Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| clinical epidemiology of symptomatic primary herpetic infection in children. a study of 50 cases. | we have studied a series of 50 children with clinical primary herpetic infection during 1975-1985. our data confirm: absence of sex differences and seasonal variations, a peak age of incidence between 6 and 24 months, a prevalence of patients of lower social status, evidence of recurrent herpes labialis as the most frequent source of infection, and frequency of herpes simplex virus 1 gingivostomatitis. the relevant findings of this study were as follows: herpes simplex virus 2 was isolated in 10 ... | 1987 | 3031926 |
| transcriptional induction of proto-oncogene fos by hsv-2. | hsv-2 induces the transcription of c-fos gene in both human (diploid) cell strains and mouse (heteroploid) cell lines. hsv-1 is incapable of such induction. the induction of transcription is rapid and transient in both cell types. the product of transcriptional induction of c-fos in mouse cells is mainly a 4.5 kb fos mrna along with lesser amount of a 2.2 kb fos mrna, while in human cells the 4.5 kb mrna is the only detectable product. both the 4.5 kb and 2.2 kb fos mrnas are mature gene product ... | 1987 | 3032180 |
| inhibitory effect of azone (1-dodecylazacycloheptan-2-one) on herpes simplex viruses. in vivo and in vitro studies. | enhanced activity of antiviral agents, when mixed with a penetration enhancer like azone (1-dodecylazacycloheptan-2-one) in topical preparations, is well-recognized. these studies were undertaken to investigate whether azone, per se, has any activity against herpes simplex (hsv) type 1 and type 2 viruses. when hsv-1 and hsv-2 were mixed with 5, 10 and 20% azone, there was a 1-4.5 log10 decrease in virus titers. the efficacy of 5 and 10% azone applied topically was evaluated during the treatment ... | 1987 | 3032527 |
| effect of dehydrating agents on dna organization in herpes viruses. | with routine procedures of epon- or gma-embedding and a stain specific for dna, the nucleoid of mature herpes simplex virus-type 1 (hsv-1) assumes the well-known form of a short, compact, hollow cylinder or torus. a new, more complex organization of dna filaments in encapsidated hsv-1 was found in infected cells after aldehyde fixation, methanol dehydration, and lowicryl embedment. we have determined that it is the use of methanol as dehydrating agent that permits visualization of this internal ... | 1987 | 3033063 |
| suppression of delayed type hypersensitivity to herpes simplex virus type 1 following immunization with anti-idiotypic antibody: an example of split tolerance. | intraperitoneal (i.p.) immunization with herpes simplex virus type 1 (hsv-1) or an anti-idiotypic antibody (anti-id c) prepared against a monoclonal antibody specific for glycoprotein c of hsv-1, tolerizes mice for an hsv-1 delayed type hypersensitivity (dth) response. this tolerization could be adoptively transferred to naive x-irradiated mice by splenic t cells, and was specific for hsv-1 dth. thus, dth-tolerized mice responded to vaccinia virus or hsv-2 challenge, while remaining tolerized fo ... | 1987 | 3033128 |
| virulence is not conserved in recombinants between herpes simplex virus types 1 and 2. | the virulence of 31 herpes simplex virus type 1 x herpes simplex virus type 2 intertypic recombinants was determined following intraperitoneal inoculation into cba mice. only eight of the recombinants killed any of the mice and of these, only one recombinant was as virulent as its type 2 parent, the other seven recombinants being intermediate between their type 1 and type 2 parental viruses in virulence. these results indicate that most heterotypic combinations are attenuated independently of th ... | 1987 | 3033143 |
| processing of the herpes simplex virus type 2 glycoprotein gg-2 results in secretion of a 34,000-mr cleavage product. | herpes simplex virus type 2 glycoprotein gg-2 undergoes a cleavage event during its synthesis and processing. the focus of this report is on the detection and fate of the small-molecular-weight component of gg-2, designated the 34k component. in cultures containing the inhibitor monensin, a 31k component accumulated within infected cells. in contrast, the intracellular accumulation of this 31k precursor was not detected in cultures grown in the absence of the inhibitor. however, the 34k componen ... | 1987 | 3033302 |
| recurrences after oral and genital herpes simplex virus infection. influence of site of infection and viral type. | we prospectively followed 39 adults with concurrent primary herpes simplex virus (hsv) infection (12 with hsv type 1 and 27 with hsv type 2) of the oropharynx and genitalia, caused by the same virus in each person, to evaluate the influence of viral type (hsv-1 vs. hsv-2) and site of infection (oropharyngeal vs. genital) on the frequency of recurrence. the subsequent recurrence patterns of hsv infection differed markedly according to viral type and anatomical site. oral-labial recurrences develo ... | 1987 | 3033506 |
| inhibition of cell-associated herpes simplex virus type 2 glycoproteins by delta 9-tetrahydrocannabinol. | this study was conducted to define the effect of micromolar concentrations of delta 9-tetrahydrocannabinol (delta 9-thc) on the biosynthesis and expression of herpes simplex virus type 2 (hsv2)-specified glycoproteins. dose-related reductions in all species of virus glycoproteins were recorded by one-dimensional sds-polyacrylamide gel electrophoresis (sds-page) and autoradiography of [14c]glucosamine-labeled infected vero cells treated with 10(-7) to 10(-5) m delta 9-thc. a drug dose-related dep ... | 1987 | 3033681 |
| trifluorothymidine: potential non-invasive diagnosis of herpes simplex infection using 19f nuclear magnetic resonance in a murine hepatitis model. | trifluorothymidine (tft) is known to be concentrated in herpes simplex virus (hsv) infected cells in vitro in the form of phosphorylated derivatives. we studied a murine hepatitis model of hsv infection to determine whether this in vitro observation would also be demonstrable in vivo. following i.v. injection of 100 or 160 mg/kg tft, tft was found in significantly higher concentrations in the livers of hsv-2 infected mice than in the livers of uninfected mice, mice infected with murine hepatitis ... | 1987 | 2832432 |
| antibodies to simian vacuolating virus 40 in bladder cancer patients. | the occurrence of antibodies to herpes simplex virus type 2 (hsv-2) and simian vacuolating virus 40 (sv40) (two presumptive oncogenic viruses) was investigated by the technique of enzyme-linked immunosorbent assay in 233 patients with bladder cancer (200 males and 33 females) and in 466 controls (400 males and 66 females). the age of both the bladder cancer patients and controls varied from 55 to 65 years. a statistically significant association between bladder cancer and antibodies to sv40 was ... | 1987 | 2834853 |
| role of herpes simplex virus type-2 and human papilloma virus in penile cancers in singapore. | to detect the presence of herpes simplex virus type 2 (hsv-2) and human papilloma virus (hpv), common structural antigens in penile cancers, 20 squamous cell carcinomas were studied by immunohistochemical methods. all specimens were from singaporeans ranging in age from 44 to 69 years. none of the specimens contained hsv-2 and hpv common structural antigens. the reasons for the absence of hpv common antigen were discussed. the significance of the association of hpv with penile cancers is uncerta ... | 1987 | 2829697 |
| nucleotide sequence changes in thymidine kinase gene of herpes simplex virus type 2 clones from an isolate of a patient treated with acyclovir. | to identify the nucleotide changes that occur in drug-induced thymidine kinase (tk) mutants of herpes simplex virus type 2 (hsv-2), we compared the nucleotide sequences of the tk genes of two mutant hsv-2 clones isolated from a patient who had been treated with acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] with the nucleotide sequence of the parental tk+ hsv-2(8703) strain isolated from the same patient. one of the mutants, tk-altered (tka) hsv-2(9637), was acv resistant but induced the inco ... | 1987 | 2829709 |
| inactivation of herpes simplex virus types 1 and 2 by synthetic histidine peptides. | synthetic homologous histidine peptides were found to directly and irreversibly inactivate herpes simplex virus types 1 and 2 (hsv-1 and hsv-2). the inactivation, which occurred within 1 min of virus exposure to the drug, was independent of temperature but dependent upon the ph and molecular size of the polypeptide. poly-l-histidine consisting of 24 residues (his-24), with a molecular weight (m.w.) of 3,310, inactivated greater than 99% of the virus present at ph 6.0 and 62% at ph 5.0. poly-l-hi ... | 1987 | 2829711 |
| delta-9-tetrahydrocannabinol inhibits the splenocyte proliferative response to herpes simplex virus type 2. | the present investigation was undertaken to determine the effect of in vivo delta-9-tetrahydrocannabinol (delta-9-thc) treatment on immune responsiveness to secondary exposure to herpes simplex virus type 2 (hsv2) antigens in vitro. a splenocyte proliferative assay, employing hsv2-infected mouse embryo fibroblasts as target cells, was used to measure immune responsiveness. administration of 50 mg/kg or 100 mg/kg delta-9-thc to b6c3f1 mice in concert with hsv2 infection resulted in suppression of ... | 1987 | 2830329 |
| transactivation of human immunodeficiency virus by herpesviruses. | we examined the interaction of human immunodeficiency virus (hiv) and herpes group viruses. for this purpose, a chimeric plasmid (pltr-cat) was constructed in which the long terminal repeat (ltr) sequences derived from a molecular clone of hiv were fused to a bacterial chloramphenicol acetyltransferase gene (cat). transient expression assays in transfected tissue culture cells were used to monitor the activity of the ltr. basal levels of cat activity were measured in hela and human lung fibrobla ... | 1987 | 2830574 |
| [biological effects of coordinated compounds of transitional metals. 7. antiviral activity of 4-methyl-2-aminopyridine palladium chloride against herpes simplex virus type 1 and type 2 in vitro and in vivo]. | 4-methyl-2-amino-pyridine-palladium chloride (map) showed an antiviral activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in a serum-free medium under in vitro conditions. the replication of these viruses on primary rabbit testes cells was completely suppressed by 10(-5) m/l map. in animal tests using abd2-mice the course of hsv-1 and hsv-2 encephalitis was not influenced by map indicated by mean survival time and lethality. | 1987 | 2830739 |
| glycosylation pattern of herpes simplex virus type 2 glycoprotein g from precursor species to the mature form. | the changes in the apparent molecular weight of herpes simplex virus type 2 glycoprotein g (gg2) were studied by using different [3h] mannose labeling time intervals. various size classes of precursors, probably derived from proteolytic cleavage of the translational product, were identified. our experiments provide evidence that only the 74 kd species is the real precursor of the mature 120 kd gg2. the increase in size is due for the most part to the assembly of o-linked oligosaccharides and to ... | 1987 | 2827609 |
| nuclear accumulation of a heat-shock 70-like protein during herpes simplex virus replication. | a monoclonal antibody defines an antigen, p68, related to hsp70, which is located in nuclei of uninfected exponential cells. nuclear p68 is released by dnase but not rnase treatment suggesting an association with dna. lytic productive infection of confluent quiescent bhk 21 cells with herpes simplex virus type-2 causes p68 to accumulate in nuclei. the effect is specific for hsv-2, and does not occur in hsv-1 infected cells. maximum nuclear accumulation of p68 requires virus dna synthesis althoug ... | 1987 | 2827804 |
| use of densitometric analysis for interpreting hsv serologies based on western blot. | western blot assays have been described for detecting antibodies to herpes simplex virus (hsv). a predominance of antibody binding to either the hsv-1 or the hsv-2-containing blot has been reported to indicate infection with hsv-1 or hsv-2, respectively. by densitometry, differential binding of total antibody on hsv-1 versus hsv-2 strips can be expressed as a ratio. to determine the clinical correlation of these ratios, sera from 81 patients with culture-proven oral or genital herpes were tested ... | 1987 | 2828399 |
| typing of herpes simplex virus types 1 and 2 by immunoblotting analysis using polyclonal antisera to herpes simplex virus glycoproteins. | herpes simplex virus (hsv) isolates were differentiated by immunoblotting analysis using a mixture of polyclonal antisera directed against hsv type 1 (hsv-1) and hsv type 2 (hsv-2) glycoprotein fractions (gb/gc of hsv-1 and gc/ge/84-kda protein of hsv-2), since the mixed antisera recognized viral polypeptides with different molecular weights in hsv-1- and hsv-2-infected cells. results of typing by immunoblotting analysis were consistent with those obtained by restriction endonuclease analysis of ... | 1987 | 2828400 |
| differential metabolism of (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) by equine herpesvirus type 1- and herpes simplex virus-infected cells. | thymidine kinase (tk) enzymes encoded by herpes simplex viruses types 1 and 2 (hsv-1, hsv-2), and equine herpesvirus type 1 (ehv-1) catalyze the phosphorylation of thymidine (dthd) and (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu). the replication of hsv-1 is sensitive to bvdu, but the replication of hsv-2 and ehv-1 is not. to investigate the differential sensitivity of the viruses to halogenated vinyldeoxyuridine drugs, the phosphorylation of 125i-labeled (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu ... | 1987 | 2825591 |
| suppression of humoral antibody formation against sheep red blood cells by infections with hsv-2 and the influence of mouse cytomegalovirus. brief report. | hsv-2 infections suppress the antibody response to hsv-1 but do not impair the mouse cytomegalo virus (mcmv) antibody generating system. in contrast, the sheep red blood cell (srbc) igm response is impaired by preinfections with hsv-2 but not with hsv-1. from the time kinetics of this suppression it can be concluded that only a certain submechanism "spills over" to the srbc system. mcmv suppresses the antibody formation induced by hsv-1. | 1987 | 2825620 |
| ribonucleotide reductase induced by varicella zoster virus. characterization, and potentiation of acyclovir by its inhibition. | an enzyme that catalyzes the conversion of cdp to 2'-dcdp in the presence of dithiothreitol (dtt) was detected in ammonium sulfate fractionated-extracts of varicella zoster virus (vzv)-infected cells. this ribonucleotide reductase was antigenically distinguishable from the isofunctional eucaryotic enzyme as well as the ribonucleotide reductases induced by herpes simplex virus types 1 and 2 (hsv-1 and hsv-2). the vzv-induced enzyme was purified to the extent that most of the contaminating enzymes ... | 1987 | 2825724 |
| reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection. | guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ... | 1987 | 2825951 |
| monoclonal antibody to hsv2 protein as an immunodiagnostic marker in cervical cancer. | the present study was designed to evaluate the possible use of monoclonal antibodies (mabs) as diagnostic adjuncts to exfoliative cytology and tissue sections in intraepithelial (cin) and invasive cervical cancer. specimens were collected from 42 patients with various degrees of cin, 15 patients with invasive cancer and two patients with condylomatous changes only. mab h17, that recognizes a herpes simplex virus protein (icp) representing a component of the viral ribonucleotide reductase, staine ... | 1987 | 2826000 |
| simultaneous presence of herpes simplex and human papilloma virus sequences in human genital tumors. | herpes simplex virus (hsv) and human papillomavirus (hpv) sequences were analyzed in tumors of the female lower genital tract, by probing dna from 13 intraepithelial and 30 invasive neoplastic lesions with radiolabelled hpv-16 and hpv-18 dna as well as cloned fragments of hsv-2 dna. careful removal of stromal tissue from the pathological specimens allowed authentic tumor dna to be processed. normal genital tissue obtained from the patients and genital condylomata were included as internal contro ... | 1987 | 2826337 |
| dna binding of a 38,000-dalton herpes-simplex-virus-2-specific protein. | western transfer and immunoenzymatic staining with affinity-purified monospecific antiserum was used to detect a 38-kd protein that bound to native and denatured dna cellulose. this protein has previously been shown to be a delayed early herpes simplex virus type-2 specific protein. | 1987 | 2826362 |
| evaluation of a monoclonal antibody typing system for herpes simplex virus. | this study compared conventional viral isolation (vi) in cell cultures with a commercial product--virgo antigen detection system--for the identification and typing of herpes simplex virus (hsv). the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. a total of 71 coded specime ... | 1987 | 2819800 |
| intertypic recombinants of herpes simplex virus types 1 and 2 infected-cell polypeptide 4. | the wild-type icp4s (infected-cell polypeptide 4) encoded by herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) are functionally interchangeable. in order to test the functional interchangeability of their intramolecular domains, a series of intertypic icp4 genes was constructed and characterized to determine if any of the encoded chimeric proteins were functionally impaired. we generated the recombinants in escherichia coli using cloned icp4 genes and the lambda recombination vectors develope ... | 1987 | 2820127 |
| occupational risk of hiv, hbv and hsv-2 infections in health care personnel caring for aids patients. | we have prospectively followed for 9-12 months, 246 female health care workers (hcws): 102 with high exposure (he), 43 with low exposure (le), and 101 with no exposure (ne) to aids (acquired immunodeficiency syndrome) patients. no hcws have clinical, serologic, or immunologic evidence of hiv (human immunodeficiency virus) infection. no hcws in the he group seroconverted to cytomegalovirus (cmv). one hcw in the he group seroconverted to hepatitis b virus (hbv), another hcw in the he group serocon ... | 1987 | 2820252 |
| limiting dilution analysis of specific in vitro anti-herpes simplex virus antibody production by human lymphocytes. | an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. hsv specific antibody production was detected in culture supernatants of pbmc from hsv seropositive healthy individuals by stimulating with optimal concentrations of hsv antigen without mitogens. to investigate the specificity of the resulting antibody, a limiting dilution analysis was performed a ... | 1987 | 2820632 |
| improved sensitivity of restriction endonuclease analysis of herpes simplex virus type 2 dna with polyacrylamide gradient gel electrophoresis. | polyacrylamide gradient gel electrophoresis was used to resolve fragments of herpes simplex virus type 2 (hsv-2) dna, produced by the restriction endonucleases alu i, bam hi, pst i, and sma i, which cleave the hsv-2 dna into more than 30 fragments each. hsv-2 strains isolated from different individual patients could be easily distinguished from each other by the endonucleases bam hi and sma i. successive virus isolates from a single person, analyzed using alu i and sma i, showed variability of f ... | 1987 | 2821049 |
| immunity to herpes simplex virus type 2. suppression of virus-induced immune responses in ultraviolet b-irradiated mice. | ultraviolet b irradiation (280 to 320 nm) of mice at the site of intradermal infection with herpes simplex virus type 2 increased the severity of the herpes simplex virus type 2 disease and decreased delayed-type hypersensitivity (dth) responses to viral antigen. decrease in dth resulted from the induction of suppressor t cells, as evidenced by the ability of spleen cells from uv-irradiated mice to inhibit dth and proliferative responses after adoptive transfer. lymph node cells from uv-irradiat ... | 1987 | 2821119 |
| replication characteristics and core size of intranuclear herpes simplex virus (hsv-1) in genital skin lesions: electronmicroscopy studies of a biopsy from a female patient. | herpes simplex virus (hsv) type 1 genital infection, leading to ulcerating lesions in a female patient, was studied by electronmicroscopy. infection had probably been recent, through oro-genital contact with a cold sore on the husband's lip. cell-culture typing and serological tests indicated that the patient currently had an hsv-1 secondary infection. aspects studied in a skin biopsy from an ulcerating labium majus were epidermal cell types infected, stages in virus genesis, virus core diameter ... | 1987 | 2821262 |
| clinical isolate of herpes simplex virus type 2 that induces a thymidine kinase with altered substrate specificity. | in vitro and in vivo studies were done on a herpes simplex virus type 2 strain recovered from a patient on acyclovir (acv) which was acv resistant but expressed thymidine (dthd) kinase (ec 2.7.1.21) activity. plaque-purified clones derived from the original clinical sample were heterogeneous with respect to plaque size and drug susceptibility. the heterogeneity of this viral mixture was also evident from varied 125i-labeled 5-iodo-2'-deoxycytidine autoradiographic patterns and from varied expres ... | 1987 | 2821890 |
| in vitro and in vivo antiviral activity of 2'-fluorinated arabinosides of 5-(2-haloalkyl)uracil. | 5-(2-fluoroethyl)-2'-deoxyuridine (fedu), its 2'-fluoroarabinofuranosyl analog (fefau) and the 2'-fluoroarabinofuranosyl analog (cefau) of the potent anti-herpesvirus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro and in vivo. fedu, fefau and cefau proved to be potent and selective anti-herpesvirus agents in vitro. their potency is evident from their low minimum inhibitory concentrations for hsv-1 and h ... | 1987 | 2821896 |
| activation of latent herpes simplex virus type 2 infection in vitro requires a (e)-5-(2-bromovinyl)-2'-deoxyuridine-sensitive gene function. | previous studies have shown that herpes simplex virus (hsv) type 2 (hsv-2) can be maintained in a latent state in a limited number of cells by elevating the incubation temperature after treatment of hsv-infected human fetus lung fibroblast cells with metabolic inhibitors. superinfection with human cytomegalovirus (hcmv) of latently infected cells maintained at the elevated temperature reactivated latent virus. in addition, superinfection with temperature-sensitive mutants indicated that reactiva ... | 1987 | 2822602 |
| specific phosphorylation of 5-ethyl-2'-deoxyuridine by herpes simplex virus-infected cells and incorporation into viral dna. | 5-ethyl-2'-deoxyuridine (edu) is a potent and selective inhibitor of the replication of herpes simplex virus type 1 (hsv-1) and 2 (hsv-2), which is currently being pursued for the topical treatment of hsv-1 and hsv-2 infections in humans. using [4-14c]edu as the radiolabeled analogue of edu, it was ascertained that, at antivirally active doses, edu is phosphorylated to a much greater extent by hsv-infected vero cells than by mock-infected cells. within the hsv-1-infected cells, edu was incorpora ... | 1987 | 2822705 |
| a sensitive enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigens. | a sandwich elisa for the detection of herpes simplex virus (hsv) antigens was developed using sheep anti-hsv f(ab')2 fragments for capture and an indirect antibody system for detection. current detection limits are 0.5 ng protein for hsv1 and 1.5 ng protein for hsv2. this compares to a single hsv1-infected vero-cell in a background of 10(6) non-infected cells or 10 plaque forming units (pfu) of hsv1 in culture supernatants as determined in separate experiments. limiting dilution experiments show ... | 1987 | 2822751 |
| acetylenic nucleosides. 4. 1-beta-d-arabinofuranosyl-5-ethynylcytosine. improved synthesis and evaluation of biochemical and antiviral properties. | 5-ethynyl-1-beta-d-arabinofuranosylcytosine (eac) was prepared from 1-(2,3,5-tri-o-acetyl-beta-d-arabinofuranosyl)cytosine by iodination followed by coupling with (trimethylsilyl)acetylene and deblocking. at 50 microm, eac was found to inhibit the in vitro replication of herpes simplex virus type 1 and type 2 by greater than 99%. eac also showed activity against a strain of hsv-1 resistant to (e)-5-(2-bromovinyl)-2'-deoxyuridine which has an alteration of the virus-induced thymidine kinase (tk). ... | 1987 | 2822932 |
| evidence that neomycin inhibits binding of herpes simplex virus type 1 to the cellular receptor. | the effect of neomycin, a phosphoinositide-binding aminoglycoside, on herpes simplex virus type 1 (hsv-1) infection of bhk cells was studied. we showed earlier that it specifically inhibits hsv-1 production but not hsv-2 production (langeland et al., biochem biophys. res. commun. 141:198-203, 1986). we now show that neomycin had no effect on cellular protein synthesis, as judged by the appearance of 35s-labeled polypeptides separated by polyacrylamide gel electrophoresis. virus-induced polypepti ... | 1987 | 2822948 |
| thymidylate synthase is the principal target enzyme for the cytostatic activity of (e)-5-(2-bromovinyl)-2'-deoxyuridine against murine mammary carcinoma (fm3a) cells transformed with the herpes simplex virus type 1 or type 2 thymidine kinase gene. | murine mammary carcinoma fm3a cells, deficient in cytosol thymidine (dthd) kinase (tk) activity and transformed by the herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) tk gene (designated fm3a tk-/hsv-1 tk+ and fm3a tk-/hsv-2 tk+, respectively) proved extremely sensitive to the cytostatic action of the potent antiherpetic drugs (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu). the fact that fm3a tk-/hsv-2 tk+ cells were 5-fold more sensitive to the ... | 1987 | 2823092 |
| effects on infants of a first episode of genital herpes during pregnancy. | although genital herpes simplex virus (hsv) infections occurring during pregnancy are known to be associated with neonatal and maternal complications, their frequency and contributing risk factors are not well understood. we prospectively followed 29 patients who acquired genital herpes during pregnancy, to evaluate the perinatal effects of the infection. the patients were classified on the basis of clinical or serologic criteria. fifteen patients had a primary first episode of genital hsv type ... | 1987 | 2823137 |
| [(e)-5-(2-bromovinyl)-2'-desoxyuridine--a new nucleoside analog with selective inhibitory action against herpesviruses. studies in cell culture and animal experiments]. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (1; brvudr) inhibits the replication of herpes simplex virus type 1 (hsv-1) and of varicella-zoster virus (vzv) in vitro at concentrations of 0.01 to 0.23 mumol/l, whereas herpes simplex virus type 2 (hsv-2) is influenced only at 5.5 to 27 mumol/l. in comparison to some classical and newly developed antiherpetics, i. e. 5-iodo-2'-desoxyuridine (2; idoxuridine, idu), 9-beta-d-arabinofuranosyladenine (4; vidarabine ara-a), 9-(2-hydroxyethoxymethyl) guanine (5; ... | 1987 | 2823299 |
| study of ribonucleotide reductase in cells infected with six clinical isolates of herpes simplex virus type 2 (hsv-2) with mutations in its larger subunit. | herpes simplex virus type 2 (hsv-2) induces a novel ribonucleotide reductase (rr) composed of two subunits (140 and 38 kda) in infected cells. other investigators have developed a monoclonal antibody, a6, against the 140-kda subunit of rr and have found, in about 1% of the cases, an inability to detect this protein in cells infected with clinical isolates of hsv-2. we therefore investigated whether in such cases the clinical isolates were capable of inducing viral rr activity and whether the lac ... | 1987 | 2823465 |
| critical determinants of antiherpes efficacy of buciclovir and related acyclic guanosine analogs. | buciclovir is an example of an antiherpes, acyclic guanosine analog activated by the viral thymidine kinase and inhibiting viral dna synthesis in infected cells. an investigation of closely related buciclovir-analogs with similar antiherpes activities in cell cultures and similar, or identical, modes of action but with disparate effects in vivo, revealed the following critical determinants of antiherpes efficacy. (1) the accumulation of guanosine analog-triphosphates in infected cells, which is ... | 1987 | 2823702 |
| redistribution of nuclear ribonucleoprotein antigens during herpes simplex virus infection. | infection of human epidermoid carcinoma no. 2 cells with herpes simplex virus type 1 (hsv-1) leads to a reorganization of antigens associated with both the small and heterogeneous nuclear ribonucleoprotein complexes (snrnp and hnrnp). the hnrnp core protein antigens remain associated with the host chromatin, which appears to collapse into internal aggregates and along the nuclear envelope. more striking is the formation of prominent clusters of snrnp antigens (both general and u1 snrnp specific) ... | 1987 | 2824525 |
| herpes simplex virus glycoproteins gc-1 and gc-2 bind to the third component of complement and provide protection against complement-mediated neutralization of viral infectivity. | cells infected with herpes simplex virus type 1 (hsv-1) form rosettes with c3b-coated erythrocytes, whereas cells infected with herpes simplex virus type 2 (hsv-2) or other herpes viruses do not. it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. we show here that solubilized gc-1 binds to ic3-sepharose affinity columns ... | 1987 | 2824652 |
| efficacy of 5-vinyl-1-beta-d-arabinofuranosyluracil (varau) against herpes simplex virus type 2 strains in cell cultures and against experimental herpes encephalitis in mice: comparison with acyclovir and foscarnet. | the sensitivity of different herpes simplex virus type 2 (hsv-2) strains to inhibition by 5-vinyl-1-beta-d-arabinofuranosyluracil (varau) was evaluated in comparison to 9-(2-hydroxyethoxymethyl)guanine (acv; acyclovir) and trisodiumphosphonoformate (na3pfa; foscarnet), using a plaque inhibition assay in primary rabbit testes (prt) cells as well as in human embryonic lung fibroblast (helf) cell cultures. the order of decreasing activity found was acv much greater than varau greater than na3pfa in ... | 1987 | 2855541 |
| conserved antigenic and functional domains on glycoprotein b of herpes simplex virus 1 and bovine herpesvirus 2. brief report. | a panel of monoclonal antibodies to glycoprotein b (gb) of herpes simplex virus 1 (hsv-1) reacted in immune precipitation and in immunofluorescence tests with a glycoprotein 130,000 in apparent molecular weight specified by bovine herpesvirus 2 (bhv-2). two cross reactive antibodies neutralized the heterologous virus. these results suggest that the bhv-2 glycoprotein is the homologue of hsv-1 gb and shares structural and functional domains expressed on the gb gene product. | 1987 | 2444195 |
| the nucleotide sequence of herpes simplex virus type 2 (333) glycoprotein gb2 and analysis of predicted antigenic sites. | the gene coding for glycoprotein b2 (gb2) of herpes simplex virus type 2 (hsv-2) strain 333 was mapped and its nucleotide sequence determined. open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gb1 of hsv type 1. several predicted features of gb2 are consistent with a membrane-bound glycoprotein, i.e., a signal peptide sequence, a hydrophilic extracellular domain containing possible n-linked glycosylation sites, a hydrophobic membrane spa ... | 1987 | 2446730 |
| stress-related immune suppression: health implications. | this study used a year-long prospective design to assess linkages among distress, immunity, and illness. serial blood samples were collected from 40 first-year medical students at the first, third, and fifth examination periods, as well as 1 month before each. there were significant decrements in the production of gamma-interferon by concanavalin a-stimulated lymphocytes obtained at the time of examinations. antibody titers to epstein-barr virus (ebv) increased during examination periods, sugges ... | 1987 | 2837297 |
| complement component c3b binds directly to purified glycoprotein c of herpes simplex virus types 1 and 2. | cells infected with herpes simplex virus type 1 (hsv-1), but not hsv-2, express on their surfaces a receptor for the complement component c3b. receptor activity is markedly enhanced by treatment of the infected cells with neuraminidase. employing a direct binding assay, consisting of purified hsv glycoproteins immobilized on nitrocellulose and iodinated c3b as a probe, we found that c3b binds directly to gc-1, as well as to gc-2, but not to gb or gd from either serotype. c3b binding was enhanced ... | 1987 | 2849025 |
| vaccine potential of a live avirulent herpes simplex virus. | we previously described a herpes simplex intertypic recombinant that is completely and specifically non-neurovirulent following intracranial inoculation. in this report the vaccine potential of this mutant (re6) is analyzed. it is shown that re6 replicates to wild type levels at the peripheral site of inoculation (the mouse footpad) but, unlike its virulent parental strains, does not replicate to detectable levels in lumbosacral ganglia. a single vaccination with re6 is sufficient to completely ... | 1986 | 2854601 |
| antiviral activity of 5'-paa and 5'-pfa phosphate esters of 2'-deoxyuridines. | the 5'-paa and 5'-pfa phosphate esters of 5-bromo-2'-deoxyuridine (budr) were synthesized and their antiherpes virus activity was evaluated in vitro. both compounds showed activity of the same order as the parent nucleoside, budr, against hsv-2 but were 4 to 12 times less potent against hsv-1. the 5'-paa phosphate ester of budr (ro 21-9875) was also active against varicella-zoster virus (vzv) and human cytomegalovirus (hcmv). the 5'-paa phosphate ester of 5-bromovinyl-2'-deoxyuridine (bvdu) was ... | 1986 | 2947889 |
| antimicrobial activity of various immunomodulators: independence from normal levels of circulating monocytes and natural killer cells. | the effects of 89sr treatment on the natural host resistance of cd-1 mice and the enhancement of resistance by immunomodulators to infection with listeria monocytogenes or herpes simplex virus type 2 (hsv-2) were determined. in the cd-1 mouse, single-dose treatment with 89sr caused a profound decrease in the number of circulating monocytes (mo), lymphocytes, and polymorphonuclear leukocytes (pmn) within 1 week. there was also marked functional impairment of the mo inflammatory response, as well ... | 1986 | 2416693 |
| the effect of food dye and other environmental substances on the host defense reaction in mice in relation to virus infection. | environmental substances were examined for their effect on interferon induction and delayed type hypersensitivity (dth) responses in mice. amaranth, safrole, phenacetin and nicotine suppressed the dth response, and suppressed the serum interferon titers induced by virus infection. however, they did not affect the interferon titers which were induced by tilorone, a chemical inducer. the peak of interferon titer was 12 hours after infection with herpes simplex virus type 2 (hsv-2). therefore, amar ... | 1986 | 2432279 |
| enhancement of host resistance against virus infections by mtp-pe, a synthetic lipophilic muramyl peptide--i. increased survival in mice and guinea pigs after single drug administration prior to infection, and the effect of mtp-pe on interferon levels in sera and lungs. | muramyl tripeptide-phosphatidylethanolamine (mtp-pe, cgp 19835) displays prophylactic antiviral activity in mice infected with influenza viruses a and b, parainfluenza 1 virus or herpes simplex type 1 viruses (hsv/1) and in guinea pigs infected with herpes simplex type 2 viruses (hsv/2). mtp-pe is effective when given in a single intranasal dose as early as 1-4 weeks before infection. in the case of hsv/2 infections, prophylactic effectiveness can be demonstrated after a single topical applicati ... | 1986 | 2433236 |
| metabolism and mode of action of (r)-9-(3,4-dihydroxybutyl)guanine in herpes simplex virus-infected vero cells. | the metabolism and mode of action of the anti-herpes compound buciclovir [r)-9-(3,4-dihydroxybutyl)-guanine, bcv) has been studied in herpes simplex virus-infected and uninfected vero cells. in uninfected cells, a low and constant concentration of intracellular bcv was found, while in herpes simplex virus-infected cells, an increasing concentration of bcv phosphates was found due to metabolic trapping. the major phosphorylation product was bcv triphosphate (bcvtp) which was 92% of the total amou ... | 1986 | 2418021 |
| induction of neutralizing antibody against varicella-zoster virus (vzv) by vzv gp3 and cross-reactivity between vzv gp3 and herpes simplex viruses gb. | glycoprotein gp3 (64k) is one of the major proteins specified by varicella-zoster virus (vzv). this glycoprotein was purified on an immunoadsorbent consisting of monoclonal antibody (clone 8) against gp3 linked to protein a-sepharose. rabbits were then immunized with the purified antigen to obtain monospecific antisera against gp3. the monospecific antisera and monoclonal antibody immunoprecipitated polypeptides with the same molecular weights of approximately 64,000 (64k), 106k, and 116k from a ... | 1986 | 2418583 |
| synthesis of dihydrofolate reductase and metabolism of related rna in a methotrexate resistant human cell line infected with herpes simplex virus type 2. | using a human cell line with amplified gene copy for dihydrofolate reductase (dhfr) and permissive for herpes simplex virus type 2 (hsv-2), the effect of hsv-2 on dhfr synthesis and on the steady-state level of total cellular and nuclear dhfr rna was examined. there was a reduction in dhfr synthesis accompanied by a proportional decrease in the levels of dhfr messenger rna (mrna) 1 hr after infection. both effects could be induced by hsv pretreated with ultraviolet light and this early virus ind ... | 1986 | 2418585 |
| herpes simplex virus specifies two subunits of ribonucleotide reductase encoded by 3'-coterminal transcripts. | we have previously described a transcription unit located between map coordinates 0.558 and 0.595 on the herpes simplex virus type 2 strain 333 genome which encodes two mrnas of 5.0 and 1.2 kilobases that share a common 3' terminus, and we have determined the nucleotide sequence of a 38,000-dalton protein specified by the smaller rna (d. a. galloway and m. a. swain, j. virol. 49:724-730, 1984). the entire nucleotide sequence of the 140,000-dalton protein specified by a 3,432-base-pair open readi ... | 1986 | 2419588 |
| human antibody response to herpes simplex virus-specific polypeptides after primary and recurrent infection. | human antibody responses to specific polypeptides of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) were assessed in serial serum specimens from 18 infected patients by immunoblot technology. nine patients had hsv-1 infections (six genital and three oral) and nine had hsv-2 genital infections. antibodies to homologous and heterologous hsv antigens were studied and correlated with total microneutralization and enzyme-linked immunosorbent assay antibodies as well as correlated ... | 1986 | 2422204 |
| human cytotoxic t cell clones directed against herpes simplex virus-infected cells. iv. recognition and activation by cloned glycoproteins gb and gd. | results of studies in mice and clinical observations in man indicate that t cell-mediated immunity is important in resistance to herpes simplex virus (hsv) infections. this study was undertaken to elucidate the viral antigen specificity of human hsv-immune t cells. purified hsv-1 glycoproteins gb-1 and gd-1, cloned and expressed in mammalian cells, were found to stimulate proliferation of, and interleukin 2 (il 2) production by, peripheral blood lymphocytes (pbl) of hsv seropositive individuals, ... | 1986 | 2423598 |
| an analysis of the biological properties of monoclonal antibodies against glycoprotein d of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. | four monoclonal antibodies to glycoprotein d (gd) of herpes simplex virus (hsv) types 1 and 2 neutralized virus in the presence of complement but exhibited diverse activities in its absence. amino acid substitutions that conferred resistance to neutralization by each antibody were identified by deriving the nucleotide sequence of the gd gene from resistant mutants. each antibody selected a substitution from different parts of the molecule and mutants resistant to a single antibody always arose f ... | 1986 | 2423636 |
| acyclovir treatment of disseminated herpes simplex virus type 2 infection in weanling mice: alteration of mortality and pathogenesis. | intranasal inoculation of weanling mice with herpes simplex virus type 2 (hsv-2) provides an experimental infection that closely resembles disseminated and central nervous system hsv infections of human neonates. intraperitoneal treatment with acyclovir (acv) successfully reduced mortality even when therapy was begun as late as 2 days and oral therapy as late as 4 days after viral challenge. treatment with acv beginning on day 1 completely inhibited hsv-2 replication in lung, spleen, kidney, olf ... | 1986 | 2427021 |
| antiviral effect of two aryl-oligopeptides, fr41565 and fr48217. | macrophages from mice pretreated with two chemically synthesized immunostimulating aryl-oligopeptides, fr41565 and fr48217, inhibited the multiplication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in monkey vero cells, and that of vesicular stomatitis virus (vsv) in murine l929 cells. in addition, the aryl-oligopeptides protected mice against a lethal hsv-1 infection. in particular, when treated with fr48217 at 6 mg/kg, all mice survived, whereas all control mice died from the hsv- ... | 1986 | 2427022 |
| localization of discontinuous epitopes of herpes simplex virus glycoprotein d: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with western blotting. | previously, a panel of monoclonal antibodies (mcab) was used to define specific epitopes of herpes simplex virus glycoprotein d (gd) (r. j. eisenberg et al., j. virol. 53:634-644, 1985). three groups of antibodies recognized continuous epitopes; group vii reacted with residues 11 to 19 of the mature protein (residues 36 to 44 of the predicted sequence), group ii reacted with residues 272 to 279, and group v reacted with residues 340 to 356. four additional antibody groups recognized discontinuou ... | 1986 | 2427745 |
| structural features of ribonucleotide reductase. | herpes simplex virus type 1 (hsv-1) encodes a ribonucleotide reductase which comprises two polypeptides with sizes of 136,000 (rr1) and 38,000 mol. wt. (rr2). we have determined the entire dna sequence specifying hsv-1 rr1 and have identified two adjacent open reading frames in varicella-zoster virus (vzv) which have homology to hsv rr1 and rr2; the predicted sizes for the vzv rr1 and rr2 polypeptides are 87,000 and 35,000 mol. wt. respectively. amino acid comparisons with rr1 and rr2 polypeptid ... | 1986 | 2835765 |
| herpes simplex virus gene products involved in the induction of chromosomal aberrations. | the effect of short-term herpes simplex virus type 1 (hsv-1) infection on chromosomes of human diploid fibroblasts was examined. in addition to chromosomal breaks, gaps and pulverization, three kinds of cytogenetic damage (double minutes, polyploidy and endoreduplication) not yet reported following productive infection with hsv or other animal viruses were frequently observed. consistent with previous studies suggesting that the expression of immediate-early and/or early viral gene products is r ... | 1986 | 3033932 |
| viral alkaline nuclease in intranuclear dense bodies induced by herpes simplex infection. | the distribution of the herpes simplex virus alkaline nuclease (hsv dnase) in rabbit fibroblast cells infected with hsv type 1 or 2 (hsv-1 or hsv-2) has been examined with the aid of immunocytochemical techniques using gold particles as markers. hsv dnase was found to be accumulated within infected nuclei as early as 2.5 hr post-infection. labeled antibody to hsv dnase subsequently increased in all nuclei after 7 hr and 17 hr. at all times post-infection the virus induced nuclear dense bodies we ... | 1986 | 3032315 |
| herpes simplex type ii encephalitis in infancy presenting with focal encephalitis. | herpes virus was recovered from a throat swab, nasopharyngeal washings as well as from brain tissue from a six-month-old infant, who presented with fever, left focal seizures and an enhancing right frontal ct-scan lesion. cytopathic effect (cpe) as seen with genital herpetic infection was seen, suggesting hsv-2. immunofluorescent typing of the virus isolate confirmed hsv-2. early igm positivity preceding a cf and sn titer rise was observed. the patient received a course of ara-a and recovered wi ... | 1986 | 3027605 |
| isolation and characterization of expressible cdna clones encoding the m1 and m2 subunits of mouse ribonucleotide reductase. | mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins m1 and m2, which are differentially regulated during the cell cycle. we have isolated expressible cdna clones of both subunits from an okayama-berg cdna library made with mrna from hydroxyurea-resistant, m2 protein-overproducing mouse ta3 cells. expression of m2 protein could be demonstrated by electron paramagnetic resonance spectroscopy after transfection of cos-7 monkey cells with the plasmid. electrophoresis a ... | 1986 | 3025593 |
| efficacy of vaccination with skinner vaccine towards the prevention of herpes simplex virus induced cervical carcinoma in an experimental mouse model. | the incidence of cervical carcinoma, which had been induced by vaginal application of inactivated herpes simplex virus type 2 (hsv-2) with 20% croton oil, was significantly reduced in mice prevaccinated with the skinner herpes vaccine. there was evidence of an immunological response in both vaccinated and unvaccinated mice. | 1986 | 3026107 |
| [immunohistological study on malignant and premalignant lesions of the uterine cervix associated with herpes simplex virus]. | herpes simplex virus 2 (hsv-2) has become the object of public attention as an etiological cause of cervical cancer. uneven distribution of hsv antigen in tissue was examined dy dyeing tissue materials of cervical intraepithelial neoplasia by the method called peroxidase-antiperoxidase using hsv-2 antibody. the positive rate in the control group was 10.3%, while it was 10.7% in the mild dysplasia group, 17.5% in the moderate dysplasia group, 25.5% in the severe dysplasia group, 31.3% in the carc ... | 1986 | 3025314 |
| purulent penile ulcers of patients in singapore. | in 80 patients with painful purulent penile ulcers who attended the outpatient service of middle road hospital in singapore, haemophilus ducreyi was isolated from 18 (22%), herpes simplex virus type 2 from nine (11%), and neisseria gonorrhoeae from eight (10%). primary pathogens were not isolated from 45 (57%) men. painful purulent penile ulcers were more common in uncircumcised men, and patients had often acquired the disease after sexual intercourse with prostitutes in singapore. | 1986 | 3015775 |
| prospective study on the relationship between cervical neoplasia and herpes simplex type 2 virus. iii. presence of herpes simplex type-2 antibody in sera of subjects who developed cervical neoplasia later in the study. | paired sera taken from 63 subjects who developed cervical neoplasia in the course of a prospective study on this disease were examined for the presence of herpes simplex virus type 2 (hsv-2) antibody. the first serum was taken at enrollment into the study, while the second was obtained after development of the disease, i.e. 2-4 years later. simultaneously, paired sera from a group of control subjects, who remained free of any cytological and colposcopical abnormalities throughout the study, were ... | 1986 | 3015805 |
| antibody response, recurrence patterns and subsequent herpes simplex virus type 2 (hsv-2) re-infection following initial hsv-2 infection of guinea-pigs: effects of acyclovir. | the production of antibody to specific herpes simplex virus type 2 (hsv-2) polypeptides, the recurrence patterns and the susceptibility to re-infection were studied in the guinea-pig model of genital hsv-2 infection. further, we defined the effects of acyclovir (acv) therapy on these parameters of infection. treatment with acv reduced the clinical severity of the initial disease but did not affect vaginal viral shedding. production of neutralizing antibody as well as antibody to the nucleocapsid ... | 1986 | 3016155 |
| asymptomatic virus shedding in men with genital herpes infection. | 13 men with a history of recurrent genital herpes simplex virus type 2 (hsv-2) infection were followed daily for 4 weeks with samples taken from the urethra for virus isolation. asymptomatic virus shedding occurred in 5 men who had 1 single positive isolation each. four of these urethral isolates were typed as hsv-1 and 1 as hsv-2. | 1986 | 3016883 |
| a rapid detection of herpes simplex virus by elisa in asymptomatic pregnant women and neonates. | herpes simplex virus (hsv) was detected by the enzyme-linked immunosorbent assay (elisa). the assay system employed rabbit anti-hsv-2-coated microplates to detect hsv in clinical specimens and the same reagent labelled with peroxidase as a conjugate. the hsv type 2 obtained from vero cell culture and normal cell lysate (ncl) were used as positive and negative reference antigens respectively. hsv was detected in 40 (9.93%) of vaginal swabs obtained from 403 pregnant women just before the deliveri ... | 1986 | 3016912 |
| the herpes simplex virus type 2 equivalent of the herpes simplex virus type 1 us7 gene and its flanking sequences. | nucleotide sequencing studies (d. j. mcgeoch, a. dolan, s. donald, and f. rixon, 1985, j. mol. biol. 181, 1-14) have indicated that herpes simplex virus type 1 (hsv-1) has a coding sequence, referred to as us7, between the genes for the glycoproteins d and e (gd and ge). northern blot analysis and nucleotide sequencing have been carried out to show that the type 2 virus (hsv-2) has an equivalent to the us7 gene. a comparison with the hsv-1 sequence has revealed some surprising similarities and d ... | 1986 | 3016980 |
| herpes-related polypeptides from a human cervical carcinoma cell line. | using antiserum against herpes simplex virus type 2 (hsv-2) infected cells, eight polypeptides with similar molecular weights could be immunoprecipitated from the nearly diploid, human cervical carcinoma cell line c4ii and from hsv-2 transformed hamster and mouse cells. only two of these polypeptides corresponded to those from hsv-2 infected cells, including the putative hsv-2 transformation-related 35k protein. partial proteolytic cleavage products of the immunoprecipitated 35k polypeptides fro ... | 1986 | 3017554 |
| cryosurvival of herpes simplex virus-2 during cryopreservation of human spermatozoa. | herpes simplex virus (hsv) is a sexually transmitted agent which has potential association with cervical cancer, as well as with high morbidity and mortality in perinatal infection. its incidence is estimated just after gonorrhea and chlamydia trachomatis infections. cryobanking of human semen is accepted worldwide in therapeutic use for both husband and donor. serious consequences could follow the clinical applications of cryobanked human semen in insemination, if some ejaculates contain hsv an ... | 1986 | 3017635 |
| failure to induce cervical cancer in mice by long-term frequent vaginal exposure to live or inactivated herpes simplex viruses. | c57 mice aged 8-10 weeks in groups of 50 each received vaginal cotton pellets soaked in lysates of hep-2 cells, either mock-infected or infected with herpes simplex virus i, herpes simplex virus 2, and highly attenuated recombinant viruses 5 times a week for 89 to 114 weeks. an untreated group was also included. the mock-infected and some of the infected cell lysates were exposed to ultraviolet light at a dose sufficient to inactivate virus. smears of exfoliated vaginal cells collected once a mo ... | 1986 | 3017867 |
| analyses of transplanted murine tumors for hsv dna sequences. | meignier et al. (1986) report the results of exposure of c57bl/6ncr mice to vaginal plugs containing live or inactivated herpes simplex virus 1 or 2 (hsv-1 or hsv-2) or recombinant viruses 5 times a week for up to 114 weeks. genital organs showing abnormalities were transplanted into nude mice. of 33 transplants, 13 produced subcutaneous tumors in nude mice and 12 were subsequently transplanted into c57bl/6ncr mice. we report that the dna extracted from coded tumor tissues of nude mice and from ... | 1986 | 3017868 |
| zosteriform spread of herpes simplex virus type 2 genital infection in the guinea-pig. | a model of herpes simplex virus type 2 (hsv-2) infection was developed in the guinea-pig that permits investigation of the role of neural spread of virus in the pathogenesis of genital skin disease. after hsv-2 inoculation of an abraded area lateral to the external genital skin, virus was first detected in the ipsilateral (to the inoculation site) peripheral nerves or genital skin on day 2 or 3 post-inoculation followed by detection in the ipsilateral dorsal root ganglia and spinal cord on day 3 ... | 1986 | 3018127 |
| genetically determined difference in the antiviral action of alpha/beta interferon in cells from mice resistant or susceptible to herpes simplex virus type 2. | resistance of mice to infection with herpes simplex virus type 2 (hsv-2) is genetically determined. embryonic cells from susceptible balb/c and resistant c57bl/6 mice were equally sensitive to infection with hsv-2 as judged by plaque area, plaquing efficiency, endpoint titration and virus yield. cells from c57bl/6 mice showed a higher sensitivity than cells from balb/c mice to the protective action of two preparations of alpha/beta interferon against challenge with hsv-2. this was evident both f ... | 1986 | 3018128 |
| delta-9-tetrahydrocannabinol enhances release of herpes simplex virus type 2. | this study was undertaken to determine the effect of micromolar concentrations of delta-9-tetrahydrocannabinol (delta-9-thc) on herpes simplex virus type 2 (hsv-2) replication in vitro. virus-infected vero cells pretreated for 24 h with 10(-5) m- or 10(-6) m-delta-9-thc yielded 100-fold increases in infectious extracellular virus. transmission electron microscopy of drug-treated cells revealed plasma membrane dissolution, distension of the smooth and rough endoplasmic reticulum, and the appearan ... | 1986 | 3018134 |
| carbocyclic analogues of 5-halocytosine nucleosides. | carbocyclic analogues of 5-halocytosine nucleosides were prepared by direct halogenation of the carbocyclic analogues of cytidine, 2'-deoxycytidine, 3'-deoxycytidine, or ara-c. the 5-chloro and 5-bromo derivatives of the cytidine (carbodine) and of the 2'-deoxycytidine analogues and the 5-iodo derivatives of all four of the cytosine nucleoside analogues were prepared. all of the c-5-halocytosine nucleosides, as well as the parent c-cytosine nucleosides, were tested against a strain of herpes sim ... | 1986 | 3018246 |
| herpes simplex virus suppression of human endothelial matrix protein synthesis is independent of viral protein synthesis. | protein synthesis was determined in cultures of human umbilical cord vein endothelial cells (ec) infected with either herpes simplex type 1 (hsv-1) or type 2 (hsv-2). monolayers were infected for 1 hour with either 5 or 20 infectious virus particles per ec (multiplicity of infection of 5 or 20). at different times after infection, infected and noninfected cultures were pulsed with either 5 mu ci/ml of [14c]proline or 25 mu ci/ml of [35s]methionine for 1 or 2 hours. autoradiograms of sodium dodec ... | 1986 | 3018360 |
| synergistic inhibitory effect of acyclovir and human native beta-interferon on the growth of herpes simplex virus type 2 in human embryo fibroblast cell cultures. | 1986 | 3018459 | |
| antiherpetic effects of a human alpha interferon analog, ifn-alpha con1, in hamsters. | the efficacy of a novel consensus form of human alpha interferon designated ifn-alpha con1 was evaluated against herpesvirus infections in vitro and in vivo. at comparable antiviral concentrations, natural lymphoblastoid ifn, ifn-alpha con1, the molecular subtype ifn-alpha, and the hybrid ifn-alpha ad(bgl) obtained by recombinant dna methods conferred similar protection against herpes simplex virus type 1 and type 2 (hsv-2) infections of human cells in vitro. whereas 7 x 10(5) u of ifn-alpha ad( ... | 1986 | 3019238 |
| specific transfer factor protects mice against lethal challenge with herpes simplex virus. | bovine transfer factor (tfd) specific to herpes simplex virus (hsv)1 or to hsv2 was prepared by immunizing calves with the corresponding virus. the tfd preparations were then injected into swiss mice in an attempt to protect them against a subsequent lethal challenge with hsv1 or hsv2 virus. it was thus shown that injection of anti-hsv tfd protects the mice against the corresponding hsv virus, whereas the injection of a nonspecific tfd (anti-cmv) fails to protect against a challenge with hsv1. f ... | 1986 | 3019568 |
| human papillomavirus in clinically and histologically normal tissue of patients with genital cancer. | to study the association of human papillomavirus (hpv) and herpes simplex virus (hsv) with genital cancer, we collected specimens of cervical, vulvar, endometrial, and vaginal tumors at the time of operation in patients with cancer. in some patients, matched internal-control (histologically normal) tissue was also collected. dna extracted from the tissue was probed with radiolabeled hpv type 16 dna, hpv type 18 dna, and cloned fragments of hsv type 2 dna. hybridization to the hindiiia clone of h ... | 1986 | 3020404 |
| localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements. | the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ... | 1986 | 3020562 |
| lack of evidence for intertypic recombinants in the pathogenesis of recurrent genital infections with herpes simplex virus type 1. | clinical observations indicate that herpes simplex virus type 1 (hsv-1) is significantly less likely than herpes simplex virus type 2 (hsv-2) to establish latency in (or reactivate from) sacral ganglionic tissue. in an effort to identify viral functions associated with latency, we analyzed hsv-1 isolates from three patients with established recurrent genital herpes and sought evidence of dna sequences and proteins similar to those found in hsv-2. by restriction endonuclease cleavage patterns and ... | 1986 | 3020720 |
| passage of herpes simplex virus type 1 on chick embryo fibroblasts confers virulence for chick embryos. | the pathogenesis of chorioallantoic membrane (cam) infection with herpes simplex virus 1 and 2 (hsv-1 and hsv-2) as well as chick embryo fibroblast (cef) passaged hsv-1 was studied. it was found that hsv-2 is at least a million-fold more virulent than hsv-1 as measured by pfu/ld50 ratios for the embryo. serial passage of hsv-1 in vitro on cef cells selected for a virus (cefp10) which, unlike its parental hsv-1 strain, is able to kill the embryo. the restriction endonuclease maps of cefp10 and it ... | 1986 | 3020830 |
| in vitro activity of ketoconazole against herpes simplex virus. | the effects of ketoconazole alone and in combination with acyclovir and adenine arabinoside upon the replication of herpes simplex virus types 1 and 2 (hsv-1 and -2) were investigated by using a yield reduction assay. ketoconazole demonstrated antiviral activity against hsv-1 and -2 and synergistic antiviral activity when it was combined with acyclovir. combinations of ketoconazole with adenine arabinoside resulted in either interference or indifference. the effects of ketoconazole upon the prot ... | 1986 | 3021048 |
| establishment of mutant murine mammary carcinoma fm3a cell strains transformed with the herpes simplex virus type 2 thymidine kinase gene. | to establish cell systems appropriate for investigating the mode of action of antiherpetic nucleoside analogues, mutant cell strains were constructed from murine mammary carcinoma fm3a cells, which were deficient in tk, but were transformed with a recombinant plasmid dna containing the hsv-2 tk gene. the transformed cells incorporated the viral dna, expressed viral tk activity and showed unusually high sensitivity to the cytostatic action of the antiherpetic nucleoside analogues acv and ivdu, bo ... | 1986 | 3021345 |