Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| components required for in vitro cleavage and polyadenylation of eukaryotic mrna. | we have studied in vitro cleavage/polyadenylation of precursor rna containing herpes simplex virus type 2 poly a site sequences and have analyzed four rna/protein complexes which form during in vitro reactions. two complexes, a and b, form extremely rapidly and are then progressively replaced by a third complex, c which is produced following cleavage and polyadenylation of precursor rna. substitution of atp with cordycepin triphosphate prevents polyadenylation and the formation of complex c howe ... | 1988 | 2898767 |
| vaccine induced hsv 1 antibodies fail to correlate with protection against hsv 2 in guinea pigs. | guinea pigs immunised with hsv 1 subunit vaccine were bled, and subsequently challenged intravaginally with hsv 2. the clinical response to virus challenge was quantified, and correlations were sought between clinical score and virus-specific serum antibody titre in functional and binding assays (elisa, neutralization, complement-mediated cytotoxicity and antibody-dependent cellular cytotoxicity). no significant relationships were found, and it was concluded that reactivity in the serological as ... | 1988 | 2856207 |
| [herpes simplex virus type 2 antibody levels in sera from various age groups using elisa and ifat]. | we have investigated hsv-2, igg antibody levels in 180 sera which were obtained from 60 girl students between 18-20 years old, 60 pregnant women between 19-39 years old and 60 young men between 20-22 years old, by using elisa (enzyme linked immunosorbent assay) and ifat (indirect fluorescent antibody technique). all of the sera obtained from girl students and pregnant women were found negative for hsv-2, igg antibody by elisa. however 2 of 60 young men's sera were positive. totally 2 of 180 sera ... | 1988 | 2856217 |
| remyelination of central nervous system lesions in experimental genital herpes simplex virus infection. | to study spinal cord remyelination in a model of genital herpes simplex virus type 2 (hsv-2) infection, adult female mice were inoculated by a vaginal route. at intervals up to 6 months after infection, cord tissues were removed and examined by light and electron microscopy and by immunohistochemical methods. as a consequence of acute infection, 60% of mice developed multifocal central nervous system (cns) demyelinative lesions in the lower thoracic, lumbar, or upper sacral cord. these lesions, ... | 1988 | 3171599 |
| herpes-simplex-related antigen in human demyelinative disease and encephalitis. | using immunohistochemical methods optimized to detect herpes simplex virus type 2 (hsv-2) antigen, paraffin sections from human central nervous system tissues from 31 cases pathologically diagnosed as multiple sclerosis (ms), 34 cases of other neurological diseases, 4 adult cases of hsv encephalitis, and mouse brains infected with various hsv strains were examined. two distinct patterns of immunoreactivity with hsv antisera were seen. in typical acute human and experimental encephalitis, antigen ... | 1988 | 3176900 |
| comparative study of various immunomodulators for macrophage and natural killer cell activation and antiviral efficacy against exotic rna viruses. | several immunomodulators were compared for immunomodulatory and antiviral activity in b6c3f1 female mice. our results demonstrate that murine recombinant gamma interferon (rifn-g), human recombinant alpha a/d interferon (rifn-a), ampligen (a polyribonucleotide) and cl246,738 modulate nonspecific immunity and are effective antiviral agents in vivo. administration of each of these agents 1 day before cell harvest induced high levels of splenic natural killer (nk) cell activity against yac-1 target ... | 1988 | 3182149 |
| expression of herpes simplex virus glycoprotein d on antigen presenting cells infected with vaccinia recombinants and protective immunity. | we studied the effect of the temporal regulation of herpes simplex virus (hsv) type 1 glycoprotein d (gd-1) expression in ia+ epidermal cells (ec) and macrophages on virus specific immunity and protection from hsv-2 challenge. gd-1 was expressed on the surface of cells infected with a vaccinia recombinant containing gd-1 under the control of an early vaccinia virus promoter (vp176). it was not expressed in cells infected with a recombinant (vp254) in which gd-1 is controlled by a late vaccinia v ... | 1988 | 3263886 |
| hemorrhagic cystitis due to herpes simplex virus as a marker of disseminated herpes infection. | herpetic cystitis has rarely been reported. herein are described two immunocompromised female patients with genital or buttock herpetic lesions in whom hemorrhagic cystitis due to herpes simplex virus type 2 developed in the setting of disseminated herpetic infection. both patients had indwelling foley catheters. urinary tract instrumentation may cause local or widespread dissemination of genital herpes in immunocompromised patients. | 1988 | 3348273 |
| antiherpesvirus activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl) guanine (brl 39123) in animals. | the antiviral activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (brl 39123) was assessed in several animal models of herpes simplex virus (hsv) infection. brl 39123 was as active as acyclovir (acv) when applied topically to guinea pigs with a cutaneous hsv type 1 (hsv-1) infection and was also active topically in an hsv-2 genital infection. before systemic administration to infected animals, brl 39123 and acv were administered orally and subcutaneously to mice, and the blood was assayed f ... | 1988 | 3364954 |
| intravenous acyclovir therapy of first episodes of genital herpes: a multicenter double-blind, placebo-controlled trial. | a collaborative multicenter double-blind, placebo-controlled trial of intravenous acyclovir treatment of first-episode genital herpes was performed in order to substantiate previous findings on the efficacy and safety of this drug, to evaluate the influence of parenteral therapy on recurrence frequency, and to obtain further data on the natural history of genital herpes. | 1988 | 3046350 |
| the association between genital ulcer disease and acquisition of hiv infection in homosexual men. | we analyzed the association of herpes simplex virus (hsv) infection and syphilis, the two most common causes of genital ulceration in homosexual men, with human immunodeficiency virus (hiv) infection in 200 men enrolled between 1983 and 1986 into a study evaluating the microbial causes of acute proctitis. infection with hiv was independently associated with a history of syphilis, serologic evidence of syphilis, a history of hsv infection, and antibody to hsv-2. antibody to hiv was not associated ... | 1988 | 3404600 |
| cyclopentenylcytosine. a carbocyclic nucleoside with antitumor and antiviral properties. | cyclopentenylcytosine (cpe-c, 2), a pyrimidine analogue of the fermentation derived carbocyclic nucleoside neplanocin a, has been synthesized from the optically active cyclopentenylamine 3b by two synthetic routes. cpe-c demonstrates significant antitumor activity against both the sensitive and ara-c resistant lines of l1210 leukemia in vivo. multiple long term survivors are produced in both tumor models. the compound also gives 100% growth inhibition of the solid human a549 lung and mx-1 mammar ... | 1988 | 3411597 |
| induction of fc and c3b receptors on rabbit corneal cells by herpes simplex virus. | the expression of receptors for fc portion (fcr) of immunoglobulin g (igg) and for a c3b component of complement (c3br) by herpes simplex virus (hsv) was studied in primary cultures of rabbit corneal cells. monolayer cultures of epithelial, stromal and endothelial cells of the rabbit cornea were infected with three strains each of type 1 (hsv-1) and type 2 hsv (hsv-2). rosette methods were used to detect receptors by means of sheep erythrocytes sensitized with rabbit igg for fcr and c3b-coated s ... | 1988 | 3417420 |
| synthesis and antiviral activity of novel n-substituted derivatives of acyclovir. | novel n-substituted derivatives of acyclovir (1a) were synthesized and evaluated for their antiviral, antimetabolic, and antitumor cell properties in vitro. monomethylation of 1a at positions 1, 7, and n-2 gave compounds 2-4, respectively. when positions 1 and n-2 were linked together by an isopropeno group, the tricyclic 9-[(2-hydroxyethoxy)methyl]-1,n-2-isopropenoguanine (5) was obtained. compound 5 was then further methylated at positions n-2 and 7 to give 6 and 7, respectively. none of the n ... | 1988 | 2455050 |
| enhanced efficacy of nucleoside analogs and recombinant alpha interferon in weanling mice lethally infected with herpes simplex virus type 2. | weanling icr swiss mice were inoculated intranasally with a lethal dose of herpes simplex virus type 2, and treated with either vidarabine, vidarabine-5'-monophosphate, acyclovir or a hybrid recombinant human alpha interferon which is active in murine tissues. treatment with antiviral drugs was initiated 2, 24, 48, 72 or 96 h following virus inoculation. single drug treatment showed little effect on mortality, with only acyclovir showing some slight reduction. four dual drug combinations (vidara ... | 1988 | 2455473 |
| prior herpes simplex virus type 2 infection as a risk factor for hiv infection. | antibodies to cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex viruses types 1 and 2 (hsv-1, hsv-2) in three sequential serum samples from 62 men who did and 61 men who did not develop human immunodeficiency virus (hiv) infection at the time of the final (third) serum specimen were studied. antibody titers to cmv, ebv, and hsv-1 did not significantly rise in or differ between men who did or did not get hiv infection. however, we found that 32 (68%) of 47 hiv seroconverters had ... | 1988 | 2828700 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes. | we investigated the possibility that herpes simplex virus (hsv) glycoproteins might provide potential immunotherapy for recurrent hsv disease by using the guinea pig model of genital hsv infection. in two experiments, after recovery from initial genital herpes, 58 animals were randomized either to receive a glycoprotein vaccine or to serve as controls. both a mixture of hsv-2 glycoproteins derived from infected tissue cultures and a mixture of hsv-1 glycoproteins b and d, prepared by genetic eng ... | 1988 | 2826603 |
| regulation of glycoprotein d synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively? | earlier studies have described the alpha 4/c113 baby hamster kidney cell line which constitutively expresses the alpha 4 protein, the major regulatory protein of herpes simplex virus 1 (hsv-1). introduction of the hsv-1 glycoprotein b (gb) gene, regulated as a gamma 1 gene, into these cells yielded a cell line which constitutively expressed both the alpha 4 and gamma 1 gb genes. the expression of the gb gene was dependent on the presence of functional alpha 4 protein. in this article we report t ... | 1988 | 2824843 |
| entry of herpes simplex virus 1 in bj cells that constitutively express viral glycoprotein d is by endocytosis and results in degradation of the virus. | the bj cell line which constitutively expresses herpes simplex virus 1 glycoprotein d is resistant to infection with herpes simplex viruses. analysis of clonal lines indicated that resistance to superinfecting virus correlates with the expression of glycoprotein d. resistance was not due to a failure of attachment to cells, since the superinfecting virus absorbed to the bj cells. electron microscopic studies showed that the virions are juxtaposed to coated pits and are then taken up into endocyt ... | 1988 | 2824844 |
| a human cytomegalovirus function inhibits replication of herpes simplex virus. | human embryonic lung (hel) cells infected with human cytomegalovirus (hcmv) restricted the replication of herpes simplex virus type 1 (hsv-1). a delay in hsv replication of 15 h as well as a consistent, almost 3 log inhibition of hsv replication in hcmv-infected cell cultures harvested 24 to 72 h after superinfection were observed compared with controls infected with hsv alone. treatment of hcmv-infected hel cells with cycloheximide (100 micrograms/ml) for 3 or 24 h, conditions known to result i ... | 1988 | 2824846 |
| type-specific antibodies to herpes simplex virus type 2 (hsv-2) glycoprotein g in pregnant women, infants exposed to maternal hsv-2 infection at delivery, and infants with neonatal herpes. | we used a murine monoclonal antibody to herpes simplex virus (hsv) type 2 (hsv-2) glycoprotein g (gg) to develop an enzyme immunoassay that detected hsv-2 type-specific antibodies in human sera. antibodies to hsv-2 gg were detected in 98 (96%) of 102 sera from pregnant women with culture-proved hsv-2 infection. sixty-five percent of the women had serological evidence of past hsv-2 infection by the rawls index, based on titers of neutralizing antibody to hsv type 1 and hsv-2. thirty (88%) of 34 i ... | 1988 | 2826604 |
| enhanced in vitro reactivation of latent herpes simplex virus from neural and peripheral tissues with hexamethylenebisacetamide. | we evaluated the effect of the demethylating agent hexamethylenebisacetamide on reactivation of latent herpes simplex virus type 2 (hsv-2) from guinea pig neural and extraneural tissues. four explant cultures from the dorsal root ganglia of 42 latently infected guinea pigs and vaginal and cervical explant cultures from 33 animals were divided so that half received 5 mm of hexamethylenebisacetamide supplemented media and half media alone. hsv-2 was recovered earlier and from a greater percentage ... | 1988 | 2833203 |
| human papillomavirus, herpes simplex virus and cervical cancer incidence in greenland and denmark. a population-based cross-sectional study. | in greenland, the incidence of cervical cancer is 5.7 times higher than in denmark among women aged 20-39. from nuuk (greenland) and nykøbing falster (denmark) a sample of 800 women aged 20-39 years was drawn at random. a total of 586 and 661 women were investigated in greenland and denmark respectively. all women had a gynecological examination including a pap-smear and cervical scrape for hpv-analysis (filter in situ hybridization). a blood sample was taken for analysis of hsv type-specific an ... | 1988 | 2833450 |
| the epidemiology of infection with the human herpesviruses in navajo children. | investigations of serum antibody status to the five human herpesviruses--herpes simplex virus type 1, herpes simplex virus type 2, cytomegalovirus, epstein-barr virus, and varicella-zoster virus--were conducted on 197 navajo children, aged 1-15 years, at a reservation pediatric outpatient clinic in crownpoint, new mexico, from 1981-1984. to determine the severity of infection with the human herpesviruses, the authors reviewed lifetime medical records of 166 of the children for evidence of herpes ... | 1988 | 2833854 |
| generation of a herpes simplex virus type 2 variant devoid of xbai sites: removal of the 0.91 map coordinate site results in impaired synthesis of glycoprotein g-2. | herpes simplex virus (hsv) type 2 (strain hg52) has four xbai sites at map coordinates (m.c.) 0.45, 0.7, 0.91 and 0.94, i.e. two in the unique long and two in the unique short regions of the genome. previously, we had isolated a genome containing only the 0.45 and 0.94 xbai sites. here we report the isolation of a mutant (jh2611) in which all four xbai sites have been removed using an enrichment selection procedure, without any loss of viability. removal of each site has been shown to be due to ... | 1988 | 2826660 |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| efficacy of recombinant glycoprotein d subunit vaccines on the development of primary, recurrent, and latent genital infections with herpes simplex virus type 2 in guinea pigs. | vaccination of guinea pigs with recombinant glycoprotein d (subunit vaccine) from either herpes simplex virus type 1 or type 2 (hsv-1, hsv-2), before intravaginal inoculation with hsv-2, markedly altered viral replication in the vaginal tract and reduced, or completely prevented, the development and spread of external genital lesions. vaccination with glycoprotein d from hsv-2 prevented the establishment of latent hsv-2 infections in dorsal root ganglia and reduced the frequency of recurrent epi ... | 1988 | 2834467 |
| the effect of triterpenoid compounds on uninfected and herpes simplex virus-infected cells in culture. iii. ultrastructural study of virion maturation. | electron microscope studies been made of mock-infected or herpes simplex virus (hsv) type 1 (strain 17)- or hsv-2(strain hg52)-infected flow 2002 cells grown in the absence or presence of various concentrations of cicloxolone sodium (ccx). fifty non-serial, thin sections of mock-infected or hsv-infected cells, which contained a portion of the nucleus, were examined by transmission electron microscopy. with increasing drug concentration, counts of capsid structures both in the nucleus and cytopla ... | 1988 | 2828518 |
| synthesis and biological activities of 4-o-(difluoromethyl)-5-substituted-uracil nucleoside analogues. | various 4-o-difluoromethyl analogues of 5-substituted uridine (urd), 2'-deoxyuridine (durd), and arabinofuranosyluracil (arau) nucleosides were prepared via a cf2-insertion reaction into 4-o-silylated nucleosides and evaluated for activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and cytotoxicity in human embryonic lung fibroblast (helf) cell cultures. the introduction of the 4-substituent led to a strong reduction of antiviral activity for durd but not for arau analogues. ... | 1988 | 2828623 |
| herpes simplex virus virion host shutoff function. | herpes simplex virus (hsv) virions contain one or more functions which mediate the shutoff of host protein synthesis and the degradation of host mrna. hsv type 1 (hsv-1) mutants deficient in the virion shutoff of host protein synthesis (vhs mutants) were isolated and were found to be defective in their ability to degrade host mrna. furthermore, it was found that viral mrnas in cells infected with the vhs 1 mutant have a significantly longer functional half-life than viral mrnas in wild-type viru ... | 1988 | 2828686 |
| herpes simplex virus type 2 establishes latency in the mouse footpad. | balb/c mice were infected with herpes simplex virus type 2 by inoculation into the footpad. three months or more after infection, and in the total absence of any evidence of illness, latent virus could be reactivated from both ganglia and footpad tissue but only after explantation and culturing for a week or more. virus recovery from the footpad could not be prevented by treatment of the infected mice with acycloguanosine for up to 6 months in their drinking water at 40 mg/kg, nor by femoral and ... | 1988 | 2828516 |
| herpes simplex virus type 2 latency in the footpad of mice: effect of acycloguanosine on the recovery of virus. | herpes simplex virus type 2 has been reactivated from the latent state in the footpad and dorsal root ganglia of acycloguanosine-treated balb/c mice. virus was also recovered from the footpad tissue but not from the ganglia of denervated, latently infected mice. treatment in vitro of explanted footpad cultures with acycloguanosine or phosphonoacetic acid did not affect the rate of virus reactivation. in all the isolates examined the virus was found to be acycloguanosine-sensitive. recovery of vi ... | 1988 | 2828517 |
| insertion and extension of acyclic, dideoxy, and ara nucleotides by herpesviridae, human alpha and human beta polymerases. a unique inhibition mechanism for 9-(1,3-dihydroxy-2-propoxymethyl)guanine triphosphate. | the ability of human alpha and beta dna polymerases and herpes simplex virus type 2 (hsv-2) and human cytomegalovirus (hcmv) dna polymerases to insert and extend several nucleotide analogs has been investigated using a variation of sanger-coulson dna sequencing technology. the analogs included the triphosphates of two antiviral nucleosides with incomplete sugar rings: 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) and 9-(2-hydroxyethoxymethyl)guanine (acyg or acyclovir), as well as dideoxy and ... | 1988 | 2831212 |
| evidence that the gene for herpes simplex virus type 1 dna polymerase accounts for the capacity of an intertypic recombinant to spread from eye to central nervous system. | hsv-1(17) replicates 100-fold more efficiently than hsv-2(186) within trigeminal ganglia following ocular infection. in order to identify the nucleotide sequences responsible for the differences in the capacity of the two hsv strains to grow within the peripheral nervous system, an intertypic recombinant was generated by infecting neuroblastoma cells with hsv-2(186) and a hsv strain possessing nucleotide sequences from hsv-1(17). the genome of the intertypic recombinant was composed entirely of ... | 1988 | 2831653 |
| zwitterionic detergent solubilisation of hsv-1 surface antigens. | a preparation was obtained from herpes simplex virus type 1 (hsv-1)-infected cells using a zwitterionic detergent, empigen bb. the preparation was partially-purified either by ultracentrifugation over a cusion of 20% sucrose or on a sucrose density gradient. partial characterisation of these materials by elisa, using both polyclonal and monoclonal antibodies showed them to contain at least four major hsv glycoproteins, gb, gc, gd and ge. comparison of empigen-extracted hsv-1 antigen preparations ... | 1988 | 2831853 |
| genital virus infection and cervical neoplasia. | the reported association between genital infection by herpes simplex virus (hsv) type 2 and cervical neoplasia has been investigated at a molecular level. tissue culture of over 50 cervical cancers resulted in the development of several cell lines but none of these expressed hsv information. the permanent cell line, cx106, which was generated was found to contain multiple genome copies of human papillomavirus (hpv) type 16. southern blot analysis of nine cervical cancers showed an authentic 2.8 ... | 1988 | 2831934 |
| antiviral activity of chamaecyparis lawsoniana extract: study with herpes simplex virus type 2. | ethanolic extract of the green part of chamaecyparis lawsoniana was tested for antiviral activity and toxicity in tissue culture. all experiments were carried out in confluent human embryo lung fibroblasts. treatment of fibroblast cells with extracts after viral inoculation was effective in inhibiting the replication of herpes simplex virus type 2 (hsv-2). the critical time for virus inhibition was 4 to 5 h after virus absorption. the antiviral activity was assayed employing the techniques of vi ... | 1988 | 2832710 |
| use of routine viral cultures at delivery to identify neonates exposed to herpes simplex virus. | we obtained specimens for viral culture from mothers, infants, or both at the time of 6904 deliveries, without regard to the mothers' history of genital herpes. herpes simplex virus (hsv) was recovered in cultured specimens from 14 of the 6904 deliveries (0.20 percent); all 14 mothers were asymptomatic. all viral isolates were herpes simplex virus type 2 (hsv-2). only 1 of the 14 women (7 percent) had a history of genital herpes, whereas 12 (86 percent) had serologic evidence of a previous infec ... | 1988 | 2832756 |
| expression of the hsv-2 ribonucleotide reductase subunits in adenovirus vectors or stably transformed cells: restoration of enzymatic activity by reassociation of enzyme subunits in the absence of other hsv proteins. | we have cloned the large subunit (rr1) of the hsv-2 ribonucleotide reductase into a helper-independent adenovirus 5 vector under control of the viral major late promoter. infection of 293 cells with the adred-1 recombinant virus resulted in the expression of the hsv-2 rr1 protein. we have also produced cells which constitutively express the small (rr2) subunit of the hsv-2 enzyme by transfecting 293 cells with a plasmid encoding this protein and the neo resistance marker (psv2neo-rr2). infection ... | 1988 | 2833019 |
| effect of progesterone on the replication of herpes simplex virus type 2 in vitro. | this project involved an investigation into the effect of progesterone on the replication of herpes simplex virus type 2 in a vero-cell culture. the first part of the experiment relates to preincubation of the cells with progesterone in the duration of 48 hours. after innoculation, replication of the virus was continued in the presence of progesterone. samples for titration were taken 10, 21 and 32 hours after innoculation for the purpose of determining the single-cycle replication curve of herp ... | 1988 | 2834117 |
| effect of progesterone and pregnancy on the replication of herpes simplex virus type 2 in vivo. | this project involved an investigation into the effect of pregnancy and progesterone on genital herpes simplex virus (hsv) type 2 infection in mice, with the aim of establishing whether elevated progesterone level, due either to pregnancy or parenteral administration, makes the vaginal hsv infection all the heavier. the investigation was carried out on three groups of mice, representing three test situations in connection with vaginal hsv infection: the first group consisted of non-pregnant expe ... | 1988 | 2834118 |
| electron microscopic localization of acridine orange chromatin interaction products in cells transformed by herpes simplex virus type 2. | the purpose of this study was to examine the distribution pattern of acridine orange (ao) binding to dna in transformed cells and derived tumor cells. two cell clones from hamster embryonic fibroblasts (hef) transformed by herpes simplex virus type 2, tumor cells derived from the transformed cell clones, and normal hef in culture were processed for ultracytochemistry. uptake studies and autoradiography using [3h] thymidine and [3h]-uridine were also performed. ultrastructural examination reveale ... | 1988 | 2851256 |
| presence of insulin binding sites on viral particles. | using a standard radio-receptor assay, we have demonstrated that [125i]insulin can bind specifically to each of two types of purified enveloped viruses, influenza a virus and rous sarcoma virus. a non-enveloped icosahedral virus (echovirus 11) and herpes simplex virus type 2, which acquires its envelope from the nuclear membrane of the cell, did not possess insulin receptor activity. displacement of specifically bound radiolabelled insulin from the viral surface was achieved by addition of an ex ... | 1988 | 2851292 |
| the use of colloidal gold immunoelectron microscopy to diagnose varicella-zoster virus (vzv) infections by rapid discrimination between vzv, hsv-1 and hsv-2. | colloidal gold immunoelectron microscopy was used to diagnose rapidly 53 cases clinically suspected of varicella-zoster virus (vzv) infection and one special case selected from another study on typical herpes simplex virus (hsv) infections. the viruses were identified and subsequently typed within 2.5 h by a direct labelling test for vzv, and within 3.5 h by an indirect labelling test with monoclonal antibodies against hsv type 1 and type 2. the protein a purified igg fraction of human anti-vzv ... | 1988 | 2851604 |
| secretory iga against herpes simplex virus in cervical secretions. | a recently developed method to recover proteins from cervical secretions was combined with methods to detect minute concentrations of herpes simplex virus (hsv) type specific antibodies to measure the concentrations of locally produced antibodies in women with genital infections. forty nine women attending a sexually transmitted disease (std) clinic were included. cervical secretions were obtained by suction into a plastic catheter. soluble proteins were recovered from the secretions by elution ... | 1988 | 2852157 |
| broad-spectrum antiviral activity of the acyclic guanosine phosphonate (r,s)-hpmpg. | (r,s)-9-(3-hydroxy-2-phosphonomethoxypropyl)guanine [(r,s)-hpmpg] exhibits broad spectrum antiviral activity with an ed50 of less than 1 microm against herpes simplex virus (hsv) types 1 and 2, varicella zoster virus, human cytomegalovirus (hcmv) and vaccinia in plaque reduction assays. wild type hsv-2 and its thymidine kinase deficient variant are equally sensitive to (r,s)-hpmpg. (r,s)-hpmpg is 100-fold more potent than acyclovir (ed50 = 0.45 microm vs. 44 microm, respectively) against hcmv in ... | 1988 | 2852486 |
| [seroepidemiological survey of herpes simplex virus type 2 in cervical erosion women]. | 1988 | 2852541 | |
| herpes simplex virus induces the replication of foreign dna. | plasmids containing the simian virus 40 (sv40) dna replication origin and the large t gene are replicated efficiently in vero monkey cells but not in rabbit skin cells. efficient replication of the plasmids was observed in rabbit skin cells infected with herpes simplex virus type 1 (hsv-1) and hsv-2. the hsv-induced replication required the large t antigen and the sv40 replication origin. however, it produced concatemeric molecules resembling replicative intermediates of hsv dna and was sensitiv ... | 1988 | 2850486 |
| neurovirulence of individual plaque stocks of herpes simplex virus type 2 strain hg 52. | the virulence of ten independent plaque stocks of hsv-2 strain hg 52 was determined by intracranial inoculation of 3 week old balb/c mice. the ten stocks exhibited a range of ld50 which could be grouped into three classes; high (less than 10(3) pfu/mouse), intermediate (10(3)-10(4) pfu/mouse) and low (greater than 10(5) pfu/mouse). analysis of the ten stocks using six different restriction endonucleases showed no significant variation in the size and distribution of fragments. isolates from the ... | 1988 | 2850778 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes reduces cervicovaginal virus shedding in guinea pigs. | treatment of previously infected guinea pigs with herpes simplex virus (hsv) glycoproteins reduces the frequency and severity of subsequent genital recurrences. an effective vaccine should also reduce episodes of viral shedding. in this study, hsv glycoproteins b and d treatment of animals experiencing recurrent genital herpes reduced the frequency of both clinical recurrences and cervicovaginal viral shedding. when virus was shed, however, the peak viral titer and duration of shedding was unalt ... | 1988 | 2852922 |
| the aetiological relationship between herpes simplex virus type 2 and carcinoma of the cervix: an unanswered or unanswerable question? | after two decades of investigation the proposed aetiological link between herpes simplex virus type 2 (hsv2) and human carcinoma of the cervix remains unproved. the initial seroepidemiological studies, which were of a retrospective nature, were in favour of the aetiological relationship, whereas the more recent prospective studies suggest that hsv infection is a co-variable. the detection of hsv genes and their products in tumour tissues has been plagued by problems of cross reactivity, but the ... | 1988 | 2853997 |
| cervical carcinoma: an experimental approach. | a mouse model system was used to investigate the preventive efficacy of a subunit herpes simplex virus (hsv) vaccine on the development of hsv induced cervical carcinoma. ten groups of mice were vaccinated before receiving repeated intravaginal exposure to hsv-type 2 inactivated by ultraviolet irradiation. at 20 months postvaccination, neutralizing antibody activity to herpes simplex viruses was detected in the sera of the mice which had received the highest vaccine dose. although three experime ... | 1988 | 2853998 |
| dna sequence and rna transcription through a site of recombination in a non-neurovirulent herpes simplex virus intertypic recombinant. | re6 is a herpes simplex type-1 (hsv-1) x herpes simplex type-2 (hsv-2) intertypic recombinant that cannot replicate in the adult mouse nervous system. in the accompanying report, we have shown that hsv-1 sequences between 0.698 and 0.721 map units can restore a partial neurovirulent phenotype to re6. in this report, we have used comparative dna sequence analysis of re6, 17syn+ (hsv-1) and hg52 (hsv-2) to demonstrate that this region contains a site of recombination between hsv-1 and hsv-2 sequen ... | 1988 | 2854325 |
| antiviral activities of 2'-deoxyribofuranosyl and arabinofuranosyl analogs of sangivamycin against retro- and dna viruses. | eight sugar-modified pyrrolopyrimidine nucleoside analogs related to the antibiotic sangivamycin were evaluated in cell culture against herpes simplex types 1 (hsv-1) and 2 (hsv-2), cytomegalovirus (cmv), adenovirus, and visna virus. five of the compounds were highly active against most of the viruses with 50% inhibition (ed50) values of 1-10 microm. the selectivity of the agents was low, with inhibition of uninfected cell proliferation occurring within 5-fold that of the virus ed50 for most of ... | 1988 | 2854957 |
| antiherpes activity of the immunomodulator ok-432, a streptococcal preparation, in immunosuppressed mice. | the antiviral activity of ok-432, an antitumor agent originating from streptococcal preparations, against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). intraperitoneal administration of ok-432 to mice 1 day after treatment with 200 mg cy/kg prevented death due to hsv-2 encephalitis in a dose-dependent manner. when the immunosuppressed mice were given ok-432 prior to hsv-2 infection, both by the intraperitoneal route, virus growth in the p ... | 1988 | 2854959 |
| human papillomavirus and herpes simplex virus in vulvar squamous cell carcinoma in situ. | human papillomavirus deoxyribonucleic acid were detected in tissue specimens from 38 to 46 patients (83%) with squamous cell carcinoma in situ of the vulva. herpes simplex virus type 2--related antigen icsp 34/35 was demonstrated in 23 of the lesions (50%), and antibodies to herpes simplex virus types 1 and 2 were found in 74% and 65% of the serum samples tested, respectively. both human papillomavirus deoxyribonucleic acid and herpes simplex virus type 2 antigen were detected in 19 cases (41%). ... | 1988 | 2834955 |
| comparison of western blot (immunoblot) and glycoprotein g-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera. | sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (hsv-1)- and hsv-2-specific antibodies by both a western blot (immunoblot) technique (wba) and immunodot enzyme assays (ieas) specific for hsv-1 or hsv-2 glycoprotein g (gg). of 137 serum samples tested, none was mistyped by either wba or iea. both tests were most sensitive with sera obtained at least 21 days after onset of primary hsv-2 infections or sera drawn during recurrent hsv-2 gen ... | 1988 | 2835389 |
| differential effect of systemic acyclovir treatment of genital hsv-2 infections on antibody responses to individual hsv-2 proteins. | western blot and reflectance densitometry were used to evaluate the antibody response from patients treated with systemic acyclovir during their primary episodes of genital hsv-2 infection. of 39 patients studied, 10 received oral acyclovir, 10 received intravenous acyclovir, and 19 received placebo. total antibody levels as well as levels of antibodies to individual hsv-2 proteins (gb, gg, gc/ge, vp16, gd, and p45) were determined in convalescent phase sera. the median number of hsv-2 proteins ... | 1988 | 2835427 |
| phospholipid-sensitive, ca2+-dependent protein kinase activity in rat embryo fibroblasts transformed by herpes simplex virus type 2. | increased cytosolic phospholipid-sensitive, ca2+-dependent protein kinase c (pk-c) activity is correlated with the highly tumorigenic potential of rat embryo fibroblasts transformed by herpes simplex virus type 2 (hsv-2). treatment of the cells with 12-o-tetradecanoylphorbol-13-acetate (tpa) caused a decrease in the cytosolic pk-c with a concomitant increase in pk-c recovered in the membrane fraction. translocation of the pk-c was dependent upon length of exposure to the phorbol diester. pk-c ac ... | 1988 | 2835720 |
| suppression of humoral immune response against herpes simplex virus induced by defective strains, ts- and tk- mutants. | suppression of humoral antibody formation against hsv is not only induced by replicating herpes simplex virus type 2 (hsv-2) but also by the defective strain ang and the deletion mutant 1301 of herpes simplex virus type 1 (hsv-1). moreover, ts-mutants a, h, k, s, 1201 and 1208 of hsv-1 as well as some ts-mutants of hsv-2 and "defective-interfering" particles of hsv-1 after high multiplicity of infection-passages induced suppression. treatment of infected mice with acg reduced antibody-formation ... | 1988 | 2835944 |
| anal cancer in women. | we studied predisposing factors in 56 women with anal cancer, comparing them with 56 matched controls drawn from the population. a detailed pretested questionnaire was administered to each study subject in a structured interview and blood was drawn for detection of herpes simplex virus antibodies by radioimmunoassay. pathologic material from cases was obtained and evidence of human papilloma virus infection was sought. by univariate analyses we found associations between anal cancer and positive ... | 1988 | 2836255 |
| a test for the relative potency of herpes simplex virus vaccines based upon the female guinea-pig model of hsv 2 genital infection. | an elisa for total herpes simplex virus (hsv) 1 antigen content and a test of immunogenicity based upon the female guinea-pig model of hsv 2 genital infection were applied to two samples from batches of hsv 1 subunit ('skinner') vaccine. the elisa was reproducible within an approximately threefold limit of error and batches 1 and 2 were indistinguishable in antigen content. the effects of vaccination in the guinea-pig model were assessed by a statistical analysis of scores derived from the princ ... | 1988 | 2836425 |
| transmission of genital herpes in couples with one symptomatic and one asymptomatic partner: a prospective study. | to determine the risk of transmission of genital herpes simplex virus (hsv) infection, we prospectively studied, for a median of six months, 38 couples who had been together for a median of 10 mo. in each couple, one partner had a history of symptomatic genital herpes and one did not. at entry, of the 38 asymptomatic, exposed partners, 21 were seronegative, and results of western blot analysis showed that seven had antibody to hsv type 1 (hsv-1), four to hsv type 2 (hsv-2), and six to both hsv-1 ... | 1988 | 2836518 |
| antibody to herpes simplex virus (hsv) icp-35 proteins after hsv challenge of animals immunized with hsv subunit vaccines. | the serological response to the icp-35 group of herpes simplex virus type 2 (hsv-2) proteins was examined 30 d after vaginal challenge with hsv-2 of guinea pigs previously immunized with hsv glycoproteins. detection of antibodies to icp-35 proteins correlated best with the magnitude of vaginal viral replication. animals that developed antibody to icp-35 proteins had more-frequent recurrences (p less than .0002) that were of longer duration (p less than .005) than animals that did not. by covaria ... | 1988 | 2836519 |
| antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of uv b-irradiated mice. | uv b-irradiation (280 to 320 nm) of mice at the site of cutaneous infection with herpes simplex virus type 2 (hsv-2) induced suppressor t-cell circuits that decreased hsv-2-induced proliferative responses of hsv-2-immune lymph node cells. adoptive transfer experiments indicated that splenocytes from uv b-irradiated hsv-2-infected animals contain l3t4+ cells that suppress proliferative responses in vivo, consistent with suppressor inducer cells. however, following in vitro culture of the splenocy ... | 1988 | 2836632 |
| placebo-controlled study with subunit herpes simplex virus vaccine in subjects suffering from frequent herpetic recurrences. | the safety and efficacy of a subunit herpes simplex virus (hsv) type 1 vaccine were tested in a small-scale double-blind trial carried out in a group of 42 volunteers suffering from frequent recurrences of herpetic lesions. the patients were paired according to sex, age, type of virus isolated, previous history of the disease and some non-specific immunological markers. one member of each pair received repeated doses of hsv vaccine, the other a placebo. clinical reactions were mild. antibody res ... | 1988 | 2844031 |
| monoclonal antibodies directed against a 130k glycoprotein of bovine herpesvirus 2 cross-react with glycoprotein b of herpes simplex virus. | envelope proteins of bovine herpesvirus type 2 (bhv-2) and herpes simplex virus types 1 and 2 (hsv-1, -2) share cross-reacting determinants. monoclonal antibodies directed against epitopes of a 130k glycoprotein of bhv-2 detect determinants on the surface of infected cells and react either with cytoplasmic or nuclear antigens. biological and biochemical characterization of the proteins recognized by these antibodies revealed that the bhv-2 specific 130k glycoprotein and the gb of hsv share commo ... | 1988 | 2837013 |
| incorporation into nucleic acids of the antiherpes guanosine analog buciclovir, and effects on dna and protein synthesis. | using cells expressing herpes simplex virus (hsv) thymidine kinase, we investigated the metabolism of the acyclic antiherpes guanosine analog buciclovir, in relation to the effects of the drug on viral dna and protein synthesis. in these cells the predominant metabolite of buciclovir was its triphosphate, as in the hsv-1 infected vero cells investigated in parallel. further metabolism of buciclovir led to incorporation into rna and dna. buciclovir inhibited dna synthesis, not rna synthesis, and ... | 1988 | 2837218 |
| prevalence of herpes simplex virus type 2 antibodies in selected population groups in italy. | the estimated prevalence of herpes simplex virus type-2 specific antibodies was less then 0.1% in a national sample of 1,169 healthy young males, 4.8% among 411 health care workers and 55% among 397 homosexuals. these viral infections seem to pose a health problem for risk groups such as homosexuals but not for the general population. | 1988 | 2837392 |
| class-specific herpes simplex virus antibodies in sera and cervical secretions from patients with cervical neoplasia: a multi-group comparison. | serum and cervical secretions were collected from patients with cervical dysplasia, carcinoma-in-situ (cis), squamous cell carcinoma (cervical scc), and controls with normal cervices, attending clinics within the west lambeth health district, london. enzyme-linked immunosorbent assays were used to examine cervical secretory iga (siga) and serum igg and iga antibodies to herpes simplex virus (hsv). sexual and demographic factors were considered during data analysis, which involved fitting multipl ... | 1988 | 2837405 |
| hypomethylation of host cell dna synthesized after infection or transformation of cells by herpes simplex virus. | infection of rat embryo cells with herpes simplex virus type 2 caused undermethylation of host cell dna synthesized during infection. dna made prior to infection was not demethylated, but some of its degradation products, including methyl dcmp, were incorporated into viral dna. the use of mutant virus showed that some viral dna synthesis appears to be required for the inhibition of methylation. inhibition of methylation cannot be explained by an absence of dna methyltransferase as the activity o ... | 1988 | 2837642 |
| [prevention of lymphatic and hematologic spreading as a pathogenic condition for neural persistence of herpes simplex virus]. | in the experimental model of genital herpes simplex virus (hsv) infection of the mouse--in spite of abundant virus replication on the mucous membranes--no infectious virus can be isolated from the inflamed and swollen draining lymph nodes (dln), contrary to the positive results in the lumbosacral nerves and their associated ganglia. attempts to reactivate an abortive infection possibly established in lymph node or spleen cells by stimulation with phytohemagglutinin or lipopolysaccharide rendered ... | 1988 | 2837877 |
| regulation of viral and cellular genes in a human neuroblastoma cell line latently infected with herpes simplex virus type 2. | a latent state of the herpes simplex virus type 2 genome was established in a human neuroblastoma cell line (sms-kcnr) to initiate studies on the mechanism by which host cells interact and regulate latent viral genes. to establish viral latency, it was necessary to prevent virus replication by briefly exposing the infected cells to antiherpetic acycloguanosine (20 microm) and human interferon (120 u/ml). subsequently however, these cells could be propagated without any antiherpetic agents and al ... | 1988 | 2838126 |
| interaction of polylysine with the cellular receptor for herpes simplex virus type 1. | we earlier reported that neomycin blocked reversibly the binding of herpes simplex virus type 1 (hsv-1) to the receptor of bhk cells, while the binding of hsv-2 to the receptor was unaffected. we could not determine whether the effect was on the virus particle, the receptor, or both. we have now tested several other cationic substances, and report that polylysine (and polyarginine) block the binding of hsv-1 to the receptor by interfering with the cellular receptor function; higher molecular wei ... | 1988 | 2838567 |
| effect of herpes simplex virus type 2 infection on mitochondrial gene expression. | a cdna clone derived from the mitochondrial cytochrome oxidase i gene has been used to show that the level of mitochondrially encoded rna species declines during herpes simplex virus type 2 infection in a manner similar to that for rna species derived from nuclear genes. in contrast to the situation for nuclear genes, however, no change in the transcription rate of the mitochondrial genome during infection was detected, indicating that post-transcriptional processes alone are responsible for the ... | 1988 | 2838578 |
| inhibition of herpes simplex virus type 2 replication in vitro by 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a. | the in vitro antiviral activity as well as the mechanism of action of a new antiviral agent, a kanamycin analogue, 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a (ptka) against herpes simplex virus type 2 (hsv-2) was investigated. the drug showed excellent antiviral action with negligible cytotoxic effect on the culture cells. based on plaque reduction assays the 50% inhibitory dose (id50) of the drug was 1 microgram/ml, and at 20 micrograms/ml plaque formation was totally suppressed. the c ... | 1988 | 2839107 |
| purification of the herpes simplex virus type 1 65-kilodalton dna-binding protein: properties of the protein and evidence of its association with the virus-encoded dna polymerase. | using a combination of conventional column chromatography and velocity sedimentation, we have purified the 65-kilodalton dna-binding protein (65kdbp) encoded by herpes simplex virus (hsv) greater than 625-fold. the hsv type 1 (hsv-1)-encoded dna polymerase (pol) cofractionated with 65kdbp through deae-sephacel, blue sepharose, and mono q columns and was only separated from 65kdbp by sedimentation through a glycerol gradient. immunoaffinity columns containing monoclonal antibody (mab) 6898 immuno ... | 1988 | 2839706 |
| changing patterns of genital herpes simplex virus infections. | herpes simplex virus type 2 is considered by many venereologists as the predominant serotype among those patients suffering from genital herpes. however, identification of genital isolates obtained from 16 through 20, and 31 through 40 year old individuals among our female patient population shows a predominance of hsv-1 infections. these data suggest that the "above the waist"-"below the waist" epidemiologic patterns commonly ascribed to reflect a prevalence of hsv-1 and hsv-2 infections respec ... | 1988 | 2839940 |
| methyl gallate, methyl-3,4,5-trihydroxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. i. purification and characterization of methyl gallate from sapium sebiferum. | a potent anti-herpetic compound was identified and purified to homogeneity from the leaves of sapium sebiferum by plaque reduction assay using herpes simplex virus type 2. the chemical structure of the purified compound was determined by mass spectroscopy and proton and carbon-13 nuclear magnetic resonance as methyl gallate, methyl-3,4,5-trihydroxybenzoate. this is the first demonstration that methyl gallate is a potent anti-herpetic compound in vitro, present in high concentration in the leaves ... | 1988 | 2840132 |
| methyl gallate, methyl-3,4,5-trihydoxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. ii. antiviral activity of methyl gallate and its derivatives. | methyl gallate (mg), methyl-3,4,5-trihydroxybenzoate, was highly active against herpes viruses as determined by plaque reduction assay. herpes simplex virus type 2, ms strain, was sensitive to mg at a mean 50% inhibitory concentration (ic50) of 0.224 micrograms/ml in monkey kidney cells. mg was specific for herpes viruses with the relative sensitivity hsv-2 greater than hsv-1 greater than cmv. two rna viruses tested were significantly less sensitive to mg. the structural components of mg which m ... | 1988 | 2840133 |
| detection of herpes simplex virus type 2 dna in uterine cervix lesions using cloned bgl ii n fragment of hsv-2 dna as a probe. | 1988 | 2840390 | |
| effect of macrophage activation on resistance of mouse peritoneal macrophages to infection with herpes simplex virus types 1 and 2. | to define the effect of heterogeneity of murine peritoneal macrophages (m phi) on intrinsic resistance to herpes simplex virus (hsv) infection, several m phi populations were characterized for their response to infection with hsv type 1 (hsv-1) and hsv-2. steady-state resident m phi (res m phi) were compared in parallel with m phi activated with corynebacterium parvum (now designated propionibacterium acnes) (cp m phi) and thioglycollate-elicited inflammatory m phi (tg m phi). res m phi were com ... | 1988 | 2841412 |
| specific induction of cellular gene transcription in herpes simplex virus type 2-transformed cells. | in spite of much work, the mechanism of oncogenic transformation by herpes simplex virus (hsv) is as yet unknown. it has been proposed that hsv type 2 (hsv-2) can transform cells by a 'hit and run' mechanism. in the past we have demonstrated that several polypeptides can be immunoprecipitated from hsv-2-transformed cells, but not from control cells or adenovirus-transformed cells, by rabbit hyperimmune sera to hsv-2. it is possible that the expression of these proteins might be the result of act ... | 1988 | 2841413 |
| a cellular protein related to heat-shock protein 90 accumulates during herpes simplex virus infection and is overexpressed in transformed cells. | a monoclonal antibody produced from mice immunized with hsv-infected cell dna binding proteins reacts with two cell-encoded polypeptides, p90 and p40, synthesized constitutively by many different cell types and expressed at high levels in transformed cells. in uninfected cells heat shock induces a cytoplasmic accumulation of p90 and in agreement with this we show that p90 is related to hsp90. during hsv-2 lytic productive infection, p90 accumulates to very high levels, such accumulation being de ... | 1988 | 2842174 |
| the cleavage recognition signal is contained within sequences surrounding an a-a junction in herpes simplex virus dna. | herpesvirus genome maturation involves site-specific cleavage of viral dna concatemers and encapsidation of unit-length molecules, processes that are apparently coupled. here, applying a transfection-infection approach, we have investigated the arrangement of the dna sequence elements involved in cleavage and shown that specific cleavage occurs independently of dna replication. we show that the cis-acting signal for cleavage is located within a 179-bp fragment from across an a-a junction formed ... | 1988 | 2847414 |
| in vivo behavior of genetically engineered herpes simplex viruses r7017 and r7020: construction and evaluation in rodents. | the herpes simplex virus (hsv) recombinant r7017 was constructed from hsv-1 (strain f) by deleting a portion of the thymidine kinase (tk) gene and by replacing the sequences representing the internal inverted repeats and adjacent genes in the l component with a fragment of the hsv-2 genome encoding the glycoproteins g, d, i, and a portion of e. in addition, the r7020 recombinant contains an hsv-1 dna fragment encoding the tk gene fused to the alpha 4 gene promoter. the results of studies in mice ... | 1988 | 2842408 |
| history of recurrent mucocutaneous herpes correlates with relatively low interferon production by herpes simplex virus-exposed cultured monocytes. | monocytes separated from blood specimens of healthy voluntary blood donors were infected with herpes simplex virus (hsv) 1 or 2. the amount of interferon produced by the hsv-exposed monocytes was found to be donor-specific and was similar whether tested with hsv 1 or hsv 2. the interferon levels in repeated blood specimens from a given donor were relatively stable within an observation period of a few months. the survey was expanded by testing 27 persons with history of recurrent herpes disease ... | 1988 | 2842448 |
| phosphonoformate and phosphonoacetate derivatives of 5-substituted 2'-deoxyuridines: synthesis and antiviral activity. | the synthesis of potential "combined prodrugs" wherein phosphonoformate or phosphonoacetate was attached to the 5'-position of 2'-deoxyuridine, 2'-deoxythymidine, 5-iodo-2'-deoxyuridine (idu), 5-(2-chloroethyl)-2'-deoxyuridine (cedu), or 5-(2-bromovinyl)-2'-deoxyuridine (bvdu) or to the 3'-position of cedu is described. the antiviral activities of these derivatives and of reference compounds were compared in vero, hep-2, and primary rabbit kidney cells against herpes simplex virus types 1 and 2 ... | 1988 | 2842506 |
| evaluation of macrophage antiviral activity in patients affected by neoplasia. | the intrinsic antiviral activity of macrophages has been studied in healthy donors and in patients affected by breast cancer and melanoma. in vitro differentiated macrophages from blood-derived monocytes were infected with measles virus, herpes simplex virus type 2 and adenovirus 17. the challenge was carried out with different multiplicities of infection and the synthesis of virus was tested by evaluating the single cycle growth curve in 24 h. the results obtained show that the restriction of v ... | 1988 | 2842553 |
| increased efficacy of phosphonoformate and phosphonoacetate inhibition of herpes simplex virus type 2 replication by encapsulation in liposomes. | phosphonoformate and phosphonoacetate encapsulated in liposomes have substantially greater activity against herpes simplex virus type 2 in vero cell tissue culture than the nonencapsulated compounds at the same dose. encapsulation of phosphonoformate in liposomes resulted in a 30-fold increase of the antiviral effect with no increase in cytotoxicity measured by inhibition of thymidine incorporation into normal vero cells. thus, the selectivity of the liposomal drug increased 27-fold compared wit ... | 1988 | 2843083 |
| inducible expression of herpes simplex virus type 2 glycoprotein gene gg-2 in a mammalian cell line. | the gg-2 glycoprotein gene of herpes simplex virus type 2 (hsv-2) was cloned into the mammalian expression vector pmsg under the control of the inducible mouse mammary tumor virus promoter. transfection of this cloned gg-2 construct into nih 3t3 cells resulted in the stable expression of gg-2 upon induction with dexamethasone. in addition, the 104,000-molecular-weight (104k) and 72k gg-2 precursors as well as the 34k secreted component were generated in the transformed cells. the synthesis of gg ... | 1988 | 2843667 |
| effectiveness of a procedure for disinfecting the vaginal photoplethysmograph contaminated with herpes simplex virus type 2. | to test the effectiveness of a disinfecting procedure involving 2% glutaraldehyde, a vaginal photoplethysmograph was contaminated with a known amount of herpes simplex type 2 (hsv-2). the vaginal photoplethysmograph was then put through the disinfection procedure. two virus solutions were tested, one designed to approximate the concentration found in a naturally occurring infection (low inoculum), the other with 100 times more virus (high inoculum). varying lengths of exposure to glutaraldehyde ... | 1988 | 2844137 |
| multicentric intraepithelial neoplasia involving the vulva. clinical features and association with human papillomavirus and herpes simplex virus. | sixteen of 46 patients (35%) with grade 3 vulvar intraepithelial neoplasia (vin 3) were found to have an additional site of lower genital tract squamous cell neoplasia, primarily in the cervix. the frequency of multicentricity decreased significantly with age. in addition, patients with multicentric disease (involving the vagina and/or cervix in addition to the vulva) had a significantly higher frequency of multifocal disease involving the vulva (involving more than one location on the vulva) an ... | 1988 | 2844383 |
| interaction of herpes simplex virus type 2 with a rat glioma cell line. | the interaction between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and two neural cell lines, mouse neuroblastoma (n1e-115) and rat glioma (c6-bu-1), was investigated. n1e-115 cells were permissive to both types of hsv. in c6-bu-1 cells, on the other hand, all the hsv-1 strains tested so far showed persistent infection, and the infectious virus of hsv-2 strains disappeared spontaneously. the hsv-2-infected c6-bu-1 cells were positive for hsv-2-specific dna sequences, virus-specific r ... | 1988 | 2850449 |
| infection of human thymic epithelial cells by human cytomegalovirus and other viruses: effect on secretion of interleukin 1-like activity. | cultures of human thymic epithelial (te) cells are able to produce a interleukin 1 (il-1) like activity. this il-1 activity can be detected either using mouse thymocytes in a traditional il-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. production of il-1 activity by te cells was found to be maximal between 3 and 4 weeks after culture initiation. human thymocytes worked best as targets in an il-1 assay, when these cells were derived from donors you ... | 1988 | 2844452 |
| herpes simplex virus ii antigen in adenocarcinoma of the uterine cervix. | 1988 | 2844663 | |
| mutational analysis of the herpes simplex virus type 1 trans-inducing factor vmw65. | the herpes simplex virus type 1 (hsv-1) polypeptide vmw65 is a structural component of the virus particle and is also responsible for trans-induction of immediate early (ie) transcription. functional domains of this polypeptide were investigated by constructing a series of 10 plasmids each with a 12 bp insertion in the gene encoding vmw65. plasmids were analysed for their ability to stimulate ie transcription in short term transfection assays, and the altered vmw65 polypeptides were assayed for ... | 1988 | 2844968 |
| detection of herpes simplex virus type 1 in herpetic ocular diseases by dna-dna hybridization using a biotinylated dna probe. | a diagnostic hybridization assay for detecting herpes simplex virus type 1 (hsv-1) in ocular specimens was developed using cloned viral dna as a probe. this hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target dna by a streptavidin/alkaline phosphatase system. the time required for performing this assay system is only two days. this assay system could detect a probe which had been hybridized to as little as 1 pg of homologous dna and did not cross-rea ... | 1988 | 2844977 |
| c3b receptor activity on transfected cells expressing glycoprotein c of herpes simplex virus types 1 and 2. | glycoprotein c from herpes simplex virus type 1 (gc-1 from hsv-1) acts as a receptor for the c3b fragment of the third component of complement on hsv-1-infected cell surfaces. direct binding assays with purified gc-1 and c3b demonstrate that other viral and cellular proteins are not required for this interaction. although c3b receptor activity is not expressed on hsv-2-infected cell surfaces, purified gc-2 specifically binds c3b in direct binding assays, suggesting that gc-1 and gc-2 are functio ... | 1988 | 2845122 |
| anti hsv-2 properties of amniotic fluid. | some antiviral properties of amniotic fluid (af) were studied. a close relationship between maternal blood and af antiviral antibodies was seen. substances other than antibodies which could have an effect on viruses were assumed to be present in af. using hsv2 as a model, we found a virus-neutralizing activity in 6 out of 7 specimens studied which does not appear to be related to antibodies. | 1988 | 2845231 |