentrations. in carp muscle c ...19807415531
[comparative studies of the specificity of idiotypic guinea pig and carp antisera].the specificity of guinea-pig- and carp-anti-idiotypic antisera were compared by using the radioimmunoassay and the passive hemagglutination inhibition test. guinea-pigs and carps were immunized with the iga/k mouse myeloma protein s117 which has binding activity to the hapten n-acetylglucosamine. the resulting antisera were idiotypically specified by absorption and the activity was determined by the binding of the radiolabeled idiotype s117. the binding curves of guinea pig and carp anti-idioty ...19807424349
carp hemoglobin. i. precise oxygen equilibrium and analysis according to the models of adair and of monod, wyman, and changeux.precise oxygen equilibria for carp hemoglobin had been measured from about 1% to 99% saturation at 15 degrees c. the determinations were made in 2,2-bis(hydroxymethyl)-2,2',2"-nitrilotriethanol buffer and in phosphate buffer in the presence and absence of p6-inositol (ihp). at the highest cooperativity, carp hemoglobin has a maximum hill coefficient, n, of 2.3 and free energy of interaction, delta gi, of 2100 cal/site; addition of ihp decreases these values to 1.6 and 1640 cal/site, respectively ...19807430103
[hemoglobins, xxxv: the sequence of the beta a- und beta b-chains of the hemoglobins of the carp (cyprinus carpio l.)].the primary structure of the beta a- and beta b-chains from both main components of the tetrameric hemoglobins from carp is given. the sequences were found by automatic analysis in the sequenator using a hydrophilic and hydrophobic program with hydrophilic isothiocyanate. the beta-chains a and b differ in positions beta 13, beta 41, beta 122 and beta 143. in comparison to human beta-chains carp beta a- and beta b-chains show 66 and 67 residues, respectively (45%). furthermore, we found one inser ...19807450680
comparison of the pineal complex, retina and cerebrospinal fluid contacting neurons by immunocytochemical antirhodopsin reaction.the presence of rhodopsin was investigated by an indirect immunocytochemical method in the pineal complex of various vertebrates (carassius auratus, cyprinus carpio, hypophthalamichthys molitrix, lucioperca lucioperca, triturus vulgaris, bombina bombina, rana esculenta, pseudemys scripta elegans, lacerta agilis et viridis, white leghorn chickens, rat), in the retina of lebistes reticulatus, lucioperca lucioperca, rana esculenta, lacerta agilis, pseudemys scripta elegans, the chicken and the rat, ...19807456628
presence of liver-forming fraction in fish egg mrnas detected by its ability to encode albumin synthesis.poly a attached rnas were isolated from carp ovarian and mature eggs. both were equally potent in cell-free translating wheat germ system. the products were analyzed with rabbit antiserum against carp albumin and polyacrylamide gel electrophoresis. the results revealed the presence of carp albumin, a liver specific protein.19807190727
[chromosome 18 long-arm deletion. report of 1 case].authors report a ten month old girl with multiple malformations: midfacial hypoplasia, hypertelorism, carp mouth, very marked antihelix, poor muscular tone, spindle-shaped fingers with a high frequency of whirles and low levels of serum immunoglobulin a. in leukocyte culture, patient shows a deletion of the long arm of a 18 chromosome.19807212462
does rigidity in structure of muscarinic agonists and antagonists reflect drug specificity?the present work is an attempt to elucidate: (1) whether highly rigid structural analogs of acetylcholine are still capable of activating the muscarinic receptor; (2) whether such analogs, be they agonists or antagonists, discriminate among the various ach-mediated functions, thereby providing a tool for the study of a possible receptor heterogeneity; (3) whether structural rigidity is a significant factor in the kinetics of drug-receptor interaction. to this end, we investigated some properties ...19807231438
[the wormicidal substances of fresh water fishes on clonorchis sinensis: ii. preliminary research on the wormicidal substances from mucous substances of various fresh water fishes]the present work which was investigated in july 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (cyprinus carpio, parasilurus asotus, anguilla japonica, ophicephalus argus, carassius carassius; golden crusian carp, misgurnus anguillicaudatus, zacco platypus, pseudorasbora parva and carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of clonorchis sinensis. the mucous substances extracted by ether from t ...198012902746
subcellular distribution and some properties of alanine aminotransferase in striated muscles of the crayfish, trout, carp, frog, pigeon and rabbit.subcellular distribution and some physicochemical properties of alanine aminotransferase in striated muscles of the crayfish, trout, carp, frog, pigeon and rabbit were studied. it was established that: (1) alanine aminotransferase activity in all mentioned animals occurred almost entirely in the cytosolic fraction of the muscles. total activity and activity per mg protein were highest in crayfish and pigeon muscles and lowest in carp and trout muscles. (2) the ph optimum for the muscles of homoi ...197938468
molecular adaptability of carp myosin: a study of some physico-chemical properties and their comparison with those of rabbit myosin.during thermal inactivation, the addition of as low as m urea resulted in the reduction of delta g identical to barrier of the inactivation of carp myosin ca2+-atpase, whereas that of rabbit myosin remained unaffected. in the absence of urea, a four-hour incubation of carp myosin was accompanied by the release of light chains at 30 degrees c, a value 10 degrees c lower than that for rabbit myosin. electron micrographs revealed that carp myosin forms artificial thick filaments, which were uniform ...197993883
[structural and immunochemical studies of carp (cyprinus carpio l.) immunoglobulin. v. tryptic fragments of carp (cyprinus carpio l.) immunoglobulin m].carp igm, isolated from normal serum is more sensitive to trypsinization compared to a human myeloma protein igmgo. under the same conditions (treatment with trypsin at 56 degrees c for 30 min) carp igm was degraded to small, mostly dialysable peptides to a larger extent than igmgo. in both cases the fragmentation resulted in immunoelectrophoretically pure fab mu and fc mu fragments. the fab mu fragments of human igm (yield: 20% of used igm material) had a molecular weight of 54,000, the fc mu f ...197995144
pesticide-induced alterations in the oxygen uptake rate of a freshwater major carp labeo rohita. 1979119624
trout mortalities as a result of streptococcus infection.excessive mortalities were experienced in the bigger rainbow trout (salmo gairdneri) at an efficiently managed trout farm. all tests for known toxins in the feed and water proved to be negative. a faecal streptococcus which belongs to the lancefield group d but which could not be identified as belonging to any of the recognized species within this group, was isolated from the spleens, livers and kidneys of affected fish. pathogenicity studies with this organism proved it to be highly fatal to tr ...1979121769
[regulations of igm immune response. iv. effect of the hapten: carrier ratio and the nature of the carrier on the intrinsic and functional affinity of carp dnp-antibodies].carp anti-dnp antibodies were raised by various dnp-carrier conjugates. their intrinsic affinity (k0) to monovalent e-dnp-l-lysine and functional affinity (df) for binding to the multivalent dnp-t4 bacteriophages were determined. the functional affinity of antibodies elicited by t-cell-dependent dmpn-hsa (n = 3, 15, 33) is relatively high (kf):1010-1012 m-1). these kf values increase more than k0 during the immune response. the functional affinity is dependent on the molar knp: hsa ratio. the me ...1979121787
peculiarity of temperature sensitivity of carp & rabbit myosins. 1979157328
nature of adenosine triphosphatase accelerating peptide from hydrolysate of fur seal muscle.ultrafiltered fur seal muscle hydrolysate was divided into eleven fractions by gel filtration on sephadex g-15. one of the fractions (fraction g9) accelerated the atpase activity of carp myosin b to a rate about two-fold faster than that of the control. fraction g9 showed a single ninhydrin spot in its silica gel thin layer chromatograph, and gave a positive test for tryptophan by the p-dimethylaminobenzaldehyde method, while tests for tyrosine, and for arginine were negative. the ion exchange a ...1979160912
resonance raman and epr of nitrosyl human hemoglobin and chains, carp hemoglobin, and model compounds. implications for the nitrosyl heme coordination state.we report the joint resonance raman (rr) and electron paramagnetic resonance (epr) study of five- and six-coordinate nitrosyl heme model compounds and of the titled nitrosyl hemoproteins. both epr and rr spectra fall into two types which, in the models, correspond to five- and six-coordinate nitrosyl hemes. however, neither rr nor epr spectroscopy is highly sensitive to the nature of the bond between a nitrosyl heme and a coordinated nitrogenous base, nor do the results of one technique uniforml ...1979220232
enzymes of steroid hormone metabolism in ovarian tissue of teleosts.1. substrate-velocity kinetics and some other characteristics of three enzymes involved in steroid hormone metabolism in the ovary of the common carp, cyprinus carpio and of the grey mullet, mugil cephalus were determined. 2. labeled steroid products of the enzyme reactions were identified by thin-layer and gas-liquid chromatographies, as well as by formation of derivatives. 3. 5 alpha-steroid reductase of carp ovary was found to have a michaelis-menten constant undistinguishable from that of ra ...1979318311
identification of spring viraemia in carp (cyprinus carpio l) in great britain.an investigation into a mortality of carp revealed a virus which was identified serologically and morphologically as being identical to svc virus. svc is a serious disease of european carp farms and this is the first positive identification of this disease in great britain.1979433114
[the sequence of beta a - and beta b-chains from carp hemoglobins (cyprinus carpio l.)].the primary structures of the beta a - and beta b-chains from the both main components of carp hemoglobins is given. the sequence was established by splitting with known methods and sequencing the products using the hydrophilic and hydrophobic programmes in the sequenator. the carp beta-chains differ from human beta-chains in the exchange of 66 and 67 amino acid residues, respectively; the beta a - beta b-chains differ by 4 amino acids (pos. no. 13, 41, 122 and 143). both chains have one inserti ...1979437706
ionic mechanisms of two types of on-center bipolar cells in the carp retina. i. the responses to central illumination.properties of the depolarizing response of on-center bipolar cells to a light spot stimulus were studied in the carp retina. on-center bipolar cells were classified into two types, cone-dominant and rod-dominant, according to their major input from cones and rods. cone-dominant bipolar cells responded to spectral light with the maximum amplitude near 625 nm, suggesting major input from red cones. the response was accompanied by a resistance increase and showed a reversal potential at -63 +/- 21 ...1979438766
[occurrence and ultrastructure of mast cells in the hypothalamus of teleost fishes].from 56 light-, fluorescence- and electronmicroscopically investigated brains of fishes mast cells could be found only in 4 cases. they were concentrated in the meninges of one pike (esox licius) and in the meninges and at larger blood-vessels of the hypothalamus of 2 trouts (salmo trutta fario). in the hypothalamus of a carp (cyprinus carpio) an infiltration of mast cells was detectable in the meninges, at blood-vessels and capillaries, between neuronal and glial fibres and sub- and intraependy ...1979473856
duplication 6q syndrome.duplication (partial trisomy) of the long arm of chromosome 6 has been described in 5 children [robertson et al, 1975, chen et al, 1976, clark, 1977]. we wish to report here an additional case due to a familial translocation in which the proband's karyotype is 46,xx,der(3),rcp(3;6)(p25;q21)mat. the phenotypes of the 6 children with duplication 6q are strikingly similar. each child has duplication involving approximately the distal 1/3 to 1/2 of the long arm of chromosome 6. distinctive features ...1979474632
anti-idiotypic antibodies of igm-type produced in carp (cyprinus carpio l.).carp synthesize highly specific anti-idiotypic antibodies of the igm class. ant-idiotypic antibodies could be elicited in these animals by a human igm myeloma protein and were detected in a passive hemagglutination assay. the agglutination was completely inhibited by approximately 0.15 microgram homologous antigen, whereas a 100 000-fold excess of a heterologous igm myeloma protein of the same l chain type did not produce any inhibition. a possible subgroup specificity of carp anti-idiotypic ant ...1979499328
non-repetitive dna sequence divergence in phylogenetically diploid and tetraploid teleostean species of the family cyprinidae and the order isospondyli.non-repetitive dna of anciently tetraploid teleostean species was analysed for the presence of duplicated sequences. closely related diploid species were investigated in comparison. from the reassociation kinetics of total nuclear dna, rate constants and fraction sizes of classes of repetitive and non-repetitive sequences were determined. dna fractions enriched in the slowest renaturing sequence class were determined. dna fractions enriched in the slowest renaturing sequence class were prepared ...1979533669
[carp-erythrodermatitis--isolation and classification of the agent (author's transl)]. 1979376269
laboratory assessment of the molluscicidal activity of b-2, a new chemical against oncomelania nosophora.laboratory trials of sodium 2.5 dichloro-4-bromophenol named b-2 for the molluscicidal effect against oncomelania nasophora were carried out by komiya's immersion technique. the values of lc50 of b-2 varied from 0.38 ppm to 0.54 ppm depending on the collection time of the snail. they were almost equal to those of napcp, but lower than those of niclosamide. in the tests of toxicity to carp, rainbow trout and killifish, b-2 showed lower toxicity than napcp. some aspects of mollusciciding applicati ...1979396400
5'-terminal sequences of spring viremia of carp virus rna synthesized in vitro.sequence analyses have been undertaken on the 5' termini of the rna species synthesized in vitro at 22 degrees c by spring viremia of carp virion (svcv)-associated transcriptase by using virus grown in mammalian bhk-21 cells. svcv product rna was synthesized in the absence or presence of low (0.56 mum) or high (0.8 mm) concentrations of added s-adenosyl-l-methionine (sam). two major sequences obtained in the absence (or in low concentrations) of sam have been shown to be gpppap and gpppampap(c). ...197916789178
[protein functional groups of the ova in the roach and carp-bream of varying degrees of maturity].the black sea roach and carp-bream eggs of different degree of maturity differ from each other by the content of sh-, s--s-groups of proteins and non-protein low molecular weight thiol compounds. definite differences were found in the optical density of soluble proteins and degree of ionization of their side groups.1979481851
dna reassociation kinetics in diploid and phylogenetically tetraploid cyprinidae.four diploid and three phylogenetically tetraploid cyprinidae (ostariophysi) have been characterized as for nuclear dna content, modal chromosome number and dna reassociation kinetics (hydroxyapatite chromatography). among the diploid species nuclear dna content (10(-12) g dna/2c) was 1.62 for tinca tinca, 1.87 for scardinius erythrophthalmus, 2.53 for leuciscus cephalus and 2.75 for alburnus alburnus, while the phylogenetically tetraploid species carassius auratus, barbus barbus and cyprinus ca ...1979490124
cytochemical differentiation of tonic (slow) and phasic (fast) muscle units in the ocular muscles of a fresh-water carp, catla catla (ham.).1. functional morphology of the ocular muscles with special reference to their topography, innervation and action have been studied in a fresh-water carp catla catla. an attempt has also been made to differentiate tonic (slow) and phasic (fast) muscle units in the various ocular muscles by their sdh activities and their lipid contents. 2. catla catla has large eyes (2 cm in diameter) in a fish measuring 30 cms. there are four recti (inferior, superior, anterior and posterior) and two obliquii (s ...1979516855
histological observation of some of the endocrine glands in the sterile carp-funa hybrid (f1), with special reference to the hypophysis.some endocrine glands of the carp-funa hybrids were studied with a light microscope to elucidate their detailed structure and the possible causal factor of sterility in the males. adult specimens of carp (cyprinus carpio), gengoroh-buna (carassius auratus cuvieri), and their hybrid (f1) were examined. the hybrid males are sterile as manifested by the failure of meiosis and seminomatous neoplasm in their testes. the hybrid females revealed well-developed ovaries, but their fertility was not teste ...1979539886
proprioceptive reflex connections of head musculature and the mesencephalic trigeminal nucleus in the carp.the location of the sensory cells concerned with the proprioception of respiratory and extraocular musculature in the carp was studied by retrograde transport of horseradish peroxidase. sensory cell labeling after intramuscular hrp injection was exclusively found in the trigeminal-facial-anterior lateral line ganglion complex. the muscles innervated by the trigeminal system are represented in the more rostral ganglion areas, the muscles innervated by the facial system in the more caudal ganglion ...1979762276
tactile input to the facial lobe of the carp, cyprinus carpio l. 1979227540
carp hemoglobin. v. effects of ph and p6-inositol on autooxidation.autooxidation of carp hemoglobin has been measured from 4--25 degrees c with and without p6-inositol. the rate was accelerated by the increase of proton concentration and/or the addition of p6-inositol. as the rate increases the kinetics become more complex. in acidic media with p6-inositol, the autooxidation is initially rapid which becomes slower subsequently but eventually proceeds very fast. the activation energy for autooxidation is about 19 kcal. mol-1. the complicated kinetics are partly ...197941592
[deriving requirements of major mineral elements in carp (caprinus carpio)]. 1979532382
mercapturic acid occurrence in fish bile. a terminal product of metabolism of the herbicide molinate.1. molinate sulphoxide, an oxidation metabolite of molinate, is cleaved in vitro by japanese carp liver cytosol fraction, indicating the presence of gsh-s-transferase activity, since cleavage of the sulphoxide is dependent on the amount of supernatant protein and gsh in the assay medium. 2. molinate mercapturate was identified as a major metabolite recovered from bile of carp treated with molinate. 3. the structure of the mercapturate was established by co-chromatography with an authentic standa ...1979532216
[change in ribose levels in the meat of stored carp].to study the changes in ribose levels in carp meat during storage, total ribose soluble in acids was determined, ribose precipitating by barium hydroxide (free ribose), the ratio of these two values, and determination from the alkaline extract of zinc sulphate was also used. already in freshly suffocated carp relatively high levels of acid-soluble ribose were found as well as free ribose. the even increase of pentose in the alkaline extract of zinc sulphate allows to use the procedure for the ev ...1979117605
[the suitability of some chemical methods for evaluation of freshness of stored suffocated carp].for the assessment of the degree of meat freshness of stored unbleeded carp some simple chemical methods were tested to be used as auxiliary criteria for veterinary decisions regarding the edibility. under the experimental conditions where the suffocated carp were stored at cooling and room temperatures in water or in the air, differences were determined in the followed criteria between fresh and deteriorating fish which were statistically insignificant for most indicators. in view of requiremen ...1979117604
suppression of retinal spike discharge by dipropylacetate (depakene): a possible involvement of gaba.effects of dipropylacetate (dpa) were studied on spike discharges in the isolated carp retina. (1) dpa, applied electrophoretically or by pressure-microinjection in the vicinity of the recording electrode at the inner plexiform layer, consistently activated spike discharges. (2) dpa introduced into the perfusate produced complex actions; some units responded to dpa with an initial activation followed by suppression of spike discharges. (3) when gamma-aminobutyric acid (gaba) was applied electrop ...1979226226
the ulcus-syndrome in cod (gadus morhua). iii. a preliminary virological report.a bullet shaped virus presumably belonging to the rhabdoviridae was isolated from a pool of 7 cods (gadus morhua) with the ulcus-syndrome. this virus measured 55 x 175 nm. the virus caused cpe in ps (pike sarcoma) cells, but not in a number of other cell lines. from the same pool another virus was isolated in epc (epithelia carp) cells. this virus was an icosahedron of 145 nm and most likely to be placed in the family iridoviridae. electronmicroscopic examination of papules from another 13 cods ...1979392451
a histochemical study on the innervation of the cerebral blood vessels in the carp.adrenergic and cholinergic innervation of the cerebral blood vessels were studied in the carp using fluorescence and cholinesterase histochemistry. most of the major cerebral and pial arteries were densely innervated with both nerves. moreover, both nerves, being probably of central origin, were observed frequently on the walls of parenchymal arterioles and capillaries in the sections of the inferior lobe but were only rarely found in the other brain sections.1979488291
topographical relation between olfactory bulb and olfactory tracts in the carp. 1979487076
[gill disease (candidamycosis) in carp]. 1979483550
histochemical studies on catecholaminergic cells in the carp retina.histochemical studies on catecholaminergic cells were conducted with the carp (cyprinus carpio) retina. catecholamine (ca)-containing cell bodies appear sparsely distributed among amacrine cells in the innermost cellular row of the inner nuclear layer (inl) and occasionally in the outer half part of the inner plexiform layer (ipl); only exceptionally are they found among ganglion cells. the fluorescent cells interspersed with the amacrine cells and in the ipl send their fiber processes toward bo ...1979481681
studies on two types of gonadotropins from both salmon and carp pituitaries. 1979478277
thermoacclimatory changes in the ionic microenvironment of haemoglobin in the stenothermal rainbow trout (salmo gairdneri) and eurythermal carp (cyprinus carpio).1. haematological characteristics (erythrocyte number, haematocrit, haemoglobin, mean erythrocyte volume and haemoglobin content) and plasma and packed red blood cell water and electrolyte (na+, k+, ca2+, mg2+, cl-) levels were determined in summer and winter populations of rainbow trout acclimated to 2, 10 and 18 degrees c, and for carp held at 2, 16 and 30 degrees c. erythrocyte electrolyte concentrations and ion:haemoglobin ratios were calculated from these data. 2. modest increases in red ce ...1979501276
offset rate of action of muscarinic antagonists depends on their structural flexibility.time course measurements of the action of muscarinic antagonists were performed in the spontaneously beating carp atrium. several high affinity drugs, which embody the quinuclidine structure were examined. the structural flexibility of these molecules was reflected in the dissociation of the drugs from the muscarinic receptor. the dissociation of rigid drugs was very much prolonged as compared to flexible drugs of the same affinity.1979446670
electromechanical noise in atrial muscle cells of the carp: a possible ionic feed-back mechanism.1. spontaneous electrical fluctuations (+/- 0.2-0.4 mv) were recorded in 'quiescent' atrial fibres of the carp. 2. the noise decreased in acetylcholine, small hyperpolarization and egta. 3. the noise increased (to +/- 1-2 mv) and became more synchronous in k+-free ringer and in ouabain. 4. large voltage fluctuations (+/- 1-2 mv) were accompanied by a fine mechanical tremor, indicating intracellular [ca2+] fluctuations. 5. spectral analysis showed a clear resonant frequency at about 1 hz, indicat ...1979469777
a dynamic system for long-term toxicity studies in fish under laboratory conditions.for the evaluation of possible adverse effects of compounds in fish at sublethal doses a dynamic system is required. the design of such a system is presented including the methodology of performing long-term toxicity studies in fish using selected environmental chemicals (plant protection products). chronic studies with diazinon, terburyn, phosphamidon on carp and trout comprised 28 day treatment periods followed by 28-day recovery periods. in addition to the toxicity, the residues of the applie ...1979288356
[studies of the antibody heterogeneity in carp (cyprinus carpio l.). i. electrophoretic and isoelectric spectra of anti-dnp-antibodies].carp igm as well as carp anti-dnp-antibodies migrate electrophoretically very well as a diffuse band into polyacrylamide gel of large pore size. the isoelectric spectra of the carp anti-dnp-antibodies are heterogeneous and show bands in the pi-range of 4.0 to 6.4. the activity of focused anti-dnp-antibodies could be demonstrated in the pi range between 5.4 to 6.4 even in high antibody dilutions. the investigated structural heterogeneity of the anti-dnp-antibodies of carp is a further proof for t ...197945163
a rhabdovirus isolated from carps in hungary. experimental infection of carps and resistance of the virus.from carps showing the symptoms of acute infectious dropsy, a virus was isolated for the first time in hungary. on the basis of physicochemical and serological tests and electron microscopy, the virus was identified as spring viraemia carp virus and assumed to have a primary role in the acute form of infectious dropsy known so far as a bacterial disease.197944429
[localization of immunoreactive sites with anti met-enkephalin and anti alpha-endorphin sera in carp brain].in the brain of the carp an anti met-enkephalin serum reveals some telencephalic fibres, about half of the n.p.o. cells and furthermore a subependymal zone of nervous tissue close to the third ventricle of the superior hypothalamus and thalamus. these structures do not react with an anti alpha-endorphin serum, which however reveals cells of the lateral n.l.t. and the corresponding fibres.1979111832
a comparison of the toxicity of nickel to the developing eggs and larvae of carp (cyprinus carpio). 1979444757
[carp valiporosis]. 1979442512
distribution of superoxide dismutase and glutathione peroxidase in the carp: erythrocyte manganese sod. 1979435318
[immunocytologic differentiation of hypophyseal innervation in carp using anti met-enkephalin and anti-alpha-endorphin serums].the immunocytological reactions with anti-met-enkephalin and anti-alpha-endorphin reveal two types of fibres in the intermediate lobe of the carp. their pathways through the adenohypophysis and the localization of their terminals are different. the anti-met-enkephalin positive fibres terminate mainly in a zone close to the proximal pars distalis; however some fibres terminate deeper in the intermediate lobe. the anti-alpha-endorphin positive fibres penetrate mainly in the central and ventral par ...1979111863
rod- and cone-bipolar cell responses in the carp retina. 1979446559
the effect of gonadotrophins on the steroidogenesis, in the ovary and testis of gonadotrophin-deprived fresh water teleost, cyprinus carpio.studies have been made on the effect of lh, fsh, and lh+fsh on the gonadal steroidogenesis in gonadotrophin-deprived common carp. lh alone and in combination with fsh was more effective than fsh in stimulation of steroidogenesis.1979421781
effects of exogenous neuraminidase on unit activity in frog spinal cord and fish optic tectum.the effect of exogenous neuraminidase on spontaneous and evoked synaptic activity was investigated following injection of the enzyme into frog spinal cord and into cord and into the carp optic tectum. 1. injection of enzyme into spinal cord segments caused a significant increase of motoneuron activity in the corresponding spinal nerve, which lasted for 7--31 min. 2. the amplitude of postsynaptic evoked potentials in the optic tectum was increased after neuraminidase injection up to 200% of contr ...1979215438
hydrolysis and synthesis of wax esters by different systems of carp hepatopancreas preparation.it was found that the supernatant of hepatopancreas homogenate of the carp, cyprinus carpio, contained enzymes involved in the hydrolysis and esterification of wax esters. the enzymes were salted out by 30% saturation with ammonium sulfate. this fraction also contained the highest activities of lipase and esterase. the enzyme for the hydrolysis of wax esters, together with lipase and esterase, was eluted in tube numbers 20 to 22 (void volume) from a sephadex g-200 column. on the other hand, the ...1979762038
the hematology of mrigal, cirrhina mrigala (ham.) (teleostei: cyprinidae).hematological parameters of a carp, cirrhina mrigala, are reported here. haematocrit ranged from 29.0 to 44.0%. the range of hemoglobin was 7.06 to 11.86 g/100 ml of blood, and the total number of erythrocytes varied from 1.7 to 3.0 million/mm3 of the blood, both hemoglobin and erythrocyte concentrations being higher in males than in the females. mean esr was 1.88 mm/h, with higher esr in females (2.05 mm/h) than in the males (1.67 mm/h). the clotting time in males (41.0 seconds) was lower than ...1979543563
the surface connected canalicular system of carp (cyprinus carpio) thrombocytes: its fine structure and three-dimensional architecture.the presence and the three dimensional distribution of the surface connected canalicular system (sccs) in thrombocytes of a teleost, cyprinus carpio, were studied using a transmission electron microscope, a high voltage electron microscope and a scanning electron microscope. when the specimens were fixed routinely in glutaraldehyde followed by osmium tetroxide, numerous electron lucent vesicles and canaliculi were distributed throughout the cytoplasm. as ruthenium red-positive reaction product w ...1979519726
calcium and magnesium binding by parvalbumin. a proton magnetic resonance spectral study.proton magnetic resonance spectroscopy has been used to monitor the kinetics and nature of the conformational transitions induced by binding of calcium and magnesium to carp parvalbumin. rate constants have been determined for the exchange between the cation dependent conformational states of the protein in solution. these data enable a description of the kinetics and mechanism controlling the calcium flux in vivo during contraction.1979518923
myxobolus pavlovskii (achmerov, 1954) (myxosporidia) infection in the silver carp and bighead. 1979517353
horizontal cells of the carp retina: golgi impregnation and procion-yellow injection.application of two methods for the selective staining of neurons, golgi impregnation and intracellular marking with procion yellow, has revealed the anatomical arrangements of the horizontal cells in the carp retina. there are two basic horizontal-cell types, those with axons and these without. the former can be subdivided into three groups on the basis of the pattern of branching of the dendrites. these three structural groups are also functionally distinct, as shown by the electrical recording ...197883194
synchronized rhythmic discharges of the secondary olfactory neurons in carp.(1) rhythmic and synchronized multiunit bursts of spike discharges, which were superimposed on the peaks of the oscillatory potentials of 4--10 hz (olfactory tract waves), were induced in the olfactory tract fibers of the carp in response to olfactory stimulation. suppression of the spike discharges was observed during the interburst periods of discharges. (2) single-unit analysis revealed three types of discharge patterns in the olfactory tract fibers in response to olfactory stimulation: rhyth ...1978709369
convergence of rod and cone signals to single bipolar cells in the carp retina. 1978755293
receptive field arrangement of color-opponent bipolar and amacrine cells in the carp retina.seven color-opponent bipolar and 50 color-opponent amacrine cells were identified in the carp retina and their spectral responses examined by using procion-dye-filled micropipets for staining and recording. four of the bipolar cells had a +r-g-and three a +y-b-type chromatic center. six of the bipolar cells showed hyperpolarizing non-color-opponent surrounds, while one +r-g-type cell had a +y-rb-type surround. this cell showed the greatest complexity of all bipolar cells identified so far, and i ...1978755292
ionic mechanisms underlying the center and surround responses of on-center bipolar cells in the carp retina.reversal potentials were measured for the center and surround responses of on-center bipolar cells in the carp retina. in the scotopic condition, the center response showed a reversal at 29 +/- 13mv, the surround response at 38 +/- 13 mv. the result suggests that both the center and surround responses were -53 +/- 11 mv and -59 +/- 11 mv, respectively. these responses were sensitive to the red light. it is therefore suggested that both the center and surround responses contributed by rods are me ...1978755291
effects of gaba on neuronal activities in the distal retina of the carp.when a transient depolarization was evoked in horizontal cells by a transretinal current pulse, a small hyperpolarizing deflection (probably ipsp) was detected in cones. the ipsp was abolished by superfusion of the retina with gaba-containing ringer solution, which also hyperpolarized the cones. these results suggest that, in the dark, a gaba-mediated negative feedback was operating from horizontal cells to cones, and terminated in the light. application of gaba also caused remarkable changes in ...1978755289
electrically evoked responses (e-responses) of l-and c-type horizontal cells in the carp retina.electrically evoked responses (e-responses) of l-and c-type horizontal cells produced by transretinal stimulation were studied under chromatic illumination in the carp retina. from the examination of e-s relations (e-response verus s-potential) and of the shape of both responses the following results were obtained: (1) equilibrium potential of subsynaptic membrane is estimated as 0 to 10 mv above zero membrane potential. (2) l-cells receive signals from red-and probably from green-sensitive cone ...1978755288
[on--off impulse activity of carp retina amacrine cells].properties of spontaneous and light-evoked spikes of the "on--off" amacrine cells of the carp retina were studied. it was assumed that the dendrites of amacrine cells generated spike potentials and conducted them to the cell body. the data obtained suggest that the dendrites of amacrine cells have many spike-generation zones and that the transient depolarization component of the amacrine response to light is the compound spike consisting of many separate spikes generated in different parts of th ...1978744273
gas exchange in the carp gills in normoxic and hypoxic conditions.respiratory exchange measurements in a teleost fish, cyprinus carpio, in normoxic and hypoxic conditions are presented. oxygen pressure in the dorsal aortic blood in normoxic carp was much lower than that in most of teleosts and elasmobranchs in similar conditions. the most evident physiological adjustment to the hypoxic condition was the increased ventilation due to an increased frequency of operation of the branchial pump. increased perfusion at the gills played a small role in the physiologic ...1978741107
studies on the mechanisms underlying horizontal-bipolar interaction in the carp retina.responses of on-center bipolar cells and horizontal cells were recorded simultaneously in the carp retina, and the effect of polarization of horizontal cells on the bipolar cells was studied. hyperpolarization by extrinsic current of horizontal cells elicited in the bipolar cells a hyperpolarizing response which, unlike the electrical coupling betweeen adjacent horizontal cells, was accompanied by a change in membrane conductance. the bipolar cell responses elicited by polarization of external h ...1978225825
abnormality in body curvature of cyprinus carpio after injection of anaesthetics.abnormalities in the body curvatures were noticed in cyprinus carpio after the injection of anaesthetics, viz. paraldehyde, tertiary butyl alcohol and butanol. these abnormalities occurred behind the dorsal fin and progressively developed over a period of four months. the abnormalities affect the locomotion and speed of swimming of the fish and appear to be due to toxic effects of the anaesthetics.1978742410
[interrelationships of the neurosecretory cells and capillaries in the preoptic nucleus of the carp].three layers are tentatively recognized in the wall of a blood capillary of nucleus praeopticus (np) of the carp, cyprinus carpio l: the inner layer--endothelium, the middle layer--basement membrane with pericytes, and the outer broken layer containing mostly leucocytes and fibroblast-like cells. 1). the plasmalemma of the neurosecretory cells (nsc) makes a direct contact with a capillary: (a) with the plasmalemma of a sole-like process of the endothelial cell (0.1%), (b) with the basement membr ...1978734769
occurrence of catecholamine-containing specific granules in the venous endothelia of carp.the formaldehyde-induced fluorescence technique and the argentaffin reaction with ultra-thin sections were applied to the vessels of carp to demonstrate the presence of a catecholamine. after formaldehyde gas treatment for 30 minutes at 50 degrees c, the venous endothelia emitted a greenish-yellow fluorescence suggesting the presence of a primary catecholamine. in the endothelial cells of veins, the specific granules, consisting predominantly of spherical granules with a diameter of about 0.3 mi ...1978707309
[cytochemical studies on blood cells of 3 teleost species (carp--cyprinus carpio l., rainbow trout--salmo gairdneri richardson and pike--esox lucius l.)]. 1978697717
olfactory neuroepithelioma in a domestic carp (cyprinus carpio).a well-differentiated olfactory neuroepithelioma was found protruding from the left olfactory fossa of a 6-year-old domestic multicolored carp, cyprinus carpio. following diagnostic biopsy the neoplasm continued to grow. at necropsy, the tumor was found to extend beyond the olfactory chamber into the intracranial portion of the olfactory bulb, but no distant metastases could be detected. histologically, the neoplasm had a remarkably organoid appearance, being composed of imperfect reproductions ...1978698944
the effect of fenitrothion on reproduction of a teleost fish, cyprinus carpio communis linn: a biochemical study. 1978719191
afferent and efferent fiber connections of the carp torus longitudinalis.the efferent and afferent pathways of the carp torus longitudinalis were studied by means of degeneration and retrograde hrp methods. efferent projections were only seen in the most superficial layer of the ipsilateral optic tectum (stratum fibrosum marginale). afferent pathways to the torus longitudinalis were found to originate mainly in the valvula cerebelli. degenerating fibers course in the tractus mesencephalocerebellaris posterior within the valvula, and join the tractus mesencephalocereb ...1978690274
genetic improvement of wild fish populations.a plan for the genetic improvement of commercially exploited wild animals is presented. it consists of crossing wild with domesticated breeds to produce heterotic hybrids and to upgrade the wild stocks. empirical evidence is presented from experiments with the carp. procedures for monitoring the manipulated populations are outlined. the suggested plan is ecologically reasonable and would counteract the negative genetic changes caused by excessive commercial exploitation of many species.197817830305
further evidence suggesting that the slow phase in protein unfolding and refolding is due to proline isomerization: a kinetic study of carp parvalbumins. 197830472
autonomic response of the fish to pyrogen.lipopolysaccharide (lps) applied to the anterior brainstem of the carp caused lightening of body colour. this indicates that an increase in set point temperature is responsible for increased cutaneous autonomic activity following lps-administration.1978720514
[speed of in vitro reassociation of gonadotropin subunits in a teleost fish, the carp (cyprinus carpio l.)].purified gonadotropin subunits of a teleost fish, the carp, reassociated in vitro, according to a second order rate reaction. the rate constant was temperature dependent. compared to ovine lh, the q10 was about three times less and the rate constant much greater (160 times for instance at 20 degrees c). the possible physiological significance of these differences is underlined.1978102442
effects of vitamin a administration upon ito's fat-storing cells of the liver in the carp.in the normal carp livers, ito cells (fat-storing cells) could be identified with light and electron microscope within the hepatic sinusoidal wall and actually in the disse's space, but they were almost lacking lipid droplets in the cytoplasm (empty ito cells) in contrast to the majority of vertebrate species. after administration of large doses of vitamin a, fat droplets appeared in hypertrophic cytoplasm of enlarged ito cells, and their size and number increased roughly in proportion to the am ...1978718387
[responses of carp olfactory receptors to different classes of chemical substances]. 1978680284
telencephalic afferent neurons identified by the retrograde hrp method in the carp diencephalon. 1978306862
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