Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| genital infections due to herpes simplex virus type 2 in male guinea pigs. | a small-animal model of genital herpes simplex virus (hsv) infection has not been available for investigating the pathogenesis of disease in males. male hartley guinea pigs were inoculated on the medial thigh with 10(5)-10(6) plaque-forming units of hsv type 2. animals developed vesicular lesions in the genital area, including the penis. transient urinary retention, hindlimb paralysis, and acute urethral shedding of hsv also occurred. during acute infection, virus was detected in neural and geni ... | 1989 | 2535869 |
| viral agents in two-stage cervical carcinogenesis: an experimental study in mice. | the role of chemical and viral agents in the development of cervical cancer in mice was studied by repeated carcinogen applications, repeated intravaginal instillations of hsv type ii virus, and single carcinogen application as initiating agent followed by repeated instillations of virus as promoter. in all the animals studied, repeated applications of 9,10-dimethylbenzanthracene (dmba) induced dysplastic conditions of the cervix and vagina, mild, moderate, and severe, as well as a large number ... | 1989 | 2535998 |
| immunogenicity of herpes simplex virus glycoprotein gb-1-related protein produced in yeast. | a protein related to glycoprotein b of herpes simplex virus type 1 (hsv-1) produced in yeast (ygb-1) was purified with an immunoadsorbent. the molecular weight of the purified ygb-1 as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis was 96,000. mice injected twice with ygb-1 adsorbed to alum developed elisa antibody to ygb-1, neutralizing antibody to hsv-1 and a lymphoproliferative response to ygb-1 and hsv-1. the immunized mice were protected against intraperitoneal and ... | 1989 | 2546329 |
| identification, transfer, and characterization of cloned herpes simplex virus invasiveness regions. | following peripheral inoculation of experimental animals, herpes simplex virus type 2 (hsv-2) strains are more virulent than hsv-1 strains, and clinical studies suggest that they possess enhanced virulence in humans. one dramatic type-specific difference in virulence is observed following inoculation of the chorioallantoic membrane (cam) of the chicken embryo: hsv-2, but not hsv-1, makes large pocks on the cam, invades the mesoderm, generalizes in the embryo, and kills the chicken. these propert ... | 1989 | 2536826 |
| the antiviral activity of 9-beta-d-arabinofuranosyladenine is enhanced by the 2',3'-dideoxyriboside, the 2',3'-didehydro-2',3'-dideoxyriboside and the 3'-azido-2',3'-dideoxyriboside of 2,6-diaminopurine. | the 2',3'-dideoxyriboside (dddapr), 2',3'-didehydro-2',3'-dideoxyriboside (ddedapr) and 3'-azido-2',3'-dideoxyriboside (azdddapr) of 2,6-diaminopurine have been previously recognized as potent inhibitors of human immunodeficiency virus replication. these compounds are also potent inhibitors of adenosine deaminase and inhibit the deamination of 9-beta-d-arabinofuranosyladenine (araa). dddapr, ddedapr and azdddapr markedly potentiate the antiviral activity of araa against herpes simplex virus type ... | 1989 | 2538128 |
| short, duplicated sequence indicative of the recombinogenicity of the junction between a unique and an inverted repeat sequence in the s component of the herpes simplex virus type 1 genome. | a herpes simplex virus type 1 (hsv-1) strain, b3, was found to have a short duplication on the left junction between the unique sequence (us) and the inverted repeat sequence (rs) in the s component of the genome dna. a short region of rs contiguous to the left us-rs junction was duplicated in b3. based on the nucleotide sequences in and around the us-rs junctions of b3 and other hsv-1 strains, a concept of junction stretch was proposed. the organization of junction stretch is rs side 5'-(g or a ... | 1989 | 2539490 |
| serological evidence for variation in the incidence of herpesvirus infections in different species of apes. | sera from captive lowland gorillas, chimpanzees, orangutans, and gibbons were screened by enzyme-linked immunosorbent assay (elisa) for antibody to herpesviruses serologically related to human herpes simplex virus types 1 and 2 (hsv-1, hsv-2), a baboon virus (sa8), and a macaque herpesvirus (b virus). the incidence of herpesvirus antibodies varied considerably among the different species, gorillas having the highest incidence of seropositivity (65.4%) and orangutans the lowest. the virus specifi ... | 1989 | 2546978 |
| herpes simplex virus type 2 mutants with deletions in the intergenic region between icp4 and icp22/47: identification of nonessential cis-acting elements in the context of the viral genome. | in herpes simplex virus type 2, the mrnas of icp4 and icp22/47 are divergently transcribed and their transcription initiation sites are separated by 750 base pairs (l. j. whitton and j. b. clements, nucleic acids res. 12:2061-2078, 1984). this 750-base-pair region contains many recognized cis-acting elements, including two tata boxes, numerous sp1-binding sites, four taatgarat motifs, at least one icp4-binding site, and two origins of replication (oris) linked in tandem. in this report, we descr ... | 1989 | 2539500 |
| analysis of unselected hsv-1 mckrae/hsv-2 hg 52 recombinants demonstrates preferential recombination between intact genomes and restriction endonuclease fragments containing an origin of replication. | to identify viral genes involved in reactivation of herpes simplex virus from latency, intertypic hsv-1 strain mckrae/hsv-2 strain hg 52 recombinants were selected following cotransfection of intact mckrae dna and xbai or hpai cleaved hg 52 dna. eleven separately obtained recombinants containing hg 52 inserts between 0.35-0.56 and/or 0.82-1.0 map units (mu) were isolated. it was noted that with hpai digested hg 52 dna, only recombinants containing type 2 inserts from hpai d (0.35-0.57) and/or co ... | 1989 | 2541672 |
| regulation of expression of herpes simplex virus (hsv) glycoprotein d in vaccinia recombinants affects their ability to protect from cutaneous hsv-2 disease. | the effect of regulation of herpes simplex virus (hsv) type 1 glycoprotein d (gd-1) gene expression on hsv-specific immune response and protection from cutaneous hsv-2 disease was studied using vaccinia virus recombinants containing gd-1 under the control of early (vp176) or late (vp254) vaccinia virus promoters. expression of gd-1 in vp176-infected cells was first observed at 2 h after infection. it did not depend on viral dna replication. in vp254-infected cells, gd-1 was first observed at 24 ... | 1989 | 2538519 |
| replacement of the pseudorabies virus glycoprotein giii gene with its postulated homolog, the glycoprotein gc gene of herpes simplex virus type 1. | giii, the major envelope glycoprotein of pseudorabies virus (prv), shares approximately 20% amino acid similarity with glycoprotein gc of herpes simplex virus type 1 (hsv-1) and hsv-2. we describe here our first experiments on the potential conservation of function between these two genes and gene products. we constructed prv recombinants in which the giii gene and regulatory sequences have been replaced with the entire hsv-1 gc gene and its regulatory sequences. the gc promoter functions in the ... | 1989 | 2548015 |
| promoting effect of croton oil on the induction of cervical and vaginal cancers with herpes simplex virus types 1 and 2 in mice. | hybrid swiss virgin mice (kuan-min strain) were challenged intravaginally with uv-inactivated herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) twice a week for 16 times and subsequently with croton oil or control medium twice a week for 27 times. after a period of 180 days the exposure to hsv-2 plus control medium induced premalignant and malignant lesions of the cervix and vagina in 50.0% of the mice. the exposure to hsv-2 plus croton oil induced similar lesions in 78.2% of the mice. the e ... | 1989 | 2539313 |
| a herpes simplex virus type 2 variant in which a deletion across the l-s junction is replaced by single or multiple reiterations of extraneous dna. | we have isolated and characterized a novel variant of herpes simplex virus type 2 (hsv-2) strain hg52 which has a deletion of 13.5 kb across the l-s junction of the genome, resulting in all of the irl region, half of the irs region and the intervening l-s junction 'a' sequence(s) being removed. the deleted dna has been replaced by single or multiple (up to 14) reiterations of a dna sequence approximately 1 kb in length. individual genomes within the population range in size from approximately 12 ... | 1989 | 2549183 |
| two colour dna in situ hybridization for the detection of two viral genomes using non-radioactive probes. | methods for the simultaneous detection of two virus types in cytological preparations or tissue sections by non-radioactive in situ hybridization were investigated. as a model system, caski cells, which have human papilloma virus type 16 (hpv16) dna integrated in their cellular genome, were in vitro infected with herpes simplex virus 2 (hsv2). dna probes for both viruses were labeled with biotin, acetylaminofluorene (aaf), and transaminated-sulfonated cytosine (ts-modified). best results were ob ... | 1989 | 2542189 |
| serum iga antibodies in hsv asymptomatic genital infections. | cervico-vaginal swabs and serum samples from 81 women without herpes-associated clinical symptoms were examined for virus isolation and for the presence of iga antibodies to herpes simplex virus (hsv) using the indirect fluorescent antibody technique. nineteen of the 81 women were followed up for 2 years. blood samples and swabs from cutaneous lesions were obtained from another group of 20 women (medical staff) with herpes labialis. by analysing the specific anti-hsv iga antibodies, a higher geo ... | 1989 | 2542431 |
| antigen-presenting liposomes are effective in treatment of recurrent herpes simplex virus genitalis in guinea pigs. | the therapeutic and immunologic effects of a liposome preparation containing both a macrophage activator, muramyl-tripeptide-phosphatidylethanolamine, and a recombinant antigen, glycoprotein d of herpes simplex virus type 1, have been investigated. this preparation was tested in vitro for the ability to stimulate peripheral blood lymphocytes and in vivo for the control of recurrent herpes genitalis in guinea pigs. our results show that the liposome-antigen-adjuvant preparation is capable of enha ... | 1989 | 2542605 |
| transmission of genital herpes by donor insemination. | this report describes a donor in a therapeutic donor insemination program who asymptomatically acquired a primary herpes simplex virus type 2 (hsv-2) infection from his long-standing sexual partner. his fresh semen was used to inseminate two hsv-seronegative recipients; in one a primary hsv-2 infection developed, and in one it did not. direct evidence of transmission from donor to recipient was documented by restriction enzyme analysis of the hsv-2 isolates obtained from the donor's semen and fr ... | 1989 | 2542632 |
| herpes simplex virus glycoprotein immunotherapy of recurrent genital herpes: factors influencing efficacy. | recombinant herpes simplex virus type 1 (hsv-1) glycoproteins b (gb) and d (gd) were used as immunotherapeutic agents for the treatment of recurrent genital herpes in guinea pigs. administration of a gbgd vaccine eight to 21 days after intravaginal hsv-2 inoculation significantly increased the titer of anti-hsv antibodies (p less than 0.005) while significantly reducing the frequency of subsequent herpetic recurrences (p less than 0.05). the effectiveness of gbgd immunotherapy was influenced by ... | 1989 | 2545166 |
| antigen-presenting capacity of epidermal cells infected with vaccinia virus recombinants containing the herpes simplex virus glycoprotein d, and protective immunity. | we studied the association of herpes simplex type 1 (hsv-1) glycoprotein d (gd-1) expression in epidermal cells (ec) with virus-specific immunity and protection of mice from fatal hsv-2 challenge. vaccinia virus recombinants containing gd-1 under the control of an early (vp176) or late (vp254) vaccinia virus promoter were used. mature gd-1 protein was expressed in vp176-infected ec and they had accessory cell function for hsv-2-induced t cell proliferation of immune lymph node cells (lnc). it wa ... | 1989 | 2550579 |
| rapid detection of herpes simplex virus with fluorescein-labeled helix pomatia lectin. | the use of fluorescein-conjugated helix pomatia lectin was shown to be as effective as fluorescein-conjugated monoclonal antibody reagents for the detection and differentiation of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in mrc-5 cell culture. cells infected with hsv-1 generally displayed a pattern of nongranular or diffuse fluorescence, while cells infected with hsv-2 were identified by the production of fluorescent grains and flecks. this unique nonimmunological reagent, when u ... | 1989 | 2545739 |
| protein kinase activity associated with the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10). | the large subunit of the herpes simplex virus type 2 (hsv-2) ribonucleotide reductase (rr1) is demonstrated to possess serine/threonine-specific kinase activity. computer-assisted sequence analysis identified regions within the amino terminus of icp10 that are homologous to the catalytic domain of known protein kinases (pks). an in vitro kinase assay confirmed the ability of icp10, immunoprecipitated from either hsv-2-infected cells or from cells transfected with an icp10 expression vector, to a ... | 1989 | 2545912 |
| herpes simplex virus type 2 primes mouse macrophages for an early and genetically determined respiratory burst mediated by interferon-alpha/beta. | the influence of infection by herpes simplex virus type 2 (hsv-2) on the respiratory burst capacity of mouse macrophages was studied by luminol-dependent chemiluminescence with phorbol myristate acetate (pma) as trigger. peritoneal cells from virus-infected mice were strongly primed for a respiratory burst during the acute phase of the infection. by 12 h after infection the response had increased 40-fold over control values. most of the response was elicited by mononuclear phagocytes. when resti ... | 1989 | 2543784 |
| a seroepidemiologic survey of the prevalence of herpes simplex virus type 2 infection in the united states. | the prevalence of infection with the genital herpes simplex virus type 2 (hsv-2) has been difficult to ascertain, primarily because of the large percentage of subclinical cases and the limitations in specificity of serologic assays for antibody to hsv-2. to obtain an improved estimate of the distribution of hsv-2 infection in the united states, we used an hsv type-specific antibody assay to test serum samples from 4201 participants in the second national health and nutrition examination survey. ... | 1989 | 2543928 |
| [correlation of human papilloma virus (hpv) and herpes simplex virus-2 (hsv-2)in the viral etiology of carcinoma of uterine cervix]. | hpv dna and hsv-2 antigens were detected in a series of cervical lesions by dna hybridization and pap stain techniques in order to investigate the possible role of hpv and hsv-2 in the viral etiology of cervical carcinoma. the correlation of hpv dna sequence and hsv-2 antigens was analyzed. the results showed that the positive rates of hpv 16, 18 and 11 in cervicitis, dysplasia and cervical cancer were 13%; 0, 13%; 17%, 17%, 17%; 43%, 8%, 4%; and hsv-2 antigens were found in 41% of cervicitis, 5 ... | 1989 | 2553361 |
| viral antigen stimulation of the production of human monokines capable of regulating hiv1 expression. | we have previously described model systems for cytokine-induced regulation of chronically hiv-infected promonocyte and t cell clones. using these systems, we have shown that monokines contained in supernatants from lps-stimulated human monocyte/macrophages (mo) up-regulate hiv expression, reflected by an increase in reverse transcriptase activity, viral rna levels, and expressed viral proteins. current studies were designed to determine whether viral ag can interact with mo and secondarily affec ... | 1989 | 2544645 |
| vero cells co-infected with chlamydia trachomatis and herpes simplex virus type 2: a scanning and transmission electron microscope study. | vero (african green monkey kidney) cells infected with chlamydia trachomatis (serovar l2) (ct-l2) for 48 hr and superinfected with herpes simplex virus type 2 (hsv-2) were fixed 24 hr later and examined with the transmission electron microscope (tem) and scanning electron microscope (sem). ultrastructural examination of the co-infected cells showed that, although many ct-l2 inclusions were present, most were empty of reticulate bodies or elementary bodies. however, large numbers of viruses were ... | 1989 | 2545004 |
| sexual activity, contraception, genital infections, and cervical cancer: support for a sexually transmitted disease hypothesis. | a case-control study was conducted in utah between 1984 and 1987 to examine risk factors for cervical cancer. interviews were completed with 266 histologically confirmed carcinoma in situ and invasive squamous cell cervical cancer cases who were categorically matched by age to 408 controls. among the factors identified as altering risk for cervical cancer, after adjustment for age, education, church attendance, and cigarette smoking, were: having numerous sex partners (odds ratio (or) = 8.99 for ... | 1989 | 2546422 |
| identification of immediate-early-type cis-response elements in the promoter for the ribonucleotide reductase large subunit from herpes simplex virus type 2. | regulation of the expression of the herpes simplex virus (hsv) type 2 large subunit of ribonucleotide reductase (icp10) gene was studied directly by immunofluorescence or by chloramphenicol acetyltransferase analysis with hybrid icp10 promoter constructions. in vero cells, cotransfection with dna encoding hsv ie110 or vmw65 proteins or hcmv ie2 enhanced expression at least 10-fold. in contrast, expression was minimally enhanced by dna encoding ie175 at low doses and slightly reduced at high dose ... | 1989 | 2542589 |
| mild acidic ph inhibition of the major pathway of herpes simplex virus entry into hep-2 cells. | penetration of the kos strain of herpes simplex virus type 1 (hsv-1) and the ms and 333 strains of herpes simplex virus type 2 (hsv-2) into hep-2 cells at ph 6.3 was at least 100-fold less efficient than at ph 7.4. penetration of two low passage clinical isolates was completely blocked at ph 6.3. the syncytium-forming hsv-1 strains gc and mp were less sensitive than kos to the mild acidic conditions. the inhibition was completely reversed upon neutralization of the medium. penetration was assaye ... | 1989 | 2543762 |
| the herpes simplex virus type 2 (hg52) variant jh2604 has a 1488 bp deletion which eliminates neurovirulence in mice. | the herpes simplex virus type 2 (hg52) deletion variant jh2604 is avirulent (ld50 greater than 10(7) p.f.u./mouse) for mice compared to the parental wild-type virus (ld50 less than 10(2) p.f.u./mouse) and fails to replicate in vivo. jh2604 has a 1488 bp deletion within the 3 kb bamhi v fragment (0 to 0.02 and 0.81 to 0.83 map units) which removes one copy of the 17 bp direct repeat dr1 element of the 'a' sequence and terminates 522 bp upstream of the 5' end of the immediate early gene 1. in vivo ... | 1989 | 2555437 |
| evaluation of a hsv specific monoclonal antibody reagent for laboratory diagnosis of herpes simplex virus infection. | a new fitc-conjugated hsv specific monoclonal antibody reagent (syva co., palo alto, ca) was evaluated for the confirmation of hsv clinical isolates. the reagent was also compared to type-specific monoclonal antibodies for the pre-cpe detection of hsv from clinical specimens in centrifugation culture and by direct examination of specimens smears by direct immunofluorescent antibody staining (dfa). hsv was isolated from 75 of 232 specimens (32%). all 75 isolates were confirmed with both the type- ... | 1989 | 2556235 |
| herpes simplex virus type 1 immediate-early protein vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system. | reactivation of latent herpes simplex virus type 2 (hsv-2) by the immediate-early protein vmw110 was studied by using an in vitro latency system. adenovirus recombinants that express vmw110 reactivated latent hsv-2. an hsv-1 mutant possessing a deletion in a carboxy-terminal region of vmw110 reactivated latent hsv-2, whereas mutant fxe, which has a deletion in the second exon, did not. therefore, vmw110 alone is required to reactivate latent hsv-2 in vitro, and the region of vmw110 defined by th ... | 1989 | 2545921 |
| in vitro evaluations of condoms with and without nonoxynol 9 as physical and chemical barriers against chlamydia trachomatis, herpes simplex virus type 2, and human immunodeficiency virus. | simulated in vitro intercourse conditions demonstrated that unlubricated latex condoms provide an effective physical barrier to high concentrations of chlamydia trachomatis, herpes simplex virus type 2, and human immunodeficiency virus. however, since condoms can be damaged after manufacturing inspection and prior to use, latex condoms alone should not be perceived as absolute protection against stds. nonoxynol 9 used in conjunction with condoms provided additional, yet still not foolproof, prot ... | 1989 | 2545003 |
| inhibition of herpes simplex virus infection by pine cone antitumor substances. | several antitumor substances extracted from cones of various pine trees inhibited the plaque formation of herpes simplex virus types 1 and 2 (hsv-1, hsv-2) strains in african green monkey kidney cells and human adenocarcinoma cells. the 50% effective dose of the most active fraction, fr. vi (0.3 micrograms/ml) was 0.001 times its 50% cytotoxic dose (greater than 300 micrograms/ml). the anti-hsv activity of various pine cone extracts increased with their acidity. identification of the polyphenol ... | 1989 | 2546481 |
| active replication of human immunodeficiency virus type 1 by peripheral blood mononuclear cells following coincubation with herpes viruses. | patients with acquired immunodeficiency syndrome (aids) commonly suffer from opportunistic infections associated with members of the herpes virus family. to investigate whether certain of these other viruses might have an effect on the ability of the human immunodeficiency virus type 1 (hiv-1) to replicate, we coincubated peripheral blood mononuclear cells (pbmc) from nine hiv-1-seropositive donors with live preparations of various herpes viruses. in seven of nine cases, exposure of pbmc to prep ... | 1989 | 2557380 |
| biological and biochemical characterization of clinical isolates of herpes simplex virus type 2 resistant to acyclovir. | a series of clinical isolates of herpes simplex virus type 2 were taken from a patient with chronic lymphocytic leukemia. acyclovir (acv) susceptibility assays revealed that some isolates were resistant to acv and cross-resistant to ganciclovir but not to phosphonoacetic acid. the nature of the resistance was examined further. a number of cloned variants were generated, and thymidine kinase and dna polymerase assays were carried out. variants that were resistant to acv were found to be thymidine ... | 1989 | 2546486 |
| evidence of the non-infectivity of herpes simplex viral particles in trichomonas vaginalis. | fan et al., employing an indirect immunofluorescent antibody (ifa) technique, reported the presence of herpes simplex virus type 2 (hsv-2) in trichomonas vaginalis. however, using the same method, we found that the protozoa showed autofluorescence immediately after acetone fixation. in order to demonstrate the non-infectivity of hsv-2 in t. vaginalis, several other methods were performed in this study. trichomonads were experimentally incubated together with hsv-2 and examined after immunofluore ... | 1989 | 2558006 |
| [neutralizing activity against herpes simplex in maternal milk]. | human milk could play a role in the protection of the newborn from herpes simplex virus 2 contamination. neutralizing activity against herpes simplex virus 2 was measured in 94 human milk samples and in 31 sera from healthy women. a weak neutralizing activity was detected in 48 milk samples. they were neither correlated to anti-herpes simplex virus antibodies found in milk, nor to the neutralizing activity in sera. the origin and non immunologic nature of this activity are discussed. | 1989 | 2546513 |
| synthesis and antiviral activity of the enantiomeric forms of carba-5-iodo-2'-deoxyuridine and carba-(e)-5-(2-bromovinyl)-2'-deoxyuridine. | both enantiomers of the carbocyclic analogues of 5-iodo-2'-deoxyuridine (14 and ent-14) and of (e)-5-(2-bromo-vinyl)-2'-deoxyuridine (16 and ent-16) were synthesized by using (+)- or (-)-endo-norborn-5-en-2-yl acetate or butyrate, respectively, as starting materials. against herpes simplex virus type 1 (+)-c-bvdu (16) was only slightly less active than bvdu itself, whereas (-)-c-bvdu (ent-16) proved to be 10-400-fold less effective, depending on the strain investigated. against hsv-2 both (+)- a ... | 1989 | 2547072 |
| human topoisomerase 1 messenger rna is not destabilized by the herpes simplex virus type 2 virion-associated shut-off function. | a cdna for human topoisomerase i (topo 1) was used to identify a 4.1 kb polyadenylated topo 1 mrna in methotrexate-resistant human kb cells that are permissive for herpes simplex virus type 2 (hsv-2) infection. using these cells, no effect of the hsv-2-associated early shut-off function on levels of topo-1 mrna was observed up to 6 hours postinfection, whereas the actin mrna level was 22% cellular transcripts are susceptible. the level of several host-cell polyadenylated rnas detected as cdna cl ... | 1989 | 2554584 |
| smoking and infectious agents and risk of in situ cervical cancer in sydney, australia. | in a study of 116 in situ cervical cancer patients and 193 matched community controls in sydney, australia, smoking was found to be a major risk factor. current smokers had a adjusted relative risk [rr] of 4.5 compared to nonsmokers [95% confidence interval (ci) 2.2-9.1] and exsmokers a rr of 1.3 [95% ci 0.6-3.0]. there was a stepwise dose-response relationship between risk and number of cigarettes smoked (30+ cigarettes/day, rr = 5.1, 95% ci 1.5-17.3); this dose-response relationship was more m ... | 1989 | 2547521 |
| efficacy of oxetanocin g against herpes simplex virus type 2 and murine cytomegalovirus infections in mice. | 1989 | 2547744 | |
| enhancement of herpes simplex virus type 2 (hsv-2) dna synthesis in infected cells that constitutively express the bglii-n region of the hsv-2 genome. | the bglii-n fragment of the herpes simplex virus type-2 (hsv-2) genome encodes one of two known transforming regions of this dna virus. in this study, we report the derivation of hela s3 cells (2dc4) that stably express the hsv-2 bglii-n region, including the small subunit of hsv-2 ribonucleotide reductase (rr). superinfection of the 2dc4 cells with wild-type hsv-2 resulted in the efficient induction of hsv-2-encoded icp10, dna polymerase, and thymidine kinase. the amount of hsv-2 dna synthesis ... | 1989 | 2548338 |
| retention and expression of the left end subfragment of the herpes simplex virus type 2 bglii n dna fragment do not correlate with tumorigenic conversion of nih 3t3 cells. | cotransfection experiments have been carried out using recombinant plasmids pag60, conferring resistance to antibiotic g418, and pxho3 which contains the left end subfragment (map coordinates 0.583 to 0.596) of the transforming herpes simplex virus type 2 bglii n dna fragment and encodes the 36k polypeptide associated with the viral ribonucleotide reductase activity. several nih 3t3 cell clones resistant to g418 and having morphological changes commonly observed for transformed nih 3t3 cells wer ... | 1989 | 2549186 |
| [detection of igg and igm antibodies to herpes simplex virus type 1 and type 2 in human serum using an immunoenzyme method (elisa) and the complement fixation reaction]. | on the basis of borderline optical density values (od) in an enzyme-linked immunosorbent assay (elisa) resulting from comparative testing of this method with complement fixation reactions (cf), determination was made of a set criteria for negative, weakly positive and positive results of igg and igm antibody detection of herpes simplex type 1 (hsv 1), respectively type 2 (hsv 2). of the 192 examined specimens by elisa, 69.2% and 49.5% igg hsv 1 and hsv 2 antibodies were found respectively. the n ... | 1989 | 2549311 |
| [epidemiological evaluations of human immunodeficiency virus, herpes simplex virus type 1 and 2 and cytomegalovirus infections in drug addicts]. | eighty-eight drug addicts from the "ban center" in torre annunziata (naples) and 88 normal subjects pair-matched for age and sex were tested for igg to human immunodeficiency virus (hiv), herpes simplex virus (hsv) type 1 and 2 and cytomegalovirus (cmv). a high prevalence of subjects with antibodies to hsv-1 and cmv (80.7% and 65.9%) were recorded in the control group testifying to the high level of these infections in campania. prevalences were higher in drug addicts, and drug abuse was identif ... | 1989 | 2562004 |
| protection and serum antibody responses in guinea-pigs and mice immunized with hsv-1 antigen preparations obtained using different detergents. | guinea-pigs immunized with a zwitterionic detergent-extracted hsv-1 antigen preparation responded with eia and nt serum antibody titres that were significantly greater than those elicited by a non-ionic detergent-extracted antigen preparation inoculated using a similar dosage schedule. following intravaginal challenge of the guinea-pigs with hsv-2 (strain sh/b), there was no statistically significant difference in the protection afforded to these animals by the two antigen preparations, although ... | 1989 | 2554607 |
| analysis of dna synthesis in herpes simplex virus infected cells by dual parameter flow cytometry. | the dna incorporation of a thymidine analogue bromodeoxyuridine (brdurd) in herpes simplex virus type 2 (hsv-2) infected cervical cancer cell lines (caski and c-33 a) was studied by dual parameter flow cytometry. hsv-2 infection resulted in an exponential increase in dna synthesis in the caski cells. in the less permissive c-33 a cells the proportion of dna-synthesizing cells cycled during hsv-2 infection. the inhibition of viral dna synthesis by phosphonoformate (pfa) was able to inhibit the vi ... | 1989 | 2554855 |
| herpes simplex virus type 2 antibodies: high prevalence in monogamous women in costa rica. | we studied the prevalence of antibody to herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) in 766 randomly selected costa rican women 25-59 years of age in a national household survey in 1984-1985. overall, 97.1% were seropositive for hsv-1 and 39.4% for hsv-2. only 1.1% of hsv-2 seropositive women gave a history of symptomatic genital herpes. hsv-2 virus antibody increased with age and with the number of lifetime sexual partners. hsv-2 seroprevalence among women who reported only 1 lifetime ... | 1989 | 2549796 |
| recovery frequencies of herpes simplex virus types 1 and 2 from symptomatic and asymptomatic genital herpes cases and antiviral sensitivities of isolates. | herpes simplex viruses were isolated from 40.8 to 56.0 per cent of the patients with genital herpes. the frequency of recovery seemed to be higher in females than in males, particularly during the first episode of infection. asymptomatic shedding of the virus from female genitalias was approximately 0.7 per cent. herpes simplex virus type 2 represented 98.4 per cent of all isolates and the remaining isolates were type 1. these isolates exhibited a wide range of sensitiveness to 9-(2-hydroxyethox ... | 1989 | 2555430 |
| some immunological characteristics of subjects suffering from frequent herpes simplex virus recrudescences. | some parameters of specific and non-specific immunity were tested in a group of 44 subjects suffering from frequent herpes simplex type 1 (hsv-1) or herpes simplex type 2 (hsv-2) recrudescences. the tests performed included determinations of (i) hsv complement-independent and complement-dependent neutralizing antibodies, (ii) antibodies to glycoprotein c of hsv-1 and glycoprotein g of hsv-2, (iii) antibodies to viral capsid and early antigens of epstein-barr virus, (iv) antibodies to tetanus tox ... | 1989 | 2576584 |
| viral etiology of cervical carcinoma. human papilloma virus and herpes simplex virus type 2. | the possible role of human papilloma virus (hpv) and herpes simplex virus type 2 (hsv-2) in the viral etiology of cervical carcinoma was investigated a series of cervical lesions were studied for the presence of hpv and hsv-2 dna sequences as well as hpv and hsv-2 antigens by dna dot blot hybridization technique and high-specificity pap staining method. the results obtained were correlated with the histologic diagnosis. hpv 16 dna sequences detected in cervical carcinoma biopsies were 43%, where ... | 1989 | 2550184 |
| clinical and serological outcome of genital herpes simplex virus (hsv) type 2 inoculation following oral hsv type 1 infection in guinea-pigs. | the clinical and serological outcome of genital herpes simplex virus type 2 (hsv-2) inoculation in animals previously orally infected with hsv type 1 was evaluated. a prior hsv-1 oral infection modified the genital hsv-2 infection so that only four of 18 (22%) animals were initially symptomatic although all but one animal shed hsv-2 from the cervicovaginal area for at least 5 days following inoculation. three of four animals with symptomatic initial disease also developed recurrences, as did an ... | 1989 | 2550572 |
| evaluation of a new herpes simplex virus typing reagent for tissue culture confirmation. | monoclonal antibody typing reagents for herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) from california integrated diagnostics, inc. (berkeley, ca) were compared to two other commercially available hsv monoclonal antibody typing reagents. of the 105 specimens tested, of which 81 were positive for hsv, there was 100% agreement with all three typing reagents. | 1989 | 2551569 |
| isolation of a herpes simplex virus type 2 that is retinovirulent in mice. | the virulence of a herpes simplex virus type 2 (hsv-2) isolated from the urine of a patient (sl) with acquired immunodeficiency syndrome (aids) and bilateral acute retinal necrosis (arn), was investigated in mice. the ratio of plaque forming units (pfu) in fibroblasts to the 50% lethal dose (ld50) of hsv-2(sl) in mice was 10 fold more than the pfu to ld50 ratio of a neurovirulent hsv-2, strain 186. further, hsv-2(sl) caused retinitis with and without lethal encephalitis in mice inoculated intrac ... | 1989 | 2551573 |
| inhibition of herpes simplex virus infection by tannins and related compounds. | several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (hsv-1, hsv-2)-infected african green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-hsv activity. their 50% effective doses (0.03-0.1 microgram/ml) were by two-three orders of ... | 1989 | 2552915 |
| herpes simplex virus stromal keratitis is not titer-dependent and does not correlate with neurovirulence. | we developed a murine model of ocular herpes simplex virus (hsv) disease which is particularly suited for testing stromal keratitis because most animals show some evidence of infection. using this model, we characterized the ocular disease patterns caused by ten recent low-passage clinical isolates of hsv-1, as well as those caused by the established laboratory strains hsv-1 kos and hsv-2 333. viral strains were evaluated for their ability to cause stromal keratitis, blepharitis, vascularization ... | 1989 | 2556353 |
| detection of herpes simplex virus dna in a cutaneous squamous cell carcinoma by in situ hybridization. | a case of a cutaneous squamous cell carcinoma (scc) occurring at the site of a long-standing recurrent hsv infection is described. no deficit of the cell-mediated immunity was recorded. hsv2 was isolated in several viral cultures. hsv dna was visualized in the scc by in situ hybridization with biotinylated probes in paraffin-embedded tissue. the samples were negative for hpv2, 5, 16, and 18 probes. a causal relationship between hsv infection and cutaneous scc is hypothesized. | 1989 | 2552936 |
| prevalence of antibodies to herpes simplex virus types 1 and 2 in pregnant women, and estimated rates of infection. | there has been a recent increase in notifications of genital herpes but it is not known whether this has been reflected in the pregnant population. we have therefore carried out a study to determine the prevalence of herpes simplex antibodies in pregnant women and to estimate the incidence of primary infection. sera were collected from 3533 women at antenatal clinics and tested for total antibodies to herpes simples virus (hsv), and if positive, for specific antibodies to hsv-2. estimates of hsv ... | 1989 | 2556492 |
| characterization of a continuous smooth muscle cell line derived from rabbit aorta. | a spontaneously arising continuous cell line (rb-1) derived from collagenase-elastase digested rabbit aorta has been propagated in vitro for over 100 passages. during this period, the rb-1 cells remained spindle-shaped and formed regularly oriented parallel bundles. after passage 50, rb-1 cells were found to be serum-independent in their growth and reached higher saturation density than rabbit aorta smooth muscle cells. alpha-actin and desmin filaments were detected by immunostaining in rb-1 cel ... | 1989 | 2681130 |
| proliferative t-cell response to glycoprotein b of the human herpes viruses: the influence of mhc and sequence of infection on the pattern of cross-reactivity. | oligopeptides of the highly conserved herpes virus glycoprotein b (gb) were expressed from dna fragments of the ebv gb (balf4) and hsv-2 gb open reading frames as fusion proteins with the lambda cii protein and beta-galactosidase (gz), respectively, in escherichia coli. after immunopurification using anti-gb or anti-gz affinity columns, the fusion proteins were used in vitro to stimulate human peripheral blood lymphocytes (pbl) or murine lymph node cells that have been primed with ebv, hsv-1, hs ... | 1989 | 2553584 |
| thymidine kinase deficient human cells have increased uv sensitivity in their capacity to support herpes simplex virus but normal uv sensitivity for colony formation. | a thymidine kinase deficient (tk-) and two thymidine kinase proficient (tk+) human cell lines were compared for uv sensitivity using colony-forming ability as well as their capacity to support the plaque formation of herpes simplex type 1 (hsv-1). the tk- line (143 cells) was a derivative of one of the tk+ lines (r970-5), whereas the other tk+ line (ac4 cells) was a derivative of the 143 cells obtained by transfection with purified sheared hsv-2 dna encoding the viral tk gene. 143, r970-5 and ac ... | 1989 | 2554138 |
| [malpighian epithelia infected by dna viruses and langerhans cells]. | malpighian epithelia exhibit an immune surveillance through langerhans cells (lc) against the dna viruses, which induce various epithelial lesions, mainly on the skin, conjunctiva, mouth, uterine cervix. these viral infections are usual during childhood. the most common dna viruses are: adenoviruses (more than 40 types), human papillomaviruses (hpv, more than 50 types) and herpes viruses (herpes simplex type 1 and 2, varicella and zooster, epstein-barr virus). different clinical aspects are obse ... | 1989 | 2694082 |
| anti-herpesvirus activity of citrusinine-i, a new acridone alkaloid, and related compounds. | citrusinine-i, a new acridone alkaloid isolated from the root bark of the citrus plant (rutaceae), exhibited potent activity against herpes simplex virus (hsv) type 1 and type 2 at low concentrations relative to their cytotoxicity; 50% effective concentrations (ed50) of citrusinine-i were 0.56 micrograms/ml and 0.74 micrograms/ml against hsv-1 and hsv-2, respectively. inhibitory action was also demonstrated against cytomegalovirus (cmv) and thymidine kinase-deficient or dna polymerase mutants of ... | 1989 | 2556960 |
| rapid identification and typing of herpes simplex virus by a new enzyme immunoassay with peroxidase-labeled complement c1q. | a new enzyme-linked immunoassay system (tc-elisa) was developed for the rapid, specific detection and typing of herpes simplex virus (hsv) using anti-hsv immune sera and enzyme-labeled complement component c1q (p*-c1q). the method is based on viral antigen produced in hsv-infected cells being detected by simultaneous addition of immune serum and p*-c1q (elisa-cf). with this assay, it was found that hsv was detected with anti-hsv immune serum and p*-c1q and that hsv type 1 and hsv type 2 could be ... | 1989 | 2557809 |
| herpetic stromal keratitis in congenital dysgammaglobulinemia. case report. | the clinical picture and laboratory findings of a case of secondary herpes simplex virus type 2 in a patient with congenital dysgammaglobulinemia who was followed for 26 months is described. local combined therapy of acyclovir and decadron (dexamethasone) 0.1% was given for management for 14 months. the clinical and immunopathologic findings of our patient match the results described by other investigators in experimental animals. | 1989 | 2714093 |
| gene ul11 of herpes simplex virus type 1 encodes a virion protein which is myristylated. | we have investigated whether herpes simplex virus (hsv) contains structural polypeptides which are modified by myristic acid. we demonstrate that herpes simplex virions contain a family of myristylated proteins, mr approximately 13,000 to 16,000. these were mapped, using hsv-1/hsv-2 intertypic recombinants, to 0.130 to 0.204 map units on the virus genome. using anti-peptide sera, raised against the carboxyterminus of the predicted ul11 gene product, we have established that the myristylated viri ... | 1989 | 2558153 |
| herpes simplex virus in oral mucosal ulcers in patients with hematological malignancy. | developments of oral mucosal ulcers induced by herpes simplex virus (hsv) were studied in patients with hematologic malignancy. herpes simplex virus type-1 (hsv-1) was identified by immunological staining using virus-specific monoclonal antibodies in the epithelial cells of such ulcers from two patients with malignant lymphoma (ml), three with acute myeloblastic leukemia (aml), one with refractomy anemia with excess blasts, two with chronic myelocytic leukemia (cml), one with acute lymphoblastic ... | 1989 | 2558241 |
| simian alphaherpesviruses and their relation to the human herpes simplex viruses. | biochemical and immunological properties of structural and non-structural polypeptides of the human simplex viruses (hsv1 and hsv2) and four related herpesviruses of non-human primates [herpesvirus simiae (b virus), h. cercopithicus (sa8), h. saimiri 1 (hvs 1), and h. ateles 1 (hva 1)] were compared. using a radioimmunoassay (ria), the presence of antigenic determinants shared among all six viruses was demonstrated. the relative degree of antigenic cross-reactivity among these viruses was furthe ... | 1989 | 2558632 |
| histopathologic study of herpes virus-induced retinitis in athymic balb/c mice: evidence for an immunopathogenic process. | in order to determine whether antiviral immunity is pathogenic in mouse eyes, hsv-1 was injected into the anterior chamber of one eye of adult athymic balb/c mice. the eyes of these t cell deficient mice were examined clinically and histopathologically for ocular disease. the anterior segment of injected eyes developed progressive inflammatory reactions that eventually destroyed the ciliary body and then progressed to the posterior compartment where partial necrosis occurred, but only in the inn ... | 1989 | 2558848 |
| increased efficacy of ganciclovir in combination with foscarnet against cytomegalovirus and herpes simplex virus type 2 in vitro and in vivo. | in tissue culture, efficacy against either murine cmv or hsv-2 was increased 27-fold for the acyclic nucleoside ganciclovir and 3-fold for foscarnet (trisodium phosphonoformate) when the 2 drugs were combined; whereas against human cmv, efficacy was increased 3-fold for both drugs. in mice, efficacy was increased 2-fold for ganciclovir and 4- to 5-fold for foscarnet when used in combination against either murine cmv or hsv-2. these results suggest an additive interaction between the two drugs in ... | 1989 | 2559657 |
| synthesis and biological activity of dansyl thymidines. | the synthesis of thymidines selectively dansylated in the 3'- and 5'-positions is reported. the biological investigation showed that these fluorescent nucleosides behave as competitive inhibitors of thymidine kinase (tk) from herpes simplex virus (hsv) and are endowed with a certain degree of antiviral activity against hsv-2 and hsv-1. | 1989 | 2559740 |
| t lymphocytes in genital lymph nodes protect mice from intravaginal infection with herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) is a human venereal pathogen that causes lethal neurological illness after intravaginal inoculation into balb/cj mice. intravaginal vaccination of mice with an attenuated strain of hsv-2 rapidly induces immunity to a lethal intravaginal challenge with wild-type hsv-2. this resistance is transferrable to syngeneic mice with genital lymph node (gln) cells but not with cells from other lymphoid sources. here we demonstrate that minimal numbers of hsv-2-stimulated ... | 1989 | 2783720 |
| anti-herpesvirus activity of carbocyclic oxetanocin g in vitro. | a series of new compounds, carbocyclic oxetanocins, have been synthesized and their anti-herpesvirus activity determined. carbocyclic oxetanocin g (oxt-g) was most active against herpes simplex virus (hsv) and human cytomegalovirus (hcmv) among carbocyclic oxetanocins tested; the median effective concentrations (ec50) for hsv-1, -2, and hcmv were 0.23, 0.04 and 0.40 micrograms/ml, respectively. the ec50 value of carbocyclic oxt-g against hsv-2 was significantly lower than those of acyclovir, gan ... | 1989 | 2559911 |
| [maternal genital herpes: virus isolation during the third trimester of pregnancy and at delivery]. | we followed 42 pregnant women at high risk for genital herpes infection. viral isolation was attempted weekly from the 34th week of pregnancy. one or more episodes of herpes infection developed in 74% of women. the virus was isolated in 28% after 34 weeks and in 22% after 37 weeks. a prophylactic cesarean section was performed in the latter group; only one of these women had a positive viral isolation at delivery. the hsv-2 of virus was found in 83% of cases and hsv-1 in 17%. | 1989 | 2562415 |
| hsv hepatitis in the mouse: a light and electron microscopic study with immunohistology and in situ hybridization. | in order to characterize better the morphology and immune response in acute necrotizing hsv infection, murine hsv hepatitis was examined. balb/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (pfu) of hsv-1 (lenette) and hsv-2 (d316). in both groups half the animals were pretreated with silica particles to block macrophage function. up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron ... | 1989 | 2567083 |
| lack of antiviral activity of ketoconazole alone or in combination with the acyclic nucleoside ganciclovir against a herpes virus type 2 infection in mice. | ketoconazole and ganciclovir were tested for antiviral activity, each alone and in combination, against a herpes simplex virus type 2 (hsv-2) systemic infection in swiss-webster mice. when given once daily for 5 days starting 24 hr after infection, the ed50 for ketoconazole either alone or in combination was greater than 60 mg/kg; for ganciclovir, the ed50 was 7.1 mg/kg alone and 10.8 mg/kg in combination. thus, ketoconazole did not potentiate or antagonize the antiviral activity of an acyclic n ... | 1989 | 2576599 |
| herpes simplex virus type 2 transcripts in trigeminal ganglia during acute and latent infection in mice. | adult mice were inoculated intracerebrally with herpes simplex virus type 2 (hsv-2), and perfused at days 5, 8, 81, 108, and 130 after infection. trigeminal ganglia and roots were dissected out and embedded in paraffin. four 35s-labelled dna probes that contained immediate-early (icp-0, icp-4), delayed-early (thymidine kinase; tk), or late (morphological transforming region; mtr) genes were prepared. in situ hybridization methods or an immunoperoxidase antigen method using hsv-2 antibody were ap ... | 1989 | 2592985 |
| a self-inducing runaway-replication plasmid expression system utilizing the rop protein. | a highly efficient prokaryotic expression system has been developed that produces proteins at levels exceeding 150 micrograms/ml of culture medium. the system consists of a temperature-sensitive-copy-number plasmid that carries the rop gene and promoter downstream from the trp promoter. any sequence cloned into the pvuii site of the rop gene alters rop protein activity and causes lethal runaway plasmid dna replication. this plasmid replication can be suppressed in trans by complementation with a ... | 1989 | 2670683 |
| [acyclovir and pregnancy: current aspects]. | acyclovir (acv), an antiviral nucleoside analog, is active against herpes simplex viruses (hsv1, hsv2) and varicella virus (vzv). these viruses seems to be prejudicial to the pregnant woman and to the fetus. yet, acv is not recommended for use in pregnancy. however in certain cases, this drug has been used. we review in this paper, the pharmacokinetics and transplacental passage of acv, indications, and whether the benefits of the administration of acv in pregnancy outweigh the theoretical risks ... | 1989 | 2681370 |
| a novel form of intrauterine infection by herpes simplex virus type 2: its association with spontaneous abortion, latent neonatal infection, and adult inflammatory diseases. | 1989 | 2685911 | |
| [is mollaret's meningitis caused by herpes simplex virus type 2?]. | mollaret's meningitis is characterised by recurrent, brief episodes of serous meningitis with symptom-free intervals. although viral etiology has been suspected, no pathogenic agent has hitherto been found. using the immunoblot technique, we found igg antibodies of intrathecal origin against the hsv-2-specific protein, gg2, in a patient with mollaret's meningitis. since being put on prophylactic acyclovir treatment twelve months ago, the patient has had no further episodes of meningitis. because ... | 1989 | 2709930 |
| 5-(2-bromoethyl)-2'-deoxyuridine: a selective inhibitor of herpes simplex viruses in vitro. | a new pyrimidine analog, 5-(2-bromoethyl)-2'-deoxyuridine (beudr), was tested in vitro for antiviral activity on herpes simplex virus types 1 and 2. as reference compounds, acg, bvudr and paa were used. compared to acg and bvudr, beudr resulted less potent on both hsv-1 and hsv-2. however, a 50% inhibition of the multiplication of uninfected cells could be obtained only at very high beudr concentration (id50 = 8500 microm). this makes beudr the least toxic analog known and gives it a selective i ... | 1989 | 2713989 |
| [evaluation of 2 commercial monoclonal antibody kits for the identification of herpes simplex virus]. | two commercial monoclonal antibody kits (virgo and mikrotrak for the identification of herpes simplex virus (hsv) have been assessed. in 65 positive hsv cultures from clinical samples, 25 strains (38%) were identified as hsv1 with both kits. the virgo kit identified 37 strains (57%) as hsv2, and did not identify the remaining 3 (5%). with the microtrak kit, 34 strains (52%) corresponded to type 2, 2 (3%) strains remained nonidentified, and in the remaining 4 isolates (6%) both viral types were i ... | 1989 | 2562319 |
| the inhibitory effect of gossypol on human sperm motility: relationship with time, temperature and concentration. | the inhibitory effect of gossypol acetic acid on human sperm motility was studied with a transmembrane migration method. gossypol decreased sperm motility after it had been incubated with semen for more than 15 min. however, when sperm motility was evaluated immediately after semen had been mixed with gossypol, no inhibitory effect could be found. we consider that the sperm immobilizing potency of gossypol is much less than our previously studied sperm immobilizing agents. it is unlikely that go ... | 1989 | 2714811 |
| efficacy of phosphonylmethoxyalkyl derivatives of adenine in experimental herpes simplex virus and vaccinia virus infections in vivo. | the phosphonylmethoxyalkyl derivatives (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(s)-hpmpa], 9-(2-phosphonylmethoxyethyl)adenine (pmea), and 9-(2-phosphonylmethoxyethyl)-2,6-diaminopurine (pmedap) were evaluated for their in vivo efficacies in several animal model infections, i.e., mice infected intravenously with vaccinia virus and mice infected intracutaneously, intraperitoneally, or intracerebrally with herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) or thymidine kinase-defici ... | 1989 | 2719463 |
| synthesis and antiviral activity of 5'-o-(substituted) sulfamoyl pyrimidine nucleosides. | 5'-o-[n-(aminoacyl or isobutyryl)sulfamoyl]uridines 4a-e, 5a-e and 5'-o-[n-(isopropyl)sulfamoyl]cytidines 7-9 have been synthesized and tested against herpes simplex virus type 2. condensation of 2',3'-o-isopropylidene-5'-o-sulfamoyluridine with the n-hydroxysuccinimide esters of boc-gly, boc-l-ala, boc-d-ala and boc-l-phe, gave 4a-d which, on deprotection under acidic conditions, provided 5a-d. a similar condensation of 2',3'-di-o-acetyl-5'-o-sulfamoyluridine with the n-hydroxysuccinimide ester ... | 1989 | 2730285 |
| axonal degeneration and regeneration in sensory roots in a genital herpes model. | in a mouse model of genital herpes simplex virus type 2 (hsv-2) infection, roots of the lower spinal cord were examined 5 days to 6 months after inoculation. using immunoperoxidase methods on paraffin sections, viral antigen was found in sensory ganglia, their proximal roots and distal nerves on days 5 and 6 after infection. in epon sections, most mice had focal sensory root abnormalities in lower thoracic, lumbar or sacral levels. at days 7 and 10, lesions showed chiefly nerve fiber degeneratio ... | 1989 | 2750478 |
| two cases of necrotizing myelopathy associated with malignancy caused by herpes simplex virus type 2. | two cases of necrotizing myelopathy were autopsied; one was complicated with lung carcinoma and the other with chronic type adult t cell leukemia (atl leukemia). to our knowledge, they were the first cases of their type in japan. in both cases, necrosis of the spinal cord was observed in the gray and white matter along most of its extent. marked changes were found in the lumbar segment. the patients were not treated with intravenous cancer chemotherapy or irradiation. immunohistochemical and ele ... | 1989 | 2763797 |
| antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations. | the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ... | 1989 | 2769234 |
| broad-spectrum in vivo antiviral activity of 7-thia-8-oxoguanosine, a novel immunopotentiating agent. | a novel immunopotentiating agent, 5-amino-3-beta-d-ribofuranosylthiazolo [4,5-d]pyrimidine-2,7(3h,6h)-dione (7-thia-8-oxoguanosine), lacks virus-inhibitory properties in vitro but induces interferon and potentiates immune functions, such as natural killer cell activity. it was evaluated in rodent models to determine the spectrum of antiviral activity and effective treatment regimens. at 50 to 200 mg/kg given as single or divided intraperitoneal (i.p.) doses 1 day before virus inoculation, signif ... | 1989 | 2817849 |
| effect of 2',5'-oligoadenylate on herpes simplex virus-infected cells and preventive action of 2',5'-oligoadenylate on the lethal effect of hsv-2. | antiviral effects of ppp(a2'p)na, (2-5a) on herpes simplex virus type 1 or type 2 (hsv-1 or hsv-2)-infected baby hamster kidney fibroblasts (bhk cells) and hsv-2-infected female guinea pigs were examined. 2-5a was introduced into bhk cells in the form of a calcium phosphate precipitate and as an ointment of polyethylene glycol (peg) into guinea pig vagina. under optimum conditions, 2-5a trimer and other 2-5a derivatives inhibited over 90% of hsv-1 syncytia formation and over 50% of hsv-2 plaque ... | 1989 | 2481701 |
| the effect of cimetidine on survival of mice infected with herpes simplex virus type 2, murine encephalomyelitis virus and vesicular stomatitis virus infections. | the effect of cimetidine on survival was investigated in mice infected with herpes simplex virus type 2 (hsv-2), murine encephalomyelitis virus (gd-vii), and vesicular stomatitis virus (vsv). balb/c mice, 5 weeks of age, were injected intraperitoneally (i.p.) with 5.5 x 10(5) plaque-forming units (pfu) of virus/0.5 ml, and cimetidine (1 mg/0.5 ml) was administered simultaneously. the survival rates of 80% and 85% in the cimetidine groups were significantly greater than the 10% and 23% for the co ... | 1989 | 2561403 |
| [characteristics and role of the gluteal herpes in a female population]. | based on the fact that the gluteal herpes may constitute the clinical expression of the reactivation of a herpes simplex virus latent at the sacral lymph node, we investigated a group of women who were carriers of gluteal herpetic infection, the characteristics of the infection, the virus type principally associated to it, and its possible relation with the genital herpes. forty one women with gluteal herpes verified by virologic laboratory were studied. 75.7% of these women had had in addition ... | 1989 | 2562294 |
| acyclic analogues of 3'-azido-3'-deoxythymidine as potential antiviral agents. nucleoside synthesis by michael addition. | an acyclic derivative of 3'-azido-3'-deoxythymidine, (r,s)-1-[1-(2-hydroxyethoxy)-3-azidopropyl]thymine (2), has been synthesized by a path involving michael-type addition. related thymidine analogues lacking the c(3')-c(4') bond were similarly obtained. the method provides a versatile new route to nucleoside analogues. the new compounds were found to be essentially inactive against human immunodeficiency virus type 1 (hiv-1) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro wh ... | 1989 | 2909745 |
| [viral or bacterial meningitis. differential diagnostic problems]. | a 19 year old previously healthy man presented symptoms of meningitis. culture from the cerebrospinal fluid revealed herpes simplex virus type 2 to be the responsible agent. the course was benign, the patient was never seriously ill. he recovered fully within a few weeks. the cerebrospinal fluid showed prolonged pleocytosis and low values of glucose. the traditional cerebrospinal fluid parameters employed in the diagnosis of meningitis, and for distinguishing bacterial from viral meningitis, are ... | 1989 | 2919375 |
| [herpes simplex virus infection of the central nervous system]. | herpes simplex virus type 1 (hsv 1) infection in the central nervous system results in encephalitis with high mortality and morbidity. however herpes simplex virus type 2 (hsv 2) infection in this area usually results in benign meningitis. brain biopsy is the most sensitive and most specific method for diagnosing hsv 1 encephalitis. antibody determinations in serum and cerebrospinal fluid give only a retrospective diagnosis. a rapid non-invasive diagnostic test is urgently required as an alterna ... | 1989 | 2919376 |
| a novel pharmacological approach to herpes virus infections. | 1989 | 16312792 | |
| in vitro sensitivity of clinical isolates and drug-resistant mutants of herpes simplex virus against seven antiviral drugs. | 1989 | 16312794 | |
| difference between herpes simplex virus type 1 and type 2 neonatal encephalitis in neurological outcome. | 24 infants consecutively treated with acyclovir or vidarabine for neonatal herpes simplex virus (hsv) encephalitis were followed up for 6 months to 3 years to assess neurological and developmental outcome. 15 patients had hsv-2 and 9 had hsv-1 encephalitis. infants with hsv-2 encephalitis presented with a higher frequency of seizures, greater pleocytosis and protein concentrations in the cerebrospinal fluid, and more frequent evidence of structural damage on computerised tomographic scans of the ... | 1988 | 2891886 |