Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| salmonella enterica serotype typhimurium dt104 arta-dependent modification of pertussis toxin-sensitive g proteins in the presence of [32p]nad. | salmonella enterica serotype typhimurium (s. typhimurium) definitive phage type (dt) 104 has become a widespread cause of human and other animal infections worldwide. the severity of clinical illness in s. typhimurium dt104 outbreaks suggests that this strain possesses enhanced virulence. arta and artb - encoded by a prophage in s. typhimurium dt104 - are homologues of components of pertussis toxin (ptx), including its adp-ribosyltransferase subunit. here, we show that exposing dt104 to mitomyci ... | 2009 | 19696112 |
| development of an indirect elisa for the detection of serum igg antibodies against region iv of phase 1 flagellin of salmonella enterica serovar brandenburg in sheep. | region iv of phase 1 flagellin (flic) of salmonella enterica serovar brandenburg (s. brandenburg) was expressed in escherichia coli and purified by nickel chelate affinity chromatography. the purified recombinant protein was evaluated for its suitability as an antigen in an indirect elisa for the detection of antibodies in sheep sera. a cut-off value of 0.1 was calculated using 80 serum samples collected from sheep with no previous history of s. brandenburg. in the present study we show the resu ... | 2009 | 19696151 |
| plasmid-mediated 16s rrna methylases among extended-spectrum-beta-lactamase-producing salmonella enterica senftenberg isolates from algeria. | 2009 | 19700474 | |
| cloning of salmonella enterica serovar enteritidis fimbrial protein sefa as a surface protein in escherichia coli confers the ability to attach to eukaryotic cell lines. | the gene for the salmonella enterica serovar enteritidis fimbrial protein sefa was cloned into an escherichia coli surface expression vector and confirmed by western blot assay. e. coli clones expressing sefa attached to avian ovary granulosa cells and hep-2 cells, providing evidence for the involvement of sefa in the ability of salmonella to attach to eukaryotic cells. | 2009 | 19700549 |
| absence of functional hfe protects mice from invasive salmonella enterica serovar typhimurium infection via induction of lipocalin-2. | mutations of hfe are associated with hereditary hemochromatosis, but their influence on host susceptibility to infection is incompletely understood. we report that mice lacking one or both hfe alleles are protected from septicemia with salmonella typhimurium, displaying prolonged survival and improved control of bacterial replication. this increased resistance is paralleled by an enhanced production of the enterochelin-binding peptide lipocalin-2 (lcn2), which reduces the availability of iron fo ... | 2009 | 19700664 |
| introduction of new multidrug-resistant salmonella enterica strains into commercial dairy herds. | a longitudinal observational study of 59 dairy herds was conducted in washington state to estimate the rate of introduction of new multidrug-resistant (mdr) salmonella enterica strains onto commercial dairy herds. samples were collected on these herds over 7 visits separated by intervals of 2 to 4 mo over a period of 15 to 21 mo. samples were cultured for salmonella spp. and serogroup, serovar, and antimicrobial susceptibility patterns were identified for mdr salmonella isolates. fingerprinting ... | 2009 | 19700683 |
| survey of probiotic enterococci from traditional dairy products and their impact upon the infant intestinal microbiota. | 2009 | 19700927 | |
| quinolone-resistant salmonella enterica serovar typhi presenting as acute fulminant hepatitis. | 2009 | 19702041 | |
| the tvia auxiliary protein renders the salmonella enterica serotype typhi rcsb regulon responsive to changes in osmolarity. | in response to osmolarity, salmonella enterica serotype typhi (s. typhi) regulates genes required for vi capsular antigen expression oppositely to those required for motility and invasion. previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the rcsc/rcsd/rcsb phosphorelay system. here we performed gene expression profiling and functional studies to determine the role of tvia, an auxiliary protein of the rcsb response regulator, in control ... | 2009 | 19703107 |
| rcs signalling-activated transcription of rcsa induces strong anti-sense transcription of upstream flipqr flagellar genes from a weak intergenic promoter: regulatory roles for the anti-sense transcript in virulence and motility. | in salmonella enterica, an activated rcs signalling system inhibits initiation of transcription of the flhd master operon. under these conditions, where motility is shut down, microarray experiments showed an increased rna signal for three flagellar genes -flipqr- located upstream of rcsa. we show here that it is the anti-sense (as) strand of these genes that is transcribed, originating at a weak promoter in the intergenic region between flir and rcsa. rcsa is an auxiliary regulator for the rcs ... | 2009 | 19703110 |
| national surveillance of salmonella enterica in food-producing animals in japan. | a total of 518 fecal samples collected from 183 apparently healthy cattle, 180 pigs and 155 broilers throughout japan in 1999 were examined to determine the prevalence and antimicrobial susceptibility of salmonella. the isolation rates were 36.1% in broilers, 2.8% in pigs and 0.5% in cattle. s. enterica infantis was the most frequent isolate, found in 22.6% of broiler fecal samples. higher resistance rates were observed against oxytetracycline (82.0%), dihydrostreptomycin (77.9%), kanamycin (41. ... | 2009 | 19703311 |
| involvement of spi-2-encoded spic in flagellum synthesis in salmonella enterica serovar typhimurium. | spic encoded within salmonella pathogenicity island 2 on the salmonella enterica serovar typhimurium chromosome is required for survival within macrophages and systemic infection in mice. additionally, spic contributes to salmonella-induced activation of the signal transduction pathways in macrophages by affecting the expression of flic, a component of flagella filaments. here, we show the contribution of spic in flagellum synthesis. | 2009 | 19706157 |
| an outer membrane protease of the omptin family prevents activation of the citrobacter rodentium phopq two-component system by antimicrobial peptides. | the phopq two-component system of the intracellular pathogen salmonella enterica senses and controls resistance to alpha-helical antimicrobial peptides (amps) by regulating covalent modifications of lipid a. a homologue of the phopq operon was found in the genome of the murine enteric extracellular pathogen, citrobacter rodentium. here we report that c. rodentium phopq was apparently unable to mediate activation of target genes in the presence of alpha-helical amps. however, these amps activated ... | 2009 | 19708916 |
| cell yields and fermentation responses of a salmonella typhimurium poultry isolate at different dilution rates in an anaerobic steady state continuous culture. | the objectives of these studies were to determine cell yield and fermentation responses of a salmonella enterica serovar typhimurium poultry isolate using various dilution rates in steady state continuous culture incubations. s. enterica typhimurium cells were propagated in continuous cultures with a total volume of 0.50 l of luria bertani medium containing 0.1% glucose. dilution rates from 0.0125 to 1.44/h were used. cell protein concentration generally increased linearly with increased dilutio ... | 2009 | 19655268 |
| molecular typing of escherichia coli strains isolated from food in casablanca (morocco). | serotyping of o- and h- antigens is regarded as the gold standard in classification of e. coli for taxonomic and epidemiological purposes similar to the kaufmann-white scheme for salmonella enterica. molecular methods to replace or to support the serotyping were recently applied. using the molecular polymorphism of the somatic (o- antigen) gene rfb cluster and flagella (h- antigen) gene flic, 74 e. coli strains carrying the virulent genes isolated from food were characterised by polymerase chain ... | 2009 | 19656466 |
| protection of salmonella by ampicillin-resistant escherichia coli in the presence of otherwise lethal drug concentrations. | microbial systems have become the preferred testing grounds for experimental work on the evolution of traits that benefit other group members. this work, based on conceptual and theoretical models of frequency-dependent selection within populations, has proven fruitful in terms of understanding the dynamics of group beneficial or 'public goods' traits within species. here, we expand the scope of microbial work on the evolution of group-beneficial traits to the case of multi-species communities, ... | 2009 | 19656787 |
| global transcriptional response of salmonella enterica serovar typhi to anti-z66 antiserum. | recent studies have shown that flagella may modulate physiological processes by sensing environmental changes in temperature and moisture. when the z66(+) strain of salmonella enterica serovar typhi (s. typhi) was exposed to an antiserum against the z66 flagellar antigen, the fljba operon was deleted from a linear plasmid, leading to the unidirectional flagellar phase variation from fljb to flic. we hypothesized that flagella may serve as a sensor that responded to the antiserum by altering gene ... | 2009 | 19659726 |
| outbreak of salmonella enterica serotype muenster infections associated with goat's cheese, france, march 2008. | salmonella enterica serotype muenster (hereafter referred to as s. muenster) is rare in france and in europe. in france, a nationwide outbreak of gastrointestinal illness due to s. muenster occurred during march and april 2008. twenty-five laboratory-confirmed cases of s. muenster were documented by telephone using a trawling questionnaire. four patients were admitted to hospital and no death was recorded. among the 21 interviewed cases, 16 reported consumption of goat's cheese in the days prior ... | 2009 | 19660242 |
| macrophages influence salmonella host-specificity in vivo. | the generalist salmonella enterica serovar typhimurium causes disease in many animal species, but the closely related host-specific serovar typhi only causes disease in humans. typhi and typhimurium share major virulence loci; hence it is not known exactly why typhi does not cause disease in mice. we tested the hypothesis that macrophages contribute to salmonella host-specificity in mice. no significant difference in survival of the two serovars was observed in vitro in mouse macrophage cell lin ... | 2009 | 19660538 |
| [activity of ertapenem and ceftriaxone in the eradication of salmonella in a model of experimental peritonitis in mice]. | since the beginning of the 1990s, the prevalence of isolates of non-typhoidal salmonella species resistant to antimicrobial agents, including those commonly used for the treatment of salmonella infections such as fluoroquinolones, trimethoprim-sulfamethoxazole or beta-lactams, has increased substantially. infections caused by multidrug-resistant strains of non-typhoidal salmonella are now frequently encountered. in order to consider an appropriate role of ertapenem in salmonella-due intraabdomin ... | 2009 | 19662546 |
| characterization of lactobacillus spp. isolated from the feces of breast-feeding piglets. | lactobacillus spp., referred to as ij-1 and ij-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. according to the analyses of 16s rdna sequence, lactobacillus sp. ij-1 showed greater than 99% homology with lactobacillus reuteri dsm 20016(t), and lactobacillus sp. ij-2 had greater than 99% homology with the l. gasseri atcc 33323(t) and l. johnsonii atcc 33200(t). the ph changes in the culture media of lactobacillus sp. ij-1 and lactobacillus sp. ij-2 ... | 2009 | 19664551 |
| autonomous and flik-dependent length control of the flagellar rod in salmonella enterica. | salmonella flgg point mutations produce filamentous rod structures whose lengths are determined by flik. flik length variants produce rods with lengths proportional to the corresponding flik molecular size, suggesting that flik controls the length of not only the hook but also the rod by the same molecular mechanism. | 2009 | 19666714 |
| quantification of horizontal transmission of salmonella enterica serovar enteritidis bacteria in pair-housed groups of laying hens. | an important source of human salmonellosis is the consumption of table eggs contaminated with salmonella enterica serovar enteritidis. optimization of the various surveillance programs currently implemented to reduce human exposure requires knowledge of the dynamics of s. enteritidis infection within flocks. the aim of this study was to provide parameter estimates for a transmission model of s. enteritidis in laying-type chicken flocks. an experiment was carried out with 60 pairs of laying hens. ... | 2009 | 19666725 |
| autophagy genes protect against salmonella typhimurium infection and mediate insulin signaling-regulated pathogen resistance. | a conserved insulin-like pathway modulates both aging and pathogen resistance in caenorhabditis elegans. however, the specific innate effector functions that mediate this pathogen resistance are largely unknown. autophagy, a lysosomal degradation pathway, plays a role in controlling intracellular bacterial pathogen infections in cultured cells, but less is known about its role at the organismal level. we examined the effects of autophagy gene inactivation on salmonella enterica serovar typhimuri ... | 2009 | 19667176 |
| silica-antibiotic hybrid nanoparticles for targeting intracellular pathogens. | we investigated the capability of biodegradable silica xerogel as a novel carrier of antibiotic and the efficacy of treatment compared to that with the same dose of free drug against murine salmonellosis. the drug molecules (31%) entrapped in the sol-gel matrix remained in biologically active form, and the bactericidal effect was retained upon drug release. the in vitro drug release profiles of the gentamicin from the xerogel and that from the xerogel-polyethylene glycol (peg) were distinctly di ... | 2009 | 19667284 |
| polysaccharides derived from yamoa (funtumia elastica) prime gammadelta t cells in vitro and enhance innate immune responses in vivo. | yamoa (ground bark of funtumia elastica tree) is marketed and sold as a dietary supplement with anecdotal therapeutic effects in the treatment of asthma and hay fever. we determined that yamoa and yamoa-derived polysaccharides affected innate immunity, in part, by priming gammadelta t cells. gene expression patterns in purified bovine gammadelta t cells and monocytes induced by yamoa were similar to those induced by ultrapure lipopolysaccharide (ulps). in the presence of accessory cells, yamoa h ... | 2009 | 19671448 |
| an improved method to knock out the asd gene of salmonella enterica serovar pullorum. | an asd-deleted (deltaasd) mutant of salmonella enterica serovar pullorum (sp) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the red disruption system. the asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the cmr gene. based on the balanced lethal host-vector system, the phenotype of the deltaasd mutant was further defined. the improved method was simpler and more effective than pre ... | 2009 | 19672470 |
| use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the vibrio cholerae vaccine strain cvd 103-hgr. | live, attenuated vibrio cholerae vaccines can induce potent immune responses after only a single oral dose. the strategy of harnessing these strains to present antigens from heterologous pathogens to the mucosal immune system shows great promise. to fully realize this possibility, v. cholerae strains must be created that stably express antigens in vivo in sufficient quantity to generate an immune response. in vivo-induced promoters have been shown to increase the stability and immunogenicity of ... | 2009 | 19678844 |
| escherichia coli unsaturated fatty acid synthesis: complex transcription of the faba gene and in vivo identification of the essential reaction catalyzed by fabb. | although the unsaturated fatty acid (ufa) synthetic pathway of escherichia coli is the prototype of such pathways, several unresolved issues have accumulated over the years. the key players are the faba and fabb genes. earlier studies of faba transcription showed that the gene was transcribed from two promoters, with one being positively regulated by the fadr protein. the other weaker promoter (which could not be mapped with the technology then available) was considered constitutive because its ... | 2009 | 19679654 |
| antibody selection for immobilizing living bacteria. | we report a comparative study of the efficacy of immobilizing living bacteria by means of seven antibodies against bacterial surface antigens associated with salmonella enterica serovar typhimurium. the targeted bacterial antigens were cfa/i fimbriae, flagella, lipopolysaccharides (lps), and capsular f1 antigen. the best immobilization of s. typhimurium was achieved with the antibody against cfa/i fimbriae. the immobilization of bacteria using antiflagellin showed significant enhancement if the ... | 2009 | 19681578 |
| genomic diversity of citrate fermentation in klebsiella pneumoniae. | it has long been recognized that klebsiella pneumoniae can grow anaerobically on citrate. genes responsible for citrate fermentation of k. pneumoniae were known to be located in a 13-kb gene cluster on the chromosome. by whole genome comparison of the available k. pneumoniae sequences (mgh 78578, 342, and ntuh-k2044), however, we discovered that the fermentation gene cluster was present in mgh 78578 and 342, but absent in ntuh-k2044. in the present study, the previously unknown genome diversity ... | 2009 | 19682387 |
| role of a conserved prolyl residue (pro173) of mota in the mechanochemical reaction cycle of the proton-driven flagellar motor of salmonella. | the mota/b complex acts as the stator of the proton-driven bacterial flagellar motor. proton translocation through the stator complex is efficiently coupled with torque generation by the stator-rotor interactions. in salmonella enterica serovar typhimurium, the highly conserved pro173 residue of mota is close to the absolutely conserved asp33 residue of motb, which is believed to be a proton-binding site. pro173 is postulated to be involved in coupling proton influx to torque generation. however ... | 2009 | 19683537 |
| the s. typhimurium effector sope induces caspase-1 activation in stromal cells to initiate gut inflammation. | in the healthy intestinal mucosa, homeostasis between the immune system and commensal microflora prevents detrimental inflammatory responses. infection with acute enteropathogens such as salmonella enterica serovar typhimurium disturbs this homeostasis and triggers inflammation, but the underlying mechanisms are poorly understood. we found that bacterial delivery or ectopic expression of the s. typhimurium type iii effector protein sope, a known activator of host cellular rho gtpases, led to pro ... | 2009 | 19683679 |
| prognostic factors and clinical features of non-typhoid salmonella bacteremia in adults. | infections caused by non-typhoid salmonella (nts) have increased, and the increasing incidence of multidrug-resistant nts bacteremia in adult patients has also been noted. this study aimed to investigate the clinical and microbiological characteristics of nts bacteremia. | 2009 | 19686996 |
| molecular insights into farm animal and zoonotic salmonella infections. | salmonella enterica is a facultative intracellular pathogen of worldwide importance. infections may present in a variety of ways, from asymptomatic colonization to inflammatory diarrhoea or typhoid fever depending on serovar- and host-specific factors. human diarrhoeal infections are frequently acquired via the food chain and farm environment by virtue of the ability of selected non-typhoidal serovars to colonize the intestines of food-producing animals and contaminate the avian reproductive tra ... | 2009 | 19687040 |
| pspa family fusion proteins delivered by attenuated salmonella enterica serovar typhimurium extend and enhance protection against streptococcus pneumoniae. | pneumococcal surface protein a (pspa) is highly immunogenic and can induce a protective immune response against pneumococcal infection. pspa is divided into two major families based on serological variability: family 1 and family 2. to provide broad protection, pspa proteins from pneumococcal strains rx1 (family 1) and ef5668 (family 2) were combined to form two pspa fusion proteins, pspa/rx1-ef5668 and pspa/ef5668-rx1. each protein was fused to a type ii secretion signal and delivered by a reco ... | 2009 | 19687204 |
| msbb deletion confers acute sensitivity to co2 in salmonella enterica serovar typhimurium that can be suppressed by a loss-of-function mutation in zwf. | pathogens tolerate stress conditions that include low ph, oxidative stress, high salt and high temperature in order to survive inside and outside their hosts. lipopolysaccharide (lps), which forms the outer-leaflet of the outer membrane in gram-negative bacteria, acts as a permeability barrier. the lipid a moiety of lps anchors it to the outer membrane bilayer. the msbb enzyme myristoylates the lipid a precursor and loss of this enzyme, in salmonella, is correlated with reduced virulence and sev ... | 2009 | 19689794 |
| salmonella type iii secretion effector slrp is an e3 ubiquitin ligase for mammalian thioredoxin. | salmonella enterica encodes two virulence-related type iii secretion systems in salmonella pathogenicity islands 1 and 2, respectively. these systems mediate the translocation of protein effectors into the eukaryotic host cell, where they alter cell signaling and manipulate host cell functions. however, the precise role of most effectors remains unknown. using a genetic screen, we identified the small, reduction/oxidation-regulatory protein thioredoxin as a mammalian binding partner of the salmo ... | 2009 | 19690162 |
| rapid, sensitive, and specific detection of the o4 group of salmonella enterica by loop-mediated isothermal amplification. | the present study developed a loop-mediated isothermal amplification (lamp) assay that amplifies the fragments of o4 salmonella enterica-specific gene rfbj and evaluates the potential use in detection of salmonella enterica serovar typhimurium (st). the detection limit of the lamp assay was 10(3) cfu/ml, which was lower than that of the pcr assay with the same target gene (10(5) cfu/ml), confirmed by electrophoresis. the increased turbidity of the final products of lamp was also observed with mo ... | 2009 | 19630227 |
| paracoccidioides brasiliensis vaccine formulations based on the gp43-derived p10 sequence and the salmonella enterica flic flagellin. | paracoccidioidomycosis (pcm) is a systemic granulomatous disease caused by the dimorphic fungus paracoccidioides brasiliensis. anti-pcm vaccine formulations based on the secreted fungal cell wall protein (gp43) or the derived p10 sequence containing a cd4(+) t-cell-specific epitope have shown promising results. in the present study, we evaluated new anti-pcm vaccine formulations based on the intranasal administration of p. brasiliensis gp43 or the p10 peptide in combination with the salmonella e ... | 2009 | 19204092 |
| neonatal lipopolysaccharide and adult stress exposure predisposes rats to anxiety-like behaviour and blunted corticosterone responses: implications for the double-hit hypothesis. | the double-hit hypothesis posits that an early life genetic or environmental insult sets up a neural predisposition to psychopathology, which may emerge in the presence of a subsequent insult, or 'second hit' in later life. the current study assessed the effect of neonatal lipopolysaccharide (lps) exposure on anxiety-like behaviours in the adult wistar rat. rats were administered either lps (salmonella enterica, serotype enteritidis, 0.05 mg/kg, i.p.) or saline (equivolume) on days 3 and 5 of li ... | 2009 | 19524372 |
| respective roles of culturable and viable-but-nonculturable cells in the heterogeneity of salmonella enterica serovar typhimurium invasiveness. | the existence of salmonella enterica serovar typhimurium viable-but-nonculturable (vbnc) cells is a public health concern since they could constitute unrecognized sources of infection if they retain their pathogenicity. to date, many studies have addressed the ability of s. typhimurium vbnc cells to remain infectious, but their conclusions are conflicting. an assumption could explain these conflicting results. it has been proposed that infectivity could be retained only temporarily after entry i ... | 2009 | 19525274 |
| fecal shedding of salmonella spp among cattle admitted to a veterinary medical teaching hospital. | objective- to estimate the prevalence of fecal shedding of salmonella spp among bovine patients at a veterinary teaching hospital, to identify risk factors for fecal shedding of salmonella organisms, and to characterize the serotypes. design- retrospective cohort study. sample population- 5,398 hospitalized cattle. procedures- data were collected for all cattle admitted during an 11-year period. fecal shedding of salmonella spp was determined by means of standard bacteriologic culture. multivari ... | 2009 | 19527134 |
| rpoe fine tunes expression of a subset of ssrb-regulated virulence factors in salmonella enterica serovar typhimurium. | the survival of salmonella enterica within the intracellular host niche requires highly co-ordinated expression of virulence effectors predominantly regulated by the ssrab two-component regulatory system. s. enterica serovar typhimurium mutants lacking the ssrab genes are avirulent in mice, highlighting the importance of this regulatory system in vivo. mutants lacking the gene encoding the alternative sigma factor sigmae (rpoe) are also highly attenuated for intracellular survival, pointing to a ... | 2009 | 19245712 |
| salmonella enterica serovar typhimurium htra: regulation of expression and role of the chaperone and protease activities during infection. | htra is a bifunctional stress protein required by many bacterial pathogens to successfully cause infection. salmonella enterica serovar typhimurium (s. typhimurium) htra mutants are defective in intramacrophage survival and are highly attenuated in mice. transcription of htra in escherichia coli is governed by a single promoter that is dependent on sigma(e) (rpoe). s. typhimurium htra also possesses a sigma(e)-dependent promoter; however, we found that the absence of sigma(e) had little effect o ... | 2009 | 19246758 |
| polyserositis due to salmonellaenterica serovar enteritidis. | salmonella enterica serovar enteritidis (s. enteritidis), a non-typhoid salmonella, is an important emerging pathogen that usually causes gastroenteritis. here, we report polyserositis with right-sided pleural effusion and loculated collection of fluid in the peritoneum caused by s. enteritidis in a 60-year-old man from southern india. the patient was immunocompetent and did not have preceding gastroenteritis or any local structural abnormality. malnutrition and old age might have been the predi ... | 2009 | 19251293 |
| transcription factor function and promoter architecture govern the evolution of bacterial regulons. | evolutionary changes in ancestral regulatory circuits can bring about phenotypic differences between related organisms. studies of regulatory circuits in eukaryotes suggest that these modifications result primarily from changes in cis-regulatory elements (as opposed to alterations in the transcription factors that act upon these sequences). it is presently unclear how the evolution of gene regulatory circuits has proceeded in bacteria, given the rampant effects of horizontal gene transfer, which ... | 2009 | 19251636 |
| identification of escherichia coli and salmonella enterica organisms with reduced susceptibility to ceftriaxone from fecal samples of cows in dairy herds. | to estimate the relationship between therapeutic use of ceftiofur and recovery of escherichia coli and salmonella spp with reduced susceptibility to ceftriaxone from feces of dairy cattle. | 2009 | 19254152 |
| a multiplex real-time pcr for differential detection and quantification of salmonella spp., salmonella enterica serovar typhimurium and enteritidis in meats. | salmonella (s.) typhimurium and s. enteritidis are the major causative agents of food-borne illnesses worldwide. currently, a rapid detection system using multiplex real-time polymerase chain reaction (pcr) has been applied for other food-borne pathogens such as escherichia coli, staphylococcus aureus and streptococcus spp. a multiplex real-time pcr was developed for the simultaneous detection of salmonella spp., especially s. typhimurium and s. enteritidis, in beef and pork. for the specific an ... | 2009 | 19255523 |
| in vitro activity of gentamicin and amikacin against salmonella enterica serovar typhi: a search for a treatment regimen for typhoid fever. | the emergence of salmonella enterica serovar typhi isolates resistant to ciprofloxacin and 3rd-generation cephalosporins is a concern for physicians in developing countries. this study assessed the in vitro activity of gentamicin and amikacin against 464 s. entenca serovar typhi isolates obtained from blood of patients clinically suspected of enteric fever who attended the calcutta school of tropical medicine from 1991 to 2003. the isolates were sensitive to gentamicin and amikacin, showing mini ... | 2009 | 19554971 |
| development of a cell culture method to isolate and enrich salmonella enterica serotype enteritidis from shell eggs for subsequent detection by real-time pcr. | salmonella enterica serotype enteritidis is a major cause of nontyphoidal salmonellosis from ingestion of contaminated raw or undercooked shell eggs. current techniques used to identify salmonella serotype enteritidis in eggs are extremely laborious and time-consuming. in this study, a novel eukaryotic cell culture system was combined with real-time pcr analysis to rapidly identify salmonella serotype enteritidis in raw shell eggs. the system was compared to the standard microbiological method o ... | 2009 | 19561188 |
| virulotyping of salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in malaysia using multiplex-pcr. | the increased occurrence of salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. this study is aimed to detect the prevalence of twenty different virulence factors among salmonella enterica strains isolated from poultry and local indigenous vegetables in malaysia via an optimized, rapid and specific multiplex pcr assay. the assay encompasses a total of 19 salmonella pathogenicity islands genes and a quorum sensing gene (sdia) in three multiplex ... | 2009 | 19565351 |
| evaluation of quinolones for use in detection of determinants of acquired quinolone resistance, including the new transmissible resistance mechanisms qnra, qnrb, qnrs, and aac(6')ib-cr, in escherichia coli and salmonella enterica and determinations of wild-type distributions. | fluoroquinolone resistance in members of the enterobacteriaceae family is mostly due to mutations in the quinolone resistance-determining regions of the topoisomerase genes. however, transferable genes encoding quinolone resistance have recently been described. the current methods for susceptibility testing are not adapted to the detection of new resistance determinants, which confer low levels of resistance. the aim of this study was to compare the ability of the screening of the different quin ... | 2009 | 19571019 |
| who global salm-surv external quality assurance system for serotyping of salmonella isolates from 2000 to 2007. | an international external quality assurance system (eqas) for the serotyping of salmonella species was initiated in 2000 by who global salm-surv to enhance the capacity of national reference laboratories to obtain reliable data for surveillance purposes worldwide. seven eqas iterations were conducted between 2000 and 2007. in each iteration, participating laboratories submitted serotyping results for eight salmonella isolates. a total of 249 laboratories in 96 countries participated in at least ... | 2009 | 19571024 |
| association of virulence plasmid and antibiotic resistance determinants with chromosomal multilocus genotypes in mexican salmonella enterica serovar typhimurium strains. | bacterial genomes are mosaic structures composed of genes present in every strain of the same species (core genome), and genes present in some but not all strains of a species (accessory genome). the aim of this study was to compare the genetic diversity of core and accessory genes of a salmonella enterica subspecies enterica serovar typhimurium (typhimurium) population isolated from food-animal and human sources in four regions of mexico. multilocus sequence typing (mlst) and macrorestriction f ... | 2009 | 19573249 |
| crystallization and preliminary x-ray characterization of a glycerol dehydrogenase from the human pathogen salmonella enterica serovar typhimurium. | glycerol dehydrogenase (glda) encoded by the stm4108 gene (glda) has been related to the synthesis of hila, a major transcriptional regulator that is responsible for the expression of invasion genes in the human pathogen salmonella enterica serovar typhimurium. single colourless crystals were obtained from a recombinant preparation of glda overexpressed in escherichia coli. they belonged to space group p222(1), with unit-cell parameters a = 127.0, b = 160.1, c = 665.2 a. the crystals contained a ... | 2009 | 19574643 |
| listeria monocytogenes membrane trafficking and lifestyle: the exception or the rule? | listeria monocytogenes is an intracellular bacterial pathogen that promotes its internalization within nonprofessional phagocytes by interacting with specific host cell receptors. l. monocytogenes resides transiently in a membrane-bound compartment before escaping into the host cell cytosol where bacterial proliferation takes place. actin-based motility then promotes cell-to-cell pathogen spread. extensive studies on cytoskeleton rearrangements, membrane trafficking, and other events have establ ... | 2009 | 19575658 |
| comparative analysis of salmonella enterica serovar enteritidis mutants with a vaccine potential. | if any new live salmonella vaccine is introduced in the future, it is quite probable that detailed characterisation of its attenuation will be required. in this study we therefore compared 34 isogenic mutants of s. enteritidis in aroa, arod, gale, ssra, ssea, phop, rpos, ompr, htra, clpp, lon, rfal, rfag, rfac, hfq, sodci, hila, sipa, avra, sopb, sopa, sope, sifa, shda, flic, fur, rela, spot, rel-spot, misl, rmba, stm4258, stm4259 and spvbc genes for their resistance to stresses likely to be exp ... | 2009 | 19577637 |
| analysis of pools of targeted salmonella deletion mutants identifies novel genes affecting fitness during competitive infection in mice. | pools of mutants of minimal complexity but maximal coverage of genes of interest facilitate screening for genes under selection in a particular environment. we constructed individual deletion mutants in 1,023 salmonella enterica serovar typhimurium genes, including almost all genes found in salmonella but not in related genera. all mutations were confirmed simultaneously using a novel amplification strategy to produce labeled rna from a t7 rna polymerase promoter, introduced during the construct ... | 2009 | 19578432 |
| clustering analysis of salmonella enterica serovar typhi isolates in korea by pfge, ribotying, and phage typing. | salmonella enterica serovar typhi is a gram-negative bacterium causing the acute febrile disease typhoid fever. in korea from 2004 to 2006, a total of 51 salmonella typhi isolates were identified in stool and blood from healthy carriers and patients with or without overseas travel histories. in this study, antibiogram, pulsed-field gel electrophoresis (pfge), and automated ribotyping were performed as molecular epidemiological methods with phage typing as a classical subtyping tool of the isolat ... | 2009 | 19580446 |
| molecular characterization of multidrug-resistant strains of salmonella enterica serotype typhimurium and monophasic variant (s. 4,[5],12:i:-) isolated from human infections in italy. | salmonella enterica serovar typhimurium (stm) represents the prevalent cause of foodborne gastroenteritis in italy with the majority of isolates exhibiting multidrug resistance. a resistant pattern that includes ampicillin (a), streptomycin (s), sulfonamide (su), and tetracycline (t) (assut) but lacks resistance to chloramphenicol (c) has recently emerged in italy among strains of stm and of its monophasic variant, s. enterica subspecies enterica serovar s. 4,[5],12:i:-. with the aim to evaluate ... | 2009 | 19580448 |
| salmonella enterica serovar typhimurium in mauritius linked to consumption of marlin mousse. | we report the first outbreak of salmonellosis caused by consumption of contaminated marlin mousse. between 29 october and 5 november 2008, at least 53 persons developed diarrheal illness, all with a history of eating marlin mousse. salmonella spp. that did not produce gas from glucose was isolated from stools of 26 affected patients and blood culture from one patient. salmonella sp. isolates with the same phenotype were isolated in three samples of marlin mousse manufactured on 27 october 2008. ... | 2009 | 19580452 |
| inhibition of intracellular growth of salmonella enterica serovar typhimurium in tissue culture by antisense peptide-phosphorodiamidate morpholino oligomer. | two types of phosphorodiamidate morpholino oligomers (pmos) were tested for inhibition of growth of salmonella enterica serovar typhimurium. both pmos have the same 11-base sequence that is antisense to the region near the start codon of acpp, which is essential for lipid biosynthesis and viability. to the 3' end of each is attached the membrane-penetrating peptide (rxr)4xb (r, x, and b indicate arginine, 6-aminohexanoic acid, and beta-alanine, respectively). one peptide-pmo (acpp ppmo) has no c ... | 2009 | 19581453 |
| impact of therapeutic treatment with beta-lactam on transfer of the bla(ctx-m-9) resistance gene from salmonella enterica serovar virchow to escherichia coli in gnotobiotic rats. | the conjugative transfer of the plasmid carrying the bla(ctx-m-9) gene from salmonella enterica serovar virchow isolated from a chicken farm to a recipient escherichia coli strain was evaluated in vitro and in axenic rats inoculated with both strains, with or without selective pressure due to therapeutic doses of cefixime. the transfer of the bla(ctx-m-9) gene of s. enterica serovar virchow to e. coli was confirmed in vitro, at a low frequency of 5.9 x 10(-8) transconjugants/donors. this transfe ... | 2009 | 19581466 |
| genome-wide identification of novel genomic islands that contribute to salmonella virulence in mouse systemic infection. | salmonella pathogenicity islands are inserted into the genome by horizontal gene transfer and are required for expression of full virulence. here, we performed trna scanning of the genome of salmonella enterica serovar typhimurium and compared it with that of nonpathogenic escherichia coli in order to identify genomic islands that contribute to salmonella virulence. using deletion analysis, we identified four genomic islands that are required for virulence in the mouse infection model. one of th ... | 2009 | 19583791 |
| salmonella wien from gastroenteritis cases encountered in mangalore, india: a report of 10 cases and review of the literature. | ten cases of gastroenteritis due to s. wien were reported from a tertiary care hospital in mangalore, in the month of april 2008. biochemically, it resembles s. paratyphi b or s. typhimurium. serotyping was done at the central research institute, kasauli, to confirm the identity. the food source is more likely to be chicken or some poultry products. all the 10 cases recovered with symptomatic treatment including antimicrobials like fluoroquinolones. to the best of our knowledge, no documented re ... | 2009 | 19584490 |
| food poisoning due to salmonella enterica serotype weltevreden in mangalore. | an outbreak of food poisoning due to salmonella enterica serotype weltevreden ( s.weltevreden ) involving 34 students has been reported from a tertiary care hospital in mangalore. the symptoms developed 8-10 hours, after consuming a non- vegetarian dish, probably fish, from an outside caterer. the identity of the organism was confirmed at central research institute, kasauli. this report emphasizes the geographical distribution of this organism in the coastal karnataka region. s.weltevreden may b ... | 2009 | 19584510 |
| integrated microfluidic electrochemical dna sensor. | effective systems for rapid, sequence-specific nucleic acid detection at the point of care would be valuable for a wide variety of applications, including clinical diagnostics, food safety, forensics, and environmental monitoring. electrochemical detection offers many advantages as a basis for such platforms, including portability and ready integration with electronics. toward this end, we report the integrated microfluidic electrochemical dna (imed) sensor, which combines three key biochemical ... | 2009 | 19586008 |
| salmonella enterica serovar typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by lactobacillus rhamnosus gg. | physiological stressors may alter susceptibility of the host intestinal epithelium to infection by enteric pathogens. in the current study, cytotoxic effect, adhesion and invasion of salmonella enterica serovar typhimurium (s. typhimurium) to caco-2 cells exposed to thermal stress (41 degrees c, 1 h) was investigated. probiotic bacteria have been shown to reduce interaction of pathogens with the epithelium under non-stress conditions and may have a significant effect on epithelial viability duri ... | 2009 | 19589170 |
| the enzyme phosphoglucomutase (pgm) is required by salmonella enterica serovar typhimurium for o-antigen production, resistance to antimicrobial peptides and in vivo fitness. | the enzyme phosphoglucomutase (pgm) catalyses the interconversion of glucose 1-phosphate and glucose 6-phosphate and contributes to glycolysis and the generation of sugar nucleotides for biosynthesis. to assess the role of this enzyme in the biology of the pathogen salmonella enterica serovar typhimurium we have characterized a pgm deletion mutant in strain sl1344. compared to sl1344, sl1344 pgm had impaired growth in vitro, was deficient in the ability to utilize galactose as a carbon source an ... | 2009 | 19589833 |
| conjugative plasmid transfer between salmonella enterica newport and escherichia coli within the gastrointestinal tract of the lesser mealworm beetle, alphitobius diaperinus (coleoptera: tenebrionidae). | the objective of this study was to determine if conjugative transfer of antimicrobial resistance plasmids could occur between donor and recipient bacteria within the gastrointestinal tract of lesser meal-worm beetles, a common pest in poultry production facilities. in 3 replicate studies (n = 40 overall), beetles were allowed to feed for 2 h on brain heart infusion agar inoculated with a multidrug-resistant salmonella enterica serotype newport strain (sn11 that carried plasmid replicons a/c and ... | 2009 | 19590068 |
| potential applicability of chymotrypsin-susceptible microcin j25 derivatives to food preservation. | microcin j25 (mccj25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. mccj25 is active against such food-borne disease-causing pathogens as salmonella spp., shigella spp., and escherichia coli, including e. coli o157:h7 and non-o157 strains. mccj25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. mccj25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the ... | 2009 | 19592527 |
| deletion of sodci and spvbc in salmonella enterica serovar enteritidis reduced its virulence to the natural virulence of serovars agona, hadar and infantis for mice but not for chickens early after infection. | salmonella enterica serovars typhimurium, enteritidis, dublin, choleraesuis or gallinarum can colonise liver and spleen in particular hosts while infections with serovars infantis, agona, hadar, etc. are usually limited to gastrointestinal tract. reasons for this behavior are unknown, although it has been shown that sodci and spv genes exhibit a strict distribution between more and less virulent serovars and they influence salmonella virulence. however to what extent the presence or absence of t ... | 2009 | 19595520 |
| oral vaccination with lcrv from yersinia pestis kim delivered by live attenuated salmonella enterica serovar typhimurium elicits a protective immune response against challenge with yersinia pseudotuberculosis and yersinia enterocolitica. | the use of live recombinant attenuated salmonella vaccines (rasv) synthesizing yersinia proteins is a promising approach for controlling infection by yersinia species. in this study, we constructed attenuated salmonella strains which synthesize a truncated form of lcrv, lcrv196 and evaluated the immune response and protective efficacy elicited by these strains in mice against two other major species of yersinia: yersinia pseudotuberculosis and yersinia enterocolitica. surprisingly, we found that ... | 2009 | 19596407 |
| protection of mice from brucella infection by immunization with attenuated salmonella enterica serovar typhimurium expressing a l7/l12 and bls fusion antigen of brucella. | this study describes the potential use of attenuated salmonella enterica serovar typhimurium strains (s. typhimurium) to express and deliver a l7/l12 and bls fusion antigen of brucella as a vaccination strategy to prevent brucella infection in mice. s. typhimurium x4072 that contained a ptrc99a-bls-l7/l12 plasmid, designated x4072bl, can deliver a l7/l12 and bls fusion antigen expressed by the bacterium itself, while s. typhimurium x4550 that contained an asd-pvax1-bls-l7/l12 (asd-pbl) plasmid, ... | 2009 | 19596411 |
| toll-like receptor 5-dependent regulation of inflammation in systemic salmonella enterica serovar typhimurium infection. | salmonella enterica, a gram-negative pathogen, causes a spectrum of human infections including enterocolitis and typhoid fever. we previously showed that salmonella flagellin played a role in suppressing intestinal mucosal inflammation in a murine model of acute enterocolitis. in this study, we examined the role of flagellin in the typhoid-like systemic murine salmonella infection by measuring bacterial proliferation, inflammation, leukocyte recruitment, and cellular apoptosis in peyer's patches ... | 2009 | 19596770 |
| mechanisms of resistance in nontyphoidal salmonella enterica strains exhibiting a nonclassical quinolone resistance phenotype. | nontyphoidal salmonella enterica strains with a nonclassical quinolone resistance phenotype were isolated from patients returning from thailand or malaysia to finland. a total of 10 isolates of seven serovars were studied in detail, all of which had reduced susceptibility (mic > or = 0.125 microg/ml) to ciprofloxacin but were either susceptible or showed only low-level resistance (mic < or = 32 microg/ml) to nalidixic acid. phenotypic characterization included susceptibility testing by the agar ... | 2009 | 19596880 |
| report of neonatal meningitis due to salmonella enterica serotype agona and review of breast milk-associated neonatal salmonella infections. | we present the first documented case of salmonella enterica serotype agona meningitis in a 6-day-old baby. s. enterica serotype agona was isolated concurrently from infant cerebrospinal fluid and parental fecal samples, and salmonella was isolated from breast milk. the role of breast milk in transmission of salmonella enterica is discussed. | 2009 | 19605582 |
| oral immunization with attenuated salmonella enterica serovar typhimurium encoding cryptosporidium parvum cp23 and cp40 antigens induces a specific immune response in mice. | attenuated salmonella enterica serovar typhimurium vaccine strain sl3261 was used as an antigen delivery system for the oral immunization of mice against two cryptosporidium parvum antigens, cp23 and cp40. each antigen was subcloned into the ptech1 vector system, which allows them to be expressed as fusion proteins with highly immunogenic fragment c of tetanus toxin under the control of the anaerobically inducible nirb promoter. the recombinant vector was introduced into salmonella typhimurium v ... | 2009 | 19605593 |
| a strand-specific rna-seq analysis of the transcriptome of the typhoid bacillus salmonella typhi. | high-density, strand-specific cdna sequencing (ssrna-seq) was used to analyze the transcriptome of salmonella enterica serovar typhi (s. typhi). by mapping sequence data to the entire s. typhi genome, we analyzed the transcriptome in a strand-specific manner and further defined transcribed regions encoded within prophages, pseudogenes, previously un-annotated, and 3'- or 5'-untranslated regions (utr). an additional 40 novel candidate non-coding rnas were identified beyond those previously annota ... | 2009 | 19609351 |
| addressing the effects of salmonella internalization in host cell signaling on a reverse-phase protein array. | through acute enteric infection, salmonella invades host enterocytes and reproduces intracellularly into specialized vacuolae. this involves changes in host cell signaling elicited by bacterial proteins delivered via type iii secretion systems (ttss). one of the two ttsss of salmonella enterica serovar typhimurium encoded by the salmonella pathogenicity island-1, triggers bacterial internalization. among the effector proteins translocated by this ttss, the gtpase modulator sope/e2 and the phosph ... | 2009 | 19609973 |
| simultaneous identification of seven foodborne pathogens and escherichia coli (pathogenic and nonpathogenic) using capillary electrophoresis-based single-strand conformation polymorphism coupled with multiplex pcr. | the objective of this study was to develop a novel technique for parallel analysis of eight important foodborne microbes using capillary electrophoresis-based single-strand conformation polymorphism (ce-sscp) coupled with multiplex pcr. specific primers for multiplex pcr amplification of the 16s rrna gene were designed, corresponding to eight species of bacteria, including escherichia coli, clostridium perfringens, campylobacter jejuni, salmonella enterica, listeria monocytogenes, vibrio parahae ... | 2009 | 19610337 |
| prevalence of salmonella in vegetables from mexico. | the present study is an overview of the role of vegetables as a transmission vehicle of salmonella in mexico. one hundred samples of each of 17 different vegetables were analyzed during a period of 18 months. salmonella was isolated from 98 samples. salmonella enterica serovar typhimurium was isolated from the highest percentage of samples with typeable salmonella isolates (23.9%), followed by s. enterica subsp. arizonae and salmonella choleraesuis each from 16.9%, salmonella gallinarum from 11. ... | 2009 | 19610340 |
| real-time pcr as a tool for detection of pathogenic bacteria on contaminated food contact surfaces by using a single enrichment medium. | foodborne pathogens such as escherichia coli o157:h7, listeria monocytogenes, and salmonella can easily transfer from food to food contact surfaces. methods for rapid detection of these pathogenic bacteria are important in order to avoid contamination of food. here, we describe the detection and quantification of bacterial pathogens on contaminated surfaces by real-time pcr (rt-pcr) and culture methods using a single enrichment medium. surfaces of wood, polypropylene, and stainless steel were in ... | 2009 | 19610347 |
| the interaction of the antimicrobial peptide cleap-2 and the bacterial membrane. | chicken liver expressed antimicrobial peptide-2 (cleap-2) is known to have killing activities against salmonella spp., but the mechanism by which killing occurs remains to be elucidated. the ability of cleap-2 to disrupt the outer membrane of several salmonella spp. was assessed using the fluorescent probe n-phenyl-1-naphthylamine (npn). a rapid dose-dependent permeabilization of the outer membranes of salmonella enterica serovar typhimurium phop, and s. enterica serovar typhimurium sl1344 was o ... | 2009 | 19615343 |
| application of an automated immunomagnetic separation-enzyme immunoassay for the detection of salmonella enterica subspecies enterica from poultry environmental swabs. | an automated immunomagnetic separation (ims) and enzyme immunoassay (eia) was applied to the detection of salmonella enterica subspecies enterica serotypes from poultry environmental samples. the analytical sensitivity and specificity of the ims-eia for 46 s. enterica serotypes and 33 non-salmonellae isolates belonging to 21 different genera were 91.3% and 90.9%, respectively. from post enrichment s. enterica cultures, the limit of detection of the assay was estimated at 10(4)-10(6) cfu/ml. appl ... | 2009 | 19616586 |
| fatty acid profiling and proteomic analysis of salmonella enterica serotype typhimurium inactivated with supercritical carbon dioxide. | non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. in particular, since supercritical carbon dioxide (sc-co2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. although sc-co2 has been applied to many microorganisms, the inactivation of microb ... | 2009 | 19616865 |
| characterization of salmonella enterica serotype typhimurium from outpatients of 28 hospitals in henan province in 2006. | to characterize the diarrheal patients with salmonella typhimurium (s. typhimurium) infections and to set up the first baseline for s. typhimurium pulsed-field gel electrophoresis (pfge) patterns in henan province, thus laying a foundation for comprehensive surveillance of salmonella in human as well as foods. | 2009 | 19618691 |
| role of nod1 in mucosal dendritic cells during salmonella pathogenicity island 1-independent salmonella enterica serovar typhimurium infection. | recent advances in immunology have highlighted the critical function of pattern-recognition molecules (prms) in generating the innate immune response to effectively target pathogens. nod1 and nod2 are intracellular prms that detect peptidoglycan motifs from the cell walls of bacteria once they gain access to the cytosol. salmonella enterica serovar typhimurium is an enteric intracellular pathogen that causes a severe disease in the mouse model. this pathogen resides within vacuoles inside the ce ... | 2009 | 19620349 |
| combining capillary electrophoresis with laser-induced fluorescence detection for the analysis of escherichia coli lysates. | with the goal of improving water quality control, in this study we combined ce-lif for the analysis of escherichia coli lysates under discontinuous conditions. prior to injecting a large-volume protein sample, a plug of 0.2% w/v sds was injected into the capillary filled with 2.0 m tris-borate (tb) solution (ph 10.3). during the courses of the process stacking, proteins migrated against the electroosmotic flow (eof) and entered a 1.7% w/v poly(ethylene oxide) solution that has been prepared in 2 ... | 2009 | 19621366 |
| [construction of a dual-promoter expression plasmid delivered by salmonella choleraesuis c500]. | salmonella choleraesuis c500 strain is an attenuated vaccine preventing piglet from paratyphoid and can also be used as a live vector of other dna vaccines. through mucosal immunization, immune response to specific antigens carried by it can be induced. to enhance the immune efficiency of dna vaccine it carried, promoter ptrc was inserted into the down stream of the human cytomegalovirus (cmv) immediate early promoter of eukaryotic expression plasmid pegfp-c1. then transcription terminator rrnbt ... | 2009 | 19621572 |
| [construction of attenuated salmonella choleraesuis vaccine strain expressing recombinant antigen of shiga-like toxin escherichia coli]. | to construct an attenuated salmonella choleraesuis vaccine strain expressing the gene slt-iieb and fedf of shiga-like toxin escherichia coli (sltec) o138 with balanced lethal system. | 2009 | 19621641 |
| improvement of an acid phosphatase/dhap-dependent aldolase cascade reaction by using directed evolution. | to enhance the phosphorylating activity of the bacterial nonspecific acid phosphatase from salmonella enterica ser. typhimurium lt2 towards dihydroxyacetone (dha), a mutant library was generated from the native enzyme. three different variants that showed enhanced activity were identified after one round of eppcr. the single mutant v78l was the most active and showed an increase in the maximal dhap concentration to 25 % higher than that of the wild-type enzyme at ph 6.0. this variant is 17 times ... | 2009 | 19623595 |
| meeting on establishment of consortium to study invasive salmonelloses in sub-saharan africa. | 2009 | 19624911 | |
| role of salmonella enterica lipopolysaccharide in activation of dendritic cell functions and bacterial containment. | in contrast to nonpathogenic bacteria, the gram-negative pathogen salmonella enterica is not eradicated, but persists in murine dendritic cells (dc). the molecular basis of this phenotype is unknown. we set out to characterize bacterial and dc functions that are involved in salmonella persistence. our data prove that neither bacterial nor host cell de novo protein biosynthesis is required for salmonella persistence in dc. we identified the salmonella o-ag of the lps of salmonella as an important ... | 2009 | 19625639 |
| turtle-associated salmonella septicaemia and meningitis in a 2-month-old baby. | a severe case of reptile-associated salmonellosis which caused septicaemia and meningitis in a 2-month-old baby is reported. the infrequent serotype salmonella enterica subsp.(i) enterica serotype abony (4,5 : b : enx) was detected in the human sample as well as in the pet turtle's faeces. the importance of regulation and public awareness is highlighted. | 2009 | 19528160 |
| salmonella expressing a t-cell epitope from sendai virus are able to induce anti-infection immunity. | bacterial fimbriae can accept foreign peptides and display them on the cell surface. a highly efficient gene replacement method was used to generate peptide vaccines based on salmonella enterica subsp. enterica serovar typhimurium lt2. dna encoding an epitope from sendai virus, sv9 (sendai virus nucleoprotein peptide 324-332, fapgnypal), which is known to induce cytotoxic t lymphocytes, was incorporated into the gene encoding agfa (the major subunit protein of thin aggregative fimbriae of salmon ... | 2009 | 19528162 |
| accelerated type iii secretion system 2-dependent enteropathogenesis by a salmonella enterica serovar enteritidis pt4/6 strain. | salmonella enterica subsp. i serovars typhimurium and enteritidis are major causes of enteric disease. the pathomechanism of enteric infection by serovar typhimurium has been studied in detail. serovar typhimurium employs two pathways in parallel for triggering disease, i.e., the "classical" pathway, triggered by type iii secretion system 1 (ttss-1), and the "alternative" pathway, mediated by ttss-2. it had remained unclear whether these two pathways would also explain the enteropathogenesis of ... | 2009 | 19528213 |
| proteomic investigation of the time course responses of raw 264.7 macrophages to infection with salmonella enterica. | to investigate the extent to which macrophages respond to salmonella infection, we infected raw 264.7 macrophages with salmonella enterica serotype typhimurium and analyzed macrophage proteins at various time points following infection by using a global proteomic approach. a total of 1,006 macrophage and 115 salmonella proteins were identified with high confidence. most of the salmonella proteins were observed in the late stage of the infection time course, which is consistent with the fact that ... | 2009 | 19528222 |
| characterization of the plasmid-borne quinolone resistance gene qnrb19 in salmonella enterica serovar typhimurium. | a qnrb19 gene variant, carried by an incl/m-like plasmid, was detected in a multidrug salmonella enterica serovar typhimurium human strain with reduced susceptibility to ciprofloxacin. the genetic environment around the gene was fully sequenced (20 kb). a large gene cluster, containing the aph, qnrb19, and blashv-12-like resistance genes, is inserted inside a tn3 transposon. | 2009 | 19528272 |