Publications

[synaptic interactions of individual motor neurons of the spinal cord of the carp].	the interaction between motoneurons was studied in the isolated spinal cord of the fish (cyprinus carpio) by the recording of elementary epsps evoked in a motoneuron by intracellular stimulation of adjacent motoneuron. these epsps are mediated mainly by electrical transmission as evidenced by their short or negligible latencies, stable amplitudes and bidirectional transmission between motoneurons. in some cells double-component elementary epsps were revealed which indicate dual (electro-chemical ...	1986	3012381
simple method for observing migrating leucocytes in the skin of mirror carp (cyprinus carpio l.).		1986	2953193
phospholipid dependency of carp brain and liver mitochondrial monoamine oxidase.	the effects of lipid-protein interactions on carp brain and liver mitochondrial mao with respect to substrate and inhibitor preference, thermostability and arrhenius parameters were studied and compared. treatment with phospholipase a2, c or d decreased mao activities towards 5-hydroxytryptamine (5-ht), beta-phenylethylamine and tyramine similarly, accompanied by great changes in their apparent affinities for mao, but not by changes in vmax values. minimum phospholipid binding to mitochondria mi ...	1986	2879700
clastogenic effects of five carcinogenic-mutagenic chemicals on the cells of the common carp, cyprinus carpio l.	the cytogenetic effects of various i.p. treatments with five carcinogenic-mutagenic chemicals and three doses for each (aflatoxin b1, aroclor 1254, benzidine, benzo[a]pyrene and 20-methylcholanthrene), were investigated in the cells of the common carp, cyprinus carpio. injection with distilled water and corn oil served as the two control groups. for detecting cytogenetic damage we used two test systems, chromosomal aberrations (ca) in kidney cells and micronucleated erythrocytes (m). at 48 hr af ...	1986	2877805
electrocardiograms recorded from the body surface of the carp, cyprinus carpio.	the electrocardiograms (ecgs) in five kinds of bipolar leads were recorded from the body surface of the carp under water. each component constituting the ecg wave could be detected by the present method. the potentials of the ecgs recorded by the present method were relatively small, the values being 2-64 microv. the electrical axis in most carp was directed toward the right lateral side.	1986	2876807
the in vitro metabolism of eulan wa new by liver homogenates from freshwater fish.	the rate of formation of pentachloro-2-aminodiphenylether (5-pad) from pentachloro-2-(chloromethylsulphonamido) diphenylether (6-pcsd) by goldfish liver homogenates was studied in a variety of buffers. the highest rate was observed when the homogenate was prepared and assayed in phosphate buffer containing dithiothreitol. the 5-pad forming activity of liver homogenate preparations from a variety of freshwater fish (goldfish, carp, pike, rainbow trout, perch and eel) showed contrasting species di ...	1986	2873942
presynaptic alpha-adrenoceptor mediated inhibition of the neurogenic cholinergic contraction in the isolated intestinal bulb of the carp (cyprinus carpio).	the effects of norepinephrine, epinephrine and clonidine on neurogenic cholinergic contraction were examined in the presence of a beta-adrenoceptor blocking agent, carteolol (5 x 10(-6) m), in the isolated intestinal bulb of the carp. norepinephrine, epinephrine (10(-9)-10(-6) m) and clonidine (10(-8)-10(-5) m) inhibited the contraction induced by low frequency (2 or 5 hz) transmural stimulation (tms) without inhibiting the contraction induced by acetylcholine (ach, 6 x 10(-8)-4 x 10(-7) m). met ...	1986	2871988
are beta-adrenergic receptors in ventricular muscles of carp heart (cyprinus carpio) mostly the beta-2 type?	the positive inotropic effect of isoproterenol was quantified in the presence of several beta-adrenergic blocking agents in ventricular strips of carp heart. isoproterenol had a concentration-dependent positive inotropic effect. the effect was markedly inhibited by propranolol and carteolol, but was extremely insensitive to atenolol. practolol totally failed to alter the effect. these results indicated that the positive inotropic effect of isoproterenol may be mediated by mostly beta-2 adrenergi ...	1986	2871986
sublethal effect of ammonia on certain biochemical and haematological indicators in common carp (cyprinus carpio l.).	carp (cyprinus carpio l.) fry were exposed for 3 weeks to a sublethal concentration of ammonia (0.102 +/- 0.059 mg nh3/dm3). ammonia induced considerable changes in the level of free amino acids and unfavourable changes in the blood, i.e. leucopaenia and eosinophy. the observed increase of the concentration of most amino acids in fish intoxicated with nh3 suggests that the process reflects detoxication of ammonia which takes place both in the brain and muscles.	1986	2869895
studies on immunological properties of enolase from carp muscles (cyprinus carpio) after chemical modification of some amino-acid residues.	after modification of histidine residues, enolase from carp muscles lost the ability to precipitate with antibodies against native enzyme. this ability remained after nitration of tyrosine residues, but the immunoelectrophoretic pattern--localisation and height of arcs--differed from that of native enolase. minor changes of this pattern were found in enolase with modified tryptophan residues.	1986	2430546
karyotypes of cyprinus carpio and leuciscus cephalus.	karyological investigations on the common carp (cyprinus carpio) and chub (leuciscus cephalus) are described. the diploid chromosome complement in the carp was found to be 98, with 50 meta- to submetacentric chromosomes, 48 subtelo- to telocentric chromosomes, and an arm number of 148. chromosomal studies on the chub revealed the diploid chromosome complement to be 50, with 34 meta- to submetacentric chromosomes, 16 subtelo- to telocentric chromosomes, and an arm number of 84.	1986	2428561
pituitary gland of the exotic carp, carassius carassius linn., with reference to the histocytology of the adenohypophysis in conjunction to male and female rhythms of reproduction.	the pituitaries of the exotic carp (carassius carassius) are studied at the light microscopic level, for the characterization of the adenohypophysial cell-types with particular emphasis to the gonadotropic potency of the pituitary in relation to the annual reproductive patterns. the gland in the fish is of the cranioleptobasic type. based on the classical staining methods and localization of the differential cell-types, the adenohypophysis of the fish can be subdivided into the rostral (rpd) and ...	1986	3803862
anaerobic wintering of crucian carp (carassius carassius l.)--ii. metabolic products.	in long-term experimental anoxia (up to 140 days) crucian carp excreted ethanol and acetic acid. the maximum concentration of ethanol found in fish blood was ca 0.1%. the excretion rate of ethanol at temperatures from -0.5 to +5 degrees c was ca 25 micrograms/g fish wet wt/hr, but increased rapidly with increasing temperature. at 2 and 5 degrees c the ethanol excretion rate was independent of fish size, but at 12 and 18 degrees c a higher rate was observed in smaller fish. the decrease of water ...	1986	2869864
telencephalic afferent nuclei in the carp diencephalon, with special reference to fiber connections of the nucleus preglomerulosus pars lateralis.	fiber connections of the nucleus preglomerulosus pars lateralis (pgl), which primarily provides afferent inputs to the telencephalon, were examined in carp by means of horseradish peroxidase tracing methods. the major afferent sources of pgl are the bilateral nucleus tuberis anterior and a few projections are found deriving from the ipsilateral nucleus ventromedialis thalami, nucleus centralis posterior, dorsal periventricular hypothalamus, and torus semicircularis. axons arising in the pgl can ...	1986	2429725
gap-junctional contacts of luminosity-type horizontal cells in the carp retina: a novel pathway of signal conduction from the cell body to the axon terminal.	l-type horizontal cells (271) of the carp retina were studied with the aid of intracellular injections of lucifer yellow and horseradish peroxidase (hrp). coupling among these cells was revealed by passage of the dye out of the injected cell. thick processes that crossed axons filled with lucifer yellow also contained the stain. these thick processes appear to be the axon terminals of other horizontal cells. serial reconstruction of an axon terminal by electron microscopy revealed two groups of ...	1986	3734163
the time-course of appearance and net accumulation of horseradish peroxidase (hrp) presented orally to juvenile carp cyprinus carpio (l.).	a sensitive enzyme-linked immunosorbent assay (elisa) technique was used in order to determine horseradish peroxidase (hrp) uptake from the carp gut. hrp was detected in blood plasma and various tissues within 15 min of oral intubation. the time-course of net accumulation (uptake-degradation) over a 2 hr period was recorded. the presence of hrp reached a maximum in the body tissues approximately 1 hr after intubation and on a microgram/g wet weight basis the order of accumulation within the tiss ...	1986	2875835
monooxygenase activity of fish liver in biomonitoring aquatic environment.	the hepatic biotransformation activity of four cultivated freshwater fish, amur carp (ctenopharyngodon idella), carp (cyprinus carpio), rainbow trout (salmo gairdneri) and tench (tinca tinca) were studied one day after catching the fish from ponds and a river in the south of gdr and in fish kept for two weeks in clean filtrated and deacidified water. aryl hydrocarbon hydroxylase, 7-ethoxycoumarin o-deethylase and 7-ethoxyresorufin o-deethylase activities fell to about 20% during the stay in clea ...	1986	3492989
purification of gonadotropic hormone from the pituitary of the african catfish, clarias gariepinus (burchell), and the development of a homologous radioimmunoassay.	glycoprotein gonadotropic hormone (gth) was purified from 2000 pituitaries of male and female african catfish, clarias gariepinus. the first step was chromatography on concanavalin a-sepharose followed by filtration on ultrogel aca 54, chromatography on deae-cellulose, and filtration on ultrogel aca 54, respectively. finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. the gonadotropic activity in the different fractions was characterized using two tests: the radi ...	1986	3536658
biology and histopathology of thelohanellus hovorkai achmerov, 1960 (myxosporea, myxozoa), a protozoan parasite of the common carp (cyprinus carpio).		1986	2953194
applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.).		1986	2953200
beta-2 adrenergic receptors are not only for circulating catecholamines in ventricular muscles of carp heart (cyprinus carpio).	whether beta-2 adrenergic receptors are only for the circulating catecholamines in isolated ventricular muscles of carp heart was studied. isoproterenol, epinephrine and norepinephrine had concentration dependent positive inotropic effects. the ed50 values for isoproterenol, epinephrine and norepinephrine were 6.23 +/- 1.9, 87.3 +/- 27.3 and 4500 +/- 580 nm, respectively. phentolamine did not alter the inotropic effects. tyramine increases the force of contraction and this action was completely ...	1986	2871987
hormones with adrenocorticotropic and opiate-like activities from the carp (cyprinus carpio) pituitary.	carp (cyprinus carpio) pituitary acetone powder was extracted with a mixture of water, hydrochloric acid and acetone. an acid acetone powder was formed by adding the pituitary extract into a large volume of chilled acetone and subsequently recovering the precipitate. the powder was subjected to ion exchange chromatography on cm cellulose. fractions adsorbed on the ion exchanger exhibited acth-like activity as evidenced in the ability to stimulate lipolysis in isolated hamster adipocytes and cort ...	1986	2879705
effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio.	the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ...	1986	2429432
preparation and some properties of carp heavy meromyosin.	trypsin fragments carp myosin heavy chain into components of lower molecular weight. these changes are concomitant with the loss of ca2+-atpase and a weakening in actin-myosin interaction. as heavy meromyosin (hmm) prepared from myofibrils is more homogeneous, the above changes are due to overfragmentation of heavy chain mass. amino-acid composition of carp and rabbit hmm's are similar, but differences exist in response to dtnb and incorporation.	1986	2434053
1h nmr spectroscopic studies of calcium-binding proteins. 2. histidine microenvironments in alpha- and beta-parvalbumins as determined by protonation and laser photochemically induced dynamic nuclear polarization effects.	the microenvironments of the histidines in three isoforms of ca(ii)-bound parvalbumin (carp, pi = 4.25; pike, pi = 5.00; rat, pi = 5.50) have been examined with 1h nmr techniques to probe their protonation characteristics and photochemically induced dynamic nuclear polarizability (photo-cidnp). the histidine at position 26 (or 25), present in all three of these proteins, shows absolutely no photo-cidnp enhancement of its c2h or c5h resonances. nor does this nonpolarizable histidine possess a nor ...	1986	3707913
resonance raman spectroscopic evidence that carp deoxyhemoglobin remains in a t-like quaternary structure at high ph: implications for cooperativity.	resonance raman spectroscopy shows the fe-proximal imidazole stretching band to shift from 215 to 219 cm-1 between human deoxyhemoglobin (deoxy-hb) and a hb sample which is 75% oxygenated, demonstrating that the t-r quaternary structure switch can be monitored by resonance raman spectroscopy in native hb at equilibrium. for deoxy-hb from carp, the band is at 215 cm-1 at ph 9 as well as ph 6, contrary to previous reports of an elevated frequency at high ph. the invariance of this frequency over a ...	1986	3707921
characterization of lens crystallins and their mrna from the carp lenses.	crystallins from carp eye lenses have been isolated and characterized by gel permeation chromatography, sds-gel electrophoresis, immunodiffusion and amino acid analysis. gamma-crystallin is the most abundant class of crystallins and constitutes over 55% of the total lens cytoplasmic proteins. it is immunologically distinct from the alpha- and beta-crystallins isolated from the same lens and its antiserum shows a very weak cross-reaction to total pig lens antigens. comparison of the amino acid co ...	1986	3707973
annual cyclical changes in the testicular activity of an indian freshwater major carp, labeo rohita (hamilton).	different male germ cells identified on the basis of histological and cytological characteristics in the testicular lobules of labeo rohita have been grouped into spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. the seasonal changes of the testis in l. rohita have been described according to its morphological peculiarities as well as to its variations in gonadal volumes, gsi values and frequency percentages of the different male germ cells occurring in t ...	1986	3710120
galactosyl-binding lectins from the tunicate didemnum candidum. purification and physicochemical characterization.	the plasma of the ascidian didemnum candidum possesses lectin activity directed toward galactosyl moieties. we report the purification by affinity chromatography, the physicochemical properties, amino acid composition, and partial n-terminal amino acid sequence of two galactosyl-binding lectins d. candidum lectins i and ii (dcl-i and dcl-ii) from the plasma of this protochordate species. both lectins were purified by affinity chromatography (on acid-treated sepharose 4b and asialofetuin conjugat ...	1986	3722194
radioimmunoassays for fish tail neuropeptides: i. development of assay and measurement of immunoreactive urotensin i in catostomus commersoni brain, pituitary, and plasma.	antisera were raised in rabbits using highly purified urotensin i (ui) or ui (4-41) conjugated with high-molecular-weight proteins. antiserum 2u4, at a final dilution of 1:300,000, gave 50% binding to iodinated ui (specific activity 40-120 microci/micrograms). carp (cyprinus carpio) and sucker (catostomus commersoni) ui peptides cross-reacted completely with the antiserum. results from cross-reactivity tests using various tryptic and staphylococcus aureus protease fragments of ui indicated that ...	1986	3724203
protein digestion and amino acid absorption along the intestine of the common carp (cyprinus carpio l.), a stomachless fish: an in vivo study.	protein hydrolysis to peptides and free amino acids and the apparent absorption of amino acids (aaaa) were evaluated in different segments of carp intestine. the aaaa analysed using cr2o3 as a marker indicated that 73.2% of the amino acids were absorbed in the first 20% of the intestinal tract and 5.3 and 21.5, respectively, in the following segments (20% of gut length). except for methionine and histidine, essential free amino acid concentration decreased significantly along the intestine. of t ...	1986	3749594
pronephric leucocytes of cyprinus carpio: isolation, separation and characterization.	since the teleost pronephros is an important source of diverse immunocytes, suspensions of pronephric cells from young adult carp have been characterized. in freshly prepared suspensions, adherent, spreading cells (macrophages?) constituted less than 3% of the total population. granulocytes and lymphocytes were co-dominant (less than 80%) leucocyte types. continuous percoll density gradient centrifugation yielded discrete subpopulations with these rho values and cytological characteristics: frac ...	1986	3765337
blood changes in carp (cyprinus carpio) induced by ulcerative aeromonas salmonicida infections.	only recently aeromonas salmonicida has been recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. our attempts to formulate a vaccine based on bacterial surface antigens were unsuccessful in conferring reliable protection against lethal challenge. this lead us to study pathological changes in the humoral defense system during ulcerative a. salmonicida infection in carp. high numbers of opportunist pathogens such as a. hydrophila and pseudomonas sp. were frequent ...	1986	3765352
does aeromonas salmonicida affect the immune system of carp, cyprinus carpio l.?	aeromonas salmonicida is a significant bacterial pathogen of cyprinid and salmonid fishes causing the systemic disease furunculosis. several observations led us to believe that a. salmonicida was able to evade or suppress the immune system of the fish: injection of whole bacteria or surface antigens was unsuccessful at protecting fish against lethal challenges; memory did not develop in survivors of sublethal infections; diseased fish often carried other opportunistic bacterial pathogens in addi ...	1986	3765353
the influence of environmental temperature and infection route on the immune response of carp (cyprinus carpio) to spring viremia of carp virus (svcv).	carp infected by water route and by injection of svcv developed neutralizing antibodies within 2-8 weeks at water temperatures between 10-20 degrees c. at 10 degrees c svcv persisted 12 weeks in the blood of carp although antibodies were present. immunized carp showed a solid protective immunity to svcv.	1986	3765360
analysis of zeros of binding polynomials for tetrameric hemoglobins.	a quantitative measure of the validity of the mwc description of cooperative binding equilibria has been obtained which uses only the adair constants. this is accomplished through simple relationships using the zeros of the adair binding polynomial and unique properties of the zeros of mwc polynomials as described in the accompanying paper (w.e. briggs, biophys. chem. 24 (1986) 311). the method is applied to oxygen binding to a large number of hemoglobins under a wide variety of conditions. in m ...	1986	3768473
immunization of carp (cyprinus carpio) with a vibrio anguillarum bacterin: indications for a common mucosal immune system.	uptake and transport of formalin-killed vibrio anguillarum bacteria were studied in the second gut segment of carp and the resulting reaction of the immune system was investigated. within a few hours after anal administration antigenic determinants of bacteria were present in intraepithelial macrophages of the second gut segment. in gut and skin mucus and bile, immunoglobulins (ig's) were detected, but the amount was much lower than found in serum. in mucus of the second gut segment, 4 times mor ...	1986	3770268
species variation of gonadotropic activity in an in vitro assay measuring androgen formation by carp (cyprinus carpio) testis with special reference to bioassay of piscine gonadotropins.	the objectives of this study were to establish a suitable and validated in vitro bioassay of piscine gonadotropins (gths) by using a carp testis androgen production system and to compare the androgenic responses in such an assay to gonadotropins from various vertebrate species. the testes from mature carp with gonadosomatic indices of 8-30% were used. androgen production was first compared with respect to methods for preparation of the carp testis (sliced, minced, homogenized, and collagenase-di ...	1986	3770434
[interaction of histone h1 molecules in a solution].	molecules of histones h1 isolated from the calf thymus, carp testicles and spermatozoa as well as trypsin-stable fragments of these proteins have been studied from the standpoint of their structure and interaction using methods of differential spectrophotometry, gel filtration and turbidimetry. the globular structure of histone h1 of the calf thymus is formed with an increase in the ionic strength of the medium and it is eluted as dimer with gel chromatography. with a considerable local increase ...	1986	3775878
development and validation of a salmon growth hormone radioimmunoassay.	a highly specific and sensitive radioimmunoassay (ria) for the measurement of plasma and pituitary growth hormone (gh) levels in salmonid fishes was developed using an anti-serum raised in rabbit against chum salmon (oncorhynchus keta) gh (sgh). pituitary extracts and plasma from chum, coho, masu, and amago salmon, and from rainbow trout and japanese charr, all exhibited displacement curves parallel to the sgh standard. samples from the eel, carp, goldfish, and tilapia, as well as plasma from hy ...	1986	3781223
morphology of physiologically identified bipolar cells in the retina of the tiger salamander, ambystoma tigrinum.	intracellular recordings of the light responses of bipolar cells were made in the isolated perfused retina of the larval tiger salamander by microelectrodes filled with a 1% solution of the enzyme horseradish peroxidase (hrp). two classes of bipolar cell were identified in terms of their responses to luminous spots and annuli centered upon their receptive fields: on-center cells, which depolarized in response to a centered spot and hyperpolarized in response to a concentric annulus, and off-cent ...	1986	3793974
the amino-terminal sequences of four major carp gamma-crystallin polypeptides and their homology with frog and calf gamma-crystallins.	four major gamma-crystallin subfractions have been isolated from the carp (cyprinus carpio) and their n-terminal sequences determined by edman protein sequencing. extensive homologies indicative of close relatedness in their primary structure were found in these four gamma-crystallin polypeptides. comparison of the carp n-terminal sequences with those of mammalian and amphibian gamma-crystallins also showed a high degree of homology present in their n-terminal segments despite the dissimilarity ...	1986	3803569
[clinical picture of partial monosomy of chromosome 11 q].	a ten months old male infant was found to have a de-novo-deletion of the long arm of chromosome 11 (q23----qter). with regard to previous reports the combination of the following signs seems to be pathognomonic for partial monosomy of the long arm of chromosome 11: trigonocephaly, abnormal palpebral fissure, epicanthus, broad and depressed nasal bridge, carp shaped mouth, low-set malformed ears and statomotoric retardation. cerebral atrophy, bns-seizures, hypsarrhythmia and persistent leukocytop ...	1986	3807920
mechanism of synthesis and localization of mitochondrial and cytosolic fumarases in rat liver.	fumarases in the mitochondrial and cytosolic fractions of rat liver were separately purified and crystallized. these two fumarases were not distinguishable in physicochemical, catalytic, or immunochemical properties. the sequences of seven amino acids in the c-terminal portions of the two fumarases were shown using carboxypeptidase p to be identical, i.e.-val-asp-glu-thr-ala-leu-lys-. the amino acid sequence of the n-terminal portion of the mitochondrial fumarase was determined by the edman meth ...	1986	3812085
cation binding to parvalbumin studied by 113cd and 23na nmr. peak assignment of rabbit (pi 5.5) parvalbumin.	cation binding to three apoparvalbumins was studied by means of 113cd nmr. the 3 parvalbumins that were investigated were carp pi 4.25, rabbit pi 5.5 and pike pi 5.0. the results showed that cd2+ ions bind to the ef and cd sites of carp apoparvalbumin pi 4.25 with about the same affinity. for rabbit (pi 5.5) apoparvalbumin, cd2+ binds preferentially to the ef site, while for pike (pi 5.0) apoparvalbumin, it was the cd site that exhibited somewhat higher affinity for cd2+. the effect of mn2+ on t ...	1986	3825402
internally transposed signal sequence of carp preproinsulin retains its functions with the signal recognition particle.	it is shown that the signal sequence of carp preproinsulin is functional with the dog pancreatic signal recognition particle (srp) both when present at its normal location at the amino-terminus of the protein or when engineered to an internal location. inhibition of translation by srp in the absence of microsomal membranes, reconstitution by srp of the translocation competence of high-salt inactivated microsomes and signal peptide cleavage all occur with the signal sequence being preceded by a h ...	1986	3000822
a signal sequence is required for the functions of the signal recognition particle.	it is shown that removal of the signal sequence of carp preproinsulin by gene technology abolishes the interaction between the polypeptide chain and the signal recognition particle with respect to both its functions: the inhibition of translation by the signal recognition particle in the absence of microsomal membranes, and the reconstitution of the protein translocation competence of high-salt inactivated microsomes. it is concluded that a signal sequence is absolutely required for the function ...	1986	3004463
complete nucleotide sequence of the matrix protein mrna of vesicular stomatitis virus (new jersey serotype).	the complete nucleotide sequence of the mrna of the matrix (m) protein of vesicular stomatitis virus [new jersey serotype, vsv(nj)] was derived from a cdna clone and mrna. the mrna is 758 nucleotides long (excluding polyadenylic acid) and encodes a protein of 229 amino acids. the predicted amino acid sequence was compared with that of the corresponding protein of indiana serotype [vsv(ind)] and a fish rhabdovirus, spring viremia of carp virus (svcv). an amino acid identity of 62% was found betwe ...	1986	3006343
[accumulation of cyclic adenosine monophosphate in the ovary of the eel (anguilla anguilla l.) in vitro under the effect of carp gonadotropin or ovine lutropin: kinetics and thermodependence].	cyclic amp (camp) in pieces of eel ovary was greatly increased in vitro by the gonadotropin (cgth) of carp, another teleost fish; after one hour at 20 degrees c, maximal stimulation = 31.7 and e.d. 50 = 0.08 micrograms/ml. ovine lutropin (olh) had less effect (maximal stimulation: 2.35; e.d. 50: 1.42 micrograms/ml); its action suggested that it involved a subfraction (olh/cgth rac) of the receptor-adenylate cyclase (rac) systems which mediate the action of cgth. another difference was the percen ...	1986	3012723
distribution of pancreatic elastase and metalloproteinase in vertebrates.	elastase-like enzymes were detected as zymogens in all of the pancreatic extracts from the gummy shark, bullhead shark, angel shark, smooth hammerhead, bestel, rainbow trout, carp, eel, japanese mackerel, yellowtail, sea bass, parrotfish, bullfrog, chicken, bluewhite dolphin, hog, rat, cat, and dog. the distribution of pancreatic elastase and metalloproteinase was examined on the basis of the effect of specific inhibitors on elastase like-activity in each extract. the results indicate that pancr ...	1986	3484693
phylogenetic studies on lymphokines. fish lymphocytes respond to human il-1 and epithelial cells produce an il-1 like factor.	the results obtained indicate, that catfish peripheral blood lymphocytes recognize and respond to human il-1. the second part of this report is dealing with a substance produced by carp epidermal cells with functional similarities to mammalian il-1.	1986	3490047
cadmium-binding protein (metallothionein) in carp.	when carp (cyprinus carpio) were exposed to 5 and 30 ppm cd in the water, the contents of cd-binding protein, which has low molecular weight, increased in the hepatopancreas, kidney, gills and gastrointestinal tract with the duration of exposure. this cd-binding protein was purified from hepatopancreas, kidney, gills, and spleen of carp administered 2 mg/kg cd (as cdcl2), intraperitoneally for 6 days. two cd-binding proteins were separated by deae-sephadex a-25 column chromatography. these prote ...	1986	3519201
immunohistochemical localization of urotensins i and ii in the caudal neurosecretory neurons of the carp cyprinus carpio and the sharks heterodontus japonicus and cephaloscyllium umbratile.	using antisera to urotensins i and ii (ui and uii), in the carp, cyprinus carpio, three types of caudal neurosecretory neurons were identified: those with both ui- and uii-immunoreactivities, those with only ui-immunoreactivity and those with only uii-immunoreactivity. the last type of neurons exceeded the other types in number, while neurons immunoreactive with both ui and uii antisera were relatively few. the axons of neurons of these three types terminated around the capillaries in the urophy ...	1986	3524503
neuropeptide y (npy) immunoreactive neurons in the retina of different species.	neurons displaying neuropeptide y (npy) immunoreactivity were found among amacrine cells in the retina of baboon, pig, cat, pigeon, chicken, frog, trout, carp and goldfish. the immunoreactive cell bodies were located in the middle and the innermost cell rows of the inner nuclear layer with processes forming one, two or three more or less well-defined sublayers in the inner plexiform layer. the location and the density of the sublayers varied with the species investigated. in the frog retina, bip ...	1986	3546220
[immunoenzyme (elisa) diagnosis of spring viremia in carp].	the enzymoimmunologic (elisa) method was used to diagnose spring viremia in carp. the specific antibodies to rhabdovirus carpio in the fish sera were detected by this method. reaction specificity was tested by the use of viral and control antigens, by examination of positive and negative sera and by serum-free control incubation. applying the elisa method, the antiviral antibodies can be detected after infection or vaccination: this method is therefore suitable for thorough studies and when choo ...	1986	3094221
immunological relationship among glycogen-protein complexes of different species.	glycogen-protein complexes from the muscles of celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. after immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. the immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes wi ...	1986	3109350
the thyroxine-stimulating activity is only present in the glycoprotein and not in the protein fraction of a carp hypophyseal homogenate.	the in vivo release of thyroxine (t4) in the carp (cyprinus carpio) and the in vitro release of t4 in the frog (rana ridibunda) from isolated thyroids were used as a bioassay to study the thyrotropic activity of carp hypophyses. the addition of up to 1 hypophyseal equivalent to the medium did not increase the in vitro release of t4 from isolated frog thyroids in november whereas 3 mu of bovine thyrotropin (b-tsh) was effective. however, when this experiment was repeated at the end of february, t ...	1986	3557057
thermodynamics of carp metmyoglobin unfolding.	the conformational free energy of carp lateral muscle metmyoglobin at 25 degrees c ph 8 is 9.0 +/- 0.5 kcal/mol as estimated from isothermal unfolding by both urea and guanidinium chloride. a novel procedure for the simultaneous analysis of acid and guanidinium chloride unfolding data is described. acid denaturation data suggest that binding of five protons to histidyl residues occurs on unfolding. correlation of sequences and conformational stabilities of several myoglobins according to the ter ...	1986	3956157
inhibitory effect of carp muscle trypsin inhibitor on mammalian pancreatic proteinases.	carp muscle trypsin inhibitor showed an inhibitory effect on bovine trypsin, bovine alpha-chymotrypsin and porcine elastase in a non-competitive, competitive and competitive manner, respectively. the inhibitor formed a stable complex with the above proteinases which was not dissociated in the presence of 2-mercaptoethanol and sds. the true target proteinase for carp muscle trypsin inhibitor, as yet unknown, seems to be an alpha-chymotrypsin- or elastase-like enzyme rather than trypsin, judging f ...	1986	3633776
in-vitro binding of gonadotrophin to fish ovary.	binding of piscine and mammalian gonadotrophin to plasma membranes from the ovaries of a fish, the murrel (channa punctatus), clearly suggests that the fish ovary possesses distinct and specific binding sites for both piscine and mammalian gonadotrophins. maximum specific binding of 125i-labelled human chorionic gonadotrophin (125i-hcg) and 125i-labelled silver carp gonadotrophin (125i-scg) was obtained at 30 degrees c and ph 7.5 during 2 h of incubation. in competitive binding studies, binding ...	1986	3643250
geminate recombination of co in rabbit, opossum, and adult hemoglobins.	the geminate recombination of co with hb following dissociation by a 10-ns laser pulse has been studied as a function of ph (9.2 and 7.0 without inositol hexaphosphate and 6.0 with inositol hexaphosphate) and temperature (5-35 degrees c). the hemoglobins studied included adult, rothschild, rabbit, opossum, and carp. despite significant differences in their structural and functional properties, the first four of these hemoglobins show similar trends in the yields, rates, and activation energies o ...	1985	3972802
[adaptibility of diplostomum cercariae in carp and the effect of a prior infestation].	experiments on the infection of carp fry with cercariae of diplostomum have shown that cercariae of diplostomum spathaceum can penetrate crystalline lens of larvae of these fishes in three days after their hatching and that the adaptability of cercariae of d. paracaudum to carp is much higher than that of cercariae of d. spathaceum. after the first infection with cercariae a relative postinfectious immunity against infection with cercariae of this or close species of this genus arises in carp fr ...	1985	3975067
non-specific cytotoxic activity of teleost leukocytes.	the existence of lymphoid cells with "natural" killer activity in mammals and birds has been known for some time. several previous reports have demonstrated similar activity in carp and catfish kidney leukocytes. however, the activity previously reported was directed towards established mammalian cell lines. in this report we confirm the existence of spontaneous killer activity in other species of teleosts, including salmonids. this spontaneous cytotoxic activity is directed towards several esta ...	1985	3996708
humoral response and memory formation in carp after injection of aeromonas hydrophila bacterin.	the humoral immune response of carp (cyprinus carpio) upon a bacterial fish pathogen aeromonas hydrophila was studied in relation to memory formation. after a single intramuscular (i.m.) injection of formalin killed a. hydrophila cells (f-ah), maximum serum ab (ab) titers were observed at day 20. distinct titers were still seen at day 360 in the groups injected with a medium or high antigen (ag) dose (10(7) respectively 10(9) f-ah). the effect of a second immunization with a high ag dose was stu ...	1985	3996709
pkx, the causative agent of proliferative kidney disease (pkd) in pacific salmonid fishes and its affinities with the myxozoa.	proliferative kidney disease (pkd), caused by an unclassified protozoan (pkx), is reported from pacific salmon, oncorhynchus tshawytscha (walbaum) and o. kisutch (walbaum), and steelhead trout, salmo gairdneri richardson, held at the mad river hatchery in california, usa. the cumulative mortality attributed to the disease was 95, 13, and 18% respectively. the mortalities were greatest at mean water temperatures of 12-14 degrees c during july 1983. the ultrastructure of the pkx organism and its a ...	1985	4009511
quaternary structure has little influence on spin states in mixed-spin human methemoglobins.	a key feature of the perutz stereochemical model for cooperativity in hemoglobin is a strong coupling between quaternary structure and the spin state of the heme iron [perutz, m. f. (1979) annu. rev. biochem. 48, 327-386]. while this coupling appears to be present for carp azide methemoglobin, it should also be present for all liganded forms of human methemoglobin that exhibit a thermal high-spin in equilibrium low-spin equilibrium. to test this hypothesis, we have measured the changes in spin e ...	1985	4016083
antibody diversity in fish. isoelectrofocalisation study of individually-purified specific antibodies in three teleost fish species: tench, carp and goldfish.	natural anti-dnp antibodies were isolated by affinity chromatography from individual sera of three cyprinid fish species (carp, goldfish and tench) and their electrofocusing (ief) spectra were analysed in reducing conditions. in addition, immune anti-penicillin and anti-bsa antibodies were isolated from individual and pooled tench sera, and studied by ief techniques on reduced samples. diversity rates appeared to be rather low in the three fish species, and striking similarities arose between in ...	1985	4018343
development and validation of a salmon prolactin radioimmunoassay.	a highly specific radioimmunoassay (ria) for the measurement of prolactin (prl) in the plasma and pituitary of salmonid fishes was developed using a rabbit antiserum to chinook salmon (oncorhynchus tschawytscha) prl. the prls purified from chinook salmon and chum salmon (o. keta) pituitaries showed exactly the same competitive inhibition curves in the ria, regardless of iodination of either hormone. the displacement curves for pituitary extracts and plasma from several salmonids, including chum, ...	1985	4018564
[metal concentrations in fishes of the rednitz river].	we investigated 39 fish species (eel, brown trout, chub, carp bream, roach, perch, pike etc.) from the river rednitz (north bavaria) for content of the metals mercury, lead, cadmium, arsenic, selenium and zinc. the residue tolerance level for mercury (1 mg/kg) and the standard value of the federal public health office for lead (0.5 mg/kg) and cadmium (0.05 mg/kg) have not been exceeded in any case. because a small selenium concentration gives protection against the toxicity of mercury, we also h ...	1985	4024745
a comparative study of tropomyosin from vertebrate ventricles.	a comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. the ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, but not among birds. the two components were concluded to be authentic tropomyosin and not artifacts since they showed lower electrophoretic mobilities in the presence of urea, and t ...	1985	4030730
[phylo- and ontogenic characteristics of lipid peroxidation in the retina of vertebrates].	phylo- and ontogenetic aspects of lipid peroxidation and antioxidative enzyme system in the retina of vertebrates were studied. it was established that both the intensity of lipid peroxidation and the activity of glutathione peroxidase in the retina of different vertebrate animals (carp, frog, tortoise, pigeon, rabbit) considerably diminished with evolution. the differences in the intensity of lipid peroxidation and the activity of glutathione peroxidase between dark- and light-adapted retinas a ...	1985	4052609
ca2+-specific fluorescence changes in n-dansylaminoethyl-labelled oncomodulin.	rat oncomodulin (isoelectric point (pi) = 3.9) and homologous carp parvalbumin (pi = 3.95) was labelled at cys-18 which is located in the ab loop, with the fluorescent reagent n-dansylaziridine. the fluorescence spectrum of the labelled parvalbumin was insensitive to both mg2+ and ca2+ binding. in contrast the dansyl fluorescence emission spectrum of labelled oncomodulin was quenched approximately 20% by ca2+ in the presence of physiological concentrations of mg2+. approximately 90% of the ca2+- ...	1985	4075234
antigen localization in the lymphoid organs of carp (cyprinus carpio).	a brief morphological description is given of the spleen, head kidney and trunk kidney, the three peripheral lymphoid organs of carp. an antigen-localization study was carried out using aeromonas hydrophila (a cellular bacterial antigen) and an indirect immunofluorescence test. examination of the lymphoid organs at various times after injection (up to 12 months) showed that antigen was at first present in splenic ellipsoids and in solitary phagocytic cells in the spleen, head and trunk kidney. l ...	1985	4075373
spectroscopic studies of protein-heme interactions accompanying the allosteric transition in methemoglobins.	resonance raman, optical absorption, and circular dichroism spectroscopic techniques have been used to examine the effect of the addition of inositol hexaphosphate (ihp) to a series of carp and human methemoglobin derivatives. markers of spin equilibrium in the high-frequency region (1450-1650 cm-1) of the resonance raman spectrum yield high/low-spin ratios consistent with direct magnetic susceptibility measurements. changes in the low-frequency region (100-600 cm-1) of the resonance raman spect ...	1985	4084499
purification and partial characterization of the glutathione s-transferases in carp liver, and their interaction with 2,4-dichlorophenoxyacetic acid and 1,4-benzoquinone.	the hepatic glutathione s-transferase (gst) activity in the cytosol of the freshwater fish carp (cyprinus carpio) was enriched by glutathione affinity chromatography. the anionic (gst a1-a3) and cationic (gst c1-c3) isoenzymes were then separated in two chromatofocusing steps. sds electrophoresis showed gst c1 to be a heterodimer with subunits of mr 25,000 and 28,000, and all other isoenzymes to be homodimers with subunits of mr 25,400. they were partially characterized by different biochemical ...	1985	4091850
electrophoretic and immunological study of myosin light chains from freshwater teleost fishes.	white muscle myosin light chains from nine freshwater teleosts show a qualitative and quantitative variability on page without phylogenetic correlation. they look different from their higher vertebrate counterparts mainly with regard to electric charge and relative amounts of alkali light chains corresponding to various contents of isoenzymic forms of white muscle myosin. antibodies against carp white myosin lc1 recognize almost entirely white muscle lc1 from the other fishes and to a lesser deg ...	1985	4092438
localization of immunoreactive synthetic atrial natriuretic factor (anf) in the heart of various animal species.	the localization of two synthetic fragments of the c-terminal portion of atrial natriuretic factor: arg 101-tyr 126 which displays full biological activity and leu 94-arg 109 which is completely devoid of biological activity, has been investigated by immunohisto- and immunocytochemical methods in the heart of mammals (rat, mouse, guinea pig, hamster, rabbit, cat, dog, man) and nonmammalian vertebrates toad (bufo marinus), frog (rana catesbeiana), fish (cyprinus carpio, puntius schwanenfeldi, cic ...	1985	3158698
quantitation and immunohistochemistry of catecholamines in the posterior segment of the eye.	dopamine, noradrenaline, and adrenaline were assayed with hplc in the light adapted retinae of carp, frog, chicken, pigeon, rat, guinea-pig, rabbit, cat, pig and cow. dopamine varied from 0.6 to 2.6 nmol/g wet weight and was not influenced by sympathectomy. the dopamine figures agree with previously published results. noradrenaline concentrations varied from not detectable to 0.06 nmol/g wet weight in different species. homolateral sympathectomy significantly decreased the noradrenaline figure i ...	1985	3875596
urotensin ii-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish.	antiserum generated against synthetic urotensin ii of the goby, gillichthys mirabilis, was used to localize urotensin ii in the caudal neurosecretory system in six species of freshwater teleosts: cyprinus carpio, carassius auratus, oreochromis mossambicus, oreochromis niloticus, salmo gairdneri and plecoglossus altivelis, and six species of seawater teleosts: acanthogobius flavimanus, pagrus major, parapristipoma trilineatum, trachurus japonicus, seriola dumerili and seriola quinqueradiata. in t ...	1985	3884162
immunohistochemical localization of chick retinal 24 kdalton protein (visinin) in various vertebrate retinae.	antiserum against a protein (24,000 daltons, visinin) of chick retina has been provided for immunohistochemical study on the localization of visinin in chick retinae during development, as well as in various vertebrate retinae. the photoreceptor cells were stained with anti-visinin serum from 7th day embryonic retinae and its intensity was gradually increased with embryonic age. in addition, visinin-like immunoreactivity was found in some kinds of amacrine and displaced amacrine cells from 11th- ...	1985	3886079
skeletal muscle actin as tumor marker in the diagnosis of rhabdomyosarcoma in childhood.	immunoaffinity-purified and tissue-specific antibodies against carp skeletal muscle actin have been assessed for their usefulness in diagnosing rhabdomyosarcoma. routinely processed formaldehyde-fixed tissue and the avidin--biotinyl--peroxidase complex technique were used. thirty-six tumors of patients varying in age from less than 1 year to 17 years were diagnosed as rhabdomyosarcoma on the basis of routine histological stains or electron microscopy, and on clinical grounds. among them were 20 ...	1985	3911779
microspectrophotometric and scanning microphotometric studies of carp (cyprinus carpio l.) erythrocytes.	carp (cyprinus carpio) hemoglobin readily autoxidizes in blood smears. quantification of soret-band absorbance in individual erythrocytes by means of scanning cytophotometry therefore requires more elaborate methods of preparation of blood samples. of the fixatives that have been tested, suspension of whole blood in isotonic salt solutions containing glutaraldehyde was most suitable. glutaraldehyde-fixed red blood cells are totally resistant to hemolysis. in the course of fixation, hemoglobin is ...	1985	3928536
opposite effects of ammonia and carbon dioxide on dye coupling between horizontal cells in the carp retina.	effects of ammonia (nh3) and carbon dioxide (co2) on the membrane potential of horizontal cells and on dye coupling between the cells in isolated retinas of the carp (cyprinus carpio) were investigated. ammonia (less than 300 ppm nh3 in air) initially depolarized and subsequently hyperpolarized, while co2 (10% in air) hyperpolarized the membrane potential of horizontal cells, accompanied by a diminution of both center and surround responses to spot and annular light stimuli. during the course of ...	1985	3929998
[reproduction of ichthyophthirius multifiliis (ciliata, ophryoglenidae)].	a case of reproduction of ichthyophthirius multifiliis in the superficial tissues of larvae and fry of amur wild carp is described. at a temperature of water from 28 to 29 degrees trophonts of ichthyophthirius encysted on fishes. inside cysts repeated cell division occurred but this process did not result in swarm spores formation. later on with the increase of temperature to 29.5--31.5 degrees cysts degenerated.	1985	3937118
reexamination of the evidence for paramagnetism in oxy- and carbonmonoxyhemoglobins.	experiments have been carried out in an attempt to confirm previous reports of paramagnetism in the oxy- and carbonmonoxy derivatives of human and carp hemoglobin. when care is taken to ensure complete saturation of the hemoglobins with ligand and the diamagnetic contributions of all of the buffers are carefully evaluated, these hemoglobin derivatives are found to have the same gram susceptibilities as exhibited by a set of metal free proteins, suggesting that they are fully diamagnetic.	1985	2982138
human chorionic gonadotrophin and immature fish ovary: characterization and mechanism of the in vitro stimulation of cyclic adenosine monophosphate accumulation.	human chorionic gonadotrophin (hcg) increased cyclic adenosine monophosphate (camp) in pieces of eel ovary in vitro. although the ed50 (about 0.1 micrograms/ml) was the same as for carp gonadotrophin (cgth), the maximal stimulation (close to 3 times basal level) was about 10 times lower than that for the fish hormone. the mechanism of this atypical action of hcg was studied using different approaches. human cg did not inhibit the action of cgth. desensitization experiments (cgth or hcg being inj ...	1985	2985465
purification and properties of carp (cyprinus carpio) muscle calpain ii (high-ca2+-requiring form of calpain).	calpain (ca2+-dependent cysteine proteinase) was purified to apparent homogeneity from carp muscle by the method of deae-cellulose, hydroxylapatite and ultrogel aca 34 column chromatographies. the purified enzyme is classified as calpain ii (high-ca2+-requiring form of calpain) from the effects of ca2+ concentration, ph and the antibiotics on the activity. carp muscle calpain ii was inhibited by rat liver calpastatin, the specific inhibitor for calpain. it is probable that the calpain-calpastati ...	1985	2992871
mutual inhibitory effect of calpastatins on calpains from carp muscle, carp erythrocytes and rat liver.	the inhibitory effect of calpastatin (specific inhibitor for calpain) on calpain (ca2+-dependent cysteine proteinase, ec 3.4.22.17) was examined using carp muscle, carp erythrocytes and rat liver preparations. a mutual inhibitory effect between calpains and calpastatins from different tissues and species was observed. the conservation of the inhibitory effect of calpastatin on calpain among vertebrates suggests that the calpain-calpastatin system may play a biologically fundamental and common ro ...	1985	2992872
viral diseases of fish: first report of carp pox in golden ide (leuciscus idus) in north america.	carp pox, a putative viral disease exotic to north america, occurred in golden ide 1 yr after the fish were imported into the united states from the federal republic of germany. the raised, white, plaque-like lesions, which occurred on about 5% of the fish, healed spontaneously and caused no mortality. electron micrographs showed herpesvirus-like particles associated with lesion specimens; however, no infectious viruses were detected in tests with seven warmwater fish cell lines.	1985	2993681
so-called interplexiform cells immunoreactive to tyrosine hydroxylase or somatostatin in rat retina.	the morphology of so-called interplexiform (ip) cells immunoreactive to tyrosine hydroxylase (th) or somatostatin (som) in the rat retina was described in comparison with those in the carp retina. in frozen cross-sections of the rat retina, many processes of th-like immunoreactive cells were found to extend toward the outer plexiform layer (opl), forming a thin layer of network fibers. a few of them further extended into the photoreceptor cell layer; such fibers were never found in the carp reti ...	1985	2864980
effects of acidic amino acid antagonists upon the spectral properties of carp horizontal cells: circuitry of the outer retina.	the acidic amino acid receptor antagonists, alpha-methylglutamate and alpha-aminoadipate, were applied to the carp retina to study their effects upon the spectral properties of horizontal cells and to elucidate the synaptic connections between horizontal cells and cones. application of these antagonists strongly hyperpolarized the l-type cone horizontal cells and reduced the responses of these horizontal cells to red light more than to blue light. application of co2+ ions to the retina, a proced ...	1985	2865339
histaminergic mechanisms in the vertebrate visual system.	this paper presents and summarizes our recent results on histamine (hi) in vertebrate visual system. the data include: 1. hi distribution, localization, synthesis, uptake and metabolism in retina, optic nerve, choroid and brain of carp, hen, rabbit, cow, 2. distribution of hi in human eyes, 3. 3h-mepyramine binding in the bovine retina, and hi dependent camp generating system in the rabbit eye, 4. effect of light stimulation on hi content in the rabbit retina, optic nerve and brain, 5. diurnal v ...	1985	2870485
[specificity of carp antihuman myeloma protein sera depending on the holding temperature].	while in carps kept in warm water (28 degrees c) and immunized with a human myeloma protein a challenge leads to an increase, the antibody titres in carps kept in cold water (14 degrees c) are changed only unsignificantly. antibody titres of carps immunized once respectively three times and kept in cold water are not markedly different. in testing the specificity of the antisera taken from each of the three groups no differences could be recognized. with carp anti-iggzei sera using the passive h ...	1985	2936221
production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain s-100 protein.	balb/c mice were immunized with s-100 protein, which was isolated from bovine brain. the first fusion resulted in the cloning of three stable hybridoma lines (s1-61-64, s1-61-65, s1-87-4) that produced monoclonal antibodies against s-100 protein. the hybridoma lines obtained from a second fusion (s2-20, s2-95) were not stable and antibody production ceased early during cloning. immunoblotting results showed that all antibodies reacted with antigenic determinants shared by both the alpha- and bet ...	1985	2578587
phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.	of eight monoclonal antibodies raised against human transferrin, one (h.tf-14) cross reacted with pig and rabbit transferrins and one (h.tf-1) showed cross-reactivity with horse and dog transferrins. while rabbit and pig transferrins exhibited the same patterns of binding to molt-3 cell receptors as human and horse transferrins, binding of mouse and dog transferrins was weaker and bovine and carp transferrins gave entirely negative results. the results of these competitive binding experiments we ...	1985	2578807
adenohypophysial cell types in an indian freshwater major carp, catla catla (hamilton).	the different adenocytes in an indian freshwater major carp, catla catla (hamilton) have been studied by employing various modern staining techniques. altogether, 8 different cell types have been reported in the adenohypophysis of the aforesaid fish. variations in the staining intensities of the cell types with the different techniques adopted have been detailed.	1985	2581848
a record of diplozoon nipponicum goto, 1891 found from cyprinus carpio nudus in korea.	diplozoon nipponicum, a monogenean trematode from cyprinus carpio was described according to the characteristic findings of x-form adult, metrical data, and presence of a pair of sticky glands. it is the first record of the presence of diplozoon nipponicum in korea.	1985	12888679
uptake and distribution of radioiodine, and the effect of ambient nitrate, in some fish species.	125i uptake by the thyroid was most pronounced in the smelt (osmerus eperlanus), perch (perca fluviatilis), rainbow trout (salmo gairdneri), roach (rutilus rutilus) and bleak (alburnus alburnus). unlike other tissues, only the muscle in the species studied no relative accumulation of 125i as compared to the ambient water. the crusian carp (cyprinus carassius) indicated the lowest levels of accumulation of 125i among the species tested. thyroid radioiodine concentration was independent of the len ...	1985	2858311
surface markers of male germ cells in early development of carp (cyprinus carpio l.) and the blood-testis barrier in fish. a study with monoclonal antibodies and horseradish peroxidase (hrp).		1985	4028174
peripheral and central distribution of major branches of the facial taste nerve in the carp.	the major pathways of the peripheral facial taste system in the carp, cyprinus carpio, are the maxillary (max), mandibular (mand), palatine (pal) and recurrent nerve rami. the peripheral distribution of the sensory fibers of these branches (b) was determined by means of electrophysiological techniques. max.b., mand.b. and pal.b., each of which arises from the gasserian-geniculate ganglionic complexes, were found to innervate respectively, the upper lip and the adjacent skin, the internal and ext ...	1985	3978434