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temperature effects on ph of mitochondria isolated from carp red muscle.mitochondria isolated from red muscle of carp (cyprinus carpio) were used to investigate the effects of temperature and extramitochondrial ph (phe) on the mitochondrial ph gradient and respiratory properties. mitochondria from animals acclimated to 10 degrees c were isolated and incubated in kcl-based media with 0.2 mm lauroylcarnitine (c-12) as substrate. maximal respiratory control ratios (rcr = state 3/state 4) were 16-18 between ph 6.7 and 7.4 at 10 degrees c; rcr values were 9-12 between ph ...19883354709
light and electron microscopic studies on various developmental stages of hoferellus cyprini (doflein, 1898).the development of hoferellus cyprini (myxosporea) in common carp was studied by light and electron microscopy. it has been established that in the autumn the early developmental stages are located in a cell syncytium formed from the epithelium of the renal tubules. the authors share the opinion of lom and dyková [13] that each intracellular trophozoite corresponds to a primary parasitic cell which contains secondary, tertiary and quaternary cells having formed by internal cleavage. from decembe ...198823195094
[increment of cone erg during light adaptation--carp retina (in vivo and in vitro)]. 19883414455
light-dependent dynamics of gap junctions between horizontal cells in the retina of the crucian carp.the dynamics of gap junctions between outer horizontal cells or their axon terminals in the retina of the crucian carp were investigated during light and dark adaptation by use of ultrathin-section and freeze-fracture electron microscopy. light adaptation was induced by red light, while dark adaptation took place under ambient dark conditions. the two principal findings were: (1) the density of connexons within an observed gap junction is high in dark-adapted retina, and low in light-adapted ret ...19883365755
regional difference in the dendritic morphology of dopamine cells in carp retina.the dendritic morphology of dopamine (da) cells in the inner plexiform layer of the retina of carp (body length, ca. 33 cm) was investigated by identifying their fluorescent cell bodies in isolated, aldehyde-fixed flat-mounts and injecting them iontophoretically with lucifer yellow ch under microscopic control. attention was paid to clarifying regional differences in their dendritic morphology. in the marginal zone within 0.25 mm from the retinal edge, the density of da cells was extremely high ...19883359333
characteristics of bipolar-bipolar coupling in the carp retina.on and off bipolar cells were identified in the light-adapted carp retina by means of intracellular recording and lucifer yellow dye injection. the receptive field centers, determined by measuring the response amplitudes obtained by centered spots of different diameters, were 0.3-1.0 mm for on bipolar cells and 0.3-0.4 mm for off bipolar cells. these central receptive field values were much larger than the dendritic field diameters measured by histological methods. simultaneous intracellular rec ...19883373179
adaptation of intestinal morphology in the temperature-acclimated carp, cyprinus carpio l.the effects of temperature and photoperiod acclimation upon the morphology of carp intestinal mucosa have been studied using morphometric techniques. carp intestine showed an absence of anatomical regionalisation. there was a gradual reduction in the dimensions of villi along the tract. the decrease in the dimensions of the villi was greatest in the anterior half. temperature acclimation had no effect on intestinal-somatic indices. acclimation to 10 degrees c or 30 degrees c resulted in large di ...19883345555
interaction of parvalbumin of pike ii with calcium and terbium ions.fluorimetric titrations of parvalbumin ii (pi 4.2) of pike (pike ii) with ca2+ and tb3+ show the cd and ef binding sites to be non-equivalent. the intrinsic binding constants of the strong and the weak sites obtained for ca2+ are: ksca = 1.6.10(8) m-1; kwca = 6.6.10(5) m-1. differences of the order of 100% were encountered between the tb3+ binding constants obtained with four different versions of titration. their average values are: kstb = 1.9.10(11) m-1; kwtb = 1.0.10(7) m-1. the distances of ...19883334854
protein synthesis in tissues of fed and starved carp, acclimated to different temperatures.the temperature dependence of the rates of protein synthesis in the red and white skeletal muscle of the carp (cyprinus carpio) was measured using a method which involved a single injection of tritiated phenylalanine. plasma and muscle-free phenylalanine quickly reached a plateau level at all temperatures. during the plateau phase the incorporation of label into protein was liner. muscle from fish previously acclimated to either a low temperature (8°c) or a high temperature (28°c), showed marked ...198824226298
comparison of effects of paraquat and methidation on enzyme activity and tissue necrosis of carp, following exposure to the pesticides singly or in combination.under aquarium conditions, treatment with the herbicide paraquat (pq) and with the insecticide methidation (md) caused cell damage and stress in carp (cyprinus carpio l.), as shown by increases in glutamate-dehydrogenase (gidh, ec 1.4.1.2), glutamate-oxalacetate transaminase (got, ec 2.6.1.1) and lactate dehydrogenase (ldh, ec 1.1.1.27) activities and in blood sugar levels. pq proved synergistic with md in certain cases as regards the harmful effect exerted. on combined treatment, dilated extrac ...198815092508
isolation of types i and v collagens from carp muscle.1. the major constituent of carp intramuscular connective tissue was found to be type i collagen. 2. a collagen homologous to type v collagen of higher vertebrate was also isolated from carp muscle. 3. relative portion of type v collagen was higher in carp muscle than in mammalian muscles.19883396321
[a case of acute renal failure after eating the raw gallbladder of a carp]. 19883373090
[reaction of the carp cyprinus carpio l. at different periods of its ontogeny to the action of organophosphorus compounds].effect of some organic phosphorous compounds on the carp cyprinus carpio l. in consecutive stages of its development has been studied. the least and most vulnerable stages in carp's development under the action of these compounds have been identified. the gravity of disorders in fish organism on early stages and the degree of pathological changes induced by organic phosphorous compounds have been shown. embryotoxic and gonadotoxic effect of these compounds has been found.19883358968
a study of the hydration and thermodynamics of warm-water and cold-water fish collagens.the hydrated volumes, vh, of collagens extracted from various fish species were calculated by using the simha-einstein equation, and it was found that the hydration of warm-water fish collagen is greater than that of cold-water fish collagen (halibut). although the intrinsic viscosities of warm-water fish (bigeye-tuna, carp and catfish) collagens are almost the same, the hydrated volume of bigeye-tuna collagen is approx. 1.5 and 3 times those of carp and catfish collagens respectively. the exten ...19883342002
gabaergic inhibition on dopamine cells of the fish retina: a [3h]dopamine release study with isolated fractions.inner retinal cells including dopamine (da) cells were isolated and fractionated from the carp (cyprinus carpio) retina by an enzyme cell dissociation and metrizamide gradient centrifugation method. when gamma-aminobutyric acid (gaba) antagonists (bicuculline and picrotoxin) were added into the perfusate over such a cell fraction, they stimulated the release of [3h]da which had been preloaded in the cell fraction. the action of gaba antagonists was dose and ca2+ dependent. their minimal effectiv ...19883335837
effects of [d-arg6, trp7, leu8, pro9net]-luteinizing hormone-releasing hormone (sgnrh-a) and [d-ala6, pro9net]-luteinizing hormone-releasing hormone (lhrh-a), in combination with pimozide or domperidone, on gonadotropin release and ovulation in the chinese loach and common carp.the effects of lhrh-a and sgnrh-a alone and in combination with the dopamine receptor antagonists pimozide (pim) and domperidone (dom) on stimulation of gonadotropin (gth) secretion and ovulation in chinese loach (paramisgurnus dabryanus) and common carp (cyprinus carpio) were studied. when tested in the absence of a dopamine receptor antagonist, sgnrh-a was about 10-fold more potent than lhrh-a in stimulating gth secretion in loach, whereas these peptides were of similar effectiveness in common ...19883282976
[study on ldh isoenzymes of hybrid yue-carp and its parents]. 19883273660
effects of various oxygen concentrations on antioxidant enzymes and the quantity of tissue phospholipid fatty acids in the carp.analyses were made of the phsopholipid fatty acids and the antioxidant enzymes in the carp (cyprinus carpio morpha) at three different oxygen concentrations, corresponding to hyperoxia, hypoxia and anoxia. variations of the oxygen concentration were found to influence the quantities of phsopholipid fatty acids, as well as the antioxidant enzyme activities. in hyperoxia and hypoxia the amount of polyunsaturated fatty acids in carp liver was higher than in anoxia, but in other tissues there was no ...19883254009
comparative histochemistry of the mucoproteic cells of the hypophysis from rhamdia hilarii, hypostomus punctatus, prochilodus scrofa and cyprinus carpio (teleostei). immunohistochemical identification of the gonadotropic cells.adenohypophyseal cells showing positive histochemical reactions for mucosubstances were classified as type i-iv in hypostomus (plecostomus) punctatus (loricariidae), rhamdia hilarii (pimelolidae), prochilodus scrofa (prochilodontidae) and cyprinus carpio (cyprinidae) according to cell shape, size, cytological characteristics and adenohypophyseal distribution. cell types i and ii are common to the four species, with each cell type showing very similar cytological and histochemical characteristics ...19883242625
effects of dopamine on spatial properties of horizontal cell responses in the carp retina. 19883231368
[rhythmic activity of the olfactory bulb of the carp under nembutal anesthesia].the effect of nembutal intraperitoneal injections upon the induced waves and orthodromic potentials of the olfactory bulb (ob) induced by odor stimulation and paired electric stimuli of the olfactory nerve was examined in immobilized by diplacine carp (cyprinus carpio l.). the nembutal anesthesia resulted in a gradual decrease in frequency of induced waves tested by orthodromic response and amplitude of spontaneous electric activity ob ob. the changes found under nembutal anesthesia in evoked el ...19883219368
ultrastructural analysis of leucocyte interaction with tumour targets in a teleost, cyprinus carpio l.the first step in natural killer activity, target cell binding, was investigated ultrastructurally in order to identify presumptive cytotoxic cells. after incubation of unfractioned peripheral blood cells of the carp, cyprinus carpio l., with hela tumour cells as targets, regular conjugate forming was observed. targets were surrounded by cells bearing features either of lymphocyte or monocyte/macrophage types. cells with the usual lymphocytic characteristics contained acid phosphatase positive g ...19883208960
isolation and partial characterization of igm-like immunoglobulins from the serum of carp (cyprinus carpio l.), frog (rana ridibunda pall.) and tortoise (testudo graeca pall.).1. igm-like immunoglobulin fractions have been isolated by combinations of precipitation procedures and gel-filtration from the serum of three vertebrate species, representative of the classes of pisces (cyprinus carpio l.), amphibia (rana ridibunda pall.) and reptilia (testudo graeca pall.). 2. the homogeneity of the isolated fractions has been confirmed electrophoretically and immunoelectrophoretically. 3. electrophoretic mobility of igm, similar for the three species studied has been observed ...19883132346
the effects of reserpine and lhrh or salmon gnrh analogues on gonadotropin release, ovulation and spermiation in common carp (cyprinus carpio l.).the effects of reserpine (catecholamine depletor) and lhrh analogues on gonadotropin secretion, spermiation and ovulation of common carp were investigated. injections of reserpine alone at a dose of 1 or 7 mg/kg of body weight stimulated spermiation, and reserpine at a dose of 1 mg/kg of body weight in combination with (d-arg6, trp7, leu8, pro9, net)-lhrh (s-gnrh-a) or with (d-ala6, pro9)-lhrh (lhrh-a) at a dose of 50 micrograms/kg of body weight caused an increase of plasma gonadotropin levels, ...19883072624
calcium action potentials in retinal cells of the carp. 19883068589
electrocardiograms in five bipolar leads recorded from the body surface of three fish species (cyprinus carpio, oreochromis niloticus and pagrus major) in fresh or sea-water.1. ecgs were recorded using five bipolar leads from the body surface of porgy pagrus major and tilapia oreochromis niloticus in fresh or sea-water, or held on a dry towel. 2. the maximum qrs amplitude was detected in lead v in both species being 54 +/- 25 microv in porgy and 241 +/- 78 microv in tilapia, respectively, when the fish were held on the dry towel. 3. clear ecg waves could not be obtained from porgy in sea-water because of the porgy's small cardiac potential and leakage of the potenti ...19882907442
effects of a high concentration of co2 on electrocardiograms in the carp, cyprinus carpio.1. the effects of a high concentration of co2 (pco2 = 250 mmhg and po2 = 360 mmhg in water) and ms222 (tricaine methanesulfonate, 1/8000 or 1/5000) on the electrocardiogram (ecg) in carp were examined using five kinds of bipolar leads from the body surface. 2. in the carp anesthetized with the high concentration of co2 for 30 min, the qrs duration, pq interval and and direction of the qrs axis on the frontal plane significantly changed. even after recovery from anesthesia, delay in the qrs durat ...19882907441
effects of metal ions on the antioxidant enzyme activities, protein contents and lipid peroxidation of carp tissues.1. studies were performed regarding the effects of cuso4 in concentrations of 5, 10, 25 and 50 ppm and znso4 in concentrations of 10 and 100 ppm on the antioxidant enzyme activities, lipid peroxidation and protein contents of tissues of common carp (cyprinus carpio morpha l.) exposed to these pollutants for 24 hr. 2. the results demonstrated that cuso4 was more toxic than znso4 and that both treatments brought about significant changes in these parameters in carp hepatopancreas (liver), gill and ...19882904875
the effect of isoprenaline on the phospholipid content of the compact and spongious musculature of the carp ventricular myocardium.1. after a single injection of 40 mg kg-1 of isoprenaline to the carp, lysophospholipids appear in the tissue of the heart ventricle, ethanolamine plasmalogens increase and choline plasmalogens decrease; phosphatidylinositol is lowered in the spongious layer only. 2. daily administration of 5 mg kg-1 of the drug leads, after 5 doses, to a dramatic decrease of the diphosphatidylglycerol content; during the subsequent 5 and 10 doses a return to normal values occurs. shifts in plasmalogens are simi ...19882904868
[effect of baclofen and muscimol on the evoked potentials of the tectum mesencephali in the carp in response to stimulation of the visual and somatosensory afferent inputs].the perfusion of (+/-) 10(-5) m baclofen to the optic tectum of the carp greatly reduced evoked potential to the optic nerve stimulation and slightly inhibited evoked potential to the spinal cord stimulation. the perfusion of 10(-4) m muscimol reduced the former evoked potential as well as the latter one. the results permit supposing that tectal visual input is more sensitive to the presynaptic inhibitory influences through gabab-receptors than the somatosensory afferent input.19882854884
identification of hypocalcin (teleocalcin) isolated from trout stannius corpuscles.we have isolated and purified a glycoprotein from the corpuscles of stannius (cs) of trout, which we consider hypocalcin (also called teleocalcin), the major hypocalcemic hormone of fish. this product is present in relatively large amounts in the cs of several species (i.e., european eel, tilapia, goldfish, and carp). hypocalcin is typically released from the cs in response to an experimentally induced increase of the blood calcium concentration. ultrastructural observations show that after this ...19883360288
morphology of physiologically identified mitral cells in the carp olfactory bulb: a light microscopic study after intracellular staining with horseradish peroxidase.physiologically identified mitral cells in the carp olfactory bulb were stained by intracellular injection of horseradish peroxidase in order to study the morphology in detail. the somata were fusiform, elongated, oval, triangular, or irregular. the mean diameters of the somata were 30 microns x 14 microns. two to five thick dendrites arose from the somata and frequently gave off branches to form glomerular tufts. the dendrites extended less than 400 microns; the dendritic field of single mitral ...19883343400
[characterization of opioid receptor from bullfrog brain].among the tested five species--carp, bullfrog chicken, rat, and mouse--the solubilized fraction derived from the bullfrog brain gave the highest binding activity similar to that of membranes. the difference in their binding activities was demonstrated to depend on the bmax value, but not on kd. high-affinity binding of [3h]naloxone and [3h]ethylketocyclazocine (ekc) was proved in supernatant of detergent-treated membranes. the displacement curves of various [3h]ligands binding to the membrane an ...19882851236
amino acid sequence and three-dimensional structure of cytosolic adenylate kinase from carp muscle.the amino acid sequence of cytosolic adenylate kinase from carp muscle has been determined. for the analysis of the blocked amino terminus an n-acylaminoacyl peptidase has been isolated and applied successfully. the resulting sequence is homologous to those of other adenylate kinases. it has 76% and 74% identical amino acids when compared with the enzymes from chicken and pig, respectively. the carp enzyme forms x-ray-grade crystals, the structure of which has been solved at 0.58-nm resolution. ...19882851785
effect of manganese ions on the immune response of carp (cyprinus carpio) against yersinia ruckeri.the effect of manganese ions mn2+ (mn cl2) on hematocrit and the antibody response of carp (cyprinus carpio) was investigated after exposure of fish to contaminated water for 2.5 months. contamination of water with 50 mg/l mn2+ did not decreased antibody production against yersinia ruckeri (measured by agglutination) but significantly decreased hematocrit. the levels of manganese in lymphoid organs (spleen and kidney) increased progressively during the experiment. the effect of manganese in vitr ...19883169354
dna:dna reassociation analysis of aeromonas salmonicida.dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ...19883171541
comparative biotransformation of molinate (ordram) in the white sturgeon (acipenser transmontanus) and common carp (cyprinus carpio).1. juvenile white sturgeon (acipenser transmontanus) and common carp (cyprinus carpio) were exposed to 100 micrograms/l [ring-14c]molinate in a flow-through metabolism system. exposure was in three phases: acclimation (2 h), uptake (24 h), and depuration (24 h). 2. excreted metabolites were collected on a macroreticular resin, and retained metabolites were extracted from homogenized fish tissue. identification and quantification was by h.p.l.c. cochromatography (gradient conditions) and determin ...19883176521
[the seasonal dynamics of sphaerospora renicola (myxosporidia) in carp (osteichthyes)].we observed 2 different types of the course of incidence of sporogonic phase and blood phase. type i shows in both phases a maximum in the first summer, a minimum in winter or autumn and a second maximum between late autumn and second summer. in this case the sporulation was especially distinct with the first maximum. type ii starts in the first summer with the blood phase without occurrence of the sporogonic phase (and the swim-bladder phase), which appears only from autumn until the second sum ...19883177937
carp gamma-crystallins with high methionine content: cloning and sequencing of the complementary dna.the nucleotide sequences of gamma-crystallin cdnas cloned from the common carp (cyprinus carpio) have been determined. the amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for gamma-m1 and gamma-m2, respectively. they exhibit unusually high methionine contents: 12.4% for gamma-m1 and 14% for gamma-m2. comparison of both fish gamma-crystallins with bovine gamma-ii crystallin reveals that they are similar in structure. the striking features of both fish ...19883191133
the complete amino acid sequences of beta-subunits of two distinct chum salmon gths.the complete amino acid sequences of beta-subunits of chum salmon (oncorhynchus keta) gonadotropin (gth) i and gth ii were determined. both gth i beta and gth ii beta were treated with neuraminidase, and then reduced and carboxymethylated. both of the modified subunits were digested separately with several endopeptidases. the resulting fragments were purified by reversed-phase high-performance liquid chromatography and subjected to sequence analysis by manual edman degradation. gth i beta was a ...19883192067
interactions between human and carp (cyprimus carpio) low density lipoproteins (ldl) and ldl receptors.1. we have compared the concentration and chemical composition of carp and human plasma lipoproteins and studied their interaction with human fibroblast ldl receptors. 2. the main lipoproteins in carp are of high density (hdl) in contrast to low density lipoproteins (ldl) in human. 3. carp lipoproteins are devoid of apolipoprotein (apo) e, a major ligand for interaction with ldl receptors in mammals. 4. carp very low density lipoproteins (vldl) and ldl but not hdl nor apoa-i cross react with hum ...19883197401
daily cycles in plasma melatonin levels under long or short photoperiod in the common carp, cyprinus carpio.a radioimmunoassay (ria) for plasma melatonin (mlt) was simplified for use with the common carp (cyprinus carpio). plasma was partially purified with sep-pak c18 cartridge before ria. the inhibition curves for the sep-pak c18 fraction from the plasma of carp, goldfish (carassius auratus), yellow tail (seriola quinqueradiata), japanese eel (anguilla japonica), bora (mugil cephalus cephalus), kisu (sillago japonica), ishigarei (kareius bicoloratus), and shimaisaki (rhyncopelates oxyrhynchus) were ...19883197949
dopamine cells and rod bipolar cells contain protein kinase c-like immunoreactivity in some vertebrate retinas.the localization of cells immunoreactive to a monoclonal antibody against protein kinase c (pkc) and to polyclonal antibodies against tyrosine hydroxylase (th) was investigated in the retina of fish (carp, goldfish, dace and catfish), frog, turtle, chick and some mammalians (guinea pig, rat, cat and rabbit) by means of fluorescence microscopy. pkc-like immunoreactivity was found in dopamine (da) or th-like immunoreactive (ir) cells in all the species examined and also in rod bipolar cells in the ...19883205402
seasonal variations of glucose and triiodothyronine concentrations in serum of carp (cyprinus carpio l).thyroid hormone and glucose serum concentrations (sc) of carps (cyprinus carpio l) have been monitored at weekly intervals throughout the year. t4 and rt3 concentrations were always below the limit of detection of the assays applied. t3 and glucose mean sc showed seasonal variations. highest t3 sc were reached in summer with a peak value in august and lowest values in winter with a maximum in december. glucose sc have been found to be highest in winter and lowest in summer. statistical analysis ...19883220447
a comparative study of aldehyde dehydrogenase and alcohol dehydrogenase activities in crucian carp and three other vertebrates: apparent adaptations to ethanol production.in the final step of the pathway producing ethanol in anoxic crucian carp (carassius carassius l.), acetaldehyde is reduced to ethanol by alcohol dehydrogenase. the presence of aldehyde dehydrogenase in the tissues responsible for ethanol production could cause an undesired oxidation of acetaldehyde to acetate coupled with a reduction of nad+ to nadh. moreover, acetaldehyde could competitively inhibit the oxidation of reactive biogenic aldehydes. in the present study, the distribution of aldehyd ...19883220990
trace element residues in bluegills and common carp from the lower san joaquin river, california, and its tributaries.whole-body samples of bluegills (lepomis macrochirus) and common carp (cyprinus carpio) from the san joaquin river and two tributaries (merced river and salt slough) were analyzed to determine if the concentrations of any of nine elements were elevated as a result of exposure of the fish to agricultural subsurface (tile) drainage water. highest concentrations (micrograms/g dry weight) detected were as follows (first number in each pair applies to bluegills and the second to carp): arsenic (as), ...19883222692
[residues of active substances after consumption of contaminated food: status report on the evaluation of residues with examples of two drugs].pharmacokinetic models are presented for the computation of time courses of blood levels of drugs in man following the consumption of contaminated food. mathematically, two linear systems of differential equations are set up for the donor organism (e.g., trout) and for the recipient, (e.g., man), where the first system generates the initial conditions for the second. models of this kind are applied to the transfer of chloramphenicol to man via carp and trout (which had previously been administer ...19883223091
purification and some properties of glyceraldehyde-3-phosphate dehydrogenase from carp muscle.glyceraldehyde-3-phosphate dehydrogenase was purified from carp white muscle. on cm-sephadex chromatography two well separated active peaks were obtained. both of them show a single protein band on gel electrophoresis and have the same molecular and kinetic properties; they differ only by the amount of bound nad, the enzyme in the second peak being coenzyme-free. significant differences were observed between the properties of carp and pig muscle enzymes. glyceraldehyde-3-phosphate dehydrogenase ...19883232465
xenobiotic metabolizing enzyme systems in test fish. iii. comparative studies of liver cytosolic glutathione s-transferases.the reduced glutathione (gsh)-conjugating capacities of the livers of trout, carp, zebra fish, guppy, and bluegill have been evaluated with several substrates preferentially metabolized by the major gsh-transferase isozymes. carp and zebra fish lack gsh-transferase activity with 1,2-epoxy-3-(p-nitrophenoxy)propane. guppy is characterized by the highest activities with 1-chloro-2,4-dinitrobenzene and the absence of activity toward trans-4-phenyl-3-buten-2-one. enzyme activities with the other sub ...19883234293
a comparative trial of garlic, its extract and ammonium-potassium tartrate as anthelmintics in carp.while fish chemotherapy is not a common practice in méxico, folk medicine is usually well accepted by fish farmers. empirical knowledge suggested that garlic should have anthelmintic properties. various trials were carried out in cyprinus carpio infested with capillaria sp., using minced garlic and its extracts. an empirical concentration of 200 mg/l of minced garlic showed the greatest activity against the above mentioned worms (100%). the hexane extract in equivalent amounts showed a 75% effec ...19883253490
the protein sequence homology of gamma-crystallins among major vertebrate classes and their dna sequence homology to heat-shock protein genes.a systematic characterization of lens crystallins from five major classes of vertebrates was carried out by exclusion gel filtration, cation-exchange chromatography and n-terminal sequence determination. all crystallin fractions except that of gamma-crystallin were found to be n-terminally blocked. gamma-crystallin is present in major classes of vertebrates except the bird, showing none, or decreased amounts, of this protein in chicken and duck lenses, respectively. n-terminal sequence analysis ...19883255376
comparative studies on the structure of biliary immunoglobulins of some avian species. ii. antigenic properties of the biliary immunoglobulins of chicken, turkey, duck and goose.studies on the immunological cross-reactivity of the igs+ from the bile of chicken and turkey (order galliformes) as well as duck and goose (order anseriformes) to igs with antigenic known properties carried out by a series of ria. various class-specific anti-ig sera produced in rabbits, guinea-pigs and mice were used. the following results were obtained: (1) a high extent of antigenic relationship between the chicken and turkey biliary igs was demonstrated by a radiobinding system consisting of ...19883260198
adaptational change in cone-mediated electroretinogram in human and carp. 19883265993
the fetal phenotype of the 18p-syndrome. report of a male fetus at twenty-one weeks.morphological and cytogenetic findings in a male fetus at 21 weeks gestation after prenatally detected monosomy 18p are reported. the fetus displayed dysmorphic features resembling the 18p-syndrome, such as decreased head circumference, slightly receding forehead, hypertelorism, epicanthus, horizontal palpebral fissures, depressed nasal bridge, long philtrum, carp mouth, irregular crenated maxillar alveolar ridge, retrognathia, lowset dysplastic ears with posterior rotation, edema of neck, hands ...19883281573
contractile response to substance p in isolated smooth muscle strips from the intestinal bulb of the carp (cyprinus carpio).1. the effect of substance p on the mechanical activity of carp intestinal bulb smooth muscle was investigated in vitro. 2. bath-applied substance p (1 nm-1 microm) caused concentration-dependent contraction of the smooth muscle. the ec50 value was 20 +/- 3 nm (n = 13). 3. pretreatment with tetrodotoxin (780 nm) or atropine (500 nm) partially decreased the contractile response to substance p, while methysergide (3 microm) did not decrease the response. 4. the contractile response to substance p ...19882455618
antigenic determinants on human choriogonadotropin alpha-subunit. ii. immunochemical mapping by a monoclonal antipeptide antibody.in order to study antigenic site(s) present in the carboxyl-terminal part of the alpha-subunit of human choriogonadotropin (hcg-alpha), we attempted to produce site-specific antibodies directed against a 34-residue synthetic peptide analogous to region 59-92 of hcg-alpha. from a fusion experiment performed with a mouse injected with hcg-alpha-(59-92)-peptide conjugated to tetanus toxoid as immunogen, we selected a monoclonal antipeptide antibody (designated fa36) which has high binding activity ...19882455715
effect of pesticides (atrazine and lindane) on the replication of spring viremia of carp virus in vitro.atrazine and lindane are widely employed as pesticides in agriculture, which by wash off, could contaminate the aquatic environment. in this study, they were tested in vitro on the replication of the virus of spring viremia of carp (svc) in epithelioma papulosum cyprini cell line. they were dissolved first in dmso and then in medium at their solubility limit in water (28 and 10 mg/l). the results of the kinetic titers after 72 h showed no statistically significant difference between control and ...19882461139
the immune response of carp against dextran. an analysis of the idiotype heterogeneity.the expression of a "species-specific" idiotype with specificity for alpha(1-6) dextran in carp is described. all tested carp anti-dextran antisera react with a rabbit anti-id (carp anti-dex) serum. the rabbit anti-id serum can be partially inhibited by the specific ligand, dextran t70 or isomaltodecaose but not or only weak with other, unrelated carbohydrates. these data confirm our statement, that a limited germline v-gene repertoire may code for antibodies in fish.19882463185
presence of a substance p-like peptide in an acid extract of the intestinal bulb of the carp (cyprinus carpio).1. the effect of an acid extract of the carp intestinal bulb (eci) on guinea-pig ileum longitudinal smooth muscle (gplm) and carp intestinal bulb longitudinal smooth muscle (ciblm) was examined. 2. eci caused a concentration-dependent contraction of gplm and ciblm. this eci-induced response was reduced by atropine to 30-40% of the control, indicating that part of the contracting activity of eci is attributable to acetylcholine. the atropine-resistant contracting activity of eci was not mediated ...19882464388
anti-dextran antibodies of carp detected by the enzyme-linked immunosorbent assay (elisa).carp produce anti-alpha (1-6) dextran antibodies after immunization with a vaccine of leuconostoc mesenteroides b512. these antibodies can be determined by passive haemagglutination, quantitative precipitation or by elisa. in our hands the elisa has proved to be 100 times more sensitive than the passive haemagglutination test. we could not found any correlation between the anti-dextran activity estimated with the passive haemagglutination on the one and the elisa on the other hand.19882465680
properties of odour-binding glycoproteins from rat olfactory epithelium.the specific membrane glycoproteins with high affinity for camphor and decanal were isolated from rat olfactory epithelium. antibodies to these glycoproteins inhibited both the electroolfactogram and the binding of odorants. the enzyme immunoassay has shown these glycoproteins to be present in the olfactory epithelium of rat, mouse, guinea-pig and hamster but not in that of frog and carp. the molecular mass of the odour-binding glycoproteins from rat olfactory epithelium solubilized by triton x- ...19883337807
cutaneous tumor of northern pike, esox lucius l. ii. expression of surface and cytoplasmic immunoglobulins and alpha-naphthyl acetate esterase by mononuclear cells from lymphoid tissues and tumor.rabbit anti-pike igm antibodies showed that up to 95% of mononuclear (mn) cells isolated on ficoll-isopaque gradients from peripheral blood, spleen, and head kidney of northern pikes carrying cutaneous tumors were surface (sig) and cytoplasmic (cig) immunoglobulin positive by indirect immunofluorescence. on the contrary, rabbit anti-winter flounder igm antibodies gave totally negative results. monoclonal anti-carp igm antibodies stained approximately 40-56% of peripheral blood, splenic, and head ...19883350193
species differences in 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and biotransformation in fish.rainbow trout, yellow perch, carp, bluegill, largemouth bass, and bullhead were treated with graded doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd; 1, 5, 25, or 125 micrograms/kg) or vehicle, ip. the lethal potency of tcdd tended to be greater in yellow perch, carp, and bullhead than in the other three species (ld50 80 days post-treatment, 3-5 versus 10-16 micrograms/kg, respectively). all species treated with the highest dose of tcdd (125 micrograms/kg) displayed a latency period of 1-4 wee ...19883356307
gonadotropin content of the pituitary gland of gonadal tumor-bearing common carp x goldfish hybrids from the great lakes, as assessed by bioassay and radioimmunoassay.the hybrids of carp (cyprinus carpio) and goldfish (carassius auratus) collected from the lower great lakes between 1978 and 1981 exhibited epizootics of gonadal neoplasm which were rare in the parental species. the pituitary glands of hybrids were, on average, 2.5 times larger than that of carp of similar body size. histologically, the hypertrophy was due to hyperplasia of proximal pars distalis basophils (the presumptive gonadotropes). using a carp gonadotropin (gth) radioimmunoassay it was fo ...19883366360
isolation and properties of the pituitary gonadotropin from silver carp (hypophthalmichthys molitrix).the gonadotropin of silver carp (scgth) was purified from the pituitary glands in a yield of 8.4 mg/g through extraction with 6% ammonium acetate (ph 5.1)-40% ethanol followed by 80% ethanol precipitation, deae-cellulose chromatography and gel filtration. the molecular weight of scgth is about 32,000 as estimated by gel filtration. leucine and serine were the two n-termini of scgth. the amino acid composition of scgth is rich in aspartic acid/asparagine, glutamic acid/glutamine, valine, proline ...19883366548
monoclonal antibodies directed against the calcium binding protein parvalbumin.we have produced twelve monoclonal antibodies (mcab) against carp-ii parvalbumin. three of them, designated 235, 239, 267 recognize determinants conserved in fish, chicken, mouse, rat, monkey and human parvalbumin. we show their use in the qualitative detection of parvalbumin (pv) by immunohistochemistry, in the quantitation of parvalbumin by radioimmunoassay and in the detection of parvalbumin on immunoblots.19883383226
carp, a novel gene regulating the transcription of the carbamoylphosphate synthetase operon of escherichia coli.the carab operon, encoding carbamoylphosphate synthetase (cpsase; ec 6.3.5.5) is transcribed from two tandem promoters. the upstream promoter (p1) is controlled by pyrimidines and the downstream promoter (p2) is controlled by arginine. we have isolated a new type of constitutive mutation (carp) that specifically affects the control of the pyrimidine-sensitive promoter but does not appear to influence other genes of the pyrimidine pathway. the carp mutation acts in trans and is dominant, which su ...19883065518
identification and immunocytochemical localization of two different carbonic anhydrase isoenzymes in teleostean fish erythrocytes and gill epithelia.carbonic anhydrase was purified from the gills (cab) of the rainbow trout salmo gairdneri and from erythrocytes (cae) of the fresh water carp cyprinus carpio. the purification of the isozymes was confirmed by sds acrylamide gel electrophoresis. antibodies against the purified cab and cae were then raised in rabbits. specificity was verified by immunoblotting. no cross-reaction was found between them, using the immunodot technique. cab antiserum was used to specifically localize gill ca in the tr ...19883139588
nucleotide sequence of chicken myb proto-oncogene promoter region: detection of an evolutionarily conserved element.the nucleotide sequence of the chicken myb proto-oncogene putative promoter region was determined and compared with the corresponding sequence of the mouse c-myb gene (1). 118 bp upstream from the initiation codon suggested by gerondakis and bishop (2) for the chicken c-myb protein, a 124-bp-long conserved element was found (92% identity in chicken and mouse sequences). sequences homologous to this element were detected on southern blots of restricted genomic dnas from mouse, man, lizard, frog, ...19883145493
scrapie-associated fibrils, prp protein and the sinc gene.scrapie-associated fibrils (saf) are disease-specific structures found in extracts of the brains of animals affected with scrapie. these structures are pathological aggregates of a normal host protein called prp. in collaboration with konrad beyreuther (heidelberg), we have characterized the multiple forms of prp found in saf fractions from mouse brain affected by the me7 strain of scrapie. there is no in vivo n-terminal cleavage of the most abundant forms of prp. however, n-terminal cleavage of ...19882900718
republic act no. 6657, 10 june 1988.this act institutes the comprehensive agrarian reform program (carp). carp takes as its declared aim "the establishment of owner cultivatorship of economic-size farms as the basis of philippine agriculture" (section 2). it prohibits the retention by landowners of more than five hectares of land and, for each child of the landowner over 15 years old, an additional three hectares, providing he or she is personally cultivating that land (section 6). that limit of three hectares is also the upper ...198812289467
[larvicidal action of liquid nitrogen against metacercariae of clonorchis sinensis]the author investigated the larvicidal action of liquid nitrogen against the metacercariae of clonorchis sinensis, with an observation on the freshness and change of taste in the flesh of fishes. the results obtained were as follows: the metacercariae in the flesh of pseudorasbora parva, which were treated with liquid nitrogen (-192c), were not killed within 10 seconds, but completely killed over 30 seconds. in comparison, the metacercariae in the flesh of p. parva kept in a refrigerator (-12c) ...198712886062
[studies on the metagonimus fluke in the daecheong reservoir and the upper stream of geum river, korea]the prevalences of the fluke belonging to genus metagonimus have been reported along the upper stream of inhabitants by several workers since 1980, however the taxonomical problems of the fluke was not yet settled. the larval flukes; cercaria and metacercaria as well as their intermediate hosts, and adult were studied in order to identify the metagonimus in the areas. the results obtained are summarized as follows: the snails, semisulcospira globus were collected from the three different localit ...198712886084
yersinia enterocolitica, yersinia frederiksenii and yersinia intermedia in cyprinus carpio (linneo 1758). 19873591113
homeothermic fish and hemoglobin: primary structure of the hemoglobin from bluefin tuna (thunnus thynnus, scromboidei).some fish are warm-bodied, e.g. the bluefin tuna (thunnus thynnus), which has a muscle temperature 12-17 degrees c higher than its environment. this endothermy is achieved by aerobic metabolism and conserved by means of a heat-exchanger system. the hemoglobins of bluefin tuna are adapted to these conditions by their endothermic oxygenation, thus contributing to the preservation of the body energy. this is a new and so far unique property of tuna hemoglobin. the primary structure of the alpha and ...19873620111
intrinsic fluorescence of carp hemoglobin: a study of the r----t transition.the intrinsic fluorescence of hemoglobins is known to respond to ligand-induced changes in the quaternary structure of the protein. carp hemoglobin is an interesting model to study the quaternary transition since its r----t equilibrium is ph-dependent and at low ph, in the presence of organic phosphate, it remains in the t or 'deoxy' quaternary structure, even when saturated with ligand. in this study, using front-face fluorometry, we show that the intrinsic fluorescence intensity exhibited by c ...19873620472
the influence of environmental temperature on the lymphocyte populations in carp (cyprinus carpio l.).the influence of environmental temperature upon the lymphocyte populations in carp (cyprinus carpio l.) has been studied by immunoperoxidase labeling using different antisera. in carps kept in a warm and a cool environment, respectively, rabbit anti-carp igm as well as rabbit anti-carp fc antiserum showed different percentages of ig-positive lymphocytes, whereas the percentage remained unaffected by temperature after the use of rabbit anti-carp fab antiserum with a specificity against l-chains. ...19873555478
ultrastructural characterization of leucocytes in the pronephros of carp (cyprinus carpio, l.).in the pronephros of carp (cyprinus carpio, l.) the following cells were found and ultrastructurally characterized: erythrocytes; lymphocytes and plasma cells; thrombocytes; neutrophilic, eosinophilic and basophilic granulocytes; phagocytic reticular cells and monocytes and non-phagocytic reticular cells. the cells could be separated on a percoll continuous density gradient and relatively pure fractions could be obtained. in vitro phagocytosis of bacteria (bacillus megaterium) was found in monoc ...19873109968
increased gonadotropin levels in goldfish do not result in alterations in circulating thyroid hormone levels.to determine whether increases in gonadotropin levels are capable of altering thyroid function in goldfish, plasma thyroid hormone levels were measured following induced changes in endogenous gonadotropin secretion and injection of carp gonadotropin. radio-frequency lesions placed in the nucleus preopticus periventricularis or monosodium-l-glutamate-induced lesions of the posterior nucleus lateralis tuberis (nlt) of the hypothalamus were capable of stimulating significant increases in plasma gon ...19873114043
xenobiotic-metabolizing enzyme systems in test fish. i. comparative studies of liver microsomal monooxygenases.all monooxygenase activities assayed in guppy (poecilia reticulata) and red killifish (oryzias latipes) were 7-10 times higher than those measured in trout (salmo gairdneri), carp (cyprinus carpio), and golden orfe (leuciscus idus). cytochrome p-450 was 3 times higher in the former fish than in all the other species. zebra fish (brachydanio rerio) 7-ethoxycoumarin o-deethylase and bluegill (lepomis macrochirus) ethylmorphine n-demethylase (emnd) were not greatly different from those of the forme ...19873830019
experimental verification of the effect of the form (scaly or scaleless) of carp host (c. carpio l.) on the growth rate of micropopulations of gyrodactylus katharineri malmberg, 1964 (monogenea).it was demonstrated that the growth rate of gyrodactylus katharineri micropopulations parasitizing the fry of cyprinus carpio l. was effected by water temperature and by the form of the fish host (scaly or scaleless). at lower water temperature (12 degrees c) the number of parasites increased in both host forms approximately at the same intensity. in warmer water (18 degrees c), the parasites reproduced intensively only on the scaly form of fish, whereas no parasites were found on the scaleless ...19873428761
interaction of mycoplasma mobile 163k with erythrocytes.mycoplasma (m.) mobile 163k isolated from fish was investigated for its hemadsorbing, hemagglutinating, and hemolysing capacities and for its ability to adhere to erythrocytes. hemadsorption to the colonies of m. mobile occurred with ovine, bovine, equine, trout, and carp erythrocytes and was inhibited by treatment of the mycoplasmas with substances acting on proteins (pronase, trypsin, glutaraldehyde), heat, uv-irradiation and homologous antiserum. hemadsorption could be prevented also by treat ...19873439386
environmental changes induced by growth-associated triggering factors in injured optic nerve of adult rabbit.central nervous system (cns) neurons of mammals regenerate poorly after axonal injury. however, if an injured cns neuron (rabbit optic nerve) is supplied with appropriate soluble substances ("growth-associated triggering factors") derived from medium conditioned by regenerating fish optic nerve or newborn rabbit optic nerve, it can express regeneration-associated characteristics. such characteristics include a general increase in protein synthesis, changes in synthesis of specific polypeptides, ...19873470808
transferability of immune plasma and pronephric cells in isogeneic, allogeneic and xenogeneic transfer systems in crucian carp.transfer of immunity to horse erythrocytes (hrbc) by intravascular injection of immune plasma and pronephric cells was performed using histocompatible and histoincompatible crucian carp to examine "mhc" regulation in adoptive immunity. clonal ginbuna crucian carp, carassius gibelio langsdorfii and outbred kinbuna crucian carp, c. carassius buergeri immunized with hrbc were used as donors of plasma and cells for transfer to unimmunized members. recipients were then evaluated by measuring the anti ...19872960574
contribution to the knowledge of gyrodactylus gurleyi price, 1937 (monogenea: gyrodactylidae).gyrodactylus longoacuminatus zitnan, 1964, forma minor sensu ergens (1985) was identified as g. gurleyi price, 1937. morphological and metrical evaluations of the hard parts of opisthaptor of more than 200 specimens provided data on the variability of this parasite; cyprinus carpio haematopterus represents its new host. since the shape of the hard parts of opisthaptor of g. gurleyi is very similar to that of g. longoacuminatus, further detailed studies of the two species will be necessary, parti ...19873666611
first record of sphaerospora renicola dyková et lom, 1982 and myxobolus encephalicus mulsow, 1911, pathogenic protozoans of carp from the u.s.s.r. 19873666614
immune response of carps (cyprinus carpio l.) against dextran.in carps no antibodies could be evoked by immunization with soluble high molecular weight dextran using doses of 1-5000 mg dextran per kg body weight. on the other hand, an immune response against dextran could be elicited by immunization with a vaccine of leuconostoc mesenteroides b512 as well as a dextran-bsa-conjugate. these carp anti-dextran antibodies showed precipitating and agglutinating activities with specificity against alpha linked dextran determined by the almost complete inhibition ...19872439389
mechanism of the interaction between myelin basic protein and the myelin membrane; the role of arginine methylation.the addition of solutions of bovine myelin basic protein to suspensions of unilamellar vesicles prepared from whole myelin suspensions results in the rapid equilibrium association of the vesicles into dimers, followed by time-dependent aggregation reactions. other cationic proteins also induce the dimerization of the vesicles and equilibrium constants for dimer formation are obtained for bovine myelin basic protein, lysozyme, polyhistidine and myelin basic protein from carp, which differs from t ...19872440426
effect of gamma-hexachlorocyclohexane (lindane) on carp (cyprinus carpio). i. effect of chronic intoxication on humoral immunity in relation to tissue pollutant levels.lindane, the gamma-isomer of hexachlorocyclohexane, one of the most widely used insecticides, was incorporated into carp food for 109 days. the effects of 10, 100, or 1000 ppm of lindane in food on hematocrits and on antibody production against yersinia ruckeri bacterin in sera and mucus were followed for 79 days. no effect of lindane on these parameters and on spleen weight was observed. lymphoid organs, spleen and kidney, were as highly contaminated at the beginning of the inoculation (day 30) ...19872440660
oxygen bonding in human hemoglobin and its isolated subunits: a xanes study.the x-ray absorption near edge structure (xanes) spectra of the human adult and foetal hemoglobin, of the isolated alpha and beta chains, in the oxygenated forms, and of the oxymyoglobin and carp oxyhemoglobin have been measured at the wiggler beam line of the frascati synchrotron radiation facility. the bonding angle of oxygen molecule at the iron site in these hemoproteins in solution, has been measured using the multiple scattering theory for data analysis.19872443133
sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies.from 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached sepharose 4b. the antibodies bound to the agarose matrix were eluted with 0.5 m d-galactose containing buffers. using species-specific anti-immunoglobulin sera in immunoelectrophoresis, the immunoglobulin character of the protein fractions prepared was a ...19872444220
carp (cyprinus carpio) heart has a high sensitivity to the positive inotropic effect of strophanthidin despite negative force-frequency relationships.1. the relationship between response of the heart to increased stimulation frequency and digitalis sensitivity was examined comparing the positive inotropic effect of strophanthidin and [3h]ouabain binding to sarcolemmal na+, k+-activated adenosine triphosphatase (na+, k+-atpase) in carp heart, which showed a negative force-frequency relationship, and in guinea-pig heart, which has a positive relationship. 2. in ventricular muscle preparations isolated from carp heart, strophanthidin increased d ...19872822523
cloning and sequencing of a carp beta s-crystallin cdna.the mrnas were extracted from common carp (cyprinus carpio) lenses, purified, reverse transcribed, dc tailed and cloned into escherichia coli with pbr322 as vector. the cloning efficiency was around 1 x 10(7) colonies per micrograms of mrna. a clone (pc20) was found by hybrid-arrested to contain the cdna related to carp crystallins. however, comparison of the derived amino-acid sequence with bovine gamma-ii and beta s-crystallins indicates that this carp crystallin sequence resembles closely the ...19873307924
primate specific sialoglycoprotein of sperm head plasma membrane defined by an anti-carbohydrate monoclonal antibody.a sialoglycoprotein, an integral component of the head plasma membrane of human spermatozoa, is recognized by the a-hs 1a.1 monoclonal antibody. the antigenicity is associated with the sugar moiety since: a) trypsin digestion did not affect the antigenic determinant; b) pretreatment of the cells with beta-glucosidase, alpha-mannosidase and neuraminidase completely abolished antibody binding. endoglycosidase d and glycopeptidase f were inactive. the a-hs 1a.1 did not recognize a variety of blood- ...19873317219
[sphaerospora renicola (myxosporidia), the agent of protozoal swim bladder inflammation in young carp (renicola sphaerosporosis)]. 19873328529
carp maturational-ovulatory gonadotropin but not carp vitellogenic gonadotropin or salmon maturational-ovulatory gonadotropin stimulates testosterone production by rat leydig cells in vitro.carp (cyprinus carpio) maturational-ovulatory gonadotropin, prepared from the fraction of pituitary extract adsorbed on con a-sepharose (con a ii) and subsequently adsorbed on cm-cellulose (whatman cm-52), stimulated testosterone production by isolated rat leydig cells. the fraction of carp pituitary extract unadsorbed on the immobilized lectin (con a i) with a mol. wt of 30,000, which had previously been shown to contain vitellogenic gonadotropin, was devoid of steroidogenic activity. salmon (o ...19872881654
the interplexiform-horizontal cell system of the fish retina: effects of dopamine, light stimulation and time in the dark.interplexiform cells contact cone horizontal cells in the fish retina and probably release dopamine at synaptic sites. the effects of dopamine, certain related compounds, and light and dark régimes were tested on the intracellularly recorded activity of horizontal cells in the superfused carp retina to elucidate the functional role of the interplexiform cell. dopamine application onto retinae kept in the dark for 30-40 min increased the size of the responses of cone horizontal cells to small-spo ...19872888119
metabolic activation of 2-aminofluorene, 2-acetylaminofluorene and n-hydroxy-acetylaminofluorene to bacterial mutagens with mussel (mytilus galloprovincialis) and carp (cyprinus carpio) subcellular preparations.1. the mode of activation of 2-aminofluorene (af), 2-acetylaminofluorene (aaf) and n-hydroxy-acetylaminofluorene (oh-aaf) to salmonella typhimurium ta 98 mutagens was investigated in subcellular fractions from the digestive gland of the mussel mytilus galloprovincialis and from the liver of carp cyprinus carpio. 2. in carp liver microsomes the activation of oh-aaf was due to very active deacetylase, in contrast to undetectable deacetylase-dependent activation in mussel microsomes. 3. af and aaf ...19872890481
a d-mannose-specific lectin from gerardia savaglia that inhibits nucleocytoplasmic transport of mrna.a new lectin has been isolated from the coral gerardia savaglia by affinity chromatography, using locust gum as an absorbent, and d-mannose as eluant. final purification was achieved by bio-gel p300 gel filtration. the agglutinin is a protein composed of two polypeptide chains with a mr of 14800; the two subunits are not linked by disulfide bond(s). the isoelectric point is 4.8, the amino acid composition is rich in the acidic amino acids aspartic acid and glutamic acid. the absorption maximum f ...19872890521
effect of polarisation of horizontal cells on the on-centre bipolar cell of carp retina. 1987213734
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