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transport of proteins into mitochondria: a high conservation of precursor uptake and processing system.ornithine transcarbamylase (ec 2.1.3.3) of rat (rattus norvegicus var. albus) liver, a urea cycle enzyme, is synthesized extramitochondrially as a larger precursor which is transported posttranslationally into mitochondria and processed to the mature enzyme. the precursor synthesized in vitro was taken up and processed to the mature enzyme by isolated pigeon (columba livia var. domestica) liver and frog (rana catesbeiana) liver mitochondria. carp (cyprinus carpio) liver mitochondria could also p ...19836347507
high mutagenic potency of several polycyclic aromatic hydrocarbons induced by liver postmitochondrial fractions from control and xenobiotic-treated immature carp.the metabolism of carcinogens in fish was examined by measuring the activation of different polycyclic aromatic hydrocarbons (pah) by carp (cyprinus carpio l.) liver post-mitochondrial fractions (s9) using the salmonella typhimurium ta100 reverse mutation assay. for this study, 1 non-carcinogen, anthracene (an), and 4 carcinogens, chrysene (chr), benzo[a]pyrene (bap), 3-methylcholanthrene (3mc) and 7,12-dimethylbenzanthracene (dmba), were chosen. the bioactivating potency of the metabolic system ...19836348528
purification and properties of enolase from carp (cyprinus carpio). comparison with enolases from mammals' muscles and yeast.the enolase (2-phospho-d-glycerate hydrolyase e.c. 4.2.1.11) from carp muscle was obtained, the specific activity--88 u/mg of protein. km for 2-phosphoglycerate was 0.313 mm and for phosphoenolpyruvate--0.76 mm. the enzyme is active only in the presence of divalent metal ions, mg2+ being the best activator. the phosphate and fluoride decreased the activity of enzyme. the molecular weight of the dimeric form of enzyme was found to be 93,000. the enzyme is immunologically different from pig muscle ...19836352164
[quantitative determination of spring viremia carp virus (svcv) antigens using immunofluorescence and immunoperoxidase plaque technic]. 19836353812
immunochemical analysis of cod fish allergen m: locations of the immunoglobulin binding sites as demonstrated by the native and synthetic peptides.the major allergen of codfish (allergen m) is a muscle protein belonging to the family of calcium binding parvalbumins. the primary structure of the molecule was established and the molecular weight was estimated from the sequence data to be 12,328. allergen m consists of 113 amino acid residues and one residue of glucose. a molecular arrangement of three domains (ab, cd and ef--the latter two bind one ca2+ ion each) was described for allergen m, analogous to carp parvalbumin pi 4.25. the sugges ...19836356964
effect of synthetic lys-trp on adenosine triphosphatase activity of carp and rabbit myosin bs.1. with carp myosin b atpase, the acceleration effect was found at peptide concentration ranging between 1 nm and 1 mm with a maximum at 10 nm (30% acceleration) at the low myosin b concentration (0.2 mg/ml). at the high myosin b concentration (0.8 mg/ml) a maximum was observed at 1-10 mm (10% acceleration). 2. with rabbit myosin b atpase, the acceleration (10%) was found only at a microm of lys-trp in the case of low myosin b concentration (0.2 mg/ml). under the high concentration of rabbit myo ...19836221861
morphological and biochemical studies on liver, kidney and gill of fishes affected by pesticides.effect of a herbicide, paraquat (1,1'-dimethyl-4,4'-bipyridilium-dichloride), the fungicide copper sulphate, and zinc chloride was studied on the histological structure of liver, kidney and gill of three fish species with different feeding habits, viz.: a herbivorous, silver carp (hypophthalmichthys molitrix); an omnivorous, common carp (cyprinus carpio l.) and a carnivorous, sheatfish (silurus glanis l.). the organs were studied electron microscopically after fixation according to karnovsky. th ...19836230560
ontogeny of the immune system in carp (cyprinus carpio l.). the appearance of antigenic determinants on lymphoid cells detected by mouse anti-carp thymocyte monoclonal antibodies.mouse anti-carp thymocyte monoclonal antibodies were used to investigate the appearance of lymphoid cell determinants in young carp. determinants detected by clone wct 4 were present on thymocytes as soon as the thymus was visible histologically, day 4 after fertilization (a.f.) at 21 degrees c, but wct 4-positive lymphocytes were not present in the pronephros until day 7 a.f.. in contrast, determinants detected by monoclonal antibodies from clones wct 8 and wct 10 appeared on thymocytes from da ...19836196239
sulfomucins and sialomucins in the mucus cells of the alimentary canal in an indian freshwater major carp, labeo rohita (hamilton): a fluorescence microscopic study.the localization of sulfomucins and sialomucins present in the various types of mucus cells--secretory (smc) and mature non-secretory mucus cells (mmc) of the different regions of the alimentary canal in labeo rohita (ham.) has been studied by fluorescence microscopical techniques (viz., ferric alum coriphosphine--fac, coriphosphine thiazol yellow--cty and dische reaction--dr). high degree of fluorescence activity confirms the presence of relatively high amount of sulfomucins in the mucus cells ...19836199266
a cell-free soluble-phase radioimmunoassay for thy-1 antigen.a cell-free, soluble-phase, radioimmunoassay has been developed for thy-1 antigen. the method is based on immunoprecipitation of radiolabelled thy-1 molecules with specific antibodies, anti-immunoglobulin serum and polyethyleneglycol (peg). the method can be used with convenience to screen for the presence of thy-1 in various fluids as well as on cell surfaces for qualitative or quantitative purposes. presence of antibodies or autoantibodies against thy-1 can also be detected specifically. evide ...19836140218
interaction of the component enantiomers of the putative dopamine autoreceptor agonist, tl-99 (6,7-dihydroxy-2-dimethylamino tetralin) with dopaminergic systems in mammalian brain and teleost retina.the enantiomers of the putative dopamine autoreceptor agonist, tl-99 (6,7-dihydroxy-2-dimethylaminotetralin) were examined in a number of in vivo and in vitro test paradigms to further examine the reported autoreceptor selectivity of this compound. the (+)-isomer of the aminotetralin was more active as a dopamine agonist than either the racemate or the (-)-enantiomer. in addition to this dopaminergic activity, tl-99 was found to be a potent alpha 2-adrenoceptor agonist, this activity being more ...19836141532
[infestation of larval trematodes from fresh-water fish and brackish-water fish in river taechong, kyungpook province, korea]a study of infestation patterns for larval trematodes from fresh-water fish and brackish-water fish was carried out during the period from june to october in 1982. they were collected by netting, fishing with rod and line, and using the bait of crushed oil cake in a transparent plastic bowls at the three localities, daebon-dong, kugil-dong, and hoam-dong in the river taechong, kyungpook province, korea. five species of fresh-water fish, the crussian carp, carassius carassius linnaeus, the bullhe ...198312902663
[experimental infection of clonorchis sinensis to cyprinus carpio nudus]as a part of observation on the suitability as a second intermediate host of clonorchis sinensis, various infection experiments were tried to cyprinus carpio nudus. the results obtained were summarized as follows: 1. the cercariae which attempted to contact with cyprinus carpio nudus in the water were observed under the stereomicroscope. after contact, cercariae began to separate its tails from the bodies at 4 minutes increasingly, and then the number ranged to 80 percent at 13 minutes after the ...198312902664
[the wormicidal substance of fresh water fishes on clonorchis sinensis: iv. preliminary research on the wormicidal substance from mucus of cyprinus carpio nudus]as a series of studies on the suitability as a second intermediate host of clonorchis sinensis, the wormicidal effects of mucus from epidermis of cyprinus carpio nudus were observed. the results obtained were summarized as follows: on the silica gel thin layer chromatography with the greenish yellow supernatant in acetone:benzene/10 : 90 as carrier, seven spots were observed in iodine chamber. the spot of rf. 0.225 value among them disclosed the strongest wormicidal effect on the cercaria, the e ...198312902665
chlorinated paraffins: disposition of a polychloro-[1-14c]-hexadecane in carp (cyprinus carpio) and bleak (alburnus alburnus).the disposition of polychloro-[1-14c]hexadecane (pchd, 34% chlorine w/w) was studied in carp (cyprinus carpio) and bleak (alburnus alburnus). after intra-arterial (i.a.) injection in carp about 6% of dose was excreted as 14co2 in 96 h. the autoradiographic distribution pattern after administration of pchd to carp (i.a.) and bleak (up to 14 days of exposure in pchd-contaminated water) was characterized by a strong labelling in the bile/intestinal contents. high radioactivity also appeared in the ...19836419402
the biotransformation of three 14c-labelled phenolic compounds in twelve species of freshwater fish.1. the urinary and biliary excretion of 14c-labelled m-cresol, 1-naphthol and o-chlorophenol were investigated in 12 species of freshwater fish (bitterling, rhodeus sericeus amarus; bream, abramis brama; crucian carp, carassius carassius; goldfish, carassius auratus; gudgeon, gobio gobio; guppy, poecilia reticulata; minnow, phoximus phoximus; perch, perca fluviatilis; roach, rutilus rutilus; rudd, scardinius erythropthalmus; three-spined stickleback, gasterosteus aculeatus; tench, tinca tinca) w ...19836880238
development of a heterologous radioimmunoassay for eel (anguilla anguilla) gonadotropin.a heterologous radioimmunoassay (ria) for eel gonadotropin (gth) was developed, using an antiserum to the beta subunit of carp gth (cgthbeta). partially purified eel gth preparations or crude eel pituitary extracts produced partial cross-reactions: the inhibition curves reached a plateau before complete inhibition of the binding of the tracer and the slopes were lower than those for cgth. however, the parallelism between the inhibition curves produced by all the eel samples allowed estimation of ...19836301935
connections between photoreceptors and horseradish peroxidase-injected bipolar cells in the carp retina.we describe two physiologically and morphologically distinct types of on-center bipolar cell in the carp retina. these cells can be distinguished from one another in the following respects: (1) "type i" cells respond to moderate light intensities with a transient depolarization followed by a plateau, while the response of "type ii" cells is approximately rectangular in shape; (2) latency of type ii cell responses is shorter than that of type i cell responses; (3) mean diameter of type ii cell de ...19836880034
cytoarchitecture and topographic projections of the gustatory centers in a teleost, carassius carassius.the neuronal connections in the central gustatory system of the crucian carp were examined by means of degeneration and hrp methods. cell morphology in the primary gustatory lobes was studied in golgi-impregnated material. medium-sized neurons of the facial lobe emit axons which project to the secondary gustatory nucleus. the nucleus intermedius facialis of herrick ('05) projects bilaterally. large neurons send axons through the spinal trigeminal tract to terminate in the spinal trigeminal nucle ...19836619321
morphologic changes in teleost primary and secondary retinal cells following brief exposure to light.light adaptive morphologic changes in the teleost retina, such as movements of rods, cones, and epithelial pigment and spinule formation on horizontal cell terminals, are normally associated with continual exposure to light. depending on a variety of factors these processes are generally completed within 30-60 min. in this report we show that a brief exposure to light (1-2 min) preceded and followed by darkness is sufficient to elicit these changes in four species of teleost; the trout (salmo ga ...19836826311
calcium binding proteins: optical stopped-flow and proton nuclear magnetic resonance studies of the binding of the lanthanide series of metal ions to parvalbumin.optical stopped-flow techniques have been used to determine the dissociation rate constants (koff) for the lanthanide(iii) ions from carp (pi 4.25) parvalbumin. for most of the 13 different lanthanides studied, the release kinetics were diphasic, composed of both a fast phase (whose rate varied across the series, la3+ leads to lu3+, between the limits -1.2 less than or equal to log kfast less than or equal to -0.7) and a slower phase (whose rate varied across the series, la3+ leads to lu3+, betw ...19836661415
[value of ms associated factors for test procedures in multiple sclerosis: 1. carp's ms associated agent in the serum].the determination of multiple sclerosis-associated factors in serum is grounded on the conception that there exist virus-like particles with a special predicative value for diagnosis. a test system, elaborated by carp, is basing on the reduction of neutrophilic granulocytes in peripheral blood of mice by a ms-associated agent (msaa) of serum, cerebrospinal fluid, brain and other organs of ms-patients. several groups and the present investigators did not always receive constantly positive finding ...19836669653
a simple method for collecting 14co2 in fish.a simple apparatus was designed for continuous collection of excreted 14co2 in fish. carp (cyprinus carpio; body wt. about 400 g) were intraarterially injected with [14c]acetate (44 mmol) and transferred to a test chamber (21 +/- 1 degrees c). within 48 h, 65.4 +/- 2.7% of the dose was recovered as 14co2. a small amount of 14co2 was retained in the water of the test chamber. control experiments using [14c]acetate-injected mice as '14co2-generators' showed that this 14c residue in the water could ...19836419401
mechanism of the water response in carp gustatory receptors: independent generation of the water response from the salt response.the carp gustatory responses to various salts and those to distilled water after adaptation of the receptors to the salts were recorded from the palatine nerve under varying conditions. (a) as far as na4fe(cn)6 solution prepared freshly was used, any peak in the dose-response curve was not observed, while the solution stored overnight induced a large peak response at the low concentration region as konishi reported. the magnitude of the water response after adaptation to the stored solution was ...19836640337
effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes.spermatozoa of freshwater cyprinidae (goldfish, carp, crucian carp and dace) remained immotile when the semen was diluted in solutions of nacl, kcl, mannitol or glucose iso-osmolar to the seminal plasma (300 mosmol kg-1). the spermatozoa became motile in media containing these solutes if the osmolality was lower than that of the seminal plasma, suggesting that motility is suppressed by the osmolality of the seminal plasma in the sperm duct and initiated by a decrease of osmolality upon spawning ...19836421979
effect of acclimation temperature on intraerythrocytic acid-base balance and nucleoside triphosphates in the carp, cyprinus carpio.in carp acclimated to 20 degrees c or 10 degrees c intraerythrocytic ph (phi) and plasma ph (phe) were determined in vitro after equilibration with co2 in either o2 or n2. atp and gtp were determined with an enzymatic assay described in detail. the relationship between phi, phe and oxygen saturation was not affected by the acclimation temperature and was (phi-6.10) = (0.853-0.159 x s) x (phe-6.21) there was a slight but significant decrease in atp at 20 degrees c. apparent buffer values were aff ...19836420857
effects of vasoactive intestinal peptide and other peptides on cyclic amp accumulation in intact pieces and isolated horizontal cells of the teleost retina.the effects of vasoactive intestinal peptide (vip) and several other peptides have been examined on cyclic amp accumulation in intact pieces and isolated horizontal cells of the teleost (carp) retina. vip was the most effective peptide examined, inducing a dose-related response, and an approximately fivefold increase in cyclic amp production when used at a concentration of 10 microm. porcine histidine isoleucine-containing peptide and secretin, peptides structurally related to vip, also stimulat ...19836194261
treatment of ornamental koi carp (cyprinus carpio) infected with anchor worms (lernaea cyprinacea). 19836649375
characterization of the 42-s nucleoprotein particles of cyprinus carpio oocytes.previtellogenic oocytes of the fish cyprinus carpio contain 42s nucleoprotein particles that are composed of two proteins of molecular weights 48,500 and 39,300 (molar ratio 2:1), trna and 5s rna (molar ratio 3:1). the trna population embodied in the 42s particle contains all amino acid acceptor species but their distribution differs from that found in trna from mature oocytes.19836679738
inhibition of carp liver mitochondrial monoamine oxidase by some commonly-used detergents.the inhibitory effects of some detergents commonly used in biochemical research on carp liver mitochondrial monoamine oxidase were examined. sodium dodecylsulfate, octyl-beta-d-glucopyranoside, sodium cholate and triton x-100 at relatively low concentrations caused strong dose-dependent inhibition of the activity towards tyramine, but digitonin caused only weak inhibition. sodium dodecylsulfate, octyl-beta-d-glucopyranoside and sodium cholate caused almost complete inhibition of activity in the ...19836645111
molecular mechanisms of odor sensing. vi. some biochemical characteristics of a possible receptor for amino acids from the olfactory epithelium of the skate dasyatis pastinaca and carp cyprinus carpio.the 25 000 x g pellet of skate and carp olfactory tissue was found to contain structures with high affinity for amino acids (kd approximately 10(-10) m). results from competition experiments suggest that a multiplicity of types of olfactory binding sites exists in the skate and carp. some parameters of a possible receptor for amino acids were evaluated. the isoelectric point of these sites solubilized with triton x-100 was 5.35 and the molecular weight was about 120 000-150 000.19836882801
use of lanthanide-induced nuclear magnetic resonance shifts for determination of protein structure in solution: ef calcium binding site of carp parvalbumin.the binding of the paramagnetic lanthanide ion ytterbium to the calcium binding protein carp parvalbumin results in a series of 1h nmr resonances which are shifted far outside the envelope of the 1h nmr spectrum of the diamagnetic form of the protein; bound yb3+ also induces shifts in the 13c nmr spectrum of parvalbumin and in the 113cd nmr spectrum of cadmium-substituted parvalbumin. the interpretation of these lanthanide-shifted resonances in terms of the structure of the protein surrounding t ...19836626506
role of cations in olfactory reception.the effects of changed ionic environments on the olfactory responses in the carp, the rainbow trout, and the bullfrog were examined by recording the stimulant-induced waves from the olfactory bulb. (a) application of stimulants (various species of amino acids and beta-ionone) dissolved in deionized water to the edta-treated olfactory epithelium of the carp did not induce any response. the addition of various species of salts to the stimulating solution reversibly restored the response. (b) the r ...19836605175
vasoactive intestinal peptide alters membrane potential and cyclic nucleotide levels in retinal horizontal cells.vasoactive intestinal peptide stimulated the synthesis of adenosine 3',5'-monophosphate in fractions of isolated carp horizontal cells. when applied extracellularly to isolated and cultured horizontal cells, the peptide also induced a slow depolarization (30 to 40 millivolts) accompanied by a decrease in membrane resistance. however, analogs of adenosine 3',5'-monophosphate applied extracellularly or intracellularly, and forscolin applied extracellularly, had no effect on the membrane potential ...19836308770
the dynamics of the steroidogenic response of perfused xenopus testis explants to gonadotropins.the dynamics of androgen production by perfused xenopus testis explants has been investigated under various gonadotropic stimulations. androgens were measured in perfusate by radioimmunoassay. the behavior of hormones in the perfusion system was assessed. pieces of testis were capable of producing androgens after more than 20 hr in perifusion. amplitude and duration of the response to a gonadotropic stimulation were the same 3 and 10 hr after the beginning of the experiment. after stimulation by ...19836628970
high-pressure liquid chromatographic separation of a mixture of corticosteroids, androgens, and progestins.fifteen steroids including corticosteroids, androgens, progestins, and their derivatives were completely separated by reverse-phase high-pressure liquid chromatography on a chemcopak 7 ods-h column in 50 min. the elution procedures were first with water:methanol:acetonitrile:isopropanol 55:32:6.5:7.5 (v/v) for 15 min and followed with a linear gradient elution for 35 min from 0 to 80% of water:methanol:n-butanol 40:40:20 (v/v). the applicability of this method was successfully demonstrated in th ...19836625598
[gill structure in healthy carp].when the gills of carp are studied, this organ should be evaluated from there planes of projection: sagittal, transversal and horizontal. lamellae, the functional base of the gills, provide the exchange of oxygen between blood and water. lamellae consist of a network of sinuses with a skeleton formed by columnar cells and with walls constituted by the cytoplasmic processes of these cells. on the outer side the sinuses are covered with one or two layers of flat epithelial cells. the gill filament ...19836417877
an optical stopped-flow and 1h and 113cd nuclear magnetic resonance study of the kinetics and stoichiometry of the interaction of the lanthanide yb3+ with carp parvalbumin.the rate constants for the dissociation of the lanthanide yb3+ from the cd and ef calcium-binding sites of carp parvalbumin (isoelectric point, 4.25) have been measured using optical stopped-flow and 1h nuclear magnetic resonance (nmr) methods. the off-rate constants for yb3+ are 1.5 x 10(-1) and 1.3 x 10(-3) s-1, respectively, at ph 6.6 and 23 degrees c. the relative displacement of ca2+ from the two sites by yb3+ was determined from the observed amplitude of the fast and slow kinetic phases. y ...19836627097
the effect of temperature on physiological changes in carp, cyprinus carpio l., induced by phenol.carp were exposed to four sublethal phenol concentrations (0.01, 0.1, 1.0, 10 mg/liter) at two temperatures (12 and 17 degrees c). blood and tissue samples were taken after various periods (1-72 hr) of exposure. for each period controls were taken. serum concentrations of glucose, cortisol, protein, cholesterol, and triglycerides were determined. parallelly, the liver glycogen content and the relation of wet and dry weight of the white epaxial muscle were measured. no general significant differe ...19836617564
an intracellular study of the optic tectum of the carp in vitro.intracellular recordings were performed on the optic tectum of the carp in vitro. thick brain sections were prepared and the optic tract was electrically stimulated. epsps of 72 cells in stratum fibrosum et griseum superficiale, stratum griseum centrale and stratum album centrale were examine. monosynaptic epsps were recorded from 19 cells in stratum fibrosum et griseum centrale and stratum griseum centrale, which was consistent with anatomical findings.19836310457
new aspects on the 'rodlet cell' in teleosts.the enigmatic rodlet cells, hitherto interpreted mainly as unidentified parasitic stages ('rhabdospora thélohani') or as a sort of secretory cell, have been studied with electron microscopical and histochemical methods in three teleost species (cyprinus carpio l., tinca tinca l., salmo gairdneri rich.). the typical inclusions, the rodlets, consist of a dense core and a less dense cortex, with a coarse flocculent outer zone. the latter shows a positive reaction for neutral polysaccharides respect ...19836876223
a gaba antagonist, bicuculline, exerts its uncoupling action on external horizontal cells through dopamine cells in carp retina.external horizontal cells in isolated retinas of the carp (cyprinus carpio) were intracellularly recorded and marked with the fluorescent dye lucifer yellow (ly). these cells are electrically coupled via gap junctions, so that the injected dye normally diffused to neighboring cells. a gaba antagonist, bicuculline (bcc, 20 microm), applied to the vitreous fluid beneath the isolated retina, altered the spatial properties of light-induced responses, by increasing the amplitude of responses to centr ...19836310453
sunburn lesions in koi carp. 19836879972
ultrastructural immunocytochemistry of growth hormone cells in the goldfish pituitary gland.the immunocytological activity of an antiserum to carp growth hormone was studied on the goldfish pituitary gland, using a peroxidase-antiperoxidase ultrastructural technique. the reaction was specific for secretory granules in cells in the proximal pars distalis categorized as growth hormone cells on the basis of ultrastructural characteristics. the results support the specificity of the antiserum for growth hormone and provide firm identification of the growth hormone cells in goldfish.19836350103
development and validation of a carp growth hormone radioimmunoassay.purified growth hormone (gh), isolated from pituitary glands of the common carp (cyprinus carpio), was shown to have biochemical and immunological properties in common with other teleost gh preparations. intraperitoneal injections of the carp gh, at a dose of 1 microgram/g body wt, resulted in significant increases in body weight in goldfish. in addition, the carp gh was used to prepare an antiserum for the development of a radioimmunoassay (ria). the displacement curves for serum from goldfish ...19836193032
effect of acclimation temperature on oxygen transport in the blood of the carp, cyprinus carpio.the temperature dependence of the o2 equilibrium in whole blood was measured in carp acclimated for more than 3 weeks at 10 degrees c or 20 degrees c water temperature. o2 combining curves were obtained for the same samples at 10 degrees c or 20 degrees c using blood from the 10 degrees c or 20 degrees c acclimated fish (group a) or only at the acclimation temperature (group b). whereas in group b the p50 was about the same at both temperatures, in group a p50 was higher at 20 degrees c than at ...19836878907
immunocytochemical identification of the prolactin-secreting cells in the teleost pituitary with an antiserum to chum salmon prolactin.an antiserum raised to highly purified chum salmon (oncorhynchus keta) prolactin (sprl) was used to identify prolactin-producing cells in the adenohypophysis of 15 species of teleosts by the immunocytochemical peroxidase-antiperoxidase method. in the chum salmon, the only pituitary cells that reacted with sprl antibody were the prl cells organized as follicular structures in the rostral pars distalis. when the antiserum was absorbed with sprl, on the other hand, no immunoreactive cell was observ ...19836862175
the role of the retinal interplexiform cell: effects of 6-hydroxydopamine on the spatial properties of carp horizontal cells. 19836405977
induction of melanization in primary cultures of dermal cells from xanthic goldfish scale by adrenocorticotropic hormone and goldfish or red carp serum. 19836301695
carp as a source of eicosapentaenoic acid. 19836132079
[electrophysiological study of olfactory sensitivity in the carp].responses of single olfactory tract fibers cut off from prosencephalon to adequate stimulation of the carp olfactory epithelium revealed that the threshold sensitivity to aminoacids fell in the range between 10(-12) and 10(-10) m, that the magnitude and the form of the response depended on the concentration and type of stimulus as well as on the level of background activity. the results are compared with the biochemical data obtained earlier.19836406277
[growth and feed consumption of carp (cyprinus carpio l.) with different protein and energy supply. 2. weight gain, feed conversion, protein and energy consumption]. 19836845894
[growth and feed consumption of carp (cyprinus carpio l.) with different protein and energy supply. 1. experimental design, ration composition, digestibility]. 19836845893
respiratory responses to stimulation of branchial vagus nerve ganglia of a teleost fish.the effects of electrical stimulation of epibranchial vagus ganglia upon respiration of the carp were investigated. single shocks evoked fast twitch responses in a number of respiratory muscles with latencies around 18 msec to the beginning and 30-35 msec to the peak of activity. shocks given during abduction decreased the respiratory cycle duration by shortening abduction and accelerating adduction. stimuli given throughout most of adduction also shortened the respiratory cycle, accelerating th ...19836844758
[potentiation by pimozide of the effects of lhrh-a on hypophyseal gonadotropin secretion, ovulation and spermiation in the common carp (cyprinus carpio)].during the reproductive season in august, we administered to adult male and female carp an lhrh analog (lhrh-a = d-trp6ea-lhrh) (50 micrograms/kg) alone or in association with pimozide, a dopamine inhibitor (10 mg/kg). lhrh-a caused the circulating portal level of gonadotropin to rise significantly in males and less in females. spermiation was stimulated in the males and oocyte maturation in females but there was no ovulation. an infection of pimozide and then of lhrh-a 6 h later significantly i ...19836405982
management, nutrition and housing of farmed fish.to obtain optimum production in fish farming the oxygen level, ph and temperature of the water are important as well as proper feeding. various systems ranging from extensive to intensive are used for holding fish. in the uk salmonid fish are farmed most commonly. the different methods used for farming carp, turbot, eels and shellfish as well as trout and salmon are described.19836829155
dopamine modulates s-potential amplitude and dye-coupling between external horizontal cells in carp retina.horizontal cells in the fish retina are electrically coupled and possess gap junctions so that intracellularly injected dye normally diffuses freely to neighbouring cells. applied dopamine (da) alters the spatial properties of the horizontal cell responses to light, increasing the amplitude of photopic l-type s-potentials but decreasing their lateral spread. these effects have been attributed to the action of da on horizontal cell membrane resistance, particularly at the gap junctions, and our p ...19836401844
[free and peptide amino acid composition in the seminal fluid of the carp (cyprinus carpio)].free and peptidic amino acids were measured in the seminal plasma of carp. some acids such as glutamic acid and glutamine, taurine, glycine and proline were exceptionally abundant, while others like lysine, arginine and histidine were found in small amounts. generally, the amino acid level was higher in carp seminal plasma than in that of other vertebrate species. as this level was systematically higher than in the blood plasma, it is supposed that there was neoformation of an original fluid in ...19836404512
discrete nonlinear reduction model for horizontal cell response in the carp retina.in this paper we present a new model for the spatial behavior of the external horizontal cells in the carp retina which elucidates more precisely the mechanisms of their intercellular connections. the area and intensity effects of a brief light stimulus on the l-type response were recorded and analysed. we discovered clear nonlinearities in the behavior of the response which could not be explained by the simple summative model presently accepted. proposed here is a new model, the spatial reducti ...19836880039
synaptic organization of the indoleamine-accumulating neurons in the cyprinid retina.the distribution and synaptic connections of the indoleamine-accumulating neurons in the retinae of the goldfish and carp were studied by means of fluorescence and electron microscopy. the indoleamine-accumulating neurons were visualized after intravitreal injection and uptake of the indoleamine 5,6-dihydroxytryptamine. this labeling procedure produced a characteristic yellow fluorescence of the indoleamine-accumulating neurons and also characteristic ultrastructural changes in these cells. to a ...19836850750
postprandial metabolic changes in larval and juvenile carp (cyprinus carpio).ammonia-nitrogen excretion and oxygen consumption rates after a meal were followed in carp larvae and juveniles of different sizes, starting from early exogenous feeding and until they had reached about 1 g of body weight. there was an immediate rise in the nitrogen excretion rate after feeding; the amplitude and duration of this increase were affected by body weight (bw) and the amount of nitrogen consumed (ni) and could be described by the equation: 37.61 bw-0.311 ni0.802. endogenous nitrogen ...19836844717
regional difference in density of monoamine-accumulating cells of carp and catfish retinas.by means of a histofluorescence technique, a comparative study was conducted on the regional density of dopaminergic (da) and indoleamine-accumulating (ia) cells in carp (cyprinus carpio) and catfish (ictalurus punctatus) retinas. in order to enhance detection of fluorescent cells, noradrenaline (na; 5.0 micrograms) or a mixture of na (2.5 micrograms) and 5,6-dihydroxytryptamine (5,6-dht; 2.5 micrograms) was intravitreally injected into the eyes 2-3 hr before enucleation. da and ia cells were co ...19836842627
tegmental neurons controlling medullary respiratory centre activity in the carp.the dorsal mesencephalic tegmentum of the carp was explored for respiratory rhythmic neurons. the properties of two types of neurons which could be distinguished on the grounds of their firing pattern and their behaviour during paralysis and electrical stimulation were analysed. one group is involved in the control of a special type of respiration called bout-respiration and besides influences the frequency of the respiratory rhythm. the other group can modulate the output of the motor neurons o ...19836836201
ethambutol changes the color coding of carp retinal ganglion cells reversibly.the influence of ethambutol on retinal function was studied by recording ganglion cell responses in isolated carp retinas superfused with a ringer solution containing different concentrations of ethambutol (0 mg/liter, 10 mg/liter, 20 mg/liter, 30 mg/liter). the results indicate that ethambutol reversibly affects color opponency, without changing the sensitivity of the underlying receptor processes. the amacrine and bipolar cells are the most likely candidates to be affected by ethambutol.19836826308
the optic tectum of the carp: pyramidal neurons of the sfgs.we have studied the stratum fibrosum et griseum superficiale (sfgs) of the carp optic tectum with optic and electron microscopy. this stratum is a dense neuropil with disordered appearance, in which numerous neuronal bodies of different characteristics and variable distribution according to the tectal regions are intercalated, more abundant in the dorsomedial zones of the tectum. within these neuronal types, the most characteristic of sfgs are the large pyramidals of vertical development. such n ...19836672093
light-evoked and antidromic activation of ganglion cells of the carp retina in a chloride free medium. 19836666081
[phagocytosis and exocytosis of latex particles by retinal pigment epithelium of carp]. 19836660159
gaba and glycine effects on the bipolar cells of the carp retina.effects of gaba and glycine applied electrophoretically were examined on bipolar cells in the carp retina. when applied at the outer plexiform layer, gaba produced a small depolarization in on-center cells, but a small hyperpolarization in off-center cells. these effects were concluded to be mediated by photoreceptor cells. when applied at the inner plexiform layer, gaba produced a hyperpolarization in on-center cells but no response in off-center cells. this response was resistant to co2+ ions. ...19836659375
information processing in the system from light to horizontal cell response of the carp retina.in this paper, two types of experiments in the carp retina are presented. one is the analysis of responses to white-noise-modulated light at different average mean intensity levels recorded from l-type horizontal cells. for weak light, the responses fluctuated symmetrically around mean values. for brighter light, however, the distribution of amplitude of the response departed from the normal distribution. the deviation from the normal distribution was characterized in terms of symmetry rather th ...19836659373
cell water content in carp kidney tissue slices as influenced by various osmotic agents.determination of water content in the surviving kidney tissue of the carp (cyprinus carpio) is a convenient method for studying epithelial cell volume regulation under the influence of various osmotic agents and inhibitors. artificial media of abnormal tonicities were used both as a purely experimental tool and as an attempt to model the behaviour of the tissue under extreme (physiological or pathological) conditions. in a medium hypertonic with mannitol the inhibition of the sodium-potassium pu ...19836622558
spermiogenesis in the rainbow trout (salmo gairdneri). an ultrastructural study.in an ultrastructural study on the spermiogenesis of the rainbow trout (salmo gairdneri r.) four spermatogenetic stages were identified. in young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). subsequently, it became more homogeneous and started to condense in the form of coarse granules and fibers and then into fibrils associated in ribbon-like elements which eventually partly fused together. during early spermiogenesis, a juxtanuclear vacuole ...19836616570
[structure and function of the pigment epithelial cup of the carp eye]. 19836613764
separation of cathepsin d-like proteinase and acid thiol proteinase of squid mantle muscle.when the proteinases of the squid mantle muscle were extracted in the presence of dithiothreitol (dtt), the acid proteinase activity increased, indicating that the squid mantle muscle contains a considerable amount of the acid thiol proteinase. the crude extract hydrolyzed neither alpha-n-benzoyl-d,l-arginine-p-nitroanilide (bapa) nor azocasein, thus refuting the presence of cathepsins b and l in the mantle muscle. the cathepsin d-like proteinase and the acid thiol proteinase were separated by s ...19836349920
effect of 17 alpha-methyltestosterone, estradiol-17 beta and synthetic lhrh on production of gonadotropic hormone in pituitaries of rainbow trout (organ culture).pituitary glands from 6-month-old sexually immature female rainbow trout, salmo gairdneri, were kept in organ culture for 48 or 72 h. certain groups of pituitaries were cultivated for 48 h on either control medium or medium with 17 alpha-methyltestosterone (mt), or with estradiol-17 beta (e2) in concentrations of 8.5 x 10(-7) m. other groups of pituitaries were cultivated for 72 h on control medium, or for 48 h on either control medium or mt-medium or e2-medium, and subsequently for 24 h on medi ...19836349817
primary culture of normal pituitary cells of carp (cyprinus carpio) for the study of gonadotropin release.a method for preparing enzymatically dispersed pituitary cell cultures of carp (cyprinus carpio) is described. the cultures have been used to assay a synthetic analog of gonadotropin releasing hormone (gnrh) and to determine the specificity of steroids able to affect gonadotropin (gth) release in vitro. time course secretion studies indicated that by 48 h incubation, in the presence of 500 pm gnrh, cumulative secretion of gonadotropin (719 ng +/- 90/2.5 x 10(5) cells) had exceeded that of contro ...19836337086
studies on the biology and pathology of the common carp parasite myxobolus basilamellaris lom et molnár, 1983 (myxozoa: myxosporea). 19836234773
muscle fibre composition of the eye muscles of the carp (cyprinus carpio l.) defined on the base of histochemical observations.a histochemical study has been carried out on the eye muscles of the carp. on the base of the atp-ase and sdh activity and with regard to the localization and diameter of muscle fibres six types of muscle fibres situated in the defined zones can be distinguished. the type 1 and 2 fibres display a moderately high atp-ase activity at ph 9.4 and rather low activity after alkaline and acid preincubation. type 1 shows a high sdh activity in contrast to type 2 with a low sdh activity. the other four t ...19836224725
pharmacology of the outer retina.the pharmacology of the outer retina is briefly reviewed, and some recent experiments on the pharmacology of isolated horizontal cells from the carp retina are described. our results indicate that there are at least three classes of neurotransmitter receptors on carp horizontal cell. one receptor type interacts with l-glutamate, a second with dopamine, and a third with vasoactive intestinal peptide.19836147035
seasonal variations in the activities of peroxide metabolism enzymes in erythrocytes of freshwater fish species.superoxide dismutase, catalase and peroxidase activities were estimated in erythrocytes of the carp, the tench and the crucian carp. the enzymes showed higher activities in spring (1982) than in autumn (1981). in both seasons the highest levels of these antioxidative defense enzymes were found in erythrocytes of the crucian carp.19836138208
pharmacological properties of isolated fish horizontal cells.at least three distinct receptors for neurotransmitter substances are present on carp horizontal cells. activation of two of the receptor types, by dopamine and vasoactive intestinal peptide respectively, results in the accumulation of cyclic amp within the cells. activation of the third receptor type, by l-glutamate or its analogues, causes a large, 60-80 mv depolarization of the cells. similar glutamate receptors are found on skate horizontal cells, which also possess receptors specific for ga ...19836136124
studies on the effect of paraquat on glycogen mobilization in liver of common carp (cyprinus carpio l.).1. a herbicide, paraquat (1,1'dimethyl-4,4'-bipyridilium-dichloride) was administered to carp in 0.5-10.0 ppm concentrations, respectively, and blood sugar level, glucose-6-phosphatase and glycogen phosphorylase activities of liver were determined. 2. paraquat treatment caused an increase of blood sugar level and enhanced phosphorylase and glucose-6-phosphatase activities. 3. paraquat can induce alterations in endoplasmic reticulum that might contribute to the changes in glucose-6-phosphatase ac ...19836135554
comparative aspects of protein digestion and amino acid absorption in fish and other animals.1. molar concentrations of amino acids in the content of subsequent parts of alimentary tract of rainbow trout and common carp have been calculated. 2. the ratio of free amino acid concentration in intestinal content to blood of carp most commonly was between 10 and 20 and decreased as digestion proceeded. 3. free amino acid concentration in gut content of fish appeared several times higher than that in reptiles or mammals. 4. absorption rate of amino acids in carp was most intense during the fi ...19836131785
effects of intraocular injections of 6-hydroxydopamine on dopamine-dependent cyclic amp accumulation in intact pieces of carp retina.dopamine-stimulated cyclic amp accumulation was measured in intact pieces of carp retina following intraocular injections of the dopaminergic neurotoxin 6-hydroxydopamine (6-ohda). this treatment is known to induce a selective destruction of dopaminergic nerve terminals, and in these experiments caused a 94% reduction in retinal dopamine content. however, dopamine-dependent cyclic amp accumulation was essentially unaltered in retinas exposed to 6-ohda, indicating that the dopamine receptors link ...19826295556
the mode of action of carp gonadotropin on the stimulation of androgen production by carp testis in vitro. 19826292039
inhibition of postsynaptic dopamine-sensitive adenylate cyclase in carp retina by the putative dopamine autoreceptor agonist, 3-ppp. 19826290231
neuronal addition and retinal expansion during growth of the crucian carp eye.using standard paraffin technique the addition of new cells in crucian carp retinas was examined. between eye diameters 4.4 and 10.0 mm the number of ganglion cells increases from 103,000 to 205,000, inl cells from 1.5 to 3 million, comes from 250,000 to 900,000, and rods from 2 to 9 million. concomitantly retinal area increases fivefold and the cell densities decrease by 37% for the cones, 57% for the inl cells, and 58% for the ganglion cells, while the rod density remains stable. in relation t ...19827130456
5-hydroxytryptamine stimulates [3h]dopamine release from the fish retina.serotonin (5-hydroxytryptamine, 5-ht; 0.5 micro m and above) stimulated the release of [3h]dopamine ([3h]da) from particulate fractions of the carp (cyprinus carpio) retina. the 5-ht effect was dose- and ca2+-dependent, and was structurally specific. a similar response was not elicited by the other indoles (5,6-dihydroxytryptamine, 5,7-dihydroxytryptamine, 5-hydroxytryptophan, or 5-hydroxyindoleacetic acid) examined. an increase in [3h]da release was elicited by addition of 5-ht agonists (5-meth ...19826953163
temperature dependence of the deformability of carp (cyprinus carpio) red blood cells. 19827106253
changes of genetic parameters in successive gynogenetic generations and some calculations for carp gynogenesis.in artificial gynogenesis diploidization of the sperm activated zygote is achieved by retention of the second polar body and by this method perfectly normal and fully viable broods can easily be reared. studying this form of propagation, we determined the coefficient of inbreeding (f), the fixation index (p) and the degree of genotypic identity (i) introduced for characterizing the isogeneity of gynogenetic populations with the help of the parameters of the probability distribution of meiotic re ...198224270755
oxygenation and deoxygenation kinetics of red cells in isolated lamellae of fish gills.1. the kinetics of oxygen transfer across the water-blood barrier in fish gills were studied by measuring oxygenation and deoxygenation of single red blood cells contained within isolated secondary lamellae using a microphotometric technique. 2. recordings of the overall time courses show that both oxygenation and deoxygenation are more rapid in the carp than in the eel. in both species the half-time for deoxygenation is about five times slower than for oxygenation. 3. it is shown that the resis ...19827108432
pcbs and other xenobiotics in raw and cooked carp. 19826809082
acetylcholine and substance p: action via distinct receptors on carp retinal ganglion cells.substance p (sp), a neuropeptide, has been found in amacrine cells in a variety of vertebrate retinas. sp excited many of the ganglion cells we sampled in carp retina; many of these ganglion cells are directly excited by cholinergic agonists. it has been reported that sp modulates cholinergic synaptic transmission in some other neuronal systems by inhibiting or desensitizing cholinergic receptors. sp does not act in this way on the ganglion cell cholinergic receptors in isolated carp retina. pul ...19826176567
demonstration of inositol hexaphosphate induced changes in structure at ligand binding sites in carp hemoglobin carbonyl. 19827103982
[morphodifferentiation of the pharyngeal teeth of the carp, cyprinus carpio linne]. 19826957065
predictability of pcbs in carp harvested in saginaw bay, lake huron. 19826807380
fish cell culture: initiation of a line of pituitary cells from carp (cyprinus carpio) to study the release of gonadotropin in vitro. 19826749652
calcium alters the sensitivity of intact horizontal cells to dopamine antagonists.horizontal cells of the carp retina possess dopamine receptors linked to adenylate cyclase. isolated, intact horizontal cells respond to micromolar concentrations of dopamine, whereas nanomolar concentrations of haloperidol, (+)-butaclamol, and flupenthixol block the dopamine response. preincubation in ringer's solution containing increased levels of ca2+ (5-110 mm) decreases the sensitivity of the cells to these antagonists by 1,000-10,000 times. dopamine sensitivity of the cells is not affecte ...19826179080
resonance raman studies of the quaternary structural change in carp deoxy hemoglobin. 19827084471
the primary structure of carp myoglobin in the context of molecular evolution. 19826124014
lateral spread of s-potential components in the carp retina. 19827067747
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