Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a mutant of salmonella enterica serovar typhimurium rna polymerase extracytoplasmic stress response sigma factor sigma(e) with altered promoter specificity. | the alternative sigma factor sigma(e) is critical for envelope stress response and plays a role in pathogenicity of a variety of different bacteria. we previously identified several critical nucleotides in the salmonella enterica serovar typhimurium (s. typhimurium) sigma(e)-dependent rpoep3 promoter that corresponded to the most conserved nucleotides in the sigma(e) consensus sequence of the -10 and -35 promoter elements. in the present study, we exploited a previously established escherichia c ... | 2009 | 19415331 |
| comparison of genetic and physiological properties of salmonella enterica isolates from chickens reveals one major difference between serovar kentucky and other serovars: response to acid. | for unknown reasons, salmonella enterica kentucky has become the serovar most frequently isolated from chickens and chicken carcasses in the united states. in an attempt to identify traits that may underlie this phenomenon, genetic and physiological features of 30 serovar kentucky chicken isolates were compared with those of chicken isolates belonging to a range of other s. enterica serovars. most of the well-known salmonella virulence genes were detected in the serovar kentucky isolates by pcr, ... | 2009 | 19415975 |
| salmonella enterica serovar typhi live vector vaccines finally come of age. | attenuated salmonella typhi vaccine strains hold great promise as live vectors for presentation of foreign antigens from unrelated bacterial, viral and parasitic pathogens to the immune system. although this approach has proved quite successful in experimental animal models for eliciting antigen-specific mucosal, humoral and cellular responses, results have been disappointing for clinical trials carried out thus far. we hypothesize that the paucity of human responses to foreign antigens delivere ... | 2009 | 19417771 |
| the structure of a cytolytic alpha-helical toxin pore reveals its assembly mechanism. | pore-forming toxins (pfts) are a class of potent virulence factors that convert from a soluble form to a membrane-integrated pore. they exhibit their toxic effect either by destruction of the membrane permeability barrier or by delivery of toxic components through the pores. among the group of bacterial pfts are some of the most dangerous toxins, such as diphtheria and anthrax toxin. examples of eukaryotic pfts are perforin and the membrane-attack complex, proteins of the immune system. pfts can ... | 2009 | 19421192 |
| effects of static magnetic field on cell growth, viability, and differential gene expression in salmonella. | in the present study, we investigated the effect of exposure to a static magnetic field (smf) on cell growth, viability, and gene expression of salmonella enterica subsp. enterica serovar hadar. our results indicated that smf exposure (200 mt, 13 hours) failed to alter cellular growth but induced a decrease of colony-forming units (cfu) between 3 and 6 hours followed by an increase from 6 to 9 hours. the analysis of the differential expression of rpoa, dnak, katn, and 16s rrna genes under smf ex ... | 2009 | 19422305 |
| analysis of antimicrobial resistance and plasmid profiles in salmonella serovars associated with tropical seafood of india. | a total of 256 salmonella strains consisting of 29 salmonella serovars isolated from seafood of cochin (india) were analyzed for resistance to antimicrobials commonly used in human and veterinary medicines as therapeutic agents. the 10 most predominant salmonella serovars in seafood were also characterized for presence of plasmids using the alkaline lysis method. antimicrobial susceptibility studies highlighted a comparatively high resistance in salmonella isolates to sulfamethizol and carbenici ... | 2009 | 19422307 |
| serotype distribution and antibiotic resistance of human gastrointestinal isolates of salmonella enterica from crete, greece. | 2009 | 19423479 | |
| conjugative transfer of plasmid-located antibiotic resistance genes within the gastrointestinal tract of lesser mealworm larvae, alphitobius diaperinus (coleoptera: tenebrionidae). | the frequency of conjugative transfer of antimicrobial resistance plasmids between bacteria within the gastrointestinal tract of lesser mealworm larvae, a prevalent pest in poultry production facilities, was determined. lesser mealworm larvae were exposed to a negative bacterial control, a donor salmonella enterica serotype newport strain, a recipient escherichia coli, or both donor and recipient to examine horizontal gene transfer of plasmids. horizontal gene transfer was validated post externa ... | 2009 | 19425825 |
| sanitation of faeces from source-separating dry toilets using urea. | to develop a reliable and simple method to produce safe fertilizers from human excreta using urea for sanitation of faeces. | 2009 | 19426280 |
| identifying promoter features of co-regulated genes with similar network motifs. | a large amount of computational and experimental work has been devoted to uncovering network motifs in gene regulatory networks. the leading hypothesis is that evolutionary processes independently selected recurrent architectural relationships among regulators and target genes (motifs) to produce characteristic expression patterns of its members. however, even with the same architecture, the genes may still be differentially expressed. therefore, to define fully the expression of a group of gene ... | 2009 | 19426448 |
| effect of expression level on immune responses to recombinant oral salmonella enterica serovar typhimurium vaccines. | live, attenuated salmonella has been used to express heterologous antigens for development of oral vaccines. often, expression must be regulated because of deleterious effects on the salmonella vector. the effect of varying expression levels on immune response parameters has not been well defined. in this study we introduced mutations in the -10 region of the trc promoter in the expression plasmid to generate series of vaccine strains with different levels of expression of a model antigen, the h ... | 2009 | 19428883 |
| sopb of salmonella enterica serovar typhimurium is a potential dna vaccine candidate in conjugation with live attenuated bacteria. | the immune response against salmonella is multi-faceted involving both the innate and the adaptive immune system. the characterization of specific salmonella antigens inducing immune response could critically contribute to the development of epitope based vaccines for salmonella. we have tried to identify a protective t cell epitope(s) of salmonella, as cell mediated immunity conferred by cd8+ t cells is the most crucial subset conferring protective immunity against salmonella. it being a proven ... | 2009 | 19428891 |
| potential role for the 4,12 antigen in the prevalence of clonal salmonella serovars in poultry. | 2009 | 19429568 | |
| isolation of salmonella typhimurium from dead blue and gold macaws (ara ararauna). | two blue and gold macaw (ara ararauna) chicks died of fatal salmonellosis in buenos aires province, argentina. the birds were histopathologically and microbiologically examined. salmonella enterica subspecies enterica serovar typhimurium was isolated from the liver, spleen, heart, lung, kidney, and intestine of both birds. all strains were susceptible to ampicillin, cephalothin, cefotaxime, enrofloxacin, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, tetracycline, nitrofu ... | 2009 | 19432017 |
| salmonella enterica outbreak in a banqueting hall in jerusalem: the unseen hand of the epidemiological triangle? | foodborne salmonella enterica outbreaks constitute both a threat to public health and an economic burden worldwide. | 2009 | 19432037 |
| drug-resistant salmonella typhimurium dt 120: use of pfge and mlva in a putative international outbreak investigation. | isolates of salmonella enterica serovar typhimurium belonging to definitive phage type (dt) 120 (salmonella typhimurium dt 120) from simultaneous outbreaks of infection in the england and denmark have been compared on the basis of antibiogram, pulsed-field gel electrophoresis (pfge), and multiple locus variable number tandem repeat analysis (mlva). isolates from england had the resistance profile (ampicillin, streptomycin, sulfamethoxazole, and tetracycline), mlva profiles 2-4-4-0-2, 2-4-5-0-2, ... | 2009 | 19432517 |
| perturbation of the small intestine microbial ecology by streptomycin alters pathology in a salmonella enterica serovar typhimurium murine model of infection. | the small intestine is an important site of infection for many enteric bacterial pathogens, and murine models, including the streptomycin-treated mouse model of infection, are frequently used to study these infections. the environment of the mouse small intestine and the microbiota with which enteric pathogens are likely to interact, however, have not been well described. therefore, we compared the microbiota and the concentrations of short-chain fatty acids (scfas) present in the ileum and cecu ... | 2009 | 19433544 |
| internalization of salmonella enterica serovar montevideo into greenhouse tomato plants through contaminated irrigation water or seed stock. | tomatoes have been linked to outbreaks of salmonellosis, demonstrating the need to identify sources of contamination. objectives of this study included determining the ability for salmonella enterica serovar montevideo to be internalized into tomatoes from contaminated irrigation water and seed stock, and establishing whether salmonella montevideo can survive in fertilizer solutions. six treatment groups (five plants per group) were irrigated with 350 ml of 7 log cfu/ml of salmonella montevideo ... | 2009 | 19435236 |
| characterization of mgtc, a virulence factor of salmonella enterica serovar typhi. | the mgtc is a virulence factor in salmonella typhimurium that is required for growth at low-mg2+ concentrations and intramacrophage survival. this gene is codified in a conserved region of the salmonella pathogenicity island 3 (spi-3), and is also present in the chromosome of other salmonella serovars. in this study we characterized the mgtc factor in s. typhi, a human specific pathogen, by using mgtc and spi-3 mutant strains. we found that mgtc is the most important factor codified in the spi-3 ... | 2009 | 19436747 |
| scamp3 is a component of the salmonella-induced tubular network and reveals an interaction between bacterial effectors and post-golgi trafficking. | salmonella enterica are facultative intracellular bacterial pathogens that proliferate within host cells in a membrane-bounded compartment, the salmonella-containing vacuole (scv). intracellular replication of salmonella is mediated by bacterial effectors translocated on to the cytoplasmic face of the scv membrane by a type iii secretion system. some of these effectors manipulate the host endocytic pathway, resulting in the formation in epithelial cells of tubules enriched in late endosomal mark ... | 2009 | 19438519 |
| [characterization of attenuated salmonella c500 strain with a delta asd mutant and use as an asd+ balanced-lethal host-vector system]. | salmonella enterica serovar choleraesuis strain c500 is a live, attenuated vaccine that has been used in china for over 40 years to prevent piglet paratyphoid. the objective of this study was to evaluate the potential of attenuated salmonella enterica serovar choleraesuis c500 strain with a delta asd mutant as an effective live vaccine vector by the asd+ balanced-lethal host-vector system. here, we compared the characteristics of s. enterica serovar choleraesuis delta asdc500 strain with the par ... | 2009 | 19441223 |
| detection of salmonella enterica serovar typhimurium with puo-stvr2-like virulence-resistance hybrid plasmids in the united kingdom. | the aim of this study was to investigate the presence in the united kingdom (uk) of salmonella enterica serovar typhimurium isolates carrying puo-stvr2-like virulence-resistance hybrid plasmids that originated from pslt. one hundred and fifty ampicillin-resistant isolates of s. typhimurium, collected in different regions of the uk during 2006, were screened for the presence of bla (oxa-1) carried by an inh-like integron (2000 bp/bla (oxa-1)-aada1) characteristic of puo-stvr2. positive isolates w ... | 2009 | 19444492 |
| non-typhoidal salmonella bacteraemia: epidemiology, clinical characteristics and its' association with severe immunosuppression. | non-typhoidal salmonella (nts) is increasingly recognized as an important pathogen associated with bacteraemia especially in immunosuppressed patients. however, there is limited data specifically describing the clinical characteristics and outcome amongst the immunosuppressed patients. | 2009 | 19445730 |
| salmonella typhimurium: insight into the multi-faceted role of the lysr-type transcriptional regulators in salmonella. | the lysr-type transcriptional regulators (lttrs) are widely distributed in various genera of prokaryotes. lttrs are dna binding proteins that can positively or negatively regulate target gene expression and can also repress their own transcription. salmonella enterica comprises a group of gram-negative bacteria capable of causing clinical syndromes that range from self-limiting diarrhoea to severe fibrinopurulent necrotizing enteritis and life threatening systemic disease. the survival and repli ... | 2009 | 19447191 |
| redundant hydrogen peroxide scavengers contribute to salmonella virulence and oxidative stress resistance. | salmonella enterica serovar typhimurium is an intracellular pathogen that can survive and replicate within macrophages. one of the host defense mechanisms that salmonella encounters during infection is the production of reactive oxygen species by the phagocyte nadph oxidase. among them, hydrogen peroxide (h(2)o(2)) can diffuse across bacterial membranes and damage biomolecules. genome analysis allowed us to identify five genes encoding h(2)o(2) degrading enzymes: three catalases (kate, katg, and ... | 2009 | 19447905 |
| rapid detection of salmonella in milk by combined immunomagnetic separation-polymerase chain reaction assay. | during the past few years, milk has presented a risk of salmonella contamination; it has been implicated as the cause in several outbreaks of salmonellosis. because conventional detection methods require 5 to 7 d for completion and involve several subcultivation stages followed by biochemical and serological tests, rapid and sensitive methods have been sought, mainly at the dna level. therefore, a study including milk samples was conducted to evaluate the performance of a combination of 2 techni ... | 2009 | 19447970 |
| effect of analgesics and their derivatives on antibiotic resistance of environmental microbes. | this research is a preliminary study conducted to determine the effects of aspirin (acetyl-salicylic acid) and salicylic acid (analgesics and their derivatives) on the antibiotic resistance of ammonia oxidizing bacterium (aob) (a non-pathogenic environmental microbe) cultured from the texas tech university-water recovery system that treats a space related wastewater for nasa. the effect of salicylic acid was investigated by obtaining the minimal inhibition concentration (mic) of antibiotics (amo ... | 2009 | 19448319 |
| salmonella enterica serovar typhimurium-induced internalization and il-8 expression in hela cells does not have a direct relationship with intracellular ca(2+) levels. | the invasion-associated type iii secretion system (t3ss-1) of s. typhimurium is required to initiate and sustain an acute inflammatory response in the intestine. we investigated the relationship of s. typhimurium t3ss-1-induced il-8 expression and invasion with intracellular ca(2+) mobilization in hela cells. compared to the sipasopabde2 mutant, strains carrying a mutation in sipa, or mutations in sopabde2 induced higher levels of il-8 and greater bacterial internalization despite the fact that ... | 2009 | 19450704 |
| the capsule-encoding viab locus reduces intestinal inflammation by a salmonella pathogenicity island 1-independent mechanism. | salmonella enterica serotype typhimurium elicits acute neutrophil influx in the human intestinal mucosa within 1 or 2 days after infection, resulting in inflammatory diarrhea. in contrast, no overt symptoms are observed within the first 1 or 2 weeks after infection with s. enterica serotype typhi. here we show that introduction of the capsule-encoding viab locus of serotype typhi reduced the ability of serotype typhimurium to elicit acute intestinal inflammation in a streptomycin-pretreated mous ... | 2009 | 19451244 |
| first report of plasmid-mediated quinolone resistance determinant qnrs1 in an escherichia coli strain of animal origin in italy. | a qnrs1-positive strain of escherichia coli was detected among 73 poultry isolates showing ciprofloxacin mics of > or =0.125 microg/ml. the qnrs1 gene was associated with a tn3-like transposon, as previously described to occur in a salmonella enterica serovar infantis strain of animal origin, but the plasmid scaffold carrying this element resembled that of a plasmid previously identified in salmonella enterica serovar dublin. these elements suggest genetic exchanges among salmonella and e. coli ... | 2009 | 19451298 |
| nitric oxide-induced membrane tubulovesicular extensions (cytonemes) of human neutrophils catch and hold salmonella enterica serovar typhimurium at a distance from the cell surface. | nitric oxide (no) plays an important role in host defense against bacterial infections such as salmonellosis. no and 4-bromophenacyl bromide (bpb) induce the formation of long tubulovesicular extensions (tve, cytonemes, membrane tethers) from human neutrophils. these tve serve as cellular sensory and adhesive organelles. in the present study, we demonstrated that in the presence of the no donor, diethylamine nonoate or bpb human neutrophils bound and aggregated salmonella enterica serovar typhim ... | 2009 | 19453754 |
| host-specificity of salmonella enterica serovar gallinarum: insights from comparative genomics. | in this study, we have identified the possible genetic factors responsible for fowl-adaptation of salmonella entericaserovar gallinarum (s. gallinarum). by comparing the genes related to salmonella pathogenicity islands (spi) of s. gallinarum with those of salmonella entericaserovar enteritidis (s. enteritidis) we have identified twenty-four positively selected genes. our results suggest that the genes encoding the structural components of spi-2 encoded type three secretion apparatus (ttss) and ... | 2009 | 19454277 |
| a precious metal heist. | nearly all bacterial pathogens require iron to successfully infect their vertebrate hosts. the host molecule lipocalin-2 exploits this by sequestering bacterial siderophores as a mechanism of protection against infection. raffatellu et al. (2009) show that salmonella enterica serotype typhimurium circumvents this through the production of the modified siderophore salmochelin. | 2009 | 19454345 |
| lipocalin-2 resistance confers an advantage to salmonella enterica serotype typhimurium for growth and survival in the inflamed intestine. | in response to enteric pathogens, the inflamed intestine produces antimicrobial proteins, a process mediated by the cytokines il-17 and il-22. salmonella enterica serotype typhimurium thrives in the inflamed intestinal environment, suggesting that the pathogen is resistant to antimicrobials it encounters in the intestinal lumen. however, the identity of these antimicrobials and corresponding bacterial resistance mechanisms remain unknown. here, we report that enteric infection of rhesus macaques ... | 2009 | 19454351 |
| survival of enteric bacteria and coliphage ms2 in pure human urine. | the survival of escherichia coli, salmonella enterica serovar typhimurium, enterococcus faecalis and coliphage ms2 was studied in stored, fresh and diluted (1 : 1) human urine at 15 and 30 degrees c. | 2009 | 19457041 |
| antimicrobial activity of enterococcus faecium ef 55 against salmonella enteritidis in chicks. | the protective effect of enterococcus faecium ef 55 against salmonella enterica serovar enteritidis phage type 4 (se pt4) was studied in 1-day-old chicks. the ef 55 strain (isolated and characterised by the authors earlier) was applied daily (1.10(9) cfu/0.2 ml pbs) for 7 days. oral inoculation of the se pt4 strain was performed on day 8 in a single dose of 5.10(8) cfu/0.2 ml pbs. the experiment lasted for 21 days. samples were collected on day 1 of the experiment to verify the absence of salmon ... | 2009 | 19457770 |
| a yeast-based genetic screen for identification of pathogenic salmonella proteins. | salmonella uses type iii secretion systems (ttss) to deliver pathogenic proteins into the host cells. these translocated effectors induce bacterial internalization and intracellular proliferation by targeting important cellular processes that are conserved among eukaryotes. here, we assessed the feasibility of performing a genetic screen in yeast to identify novel salmonella effectors, by searching for genes that produce toxicity when expressed in this model system. we identified several known t ... | 2009 | 19459960 |
| molecular typing methodologies for microbial source tracking and epidemiological investigations of gram-negative bacterial foodborne pathogens. | gram-negative bacterial foodborne pathogens are a worldwide cause of morbidity and mortality. the ability to carry out epidemiological investigations to determine the primary sources of bacterial contamination is important to improve public health. multiple methods are available for bacterial source tracking and to determine the distribution of pathogens isolated from sick patients. the molecular based typing methods available fall into three general categories: those based on restriction analys ... | 2009 | 19460308 |
| soxs regulates the expression of the salmonella enterica serovar typhimurium ompw gene. | ompw of salmonella enterica serovar typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. biochemical and genetic evidence from our laboratory indicates that ompw is involved in efflux of and resistance towards paraquat (pq), and its expression has been shown to be activated in response to oxidative stress. in this study we have explored ompw expression in response to pq. primer extension and transcriptional fusions showed t ... | 2009 | 19460824 |
| structure of o-antigen and functional characterization of o-antigen gene cluster of salmonella enterica o47 containing ribitol phosphate and 2-acetimidoylamino-2,6-dideoxy-l-galactose. | an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of salmonella enterica o47 and studied by sugar analysis along with one- and two-dimensional 1h- and 13c-nmr spectroscopy. the following structure of the linear ribitol phosphate-containing repeating unit of the o-polysaccharide was established: -->2)-d-ribitol-5-p-(o-->6)-alpha-d-galp-(1-->3)-alpha-l-fucpnam-(1-->3)-beta-d-glcpnac-(1-->, where fucnam stands for 2-acetimidoylamino-2,6-dideoxy-l-galactose. about 1 ... | 2009 | 19463095 |
| flow cytometric analysis of salmonella enterica serotype typhimurium inactivated with supercritical carbon dioxide. | non-thermal processes for the effective sterilization and inactivation of microorganisms are currently receiving a great deal of attention in food, pharmaceutical and other relevant industries. supercritical carbon dioxide (sc-co(2)) treatment is an alternative method of microbial inactivation that can be safely used in foods and bioactive materials at relatively low temperatures. however, to date, the inactivation of microbial cells by treatment with sc-co(2) has only been evaluated using a con ... | 2009 | 19463861 |
| evaluation of salmonella live vaccines with chromosomal expression cassettes for translocated fusion proteins. | salmonella enterica is a versatile live carrier for the presentation of recombinant vaccine antigens. fusion proteins of a type iii secretion system effector and heterologous vaccine antigens can be translocated by live attenuated salmonella strains and mediate protective immunity against infections. here we investigated the use expression cassettes for translocated fusion protein consisting of effector ssef and antigens of listeria monocytogenes after stable integration into the salmonella chro ... | 2009 | 19464562 |
| the single substitution i259t, conserved in the plasminogen activator pla of pandemic yersinia pestis branches, enhances fibrinolytic activity. | the outer membrane plasminogen activator pla of yersinia pestis is a central virulence factor in plague. the primary structure of the pla beta-barrel is conserved in y. pestis biovars antiqua, medievalis, and orientalis, which are associated with pandemics of plague. the pla molecule of the ancestral y. pestis lineages microtus and angola carries the single amino acid change t259i located in surface loop 5 of the beta-barrel. recombinant y. pestis kim d34 or escherichia coli xl1 expressing pla t ... | 2009 | 19465664 |
| relationships between multidrug-resistant salmonella enterica serovar schwarzengrund and both broiler chickens and retail chicken meats in japan. | we examined 29 isolates of salmonella enterica subspecies enterica serovar schwarzengrund from broiler chickens (n=19) and retail chicken meats (n=10) in japan for antimicrobial susceptibility and pulsed-field gel electrophoresis (pfge) profiling. all isolates exhibited resistance to both bicozamycin and sulfadimethoxine (minimum inhibitory concentration of both antimicrobial agents: >512 microg/ml). nalidixic acid resistance was found in only one broiler chicken isolate. pfge analysis showed th ... | 2009 | 19468180 |
| insights into the evolution of sialic acid catabolism among bacteria. | sialic acids comprise a family of nine-carbon amino sugars that are prevalent in mucus rich environments. sialic acids from the human host are used by a number of pathogens as an energy source. here we explore the evolution of the genes involved in the catabolism of sialic acid. | 2009 | 19470179 |
| outer membrane protein stm3031 (ail/ompx-like protein) plays a key role in the ceftriaxone resistance of salmonella enterica serovar typhimurium. | previously, the putative outer membrane protein stm3031 has been correlated with ceftriaxone resistance in salmonella enterica serovar typhimurium. in this study, this protein was almost undetectable in the ceftriaxone-susceptible strain 01-4, but its levels were increased in 01-4 isogenic strains for which mics were higher. the stm3031 gene deletion mutant, r200(deltastm3031), was generated and showed >64-fold lower ceftriaxone resistance than r200, supporting a key role for stm3031 in ceftriax ... | 2009 | 19470505 |
| extended-spectrum {beta}-lactamases and ampc {beta}-lactamases in ceftiofur-resistant salmonella enterica isolates from food and livestock obtained in germany during 2003-07. | detection and characterization of extended-spectrum beta-lactamases (esbls) and ampc-encoding genes was conducted in german salmonella isolated from different sources from 2003 to 2007. | 2009 | 19474065 |
| the effect of methylation on some biological parameters in salmonella enterica serovar typhimurium. | cell growth is tightly coupled to dna replication and its methylation [proc natl acad sci u s a 93 (1996) 12206-12211]. in a culture medium, growing of salmonella typhimurium (s. typhimurium) mutant cells (dam(-)) decreased (2.5 fold) relative to the wild type strain (dam(+)). in this study, we show that the reason for this growth deficiency is due to the dna methylation. the absence of a dam methyltransferase protein results in poor growth efficiency and disturbs the synchrony of replication in ... | 2009 | 19477083 |
| the base excision repair system of salmonella enterica serovar typhimurium counteracts dna damage by host nitric oxide. | intracellular pathogens must withstand nitric oxide (no.) generated by host phagocytes. salmonella enterica serovar typhimurium interferes with intracellular trafficking of inducible nitric oxide synthase (inos) and possesses multiple systems to detoxify no.. consequently, the level of no. stress encountered by s. typhimurium during infection in vivo has been unknown. the base excision repair (ber) system recognizes and repairs damaged dna bases including cytosine and guanine residues modified b ... | 2009 | 19478870 |
| gold screen-printed-based impedimetric immunobiosensors for direct and sensitive escherichia coli quantisation. | label-free electrochemical impedance immunosensors for the detection and quantification of escherichia coli (e. coli) using self-assembled monolayers (sams)-modified gold screen-printed electrodes (auspes) were developed. two different immunosensor configurations were tested and compared. in the first one, the immunosensing design was based on the covalent immobilization of anti-e. coli at auspes using the homobifunctional cross-linker 3,3'-dithiobis[sulfosuccinimidylpropionate] (dtssp). the oth ... | 2009 | 19481924 |
| [detection of bla ctx-m-14 and aac(3)-ii genes in salmonella enterica serotype kedougou in algeria]. | 2009 | 19482452 | |
| molecular characterization of the multidrug-resistant phage types salmonella enterica serovar typhimurium dt104, dt20a and dt120 strains in the slovakia. | an increase in the number of multidrug-resistant salmonella enterica serovar typhimurium strains (definitive phage type dt20a and dt120) as well as the occurrence of dt104 strains during 2003-2005 in slovakia was documented. based on the results of the molecular analysis we suggest that multidrug-resistant dt20a and dt120 phage types are more closely related to multidrug-resistant phage type, and that the occurrence is probably due to changes in the phage susceptibility of dt104. continued surve ... | 2009 | 17306968 |
| nitrous oxide emission and microbial community in the rhizosphere of nodulated soybeans during the late growth period. | we examined n(2)o emissions from the rhizosphere of field-grown soybeans during the late growth stage (99-117 days after sowing). marked emissions were detected from the nodulated root systems of field-grown soybeans, whereas a non-nodulating soybean mutant showed no emission. degraded nodules exclusively generated the n(2)o. a culture-independent analysis of microbial communities showed bradyrhizobium sp., acidvorax facilis, salmonella enterica, xanthomonas sp., enterobacter cloacae, pseudomona ... | 2009 | 21566356 |
| dry-resistance of salmonella enterica subsp. enterica serovar enteritidis is regulated by both sep22, a novel pathogenicity-related factor of salmonella, and nutrients. | environmental isolates of salmonella enterica serover enteritidis (s. enteritidis) clones were grown to the logarithmic phase, washed and re-suspended in saline or luria-bertani (lb) medium, and then 10-µl aliquots of the suspensions were dried overnight at room temperature. the dried bacteria were mixed with 1 ml of ice-cold pbs, suspended and examined for colony-forming activity. all of the pathogenic clones with high levels of sep22, identical to salmonella dps, maintained good viability if s ... | 2009 | 21566364 |
| evaluation of aroa deletion mutant of salmonella enterica subspecies enterica serovar abortusequi for its vaccine candidate potential. | the present study on a defined deletion aroa mutant (b-26) of salmonella enterica subspecies enterica serovar abortusequi (s. abortusequi) for residual virulence and safety in experimental model revealed that the virulence of the strain was at no difference in any of the cell assays (caprine alveolar macrophages, bovine alveolar macrophages, guinea pig blood mononuclear cells and horse blood mononuclear cells) than that of its parent virulence plasmid cured (s-787) and wild type (e-156) strains. ... | 2009 | 20099460 |
| cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in pseudomonas putida dot-t1e. | bacterial membranes constitute the first physical barrier against different environmental stresses. pseudomonas putida dot-t1e accumulates cyclopropane fatty acids (cfas) in the stationary phase of growth. in this strain the cfab gene encodes the main cyclopropane synthase responsible of the synthesis of cfas, and its expression is mediated by rna polymerase with sigma factor σ(38). we generated a cfab mutant of p. putida dot-t1e and studied its response to solvents, acid ph and other stress con ... | 2009 | 21261919 |
| baseline titer of widal in bijapur. | 2009 | 22010492 | |
| evanescent wave fiber optic biosensor for salmonella detection in food. | salmonella enterica is a major food-borne pathogen of world-wide concern. sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. since salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (trf) assay. anti-salmonella polyclonal antibody was immobilized onto the surface of ... | 2009 | 22346728 |
| validation of a predictive model describing growth of salmonella in enteral feeds. | the growth of salmonella enterica subs. enterica sorovar typhimurium at 25ºc was monitored in industrialized and hospital formulated enteral feeds and the results were used to validate the mathematical model of salmonella growth presented by the pathogen modeling program (pmp) 7.0 (usda-usa). the generation time of salmonella in enteral feeds ranged from 21 to 34.8 min and, the maximum growth rate (μmax) varied from 1.28 to 1.95 h(-1), resulting in a population increase from 5 to 6 log10 cycles ... | 2009 | 24031335 |
| mannanoligosaccharide agglutination by salmonella enterica strains isolated from carrier pigs. | type-1 fimbriae are associated with most salmonella enterica serovars and are an essential factor for host colonization. mannanoligosaccharides (mos), a prebiotic that is agglutinated by type-1 fimbriae, are proposed for the control of enterobacteria colonization and may be an alternative to salmonella control in pigs. the aim of this study was to evaluate the capability of porcine salmonella strains to adhere to mos in vitro. a total of 108 strains of salmonella sp. isolated from carrier pigs w ... | 2009 | 24031388 |
| salmonella enterica serotype dublin bacteraemia mimicking enteric fever. | salmonella enterica serotype dublin, a bovine adapted serotype, is the commonest cause of salmonellosis in cattle. salmonellosis in animals always presents a potential zoonotic threat. infected cattles serves as a source of infection to humans. we present here salmonella dublin bacteraemia in an elderly patient, with all the clinical details, due to the rarity of its occurrence. he was treated successfully with ciprofloxacin and his follow up period was uneventful. | 2009 | 19736411 |
| split marketing as a risk factor for salmonella enterica infection in swine. | on-farm reduction of salmonella carriage prevalence in pigs requires the identification of risk factors to direct interventions development. this study was designed to determine if split marketing of finishing pigs constitutes a risk factor for salmonella infections, by comparing salmonella prevalence in the first group of pigs selected for harvest ("first pull") versus the prevalence in the last group of pigs selected for harvest ("close out") from multiple commercial finishing lots. nine paire ... | 2009 | 19737062 |
| codependent and independent effects of nitric oxide-mediated suppression of phopq and salmonella pathogenicity island 2 on intracellular salmonella enterica serovar typhimurium survival. | here we show that the salmonella enterica serovar typhimurium phoq sensor kinase lessens the cytotoxicity of reactive nitrogen species (rns) generated by inducible nitric oxide synthase (inos) in the innate response of mononuclear phagocytic cells. this observation is consistent with the expression patterns of phop-activated genes during moderate nitrosative stress in the innate host response. in contrast, rns synthesized during high-no fluxes of gamma interferon (ifn-gamma)-activated macrophage ... | 2009 | 19737903 |
| comparison of the entericbio multiplex pcr system with routine culture for detection of bacterial enteric pathogens. | the entericbio system uses a multiplex pcr assay for the simultaneous detection of campylobacter spp., salmonella enterica, shigella spp., and escherichia coli o157 from feces. it combines overnight broth enrichment with pcr amplification and detection by hybridization. an evaluation of this system was conducted by comparing the results obtained with the system with those obtained by routine culture, supplemented with alternative pcr detection methods. in a study of 773 samples, routine culture ... | 2009 | 19726596 |
| use of toll-like receptor assays to detect and identify microbial contaminants in biological products. | toll-like receptor (tlr)-expressing cells, for the first time, detected and identified a microbial contaminant in a product made in escherichia coli using an old manufacturing process. it was suspected of having a microbial contaminant(s) because, although it tested negative by standard pyrogen assays, it was associated with adverse events in early clinical trials. the assay readout is the induction of nf-kappab and/or cytokines in response to tlr activation. four coded samples, labeled a to d, ... | 2009 | 19726599 |
| regulation of sulfur assimilation pathways in salmonella enterica serovar typhi upon up-shift high osmotic treatment: the role of uhpa revealed through transcriptome profiling. | salmonella species were the most deeply and extensively studied prokaryotes, which were used as useful prokaryotic models for the genetic analysis. salmonella enterica serovar typhi (s. typhi) is the etiological agent of typhoid fever, a major health problem in developing countries. uhpb/uhpa couple is a member of two-component regulatory system. it is considered that uhpb/uhpa controls the expression of the transport protein uhpt, which enables the cell to acquire phosphorylated sugars from its ... | 2009 | 19727945 |
| motility revertants of opggh mutants of salmonella enterica serovar typhimurium remain defective in mice virulence. | we recently demonstrated that osmoregulated periplasmic glucans (opgs) of salmonella enterica serovar typhimurium are required for optimal mouse virulence. however, lack of opgs also generated pleiotropic phenotypes such as reduced motility and slower growth rate under hypoosmotic growth conditions. whether the observed suboptimal virulence of opg mutants was due to reduced motility was investigated by isolating fully motile revertants of opggh mutants. motility revertants remained defective in ... | 2009 | 19727946 |
| effect of age on susceptibility to salmonella typhimurium infection in c57bl/6 mice. | ageing is associated with a decline in immune function, which predisposes the elderly to a higher incidence of infections. information on the mechanism of the age-related increase in susceptibility to salmonella enterica serovar typhimurium (s. typhimurium) is limited. in particular, little is known regarding the involvement of the immune response in this age-related change. we employed streptomycin (sm)-pretreated c57bl/6 mice to develop a mouse model that would demonstrate age-related differen ... | 2009 | 19729455 |
| o-antigen delays lipopolysaccharide recognition and impairs antibacterial host defense in murine intestinal epithelial cells. | although toll-like receptor (tlr) 4 signals from the cell surface of myeloid cells, it is restricted to an intracellular compartment and requires ligand internalization in intestinal epithelial cells (iecs). yet, the functional consequence of cell-type specific receptor localization and uptake-dependent lipopolysaccharide (lps) recognition is unknown. here, we demonstrate a strikingly delayed activation of iecs but not macrophages by wildtype salmonella enterica subsp. enterica sv. (s.) typhimur ... | 2009 | 19730692 |
| salmonella enterica serovar typhimurium exploits toll-like receptor signaling during the host-pathogen interaction. | salmonella survives and replicates in host cells by using a type iii secretion system to evade host immune defenses. the innate immune system plays an important role as a first line of defense against pathogens and is mediated in part by toll-like receptors (tlrs); however, the infection dynamics of salmonella enterica serovar typhimurium within macrophages stimulated with tlr ligands is poorly understood. we studied the infection dynamics of salmonella in murine macrophages previously exposed t ... | 2009 | 19720755 |
| perceptions, practices, and consequences associated with foodborne pathogens and the feeding of raw meat to dogs. | this study explored the impact of feeding raw meat to dogs on the fecal prevalence of several enteric bacterial zoonotic pathogens. campylobacter jejuni was isolated from 1/42 (2.6%) raw meat-fed dogs. salmonella enterica was isolated from 2/40 (5%) of the raw meat feeds, 6/42 (14%) raw meat-fed dog feces, none of the dogs that did not receive raw meat (p = 0.001), 4/38 (10.5%) of the vacuum cleaner waste samples from households where raw meat was fed, and 2/44 (4.5%) of vacuum cleaner waste sam ... | 2009 | 19721784 |
| validation of intervention strategies to control escherichia coli o157:h7 and salmonella typhimurium dt 104 in mechanically tenderized and brine-enhanced beef. | after three different outbreaks were linked to the consumption of nonintact meat products contaminated with escherichia coli o157:h7, the u.s. department of agriculture, food safety and inspection service published notice requiring establishments producing mechanically tenderized and moisture-enhanced beef products to reassess their respective hazard analysis and critical control point system, due to potential risk to the consumers. the purpose of this study was to determine the effectiveness of ... | 2009 | 19722392 |
| transepithelial activation of human leukocytes by probiotics and commensal bacteria: role of enterobacteriaceae-type endotoxin. | the goal of the current study was to clarify whether commercially available probiotics induce greater trans-epithelial activation of human leukocytes than do commensal, food-derived and pathogenic bacteria and to identify the compounds responsible for this activation. eleven different bacterial strains, and some of their pathogen-associated molecular patterns, were incubated apically on a confluent layer of intestinal epithelial cells (caco-2), which were basolaterally co-cultured with human mon ... | 2009 | 19714861 |
| real-time reverse-transcriptase polymerase chain reaction for the rapid detection of salmonella using inva primers. | recent outbreaks of salmonella linked to fresh produce emphasize the need for rapid detection methods to help control the spread of disease. reverse-transcriptase polymerase chain reaction (rt-pcr) can detect the presence of mrna (shorter half-life than dna) with greater potential for detecting viable pathogens. the chromosomally located inva gene required for host invasion by salmonella is widely used for detection of this pathogen by pcr. detection of salmonella was undertaken by real-time rt- ... | 2009 | 19715427 |
| acute acalculous cholecystitis complicating typhoid fever in an adult patient: a case report and review of the literature. | a case of typhoidal acalculous cholecystitis is described in a 31-year-old indian man, who was admitted with 4-day fever, abdominal pain, diarrhea and vomiting. on examination, he looked ill, but was conscious and febrile with icteric sclera. the right upper quadrant of the abdomen was tender. investigations showed high liver enzymes with high total bilirubin. abdominal ultrasound findings were consistent with the diagnosis of acalculous cholecystitis and salmonella enterica serovar typhi was is ... | 2009 | 19717100 |
| crystal structure of the salmonella enterica serovar typhimurium virulence factor srfj, a glycoside hydrolase family enzyme. | to cause infection, salmonella enterica serovar typhimurium uses type iii secretion systems, which are encoded on two salmonella pathogenicity islands, spi-1 and spi-2, the latter of which is thought to play a crucial role in bacterial proliferation in salmonella-containing vacuoles (scvs) after invading cells. s. typhimurium srfj, located outside spi-2, is also known to be involved in salmonella pathogenicity and has high amino acid sequence homology with human lysosomal glucosylceramidase (glc ... | 2009 | 19717598 |
| dam methylation controls o-antigen chain length in salmonella enterica serovar enteritidis by regulating the expression of wzz protein. | we reported previously that a salmonella enterica serovar enteritidis dam mutant expressing a truncated dam protein does not agglutinate in the presence of specific antibodies against o9 polysaccharide. here we investigate the participation of dam in lipopolysaccharide (lps) synthesis in salmonella. the lps o-antigen profiles of a dam null mutant (sedeltadam) and the salmonella serovar enteritidis parental strain were examined by using electrophoresis and silver staining. compared to the parenta ... | 2009 | 19717610 |
| inactivation of salmonella enterica serovar enteritidis on shell eggs by pulsed light technology. | this is a study on the efficacy of pulsed light (pl) technology for the inactivation of salmonella enterica serovar enteritidis on shell eggs. in preliminary studies on noble agar, a pl treatment of 0.7 j/cm(2) gave an inactivation of 6.7 log cfu/cm(2). photoreactivation of salmonella (0.5-0.7 log cfu/cm(2)) was observed. different results were obtained in eggs according to the state of the cuticle. when unwashed eggs were pulsed, 24 to 80% of the samples showed the maximum decontamination (3.6 ... | 2009 | 19720417 |
| emergence of fluoroquinolone-resistant strains of salmonella enterica in surabaya, indonesia. | typhoid fever remains a major health problem in developing countries. fluoroquinolones such as ciprofloxacin emerged as the 1st-choice treatment of enteric fever, including typhoid, in the 1990s. recently, salmonella typhi strains with resistance to ciprofloxacin have been increasingly reported in several countries, although the fluoroquinolone-resistant clinical strain has not been reported in indonesia. in the present study, we examined the drug susceptibility and the presence of gyra mutation ... | 2009 | 19631095 |
| rapid identification of salmonella enterica subsp. arizonae and s. enterica subsp. diarizonae by real-time polymerase chain reaction. | reptiles are popular as pets, leading to an increased risk of human infections due to uncommon salmonella strains including the arizona group (subspecies arizonae and diarizonae). we present a real-time arizona-specific polymerase chain reaction demonstrating 100% specificity and 99.6% sensitivity, offering savings in time and labor over traditional identification methods. | 2009 | 19631101 |
| innate immune gene expression differentiates the early avian intestinal response between salmonella and campylobacter. | salmonella enterica serovar typhimurium and campylobacter jejuni are major human pathogens, yet colonise chickens without causing pathology. the aim of this study was to compare intestinal innate immune responses to both bacterial species, in a 4-week-old broiler chicken model. challenged and control birds were sacrificed and tissue samples taken for histopathology and rna extraction. no significant clinical or pathological changes were observed in response to infection with either bacterial spe ... | 2009 | 19632728 |
| distribution of sulfonamide resistance genes in escherichia coli and salmonella isolates from swine and chickens at abattoirs in ontario and québec, canada. | sulfonamide-resistant escherichia coli and salmonella isolates from pigs and chickens in ontario and québec were screened for sul1, sul2, and sul3 by pcr. each sul gene was distributed differently across populations, with a significant difference between distribution in commensal e. coli and salmonella isolates and sul3 restricted mainly to porcine e. coli isolates. | 2009 | 19633109 |
| [a new method of designing genechip probe for frontier port detection of pathogenic microorganism]. | in the process of designing genechip probe for detecting pathogenic microorganism, the selection of single nucleotide polymorphism (snp) combination is of great importance. at present, there is no automatic design method. this work is hard and the result is not always well. a new approach for selecting snp combination is presented in this paper. genetic algorithm is used to search optimal solution on the basis of classification ability of snp combination, which is evaluated by the rough set theo ... | 2009 | 19634689 |
| interaction between host cells and septicemic salmonella enterica serovar typhimurium isolates from pigs. | salmonella enterica serovar typhimurium is an important pathogen in swine and is also a frequently reported zoonotic agent. the objective of this study was to characterize isolates of s. enterica serovar typhimurium associated with septicemia in swine and to compare them to isolates recovered from clinically healthy pigs. we were particularly interested in comparing the two groups of isolates for their ability to adhere to and invade host cells, to be phagocytized and survive in monocyte cells, ... | 2009 | 19710281 |
| kinetics of the natural, humoral immune response to salmonella enterica serovar typhi in kathmandu, nepal. | typhoid fever is a major public health problem in developing countries, conservatively estimated to occur in 17 million cases and be responsible for 200,000 deaths annually. we investigated the acquisition of natural immunity to salmonella enterica serovar typhi in a region where typhoid is endemic by testing sera from an age-stratified sample of 210 healthy participants in kathmandu, nepal, for bactericidal activity toward s. typhi and for anti-vi capsular polysaccharide antibodies. bactericida ... | 2009 | 19710294 |
| effect of mixed antimicrobial agents and flavors in active packaging films. | active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ... | 2009 | 19711918 |
| previously uncharacterized salmonella enterica genes required for swarming play a role in seedling colonization. | incidences of bacterial foodborne illness caused by ingestion of fresh produce are rising. instead of this being due to incidental contamination, the animal pathogen salmonella enterica utilizes specific molecular mechanisms to attach to and colonize plants. this work characterizes two s. enterica genes of unknown function: a putative periplasmic protein, stm0278, and a putative protein with a hydrolase in the c-terminus, stm0650. stm0278 and stm0650 are important for seedling colonization but a ... | 2009 | 19713240 |
| development and application of an electrochemical plate coupled with immunomagnetic beads (elime) array for salmonella enterica detection in meat samples. | salmonella is one of the main organisms causing outbreaks of foodborne illness, and meat is one of the major vehicles of salmonellosis throughout the world. a novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (elime array), is proposed for the detection of salmonella in meat samples. after an optimization study, using salmonella enterica serotype enteritidis as reference antigen, the ability of the method to interact with a large numb ... | 2009 | 19639993 |
| survival and growth of salmonella and vibrio in som-fak, a thai low-salt garlic containing fermented fish product. | fermentation of raw fish is a common process in asia for improvement of shelf life and safety, however, little is known about the survival of pathogenic bacteria in these products. raw fish may be contaminated with salmonella and vibrio species. the purpose of this study was to determine survival and potential growth of salmonella enterica serovar weltevreden, s. enterica serovar enteritidis, vibrio cholerae and v. parahaemolyticus as influenced by the preservation parameters (sodium chloride, g ... | 2009 | 19640599 |
| detection of enterobacter sakazakii and other pathogens associated with infant formula powder by use of a dna microarray. | pathogen detection is critical to the process of generating and testing powdered infant formula (pif). an obstacle associated with pif microbial surveillance is that most current procedures are time-consuming and labor-intensive. we have developed a rapid, dna microarray-based detection technique to identify 10 different pathogenic bacteria associated with pif contamination based on the 16s-23s rrna gene internal transcribed spacer (its) sequences and wzy (o antigen polymerase) gene. using this ... | 2009 | 19641057 |
| gamma radiation used as hygienization technique for foods does not induce viable but non-culturable state (vbnc) in salmonella enterica subsp. enterica serovar typhimurium. | gamma radiation has been widely used for hygienization of food products. whether gamma radiation stress induces vbnc state in salmonella is of great concern. therefore, the study was carried out to determine whether gamma radiation exposure induces vbnc state in salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium). the parameters tested were culturability on agar medium, transcriptional activity by rt-pcr, cytoplasmic membrane integrity, and direct viable count using live/dea ... | 2009 | 19641961 |
| salmonella enterica subclinical infection: bacteriological, serological, pulsed-field gel electrophoresis, and antimicrobial resistance profiles--longitudinal study in a three-site farrow-to-finish farm. | the aim of this surveillance was to study both salmonella spp. shedding patterns and the time course of serological response in farrow-to-finish reared pigs from a subclinically infected farm. antimicrobial resistance profile, molecular subtyping, and the relationship among the isolates were determined by pulsed-field gel electrophoresis (pfge). a farrow-to-finish farm of 6000 sows, with a history of salmonella typhimurium septicemia, was selected. a longitudinal bacteriological and serological ... | 2009 | 19642916 |
| transcriptional profiling avian beta-defensins in chicken oviduct epithelial cells before and after infection with salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis (se) colonizes the ovary and oviduct of chickens without causing overt clinical signs which can lead to se-contamination of the content and membrane of shell-eggs as well as hatchery eggs. the organism utilizes the salmonella pathogenicity island-2 encoded type iii secretion system (t3ss-2) to promote persistence in the oviduct of laying hens. in this study, reverse transcriptase-polymerase chain reaction (rt-pcr) was carried out to determine the expressio ... | 2009 | 19642979 |
| internalization of salmonella enterica in leaves is induced by light and involves chemotaxis and penetration through open stomata. | outbreaks of salmonellosis related to consumption of fresh produce have raised interest in salmonella-plant interactions leading to plant colonization. incubation of gfp-tagged salmonella enterica with iceberg lettuce leaves in the light resulted in aggregation of bacteria near open stomata and invasion into the inner leaf tissue. in contrast, incubation in the dark resulted in a scattered attachment pattern and very poor stomatal internalization. forcing stomatal opening in the dark by fusicocc ... | 2009 | 19648358 |
| antimicrobial resistance-conferring plasmids with similarity to virulence plasmids from avian pathogenic escherichia coli strains in salmonella enterica serovar kentucky isolates from poultry. | salmonella enterica, a leading cause of food-borne gastroenteritis worldwide, may be found in any raw food of animal, vegetable, or fruit origin. salmonella serovars differ in distribution, virulence, and host specificity. salmonella enterica serovar kentucky, though often found in the food supply, is less commonly isolated from ill humans. the multidrug-resistant isolate s. kentucky cvm29188, isolated from a chicken breast sample in 2003, contains three plasmids (146,811 bp, 101,461 bp, and 46, ... | 2009 | 19648374 |
| comprehensive identification of salmonella enterica serovar typhimurium genes required for infection of balb/c mice. | genes required for infection of mice by salmonella typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. inactivation of such genes produces attenuated s. typhimurium strains that have potential for use as live attenuated vaccines. a quantitative screen, transposon mediated differential hybridisation (tmdh), has been developed that identifies those members of a large library of transposon mutants that are attenuated. tmd ... | 2009 | 19649318 |
| purification, crystallization and preliminary x-ray analysis of flit, a bacterial flagellar substrate-specific export chaperone. | the assembly process of the bacterial flagellum is coupled to flagellar gene expression. flit acts not only as a flagellar type iii substrate-specific export chaperone for the filament-capping protein flid but also as a negative regulator that suppresses flagellar gene expression through its specific interaction with the master regulator flhd(4)c(2) complex. in this study, flit of salmonella enterica serovar typhimurium was expressed, purified and crystallized. crystals of semet flit were obtain ... | 2009 | 19652350 |
| selectively reduced intracellular proliferation of salmonella enterica serovar typhimurium within apcs limits antigen presentation and development of a rapid cd8 t cell response. | ag presentation to cd8(+) t cells commences immediately after infection, which facilitates their rapid expansion and control of pathogen. this paradigm is not followed during infection with virulent salmonella enterica serovar typhimurium (st), an intracellular bacterium that causes mortality in susceptible c57bl/6j mice within 7 days and a chronic infection in resistant mice (129 x 1svj). infection of mice with ova-expressing st results in the development of a cd8(+) t cell response that is det ... | 2009 | 19692639 |
| tumor invasion of salmonella enterica serovar typhimurium is accompanied by strong hemorrhage promoted by tnf-alpha. | several facultative anaerobic bacteria with potential therapeutic abilities are known to preferentially colonize solid tumors after systemic administration. how they efficiently find and invade the tumors is still unclear. however, this is an important issue to be clarified when bacteria should be tailored for application in cancer therapy. | 2009 | 19693266 |
| a comparative study of thermal and acid inactivation kinetics in fruit juices of salmonella enterica serovar typhimurium and salmonella enterica serovar senftenberg grown at acidic conditions. | acid and heat inactivation in orange and apple juices of salmonella enterica serovar typhimurium colección española de cultivos tipo (i.e., spanish type culture collection) 443 (cect 443) (salmonella typhimurium) and s. enterica serovar senftenberg cect 4384 (salmonella senftenberg) grown in buffered brain heart infusion (ph 7.0) and acidified brain heart infusion up to ph 4.5 with acetic, citric, lactic, and hydrochloric acids was evaluated. acid adaptation induced an adaptive response that inc ... | 2009 | 19694554 |