Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
studies on the disaggregation of ef-1 with carboxypeptidase a. 1977406850
isolation and characterization of structural components of bacillus cereus ahu 1356 cell walls. 1977407078
the action of lysozyme on peptidoglycan with n-unsubstituted glucosamine residues. isolation of glycan fragments and their susceptibility to lysozyme.1. a peptidoglycan preparation n-acetylated at about 30% of glucosamine residues was obtained by the treatment of the lysozyme-resistant cell wall paptidoglycan of bacillus cereus with acetic anhydride at ph 7. fractionation of dialyzable material resulting from lysozyme digestion of the glycan component of this peptidoglycan preparation yielded five oligosaccharides designated as s1 to s5 besides the disaccharide glcnac-murac. 2. oligosaccharide s3, which accounted for about 30% of the disaccha ...1977407083
spores of microorganisms. xxvii. some physiological and morphological characteristics of thick-walled cellular forms of bacillus cereus. 1977407136
microbiol n-acetylation of daunorubicin and daunorubicinol. 1977407204
production of copper coproporphyrin iii by bacillus cereus. i. purification and identification of copper coproporphyrin iii.bacillus cereus strains 2 and t did not form spores and accumulated a large amount of purple pigment inside the cells, when cultured in a yeast extract-ammonium salt medium with excess glucose. the pigment was extracted and crystallized as the ethyl ester. it was identified as copper coproporphyrin iii.1977407221
production of copper coproporphyrin iii by bacillus cereus. ii. regulation of the biosynthesis of coproporphyrin iii and its copper complex by oxygen and heavy metal ions.the effects of oxygen and heavy metal ions on the production of copper coproporphyrin iii were studied in bacillus cereus strain 2. the formation of copper coproporphyrin iii was found to be maximum when the cells were cultivated in g-medium at a low level of oxygen supply, but it was suppressed at extremely low oxygen supply levels. when the cells were cultured in metal-free g-medium, neither metal-coproporphyrin iii nor coproporphyrin iii was formed. in the presence of copper in the medium (40 ...1977407222
a case of bacillus cereus bacteraemia.a case is presented of bacillus cereus bacteraemia in a patient receiving hepatic perfusion with 5-fluorouracil (5fu) for metastasis from a carcinoma of the breast. the literature concerning systemic b. cereus infections is briefly reviewed.1977407561
[detection of bacillus cereus-toxins (author's transl)].during the logarithmic state of growth, b. cereus produced extracellular toxins, which could be precipitated by ammoniumsulphate. the toxins were not dialysable. 9 strains of b. cereus were tested and showed lethal, hemolytic, and phospholipase-c-reactions. the lethal toxin injected i.v., killed mice within 20 minutes. an intradermal injection of the 9 toxin-precipitates in rabbits produced skin reactions, which could be neutralized by a specific antiserum against the b. cereus strain b-4ac. rel ...1977407743
inhibition of the synthesis of phospholipase c in bacillus cereus by a component of the growth medium.a low molecular weight compound, probably of peptide nature, present in the beef heart extract component of the growth medium, inhibits post-transcriptionally the biosynthesis of phospholipase c by a strain of bacillus cereus. the compound also prevents the increase of proteolytic activity in the growth medium, thus suggesting that the synthesis of another enzyme, an extracellular protease, is also inhibited, and that the inhibitory compound may therefore have a more general effect on exoenzyme ...1977407774
epoxidation of aldrin to exo-dieldrin by soil bacteria.twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively.1977407844
studies on bacterial cell wall inhibitors. ii. inhibition of peptidoglycan synthesis in vivo and in vitro by amphomycin. 1977407940
[regulation of isoleucine and valine biosynthesis in bacillus cereus t: possible role of threonine deaminase].threonine desaminase: a possible regulatory element suggested by the analysis of isoleucine, valine pathway regulation in bacillus cereus t.1977408042
re-activation of guinea-pig liver uridine diphosphate glucuronosyltransferase with 1-monoacyl phosphatidylcholine after extensive phospholipid deletion at 5 degrees c with pure bacillus cereus phospholipase c. 1977408208
[heterogeneity of the microcyclic development of bacillus cereus 504]. 1977408588
studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in galleria mellonella.cellular aggregates or nodules which formed in galleria mellonella larvae in response to injections of killed bacillus cereus were examined during their first 24 hr of development. the initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. this material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ...1977408940
antimicrobial activity of amine oxides: mode of action and structure-activity correlation.the effect of n-alkyl derivatives of saturated heterocyclic amine oxides on the growth and metabolism of microorganisms has been studied. 4-dodecylmorpholine-n-oxide inhibited the differentiation and growth of bacillus cereus, of different species of filamentous fungi, and of the yeast saccharomyces cerevisiae. for vegetative cells, the effect of 4-dodecylmorpholine-n-oxide was lethal. cells of s. cerevisiae, after interaction with 4-dodecylmorpholine-n-oxide, released intracellular k(+) and wer ...1977409340
extent of cross-linking of germ cell wall of a variant of bacillus cereus. 1977409409
immunocytochemical localization of spore specific antigens in ultrathin sections. 1977409707
[a fatal food poisoning caused by bacillus cereus (author's transl)].the autopsy of a 37 year old man who had died under extraordinary circumstances showed a bromatoxism by bacillus cereus. the verification of the bacillus was possible although the man had already died 2-3 days before; and that in spite of the existence of early beginning rottenness. with the help of morphological findings the pathogenetic effect of the toxins (exotoxin and enterotoxin) of bacillus cereus is discussed. blood cultures of the heart alone are not sufficient to prove a causal connexi ...1977410193
comparative studies of bacillus cereus strains isolated from various foods and food poisoning outbreaks. 1977410239
effect of some bacterial products on platelet electrophoretic mobility (pem).bacterial lps, used in our experiments, are able to decrease pem at concentration of 100 microgram/ml after incubation with 1 x 10(7) platelets/ml for 30 min at 37 degrees c. such effect is statistically significant for bacteroides fragilis lps, s. enteritidis and s. typhi. e. coli lps 026:b6 w and e. coli 055:b5 w are not effective at all, s. minnesota (incomplete) lps 595 and 345 free of 0-polysaccharide and partially lacking core in their molecule did not influence pem, while s. minnesota lps ...1977410424
characterization of the "microprotease" from bacillus cereus. a zinc neutral endoprotease.the neutral protease isolated from bacillus cereus (brl-70) has been purified by affinity chromatography and characterized. the enzyme exhibits a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has a molecular weight of 34 000 by ultracentrifugation, and contains one enzymatically essential zinc atom per 34 000 g. these data together with the amino acid composition, response to metal substitution, chemical modification, and substrate specificity all indicate that this p ...1977410441
levels of acetyl coenzyme a, reduced and oxidized coenzyme a, and coenzyme a in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of bacillus megaterium.dormant spores of bacillus megaterium were found to contain approximately 850 pmol of coenzyme a (coa) per milligram of dry weight. of this total, less than 1.5% was acetyl-coa, 25% was coa-disulfide, 43% was in disulfide linkage to protein, and the remainder was the free thiol. dormand spores of bacillus cereus and clostridium bifermentans contained 700 and 600 pmol of coa per milligram of dry weight, respectively; in both species approximately 45% of the coa 45% of the coa was in disulfide lin ...1977410791
[the occurrence of bacillus cereus in dairy products (authors transl)].during one year 1390 samples of pasteurized milk products from five different dairies were examined for content of b. cereus. the examination was done on the day of packaging and after incubation at 17 degrees c for 24 hrs. as well. on examination on the day of packaging 8.1 % were positive. no difference could be demonstrated between low-temperature pasteurized and high-temperature pasteurized products. however, differences were found from one plant to another (2.1 %--14.5 %). after incubation ...1977411110
the fate of enterotoxigenic bacteria inoculated onto chinese barbecued foods. 1977411566
[detection of cephalosporins in raw milk with the aid of b. cereus beta lactamase]. 1977411648
relationship between the structure of carbonylcyanide phenylhydrazones and inhibition of growth of microorganisms, stimulation of respiration of yeast cells and rat liver mitochondria.the effect of ten derivatives of carbonylcyanide phenylhydrazone on growth of bacteria, yeast and different species of filamentous fungi was investigated. in yeast and mitochondria isolated from rat liver the effect of these derivatives on the respiratory activity was also followed. the relative efficiency of the individual derivatives of carbonylcyanide phenylhydrazone was determined on the basis of the results obtained. it was shown that derivatives, in which the substituent on the benzene rin ...1977411721
a survey for the presence of teichuronic acid in walls of bacillus megaterium and bacillus cereus. 1977411891
[phage diagnosis of strains of bacillus cereus isolated from food products].a total of 80 cultures of bacillus cereus, isolated from food products, were studies to establish the behaviour to specific phages or the cam set and the presence of lysogeny. a modern bacteriophage method was tested for the first time in the differentiatons of bacillus cereus, which was so far studied mainly in the differentiation of bacillus anthracis. results showed that bacteriophagy with specific type phages as a method, though not absolutely precise, is dependable for the differentiation o ...1977412296
characterization and function of intracellular proteases in sporulating bacillus cereus.intracellular proteases from sporulating bacillus cereus have been purified by ammonium sulfate fractionation, heat treatment and deae cellulose column chromatography. after the last purification step, two protease activities, with an activity ratio of about thirty to one are resolved. both proteases are resistant to o-phenanthroline but sensitive to phenyl methyl sulfonyl fluoride. their separation by polyacrylamide gel electrophoresis and deae cellulose column chromatography, their difference ...1977412478
[the effect of sodium fluoride on the antimicrobial action of tetracycline]. 1977412595
[hygienic evaluation of insecticide preparations]. 1977412732
the effect of water activity and aw-controlling solute on sporulation of bacillus cereus t. 1977412827
membrane properties of aplysia neurones intracellularly injected with phospholipases a and c.1. the effects of phospholipases a from bee venom and from porcine pancreas and of phospholipases c from clostridium welchii and bacillus cereus on active and passive membrane properties of aplysia neurones have been studied. consistent alterations in electrical membrane properties were found following intracellular application of three of these enzymes.2. bee venom phospholipase a produced a rapid decrease of membrane potential and resistance. voltage clamping revealed a marked depression of pe ...1977874892
substrate-induced deactivation of penicillinases. studies of beta-lactamase i by hydrogen exchange.the conformational motility of beta-lactamase i from bacillus cereus was studied by hydrogen exhange. the time course of the isotopic replacement of peptide hydrogen atoms was followed by 'exchange-in' or 'exchange-out' experiments. many of the substrates for this enzyme that have o-substituted aromatic or heterocyclic side chains (e.g. methicillin or cloxacillin) are known to effect a decrease in enzymic activity ('substrate-induced deactivation'). there was a marked discontinuity in the exchan ...1977921750
asymmetry of the phospholipid bilayer of rat liver endoplasmic reticulum.the phospholipids of intact microsomal membranes were hydrolysed 50% by phospholipase c of clostridium welchii, without loss of the secretory protein contents of the vesicle, which are therefore not permeable to the phospholipase. phospholipids extracted from microsomes and dispersed by sonication were hydrolysed rapidly by phospholipase c-cl. welchii with the exception of phosphatidylinositol. assuming that only the phospholipids of the outside of the bilayer of the microsomal membrane are hydr ...1977921959
evidence for two immune inhibitors from bacillus thuringiensis interfering with the humoral defense system of saturniid pupae.mutants of bacillus thuringiensis lacking either beta-exotoxin or gamma-endotoxin were compared for their virulence using pupae of a giant silk moth. known doses of viable log-phase bacteria were injected, and the response was followed as the number of viable bacteria in the hemolymph. the results obtained imply that, in the system used, neither the beta-exotoxin nor the gamma-endotoxin and the sporeforming ability are of importance for virulence. results with sterile culture filtrate from b. th ...1976992874
parenteral administration of phospholipase c in the rat. distribution, elimination, and lethal doses.infusions of tissue thromboplastin induce intravascular coagulation in animals. phospholipase c (plc) (ec 3.1.4.3) from bacillus cereus has a marked protective effect against such infusions and might be of value in the therapy of certain types of intravascular coagulation. we have therefore studied the toxicity, half-life, and effect on lipolysis of purified plc in rats, using parenteral administration of 14c-labelled enzyme. following intravenous injection, the plasma half-life was 5.2-5.4 min, ...19761006144
modified assay medium for the turbidimetric assay of chlortetracycline in feeds.in previous experiments, the turbidimetric method for determining chlortetracycline-hci (ctc-hci) in feeds showed lower recovery than the aoac plate method. although the addition of vitamins to the turbidimetric medium improved results, values by the turbidimetric method remained about 10% lower than by the plate method. a modified (1.7x the weight recommended by the manufacturer) turbidimetric assay medium decreased assay sensitivity but did not significantly change the slope of s. aureus respo ...1976818071
the surface morphology of the phagocytosis of micro-organisms by peritoneal macrophages.scanning electron-microscopic studies of phagocytosis of various micro organisms by murine peritoneal macrophages has been made. bacillus cereus, staphylococcus aureus, and brewer's yeast were easily ingested, whereas cryptococcus neoformans was not. the development of intricate and tenuous filopodia preceded close-surface attachment, after which both thin and thicker lamellipodia invested the micro-organisms. loss of the normal surface ridges characterised the replete cell. the technique of sca ...1976818354
quantitative structure-activity relationships. 2. a mixed approach, based on hansch and free-wilson analysis.based on the theoretical and numerical equivalence of hansch's linear multiple regression model and the modified free-wilson model a mixed approach is developed. the mixed approach is a combination of both models which makes use of the advantages of each model and widens the applicability of hansch and free-wilson analysis. the free-wilson approach now is applicable also in the case of parabolic dependence of biological activity on a particular physical property, e.g., log p or pi. a rational ex ...1976818381
isolation of three new antibiotics, thiocillins i, ii and iii, related to micrococcin p. studies on antibiotics from the genus bacillus. viii.thiocillins i and ii were isolated from the culture broth of bacillus cereus g-15, and thiocillins ii and iii from that of bacillus badius ar-91. also, the former two were probably produced by bacillus megatherium i-13. these antibiotics active against gram-positive bacteria are soluble in a mixture of chloroform and methanol, show characteristic ultraviolet absorptions (maxima at ca. 275 nm and ca. 348 nm), and contain a high content of sulfur, as much as approximately 15%. they are related to ...1976819410
the effect of phospholipase c on human blood platelets.the effect of phospholipase c (ec 3.1.4.3) on human blood platelets has been studied. phospholipase c from bacillus cereus was purified to homogeneity as judged by analytical and sodium dodecyl sulphate disc gel electrophoresis and by immunoelectrophoresis. human platelets isolated from platelet-rich plasma by gel filtration or by centrifugation and washing were incubated with phospholipase c. a loss of 20-45% of the total platelet phospholipid was observed, whereas 88% was hydrolyzed when plate ...1976819457
an outbreak of bacillus cereus food-poisoning in finland associated with boiled rice.a food-poisoning outbreak caused by bacillus cereus occurred in a finnish industrial plant in january 1975. eighteen of the 36 persons who ate a lunch including boiled rice, meat and vegetables became ill. the disease pattern was similar to previously reported short incubation time b. cereus food-poisonings associated with cooked rice. the median incubation time was two hours, the main symptoms being nausea, abdominal pain and vomiting. rice and certain seasonings were the contaminated raw mater ...1976819571
an outbreak of bacillus cereus food poisoning resulting from contaminated vegetable sprouts.in an outbreak of gastrointestinal illness caused by consumption of home-grown raw vegetable sprouts contaminated by bacillus cereus, victims developed symptoms after an incubation period of 6-15 hours. four persons initially experienced nausea and vomiting, and this was followed in 3 cases by abdominal cramps and diarrhea. bacteriologic investigation indicated that b. cereus on unsprouted seeds proliferated during germination in a commercially sold seed sprouting kit and reached levels in exces ...1976820192
[an episode of alimentary toxinfection caused by bacillus cereus]. 1976823623
[antibiotic residues in organs and muscle tissues of broilers. i. bacitracin, flavomycin, spiramycin and viriniamycin residues following administration of diets containing low levels of these antibiotics (author's transl)].two groups of broilers were fed two different feed mixtures. a feed containing a mixture of bacitracin, flavomycin, spiramycin and virginiamycin (20 ppm each) was administered to sixity broilers. sixty other broilers were given a similar feed not containing any antibiotics. after slaughter, samples of kidney, liver and breast were examined for the presence of antibiotic residues. all samples were found to be negative for antibiotic residues. four micro-organisms were used in performing the tests ...1976823664
effect of ultrasonic waves on the heat resistance of bacillus spores.the effect of ultrasonic treatment on the heat resistance of bacillus spores differs widely both with the species and the strain tested. ultrasonic waves do not affect the heat resistance of some strains, whereas they greatly reduce that of other strains of the same species. the heat-sensitizing effect of ultrasonic waves is more pronounced when short heating periods are used. ultrasonic treatment does not seem to affect the "z value".1976823864
heat-induced requirements for sucrose or magnesium for expression of heat resistance in bacillus cereus forespores.the addition of 0.6 m sucrose of 0.016 m mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees c) in stage v bacillus cereus forspores. the addition of mg2+ to the sporulation medium did not remove this requirment for sucrose of mg2+. the heat damage did not affect forespore germination or outgrowth, but injured cells in the absence of sucrose or mg2+ were not capable of cell division. the heat-induced sublethal damage apparently affected the f ...1976823870
morphological changes in escherichia coli and bacillus cereus caused by external histone.it was found that the externally added histone changes remarkably both the surface and the internal ultrastructure of cells of escherichia coli. the interaction of histone with surface structures results in thickening of the inner layer of the cell wall. cytoplasm becomes condensed, contains extensive electrontransport zones and neither ribosomes nor the nuclear structure are differentiated. the addition of histone to germinating spores of bacillus cereus decelerates germination and postgerminat ...1976824179
identification of a novel enterotoxigenic activity associated with bacillus cereus.a strain of bacillus cereus isolated from a food poisoning outbreak characterized by vomiting has been shown to be capable of causing vomiting when cultures grown on rice, but not other media, were fed to rhesus monkeys. in contrast, a strain isolated from a diarrhoeal outbreak produced diarrhoea, but not vomiting, when grown on various media in similar feeding trials. furthermore, culture filtrates from the diarrhoeal strain caused fluid accumulation in ligated rabbit ileal loops whereas those ...1976824324
studies on the production of enterotoxins by bacillus cereus.evidence is presented for the existence of three distinct enterotoxins detected in concentrated cell-free culture filtrates of selected bacillus cereus strains. the first was a product capable of stimulating the adenylate cyclase-cyclic-amp system in intestinal epithelial cells and, possibly through this, causing fluid accumulation in ligated ileal sections ("loops") of young rabbits. this was elaborated by a strain isolated from an incident of diarrhoea and which caused diarrhoea in 6 of 10 mon ...1976824325
effect of nisin on the germination and proliferation of spores of some aerobic spore-formers in nutrient medium. 1976824886
a rapid method for the purification of beta-lactamase from bacillus cereus by affinity chromatography. 1976824973
food poisoning outbreak attributed to bacillus cereus. 1976825214
the structure of phosphatidyl glycerol prepared by phospholipase d-catalyzed transphosphatidylation from egg lecithin and glycerol. 1976825236
highly sensitive determination of bacillus cereus exo-enterotoxin using the method of aggregate haemagglutination.the possibility has been demonstrated of using the method of aggregate-haemagglutination for the detection of b. cereus exo-enterotoxin in both food products and culture media. it has been established that 0.004 mug/ml of enterotoxin can be detected by this method. the applied antisera to b. cereus enterotoxin did not yield cross reactions with enterotoxins produced by e. coli, cl. perfringens, st. aureus, v. cholerae or sh. dysenteriae.1976825572
protection of rats by phospholipase c from bacillus cereus against the effects of intravenous infusions of purified tissue thromboplastin.infusions of purified tissue thromboplastin in rats cause the accumulation of fibrin and platelets in the lungs and produce marked changes in the platelet count and in the coagulation factors v, vii and viii. tissue thromboplastin in a dose corresponding to less than 2 mug of protein per rat is lethal when given as a bolus injection. simultaneous i.v. administration purified phospholipase c effectively prevents all these changes and protects rats from otherwise lethal doses of tissue thromboplas ...1976825958
the folate-mediated synthesis of ribothymidylate in transfer ribonucleic acid, and evidence for multifunctional enzymes in one-carbon metabolism in eukaryotic sources. 1976826434
biosynthesis of ribothymidine in the transfer rna of streptococcus faecalis and bacillus subtilis. a methylation of rna involving 5,10-methylenetetrahydrofolate.the methyl moiety of ribothymidine in the trna of streptococcus faecalis, bacillus subtilis, and probably bacillus cereus is dervied from a 1-carbon folate derivative and not s-adenosylmethionine. micrococcus luteus (m. lysodeikticus) trna appears to be almost devoid of ribothymidine. s. faecalis trna lacking ribothymidine can be methylated in vitro with formation of ribothymidine. 5,10-methylenetetrahydrofolate has been identified as the 1-carbon donor involved in ribothymidine formation in s. ...1976826533
[physicochemical characteristics of dna and the amino acid composition of bacillus phages].from five bacillus phages nucleic acids were isolated and identified as double-stranded dna of the at-type. their melting point was 85-86.5 degrees c, molecular weight 23.4-43.7x10(6) daltons. the content of dna in phage particles was 39.9-44.0%, the content of protein 55.7-56.1%. the examination of the amino acid composition in phages 125/50 and 617 revealed a low level of serine and the lack of methionine, while the remaining amino acid composition of bacillus phages was close to that of e. co ...1976827122
effect of near-ultraviolet radiation on growth and respiration of bacillus cereus t. 1976827494
the total structure of cerexin a (studies on antibiotics from the genus bacillus. xvi.the constituent fatty acid of cerexin a was elucidated to be beta-hydroxy isoundecanoic acid by gas chromatography, mass spectrometry and nuclear magnetic resonance. the asparaginyl asparagine linkage in the amino acid sequence of the antibiotic, which has been previously described, was proved to be a normal alpha-carboxylpeptide bond by dehydration-reduction procedure. the three asparagine residues in the sequence were isolated separately and their chiralities were determined. the structure of ...1976827539
the structure of cerexin b (studies on antibiotics from the genus bacillus. xvii).the structure of cerexin b was examined. the constitutent fatty acids were elucidated by gas chromatography and mass spectrometry to be beta-hydroxy isodecanoic acid, beta-hydroxy decanoic acid, beta-hydroxy isoundecanoic acid and beta-hydroxy anteisoundecanoic acid. the configurations of constituent amino acids were determined as asparagine (2d, 1l), valine (d), phenylalanine (d), allo-threonine (d), tryptophan (d), and allo-isoleucine (d) from their optical activities. treatment with conc. hyd ...1976827540
production and isolation of cerexins c and d studies on antibiotics from the genus bacillus. xvii.from the culture broth of bacillus cereus 60-6, in which antibiotic production was improved a new antibiotic, named cerexin c, was isolated. similarly, a new antibiotic, cerexin d was isolated from the culture broth of bacillus cereus gp-3. cerexins c and d are closely related to cerexins a and b in their physico-chemical and antimicrobial properties. in fact cerexins c and d are peptides essentially identical with cerexins a and b except for the presence of a lysine residue in place of the gamm ...1976827541
highly sensitive determination of bacillus cereus exo - enterotoxin using the method of aggregate haemagglutination.the possibility has been demonstrated of using the method of aggregate-haemagglutination for the detection of b. cereus exo-enterotoxin in both food products and culture media. it has been established that 0.004 mug/ml of enterotoxin can be detected by this method. the applied antisera to b. cereus enterotoxin did not yield cross reactions with enterotoxins produced by e. coli, cl. perfringens, st. aureus, v. cholerae or sh. dysenteriae.1976827583
[the effects of weak magnetic fields on bacteria].a stationary device was constructed for conducting experiments in a weak magnetic field. a ferromagnetic screen was used to produce in the working volume a magnetic-field of 26 nt. the effect of a weak magnetic field on the following bacteria was studied: e. coli communior, b. prodigiosum, staphylococcus aureus 209, and bac. anthracoides. cultivation of the bacteria in these conditions for a long time resulted in changes of their tinctorial, morphological, cultural, and biochemical properties.1976827667
[evaluation of successful chemotherapie of infections of the urinary system by determination of antibacterial activity in the urine (author's transl)].5,589 urin samples from patients with suspected infections of the urinary tract were screened for inhibiting activity against baz. cereus (atcc 6051). in 19% of these urines such inhibitors could be found. in spite of the antibacterial inhibition activity nearly half of the samples had bacteria in high number. by comparison of the various bacterial species in both groups of urin, in those without antibacterial "problem-bacteria": klebsiella-enterobacter- proteus- candida-species is demonstrated. ...1976828144
effect of phospholipase c hydrolysis of membrane phospholipids on acyltransferase systems in rat liver microsomes.three kinds of phospholipase c [ec 3.1.4.3] were used to selectively hydrolyze phospholipids in rat liver microsomes, and their effects on the acyl-coa: glycerophosphate and acyl-coa: lysophospholipids acyltransferase systems were examined. the glycerophosphate acyltransferase [ec 2.3.1.15] system was inactivated rapidly by treatment with phospholipase c of ps. aureofaciens or b. cereus and the loss of activity paralleled the degradation of phosphatidylcholine and phosphatidylethanolamine. the 1 ...1976828160
filamentous appendages of bacillus cereus spores. 1976828210
studies on megacinogeny in bacillus cereus. i. multiplication of phage wx causing lysogenic conversion to megacin a (phospholipase a) production.phage wx capable of reconverting bacillus cereus strain w derivatives, cured to lose megacin a (phospholipase a) production into megacin a-producing cultures, exhibits unusual kinetics of multiplication; its clear mutant, phage wxc, behaves similarly. the phages are not adsorbed by stationary phase indicator bacteria. as sonicated bacteria fail to inactivate the phages, the absence of adsorption cannot be attributed to an undersurface localization of the receptors. multiplying bacteria exert a s ...1976828440
studies on megacinogeny in bacillus cereus. ii. bacillus cereus isolates characterized by prophage-controlled production of megacin a (phospholipase a).five out of a number of bacillus cereus strains isolated from soil produced high titre specific bacteriocin (megacin a) in mitomycin c-induced cultures. in the course of cultivation with ethidium bromide, the strains gave off segregants not producing bacteriocin (cin-). the lysate of two wild strains formed plaques on the corresponding cin- bacteria. the two phages (wx23 and wx26) were identical in antigenic structure with phage wx was present in the lysate of b. cereus strain w, and converted c ...1976828441
the applications of immobilized enzymes in automated analysis. 1976828702
purification & properties of isozymes of glutamate dehydrogenase form bacillus cereus t. 1976828900
comparison of two methods for detecting antibiotic residues in slaughter animals.a comparison was made of the sensitivity of two methods in current use for the detection of antibiotic residues in slaughter animals. calves previously given therapeutic injections of an antibiotic were used and a variety of samples were tested.1976828970
inhibition of n5-methyltetrahydrofolate - homocysteine transmethylase by a vitamin b12-antimetabolite. 1976829218
[possible relationship between bacterial surface structures, immune-adherence, chemotaxis and phagocytosis (author's transl)].in the present study we attempted to determine the relationship between bacterial surface structures, immune adherence, chemotaxis and phagocytosis. as shown in table 1 encapsulated bacteria (klebsiella ozanae, k. pneumoniae, pasteurella multocida, streptococcus pneumoniae), mycoplasma pneumoniae and m. fermentans had a much lower immune adherence-activity than non-encapsulated bacteria (bacillus cereus, brucella abortus, erysipelothrix insidiosa, escherichia coli, listeria monocytogenes, pseudo ...19761258564
phosphatidyl-(n-acyl)-ethanolamine. a lipid component of mammalian epidermis.a lipid present in the granular cells of mammalian epidermis was identified as phosphatidyl-(n-acyl)-ethanolamine. the structure was deduced from the ratio of phosphorus : nitrogen : glycerol : fatty acid esters : total fatty acid (1 : 0.94 : 0.97 : 2.1 : 2.9), from analyses of the products of alkaline and acid hydrolyses and from its infrared spectrum. conclusive evidence was obtained by a direct comparison of the chromatographic properties, degradation products and infrared spectrum of the iso ...19761268235
bacteriophage and bacteriophage-like structures carried by bacillus medusa and their effect on sporulation.bacillus medusa was found to carry three phages or phagelike structures named phi med-1, phi med-2, and phi med-3. phi med-1 is a minute, 25-nm-diameter particle without a tail. it was extracted from the sporulation lysate of a phi med-2-minus strain of b. medusa and purified by differential centrifugation. the nucleic acid from this structure was shown to be orcinol positive, alkali sensitive, rnase sensitive, and dnase resistant. an rnase-resistant core of nucleic acid was not found, indicatin ...19761271529
food poisoning. 1976815943
[normal status of bac. cereus and cl. perfringens in the body of healthy slaughter animals].studied were cattle, pigs, sheep, and young calves intended for slaughter. the experiments were carried out under productional conditions, strictly observing the routinely adopted practice of preslaughter handling. the blood of the animals was sampled prior to slaughter. samples from the meat (musculature), spleen, kidneys, liver, mesenterial and body lymph nodes as well as feces were taken immediately after slaughter. it was established that bacillus cereus and clostridium perfringens were almo ...1976816051
studies on two isozymes of aconitase from bacillus cereus t. ii. further evidence on two distinct activities. 1976820347
biochemical effects in bacillus subtilis after treatment with the mycotoxin patulin. 1976184606
laboratory diagnosis of foodborne diseases.many bacterial species are responsible for sporadic cases and outbreaks of foodborne intoxication and infection. the foodborne diseases are classified on the basis of the pathogenetic mechanisms involved into four categories: performed toxin, enterotoxin formed in the colonized small intestine, mucosal invasion (enterocolitis) and mucosal invasion with bacteremia. invasive and toxigenic strains of enteropathogenic escherichia coli are discussed. in vivo test systems for the identification of ent ...1976184732
selective utilization of endogenous unsaturated phosphatidylcholines and diacylglycerols by cholinephosphotransferase of mouse lung microsomes.in the presence of cmp, cholinephosphotransferase of mouse lung microsomes catalyzes the conversion of endogenous phosphatidylcholines into 1,2-diacyl-sn-glycerols and cdpcholine. 2. in this conversion cholinephosphotransferase shows a distinct preference for those molecular species of phosphatidylcholine which contain an unsaturated fatty acid. the enzyme hardly utilizes endogenous depalmitoylglycerophosphocholine as a substrate. 3. membrane-bound 1,2-diacyl-sn-glycerols were also prepared by t ...1976184825
fine structure of the baccilus thuringiensis spore.the thin-sectioned spore of bacillus thuringiensis resembles that of bacillus cereus in fine structure. planar inclusions occur between the exosporium and spore coat and are structured differently from the parasporal crystal outside the exosporium.1976185959
the effect of phospholipase c on dna synthesis, morphology and phospholipid content of isolated hela cell nuclei.isolated hela cell nuclei have been treated with purified phospholipase c (bacillus cereus) and sphingomyelinase (staphylococcus aureus). the phospholipids of untreated nuclei consisted of about 67% phosphatidylcholine, 23% phosphatidylethanolamine, 7% sphingomyelin, 2% phosphatidylserine and 1% phosphatidylinositol. phospholipase c degraded 80-90% of the total phospholipids of the nuclei. such nuclei seemed ultrastructurally intact, and had an average diameter and a protein loss during incubati ...1976187233
gonadotropin receptors in plasma membranes of bovine corpus luteum. i. effect of phospholipases on the binding of 125i-choriogonadotropin by membrane-associated and solubilized receptors.the ability of bovine corpus luteum plasma membranes to bind 125i-choriogonadotropin has been examined after prior treatment of the membranes with phospholipases a, c, and d. treatment of the purified membranes with low concentrations of phospholipases a and c resulted in the inhibition of the binding of 125i-choriogonadotropin to its receptors, whereas phospholipase d had no effect. receptor activity was decreased by low concentrations of phospholipase a from either bee venom, vipera russelli o ...1976187585
[microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses].the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ...1976190825
effect of delayed evisceration on the microbial quality of meat.the postomortem invasion of muscle and other tissues by bacteria from the intestinal tract was studied with the use of radioactive tracers. the injection of 14c-labeled bacteria or spores into the intestines of guinea pig carcasses within 24 h of death resulted in the rapid spread of 14c throughout carcasses. when live bacteria were injected along with the labeled cells, it was not possible to isolate viable organisms from the body tissues if the living animal had been exposed to the bacteria. i ...1976178276
bacillus cereus food poisoning. 1976179147
[characterization by zymograms of r factor-determined beta-lactamases: plasmids of defined and undefined compatibility groups (author's transl)].an easy biochemical method is described, discriminating the beta-lacta-mases coded by various r factors. electrophoretic mobility and substrate affinity of beta-lactamases were studied simultaneously for 7 different penicillins and cephalosporins. they are i like r factors assigned into different compatibility groups (n, p, com6, com7, com8, x and com 9), five r factors isolated from piglets faeces and one r factor, ru1, originated from enterobacter aerogenes. three electrophoretic types of beta ...1976786104
[bacterial flora of the conjunctival sac of the horse].the aa. report the results of taxonomic research conducted on the conjunctival sac of 59 horses for identification of the present bacterial flora. in the controlled animals, it was observed, at the level of the considered niche, a community constituted of normal bacterial populations, but not autochtonous in the significance they attributed from dubos et al., relative to the characterization of the indigenous microbiota of the intestine. the isolated normal bacterial flora seems to be constitute ...1976788654
effects of phenethyl alcohol on bacillus and streptococcus.the activity of phenethyl alcohol (pea) on bacillus cereus, b. megaterium, and streptococcus faecalis was studied by electron microscopy of thin sections and by the assay of intracellular k+ leakage. s. faecalis was unaffected by pea at concentrations up to 0.5%, b. cereus was severely damaged by 0.5% pea, and b. megaterium behaved intermediately. important membrane ultrastructural alterations were observed in b. cereus cells treated with 0.5% pea, namely the change in the geometry of the membra ...197660333
rational choice of penicillins and cephalosporins based on parallel in-vitro and in-vivo tests.because of the unavailability of strictly comparable data, seven representative penicillins and the five cephalosporins currently used in britain were evaluated in parallel, both in vitro and in vivo. penicillin sensitive and resistant strains of staphylococcus aureus and proteus mirabilis were the main test organisms. minimum bacteriocidal concentrations of cloxacillin, flucloxacillin, cephalothin, and cephazolin in serum were much higher than conventional minimum inhibitory concentrations in t ...197661353
phospholipase of bacillus cereus.phospholipase activity of 10 strains of bacillus cereus was studied. the most active strain of bac. cereus--phospholipase producer was selected. a cultivation mixture of bac. cereus optimal for the phospholipase synthesis was found to include peptone, yeast extract, glucose, nacl and na2hpo4. proper conditions for the synthesis of phospholipase in flasks, 20 l and 250 l fermenters were tested. the maximum increase of the phospholipase activity occurred by the 5-9th hour of microbial growth at ph ...197617109
studies on two isozymes of aconitase from bacillus cereus t. iii. enzymatic properties. 19767257
the occurrence of bacillus cereus in finnish dog food sausages; a microbiological and physiochemical survey.a bacteriological survey of five brands of commercial dog food sausages showed that two of them were heavily contaminated with b. cereus. one of the two brands had a suspected association with food-poisoning incidents in dogs. in addition to b. cereus the other sausage brand was found to contain a high number of sulphite reducing anaerobes. b. cereus was detected in almost every raw material of the two brands of sausages. the pasteurization during manufacturing did not kill all the sporeforming ...19767770
influence of growth temperature on glucose metabolism of a psychotrophic strain of bacillus cereus.the influence of temperature on glucose metabolism of a psychotrophic strain of bacillus cereus was investigated. the ph of the growth medium and spore-forming frequencies of b. cereus varied when grown at 32, 20, or 7 c. radiorespirometric analyses revealed that vegetative cells of b. cereus metabolized glucose by simultaneous operation of the embden-meyerhof-parnas pathway and the pentose phosphate pathway. as the growth temperature decreased, glucose was metabolized with increased participati ...19768003
Displaying items 6601 - 6700 of 8187