Publications

heterogeneity of mitogen-responsive lymphocytes in carp (cyprinus carpio).	peripheral blood leukocytes (pbl) of carp respond in vitro to a variety of phytomitogens, shown to be t-cell specific or b-cell specific in mammalian systems. some basic differences have been observed in the proliferative response of carp pbl to pha (phytohemagglutinin), cona ( concanvalin a) and lps (lipopolysaccharide): (1) the response to pha and cona was found to be highly dependent upon the continuous presence of mitogen in the medium, in contrast to lps, where after the initial stimulation ...	1984	6724093
color fundamentals deduced from carp ganglion cell responses.	retinal ganglion cell responses were used to derive the action spectra (fundamentals) of the photopic color mechanisms in carp. both silencing and chromatic adaptation techniques were used. the former proved to be more successful than the latter, because weak (even subthreshold) dynamic signals in one mechanism (mostly the red) caused strong effects on the other mechanisms. with chromatic adaptation these interacting signals were unavoidable, whereas with the silencing technique the modulation o ...	1984	6719835
ultrastructural evidence for a possible secretory function of merkel cells in the barbels of a teleost fish, cyprinus carpio.	examination of barbels of the carp (cyprinus carpio) revealed cells showing the characteristics of merkel cells. some ultrastructural features of these cells suggest a secretory function.	1984	6713497
fine structure of monoamine-containing basal cells in the taste buds on the barbels of three species of teleosts.	the taste buds on the barbels in three species of teleosts (cyprinus carpio, misgurnus anguillicaudatus, parasilurus asotus) were studied by means of fluorescence- and electron microscopy. intensely yellow-fluorescent cells, which are disk-shaped and located exclusively in a basal position, are observed in the barbel-buds of all fishes examined. the basal cells contain a large number of small clear vesicles approximately 40-60 nm in diameter, which show a tendency to aggregate in the cytoplasm f ...	1984	6713479
[effects of temperature on the horizontal cell potentials (s-potentials) in the perfused isolated retina of carp].		1984	6711402
evidence for the existence of an il-2-like lymphocyte growth promoting factor in a bony fish, cyprinus carpio.	activity promoting the growth of carp t-like cells has been found in supernatants of mitogen (pha)- and alloantigen (mlr)-stimulated carp leukocyte cultures. activity level in culture supernatants was elevated by phorbol myristate acetate. proliferation of carp t-like lymphoblasts was also promoted in the presence of il-2-containing supernatants of mammalian origin. the significance of these findings is discussed.	1984	6609852
[on the existence of 2 neuronal populations in the nucleus rotundus of the carp, cyprinus carpio].		1984	6545980
receptive field properties of the photopic luminosity horizontal cell of carp retina.	the receptive field of the lehc of the carp's retina is different when tested with red versus green stimuli. the sensitivities to 706 nm vs 519 nm flashes were compared for various size spots centered on the receptive field. full summation (area times intensity equaling a constant at threshold) and greater than full summation were found to occur up to larger diameters of spots with red illumination than with green. a further test was made of the effect of constant background green (502 nm) illum ...	1984	6534018
linear color opponency in carp retinal ganglion cells.	double opponent (i.e. red/green cone opponent in center and surround of the receptive field) ganglion cells in photopic carp retina were studied. it was found that a subclass of these cells (about 30%) was linearly opponent. for these cells the response to stimulation of the receptive field center could be constructed by summation of the responses due to stimulation of the red and green cone components separately. these linear opponent cells had smaller receptive field sizes than the nonlinear d ...	1984	6534009
adaptation of fatty acid composition to temperature--a study on carp (cyprinus carpio l.) liver slices.	liver slices obtained from warm-, and cold-adapted carp were incubated in the presence of [1-14c]sodium acetate, -stearate, -linoleate, and -linolenate at various temperatures and the distribution of radioactivity among different phospholipid fatty acids was determined. relative labelling of saturated fatty acids is reduced, while that of long chain polyunsaturated fatty acids, especially of docosahexanoate, is increased with decreasing temperatures. liver slices of cold adapted carp produced a ...	1984	6518757
functional morphology and histology of the olfactory organs of the hill stream carp crossocheilus latius latius (hamilton).		1984	6489877
the hypothalamo-hypophysial system of the wild carp, cyprinus carpio l. i. structure and ultrastructure of the posterior neurohypophysis.	the posterior neurohypophysis (pnh)-pars intermedia complex of the wild and pond carp, cyprinus carpio l., has been studied by light, fluorescence and electron microscopy. "gomori-positive" neurosecretory fibres are abundant in the main trunk of the neurohypophysis as well as its roots penetrating the pars intermedia. terminals of these fibres are in contact with capillaries of the general circulation and with glandular cells of the pars intermedia. monoaminergic fibres with a weak green fluores ...	1984	6478476
comparison of muscle proteinase activity among fish species.	the effects of temperature during storage, portion of muscle and growth stage of fish on the activity level of carp muscle acid (cathepsin d, ec 3.4.23.5), neutral and alkaline proteinases were examined. icing storage, freezer storage and portion of muscle did not affect each proteinase activity (p less than 0.05), but acid proteinase activity was affected by growth stage (p less than 0.05) and the level decreased from small to large fish. the activity levels of three kinds of proteinases were c ...	1984	6391798
[non-phosphorylative transglycosylation in extracts from white and red muscles of carp (cyprinus carpio l.)].	the enzymatic, nonphosphorylative reaction of transglycosylation in extracts from white and red muscles of the carp was studied. amylomaltase (ec 2.4.1.25) activity was assayed using p-nitrophenyl-n-maltoside as substrate and the amount of p-nitrophenyl-n-maltotrioside was assumed as a measure of the enzyme activity. the ph and temperature optimum of the reaction was examined. in both types of muscles significant differences in transglycosylative activity and its decrease immediately after prolo ...	1984	6242767
effects of a herbicide on the peroxide metabolism enzymes and lipid peroxidation in carp fish (hypophthalmichthys molitrix).	a study was made of the peroxide metabolism enzymes in carp fish, and of the effects on these of three different concentrations of the herbicide paraquat. mainly the changes in the superoxide dismutase, catalase and lipid peroxidation resembled the changes observed in previous intoxication studies on mammals.	1984	6239479
the mixed leukocyte reaction (mlr) in carp: bidirectional and undirectional mlr responses.	two-way and one-way mixed leukocyte reaction (mlr) was demonstrated in peripheral blood leukocytes (pbl) of carp. primary two-way mlr in randomly selected donor pairs were highly variable. weak primary responses could be strongly augmented by mutual in vivo priming of the reacting donors. one-way mlr was performed using irradiated (16,000 r) allogeneic pbl as stimulators. reciprocal responses of randomly paired donors were usually unequal, suggesting the usefulness of this method for genetic ana ...	1984	6238857
monoclonal antibodies against spermatozoa of the common carp (cyprinus carpio l.). i. a study germ cell antigens in adult males and females.	eleven monoclonal antibodies that recognize membrane determinants on spermatozoa of the carp cyprinus carpio l. have been produced. indirect immunofluorescence revealed that these determinants are uniformly distributed on the surface of head and midpiece. most of them are also present on the outer membrane of precursor sperm cells. although none of the monoclonal antibodies reacted with carp somatic tissue, five monoclonal antibodies were positive for surface membrane determinants of oogonia and ...	1984	6201282
a comparison of physiological changes in carp, cyprinus carpio, induced by several pollutants at sublethal concentration--ii. the dependency on the temperature.	carp were exposed to 10 different pollutants with sublethal concentrations at 12, 17 or 22 degrees c. the effects on the serum cortisol and glucose levels, the amount of liver and muscle glycogen and the concentration of protein and cholesterol in the serum were examined. the level of serum cortisol and glucose increased, the amount of liver and muscle glycogen decreased and the protein and cholesterol concentration were reduced after exposure to the pollutants. the cortisol and glucose response ...	1984	6149876
a technique for the determination of the available oxygen in living carp (cyprinus carpio) muscle.	a polarographic method for the measurement of the available oxygen in the muscle of living carp by the use of a platinum microelectrode is proposed. the oxygen and the reference electrodes were assembled in a single insertion piece which was implanted in the muscle of a living carp maintained in a special experimental chamber. curves for normal oxygen levels corresponding to air-saturated water, as well as to a carbogene-saturated water, were obtained. the method can be considered adequate for t ...	1984	6149042
some properties of monoamine oxidase in carp heart.	studies using clorgyline, deprenyl and semicarbazide as inhibitors showed that carp heart homogenate contained a new type of monoamine oxidase (mao) and a clorgyline- and deprenyl-resistant amine oxidase (crao). the deamination of monoamines by carp heart mao proceeded in two steps by a double-displacement (ping-pong) mechanism. the km values of the mao for oxygen (k0 values) with tyramine, 5-hydroxytryptamine (5-ht) and beta-phenylethylamine (pea) as substrates were identical (59 microm).	1984	6146456
studies on the properties of glucose-6-phosphatase from carp liver microsomes (cyprinus carpio l.).	the effects of temperature and ph on the phosphohydrolase activity of carp hepatic glucose-6-phosphatase (ec 3.1.3.9) have been investigated. the enzyme activity was maximum at about 308 k and in the ph range 5-6.5. the apparent michaelis constant (km) and vmax of the reaction with glucose-6-phosphate were found to be 14.8 mm and 2.27 nmol/min/mg protein. the enzyme activity was partly inhibited by edta, while in the presence of sufficient pcmb virtually total inhibition was observed.	1984	6086228
cellular types in the gill arch of the teleost cyprinus carpio (cyprinidae, pisces). a histochemical and ultrastructural study.	on adult specimens of the common carp (cyprinus carpio), we have carried out a histochemical and ultrastructural study of the epithelia which form the gill arch. secondary lamellae have two cellular types, granular and mucous, which produce neutral carbohydrates and proteins rich in tryptophane, and another mucous cell type which contains glycosaminoglycans. in gill rakers, three cellular types show different histochemical behaviour: 1) granular cells elaborate neutral mucosubstances; 2) a secon ...	1984	6083775
nuclear transplantation in teleosts. hybrid fish from the nucleus of crucian and the cytoplasm of carp.	hybrid fish are obtained from the combination of nucleus and cytoplasm from two genera of fresh-water teleosts using the technique of nuclear transplantation (i.e. the combination of the nucleus of crucian (carassius auratus) and the cytoplasm of carp (cyprinus carpio]. morphological characteristics of these hybrid fish that have been examined so far indicate that some features such as barbs, pharyngeal teeth, the number of scales along the lateral line, and the number of vertebrae are similar t ...	1984	6597566
an extensive projection of fish dorsolateral tegmental cells to the optic tectum revealed by intra-axonal dye marking.	the cells of the dorsolateral tegmental (dlt) nucleus in the crucian carp (carassius carassius) were physiologically identified and marked with lucifer dye. all the identified dlt cells receive both visual and rhombencephalic inputs. these cells project their axons into the contralateral tectum via the tectal commissure as well as into the ipsilateral tectum. the most striking characteristic of the dlt cells was the wide distribution of their axonal branching in the ipsilateral tectum.	1984	6207471
an electron microscopic study of two types of muscle fibers in the pharyngeal pad of crussian carp, carassius carassius.	in the pharyngeal pad on the roof of the anterior pharynx of carassius carassius two types of thin striated muscle fibers (1.5-10 micron in diameter) were found. no pattern was discerned in the orientation of muscle fibers which form a loose tissue as a whole. one of them (z fiber) is characterized by position of triads at the level of z lines, and in the other type (a-i fiber) they were seen at the junction of the a and i bands. three types of intermyofibrous junction are noted between muscle f ...	1984	6476414
light-dependent change of cone-horizontal cell interactions in carp retina.	carp h2 horizontal cells were investigated at various stages of dark and light adaptation. their state of adaptation was verified by evaluating in computer reconstructed serial sections the number of small, fingerlike protrusions originating from h1 processes. these so-called spinules are absent in the dark-adapted state and are reformed during light adaptation. intracellular recordings showed that long-wave-induced depolarization thought to be mediated by sign-inverted feedback from h1 horizont ...	1984	6326947
pineal neural connections with the brain in two teleosts, the crucian carp and the european eel.	the neural connections between the pineal organ and the brain were investigated in two teleost species: the crucian carp and the european eel. horseradish peroxidase (hrp) was administered into the pineal parenchyma in vivo or in vitro. after 12-96 hours, the brains were fixed, and hrp was reacted in frozen sections with either diaminobenzidine (dab) or hanker-yate's reagent. the hrp had entered the pineal neurons, and had labeled their axonal processes (the pineal tract) in a golgi-like manner. ...	1984	6085876
uptake, distribution, and elimination of sodium linear alkylbenzene sulfonate and sodium alkyl sulfate in carp.	uptake, distribution, and elimination of labeled surfactants, 35s-labeled sodium laurylbenzenesulfonate (35s-c12-las) and 35s-labeled sodium lauryl sulfate (35s-c12-as), were investigated in carp exposed to a low concentration level of the respective solutions found in river water in japan by whole-body autoradiography and liquid scintillation counting. whole-body autoradiograms showed that the fish absorbed 35s-c12-las and 35s-c12-as from the solutions principally through gills and that the sur ...	1984	215398
[the wormicidal substance of fresh water fishes on clonorchis sinensis v. purification and chemical characterization of clonorchicidal substance from epidermal mucus of cyprinus carpio]	as a series of studies to clarify clonorchicidal substances in body surface mucus of some fresh-water fishes, the substance in the epidermal mucus of cyprinus carpio was isolated by silica gel column and thin layer chromatography and analyzed for its chemical nature. wormicidal trial was done in vitro, and the results obtained are summarized as follows: the mucus was extracted by ethyl ether and separated into 4 fractions by column chromatography using benzene as solvent. the second fraction wit ...	1984	12891042
cloning, physical mapping and genome organization of mitochondrial dna from cyprinus carpio oocytes.	the mitochondrial genome from cyprinus carpio oocytes is a 10.5 megadalton, circular dna molecule. the carp mitochondrial dna was cloned in pbr325. three recombinant plasmids accounted for the entire genome. mapping of this dna using 11 different restriction endonucleases is reported here. both the large and small rrna genes were then localized using southern blot analysis. the subunit i of the cytochrome oxidase, the cytochrome b, the trnaglu and the urf 4 genes were localized by nucleotide seq ...	1984	6090866
synthesis of 4-substituted 2h-naphth[1,2-b]-1,4-oxazines, a new class of dopamine agonists.	a series of tricyclic oxazines, namely, the 4-substituted 2h-naphth[1,2-b]-1,4-oxazines, have been synthesized and assayed for dopamine agonist activity. one of the members of this series, compound (+)vii-15, was found to be a remarkably potent agonist in vivo when tested in the standard 6-hydroxydopamine lesioned rat assay. the absolute configuration of the compound corresponds to that found in the active isomer of apomorphine. its activity at the alpha 2 receptor (vs. [3h]clonidine) is relativ ...	1984	6094811
some properties of brain opioid receptors in membrane bound and solubilized state.	in brain membrane preparations, 3h-naloxone binding to bullfrog brain gave the highest values versus other species such as carp, chicken, rat and mouse. scatchard analysis revealed that the bmax value of bullfrog brain membranes was three fold greater than that of rat brain membranes. opioid receptors were solubilized from bullfrog brain membranes using digitonin or 3-(cholamidopropyl)-dimethylammonio-1-propane sulfonate (chaps) as the detergent. the properties of solubilized opioid receptors we ...	1984	6099495
[comparative characteristics of the properties of erythrocyte na,k-atpase in man and the carp cyprinus carpio].	studies have been made on some of the properties of na,k-atpase of a nuclear erythrocytes of man and nuclear erythrocytes of the carp cyprinus carpio. human erythrocytes yielded the enzymic activity only after their treatment by a detergent twin-20; under optimal conditions, it amounted to 2.6 mcmole /ml of erythrocytes per 1 h. in carp erythrocytes, na,k-atpase activity could be detected without detergent treatment, being 10-fold higher under optimal conditions than that in human erythrocytes. ...	1984	6145272
radioimmunochemical studies on 7.8s and 5.7s duck immunoglobulins in comparison with fab and fc fragments of chicken igy.	in ducks two forms of low molecular weight ig's +), 7.8s and 5.7s, have been described. to compare their antigenicity with fab and fc fragments of chicken igy a double-antibody ria technique was used in which the binding of 125i-chicken igy or 125i-chicken igy (fc) to rabbit and carp anti-chicken igy (fab) and anti-chicken igy (fc) antibodies, respectively, was inhibited by duck 7.8s and 5.7s ig's. chicken igy and duck 7.8s ig ( igy -like protein) are highly cross-reactive with respect to their ...	1984	6202568
specific activity of carp antisera against beta 2-microglobulin (beta 2m) and evidence for a beta 2m homologue in carp (cyprinus carpio).	antisera against rabbit and human beta 2-microglobulin were produced in carp (cyprinus carpio). these antisera were found to be specific for beta 2m, and detected beta 2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. in addition, these antisera were also inhibited by an extract from oyster. the differences between the reactivity of these two antisera and the ability of the anti-rabbit beta 2m to distinguish between ...	1984	6209173
aporphine enantiomers. interactions with d-1 and d-2 dopamine receptors.	the r(-) and the s(+) enantiomers of apomorphine (apo) and n-n-propyl norapomorphine (npa) interact with both the d-1 and the d-2 dopamine receptors. r(-)-apo, as well as r(-)- and s(+)-npa, stimulates the d-1 dopamine receptor in carp retina; s(+)-apo blocks this dopamine receptor. similarly, r(-)-apo, as well as r(-)- and s(+)-npa, stimulates the d-2 dopamine receptor in the intermediate lobe of the rat pituitary gland; s(+)-apo blocks the intermediate lobe d-2 receptor. the interactions betwe ...	1984	6231468
[a further confirmation of the high idiotypic specificity of carp anti-human myeloma protein sera].	previous investigations about the specificity of carp anti-idiotypic sera could be confirmed by comparison with corresponding antisera of guinea pigs. 3 carp antihuman myeloma-protein sera rendered as idiotypic specific after an appropriate absorption were tested in the passive hemagglutination inhibition test. they showed no difference to the parallel tested guinea pig antisera. it can be assumed that between fish antibodies and antibodies of mammals no functional difference exists in respect o ...	1984	6240937
stimulation in vitro of estradiol secretion by the ovary of a cichlid fish, sarotherodon aureus.	the objective of the present study was to investigate the nature of the gonadotrophic stimulation of estradiol (e2) secretion by the ovary of a teleost fish in vitro. spontaneous output of e2 decreased to a low baseline after 3 hr of superfusion and increased to a maximal level 2-3 hr after the introduction of homologous pituitary extract (t-pe) or human chorionic gonadotrophin into the superfusion system. addition of the phosphodiesterase inhibitor, isobutyl methylxanthine (ibmx, 0.5 mm), into ...	1984	6321293
comparison of the primary sequence of spring viremia of carp virus m protein with that of vesicular stomatitis virus.	the nucleotide sequence of spring viremia of carp virus mrna coding for the matrix protein (m) has been determined from a complete dna copy cloned in the escherichia coli plasmid pbr322. the clone is 710 nucleotides long and, excluding the 3' homopolymeric end (representing the mrna polyadenylic acid sequence), encodes a protein of some 223 amino acids (25,600 da). the protein has more basic than acidic amino acids and no significant amino- or carboxy-terminal hydrophobic domains. the predicted ...	1984	6324471
synthetic competitive antagonists of corticotropin-releasing factor: effect on acth secretion in the rat.	polypeptide analogs of the known members of the corticotropin-releasing factor (crf) family were synthesized and tested in vitro and in vivo for enhanced potency or competitive antagonism. predictive methods and physicochemical measurements had suggested an internal secondary alpha-helical conformation spanning about 25 residues for at least three members of the crf family. maximization of alpha-helix-forming potential by amino acid substitutions from the native known sequences (rat/human and ov ...	1984	6326264
[test systems for biomonitoring based on membrane-bound enzyme complexes. iv. assessment of genotoxic effects in an ames test system with metabolic activation by the microsomal mono-oxygenases from fish livers].	the study of mutagenic effect of 2-aminoantracene and benz(alpha)pyrene on salmonella triphimurium ta 100 in the ames test-system in the presence of postmitochondrial fractions s-9 from carp liver with 3-methylcholantrene induced by microsomal oxidation system has been carried out. the metabolic activity and cytochrome p450 contence in carp liver microsomes have been shown to concede considerably those in rats liver. but these characteristics are sufficient for the use of fraction s-9 from carp ...	1984	6329328
a high-resolution proton nuclear-magnetic-resonance investigation of carp hemoglobin. conformational differences between carp and human normal adult hemoglobins in solution.	the high-resolution proton nuclear magnetic resonance spectra of carp hemoglobin have been compared to those of human normal adult hemoglobin. carp deoxy and carbonmonoxy hemoglobins in the deoxy-type quaternary state exhibit two downfield exchangeable proton resonances as compared to four seen in human normal adult deoxyhemoglobin. this suggests that two of the hydrogen bonds present in human normal adult deoxyhemoglobin are absent or occur in very different environments in carp hemoglobin. one ...	1984	6329750
intracellular distribution of fumarase in various animals.	the subcellular distribution of fumarase was investigated in the liver of various animals and in several tissues of the rat. in the rat liver, fumarase was predominantly located in the cytosolic and mitochondrial fractions, but not in the peroxisomal fraction. the amount of fumarase associated with the microsomes was less than 5% of the total enzyme activity. the investigation of the intracellular distribution of hepatic fumarase of the rat, mouse, rabbit, dog, chicken, snake, frog, and carp rev ...	1984	6333419
carp preproinsulin cdna sequence and evolution of insulin genes.	the nucleic acid sequence of the preproinsulin cdna of carp (cyprinus carpio), cloned in the psti site of pbr322 ( liebscher et al. 1980), has been determined. the sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. the nucleotide sequence confirms the previously established amino acid sequence of carp insulin ( makower et al. 1982) and determines those of the s ...	1984	6373397
clonorchis sinensis: life cycle, intermediate hosts, transmission to man and geographical distribution in korea.	the snail host of clonorchis sinensis in korea is referred to as parafossarulus manchouricus, but it has been recorded also as bulimus striatulus japonicus in literature published up to late 1950. the specific name of the snail was corrected such that bulimus striatulus of china, japan, and korea should be incorporated in the species of p. manchouricus. in general, the snail exists in limited areas of the rivers han, kum, mankyung, naktong, and yeongsan, and the infestation of the snail with the ...	1984	6391501
[spore-forming bacteria in carp and rainbow trout, raised in waste waters from a steam power plant].		1984	6400797
pharmacologic profile of a novel potent direct-acting dopamine agonist, (+)-4-propyl-9-hydroxynaphthoxazine [(+)-phno].	the (+)-enantiomer of 1,2,3,4a,5,6-hexahydro-9-hydroxy-4-n-propyl-4h-naphth[1,2-b][ 1,4]-oxazine [(+)-phno] is demonstrated to be a potent and direct dopamine (da) agonist in several in vivo and in vitro test procedures. in vitro (+)-phno inhibited binding of [3h]apomorphine (ic50 = 23 nm) or [3h]spiperone (ic50 = 55 nm) to rat striatal membranes. because (+)-phno failed to stimulate adenylate cyclase in carp retina, it was classified as a d-2 agonist. ed50 values (shown in parentheses) derived ...	1984	6433000
[changes in the electrolyte composition of the blood and muscles in carp infection with ichthyophthirius multifiliis (ciliata, ophryoglenidae)].	a study of the electrolytic contents of blood serum, erythrocytes and muscles of carp infected with ichthyophthirius multifiliis was carried out. whatever the infection intensity, a characteristic increase in the cations k+ and na+ ratio in the plasma and muscles of infected fishes takes place. the above dependence, which is expressed in the intensification of active ions transport, is associated with mobilization of organism's protective functions.	1984	6441141
stereochemistry of atp and gtp bound to fish haemoglobins. a transferred nuclear overhauser enhancement, 31p-nuclear magnetic resonance, oxygen equilibrium and molecular modelling study.	this study was undertaken in order to test the models of atp and gtp binding to carp deoxyhaemoglobin proposed by perutz & brunori (1982) and to find out why gtp is a more potent allosteric effector than atp. we have determined the conformations of both nucleoside triphosphates by nuclear magnetic resonance studies and found them to be the same. the purines are in anti conformation about the glycosidic bond that links them to the ribose; the pentose ring is 3'-endo; the p-o5'-c5'-c4' torsion ang ...	1984	6492161
proton nuclear magnetic resonance investigation of the allosteric transition in ligated and unligated carp hemoglobin. evidence for structural heterogeneity in the heme pocket.	the proton nuclear magnetic resonance spectra of carp hemoglobin (hb) in the unligated deoxy and ligated met-cyano and met-azido forms have been recorded as a function of ph and upon addition of inositol hexaphosphate. all protein derivatives yield spectra that are consistent with appreciable molecular heterogeneity in the heme cavity. the pattern of heme methyl hyperfine shifts in carp met-cyano hb indicates that this heterogeneity arises from the presence of heme rotational disorder, as found ...	1984	6492169
a field-potential study of centripetal and centrifugal connections of the olfactory bulb in the carp, cyprinus carpio (l).	the projection areas of mitral cell axons in the lateral (lot) and medial (mot) olfactory tracts and the pathways of two groups of centrifugal fibers (with fast and slow conduction velocities) to the olfactory bulb were studied in the carp using the field potential method. mitral cell axons of the lot and those of the mot projected to the overlapping areas in the bilateral telencephalon and preoptic area (poa), the contralateral mot, and the nucleus posterior tuberis (npt) in the diencephalon. h ...	1984	6498513
new anomalies found in the 11q-syndrome.	the cytogenetic analysis of an infant with multiple congenital anomalies revealed a small deletion of the long arm of one no. 11 chromosome: 46xx del 11 (q23-q25). the main clinical manifestations included: trigonocephaly, flat broad nasal bridge, micrognathia, carp mouth, hypertelorism, low set ears, severe congenital heart disease, anomalies of limbs and external genitalia. in comparison to the previously reported cases of 11q-, the patient presented here had congenital anomalies not described ...	1984	6499268
structural and functional analysis of spontaneous anti-nitrophenyl antibodies in three cyprinid fish species: carp (cyrinus carpio), goldfish (carassius auratus) and tench (tinca tinca).	high spontaneous anti-trinitrophenyl (tnp) activities were found in three cyprinid fish species: carp (cyprinus carpio), goldfish (carassius auratus) and tench (tinca tinca). the molecules involved, isolated by affinity chromatography on dinitrophenyl-lysine sepharose (dnp-lysine-sepharose), had the main characteristics of a high molecular weight immunoglobulin (igm-like). affinity measurements were performed on natural anti-dnp/tnp antibodies isolated from nine individual tench sera, using the ...	1984	6500139
[polymorphism of trypanosoma gruby, 1841 found in the blood of carp (carassius auratus gibelio (bloch), perch (perca fluviatilis l.) and stone perch (acerina cernua (l.))].		1984	6516399
[acid phosphatase activity in the internal organs of carp (cyprinus carpio l.) infected with tapeworm khawia sinensis (hsü, 1935) and bothriocephalus gowkongensis (yeh, 1955)].		1984	6516402
[transport of benzo(a)pyrene into animal organs following the feeding of activated sludge from swine manure].	in feeding experiments with pigs and fish (carp and trout) the test animals received quotas of activated sludge of 7.5 and 15% dm (pigs) and 20% (carp) in their mixed feed. in an experiment with trout in an aquarium the quota of activated sludge was increased from 20% to 50%. the benzo(a)pyrene content was ascertained in selected organs of the pigs (kidney, liver, bacon, muscles) and the edible parts of the fish. as a result of this experiment one can say that the feeding of activated sludge fro ...	1984	6517694
magnetic susceptibility of oxy- and carbonmonoxyhemoglobins.	the room temperature magnetic susceptibilities of human and carp oxy- and carbonmonoxyhemoglobin solutions were measured in a 30-gauss (1 g = 10(-4) t) field with a superconducting magnetometer. to within experimental uncertainty, the susceptibility was the same for both the oxy and carbonmonoxy forms, and salt concentration did not effect it. a variety of sample preparations were used; the iron chemical state was verified by mössbauer spectroscopy. a value of -0.580 +/- 0.010 x 10(-6) centimete ...	1984	6591198
evolution of low molecular weight immunoglobulins--iv. igy-like immunoglobulins of birds, reptiles and amphibians, precursors of mammalian iga.	radioimmunochemical studies on the comparison of the immunological cross-reactivity between the 7-s igs of birds, reptiles and amphibians (igy-like igs) and the iga of mammals (man and pig) using 125i-chicken igy and anti-chicken igy(fc) or anti-chicken igy(h) antibodies from rabbits and carp for the detection led to the conclusion that there are close antigenic relationships between them. therefore, the igy-like igs seem to be the precursors for the iga class of mammals. from that, we give a ph ...	1984	6611490
detection of calcium binding proteins by 45ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis.	a new simple method of detecting calcium binding proteins in a protein mixture is described. a sample which might include calcium binding proteins was subjected to sds-polyacrylamide gel electrophoresis and then electrophoretically transferred to a nitrocellulose membrane. the membrane was then incubated with 45ca to detect calcium binding proteins as radioactive bands by autoradiography. purified troponin-c, calmodulin, myosin dtnb light chain, and parvalbumin were clearly identified by this me ...	1984	6715311
regulatory effect of dopamine on spatial properties of horizontal cells in carp retina.	three types of light-induced response (l-, rg-, and yrb -type s-potentials) recorded from isolated retinas of the carp (cyprinus carpio) were identified by their spectral response and later by morphological localization of the recording sites marked with an intracellular lucifer yellow (ly). horizontal cells in a given layer, generating one of the above response types, are electrically coupled via gap junctions, so that the injected ly normally diffused to several neighboring cells. the spatial ...	1984	6726332
collagen hydrolysis by a new streptomyces species.	a soil streptomycete hydrolysed collagen extracted from bovine achilles tendon, calf skin, carp swim bladder and rat tail tendon. enzyme activity was highest with rat tail tendon collagen as substrate. the taxonomy of the streptomycete was studied according to internationally approved methods. comparative studies revealed that it resembled streptomyces humidus , s. rochei and s. distatochromogenes in some respects, but differed in many cultural and physiological characteristics. based on these d ...	1984	6727696
oxygen equilibrium studies on carp-human hybrid hemoglobins.	hybrid hemoglobins have been made in which one chain is derived from human hemoglobin and the other from carp hemoglobin. both hybrid hemoglobins show low cooperativity in oxygen binding. hybrid i (alpha carp: beta human) has a very small bohr effect, whereas hybrid ii (alpha human: beta carp) has a bohr effect nearly as large as that for human hemoglobin. both hemoglobins have p50's more closely resembling carp hemoglobin than human hemoglobin in the region of ph 7, and for both hybrids, as for ...	1984	6733079
ligand binding kinetic studies on the hybrid hemoglobin alpha(carp):beta(human): a hemoglobin with a restricted allosteric range.	the preparation of native alpha- and beta-chains of carp hemoglobin and the preparation of the hybrid hemoglobin alpha(carp):beta(human), hybrid i, are described. co association and dissociation kinetics were determined for the hybrid hemoglobin alpha(carp):beta(human) as a function of fractional saturation by stopped-flow and by flash and laser photolysis. oxygen dissociation from the liganded distribution of conformers and overall oxygen association were studied by laser photolysis. an alloste ...	1984	6733080
ligand binding kinetic studies on the hybrid hemoglobin alpha (human):beta (carp): a hemoglobin with mixed conformations and sequential conformational changes.	oxygen and co ligand binding kinetics have been studied for the hybrid hemoglobin (hb) alpha (human):beta (carp), hybrid ii. valency and half-saturated hybrids were used to aid in the assignment of the conformations of both chains. in hybrid ii, an intermediate s state occurs, in which one chain has r- and the other t-state properties. in hbco at ph 6 (plus 1 mm inositol hexaphosphate), the human alpha-chain is r state and the carp beta-chain is t state. we have no evidence at this ph that the c ...	1984	6733081
primary structures of multiple forms of urotensin ii in the urophysis of the carp, cyprinus carpio.	multiple forms of urotensin ii (uii), one of the hormonal peptides of the caudal neurosecretory system of fishes, were purified from the urophyses of the carp, cyprinus carpio. three distinct peaks with uii activity (classified as uii-alpha, -beta and -gamma) were separated by reverse-phase high-pressure liquid chromatography (hplc). edman degradation as well as digestion with carboxypeptidase a revealed the primary structures of these peptides as uii-alpha: gly-gly-gly-ala-asp-cys-phe-trp-lys-t ...	1984	6745627
connectin content and its post-mortem changes in fish muscle.	connectin was isolated from fish dorsal myofibrils by an sds-gel filtration method and estimated to account for approximately 13% of the total myofibrillar proteins. there was no significant difference in the amount of connectin among seven fish species but rabbit skeletal myofibrils contained a slightly higher content (16%) of connectin. the high molecular weight connectins from carp and rabbit both showed a doublet band, consisting of bands 1 and 2, on sds-polyacrylamide gel electrophoresis us ...	1984	6746595
enzymic and molecular characteristics of a new form of monoamine oxidase, distinct from form-a and form-b.	the present study was undertaken to clarify the enzymic and molecular properties of monoamine oxidase (mao) in carp brain. in particular, its sensitivities to selective mao inhibitors, kinetic properties and molecular weight were compared with those of the enzyme in carp liver. the selective and potent mao-a and mao-b inhibitors fla 788(+), fla 336(+), md 780236 and benzylcyanide caused dose-dependent inhibitions of mao activity in both carp brain and liver; the inhibition curves were all single ...	1984	6748374
[peculiarities of the amino acid composition, spatial organization and interaction with dna of histones h1 from calf thymus and carp spermatozoa].	the amino acid composition of the h1-like histone isolated from carp spermatozoa (h1carp) is characterized by a high content of lysine (34.6%) and a low content of glycine (4.5%) as compared to that of its calf counterpart (h1calf). the lys/arg ratio is 21.6, which is much higher than that for the h1-like histones from other species spermatozoa (cf. echinodermata). it was shown that the fluorescence anisotropy and excitation spectra of histones h1carp and h1calf change synchronically. at the sam ...	1983	6615921
lateral spread of light-induced response at the cell body and axon terminal levels of external horizontal cells in the carp retina.	one type of light-induced response (photopic l-type s-potential)recorded from isolated carp (cyprinus carpio) retinas was identified by its spectral response and later confirmed by morphological localization of the recorded sites ionophoretically marked by a fluorescent dye, lucifer yellow. such l-type s-potentials could be recorded from the soma and from the axon terminal of external horizontal cells. the spatial property of the s-potentials from the soma was compared with that recorded from th ...	1983	6632374
ligand binding to heme proteins. an evaluation of distal effects.	the o2, co, and alkyl isocyanide-binding properties of a variety of vertebrate and invertebrate heme proteins have been compared in detail to those of protoheme mono-3-(1-imidazoyl)-propylamide monomethyl ester in aqueous suspensions of soap micelles. the proteins examined include: cytochrome p-450cam from pseudomonas putida, beef heart cytochrome c oxidase, yeast cytochrome c peroxidase, alpha and beta subunits of human hemoglobin, sheep hemoglobin, carp hemoglobin, sperm whale myoglobin, horse ...	1983	6643477
evidence for the homology of hexose 6-phosphate dehydrogenase and glucose 6-phosphate dehydrogenase: comparison of the amino acid compositions.	amino acid compositions of hexose 6-phosphate dehydrogenase and glucose 6-phosphate dehydrogenase from crucian carp and rat, and that of sea-urchin glucose 6-phosphate dehydrogenase were compared in order to ascertain if they are homologous enzymes. the results demonstrated that the overall amino acid compositions of the two enzymes remarkably resemble each other, and that the composition divergence (d = 0.038) of the crucian carp enzymes is in the range of those for homologous proteins so far r ...	1983	6661901
scanning electron microscopic study of the intestinal mucosa of an indian freshwater adult major carp, labeo rohita (hamilton).	fine anatomical structures of the different regions (viz., anterior, middle and posterior) of the intestinal mucosa in adult labeo rohita (hamilton) have been studied by using scanning electron microscope (sem). surface mucosal modifications of the anterior and the middle of the intestine not only increase the luminal surface by 2-4 times but serve also the storage function of the ingested food due to the absence of stomach in the fish studied. fine anatomical structures of the columnar epitheli ...	1983	6670351
preliminary study on the effect of temperature on the transport of thyroxine (t4) into the body tissues and sub-cellular fractions of liver and muscle of carp, cyprinus carpio.	in order to determine if the inability of thyroxine to induce cellular effects at low temperature is mediated through a temperature-sensitive system for the translocation of t4 into the nucleus, the effect of temperature on the uptake of t4 by body tissues and sub-cellular fractions of carp liver and muscle was studied in vivo. a single injection of 125i-t4 (1 micro c/10 g body weight) was given intraperitoneally to juvenile carp maintained at 15 and 25 c. uptake from the peritoneal cavity was r ...	1983	6670568
[establishment of a fish-virus susceptible cell strain from the pituitary gland of carp (cyprinus carpio l.)].	a new continuous cell strain (capi) was established from normal pituitary tissue of carp, cyprinus carpio l. cells of a fractionated dispersion (0.25% trypsin-pbs, 10 degrees c) of pituitaries formed monolayer (fibroblast- and epithelial-like-cells) at 25 degrees c using eagle's minimal essential medium (mem) supplemented with 10% fetal calf serum and non essential amino acids. the continuous cell strain, designated capi, was obtained by an enzymatical selection of epitheloid cells from the mono ...	1983	6675352
aporphines. 48. enantioselectivity of (r)-(-)- and (s)-(+)-n-n-propylnorapomorphine on dopamine receptors.	the enantiomers [(s)-(+) and (r)-(-)] of n-n-propylnorapomorphine (npa) were synthesized. (r)-npa was obtained by the acid-catalyzed rearrangement of n-n-propylnormorphine. (r)-npa also was converted to (rs)-n-n-propylnorapomorphine dimethyl ether by dehydrogenation of the 10,11-o,o'-dimethyl ether of (r)-npa with 10% palladium on carbon in acetonitrile, followed by reduction with sodium cyanoborohydride under acidic conditions. alternatively (rs)-npa 10,11-o,o'-dimethyl ether was obtained via t ...	1983	6682147
a new type of mitochondrial monoamine oxidase distinct from type-a and type-b.	the characteristics of mitochondrial monoamine oxidase (mao) in carp liver were studied with mao inhibitors and substrates. this enzyme was thermolabile, but was stabilized in the presence of bovine serum albumin. with clorgyline and deprenyl, single-sigmoidal curves for inhibition of the activity towards tyramine or 5-hydroxytryptamine were obtained; the sensitivities to the two inhibitors were identical. the activity towards beta-phenylethylamine was not completely inhibited by clorgyline or d ...	1983	6823208
primary structure of hemoglobin from trout (salmo irideus) amino acid sequence of the beta chain of trout hb i.	the amino acid sequence of the beta chain of trout hb i is presented; it adds to the previously reported sequence of the alpha chain (bossa et al. (1978) biochim. biophys. acta 536, 298-305), thus completing the primary structure of the hemoglobin component of trout's blood devoid of heterotropic phenomena. comparison of beta chain from trout hb i with the corresponding sequences from human and carp shows differences of 46.6% and 34.7%, respectively; the sequence (almost completed) of the beta c ...	1983	6824687
vertebrate skin type i collagen: comparison of bony fishes with lamprey and calf.	1. characterization of type i collagen alpha chains, alpha 1 and alpha 2, in the skin tissues of carp and common mackerel revealed a marked interspecies difference in cnbr-peptide maps of the alpha 2 chains, suggesting the hypervariability during evolution of the alpha 2 chains relative to the alpha 1 chains. 2. when compared with calf type i collagen, lower vertebrate type i collagens derived from these bony fishes as well as from lamprey were found to exhibit a higher degree of structural simi ...	1983	6839718
magnetic and spectral properties of carp carbonmonoxyhemoglobin. competitive effects of chloride ions and inositol hexakisphosphate.	we have extended our studies on the magnetic properties of carp carbonmonoxyhemoglobin and the dependence of these properties upon solution variables. using an improved version of the superconducting magnetometer, we have found that the magnetic susceptibility of carp carbonmonoxyhemoglobin is sensitive to both inositol hexakisphosphate and chloride ion. the dependence upon chloride ion concentration is complex. at relatively low concentrations this anion reverses the effect of inositol hexakisp ...	1983	6852007
quaternary structure and spin equilibria in ferric hemoglobins. a room temperature study.	the effective magnetic moments for a number of human and carp methemoglobin derivatives were determined in solution at room temperature. the data permit us to confirm the dependence of the spin-state equilibrium of azide methemoglobin on the quarternary state of the hemoglobin and to demonstrate a similar dependence for both human and carp aquomethemoglobin. in addition, the ph dependence of the effective magnetic moment and the soret spectrum of carp azidemethemoglobin are compared.	1983	6852055
ionic mechanisms underlying the responses of off-center bipolar cells in the carp retina. i. studies on responses evoked by light.	off-center bipolar cells show hyperpolarizing responses to spot illumination in the receptive field center and depolarization responses to an annulus in the surround. to understand the ionic mechanisms underlying these responses, we examined the current-voltage relationship of these bipolar cells, input resistance changes during their light-evoked responses, and the reversal potentials of these responses. off-center bipolar cells generally showed inward rectification when they were hyperpolarize ...	1983	6854267
ionic mechanisms underlying the responses of off-center bipolar cells in the carp retina. ii. studies on responses evoked by transretinal current stimulation.	transretinal current pulses flowing from the receptor side to the vitreous side of the retina cause transient release of transmitter from the photoreceptor terminals, and in off-center bipolar cells they evoke transient depolarizations with a brief (less than 1 ms) synaptic delay. since it is known that the presence of na+ in the external medium is not essential for this type of transmitter release, we used this procedure to examine the role of [na+]o in the generation of light-evoked responses ...	1983	6854268
[studies on metabolism of mercury compounds in mouse, rat and carp--with special reference to biological half lives and distribution of mercury compounds in whole body and in individual organs].		1983	6865087
[effect of calcium binding on the quenching time of self-fluorescence of ca-binding proteins].	lifetimes of phenylalanine, tyrosine and tryptophan self-fluorescence of three ca2+-binding proteins (parvalbumins pi 4.47 and 3.95 and bovine alpha-lactalbumin) in the ca2+-saturated state and without ca2+ were measured on a device functioning in a channel of synchrotron radiation of the lebedev physical institute electron accelerator c-60 with a single photon counting system. the decay curve of phenylalanine fluorescence of ca2+-saturated parvalbumin pi 4.47 is two-exponential, which results f ...	1983	6871261
evolution of low molecular weight immunoglobulins--iii. the immunoglobulin of chicken bile--not an iga.	studies on comparison of antigenic relationships between the immunoglobulin of chicken bile--the so-called chicken iga--and human, porcine, as well as murine iga were performed by a series of double-antibody and solid-phase ria systems using h chain-specific anti-chicken biliary ig sera and alpha chain-specific anti-human iga sera produced in rabbits and in carp. it was not possible to demonstrate a significant immunological cross-reactivity of the ig of chicken bile to the ig's of iga class. be ...	1983	6877244
[morphohistochemical studies of the carp intestines in bothriocephaliasis].	morphohistochemical study of host-parasite relationships after the infection of carp with bothriocephalus opsariichthydis was conducted. it has been found out that the whole parasite's strobile comes into close contact with tissues of the mucous membrane of the carp's gut. the destruction of the gut's wall results from its digestion or lysis that is confirmed by high activity of acid phosphatase. together with epithelium of the mucous membrane of the gut exodermis of the helminth takes an active ...	1983	6877860
double color-opponent receptive fields of carp bipolar cells.	bipolar cells with double color-opponent receptive fields were found in the carp retina. they were classified into two types depending on their response polarity. in the receptive field center, type i cells showed hyperpolarization to a long wavelength spot (r-) and depolarization to a short wavelength spot (g+), while in the surround they showed r+, g- responses. type ii cells showed responses of opposite polarities to those of type i cells, namely r + g- in the center, and r - g+ in the surrou ...	1983	6880036
5-hydroxytryptamine: its facilitative action on [3h]dopamine release from the retina.	5-hydroxytryptamine (5-ht) at 5 x 10(-4) m caused a two-fold increase in [3h]dopamine (da) release from retinal particulate fractions of the carp (cyprinus carpio). the 5-ht action was dose- and ca2+-dependent. the three 5-ht agonists examined (5-methoxytryptamine, 5-methoxy-n,n-dimethyltryptamine and tryptamine) were more effective than 5-ht on [3h]da release. 5,6-dihydroxytryptamine was the strongest competitor among drugs tested for [3h ]da uptake, but did not evoke any significant release of ...	1983	6880041
characterization of the active site, substrate specificity and kinetic properties of acetyl-coa:arylamine n-acetyltransferase from pigeon liver.	it could be demonstrated that a sulfhydryl group is involved in the catalysis of acetyl-coa:arylamine n-acetyltransferase from pigeon liver (ec 2.3.1.5). from ping-pong kinetics it was concluded that there is a covalent acetyl-enzyme intermediate. the respective intermediate could be isolated and chemically characterized as a cysteinyl thioester. electrophoretically homogeneous acetyl-coa:acylamine n-acetyltransferase from pigeon liver was able to acetylate a broad variety of aromatic and alipha ...	1983	6882770
[distal 14q trisomy].	a 22-month-old boy was found to be trisomic for distal 14q due to malsegregation of a t(10;14)(q26.3;q32.1)pat. this observation and seven others reported in the literature permit the delineation of the corresponding clinical syndrome, which includes the following : intra-uterine and postnatal growth retardation; craniofacial dysmorphism : a large facies, chubby cheeks, facial asymmetry, hypertelorism, a broad nose, a short prominent philtrum, a carp-shaped mouth, micrognathia, low-set ears with ...	1983	6606379
[cross-reactivity of 3m kcl-extracts of fetal tissue from various species in the macrophage electrophoresis mobility test. ii. tissue extracts from lower vertebrates in various phases of development].	cellular sensitization was detected by the mem-test using 3m kcl-extracts of fetuses from man and mouse and of different metamorphic stages of the water frog (tadpoles) and the carp (yolk sack-bearing stage). the sensitization demonstrated was independent on the type of tumor tested. the occurrence of active components in the extracts was correlated with certain stages of individual development of water frog and carp. the evidence of these components in species of several classes of vertebrates ...	1983	6606408
carp preproinsulin cdna sequence and evolution of insulin genes.	the nucleic acid sequence of the preproinsulin cdna of carp (cyprinus carpio), cloned in the psti-site of pbr322 (1), has been determined. the sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. the nucleotide sequence confirms the previously established amino acid sequence of carp insulin (2) and determines those of the signal (21 aa 1) and c-peptide (35 aa 1). ...	1983	6306593
isolation, analysis of structure, synthesis, and biological actions of urotensin i neuropeptides.	the 41-residue neuropeptide urotensin i (ui), from the urophyses of two teleost fish species (cyprinus carpio and catostomus commersoni), was isolated and purified, and its amino acid sequence was determined and confirmed by synthesis of a fully active peptide. the ui peptide was found to be a close structural and biological homologue of the ovine hypothalamic corticotropin-releasing factor (crf) and the frog skin peptide sauvagine; ui is, therefore, a phylogenetic prototype of this group of pep ...	1983	6313156
poly(adp-ribose) synthetase associated to cytoplasmic structures of meiotic cells.	an active poly(adp-ribose) synthetase has been found to be associated to mitochondria of frog oocytes and of testis from various species. the enzyme was also found in association with the microsomal-ribosomes fraction of the testis. both enzymes are strongly inhibited by nicotinamide, thymidine, and theophylline, and they synthesize oligomers of adp-ribose of an average chain length of 2-6 residues. the apparent km for nad+ of the mitochondrial enzyme is 22 micro m which contrasts with the corre ...	1983	6317634
rds-127 (2-di-n-propylamino-4,7-dimethoxyindane): central effects of a new dopamine receptor agonist.	apomorphine (apo), 2-di-n-propylamino-4,7-dimethoxyindane (rds-127) and 2-di-n-propylamino-5,8-dimethoxytetralin (jmc-181) were examined on a variety of biochemical and pharmacological assays to determine their possible interaction with dopamine (da) receptors. nanomolar concentrations of all three compounds displaced [3h]apo from specific high-affinity binding sites in rat striatal membrane preparations, while higher concentrations were required to displace [3h]spiperone or [3h]rauwolscine. apo ...	1983	6401334
comparative study of the glial fibrillary acidic protein in vertebrates by pap immunohistochemistry.	glial fibrillary acidic protein (gfa) has been visualized by direct peroxidase antiperoxidase (apa) immunohistochemistry in various vertebrates (cyclostomes, teleosts, amphibians, reptiles, birds, and several placental mammals). in this study gfa-immunoreactivity (gfa-i) was observed in all species examined except in cyclostomes and amphibians. two types of immunoreactive elements were observed: astrocytes and long processes without visible somata. astrocytic cells with gfa-i were first found in ...	1983	6408144
[necrotic processes in the gill tissue of carp caused by changes in the aquatic environment].	histological findings show that various pathogenic processes are involved in the cases of clinically detected branchionecrosis. the changes in gills occurring at toxic branchionecrosis are a necrobiotic process in their nature; in gill lamellae this process manifests itself as a swelling of the respiratory epithelium and as dilation of lamellar sinuses, and in stratified epithelium of gill arch and filaments it takes on the form of the hypertrophy and hyperplasia of epithelial cells. in severe c ...	1983	6419439
separation of lymphocyte subpopulations in carp cyprinus carpio l. by monoclonal antibodies: immunohistochemical studies.	lymphoid cell populations in various organs of the carp cyprinus carpio l. were investigated using a series of mouse monoclonal antibodies raised against carp thymocytes and carp serum ig. clones have been designated as ig+t+, ig+t- or ig-t+ on the basis of the reactivity with thymocytes and serum ig in the enzyme-linked immunosorbent assay (elisa) screening. their reaction to the lymphoid organs of carp was investigated on cryostat sections and cytocentrifuge slides using immunoperoxidase techn ...	1983	6336715
identification of a heparin activated amidolytic enzyme in carp (cyprinus carpio) plasma.	a heparin activated amidolytic enzyme capable of cleaving synthetic thrombin sensitive chromogenic substrates was identified from the plasma of the carp (cyprinus carpio). activation was inhibited by kcl, protamine sulphate and human plasma. heparin was not required for the continued action of the enzyme. active enzyme was irreversibly inhibited by dfp. the ph and temperature optima was studied and the enzyme semi purified by sephadex g100 superfine and sephadex a50 chromatography. an approximat ...	1983	6342205
immunohistochemical demonstration of serotonin-containing csf-contacting neurons in the submammalian paraventricular organ.	the distribution and morphological aspects of the serotonin-containing neurons in the paraventricular organ of the carp, frog, turtle and chicken were studied by means of an immunoperoxidase technique using serotonin antiserum. in all species the serotonin-containing neurons were seen to have the appearance of the csf-contacting neurons and to be distributed in the pars ependymalis and the pars hypendymalis of the organ. particularly, in the frog, the serotonin-containing csf-contacting neurons, ...	1983	6345483