Publications

inhibition by ammonium ion of germination of unactivated spores of bacillus cereus t induced by l-alanine and inosine.	studies were carried out on the inhibitory effect of nh4+ on germination of spores of bacillus cereus t induced by l-alanine and inosine. kinetic analysis showed that nh4+ inhibited the germination competitively. its inhibitory effect was greater when the unactivated spores had been preincubated with l-alanine. nh4+ did not inhibit the response of unactivated spores to l-alanine during preincubation. these results suggest that l-alanine sensitizes the spores to the inhibitory effect of nh4+.	1978	97499
bacillus cereus endocarditis. a case report.	bacillus cereus may cause infective problems in compromised patients. no previous record of infective endocarditis due to this organism could be found. a 51-year-old white woman with b. cereus endocarditis after prosthetic mitral valve replacement is described. the problems of interpreting the significance of b. cereus bacteraemia, delayed diagnosis, and the inherent resistance of the organism are discussed.	1978	97794
[study of lysogeny in bacillus thuringiensis and b. cereus].	forty-eight strains of bacillus thuringiensis and 12 strains of b. cereus were treated with ultraviolet light and mitomycin c. the former agent was the more effective inducer. bacillus thuringiensis produces at least seven different phage particles with long, non-contractile tails. the frequencies of lysogeny and polylysogeny are 83 and 25% respectively. morphologically defective phages occur in 25% of strains, whereas five of them produce low molecular-weight bacteriocins. one strain of b. cere ...	1978	98223
effects of phenylglyoxal on growth & sporulation of bacillus cereus t.		1978	98431
formation and function of n-acetyloglucosamine-linked phosphoryl- and pyrophosphorylundecaprenols in membranes from bacillus cereus.	membranes from bacillus cereus ahu 1356 incorporated radioactivity from udp-n-acetyl[14c]glucosamine into three alkaline-stable and acid-labile lipids which were extracted into chloroform:methanol (2:1) and separated from each other by thin layer chromatography on silica gel plates. the major labeled lipid (lipid 1) and a minor one (lipid 2) were identified as n-actetylglucosaminyl phosphorylundecaprenol from several analytical criteria involving mass spectral data and from reversal of their for ...	1978	98528
two purine nucleoside phosphorylases in bacillus subtilis. purification and some properties of the adenosine-specific phosphorylase.	two purine nucleoside phosphorylases (purine-nucleoside:orthophosphate ribosyltransferase, ec 2.4.2.1) were purified from vegetative bacillus subtilis cells. one enzyme, inosine-guanosine phosphorylase, showed great similarity to the homologous enzyme of bacillus cereus. it appeared to be a tetramer of molecular weight 95 000. the other enzyme, adenosine phosphorylase, was specific for adenosine and deoxyadenosine. the molecular weight of the native enzyme was 153 000 +/- 10% and the molecular w ...	1978	99174
[method of determining the antimicrobial activity of alcohol extracts of propolis].	it is proposed to determine the antimicrobial activity of propolys alcohol extracts by the method of subsequent dilutions in solid nutrient media. dilution of the extracts immediately in hot agar eliminated the inhibiting effect of the extragent on the microbial growth. opalesence appearing in the agar did not prevent estimation of the results in contrast to the method of subsequent dilutions in liquid nutrient media.	1978	100047
delay in growth induced by glass filtered solar radiation in bacillus cereus.		1978	100420
utilization of exogenous purine compounds in bacillus cereus. translocation of the ribose moiety of inosine.	intact cells of bacillus cereus catalyze the breakdown of exogenous amp to hypoxanthine and ribose 1-phosphate through the successive action of 5'-nucleotidase, adenosine deaminase, and inosine phosphorylase. inosine hydrolase was not detectable, even in crude extracts. inosine phosphorylase causes a "translocation" of the ribose moiety (as ribose 1-phosphate) inside the cell, while hypoxanthine remains external. even though the equilibrium of the phosphorolytic reaction favors nucleoside synthe ...	1978	100497
effect of hydrostatic tensile stress on the growth of escherichia coli and bacillus cereus.	the specific growth rates of escherichia coli and bacillus cereus were measured for growth media in a flask, a lens-plate arrangement simulating an isolated capillary space, and a lens-plate arrangement under hydrostatic tensile stress. the specific growth rates of the bacteria were the same for the flask and lens-plate arrangement without hydrostatic tensile stress, but were enhanced when the growth media were subjected to hydrostatic tensile stress. the enhanced specific growth rates reached s ...	1978	101141
studies on phosphatidylinositol phosphodiesterase (phospholipase c type) of bacillus cereus. ii. in vivo and immunochemical studies of phosphatase-releasing activity.		1978	101146
isolation of stable ribosomal subunits from spores of bacillus cereus.	analyses of ribosomes extracted from spores of bacillus cereus t by a dryspore disruption technique indicated that previously reported defects in ribosomes from spores may arise during the ribosome extraction process. the population of ribosomes from spores is shown to cotain a variable quantity of free 50s subunits which are unstable, giving rise to slowly sedimenting particles in low-mg2+ sucrose gradients and showing extremely low activity in in vitro protein synthesis. the majority of the ri ...	1978	101512
anti-riboflavin activity of 8n-alkyl analogues of roseoflavin in some gram-positive bacteria.	three new 8n-alkyl analogues of roseoflavin (mm), i.e., 8-ethylamino- (eh), 8-methylethylamino- (me), 8-diethylamino-8-demethyl-d-riboflavin (ee), their tetraacetates, and 8-amino-8-demethyl-d-riboflavin (hh) tetraacetate, were synthesized. a relation between the anti-riboflavin activity and the chemical structure of 8n-alkyl analogues (8n-methyl, ethyl) was studied by a restoration by riboflavin (rf) of inhibitory effect of the analogues on a growth of gram-positive bacteria, i.e., sarcina lute ...	1978	101644
endogenous endophthalmitis associated with bacillus cereus bacteremia in a cocaine addict.	a 22-year-old black female intravenous cocaine addict presented with an endophthalmitis of the right eye. diagnostic evaluation included an immediate anterior chamber paracentesis and a delayed vitreous aspiration. although cultures from the involved eye were negative, all 7 blood cultures grew bacillus cereus suggesting that this organism was the responsible agent of an endogenous endophthalmitis. the patient was treated with appropriate systemic and local antibiotics with resolution of the acu ...	1978	102235
role of cholesterol in the action of cereolysin on membranes.		1978	102256
semi-automated detection of emesis in the rhesus monkey.	the use of a video recorder and the inclusion of a marker dye in material fed to rhesus monkeys has eliminated the need for the constant presence of an observer to detect an emetic response.	1978	102332
accessibility of phospholipids in the chromaffin granule membrane.	1. the accessibility of phospholipids in the membrane of the adrenomedullary storage vesicles (chromaffin granules) has been studied. 2. the reaction of 2,4,6-trinitrobenzenesulphonic acid with both intact granules and their ghosts, results in the labelling of 70% of the phosphatidylethanolamine. 3. the action of phospholipase a2 (from bee venom), phospholipase c (from bacillus cereus) and sphingomyelinase c (from staphylococcus aureus) on granules and their ghosts was followed as a function of ...	1978	102348
clavulanic acid inhibition of beta-lactamase i from bacillus cereus 569/h.	inactivation of beta-lactamase i by clavulanic acid was investigated. clavulanic acid induced inhibition of the enzyme was found to be progressive with time. benzylpenicillin provided protection against the adverse effects of the inhibitor initially, however, the enzyme was irreversibly inhibited in a progressive manner even in the presence of substrate. reaction of beta-lactamase i with clavulanic acid, in the presence of ampicillin, led to a very rapid inactivation of the enzyme.	1978	102630
inhibition by potassium ion of the pregerminative response to l-alanine of unactivated spores of bacillus cereus t.	the effect of potassium ion on l-alanine-inosine-induced germination of unactivated spores of bacillus cereus t was studied. unactivated spores germinated in 0.1 m sodium phosphate buffer (napb), but not 0.1 m potassium phosphate buffer (kpb), at ph 8.0 and at 30 c. inhibition of germination was also observed on incubation of unactivated spores in napb containing potassium chloride. previously it was demonstrated that germination of unactivated spores involves at least two steps, one induced by ...	1978	102904
[supplementary approach to the differentiation of the spore bacteria, bacillus subtilis and bacillus cereus].		1978	102905
case report: bacillus cereus food poisoning.		1978	103816
relationship of bacillus subtilis dna polymerase iii to bacteriophage pbs2-induced dna polymerase and to the replication of uracil-containing dna.	in vivo studies of pbs2 phage replication in a temperature-sensitive bacillus subtilis dna polymerase iii (pol iii) mutant and a temperature-resistant revertant of this mutant have suggested the possible involvement of pol iii in pbs2 dna synthesis. previous results with 6-(p-hydroxyphenylazo)-uracil (hpura), a specific inhibitor of pol iii and dna replication in uninfected cells, suggest that pol iii is not involved in phage dna replication, due to its resistance to this drug. experiments were ...	1978	104052
[enteropathogenicity of bacillus cereus strains].		1978	104365
[effect of a bacillus cereus 504 culture on the development of homologous strain cells].		1978	416328
determination of fatty acid compositions of bacillus cereus and related bacteria: a rapid gas chromatographic method using a glass capillary column.	a rapid gas chromatographic method for the determination of fatty acid compositions of bacillus cereus and related bacteria is presented. by the use of a free fatty acid phase-coated glass capillary column, the complete separation of fatty acids, including the branched ones, was achieved. the method enables a more distinct differentiation of bacillus species than can be obtained with packed columns.	1978	416751
fatty acids of gram-positive bacterial rods from human dental plaque.		1978	416814
[radiosensitivity of the bacteriophages of aerobic sporulating microorganisms].		1978	417102
low temperature microspectrofluorometry: design of a 'cold chamber'.	low temperature microspectrofluorometry allows an improvement of spectral resolution and an increase of fluorescence intensity. suppression of fluorescence fading, or at least a marked reduction, is also obtained. a cooling chamber for microspectrofluorometric measurement is described which allows the cooling, under a microscope, of a biological sample down to liquid nitrogen temperature. objectives with numerical apertures better than 1.0 and a magnification power up to 100x can be used. low te ...	1978	417183
osmotically induced increase in thermal resistance of heat-sensitive, dipicolinic acid-less spores of bacillus cereus ht-8.	thermal resistance in heat-sensitive, dipicolinic acid (dpa)-less spores of bacillus cereus ht-8 heated in sucrose solutions increased at and above a concentration of 2 m sucrose. the decimal reduction times at 75 degrees c for spores heated in 0.0, 1.8, 2.2, and 2.6 m sucrose were 2.0, 2.8, 4.5, and 12 min, respectively. maltose, fructose, and glucose increased heat resistance above that observed in water but did not elevate resistance to the level observed with sucrose at the same osmolality. ...	1978	417675
enzymatic formation of uridine diphosphate n-acetyl-d-mannosamine.	an enzyme that catalyzes the interconversion of udp-n-acetyl-d-glucosamine and udp-n-acetyl-d-mannosamine was purified about 700-fold from the supernatant fraction of bacillus cereus, and the properties of this enzyme were studied. this enzyme was not stimulated by nad+, nadh, or any metal ions. the optimum ph was between 7.5 and 8.0. at equilibrium of the reaction, the ratio of udp-n-acetylglucosamine to udp-n-acetylmannosmaine was about 9:1. the enzyme was inactive toward free n-acetylhexosami ...	1978	418068
carboxymethylcellulase produced by facultative bacteria from the hind-gut of the termite reticulitermes hesperus.	bacillus cereus rw1 and serratia marcescens rw3, isolated from the hind-gut of the termite reticulitermes hesperus, both grew well on mesquite wood and produced moderate amounts of carboxymethylcellulase. carboxymethylcellulose (cmc) gels were depolymerized rapidly by b. cereus rw1 and slowly by s. marcescens rw3. the depolymerization of cmc was ph and temperature sensitive. depolymerization of gels by growing cultures of b. cereus rw1 and the action of cell-free extracts of b. cereus rw1 on cmc ...	1978	418144
preliminary crystallographic data for beta-lactamase i from bacillus cereus 569.		1978	418181
[comparative investigations on the efficiency of chlorine and ozone against bacteria and spores (author's transl)].	this paper is concerned with the measurement of first order rate constants for the death of bacteria by chlorine and ozone. while the first order conditions for chlorine were achieved with the aid of a new instrument which works basing on the polarographic principle this was attained for ozone under steady state conditions. besides differences of species some different properties for both oxidants were found as far as their bactericidal efficiency is concerned. they are discussed in this paper.	1978	418606
[serological properties of species of the genus bacillus].		1978	82901
cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis.	the spin-labeled cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phosphol]-sn-glycerol has been prepared. the stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase a2 (phosphatide 2-acylhydrolase, ec 3.1.1.4) of trimeresurus flavoviridis. the results of treatment of the spin-labeled cardiolipin with the cardiolip ...	1978	274715
bacillus cereus food poisoning.		1978	413036
alteration by cereolysin of the structure of cholesterol-containing membranes.	when erythrocyte membranes were treated with cereolysin, negatively stained and examined by electron microscopy, ring and arc-shaped structures were observed in the membrane. the outside diameter of the rings varied from 33 to 50 nm with a border thickness of 6.7 to 8.3 nm. the arcs varied in length from 33 to 170 nm with a border thickness of also 6.7 to 8.3 min. when right-side-out erythrocyte ghosts which had been treated with cereolysin were examined by electron microscopy after freeze-fract ...	1978	414780
spore location patterns in sporulating doublets of bacillus cereus and bacillus megaterium, derived from single doublet isolates with differing sporulation geometry.	the distribution of spore loci in pairs of bacillus cereus and b. megaterium showed different degrees of polarization from random location towards location at the old end of the sporangium. when individual doublets containing both spores at either the old or new ends of the sporangia were isolated by micromanipulation, subsequent culture showed the same spore location patterns.	1978	415036
membrane-bound nucleotidase of bacillus cereus.	a membrane-bound nucleotidase of bacillus cereus t was solubilized by digestion with trypsin and subsequently purified more than 300-fold. the purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. in addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to ...	1978	415044
bacteriocin and antibiotic resistance plasmids in bacillus cereus and bacillus subtilis.	a number of plasmids have been isolated as covalently closed circular dnas from strains of bacillus cereus and b. subtilis. from 12 out of 15 strains of b. cereus, plasmids could be isolated. most of the b. cereus strains contained two or more plasmids. their molecular weights ranged from 1.6 x 10(6) to 105 x 10(6). bacteriocin production could be attributed to a 45 x 10(6)-dalton plasmid (pbc7) from b. cereus dsm 336, and tetracycline resistance to a 2.8 x 10(6) plasmid (pbc16) from b. cereus g ...	1978	415051
reversible inhibitors of penicillinases.	reversible competitive inhibitors of a penicillinase, beta-lactamase 1 from bacillus cereus, were studied. these represent the first inhibitors of a penicillinase that lack the beta-lactam ring. the products of the enzymic reaction, namely penicilloic acids, are inhibitors; their decarboxylation products, the penilloic acids, are also inhibitors, and have somewhat lower ki values. inhibitors have been prepared from benzylpenicillin, phenoxymethyl-penicillin, methicillin (2,6-dimethoxybenzamidope ...	1978	415738
induction of penicillinase by bacitracin.		1978	415741
prevalence of bacillus cereus in the faeces of healthy adults.	in a survey designed to determine the prevalence of bacillus cereus in the faeces of healthy persons, the organism was found in low numbers in 100 (14%) of single faecal specimens from 711 adults in the general population. in addition, in an attempt at assessing the changes in the b. cereus distribution within the faecal flora of the individual, weekly faecal specimens were submitted over a seven-week period by 18 members of staff of two laboratories. the total isolation rate was again 14%, with ...	1978	416141
characterization of a bacillus cereus protease mutant defective in an early stage of spore germination.	temperature-sensitive sporulation mutants of bacillus cereus were screened for intracellular protease activity that was more heat labile than that of the parental strain. one mutant grew as well as the wild type at 30 and 37 degrees c but sporulated poorly at 37 degrees c in an enriched or minimal medium. these spores germinated very slowly in response to alanine plus adenosine or calcium dipicolinate. during germination, spores produced by the mutant rapidly became heat sensitive, but released ...	1978	412836
the role of various milk fractions and the importance of somatic cells in the formation of germinant(s) for bacillus cereus when milk is pasteurized.		1977	412883
[a case of acute myelogenous leukemia, complicated with bacillus cereus septicemia (author's transl)].		1977	416149
[germination and outgrowth of spores and proliferation of outgrown vegetative cells in the peritoneal cavity of mice intraperitoneally injected with bacillus cereus or bacillus subtilis spores (author's transl)].		1977	415160
lipoquinones of some bacteria and mycoplasmas, with considerations on their functional significance.	in a comparative study the lipoquinones of some chemoorganotrophic, facultatively aerobic bacteria, and representative acholeplasma, mycoplasma, spiroplasma, and thermoplasma strains were investigated. the quinones were partly purified by preparative thin layer chromatography of lipid extracts, and characterized by their difference spectra (reduced minus oxidized) and rf values. respiring bacteria expectedly contained benzoquinones and/or naphthoquinones in micromolar concentrations whereas some ...	1977	413478
the histidine residues of phospholipase c from bacillus cereus.	the inactivation of phospholipase c from bacillus cereus at ph6 by diethyl pyrocarbonate parallelled the n-ethoxyformylation of a single histidine residue in the enzyme. the inactivation arose from a decrease in the maximum velocity of the enzymic reaction with no effect on the km value. the inactivation did not apparently alter the ability of the enzyme to bind to a substrate-based affinity gel. the native enzyme contained only one reactive histidine residue. removal of the two zinc atoms from ...	1977	413541
[admixtures of antibiotic substances in enzyme preparations of microbial origin intended for use as food additives].	a group of enzymtic preparations, mainly of the soviet make were investigated for the presence of antibiotic substances admixtures. altogether 25 preparations obtained from molds, actinomycetes, bacteria and yeast were studied. the results showed that 18 preparations contained antibiotic substances in an amount of 0.6-1300 u, equivalent to oxytetracycline units per 1 g. most often antibiotics were present in preparations of the pectinase and protease group. in the group of glucoamylase the antib ...	1977	414448
[clinico-epidemiologic characteristics of group illness caused by bac. cereus food poisoning].	the authors describe an outbreak of food poisoning caused by bac. cereus embracing 80% of persons who catered at the same catering establishment. the poisoning occurred in the course of 15 hours after the moment of eating a sauce stored in warm place. in the majority of cases the course of the disease was mild; 77.8% of the patients displayed phenomena of enteritis, 20.2%--of entercolitis, and 2.0%--of gastroenterocolitis. all the patients recovered without any treatment in the course of 1--2 da ...	1977	414486
membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts.	nascent calcium phosphate promotes the agglutination and fusion of human erythrocyte ghosts. membrane phospholipids of erythrocyte ghosts treated with ca2+ and phosphate ions become exposed to attack by phospholipase c (phosphatidylcholine cholinephosphohydrolase, ec 3.1.4.3) (bacillus cereus). freeze-fracture pictures of fused erythrocyte ghosts show the presence of regions deficient in intramemebrane particles in the protoplasmic face which we believe to be regions of fusion. discontinuous reg ...	1977	329283
[methods of demonstrating antibiotics residues in meat, organs and eggs].		1977	416557
[effect of substituted isatinisothiosemicarbazones on the multiplication of bacteria].		1977	416604
[on the utilization different organic and anorganic compounds by some strains of bac. cereus var. mycoides (flügge) (author's transl)].	in order to investigate the decomposition of 46 organic and anorganic compounds by bac. mycoides, the behaviour of four strains was tested by means of plate counting technique (after koch) and the warburg method. mostly intensive substances containing protein were decomposed by all strains. furthermore amino acid, sugar, alcohols, and ammonium salts are used by the bacteria, but there were large differences between strains tested. urea and nitrates have not been decomposed.	1977	416618
the growth and respiration of bacterial colonies.	young colonies of two swarming organisms, bacillus subtilis and proteus vulgaris, grew about as quickly on solid media as in liquid culture whilst four non-swarming organisms, bacillus cereus, enterobacter cloacae, escherichia coli and staphylococcus albus, all grew slower on solid than in liquid media. oxygen uptake by young colonies of b. subtilis, followed manometrically, increased exponentially at about the same rate as unrestricted aerobic growth. all other colonies demonstrated acceleratin ...	1977	591923
[some ultrastructural characteristics of the sporal envelops of bacillus cereus].		1977	99733
effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph.	fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ...	1977	18085
studies on the thiamine transport system in bacillus cereus (author's transl).	the thiamine transport system in bacillus cereus exhibits rhythmical changes of resorption- and excretion-phases lasting 1-2 h. these main phases are subdivided in shorter ones with an average duration of 45 s. the velocity of the thiamine uptake is influenced by ph, temperature, age of cells, energy and substrate supply and thiamine concentration of the medium. the michaelis-menten-kinetic can be used to describe the uptake: km = 1.98 x 10(-8) m; vmax = 1.19 x 10(-6) mol/g dry weight x min. the ...	1977	19001
purification of phospholipase c from bacillus cereus by chromatography on aminoalkylpolysaccharide adsorbents.	purification of phospholipase c from bac. cereus by chromatography on aminoalkylpolysaccharide adsorbents is described. the dependence of the degree of enzyme purification on the amount of ligant and effect of ph and buffer systems on the adsorption-desorption of phospholipase have been studied. at a ph below 9.0 phospholipase c is not retained by the adsorbents and is purified 4-5-fold and up to 23-fold, when aminoalkyl-sepharose and hexamethylenediamine sephadex are used respectively. with an ...	1977	19100
an invitro system for the biosynthesis of spore cortex peptidoglycan.		1977	19145
dihydrodipicolinate reductases from bacillus cereus and bacillus megaterium.	dyhydrodipicolinate reductases were purified 100-fold from crude extracts of b. cereus and b. megaterium and their properties were compared with those of the reductase from b. subtilis. the molecular weights of the reductases of b. cereus and b. megaterium were fount to be 155,000 and 150,000, respectively. these reductases were shown to be free of flavin, unlike the b. subtilis enzyme, which contains flavin. both nadph and nadh acted as coenzymes for these two reductases. nadph being three or f ...	1977	19431
two omega-amino acid transaminases from bacillus cereus.	bacillus cereus strain k-22 produced two distinct omega-amino acid transaminases, one catalyzing the transamination between beta-alanine and pyruvic acid and the other that between gamma-aminobutyric acid and alpha-ketoglutaric aic. the two enzymes were partially purified and separated from each other by various chromatographies. beta-alanine:pyruvic acid transaminase and gamma-aminobutyric acid:alpha-ketoglutaric acid transaminase were induced by the addition of beta-alanine and gamma-aminobuty ...	1977	19432
spectrophotometric determination of ribose-1-phosphate.		1977	20811
n-(2-furyl) acryloyl penicillin: a novel compound for the spectrophotometric assay of beta-lactamase i.		1977	22529
alterations of spore coat processing and protein turnover in a bacillus cereus mutant with a defective postexponential intracellular protease.	a mutant with an alteration in the major intracellular serine protease produced by postexponential bacillus cereus was isolated by screening mutants defective in spore germination. the purified enzyme from the mutant is more labile to heat and alkaline ph than the protease from the wild type. protease activity appears at the same time as in the wild type but only reaches 50% of the specific activity and decays more rapidly during sporulation. coincident with the decay is a decrease in the rate o ...	1977	15254
reversibility of the ampicillin-and nitrite-induced inactivation of beta-lactamase i.	beta-lactamase i was isolated from bacillus cereus 569/h. treatment with ampicillin in the presence of sodium nitrite at ph 4 or 5 resulted in the inactivation of the enzyme presumably by modification of a carboxyl group in the active site. however, this inactivation was rapidly, reversible at neutral ph and the available evidence points to the participation of a second carboxyl group which is involved in the reactivation process.	1977	15712
phospholipase c from bacillus cereus. action on some artificial lecithins.	the hydrolysis by phospholipase c from b. cereus of several lecithins of different fatty acyl chain length was examined. the enzyme showed significant activity towards mono-molecularly dispersed short chain lecithins and the reaction obeyed normal michaelis-menten kinetics. rate vs. substrate concentration curves obtained with dihexanoyl-, diheptanoyl- and dioctanoyllecithins showed marked discontinuities in the region of the known critical micelle concentrations for these substrates and distinc ...	1977	16420
understanding the actions of carcinostatic drugs to improve chemotherapy: 5-fluorouracil.		1977	99991
an unusual outbreak of food-poisoning associated with meals-on-wheels.	an outbreak of food-poisoning after a meals-on-wheels lunch affected 49 persons, 1 of whom died. bacillus cereus serotype 10 and bacillus licheniformis were isolated in large numbers from many of the patients including the deceased and from the remains of the meal. clostridium perfringens (c. welchii) serotype 68, which was isolated from many of the patients and the deceased but not from the food, may also have been responsible. food kept warm during distribution can produce an ideal environment ...	1977	69207
some characteristics of phospholipase c from bacillus cereus.		1977	72664
some properties of a cell-free cortisol 1,2-dehydrogenating system from bacillus cereus.		1977	121321
[new findings on various bacteria and their toxins as possible causative agents of food poisoning].		1977	203102
evidence for distinct guanine nucleotide sites in the regulation of the glucagon receptor and of adenylate cyclase activity.		1977	197077
the specificity of s1 nuclease toward rna-dna hybrids as studied using isotopes of phosphorus-32 and phosphorus-33.	hybrids were formed from bacillus cereus dna and ribosomal rna. they were treated with various combination of s1 nuclease and ribonuclease, and the molar ratios of the rna and dna moieties remaining in the treated hybrids were determined using a 32p-33p dual-label technique. it was found that both s1 nuclease and ribonuclease are required to give hybrid with rna and dna in a perfect 1:1 molar ratio. it was noted that the dual-label technique which employs orthophosphate as the sole phosphorus so ...	1977	197495
bacterial hazards.		1977	197534
theta-toxin of clostridium perfringens. i. purification and some properties.	theta-toxin, an oxygen-labile hemolysin produced by clostridium perfringens, was purified 3300 fold from culture filtrate by successive chromatography on deae-sephadex a-50 and sephadex g-150. the purified toxin gave two distinct bands in disc electrophoresis, while the same material, after mild reduction with dithiothreitol, yielded a single band, indicating that the purified theta-toxin contained, as well as a reduced, active form, an oxidized, inactive form of toxin. these two forms of the to ...	1977	199270
aldolase-like imine formation in the mechanism of action of phosphonoacetaldehyde hydrolase.	phosphonoacetaldehyde hydrolase (ec 3.11.1.1), the bacterial enzyme that catalyses the reaction hco-ch2-po(oh)2+h2o leads to hco-ch3+pi, is inactivated by borohydride if either phosphonoacetaldehyde or acetaldehyde is present. this supports the suggestion that the substrate forms an imine with an amino group of the enzyme. such imine formation would labilize the c-p bond in the same way that aldolase and related enzymes labilize c-c and c-h bonds (scheme 1a).	1977	200222
hydrolytic action of phospholipases on bacterial membranes.	substrate specificities of phospholipases c[ec 3.1.4.3] from clostridium novyi, clostridium perfringens, bacillus cereus, and pseudomonas aureofaciens were studied under the same conditions. phospholipases c from clostridium novyi and bacillus cereus show wide substrate specificities while those of clostridium perfringens and pseudomonas aureofaciens show relatively narrow specificities. on the basis of these results, the hydrolytic actions of these phospholipases on membrane lipids of escherich ...	1977	201610
the lipid requirement of the (ca2+ + mg2+)-atpase in the human erythrocyte membrane, as studied by various highly purified phospholipases.	1. when complete hydrolysis of glycerophosphlipids and sphingomyelin in the outer membrane leaflet is brought about by treatment of intact red blood cells with phospholipase a2 and sphingomyelinase c, the (ca2+ + mg2+)-atpase activity is not affected. 2. complete hydrolysis of sphingomyelin, by treatment of leaky ghosts with spingomyelinase c, does not lead to an inactivation of the (ca2+ + mg2+)-atpase. 3. treatment of ghosts with phospholipase a2 (from either procine pancreas of naja naja veno ...	1977	137746
serotypes of bacillus cereus from outbreaks of food poisoning and from routine foods.	a provisional serotyping scheme was used to type cultures of bacillus cereus from 84 outbreaks of food poisoning in seven countries; 283 of the 337 (84%) cultures tested were typable. in 35 of the 61 outbreaks associated with a vomiting-type syndrome, foods, clinical specimens or both yielded h-serotype 1 only. type 1 strains together with other sterotypes were isolated in seven outbreaks. in 14 outbreaks types 3, 4, 5, 8 or a mixture of serotypes were present. untypable strains were isolated in ...	1977	401846
bacillus cereus pneumonia. survival in a patient with cavitary disease treated with gentamicin.	cavitary pneumonitis due to bacillus cereus occurred in a patient with acute lymphoblastic leukemia. pneumonia due to this organism is uncommon and recovery has not been previously reported. the present report is the first description of cavitary pneumonia due to b. cereus with a successful outcome. recovery was attributed to appropriate antimicrobial therapy and induction of leukemic remission with chemotherapy.	1977	402096
biochemical properties of clostridium bifermentans spores.	as previously found for spores of bacillus species, dormant spores of clostridium bifermentans contained essentially no adenosine triphosphate, a high level of adenosine monophosphate, a high level of 3-phosphoglyceric acid, and much transfer ribonucleic acid lacking a 3'-terminal adenosine monophosphate residue. as in spores of bacillus species, germination of c. bifermentans spores was accompanied by utilization of the 3-phosphoglyceric acid, a large increase in the adenosine triphosphate leve ...	1977	402349
rapid fluorimetric assay of minocycline in plasma or serum: comparison with microbiological assay.	1. plasma concentrations of minocycline were assayed by measuring the fluorescence emission of an aluminum-minocycline chelate in a spectrofluorimeter. 2. the assay gives reproducible results over the concentration range 0.05-5.0 mug ml-1, and compared favourably with results achieved using a conventional large plate agar diffusion microbiological assay in a double-blind comparison of the two methods. 3. fluorimetric assay of minocycline in plasma was found to correlate closely with antibiotic a ...	1977	402926
initiation of bacillus spore germination by hydrostatic pressure: effect of temperature.	suspensions of bacillus cereus t, b. subtilis, and b. pumilus spores in water or potassium phosphate buffer were germinated by hydrostatic pressures of between 325 and 975 atm. kinetics of germination at temperatures within the range of 25 to 44 degrees c were determined, and thermodynamic parameters were calculated. the optimum temperature for germination was dependent on pressure, species, suspending medium, and storage time after heat activation. germination rates increased significantly with ...	1977	403174
phospholipase c from bacillus cereus. evidence for essential lysine residues.	1. phospholipase c was inactivated by exposure to the three amino-group reagents, ethyl acetamidate, 2,4,6-trinitrobenzensulphonic acid and pyridoxal 5'-phosphate plus reduction. 2. inactivation by pyridoxal 5'-phosphate showed the characteristics of schiff's base formation with the enzyme. the pyridoxal 5'-phosphate-treated enzyme after reduction had an absorbance maximum at 325 mm and 6-n-pyridoxyl-lysine was the only fluorescent component after acid hydrolysis. 3. for complete inactivation, 2 ...	1977	403907
enzymic assays for isomers of 2,6-diaminopimelic acid in walls of bacillus cereus and bacillus megaterium.	an enzymic assay for individual isomers (meso-, ll- and dd-) of 2,6-diaminopimelate was developed. the enzyme 2,6-diaminopimelate decarboxylase specifically attacked meso-diaminopimelate and was used to measure this isomer manometrically. the meso- and ll-isomers were measured together manometrically in a coupled assay with diaminopimelate decarboxylase and diaminopimelate epimerase (which converts ll-diaminopimelate into meso-diaminopimelate). the dd-isomer was not attacked by either enzyme and ...	1977	403912
role of glutamate in the sporogenesis of bacillus cereus.	bacillus cereus t, sporulating in a chemically defined medium under optimum conditions, requires substrate quantities of glutamate during the first 4 h of sporogenesis. seventy percent of the glutamate utilized was catabolized to co2 during this period, with the remaining glutamate carbon assimilated into various spore constituents, principally protein and nucleic acid. the importance of glutamate as the primary source of reducing potential and energy for early stages of spore formation was inve ...	1977	404283
precipitating antibody against protease of corynebacterium pyogenes in pigs.	antibody in sera from pigs carrying an abscess associated with corynebacterium pyogenes and healthy pigs was examined by the agar gel diffusion test. in the test, the concentrated culture fluid containing the protease of c. pyogenes was used as antigen. as a result, precipitating antibody was demonstrated in sera from 25 of 30 abscessed pigs and a few of the healthy pigs. when the relationship between precipitating antibody and protease was examined by the immunoelectrophoresis and gel filtratio ...	1977	404574
inactivation of phospholipase c from bacillus cereus by a carboxyl group modifying reagent.	phospholipase c from bacillus cereus was inactivated by incubation with either of the carboxyl reagents, a water-soluble carbodimide plus a nucleophile or woodward's reagent k. with the former reagent, the incorporation into the enzyme of the first mol of nucleophile caused a 4-5-fold increase in the km for dihexanoyllecithin with no significant effect on the vm. the second mol of nucleophile incorporated caused no further change in km but destroyed most of the catalytic activity. modification o ...	1977	404815
the inactivation of beta-lactamase i from b. cereus by dicloxacillin: effect of buffer composition on the kinetic characteristics of the modified enzyme.	buffer composition affects the pattern of inactivation of b. cereus beta-lactamase i by dicloxacillin. at ph 7.0, in the presence of phosphate ions, dicloxacillin appears to cause essentially an irreversible inactivation of the catalytic site of the enzyme, while in a tris-cacodylate buffer the inactivation process appears to affect essentially the substrate-binding site. buffer ions act presumably by interacting themselves with either the catalytic or the substrate-binding site of the enzyme, t ...	1977	405348
microbial production of vitamin b12 antimetabolites. iii compound 102804 from bacillus cereus.	a basic compound with empirical formula c12h16n2o5 was isolated from bacillus cereus 102804 fermentations of a soybean meal-glucose medium. the inhibitory activity of compound 102804 on growth of gram-positive and gram-negative bacteria growing in a chemically defined medium was reversed by vitamin b12, by l-methionine, and by d-methionine. it has no inhibitory activity for escherichia coli (davis 113-3) when grown in media containing l-methionine. the biosynthesis of 102804 coincides with the s ...	1977	405359
an investigation of the bacteriological quality of retail vanilla slices.	one hundred and thiry-three vanilla slices, purchased from shops in the west yorkshire metropolitan county, were examined to determine the numbers and types of bacteria present at the time of purchase. the surface colony count at 37 degrees c was greater than 10(3)/g in 67/133 (50%) of the samples examined, bacillus cereus being found at that concentration in 21.8%, coliform bacilli including e. coli in 5.3%, staphylococcus aureus in 3-0% and streptococcus faecalis in 0-8%. thirty-four strains o ...	1977	405420
fate of foreign dna taken up by tonsillar lymphocytes.	the uptake of heterologous (b. cereus) dna by tonsillar lymphocytes was followed for 22 hours. acid-precipitable dna became associated with the cells as soon as they were mixed with dna. the uptake was linear from 0 to 3 hours, between 3 to 6 hours part of the cell-associated dna was released into the incubation medium, and at 22 hours the amount of label in the cells reached the values measured at 2--3 hours. in respect of the size of dna the uptake seemed to follow the same saturation curve. t ...	1977	405837
a severe necrotic enterotoxin produced by certain food, food poisoning and other clinical isolates of bacillus cereus.	the ability of certain strains of bacillus cereus consistently to elaborate a filterable non-dialysable toxin capable of causing severe disruption and necrosis of the intestinal mucosa and submucosa is confirmed. this property is not universal to all b. cereus strains and different degrees of production of this toxin are exhibited by the different strains which produce it. the necrotic effect is produced by whole-cell cultures of the toxin producing strains in broth and in boiled rice. some char ...	1977	405982
severe systemic and pyogenic infections with bacillus cereus.		1977	406000
the effect of storage on drugs incorporated into pelleted fish food.		1977	406257
a phosphatidylinositol phospholipase c (phosphatasemia factor)of bacillus cereus.		1977	406439
study of inhibition of outgrowth in bacillus cereus t by ethyl picolinate.	the effects of ethyl picolinate on germination, outgrowth, and sporulation of bacillus cereus t were studied in a synthetic medium containing glucose. ethyl picolinate specifically inhibited at two stages, outgrowth and sporulation. the initiation of germination and cell division was not affected. the inhibition of outgrowth by ethyl picolinate could be reversed by enrichment of inoculum with aspartic acid, asparagine, lysine, phenylalanine, and tyrosine among the amino acids and by oxalacetate. ...	1977	406840
microbiological transformations of delta6a10a-tetrahydrocannabinol.	a screening program was conducted to find microorganisms that catalyze transformation reactions with cannabinoids. three hundred fifty-eight cultures, consisting of 97 bacteria, 175 actinomycetes, and 86 molds, were incubated in media containing 0.5 mg of delta(6a,10a)-tetrahydrocannabinol (delta(6a,10a)-thc) per ml. after 120 h of cultivation, ethyl acetate extracts of the cultures were examined by thin-layer chromatography (tlc) for transformation products. about 18% of the cultures modified d ...	1977	406841