Publications

presence of human papillomavirus type 16 related sequences in verrucous carcinoma of the larynx.	verrucous carcinoma of the larynx clinically resembles laryngeal papilloma in that both are wart-like masses on the vocal cords and may be characterized by multifocality and recurrence. human papillomavirus (hpv) infection is an etiological factor in laryngeal papilloma, and recent evidence implicates hpv in squamous neoplasias. to determine whether hpv is also associated with verrucous carcinoma of the larynx, we analyzed tissue specimens from six patients with verrucous carcinoma of the larynx ...	1986	3004723
human papillomavirus type 16 dna in genital tumours: a pathological and molecular analysis.	the presence of human papillomavirus type 16 (hpv16) dna in 34 genital tract tumours of italian female patients was investigated by southern blot hybridization in high stringency conditions. hpv16 dna was detected in 16 neoplasias, including cervical invasive and intraepithelial lesions as well as vulvar intraepithelial neoplasias and, to a lesser extent, vulvar invasive carcinomas. appropriate control tissues included in the study were negative. the data suggest that integration of viral dna ha ...	1986	3005483
cloning and characterization of the dna of a new human papillomavirus from a woman with dysplasia of the uterine cervix.	a previous analysis of 121 female genital tract lesions from the united states and south america had revealed that a large number contained dna sequences that were weakly homologous to a panel of human papillomavirus (hpv) probes. the dna sequences of one of these viruses have been molecularly cloned and shown to be a new type of hpv which is called hpv 31. among the cloned hpv genomes, hpv 31 is most closely related to hpv 16. although absent from all genital condylomas studied, hpv 31 was pres ...	1986	3005652
recombinant dna technology as it relates to human papillomavirus research.	recombinant dna technology has enabled investigators to understand papillomavirus biology. hpv dna has been found in a variety of benign and malignant tumors. once discovered, this hpv dna can be extracted, produced in large quantities, and submitted to nucleotide sequence analysis. ultimately, this research should reveal the mechanism(s) involved in the malignant transformation of hpv-induced papillomas. it may also help us produce a genetically engineered, safe vaccine against the hpv.	1986	2856310
in situ hybridization analysis of hpv 16 dna sequences in early cervical neoplasia.	the authors examined 18 cervical intraepithelial neoplasms (cin) for the presence of human papillomavirus (hpv) dna sequences by southern blot hybridization and dna-dna in situ hybridizations for hpv dna sequences and compared the epithelial distribution of hpv 16 dna sequences with hpv 6/11 sequences in selected condylomas. fifteen of the 18 cin lesions contained hpv 16 dna as determined by southern blot hybridization. with the use of biotinylated hpv 16 dna probes, 10 of the 18 were positive b ...	1986	2421580
association of human papillomavirus with squamous carcinoma of the uterine cervix.	the frequent association of human papillomavirus infection with preinvasive and invasive cancers of the cervix has led to speculation that the viruses may be involved in the neoplastic process. we have carried out some epidemiological studies of the prevalence of human papillomavirus infection in the female genital tract to evaluate more fully the oncogenic potential of these viruses in the cervix. a non-invasive method of detecting the virus has been developed for this purpose using dna hybridi ...	1986	2424677
epidermodysplasia verruciformis. skin carcinoma containing human papillomavirus type 5 dna sequences and primary hepatocellular carcinoma associated with chronic hepatitis b virus infection in a patient.	in a case of epidermodysplasia verruciformis with impaired cell-mediated immunity and multiple skin cancers human papillomavirus type 5 (hpv5) dna sequences were demonstrated in a cutaneous squamous cell carcinoma. hpv5 and hpv8 were detected in the benign disseminated skin lesions together with three newly characterized hpvs: hpv17, hpv19 and hpv24. a chronic infection with hepatitis b virus resulting in macronodular cirrhosis associated with a primary hepatocellular carcinoma was also acquired ...	1986	2426900
the l2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens.	the proteins encoded by the open reading frames of papillomavirus genomes and the minor polypeptides detected in purified virions are still poorly defined. we show here by its expression in escherichia coli that the open reading frame l2 of human papillomavirus type 1a codes for a minor structural protein of mr 76,000. antisera raised against a truncated l2-beta-galactosidase fusion protein in which the conserved n-terminal region of l2 is missing are type specific for human papillomavirus type ...	1986	2430112
quantitative assessment of langerhans' cells in human cervical intraepithelial neoplasia and wart virus infection.	langerhans' cell density was assessed quantitatively in cervical wart virus infection (cervical condyloma), cervical intraepithelial neoplasia, and koilocytic dysplasia with use of an antibody to s100 protein and an indirect immunoperoxidase technique. when compared with normal ectocervix, langerhans' cell density was significantly decreased in cervical wart virus infection and significantly increased in cervical intraepithelial neoplasia. in koilocytic dysplasia, intermediate langerhans' cell d ...	1986	3513580
detection of human papillomavirus types 6, 11, 16 and 18 in genital warts and cervical neoplasia.		1986	3034348
[infection of the oral mucosa with human papilloma virus (hpv)].		1986	3034514
langerhans' cells, papillomaviruses and oesophageal carcinoma. a hypothesis.	a hypothesis linking the concept of mucosal immune surveillance (langerhans' cells and intra-epithelial lymphocytes) with recent evidence of human papillomavirus (hpv) infection of the oesophagus in a sequence of events which leads to squamous dysplasia and invasive carcinoma is presented. it is believed that the essential pathway to squamous carcinoma involves an aberration of the langerhans' cell/lymphocyte network and its symbiotic relationship with squamous cells as a result of persistent hp ...	1986	3008355
the nucleotide sequence and genome organization of human papilloma virus type 11.	the complete nucleotide sequence of human papilloma virus type 11 (hpv11) dna (7931 bp) was determined. hpv11 dna which has been isolated from laryngeal papillomas and from genital warts (condylomata acuminata) shows a high degree of sequence homology to hpv6b (82%). the arrangement of open reading frames is very similar to hpv6b, the homology of the deduced amino acid sequences varies between 58 and 92%. characteristic features of the noncoding region between the l1 and e6 open reading frames i ...	1986	3008427
[human papillomaviruses in the epithelial cells of the cervix uteri: frequency of types 16 and 18. preliminary results of a clinical, cytologic and viral study].	between april 1 and june 30 1984, cervical scrapes were taken from 381 women attending the gynecology department of the anticancer center rené-huguenin. the scrapes were examined for the presence of human papillomavirus (hpv) dna, by a molecular hybridization method, at the pasteur institute. the four hpv types involved in genital pathology, hpv 6, hpv 11, hpv 16 and hpv 18, were studied. twenty four specimens (6.3%) were found positive: 19 for hpv 16, 3 for hpv 18, 2 for hpv 6 or hpv 11. result ...	1986	3009600
epidermodysplasia verruciformis-associated human papillomavirus 8: genomic sequence and comparative analysis.	human papillomavirus (hpv) 8 induces skin tumors which are at high risk for malignant conversion. the nucleotide sequence of hpv8 has been determined and compared to sequences of papillomaviruses with different oncogenic potential. the general organization of the hpv8 genome is similar to that of other types. highly conserved, genus-specific sequences were found in open reading frames (orfs) e1, e2, and l1. in orfs e6, e7, and l2, hpv8 is more distantly related, but it was possible to differenti ...	1986	3009874
coexistent chlamydial infections related to natural history of human papillomavirus lesions in uterine cervix.	to assess the role of chlamydia trachomatis in the development of cervical intraepithelial neoplasia (cin) and to evaluate possible synergism between chlamydiae and human papillomavirus (hpv) in this process, 418 women who had been prospectively followed up for cervical hpv infections at our clinic since 1981 were tested for chlamydiae. at each visit the patients were examined by colposcopy, and other investigations, such as papanicolaou (pap) smears, punch biopsies, urethral, and cervical swabs ...	1986	3021608
human papillomavirus types 16 and 18 sequences in early cervical neoplasia.	a total of 100 colposcopic biopsies from patients with abnormal papanicolau's tests were surveyed for the presence of human papillomavirus (hpv) types 16 and 18 sequences by spot-blot hybridization. hpv 16 and 18 dna sequences were detected in 58% of the biopsies. none of the cervical intraepithelial neoplasia grade i (cin i) contained hpv 16 while 50% of the cin iii lesions (carcinoma in situ, cis) contained hpv 16. hpv 18-related sequences were equally represented in cin i, ii, and iii. southe ...	1986	3022464
different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes.	transcription of human papillomavirus type 18 (hpv18) dna in the human cervical carcinoma cell lines hela, c4-1 and sw756 was studied by nucleotide sequence analysis of hpv18-positive cdna clones isolated from a hela, c4-1 and sw756 cdna library, respectively, and the cdna sequences were used to predict the potential encoded proteins. the cdna clones from all three cell lines were found to be derived from virus-cell fusion transcripts in which 3'-terminal host cell sequences (different for each ...	1986	3023067
genital tract papillomavirus type 6 in recurrent conjunctival papilloma.	an infant boy born of a mother who had condylomata (genital warts) during pregnancy and at delivery developed recurrent conjunctival papillomas and papillomas on the soft palate and the false vocal cords. a conjunctival lesion was first noticed by the mother when the infant was 4 months old and was excised and histologically diagnosed as a papilloma when he was 11 months old. the dna sequences of genital tract human papillomavirus type 6 (hpv-6) were identified in conjunctival papilloma tissue b ...	1986	3024608
bowenoid papulosis. a clinicopathologic study with ultrastructural observations.	one hundred eight patients were studied who had anogenital lesions showing microscopic features as seen in bowenoid papulosis (bp), a recently described condition occurring most commonly in young adults. patients typically show multiple papules, small nodules, or plaques that clinically mimic verrucae or nevocellular nevi. although the lesions show microscopic cytologic atypia, a distinction from bowen's disease, erythroplasia of queyrat, and other forms of carcinoma in situ can usually be made ...	1986	3002589
presence of human papillomavirus type-16 and type-18 dna sequences and their expression in cervical cancers and cell lines from japanese patients.	southern blot analyses of surgical specimens of cervical carcinoma from japanese patients showed that 3/9 samples contained human papillomavirus (hpv) type-16 dna sequences, and 2 contained hpv type-18 dna sequences. by northern blot analyses, rna transcripts of hpv dna sequences were demonstrated in some of the tissues containing hpv type-16 or hpv type-18 dna sequences. two cell lines established from cervical cancers of japanese patients also contained hpv type-18 genomes and these cell lines ...	1986	3007370
human papillomavirus and cervical neoplasia: epidemiological considerations.	recent clinical research using new diagnostic and laboratory techniques indicates that human papillomavirus (hpv) infection may play a key role in the development of cervical neoplasia. cervical hpv infection is found in 1-3% of routine papanicolaou smears, and preliminary data suggest that women with cervical hpv infection have about a tenfold greater risk of developing cervical intraepithelial neoplasia (cin) compared with those with no hpv infection. this paper discusses how epidemiological s ...	1986	3007381
bowenoid papulosis of the male and female genitalia: risk of cervical neoplasia.	sixteen patients with bowenoid papulosis (eleven male patients with bowenoid papulosis of the penis and five female patients with bowenoid papulosis of the vulva) were studied clinically, histologically, and virologically and followed up from 12 months to 5 years. in eleven of sixteen cases of bowenoid papulosis, molecular hybridization disclosed the presence of human papillomavirus type 16. in four cases we found new, not fully characterized human papillomavirus, and in two cases, we found both ...	1986	3007587
a comparison of the ages of patients with cervical smears showing human papilloma virus, dysplasia and carcinoma in situ changes.		1986	3010201
human papillomavirus infection of the cervix detected by cervicovaginal lavage and molecular hybridization: correlation with biopsy results and papanicolaou smear.	human papillomaviruses have previously been identified by molecular hybridization in the majority of dysplastic and cancerous lesions of the cervix. since human papillomavirus types 16 and 18 have been strongly associated with cervical cancer, the identification of patients infected with these specific human papillomavirus types may provide useful prognostic information. we have developed a painless, noninvasive cervicovaginal lavage technique to collect exfoliated cervicovaginal cells, which ca ...	1986	3010725
identification of the human papilloma virus-1a e4 gene products.	antibodies prepared against a human papilloma virus-1 (hpv-1) e4/beta-galactosidase fusion protein identified several polypeptides in hpv-1, but not hpv-2 or 4, induced papillomas. the major e4 protein, that represented up to 30% of total cellular protein, was a 16/17-k doublet which was purified by column chromatography and analysed for amino acid content. a peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. the unique sequence obtained, g ...	1986	3011404
peroxidase-antiperoxidase evaluation of human oral squamous cell papillomas.	in human beings, the viral origin of condylomatous lesions of the mouth and cervix, multiple papillomas, and verruca vulgaris of the larynx has been established. the viral origin of papillomatosis in rabbits and dogs has also been documented. the literature, however, shows no conclusive evidence of a viral origin of human oral squamous cell papillomas (scp). the purpose of this study was to use the peroxidase-antiperoxidase (pap) staining technique to determine whether human papillomavirus (hpv) ...	1986	3014421
natural killer (nk) cells with hnk-1 phenotype in the cervical biopsies of women followed-up for human papillomavirus (hpv) lesions.	local immunocompetent cell infiltrates in 263 cervical punch biopsies of women followed-up since 1981 (16 +/- 14 months, m +/- sd) for an established human papillomavirus (hpv) lesion with or without concomitant cervical intra-epithelial neoplasia (cin) were analysed for presence of human natural killer (nk) cells, defined by the monoclonal antibody hnk-1 (leu-7) using the avidin-biotin peroxidase complex (abc) technique. hnk-1+ cells remained at a constant low level (1.8 to 3.0% of the cells) i ...	1986	3014803
papillomavirus dna in warts of immunosuppressed renal allograft recipients.	renal allograft recipients were shown to have an increased incidence of warts and skin cancers. we examined 148 patients for evidence of wart virus infections and tested for papillomavirus types, which are known to be associated with human malignancies. of the 148, 36 (24.3%) patients were afflicted with warts at the end of our study period, in contrast to 5 of 148 (3.3%) before transplantation. dna from 16 different biopsies was extracted by phenol treatment for further virological studies. dna ...	1986	3015050
in vivo transformation of human skin with human papillomavirus type 11 from condylomata acuminata.	human papillomaviruses (hpvs) have been implicated in the development of a number of human malignancies, but direct tests of their involvement have not been possible. we describe a system in which human skin from various sites was infected with hpv type 11 (hpv-11) extracted from vulvar condylomata and was grafted beneath the renal capsule of athymic mice. most of the skin grafts so treated underwent morphological transformation, resulting in the development of condylomata identical to those whi ...	1986	3016303
detection of human papillomavirus type 16 dna and evidence for integration into the cell dna in cervical dysplasia.	the presence of human papillomavirus (hpv) type 16 dna in biopsies from precancerous lesions and from early lesions of human cervical cancer, and the integration of virus dna into host cell dna were analysed by dot blot and southern blot hybridizations. hpv 16 dna was detected in 23% of mild dysplasias, 32% of moderate dysplasias, 55% of severe dysplasias and 62% of carcinomas in situ by dot blot hybridization. digestion of the dna with restriction enzymes psti and bamhi followed by southern blo ...	1986	3018133
human papilloma virus and cervical cancer.		1986	3018462
human papillomavirus and cervical neoplasia.		1986	3018951
immunohistochemical and electron microscopic demonstration of human papillomavirus in dysplasia of the uterine cervix.	twenty-five cases of dysplasia of the uterine cervix were studied for the presence of human papillomavirus (hpv) by means of immunohistochemical and electron microscopic techniques. serial sections of the same histological specimen were examined in each case. hpv was detected in 14 cases by both immunohistochemistry and electron microscopy, while 10 cases were negative with both methods. in only one case, there was a discrepancy in the results derived from these two methods. it was concluded tha ...	1986	3018963
differential regulation of papilloma virus early gene expression in transformed fibroblasts and carcinoma cell lines.	treatment of bovine papilloma virus (bpv) 1-transformed mouse fibroblasts with cycloheximide led to a 10-fold increase in the amount of viral transcripts, after as little as 1 h of protein synthesis inhibition. northern blots revealed no qualitative changes in the rna pattern. nuclear run-on experiments showed about a 7-fold increase in specific transcriptional activity after cycloheximide treatment. the half-life of bpv1 mrna was twice as long as in untreated controls. these results indicate th ...	1986	3019673
atypical condylomas of the cervix uteri in singapore women: a histopathological and immunohistochemical study.	subclinical flat cervical warts are commonly recognized by means of colposcopy. these warty lesions frequently contain cellular atypia of a varying degree showing all the features of cervical intraepithelial neoplasia (cin) together with the features of wart virus infection e.g. koilocytosis. human papillomavirus (hpv) antigens and dna have been found in these lesions which can be called atypical condylomas. the presence of such markers in lesions regarded as having neoplastic potential implicat ...	1986	3021107
condylomas of the uterine cervix initially interpreted as squamous carcinoma: a report of four cases including a lesion resembling the buschke-loewenstein giant condyloma.	the recognition of human papilloma virus (hpv)-induced cervicovaginal condylomatous lesions is in a period of dynamic development. it is estimated that condylomatous involvement of the uterine cervix is epidemic and that is particularly true in the younger, more sexually active woman. while cervical condylomas are often associated with cervical dysplasia, carcinoma in situ, or even invasive carcinoma, they are sometimes misdiagnosed as these entities. four cases of cervical condylomas are presen ...	1986	3710268
the pathology of vulvar squamous cell carcinoma and verrucous carcinoma.	squamous cell carcinoma is far more common than verrucous carcinoma in the vulva. the clinical and morphologic distinctions between these neoplasms are important to understand because of their contrasting biologic behavior and treatment. both cancers present with symptoms of pruritus and a noticeable mass. on examination, both tumors commonly occur on the labia and are exophytic. if infection occurs in association with verrucous carcinoma, the resulting induration of the surrounding tissue as we ...	1986	3748617
cytologic detection of condylomas and cervical intraepithelial neoplasia of the uterine cervix with histologic correlation.	human papillomavirus (hpv) has been implicated as an important etiologic factor in cervical carcinoma. this study evaluates the efficacy of cytology as a screening tool in the detection of cervical lesions with koilocytotic features. cervical smears and biopsy specimens from 76 women seen between january 1983 and october 1985 were reviewed. the histologic categories consisted of koilocytotic lesions (flat condylomas) with minimal cellular atypia, cin i, ii, iii, with surface koilocytes showing c ...	1986	3756823
many oncogenes are transcribed in the d98ah2 derivative of the hela carcinoma cell line.	as a first step in an ongoing study on the correlation of oncogene expression and tumorigenicity in hybrids made by fusing d98ah2 (d98) carcinoma cells with normal diploid human fibroblasts, we determined which oncogenes are expressed in the d98 cells. northern blot determinations of the rna levels of 21 oncogenes revealed moderate to abundant transcripts of 13 oncogenes. the remaining eight oncogenes for which no rna was found normally show a very restricted pattern of expression among cell typ ...	1986	3780321
detection of human papillomavirus in epithelial cells of uterine cervix-frequency of types 16 and 18 hpv. preliminary results of a clinical cytological and virological study.		1986	3026741
human papillomavirus type 16 infection: a morphological spectrum with evidence for late gene expression.	human papillomavirus (hpv) type 16 is a unique strain found almost exclusively in squamous precancerous lesions and invasive carcinomas of the genital tract. a histological and immunohistochemical analysis of 34 cervical biopsies from which hpv 16 was isolated was performed to determine: the morphological spectrum of hpv 16 "infection" and if hpv late genes were expressed in lesions containing this virus. twenty-eight of thirty-four (82%) biopsy specimens contained areas fulfilling the histologi ...	1986	3026978
detection of human papillomavirus dna in oral mucosal lesions using in situ dna-hybridization applied on paraffin sections.	the in situ dna hybridization technique, carried out under stringent conditions, was used to detect human papillomavirus (hpv) dna of types 6, 11, and 16 in paraffin sections of 32 surgically treated oral mucosal lesions. expression of hpv structural proteins was analyzed by means of the immunoperoxidase (ip-pap) method. a total of 10 (31.3%) of the 32 lesions proved to express hpv antigens, which were found in 4 of 7 squamous cell papillomas, in 2 of 2 classic condylomas, in 2 of 10 papillary h ...	1986	3025793
treatment of recurrent squamous papillomata of the conjunctiva by carbon dioxide laser vaporization.	complete resection of recurrent conjunctival squamous papillomata frequently may be exceedingly difficult, since such lesions are typically friable and multilobulated. recurrent lesions often fill the fornicies and spill onto the lid margins. moreover, since the human papillomavirus (hpv) has been linked to some of these lesions, there remains the further possibility of spread with "cold knife" resection. we discuss the benefits of carbon dioxide laser vaporization therapy for recurrent squamous ...	1986	3093950
pathology: human papillomavirus infection and cervical neoplasia.		1986	18750081
human papillomavirus (hpv) type as an important determinant of the natural history of hpv infections in uterine cervix.	the present report summarizes our current observations on the natural history of cervical hpv (human papillomavirus) infections, based on data from 418 women prospectively followed-up in our clinic for a mean of 20 +/- 15 (m +/- sd) months. on each attendance at the clinic (at 6-month intervals), the patients are subjected to colposcopy accompanied by pap smears and/or punch biopsy, both being analysed for the cytopathic changes of hpv, and for concomitant cin (cervical intraepithelial neoplasia ...	1985	3021517
[cockayne syndrome--human papilloma virus and cervical cancer].		1985	3007848
sexual behaviour of women with human papillomavirus lesions of the uterine cervix.		1985	3002962
[recent advances in human papillomavirus research].		1985	3009929
diagnosis of cervical lesions from human papova virus.	the impact of human papillomavirus (hpv) infection in genital pathology has been clarified relatively recently and its relevance appears to be parallel to the liberalization of sexual customs. aside from some aspects of the infection grossly evident to the naked eye, an increasing number of sub-clinical lesions, that can be colposcopically identified as "flat condylomata" is commonly revealed by cytologic screenings. the authors report their experience in the diagnosis of this frequent pathology ...	1985	3832715
[epidemiologic factors in cervical intraepithelial neoplasia iii in our experience].	the study of 52 cases of cervical intraepithelial neoplasia seen from 1980 to 1984 shows that early sexual activity increases the relative risk 3.35 times if the intercourse takes place before 20 years of age, and 2.5 times if before 15 years. the use of iud increases the risk 2.77 times. parity, civil status and gynecologic infections do not increase the risk. cervical intraepithelial neoplasia is more frequently diagnosed in women with a higher socio-economic status, due to a better health car ...	1985	3880162
skin cancer in renal transplant recipients is associated with increased concentrations of 6-thioguanine nucleotide in red blood cells.	of 108 renal transplant recipients (53 men and 55 women) treated with azathioprine (0.8-2.9 mg/kg/day) and prednisolone (10 mg daily), 10 men had actinic keratoses, and five of these had squamous cell carcinoma, on light-exposed areas of skin. the time from transplantation to diagnosis of these skin lesions varied from 1.2 to 9.0 (mean 5.1) years. the concentration of the active azathioprine metabolite 6-thioguanine nucleotide was 120-425 (mean 276) pmol per 8 x 10(8) red blood cells in the tran ...	1985	3913458
progress toward the 1990 objectives for sexually transmitted diseases: good news and bad.	the problem of sexually transmitted diseases (stds) in the united states has been growing, in both scope and complexity, at an alarming rate. as evidence of the emergence of these diseases as a primary national concern, the surgeon general has designated them as 1 of 15 priority areas in which further actions are required to improve the health of the american people. the key targets for the 1990 objectives for the nation in the std area include reducing the incidence of gonorrhea; gonococcal pel ...	1985	3923531
improved laser techniques for the elimination of genital and extragenital warts.	two series of patients with genital warts were compared for effectiveness of treatment by means of the carbon dioxide laser. in the earlier series (1977 to 1981) laser vaporization was used to remove overt warts while surrounding normal skin was spared. examination of anal, urethral, and other extragenital sites for possible involvement was done only when warts persisted or recurred. in the later series (1983 to 1985), patients were similarly treated by carbon dioxide laser techniques; however, ...	1985	4061516
screening for wart virus infection in normal and abnormal cervices by dna hybridisation of cervical scrapes.	cervical scrapings from 78 women attending a sexually transmitted diseases (std) clinic, family planning clinic, and colposcopy clinic were assayed for human papillomavirus type 6 (hpv-6) by dna hybridisation. results of hybridisation were compared with clinical, colposcopic, and cytological findings. scrapings from 2 of the 4 women with genital warts gave positive results with the hpv probe. no hpv-6 dna was detected in scrapings from 18 women attending an fpc whereas scrapings from 2 of 19 std ...	1985	2857023
increased risk of cervical neoplasia in consorts of men with penile condylomata acuminata.	19 (76%) of 25 women who had been the sole sexual consorts, for at least 1 year, of men with pre-existing penile condylomata acuminata had similar lesions of the lower genital tract. 9 (36%) of the women had an abnormal cervix as judged by cytology, colposcopy, and histology, and 7 (77%) of these 9 had associated human papillomavirus (hpv) infection detected by dna/dna hybridisation. none of 20 women in an age-matched control group had cytological or colposcopic evidence of cervical epithelial a ...	1985	2859410
[immunohistochemical study of condyloma and and carcinoembryonic antigens in the uterine cervix in human papilloma virus infection and neoplasia].		1985	3006200
colposcopy in women with papillomavirus lesions of the uterine cervix.	colposcopic examinations for human papillomavirus lesions were performed in 271 women, some of whom had developed concomitant cervical intraepithelial neoplasia since 1981. the colposcopic appearance was classified into one of the following categories: normal, punctate, mosaic, warty, leukoplakial, or combination, and was related to findings in papanicolaou smears and punch biopsy specimens. there was a good correlation between the colposcopic appearance and the findings in the papanicolaou smea ...	1985	2983274
human papillomavirus type 16 related dna in an anaplastic carcinoma of the lung.	twenty-four biopsy specimens from various histologic types of human carcinomas in the lung were analyzed for the presence of human papillomavirus (hpv) dna. dna from the individual specimens was tested for the presence of homologous sequences to hpv genotypes 1, 2, 4, 8, 9, 10, 11, 13, 16 and 18. one anaplastic carcinoma in the lung contained multiple copies of dna hybridizing under stringent conditions to hpv 16 dna. the latter dna has been found to be frequently associated with human genital c ...	1985	2983861
[immunoperoxidase demonstration of papillomavirus antigen in dysplasia of the uterine cervix].	recently, it has been widely recognized that human papillomavirus (hpv) infection plays an important role in the etiology of carcinoma of the cervix. paraffin sections of cervical malignancies and premalignancies were screened for the presence of papillomavirus antigen by means of peroxidase-antiperoxidase (pap) method. no case of invasive carcinoma, carcinoma in situ or normal cervix revealed positive hpv staining. in 81 cases (18%) of 461 cervical dysplasias, positive reaction for hpv was dete ...	1985	2984298
[electron microscopic detection of viruses in cervix papilloma].	the authors describe the electron microscope findings in a case of papilloma of the uterine cervix. in the nuclei of single koilocytes spherical inclusions measuring 47-56 nm were found. the ultrastructural pattern of the particles is entirely identical with one of the human papilloma virus particles.	1985	2984862
papillomavirus infection of the lower genital tract: detection of viral dna in gynecological swabs.	a total of 311 smears from the lower genital tract were examined by the filter in situ hybridization method to identify human papillomavirus (hpv) dna. of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. in this group hpv-dna was detected in 5 cases (2.2%). of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained hpv-dna. a high prevalence of hpv 6/11 and absence of hpv 16/18 was found in cases with cytological ...	1985	2985509
analysis of oral papillomas, leukoplakias, and invasive carcinomas for human papillomavirus type related dna.	five papillomas, five leukoplakias, and six carcinomas were investigated for the presence of papillomavirus group-specific antigens and viral dna. viral proteins were identified with genus-specific papillomavirus antibodies. cloned human papillomavirus (hpv) 11 and 16 dna were used as probes in southern blot hybridization at conditions of different stringency in order to determine viral dna. four of five papillomas, four of five leukoplakias, and three of six carcinomas reacted with hpv dna prob ...	1985	2987361
introduction of cloned human papillomavirus 1a dna into rat fibroblasts: integration, de novo methylation and absence of cellular morphological transformation.	the morphologically normal rat fibroblast cell line rat-2 was used as a target cell type to test the transforming ability of a human papillomavirus (hpv-1a). to this end, molecularly cloned hpv-1a genomes were introduced into cultured rat-2 cells in cotransfection experiments using a cloned herpes simplex virus thymidine kinase gene as a selectable phenotypic maker. in each of 13 hpv-1a-positive cell clones examined the papillomavirus dna sequences were associated with the high molecular weight ...	1985	2987396
[detection of various papilloma virus types in cytologic smears of precancerous conditions and cancers of the uterine cervix].	dna-in situ hybridisation on epithelial cells taken from cervical swabs of 101 different patients cytologically diagnosed as iii d to v (munich classification 1975) were performed using 32p-labelled dna of the human papilloma virus (hpv) types 6, 11, 16 and 18 as smears. the following correlations between the cytological classification and dna hybridisation were obtained: 55 out of 58 women (94.8%) having a normal pap smear (pap ii) were negative by hybridisation as well, three cases contained h ...	1985	2989076
bowenoid papulosis. presence of human papillomavirus (hpv) structural antigens and of hpv 16-related dna sequences.	this study reviews 39 cases of anogenital bowenoid papulosis lesions in 22 individuals of both sexes that were analyzed clinically, histologically, immunocytochemically, and virologically. macroscopically, three different types of lesions were demonstrated: erythematous macules; papules (lichenoid and/or pigmented papules); and leukoplakialike lesions. microscopically, bowenoid papulosis fulfills the criteria of a squamous cell carcinoma in situ. much like oral precancers, three distinct growth ...	1985	2990353
papillomavirus genomes in human cervical tumors: analysis of their transcriptional activity.	four of six human cervical carcinoma biopsies were shown to contain the dna of the human papillomavirus type 16 (hpv-16) covalently linked with the tumor cell dna. hpv-16-specific mrna species were observed in only one of the four tumors. no such sequences were found in the three other specimens in conditions that permitted the detection of less than one mrna molecule per cell. it is concluded that maintenance of the malignant nature of these cervical tumors does not depend on the continuous tra ...	1985	2991933
comparison of prevalence of human papillomavirus antigen in biopsies from women with cervical intraepithelial neoplasia.	human papillomavirus antigen was found in 39 (16%) of 253 colposcopic biopsies from a group of women at high risk for cervical cancer who had been examined in the early 1970s. immunohistochemical evidence of papillomavirus infection was found in 20 (30%) of 67 cervical intraepithelial neoplasia lesions infected with wart virus from these patients. when these results were compared with results of a similar study carried out in the early 1980s there was no significant difference in the prevalence ...	1985	2993370
human papillomavirus dna in female condylomata.	we have studied the presence and state of human papillomavirus (hpv) genomes in condylomata acuminata of the vulva, vagina, and cervix and in flat condylomas of the cervix from japanese females by dna hybridization with hpv 6 and 11 dnas. unintegrated hpv 6-related dna (hpv 6 or 11 dna) was detected in 55 out of 60 condylomata acuminata and in 1 out of 4 flat condylomas under stringent hybridization conditions. the hpv dnas were identified as hpv 6 dna in 29 condylomata acuminata (20 vulvar, 3 v ...	1985	2993453
detection and localization of human papillomavirus dna in human genital condylomas by in situ hybridization with biotinylated probes.	we have examined the distribution of human papillomavirus (hpv) dna in paraffin sections of humans warts by in situ hybridization with biotin-labeled dna probes. recombinant plasmid dnas (hpv-1, -6, -11, -16) were labeled by nick translation with biotinylated deoxyuridine triphosphate. paraffin sections were hybridized with the probes for 18 h in stringent or non-stringent conditions, and dna-dna hybrids were detected by immunocytochemistry. paraffin sections of warts were also examined for the ...	1985	2993496
occurrence of human papillomavirus types 16 and 18 dna in cervical carcinomas from japan: age of patients and histological type of carcinomas.	we have detected by dna hybridization human papillomavirus (hpv) types 16 and 18 dna sequences in 19 and 3, respectively, out of 56 cervical carcinomas from japan. eighteen out of 19 hpv 16-positive specimens were from squamous cell carcinomas, whereas the three hpv 18-positive specimens were from a squamous cell carcinoma (1/50), an adenosquamous carcinoma (1/3), and an adenocarcinoma (1/3). the occurrence of hpv 16 dna decreased in patients over 60 years old (less than or equal to 60 years, 15 ...	1985	2995290
reproducibility of the cytologic diagnosis of human papillomavirus infection.	as part of a larger epidemiologic investigation of the association between human papillomavirus (hpv) infection and cervical intraepithelial neoplasia, the reliability of the cytologic diagnosis of hpv infection was examined. a random sample of cervicovaginal specimens with cytologic changes characteristic of hpv infection were matched with a second set of slides, with regard to the date and severity of the smear and the age of the woman from whom the smear was obtained. the kappa statistic for ...	1985	2996269
verrucous carcinoma of the larynx. possible human papillomavirus etiology.	verrucous carcinoma of the larynx is a distinct and uncommon variant of well-differentiated squamous cell carcinoma. by dna hybridization techniques, we clearly demonstrated human papillomavirus (hpv-16-related) sequences in five patients with this neoplasm. in addition, hpv-16-related sequences were found in adjacent normal tissues. the dnas from squamous cell carcinomas of the larynx were negative when hybridized to hpv-6, -11, or -16. postirradiation anaplastic transformation of verrucous car ...	1985	2996475
specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: a preliminary communication.	cervical papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral dna sequences by in situ hybridization technique using 35s-labeled cloned recombinant dna probes of human papillomavirus (hpv) types 6, 11, and 16. these and one unrelated dna probe complementary to measles virus rna were labeled by nick translation using either one or two 35s-labeled nucleotides. the radiolabeled probes were reduced in size with dnase to ...	1985	2997054
[human papilloma virus. relation to cervical neoplasia].		1985	3001991
[heck's disease].	the clinical, microbiological, histological and electron microscopical findings in cases of oral papillomatosis (heck's disease, focal epithelial hyperplasia), as well as differential diagnosis and therapy are presented. electron microscopic investigations confirmed the presence of human papilloma virus (hpv) in the lesions of heck's disease.	1985	3002054
human papillomavirus dna in genital tumours.	human papillomaviruses (hpv) types 16 and 18 have been identified in two different human cervical carcinomas. the viral dnas were molecularly cloned and used as probes to screen a large number of genital tumours by southern blot analysis. hpv 16 or hpv 18 sequences were found in a high percentage of cervical carcinomas but only in a small number of condylomata acuminata or flat condylomas. the majority of the latter lesions, however, contained hpv 6 or hpv 11 sequences, respectively, which, in c ...	1984	6100278
monoclonal antibodies to the major capsid protein of human papillomavirus type 1.	two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (hpv-1) were isolated. the monoclonal antibodies detected hpv-1 antigens in frozen sections of hpv-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with hpv-1 particles in an immunodiffusion test. the two monoclonal antibodies recognized the major structural viral polypeptide, with a molecular weight of 54 000, and a minor polypeptide, wi ...	1984	6086818
the koilocyte and cervical intraepithelial neoplasia: time-trend analysis of a recent decade.	to determine whether the role of the human papillomavirus in the development of cervical intraepithelial neoplasia has changed recently, cervical biopsy specimens from the years 1972 and 1982 were reviewed to compare the frequency of diagnosis of human papillomavirus-associated koilocytosis in patients with suspected cervical intraepithelial neoplasia. no significant difference was found in the frequency of koilocytosis in biopsy specimens with cervical intraepithelial neoplasia between the year ...	1984	6091461
[cytologic diagnosis of cervico-vaginal infections with herpesvirus hominis type ii and human papilloma virus].		1984	6091487
molecular cloning and characterization of the genomes of nine newly recognized human papillomavirus types associated with epidermodysplasia verruciformis.	the genomes of 11 human papillomaviruses (hpvs) found in benign lesions of eight patients suffering from epidermodysplasia verruciformis were cloned in escherichia coli after insertion into plasmid pbr322. the study of the sensitivity of the cloned hpv dnas to 14 restriction endonucleases permitted the construction of physical maps. dna-dna hybridization experiments, performed under stringent conditions, showed that these viruses represent nine new types, hpvs 14 (with subtypes a and b), 15, 17 ...	1984	6092701
[focal epithelial hyperplasia (heck disease). contribution on its viral origin].	demonstration of human papilloma virus dna (hpv-dna) in the lesions of focal epithelial hyperplasia in an 11-year-old turkish girl provides further proof of its viral cause. in this case characterization of hpv-dna showed a reaction with that of type 13 and 18. this previously undescribed variant is probably the expression of a new hpv type.	1984	6094135
cloning and characterization of papillomavirus type 2c dna.	human papillomavirus (hpv) dna was isolated from a clinically diagnosed flat wart and proved to be related to hpv2. the isolate showed 55% cross-hybridization with hpv2a. a physical map of restriction enzyme cleavage sites differed completely from those of hpv2a and hpv2b. the new hpv2 subtype, which will be classified as hpv2c, was found to be very prevalent in common warts.	1984	6094390
langerhans cells in human papillomavirus (hpv) lesions of the uterine cervix identified by the monoclonal antibody okt-6.	the local inflammatory cell infiltrates in 263 cervical punch biopsies of the women followed-up since 1981 (16 +/- 14 months, mean +/- s.d.) for an established human papillomavirus (hpv) lesion with or without concomitant cervical intraepithelial neoplasia (cin) were analysed for occurrence of langerhans cells, defined by the monoclonal antibody okt-6 using the avidin-biotin peroxidase complex (abc) technique. okt-6+ cells remained at the constant low level (1.5-1.9% of the inflammatory cells) i ...	1984	6151923
association of human papillomavirus subtype and clinical course in respiratory papillomatosis.	the relationship of human papillomavirus type 6 (hpv-6) subtypes to the clinical manifestations of respiratory papillomatosis was investigated. dna was isolated from biopsy specimens of 21 patients and the viral genome analyzed by molecular hybridization. four subtypes, designated hpv-6c through hpv-6f, were distinguishable by restriction endonuclease cleavage patterns of the viral genome. patient records were reviewed to identify associations between viral subtype and sex, race, age at onset of ...	1984	6318005
human papillomavirus infection and cervical intraepithelial neoplasia: histopathology and dna content.	to evaluate the reliability of diagnostic criteria for separating intraepithelial squamous lesions into low- and high-risk categories, 25 lesions of the cervix were diagnosed as flat condyloma, atypical immature metaplasia, or cervical intra-epithelial neoplasia with koilocytosis based on well-defined histologic criteria. the presumption was that flat condyloma and atypical immature metaplasia would be diploid/polyploid as compared to low-grade cervical intraepithelial neoplasia, which should be ...	1984	6318171
human papillomavirus infection of the uterine cervix: histological appearances in 28 cases identified by immunohistochemical techniques.	twenty eight biopsy specimens of the cervix showed positive immunohistochemical staining when treated with an antiserum raised against an internal capsid antigen of human papillomavirus (hpv). histological examination of adjoining sections from the same blocks showed a much wider range of abnormalities than those already described in association with hpv infection. the picture was usually diagnostic. it rested chiefly on identifying the koilocyte--the cell with the perinuclear halo that carries ...	1984	6319459
bowenoid papulosis. demonstration of human papillomavirus (hpv) with anti-hpv immune serum.	two cases of bowenoid papulosis (bp) occurred. the presence of human papillomavirus was demonstrated in one patient. in the other patient, condyloma acuminatum developed after initial spontaneous improvement in his lesions. the discovery of a possible oncogenic virus in bp and the epidemiologic behavior of carcinoma in situ and multicentric carcinomas suggest the need for a very careful follow-up evaluation of such lesions. bowenoid papulosis may be a chronic dysplasia with a variable long-term ...	1984	6322703
presence of human papillomavirus in verrucous carcinoma (ackerman) of the vagina. immunocytochemical, ultrastructural, and dna hybridization studies.	human papillomavirus (hpv) genomes were identified in two cases of verrucous carcinoma of the vagina, using southern blot dna hybridization under low-stringency conditions. type (group) 6 hpv dna (hpv-6) was identified, using molecularly cloned hpv-1 through hpv-6 dna probes under high-stringency conditions in both cases. in addition, dna extract in one case hybridized with hpv-1, hpv-3, and hpv-4 dna probes. no hpv structural proteins were demonstrated in either case by immunocytochemical tests ...	1984	6329128
papillomavirus infection of the cervix. iii: relationship of the presence of viral structural proteins to the expression of involucrin.	forty-two cervical biopsies with cervical intraepithelial neoplasia were compared with respect to the expression of human papillomavirus (hpv) structural proteins and the expression of the cellular structural protein involucrin, a marker of suprabasal squamous differentiation. hpv structural protein and involucrin expression displayed an inverse correlation with the severity of dysplasia. both of these proteins were detected in 11 of 28 cases (39%) of mild and moderate dysplasia, but in only two ...	1984	6329974
papillomavirus genomes: sequences and consequences.	the complete nucleotide sequences of four papillomavirus genomes recently determined (human papillomavirus types 1a and 6b, bovine papillomavirus type 1, and the shope cottontail rabbit papillomavirus) shed light on the molecular genetics of these epitheliotropic viruses. the organization of the four genomes is very similar, and their comparative analysis suggests new hypotheses about the nature of the viral proteins, their involvement in virus structure, and their interaction with the host cell ...	1984	6330226
hsv, cmv, and hpv in human neoplasia.	we are studying the role of sexually transmitted viruses in the development of human tumors. the persistence of herpes simplex virus, cytomegalovirus, and human papillomavirus nucleic acid sequences has been examined using cloned viral dna sequences as probes. the relationship of the viruses to various stages in the progression of neoplasia is examined, with particular reference to the role of viral and/or cellular genes in the initiation, promotion, and maintenance of the neoplastic phenotype. ...	1984	6330227
the pathology of superficially invasive, thin vulvar squamous cell carcinoma.	thirty-six cases of vulvar squamous cell carcinoma 5 mm or less in thickness were studied, and potential predictors of lymph node metastases were evaluated. tumor thickness and depth of stromal invasion were measured. inguinal lymph node metastases were present in six (17%) cases, all of which had primary neoplasms more than 3 mm thick. the most superficial lesion to have lymph node metastasis was 3.2 mm thick and had 1.6 mm of stromal invasion. nonetheless, depth of stromal invasion of less tha ...	1984	6511160
[focal epithelial hyperplasia in lepromatous leprosy].	focal epithelial hyperplasia heck (feh) is most likely caused by human papilloma virus. it mainly occurs in children and young people showing no associated diseases. for the first time, we describe a case of feh in a patient with lepromatous leprosy who due to persistent erythema nodosum leprosum has been treated with a lang-term glucocorticoid therapy. the question of the competence of lepromatous patients in resisting certain viral infections arises.	1983	6649746
human papilloma virus infection (condyloma) of the cervix and cervical intraepithelial neoplasia: a histopathologic and statistical analysis.	two hundred fifty consecutive cases previously diagnosed as advanced intraepithelial neoplasia (cin 2 and cin iii) were reviewed and examined for the presence of coexisting condyloma. when the mean ages and age distributions were analyzed, there was a consistent shift of the age distribution to a younger age among women with cin and condyloma as opposed to cin alone. furthermore, when the relationship between age and case accumulation was normalized from a curvilinear to a linear relationship, t ...	1983	6822369
effect of oral aromatic retinoid (ro 10-9359) on human papilloma virus-2-induced common warts.	persisting human papilloma virus (hpv) 2 induced common warts of a chronic lymphatic leukemia patient were orally treated with aromatic retinoid ro 10-9359 (tigason). clinically, the lesions improved rapidly. virus-specific cytopathogenic effects (cpe), virus particles and viral dna were no longer detectable. therapy was discontinued because of the development of a liposarcoma, which led to a complete relapse of the cutaneous lesions. hpv-2 specific parameters, cpe and viral dna, were restored. ...	1983	6840376
[false forms of bowen disease of the genital mucosa].	this review deals with the lesions of the genitalia histologically mimicking bowen's disease, yet behaving like a benign process. the report is based on data from the literature and on 14 personal cases, four of which are described here as examples. some of the cases, mostly young patients, showed spontaneous regression; in others condylomata acuminata developed on the site of the lesions; in rare instances a "transformation" in lichen planus occurred. for these not really precancerous condition ...	1983	6874339
identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes.	the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ...	1983	6313534
the story of a cell. the george n. papanicolaou award lecture.	the history of one cell type observed on gynecologic cytologic samples and later identified as pathognomonic of human papillomavirus (hpv) infection is presented. mention is also made of the role of hpv in the development of premalignant and malignant squamous lesions of the uterine cervix.	1983	6316699
human papillomavirus: detection of viral dna sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix.	colposcopic-directed biopsies of the uterine cervix from 22 patients were analyzed for the presence of human papillomavirus (hpv) dna and structural antigens. 11 of the biopsies were classified microscopically as mild dysplasia, 3 as moderate dysplasia, 1 as severe dysplasia, and 7 as squamous metaplasia. nonstringent hybridization with a bovine papillomavirus type 1 dna probe and immunocytochemical analysis with an antiserum against papillomavirus genus-specific structural antigens were perform ...	1983	6317605