Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| elisa for detection of igg and igm antibodies to hsv-1 and hsv-2 in human sera. | a rapid, enzyme-linked immunoassay (elisa) was applied to identify and measure specific igg and igm antibodies to herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2). detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with hsv-1 or hsv-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates ... | 1986 | 3021796 |
| a novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies. | a novel herpes simplex virus type 1 (hsv-1)-specific glycoprotein reactive with monoclonal antibody h1379 was purified by affinity chromatography. this glycoprotein, provisionally designated as gg-1, forms two sets of bands with molecular weights of 40-44,000 and 60-88,000. when used in an immunodot enzymatic assay, gg-1 reacted strongly with rabbit antisera to hsv-1, but not with sera hyperimmune to hsv-2. specificity of the assay was further established by the lack of reactivity of convalescen ... | 1986 | 3021801 |
| binding of complement component c3b to glycoprotein gc of herpes simplex virus type 1: mapping of gc-binding sites and demonstration of conserved c3b binding in low-passage clinical isolates. | the sites on glycoprotein gc of herpes simplex virus type 1 (hsv-1) which bind complement component c3b were evaluated by using anti-gc monoclonal antibodies and mutants which have alterations at defined regions of the glycoprotein. monoclonal antibodies were incubated with hsv-1-infected cells in a competitive assay to block c3b binding. each of 12 different monoclonals, which recognize the four major antigenic sites of gc, completely inhibited c3b binding. with this approach, no one antigenic ... | 1986 | 3021981 |
| construction and properties of a cell line constitutively expressing the herpes simplex virus glycoprotein b dependent on functional alpha 4 protein synthesis. | we report the construction of a cell line constitutively expressing the glycoprotein b (gb) of herpes simplex virus (hsv) 1. the cell line was constructed in two steps. in the first, a baby hamster kidney cell line was transfected with the dna of a plasmid containing the neomycin phosphotransferase gene that confers resistance to the antibiotic g418 and the gene specifying a temperature-sensitive (ts-) alpha 4 protein of hsv-1, the major viral regulatory protein. a clonal cell line, alpha 4/c113 ... | 1986 | 3022001 |
| sequences homologous to two separate transforming regions of herpes simplex virus dna are linked in two human genital tumors. | ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (hsv-2) dna sequences. two vulvar tumors and one vulvar dysplastic tissue were found to contain dna sequences homologous to the bglii o fragment (coordinates 0.38-0.42) and the bglii n fragment (coordinates 0.58-0.63) of hsv-2 dna. these two fragments overlap the subsets of hsv-1 and hsv-2 dna sequences (respectively) shown previously t ... | 1986 | 3022470 |
| efficacy of 2'-nor-cyclicgmp in treatment of experimental herpes virus infections. | 9-[(2-hydroxy-1,3,2-dioxaphosphorinan-5-yl)oxymethyl]guanine p-oxide (2'-nor-cgmp), the cyclic phosphate of 2'-nor-deoxyguanosine (2'-ndg) was synthesized by phosphorylation of 2'-ndg and evaluated for antiherpetic activity in cell cultures and in animal protection studies. 2'-nor-cgmp was effective in cell culture against both thymidine kinase deficient and wild-type herpes simplex virus type 1 strains and also against herpes simplex virus type 2. the anti-herpes activity of 2'-nor-cgmp against ... | 1986 | 3022643 |
| autonomic nervous system involvement in experimental genital infection by herpes simplex virus type 2. | peroxidase-antiperoxidase technique and histology were employed to elucidate the peripheral routes involved in hsv-2 progression from vagina towards the central nervous system in mice. 12 week-old female balb/c mice were intravaginally infected with 5 x 10(5)ld50 of hsv-2. sixty per cent of animals developed vulvovaginitis, perigenital alopecia and hind-limb paresia. death occurred at 9-11 days post-infection. colon dilatation and urinary bladder distention were observed in all cases. complete t ... | 1986 | 3022683 |
| type specificity of complement-fixing antibody against herpes simplex virus type 2 ag-4 early antigen in patients with asymptomatic infection. | we evaluated the type specificity of complement-fixing (cf) antibody against the ag-4 early antigen of herpes simplex virus (hsv) type 2 (hsv-2) by comparing a commercial ag-4 cf kit (simplex-2; gene link australia, inc., princeton, n.j.) with quantal microneutralization (mn) and absorption-western blotting in testing sera from patients with and without a history of genital herpes. sera characterized as hsv type 1 (hsv-1) or hsv-2 positive or negative by mn were selected and tested by cf, and th ... | 1986 | 3023439 |
| herpes simplex virus type 2 mutagenesis: characterization of mutants induced at the hprt locus of nonpermissive xc cells. | in a previous report, herpes simplex virus type 2 (hsv-2) was shown to increase the frequency of mutation at the hypoxanthine phosphoribosyltransferase (hprt) locus of nonpermissive rat xc cells (l. pilon, a. royal, and y. langelier, j. gen. virol. 66:259-265, 1985). a series of 17 independent mutants were isolated after viral infection together with 12 spontaneous noninfected mutants to characterize the nature of the mutations induced by the virus at the molecular level. the dna of the mutants ... | 1986 | 3023954 |
| viral antibodies in serum and cryoprecipitate of patients with essential mixed and secondary cryoglobulinemia. preliminary results. | antibodies to measles virus, cmv, hsv1, hsv2 and ebv have been tested in 9 patients with essential mixed cryoglobulinemia (emc) and 24 patients with cryoglobulinemia associated with lymphoproliferative, autoimmune or hepatic diseases (secondary cryoglobulinemia, sc). the assays were performed in serum, cryoprecipitate and supernatant by using immunoenzymatic methods. the highest prevalence was observed in the supernatant of both emc and sc patients. antibodies to measles virus show a serum/cryop ... | 1986 | 3024297 |
| the nucleotide sequence of the gb glycoprotein gene of hsv-2 and comparison with the corresponding gene of hsv-1. | the nucleotide sequence of the gb glycoprotein gene of hsv-2 has been determined and compared with the homologous gene of hsv-1. the two genes are specified by the same total number of codons (904); eight additional codons of the hsv-1 gene are found within the signal sequence, and eight additional codons of the hsv-2 gene are found at three different sites in the gene. the signal cleavage, membrane-spanning, and eight potential n-linked oligosaccharide sites, as well as 5'- and 3'-regulatory si ... | 1986 | 3024391 |
| mode of action, toxicity, pharmacokinetics, and efficacy of some new antiherpesvirus guanosine analogs related to buciclovir. | 9-[4-hydroxy-3-(hydroxymethyl)butyl]guanine (3hm-hbg), (rs)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine ([+/-]2hm-hbg), and cis-9-(4-hydroxy-2-butenyl)guanine (2en-hbg), new acyclic guanosine analogs structurally related to buciclovir (bcv [(r)-9-(3,4-dihydroxybutyl)guanine]), were evaluated in parallel with buciclovir as anti-herpes simplex virus (hsv) agents. in cell cultures, replication of different strains of hsv type 1 (hsv-1) and hsv-2 was inhibited at nontoxic drug concentrations. the co ... | 1986 | 3024562 |
| characterization of acyclovir-resistant and -sensitive herpes simplex viruses isolated from a patient with an acquired immune deficiency. | several different genital and non-genital hsv isolates were obtained from a patient with an acquired immune deficiency of unknown aetiology. the patient was initially treated with topical acyclovir (acv) and later with topical and intravenous acv. in spite of treatment with antiviral drugs the patient continued to shed virus and to have extensive genital ulcerations. restriction endonuclease (re) analyses of the viral dna revealed that all the isolates had characteristic hsv-2 patterns and that ... | 1986 | 3025153 |
| anti-herpes virus combinations in relation to drug resistance. | two broad categories of combinations have been subject to experimental investigation against hsv-1 and hsv-2; those consisting of an active antiviral drug and an inhibitor of its catabolism and those which contain two active antiviral agents. the latter seek to exploit biochemical mechanisms likely to lead to a synergistic or complementary interaction. examples of both types of combination for the suppression of resistance development and for the treatment of infection caused by resistant strain ... | 1986 | 3025155 |
| the antiviral activity against herpes simplex virus of the triterpenoid compounds carbenoxolone sodium and cicloxolone sodium. | dose-response experiments show that the presence of 300 microm cicloxolone sodium (ccx) or 500 microm carbenoxolone sodium (cbx) during the hsv replication cycle reduced the infectious virus yield by 10,000- to 100,000-fold: ccx is the more potent anti-herpes agent. hsv-2 replication was consistently more severely restricted by either drug than was that of hsv-1. the ed50 values obtained for either drug against hsv-1 or hsv-2 correlate well with data from dose-response curves. ccx, and to a less ... | 1986 | 3025156 |
| transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus. | herpes simplex virus type 2 causes a severe repression of host cell biosynthesis at a number of levels. we show that despite this, non-viral cdna clones derived from cellular rna species which accumulate to high levels after infection can be isolated using differential screening techniques. by using nuclear run-off assays, we have shown that this rna accumulation is mediated by transcriptional induction of the corresponding cellular genes. | 1986 | 2883998 |
| induction of recurrent genital herpes simplex virus type 2 infection by ultraviolet light. | 1986 | 2870285 | |
| studies on interactions of dtk-hsv mutants with neurons in vitro. | thymidine kinase negative (dtk-) mutants of herpes simplex virus type 1 (hsv-1) multiplied well in rat brain glioma cells. a proportion (less than 1%) of glioma cells survived the infection with hsv and were designated "survivor" glioma cells. survivor cells of dtk- mutant virus infection ceased to produce infectious virus after two passages and were highly resistant to both hsv-1 and hsv-2 but not to vesicular stomatitis virus (vsv). flow cytometric studies indicated morphological differences b ... | 1986 | 2871727 |
| early intrathecal production of specific igm and igg antibodies and alpha-interferon in herpes simplex virus encephalitis. | a complex approach was used in order to establish non-invasively the aetiology in three cases of encephalitis presumptively caused by herpes simplex virus (hsv). as indicative of brain hsv infection was considered the lowered serum to cerebrospinal fluid specific antibody ratio, which also assessed the humoral immune response within the cns. for this purpose, during ongoing brain tissue infection, the early intrathecal (ith) production of igg and igm antibodies was analysed by a differential enz ... | 1986 | 2873733 |
| induction of chromosomal aberrations in human cells by a temperature-sensitive mutant of herpes simplex virus type 2 and its revertants. | the induction of chromosomal aberrations by a temperature-sensitive (ts) mutant of herpes simplex virus type 2 (hsv-2) strain hg52 (ts 13), its revertants 4-8 and 5-8 and by etalon strains hsv-1 17 syn+ and hsv-2 hg52 was studied in human fibroblast and lymphocyte cultures. the effect on chromosomes of the revertants was tested at permissive (31 degrees c) and non-permissive (38 degrees c) temperatures. at 38 degrees c the revertants could not induce dnase activity. the present results contribut ... | 1986 | 2874732 |
| hsv infected raji-cells specify hsv specific immediate early and/or early dna-binding proteins. | herpes simplex virus specified dna-binding proteins were purified from virus infected vero and raji cells. the expression of the proteins differed depending on the type of the host cell. polypeptides corresponding to hsv-1 specific icp 8 and hsv-2 specific icsp 11/12 were the major products of hsv-infected vero cells. in raji cells polypeptides with a corresponding molecular weight, together with a cellular protein having almost similar mobility on sds gels could be detected. using immuno-blotti ... | 1986 | 3002298 |
| immunity to herpes simplex virus type 2: viral antigen-presenting capacity of epidermal cells and its impairment by ultraviolet irradiation. | ia+ epidermal cells (ec) had accessory cell function for herpes simplex virus type 2 (hsv-2)-induced t cell proliferation of immune lymph node cells (lnc). the ec-mediated virus-induced proliferative response of immune lnc was inhibited by ultraviolet b (uvb) irradiation. when ec were pulsed with viral antigen before uvb irradiation, the response was partially restored by addition of epidermal cell-derived thymocyte-activating factor (etaf). although normal ec secreted prostaglandin e2, the leve ... | 1986 | 3001186 |
| mothers of children with down syndrome have higher herpes simplex virus type 2 (hsv-2) antibody levels. | the antibody response to herpes simplex virus (hsv) was studied in 53 mothers of children with down syndrome (ds) and compared with that in 154 controls, using sera sampled during pregnancy or at delivery. conventional analysis of hsv complement fixing antibodies showed the same frequency of positivity for the two groups (70%). when the levels of igg antibodies to an hsv-1 and an hsv-2 antigen preparation were determined by an enzyme-linked immunosorbent assay (elisa) technique, it was found tha ... | 1986 | 3002957 |
| infection of the adrenal gland as a route to the central nervous system after viraemia with herpes simplex virus in the mouse. | intravenous inoculation of 4-week-old female nih (inbred) mice with herpes simplex virus type 1 (hsv-1) strain p2c6 (defective in thymidine kinase) produced bilateral hind limb paralysis in nearly all animals by the 5th day after inoculation; very few mice died. in male mice the incidence and severity of paralysis was considerably lower than in females. the parental strain, cl(101), produced similar paralysis but all mice died by day 7. observations on paralysis and death after intravenous inocu ... | 1986 | 3003239 |
| an in vitro latency system for herpes simplex virus type 2. | an in vitro latency system for herpes simplex virus type 2 (hsv-2) in cultured cells has been developed. virus replication was suppressed by infection of human foetal lung cells at the supraoptimal temperature of 42 degrees c, and, following transfer of such cell cultures to the normal growth temperature of 37 degrees c, infectious virus was generally undetectable for at least 6 days. hsv-2 was reactivated by intertypic superinfection at 38.5 degrees c with temperature-sensitive mutants of hsv-1 ... | 1986 | 3003243 |
| age-specific prevalence of antibodies to pyrimidine kinase enzymes of herpes simplex virus types 1 and 2, and of varicella zoster virus. | the age-specific prevalence of antibodies to pyrimidine kinase enzymes of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) and of varicella zoster virus (vzv) was measured in serum specimens from 360 persons. specific inhibition of viral enzyme activity by the serum was used as an indication of the presence of antibodies to the enzyme. for hsv-1 and hsv-2 together the overall prevalence of positive sera increased with age and reached about 50% in the older age groups, the major part of the pos ... | 1986 | 3003247 |
| hexavalent capsomers of herpes simplex virus type 2: symmetry, shape, dimensions, and oligomeric status. | the structures of the hexavalent capsomers of herpes simplex virus type 2 were analyzed by negative staining electron microscopy of capsomer patches derived from partially disrupted nucleocapsids. optimally computer-averaged images were formed for each of the three classes of capsomer distinguished by their respective positions on the surface of the icosahedral capsid with a triangulation number of 16; in projection, each capsomer exhibited unequivocal sixfold symmetry. according to corresponden ... | 1986 | 3003389 |
| delta 9-tetrahydrocannabinol decreases alpha/beta interferon response to herpes simplex virus type 2 in the b6c3f1 mouse. | this study was undertaken to determine the effect of delta 9-tetrahydrocannabinol (delta 9-thc) on polyinosinic:polycytidylic acid [poly(i):poly(c)]-induced, and on herpes simplex virus type 2 (hsv-2)-induced, alpha/beta interferon in the b6c3f1 mouse. animals were administered delta 9-thc, or the diluent, intraperitoneally for 4 consecutive days or at various time intervals prior to administration of the interferon inducer. poly(i):poly(c) or hsv-2 was injected intravenously on day 4. animals r ... | 1986 | 3003756 |
| typing of herpes simplex virus with synthetic dna probes. | three single-stranded oligonucleotide probes, 22 bases long, homologous to unique regions of herpes simplex virus (hsv) types 1 (hsv-1) and 2 (hsv-2) and a region common to both were chemically synthesized with use of a modified phosphochloridite protocol. for hybridization experiments each probe was labeled with use of polynucleotide kinase and [gamma-32p] atp to a specific activity of approximately 2 x 10(9) cpm/micrograms. two hundred one clinical isolates of hsv (96 hsv-1 and 105 hsv-2) coll ... | 1986 | 3005436 |
| immunological reactivity of human sera with individual herpes simplex proteins: a comparative study of sera from patients with preinvasive or invasive cervical cancer and from controls. | forty-three human sera collected from patients with preinvasive or invasive cervical carcinoma were analyzed for their repertoire of herpes simplex virus (hsv) specific antibodies reactive with individual viral hsv-1 and hsv-2 proteins. the reactivity was compared to that of sera from 27 control persons. the patients and controls were clinically and histologically characterized in a previous study we carried out, where the analysis of the hla-antigen pattern was compared among the groups [vass-s ... | 1986 | 3005489 |
| characterization of the genes encoding herpes simplex virus type 1 and type 2 alkaline exonucleases and overlapping proteins. | a detailed sequence analysis of the herpes simplex virus type 1 (hsv-1) and hsv-2 dna encoding the alkaline exonuclease mrna clusters has been completed. three partially colinear mrnas (2.3, 1.9, and 0.9 kilobases) are completely encoded within the dna sequence presented. the putative promoter regions of the transcripts were inserted upstream of a plasmid-borne chloramphenicol acetyl transferase (cat) gene and assayed for their ability to induce transcription of the cat gene upon low multiplicit ... | 1986 | 3005609 |
| pathogenicity in mice of herpes simplex virus type 2 mutants unable to express glycoprotein c. | herpes simplex virus type 2 (hsv-2) mutants that were unable to express glycoprotein c (gc-2) were isolated. deletions were made in a cloned copy of the gc-2 gene, and recombinant viruses containing these deletions were screened by using an immunoreactive plaque selection protocol. the viruses did not display a syncytial phenotype. intravaginal inoculation of balb/cj mice with one of the hsv-2 gc-2- viruses produced local inflammation followed by a lethal spread of the viral infection into the n ... | 1986 | 3005656 |
| effects of mercury (ii) compounds on the activity of dutpases from various sources. | the deoxyuridine triphosphate nucleotidohydrolases (dutpases, ec 3.6.1.23) from escherichia coli k-12-,acholeplasma laidlawii b-pg9-, human kb cell-, and the herpes simplex virus (hsv) type 1- and 2-induced dutpases were purified and used to determine the effect of various mercury (ii) compounds on their activities. mercuric acetate, 5-mercuri-dutp (hgdutp), and 5-mercuri-dctp (hgdctp) acted as irreversible active site-directed inhibitors of the dutpases purified from eukaryotic organisms but no ... | 1986 | 3005836 |
| infections with herpes simplex viruses (1). | 1986 | 3005858 | |
| dna amplification and neoplastic transformation mediated by a herpes simplex dna fragment containing cell-related sequences. | the transforming potential of the herpes simplex virus type 2 (hsv-2) bamhi fragment e (map position 0.533-0.583) encoding the 140-kda ribonucleotide reductase was assayed by transfection in established rat-2 cells. foci of refractile, morphologically distinguishable cells were induced at lower efficiency and after a longer incubation period as compared to the human tumor oncogene ej-ha-ras. focus-derived bamhi fragment e-transformed cell lines formed medium-to-large (0.1-0.25 mm) colonies in so ... | 1986 | 3006067 |
| identification, properties, and gene location of a novel glycoprotein specified by herpes simplex virus 1. | we report the identification of a novel herpes simplex virus 1 (hsv-1) glycoprotein reactive with type specific monoclonal antibody h1379. the monoclonal antibody reacted with two broad bands with apparent mol wt of 60k to 68k and 44k to 48k formed by infected cell lysates subjected to electrophoresis in denaturing polyacrylamide gels and electrically transferred to a nitrocellulose sheet. early in infection the h1379 reactive protein was found in the faster migrating band. the rate of accumulat ... | 1986 | 3006335 |
| comparison of risk factors for cervical cancer in different populations. | the incidence of cervical cancer has been found to vary between populations. risk factors of cervical cancer include early age at first marriage, multiple marriages and antibodies to herpes simplex virus type 2 (hsv-2). the interrelatedness of these risk factors was examined by comparing data collected from 428 cancer cases and 947 control women selected from 6 populations having standardized cervical cancer incidence rates varying from 9.3 to 85.1 per 100,000. logistic regression analysis revea ... | 1986 | 3007372 |
| characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for n-acetylgalactosamine-specific lectins and its identification as g92k or gg. | extracts from herpes simplex virus type 2 (hsv-2)-infected cells were subjected to affinity chromatography with gel-bound helix pomatia lectin (hpa). only one hsv-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130k). the hpa-binding glycoprotein was genetically mapped, using hsv-1 x hsv-2 intertypic recombinants into the short component of the hsv-2 genome. the mapping position, electrophoretic mobility and the antigenic ... | 1986 | 3007660 |
| novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene us4. | gene us4 of herpes simplex virus type 1 (hsv-1) has been predicted, from dna sequence analysis, to encode a protein of molecular weight 25237 and its properties suggest it to be a membrane-associated protein. we have investigated this protein by raising antiserum to a synthetic oligopeptide corresponding to a stretch of amino acids from an internal hydrophilic region of the predicted sequence. this antiserum immunoprecipitates three glycoprotein species of apparent mol. wt. 37 000, 48 000 and 56 ... | 1986 | 3007661 |
| herpes simplex virus type 1 dna sequences which direct spread of virus from cornea to central nervous system. | the virulence of a herpes simplex virus (hsv) intertypic recombinant possessing hsv-1 dna sequences from map units 0.31 to 0.44 and hsv-2 sequences from map units 0 to 0.30 and 0.45 to 1.0 were compared with the virulence of the two parental strains. following ocular inoculation, both the intertypic recombinant and the hsv-1 parent replicated at the infection site and spread to the peripheral and central nervous system (cns) to produce fatal encephalitis. the hsv-2 parent also replicated at the ... | 1986 | 3008422 |
| efficacy of 5-methoxymethyl-2'-deoxyuridine in combination with arabinosyladenine for the treatment of primary herpes simplex genital infection of mice and guinea pigs. | the relative efficacy of 5-methoxymethyl-2'-deoxyuridine (mmdurd), arabinosyladenine (ara-a) and the combination of mmdurd and ara-a in the treatment of experimental genital herpes (gh) was investigated using mouse and guinea pig models. the infection was initiated by intravaginal inoculation using either hsv-2, strain x-265 or hsv-2, strain ms. treatment was initiated 3 h post virus inoculation. the parameters used to evaluate efficacy were: percent mortality; mean day of death; virus yield fro ... | 1986 | 3008644 |
| antibody response to the herpes simplex virus type 2 ag-4 antigen in guinea pigs with genital herpes infections. | female guinea pigs, inoculated intravaginally with a herpes simplex virus-type 2 (hsv-2) strain m1, developed typical symptoms of a primary and recurrent genital herpes infection. sera from hsv-2 infected guinea pigs taken during the primary or recurrent stages of the genital infection contained complement (c)-fixing antibody which reacted with an apparent type specificity to an early 4 h hsv-2 infected cell extract (ag-4) when compared to a 4 h hsv-1 infected cell extract. this c-fixing anti-ag ... | 1986 | 3008691 |
| intracellular localization and serological identification of a hsv-2 protein in cervical cancer. | exfoliated atypical cells from us and italian patients with cervical intraepithelial neoplasia or invasive cancer stained in indirect immunofluorescence with a monoclonal antibody to a high mw hsv-2 protein (icp10). the proportion of staining cells increased as a function of the severity of the cervical anaplasia. in the majority of cases with mild or moderate dysplasia staining was restricted to the cytoplasm while atypical cells from 6 of 7 cases with marked dysplasia and 7 of 7 cases with inv ... | 1986 | 3009194 |
| genital reinfection after recovery from initial genital infection with herpes simplex virus type 2 in guinea pigs. | the natural history of genital reinfection with herpes simplex virus type 2 (hsv-2) three weeks to one year after initial infection was explored by using a guinea pig model. intravaginal reinoculation with hsv-2 of animals that had recovered from initial genital infection produced by wild-type or thymidine kinase-deficient hsv-2 produced asymptomatic genital reinfection characterized by high-titered replication of hsv-2 in the vaginal vault. reinoculation did not result in acute neural infection ... | 1986 | 3009643 |
| synthesis and antiviral properties of 5-(2-substituted vinyl)-6-aza-2'-deoxyuridines. | the following 5-(2-substituted vinyl)-6-aza-2'-deoxyuridines were synthesized: (e)-5-(2-bromovinyl) (2) (6-aza-bvdu), 5-(2-bromo-2-fluorovinyl) (a mixture of e and z isomers) (3), (e)-5-(2-chlorovinyl) (4), (e)-5-[2-(methylthio)vinyl] (5), 5-(2,2-dibromovinyl) (6), and 5-(3-furyl) (7). the synthesis of 2-6 utilized wittig-type reactions on 5-formyl-1-(2'-deoxy-3', 5'-di-o-p-toluoyl-beta-d-erythro-pentofuranosyl)-6-azauracil (16). 6-aza-bvdu (and its alpha-anomer) was also synthesized from (e)-5- ... | 1986 | 3009815 |
| cloning and characterization of oril2, a large palindromic dna replication origin of herpes simplex virus type 2. | an origin of replication within the long unique sequence of herpes simplex virus type 2 designated oril2 has been identified in a position homologous to its type 1 counterpart, oril1, between map coordinates 0.398 and 0.413. the difficulties encountered in previous attempts to clone both oril2 and oril1 in an undeleted form were surmounted by minimizing the growth of the host escherichia coli, using a recbc sbcb e. coli host, and purifying the full-length plasmid from delected forms by using a n ... | 1986 | 3009865 |
| expression of herpes simplex virus glycoproteins in polarized epithelial cells. | members of the herpesvirus family mature at inner nuclear membranes, although a fraction of the viral glycoproteins is expressed on the cell surface. in this study, we investigated the localization of herpes simplex virus type 2 (hsv-2) glycoproteins in virus-infected epithelial cells by using a panel of monoclonal antibodies directed against each of the major viral glycoproteins. all of the hsv-2 glycoproteins were localized exclusively on the basolateral membranes of vero c1008, madin-darby bo ... | 1986 | 3009881 |
| identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the l component of herpes simplex virus genome. | in the course of studies on the a sequences located at the termini of and at the junction between the l and s components of herpes simplex virus 1 dna, j. chou and b. roizman (j. virol. 57:629-637, 1986) noted that the a sequence acted as a gamma 1 promoter when fused to the structural sequence of the thymidine kinase gene, the b inverted repeat sequences located in the l component next to the a sequences contained an open reading frame predicted to encode the protein of 358 amino acids with a m ... | 1986 | 3009891 |
| effect of delta 9-tetrahydrocannabinol on herpes simplex virus type 2 vaginal infection in the guinea pig. | the present investigation was undertaken to determine whether delta 9-tetrahydrocannabinol (delta 9-thc) decreases host resistance to herpes simplex virus type 2 vaginal infection in the guinea pig. the guinea pig was selected as the host since it has been shown to express a spectrum of primary herpes genitalis which is similar to that in humans. animals were administered delta 9-thc or vehicle intraperitoneally on days 1-4, 8-11, and 15-18. herpes simplex virus was introduced intravaginally on ... | 1986 | 3010327 |
| recurrent genital herpes and suppressive oral acyclovir therapy. relation between clinical outcome and in-vitro drug sensitivity. | to evaluate the association between in-vitro resistance of herpes simplex virus type 2 to acyclovir and breakthrough recurrences of herpes despite chronic suppressive therapy, we determined the in-vitro sensitivity of herpes simplex virus isolated before, during, and after therapy. one hundred eighty-three virus isolates from 107 patients were tested. before therapy, the median amount of drug required to inhibit 50% of the virus in tissue culture (id50) was 0.91 microgram/ml. the median id50 aft ... | 1986 | 3010782 |
| molecular cloning of herpes simplex virus type 2 dna. | restriction enzyme hindiii digestion of the whole genome of herpes simplex virus type 2 strain 186 yielded 10 dna fragments with molecular weights ranging from approximately 22 x 10(6) to 1.2 x 10(6), which were cloned into the hindiii site of bacterial plasmid pacyc 184. the cloned fragments were identified by hybridization to hsv-2 virus dna and by double digestion with restriction endonucleases. the recombinant plasmids, even if they carried dna sequences with molecular weights of more than 1 ... | 1986 | 3011764 |
| acute genital infection in guinea pigs: effect of recombinant interleukin-2 on herpes simplex virus type 2. | human recombinant interleukin-2 (ril-2) modifies infection with herpes simplex virus type 2 (hsv-2) in normal guinea pigs. animals were injected sc with ril-2 twice a day, beginning 24 hr before infection and continuing for three subsequent days. guinea pigs were assigned to five different regimens in which ril-2 was administered daily at dosages from 8 x 10(3) u/kg to 8 x 10(5) u/kg. after intravaginal inoculation with hsv-2, 83% of 24 control animals developed apparent genital herpes, and 5% h ... | 1986 | 3011918 |
| suppression and enhancement of humoral antibody formation by herpes simplex virus types 1 and 2. | intraperitoneal infection of mice and rats by herpes simplex virus type 2 (hsv-2) but not type 1 (hsv-1) resulted in suppression of antibody formation on subsequent challenge with hsv-1 or hsv-2. application of silica considerably enhanced antibody formation after primary hsv-1 infection, but only slightly after primary hsv-2 infection. suppression induced by hsv-2 was, however, reduced significantly by injection of silica 21 days later, on the day of the second injection of hsv-2. suppression c ... | 1986 | 3011967 |
| x-linkage of the early in vitro alpha/beta interferon response of mouse peritoneal macrophages to herpes simplex virus type 2. | the genetics of the early interferon response of mouse peritoneal cells to infection with herpes simplex virus type 2 (hsv-2) was studied in susceptible balb/c and more resistant c57bl/6 mice and in reciprocal crosses between these mice. wash-outs of the peritoneal cavity of normal c57bl/6 mice contained significantly more cells than wash-outs from balb/c mice. therefore, interferon induction with hsv-2 was studied under standardized conditions in vitro. peritoneal cells reacted to hsv-2 infecti ... | 1986 | 3011968 |
| the herpes simplex virus type 2 alkaline dnase activity is essential for replication and growth. | a mutant of herpes simplex virus type 2 (hsv-2), which is temperature-sensitive (ts) for the induction of an alkaline dnase activity, was examined at a number of different temperatures. induction of dnase activity by this mutant resembled that of wild-type (wt) virus at 31 degrees c but was greatly reduced at 38.5 degrees c and barely detectable at 39.2 degrees c. virus dna synthesis showed similar patterns, exhibiting wt levels at 31 degrees c, reduced levels at 38.5 degrees c and very little i ... | 1986 | 3011973 |
| inhibition of herpes simplex virus-induced dna polymerases and cellular dna polymerase alpha by triphosphates of acyclic guanosine analogs. | the triphosphates of the antiherpes acyclic guanosine analogs (r)- and (s)-enantiomers of 9-(3,4-dihydroxybutyl)guanine [bcvtp and (s)-dhbgtp], 9-(4-hydroxybutyl)guanine (hbgtp), and 9-(2-hydroxyethoxymethyl)guanine (acvtp) were investigated for their effects on partially purified dna polymerases of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) as well as cellular dna polymerase alpha of calf thymus and vero cells. the triphosphates of the four analogs were all competitive inhibitors ... | 1986 | 3012321 |
| specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides. | ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ... | 1986 | 3012359 |
| analysis of dna from recurrent genital herpes simplex virus isolates by restriction endonuclease digestion. | in a blind study, dna from 40 clinical isolates of herpes simplex viruses was analyzed by restriction endonucleases to determine whether serial isolates from an individual patient could be identified and whether exogenous reinfection occurred within this population. five of the 40 isolates served as controls. based on restriction patterns obtained following bam h1 cleavage of dna, 35 isolates were assigned to 15 patients. isolates from two patients displayed variation in the electrophoretic mobi ... | 1986 | 3012807 |
| cellular immunity to vaccinations and herpesvirus infections after bone marrow transplantation. | the cellular immune response to herpesviruses was studied in 46 recipients of marrow grafts (23 autologous, 23 allogeneic). that study was performed in vitro by evaluating the degree of lymphocyte proliferative responses to herpes simplex virus (hsv), cytomegalovirus (cmv), and varicella zoster virus (vzv). no primary infections with any of those viruses were noted after bone marrow transplantation (bmt). the incidence of active infection in seropositive patients was significantly lower after au ... | 1986 | 3012833 |
| efficacy of the acyclic guanosine analog buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] in experimental genital herpes. | the efficacy of the anti-herpesvirus drug buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] was investigated in guinea pigs and mice infected intravaginally with herpes simplex virus type 2. topical treatment initiated early after infection was efficacious, in contrast to topical treatment delayed 24 h or more. systemic treatment of infected mice could not prevent the spread of virus to the brain and mortality. systemically administered buciclovir had an effect in guinea pigs, even after delayed on ... | 1986 | 3013082 |
| comparison of susceptibilities of varicella-zoster virus and herpes simplex viruses to nucleoside analogs. | the susceptibilities of varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 17 nucleoside analogs were compared by a plaque reduction assay with human embryonic lung fibroblast cells. the susceptibility of vzv to certain nucleoside analogs was different from that of hsv-1. against vzv the 5-halogenovinyl-arabinosyluracils were the most potent of the compounds tested. | 1986 | 3013088 |
| evaluation of five cell types for the isolation of herpes simplex virus. | five cells were evaluated in a comparative analysis for sensitivity, specificity, and rapidity in detecting the presence of herpes simplex virus hsv-1 and hsv-2. included in this study were human embryonic kidney (hek), rabbit kidney (rk), mrc-5, mink lung (ml), and microtus agrestis (umma). a total of 274 specimens from genital, throat, skin, or other sources that were submitted for hsv isolation were used in the study. the sensitivity of the different cells was assessed by the total number of ... | 1986 | 3013496 |
| rapid identification of herpesvirus simiae (b virus) dna from clinical isolates in nonhuman primate colonies. | a rapid, simple technique, based on restriction endonuclease analysis of radioactively labeled infected cell dna, is described for identification of herpesvirus simiae (b virus) infection in clinical isolates from nonhuman primates. isolates can be screened within 2-3 days from the time of collection of a specimen from a suspect lesion to final viral identification. isolates were obtained from eight animals with suspected b virus infections. the results indicated the presence of b virus in each ... | 1986 | 3013916 |
| a filamentous distribution for the herpes simplex virus type 2-encoded major dna-binding protein. | monoclonal antibodies reacting with the herpes simplex virus (hsv)-encoded major dna-binding protein defined an intracellular filamentous network. this network was associated predominantly with the infected cell nucleus and occurred in cells infected with hsv type 2. it did not co-distribute with microfilaments, microtubules or intermediate filaments, and dna synthesis was required for its formation. we suggest explanations for the occurrence and function of this novel filamentous network struct ... | 1986 | 3014046 |
| characterization of the tumor-associated 38-kd protein of herpes simplex virus type 2. | the bglii n dna fragment of herpes simplex virus type 2 (hsv 2), which is capable of oncogenically transforming cells in vitro, encodes a 37,800-dalton (38-kd) protein that has been seroepidemiologically associated with uterine cervical carcinoma. polyclonal monospecific antiserum was produced against electrophoretically purified 38 kd from hsv 2-infected cells and used to identify antigenic and biochemical characteristics of the protein as well as to probe transformed cells for the expression o ... | 1986 | 3014138 |
| comparative studies of host-cell reactivation, cellular capacity and enhanced reactivation of herpes simplex virus in normal, xeroderma pigmentosum and cockayne syndrome fibroblasts. | host-cell reactivation (hcr) of uv-irradiated herpes simplex virus type 2 (hsv-2), capacity of uv-irradiated cells to support hsv-2 plaque formation and uv-enhanced reactivation (uver) of uv-irradiated hsv-2 were examined in fibroblasts from 4 patients with cockayne syndrome (cs), 5 with xeroderma pigmentosum and 5 normals. all uv-survival curves for hsv-2 plaque formation showed 2 components. hcr was similar to normal for the xp variant strain and the 2 cs strains tested, but substantially redu ... | 1986 | 3014327 |
| [comparative study of the effectiveness of acycloguanosine and phosphonoacetic acid in herpetic infection in cell cultures]. | a comparative study of cytotoxicity and antiherpes activity of acycloguanosine (acg) of the soviet and american manufacture and a soviet preparation of phosphonoacetic acid (paa) for herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was carried out in primary and continuous cell cultures. the acg preparation was shown not to be inferior in maximal tolerated concentration, minimal inhibiting concentration, and chemotherapeutic index (cti) to american drug. zovirax (zvr), in relation to both he ... | 1986 | 3014751 |
| activities of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine and its metabolites against herpes simplex virus types 1 and 2 in cell culture and in mice infected intracerebrally with herpes simplex virus type 2. | as measured by plaque and yield reduction assays, several metabolites of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac) were highly active against herpes simplex virus types 1 and 2. these metabolites included the 2'-deoxy-2'-fluoroarabinosyl derivatives of 5-iodouracil (fiau), cytosine (fac), uracil (fau), and thymine (fmau). in mice inoculated intracerebrally with herpes simplex virus type 2, the relative order of potency of these compounds and licensed antiviral drugs was ... | 1986 | 3015003 |
| effect of interferon on herpes simplex virus replication in murine macrophage-like cell lines. | the effect of interferon on the replication of herpes simplex virus types 1 and 2 was studied in two murine macrophage-like cell lines which differ in their ability to synthesize interferon. higher titers of herpes simplex virus type 1 occurred in pu5-1.8 cell cultures where interferon was not produced than in j774a.1 cell cultures where low amounts of interferon were produced. herpes simplex virus type 2 replicated poorly in both types of cell cultures. interferon synthesis was induced in j774a ... | 1986 | 3015020 |
| herpes simplex virus type 1 and 2 in the adrenal glands: replication and histopathology. | the adrenal glands were shown to be the most severely infected organs in the early phase of hsv-1 infections (up to 10 days p.i.) after i.p. infections in mice. virus could be isolated from the adrenal glands as early as one hour after infection with pathogenic and apathogenic strains. infection of the adrenal glands is a result of viremia. the content of hsv-1 (5 strains) was much higher in the adrenals than in spleen and liver. it peaked at 3-4 days p.i. compared to 1-2 days in spleen and live ... | 1986 | 3015081 |
| a latent infection of herpes simplex virus type 2 in a human neuroblastoma cell line imr-32. | human neuroblastoma (imr-32) cells were infected with herpes simplex virus type 2 (hsv-2) at a multiplicity of infection (moi) of 2 plaque-forming units (pfu)/cell and were cultured at 40 degrees c for 14 days. then neither infectious virus particles nor virus capsids were detected in these cells whereas the presence of virus-specific antigens was observed by immunofluorescent antibody staining technique in 16.9 +/- 3.2 per cent of the infected cell population. when the cultivation temperature w ... | 1986 | 3015082 |
| transformation of dog embryo kidney cells by human herpesviruses. | the infection of dog embryo kidney (dek) cells with herpes simplex virus type 2 (hsv-2) or human cytomegalovirus (hcmv) led to the development of transformed cell lines. rapidly dividing dek cells with unlimited division potential exhibited growth in 2% serum, contained nuclear virus antigens, and formed small (+/- 0.2 mm) colonies in 0.3% agarose. immortal cell lines showing the same transformation properties were also obtained after transfection with purified hsv-2 or hcmv dna. these results c ... | 1986 | 3015422 |
| prevalence of htlv iii/lav and other viral antibodies in normal and at risk populations. | the prevalence of htlv iii/lav antibody has been studied in six groups of population: healthy subjects, a virological laboratory staff, prisoners, drug addicts, patients with lymphoadenopathy-associated syndrome (las) and patients with aids. prevalences and levels in the same groups of hbsag and cmv, hsv 1 and hsv 2 antibodies have been studied, too. as expected, the prevalence of htlv iii/lav antibody increases in the six groups with the increasing of the risk while the prevalences of the other ... | 1986 | 3030363 |
| evidence that neomycin inhibits hsv 1 infection of bhk cells. | the effect of neomycin on the herpes simplex virus (hsv) type 1 and 2 infection of baby hamster kidney cells was studied. neomycin concentrations of 3 mm or more caused a more than 90% inhibition of hsv 1 proliferation, while it had no effect on hsv 2 proliferation, measured as plaque-forming units. furthermore, neomycin must be present at the time of infection in order to exert full effect, addition 1 hour postinfection was comparable to untreated cells. this indicates that neomycin may specifi ... | 1986 | 3026374 |
| oral cancer induced in hamsters with herpes simplex infection and simulated snuff dipping. | a number of epidemiologic studies indicate that snuff dipping is associated with an increased incidence of oral cancer in human beings. since inactivated herpes simplex virus (hsv) has been shown to induce malignant changes in vitro and in vivo and is partially inactivated by snuff water extract, we examined the histopathologic changes of hamster buccal pouches after exposure to repeated hsv inoculation combined with long-term simulated snuff dipping. one hundred twenty-five syrian hamsters were ... | 1986 | 3462612 |
| oral bromovinyldeoxyuridine therapy for herpes simplex and varicella-zoster virus infections in severely immunosuppressed patients: a preliminary clinical trial. | twenty-five patients with haematological diseases were treated orally with the highly potent and selective anti-herpes agent, bromovinyldeoxyuridine (bvdu), in a dosage of 7.5 mg/kg/day (divided over three or four doses a day) for 5 days for an intercurrent mucocutaneous herpesvirus infection. of these 25 patients, 8 were severely granulocytopenic at the time of the viral infection, and 12 recently had undergone bone-marrow transplantation; 5 were under cytotoxic therapy for a lymphoproliferativ ... | 1986 | 3511181 |
| a placebo-controlled trial of topical 8% arildone cream early in recurrent genital herpes. | arildone is an aryl diketone which is inhibitory in vitro against herpes simplex virus type 2 at a concentration of 2 micrograms/ml or less. one hundred forty-five patients with recurrent genital herpes were enrolled in a multicenter, randomized, placebo-controlled, double-blind trial to evaluate the efficacy and safety of an 8% arildone cream. patients initiated therapy a mean of 9.9 h and a maximum of 24 h after the reported onset of lesions and applied medication 6 times daily for 7 days. the ... | 1986 | 3521487 |
| [group b streptococcal panophthalmia in neonatal infection]. | hemolyzing streptococci of group b were isolated from an ulcerative keratitis in a 14-day-old girl (gestational age: 39 weeks, birth weight: 3230 g) suffering from septicemia. the differential diagnosis includes all bacteria and virus species able to penetrate the intact cornea, e.g. pseudomonas sp., n. gonorrhoeae and herpes simplex virus ii. perinatal infection may be via the birth canal. | 1986 | 3537493 |
| incidence of herpes genitalis in northern ireland in 1973-83 and herpes simplex types 1 and 2 isolated in 1982-4. | during 1973 - 83 there was a pronounced rise in the number of new cases of herpes genitalis in northern ireland. this study of 127 patients with herpes genitalis shows that 30% had herpes simplex virus type 1 (hsv 1) and 70% had herpes simplex virus type 2 (hsv 2) infections. of 41 sexual contacts who attended, only seven had herpes genitalis (caused in each case by hsv 2); another patient had a history of genital lesions, and one other of oral lesions, but hsv was not isolated from either patie ... | 1986 | 3949348 |
| congenital herpes simplex retinitis. | an 1,810-g girl born at 37 weeks of gestation had true congenital herpes simplex virus type 2 infection. the neonate was born with hypo-pigmented skin lesions, brain lesions, and old heavily pigmented retinal scars in the posterior pole. there was no active ocular disease. viral cultures, immunoperoxidase stains, and antibody determination by enzyme-linked immunosorbent assay were all positive for herpes simplex virus. treatment with acyclovir healed the lesions within ten days. the clinical and ... | 1986 | 3728622 |
| role of virus-infected mononuclear leukocytes in herpetic chorioretinitis of newborn rabbits. | the role of virus-infected mononuclear leukocytes (mnls) in the pathogenesis of neonatal herpetic chorioretinitis in newborn rabbits was investigated. as early as 2 days after inoculating the animals' skins with type 2 herpes simplex virus (hsv-2), infectious mnls in the infected animals' peripheral blood were found. the virus was associated, for the most part, with mnls that belonged to phagocytic and adherent cell fractions. observations by electron microscopy indicated that hsv-2 was actively ... | 1986 | 3759364 |
| delayed treatment with combinations of antiviral drugs in mice infected with herpes simplex virus and application of the median effect method of analysis. | mice were inoculated intracerebrally with a lethal dose of herpes simplex virus type 2. three days later, the mice were treated intraperitoneally, twice daily for 4 days, with the following drugs alone or in combination: acyclovir (acv), vidarabine (ara-a), 2'-fluoro-5-iodoaracytosine (fiac), and 2'-fluoro-5-methylarauracil (fmau). despite delayed treatment, most of the animals receiving low doses of fmau alone or in combination with acv or ara-a survived. in contrast, significantly higher morta ... | 1986 | 3777913 |
| xylotubercidin against herpes simplex virus type 2 in mice. | of a series of newly synthesized derivatives of the pyrrolo[2,3-d]pyrimidine nucleosides tubercidin, toyocamycin, and sangivamycin, the xylosyl analog of tubercidin, xylotubercidin, exhibited the greatest potency and selectivity against herpes simplex virus type 2 (hsv-2) in vitro. at dosage regimens that were not toxic for the host, xylotubercidin proved efficacious in various hsv-2 animal model infections. when applied topically at 0.25, 0.5, or 1% in dimethyl sulfoxide or when administered sy ... | 1986 | 3800347 |
| effect of antibody to interferon on genital herpesvirus infection in mice. | the effect of anti-mouse interferon (ifn) antibody on the course of genital herpes infection was studied in c57b1/6j mice. as we have previously described, intravaginal inoculation of c57 mice with herpes simplex virus type 2 (hsv-2) results in the development of genital lesions, subsequent encephalitis and death, with the mortality rate dependent on virus dose. in the present study, we found that intravaginal application of 1 x 10(5) units of anti-mouse (alpha/beta) ifn antibody did not signifi ... | 1986 | 3508484 |
| central nervous system and genital infection with reactivation of latent herpes simplex virus type 2 in mice. | to establish a reactivation model of genital and central nervous system infection, 3- to 12-week-old outbred or balb/c mice were inoculated vaginally with the hg-52 strain of herpes simplex virus type 2 (hsv-2). primary infection was confirmed by serially positive vaginal cultures. mortality in 6- and 12-week old infected mice was about 20%. in survivors, clearance of infectious virus was confirmed in serially negative vaginal cultures. at 6 weeks, immunosuppression of survivors with cyclophosph ... | 1986 | 3508488 |
| a comparison of imaging, clinical, and pathologic aspects of space-occupying lesions within the brain in patients with acquired immune deficiency syndrome. | patients with acquired immune deficiency syndrome (aids) often have central nervous system mass lesions. since clinical signs, symptoms, and results of imaging techniques are generally nonspecific, brain biopsy has been considered to be the optimal method for establishing a definitive diagnosis. in an effort to define better the imaging characteristics of such lesions, we retrospectively analyzed 24 patients with aids who had intracerebral space-occupying lesions, with subsequent definitive hist ... | 1986 | 3451758 |
| viral meningitis. | viral meningitis is part of the aseptic meningitis syndrome but must be distinguished from bacterial meningitis on the basis of a careful examination of the csf and sound clinical judgment. enteroviruses probably account for the bulk of cases of aseptic meningitis that occur in the united states and which are reported to the centers for disease control each year. the seasonal pattern in the incidence of aseptic meningitis is largely due to the seasonal variation of enteroviral infections. early ... | 1985 | 3990441 |
| cigarette smoking, herpes simplex virus type 2 infection, and cervical abnormalities. | in a case-control study, we investigated the relation of cigarette smoking and genital herpes virus infection to cervical abnormalities. although cases and controls differed according to several factors, multiple logistic analyses indicated that age, education, and cigarette smoking were the only independent risk factors for histologically confirmed disease. relative risk estimates were 1.92 and 3.68 for past and current smokers, respectively. the results indicated that smoking and genital herpe ... | 1985 | 4003639 |
| nucleosides. 133. synthesis of 5-alkenyl-1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines and related pyrimidine nucleosides as potential antiviral agents. | the synthesis of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines with a halovinyl or vinyl substituent at c-5 was accomplished from the corresponding 5-iodo (fiac, 1) and/or 5-chloromercuri nucleoside analogues with use of li2pdcl4- and pd(oac)2-mediated coupling reactions. thiation of the benzoylated derivative of the 5-ethyluracil nucleoside 3 followed by s-methylation and then ammonolysis provided 5-ethyl-2'-fluoro-ara-c. 5-ethynyl-2'-fluoro-ara-c (19a) and 5-ethynyl-2'-fluoro-ara-u (19 ... | 1985 | 4009596 |
| degradation of cellular mrnas induced by a virion-associated factor during herpes simplex virus infection of vero cells. | we have used northern blot hybridization to study the accumulation of specific cellular mrnas in vero cells infected with herpes simplex virus (hsv) type 1 or type 2. hsv-1 infection decreased the cytoplasmic levels of beta- and gamma-actin, beta-tubulin, and histone h3 and h4 mrnas, though not all at the same rate. hsv-2 infection resulted in a more rapid decrease in actin and histone mrna levels compared with hsv-1 infection. the turnover rate of each type of mrna studied was accelerated in hs ... | 1985 | 4020960 |
| oligosaccharide chains of herpes simplex virus type 2 glycoprotein gg.2. | gg.2 glycoprotein was purified by h966 monoclonal antibodies linked to sepharose from herpes simplex virus type 2-infected hep-2 cells labeled with [3h] glucosamine. the glycoprotein was subjected to pronase digestion and the glycopeptides were fractionated by con a-sepharose in a major fraction (88.5% of total radioactivity) unbound to the lectin gel and in a minor species which bound to the lectin as a n-linked diantennary oligosaccharide. mild and strong acid hydrolysis of con a-unbound and c ... | 1985 | 4026310 |
| synergistic antiviral effects of acyclovir and vidarabine on herpes simplex infection in newborn mice. | 277 5-7-day-old white swiss mice were infected intranasally with herpes simplex virus type 2 (hsv2) to mimic disseminated herpetic infection in human newborns. the antiviral efficacy of acyclovir, vidarabine and specific antiserum (sas) alone and in combination was assessed. a significant increase in survival rate (52%) was observed with the combination of acyclovir (80 mg/kg/day) and vidarabine (125 mg/kg/day) compared to either compound (24 and 6%, respectively) when therapy was begun 30 h aft ... | 1985 | 4028874 |
| [herpes simplex virus infections]. | there are two serological types of herpes simplex virus, hsv-1 and hsv-2. the types can be differentiated by different methods. after a primary infection hsv reaches the sensory ganglions by the way of peripheral nervous tracts. the virus remains for life in the ganglion as virus genome. after activation and return movement a new infection can appear. a lot of various diseases are caused by primary infection, reactivation or reinfection. the diagnosis in herpes virus diseases includes the detect ... | 1985 | 4039869 |
| the effect of prostaglandin e2 on the initial immune response to herpes simplex virus infection. | numerous clinical situations, demonstrated to be associated with reactivation of herpes simplex virus infections, have also been shown to produce increases in local levels of prostaglandins. the current study was initiated to determine if prostaglandins play a role in the immune response and control of herpes simplex virus infections. lymphocytes from volunteers were stimulated with concanavalin a, herpes simplex virus 1, or herpes simplex virus 2 in the presence of prostaglandin e2 or ibuprofen ... | 1985 | 3872073 |
| human monocyte-mediated cytotoxicity against herpes simplex virus-infected cells: activation of cytotoxic monocytes by free and liposome-encapsulated lymphokines. | human peripheral blood monocytes were incubated with free or liposome-encapsulated human lymphokines containing macrophage-activating factor (maf) and tested for their effect on herpes simplex virus (hsv)-infected target cells. activated monocytes lysed allogeneic hsv type 2 (hsv-2)-infected whole human embryo cells and xenogeneic balb/c mouse embryo cells (10e2) without any significant effect on uninfected cells, as measured by release of 51cr from target cells after 18 h of cocultivation. kine ... | 1985 | 3882870 |
| the effect of dimethylnitrosamine on host resistance and immunity. | adult female b6c3f1 mice were injected ip with 0.2 ml phosphate-buffered saline (pbs) only or pbs containing 1.5, 3, or 5 mg dimethylnitrosamine (dmn)/kg body wt daily for 14 days. on day 16, mice were evaluated for changes in immune status as measured by the antibody response to sheep red blood cells (srbcs), blastogenesis to t- and b-cell mitogens, natural killer (nk) cell function, delayed hypersensitivity, and alveolar macrophage (am) bactericidal activity; and for changes in host resistance ... | 1985 | 3883572 |
| [discovery of herpes simplex virus (hsv)-like particles in biopsy samples of cervical carcinoma]. | the tissue of cervical cancer and hsv-2 infected baby rabbit kidney (brk) cells were studied comparatively by electron microscope. in 2 out of 12 samples from patients with cervical carcinoma, some virus-like particles were observed in the cytoplasm or intercellular space. some particles seemed to be enveloped by endoplasmic reticulum. according to their size, structure and morphology, these particles resembled the herpes simplex virus. the relationship between the discovery of herpes simplex vi ... | 1985 | 3015529 |
| plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates. | a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ... | 1985 | 3010836 |
| selective in vitro and in vivo activities of 5-(2-haloalkyl)pyrimidine nucleoside analogs, particularly 5-(2-chloroethyl)-2'-deoxyuridine, against herpes simplex virus. | 5-(2-chloroethyl)-2'-deoxyuridine (cedu), 5-(3-chloropropyl)-2'-deoxyuridine (cpdu), and 5-(2-chloroethyl)-2'-deoxycytidine (cedc) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro. their mics for hsv-1 in primary rabbit kidney cell cultures were 0.15, 0.20, and 0.60 micrograms/ml, respectively; their mics for hsv-2 were about 10-fold higher. when tested in parallel, the reference compounds 5-ethyl-2'-deoxyuridine, 5-iodo-2'-deoxyuridine, acyclovir, and ( ... | 1985 | 3010841 |
| mechanism of selective inhibition of human cytomegalovirus replication by 1-beta-d-arabinofuranosyl-5-fluorouracil. | four kinds of 1-beta-d-arabinofuranosyl-5-halogenouracil were examined for inhibition of human cytomegalovirus (hcmv) and herpes simplex virus type 1 (hsv-1) and 2 (hsv-2) replication. 1-beta-d-arabinofuranosyl-5-fluorouracil (ara-fu) was the most effective against hcmv, whereas 1-beta-d-arabinofuranosyl-5-bromouracil was the most effective against hsv-1 and hsv-2. the mechanism of action of ara-fu on hcmv replication was also studied. the dttp pool size in human embryonic fibroblasts was increa ... | 1985 | 3010844 |