Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| occurrence of human papillomavirus type 16 dna sequences and c-myc oncogene alterations in uterine-cervix carcinoma. | using genetic engineering and molecular biology techniques, we have examined sixteen human carcinomas in the uterine-cervix tumors (the most frequent tumor in méxico, representing 34% of malignant tumors in women), for the presence of human papillomavirus type 16 (hpv-16) dna sequences and possible alterations of the cellular myc (c-myc) proto-oncogene. in this study we have analyzed cervical carcinomas from patient with clinical stage ii. we detected in 31% of these samples, the presence of hpv ... | 1989 | 2562235 |
| high rate of human papillomavirus type 16 infection in cytologically normal cervices. | 1989 | 2563804 | |
| relation between infection with a subtype of hpv16 and cervical neoplasia. | a variant (hpv16b) of the previously described human papillomavirus (hpv)16 (now called hpv16a) has been identified by polymerase chain reaction assays. compared with hpv16a, hpv16b has a 21bp deletion and several point mutations within the upstream regulatory region. all cervical carcinoma samples studied contained only hpv16a. hpv16b was found only rarely in cervical neoplasia but was common in the normal population. | 1989 | 2566780 |
| human papillomavirus and cervical cancer in indian women. | 1989 | 2573776 | |
| retraction: human papillomavirus subtype 16b. | 1989 | 2574815 | |
| [risk factors for condylomata. a case control study]. | the present study involves 214 patients whose cervical smear showed signs of human papilloma virus infection (hpv), matched with 1042 controls. we demonstrate increasing odds ratio with the total number of sexual partners since first sexual intercourse (p less than 0.0001). moreover the cases have more often changed partners in the year preceding the diagnosis (odds ratio 2.6 p less than 0.0001). their male partners more often have occupations that expose them to "extra-conjugal adventures" (p = ... | 1989 | 2600372 |
| vulvodynia. a multifactorial clinical problem. | symptomatic vulvar burning (vulvodynia) in the absence of abnormal physical findings was long thought to be an unusual psychosomatic gynecologic problem. within the past decade, however, a number of investigators began to study patients with this frustrating problem. initial physician insistence on a major role for psychological factors has gradually given way to sophisticated searches for evidence of persistent infectious agents, especially human papillomavirus and candida. gynecologists search ... | 1989 | 2643930 |
| [assessment of human papilloma virus colposcopic findings of the cervix (in the italian colposcopic classification]. | hpv-induced flat condylomatosis is not only much more frequently observed on the hexocervix than the florid type, but it also induces colposcopic changes in the epithelium without completely altering its morphology. hpv-induced changes have now been largely identified and are recognisable in colposcopy. they can therefore be classified in the various patterns that characterise colposcopically the transformation epithelium, with undeniable advantages not only from the point of view of their corre ... | 1989 | 2671807 |
| perspectives on research and diseases of the tropics: an asian view. | diarrhea, pneumonia, and malnutrition account for most of mortality and morbidity in children in developing countries. the expanded program of immunization (epi) is making progress with more than 50% of children under the age of 1 year receiving vaccination against the 6 epi-listed diseases. the eradication of poliomyelitis by 2000 is realistic, so that the world could be smallpox- and polio-free by the 21st century. in july-august 1988 a cholera epidemic erupted in delhi, india in which sever ... | 1989 | 2698093 |
| cervical intraepithelial neoplasia in lymphoma patients: a cytological and colposcopic study. | twenty-seven patients with hodgkin's (n = 19) and non-hodgkin's (n = 8) lymphomas underwent cytological and colposcopic screening of the uterine cervix. colposcopically directed cervical punch biopsies were taken from all patients in whom a colposcopic abnormality was detected. lymphoma patients were compared with 79 controls with normal cervical cytology and no known haematological abnormality. colposcopically directed punch biopsies were taken from the cervical transformation zone of all contr ... | 1989 | 2713244 |
| [infection by the human immunodeficiency virus (hiv) in french guyana. dermato-venereologic problems]. | dermato-venereal manifestations in hiv infection and its severe evolution stage, aids, is of particular importance in tropical zones: we may be suspicious of the viral infection and consequently to request serologic tests to confirm it. we get an explanation of the virus transmission during heterosexual relations by the frequent occurrence and importance of the genital manifestations, leading to consider aids as a true sexually transmitted disease. beside the classical opportunistic infections, ... | 1989 | 2725241 |
| cervical cytology in a community laboratory. | a retrospective analysis of the results of 27,801 cervical smears from one year in a community laboratory was undertaken. this represented 23,820 individual patients. abnormalities were seen in 9.3% of patients. three point seven percent of patients had benign abnormalities, 5.5% had cervical intraepithelial neoplasia and 0.1% had major abnormalities. the incidence of cervical epithelial abnormality was highest in the 15-34 age group but major abnormalities were seen most often in the 55+ age gr ... | 1989 | 2739985 |
| malignant transformation of anorectal giant condyloma acuminatum (buschke-loewenstein tumor). | giant condyloma acuminatum, originally described by buschke and loewenstein in 1925 as a lesion of the penis, is more rarely seen in the anorectum and is characterized by clinical malignancy in the face of histologic benignity; however, malignant transformation to frankly invasive squamous-cell carcinoma has been described. malignant transformation has been reported in 15 patients with "ordinary" condylomata acuminata as well. twenty giant condylomata acuminata have been previously reported, six ... | 1989 | 2791784 |
| detection of human papillomavirus type 11 dna in a conjunctival squamous cell papilloma by in situ hybridization with biotinylated probes. | a 44-year-old man presented with an exophytic papillomatous growth in the tarsal conjunctiva of his right eye. the tumour was excised and subjected to histological examination. on light microscopy, a squamous cell papilloma without signs of dysplasia was disclosed, because the entire tissue block was cut into sections, the performance of a conventional in situ hybridization for detection of human papillomavirus (hpv) dna was not possible. an alternative approach was used to demonstrate the prese ... | 1989 | 2801046 |
| sexually transmitted pathogens in pregnant women in a rural south african community. | one hundred and ninety three consecutive pregnant women attending peripheral antenatal clinics attached to ngwelezana hospital, empangeni, kwa-zulu, were examined for evidence of sexually transmitted pathogens. the following incidences were found: trichomonas vaginalis 49.2% (95), candida spp 38.3% (74), chlamydia trachomatis 11.4% (22), gardnerella vaginalis 6.2% (12), neisseria gonorrhoeae 5.7% (11), positive syphilis serology results 11.9% (23), hepatitis b surface antigen 4.1% (eight). no wo ... | 1989 | 2807289 |
| spatula/cytobrush vs. spatula/cotton swab detection of cervical condylomatous lesions. | human papillomavirus is widely implicated as a primary etiologic factor in cervical carcinoma. this study compared the sampling accuracy of the modified ayre spatula/zelsmyr cytobrush versus the modified ayre spatula/cotton swab in the detection of cervical lesions with koilocytotic features. the histologic categories consisted of koilocytotic lesions (flat condylomas) with minimal cellular atypia and cervical intraepithelial neoplasia (cin) i, ii and iii with surface koilocytes showing cellular ... | 1989 | 2810246 |
| [oral contraceptives and cancer]. | recently some studies have shown an association between the pill and increased risk of breast cancer and cancer of the cervix. several well- designed prospective studies indicated that there was an increased risk of breast cancer for women without children with menarche before 13 who took the pill for many years. a control case study of 407 breast cancer victims compared 424 controls found that the risk of women who had taken hormonal contraceptives was double, however, the duration of use, ag ... | 1989 | 2814116 |
| human papilloma virus infections. | genital warts are believed to be caused by human papilloma viruses and to be sexually transmitted. the viruses are classified by dna types, which appear to cause different types of disease. the choice of treatment, and usually its success rate, vary according to the type of disease and its location. | 1989 | 21248973 |
| human papillomavirus types and localization in adenocarcinoma and adenosquamous carcinoma of the uterine cervix: a study by in situ dna hybridization. | formalin-fixed, paraffin-embedded tissues from 108 cases of invasive carcinoma of the uterine cervix, consisting of 40 cases of adenocarcinoma, 44 cases of adenosquamous carcinoma, and, as a control, 24 cases of squamous cell carcinoma were examined for the presence of human papillomavirus (hpv) dna by in situ hybridization of high sensitivity using tritium-labeled hpv-2, hpv-6, hpv-16, and hpv-18 dna probes. this method detects five genome copies of homologous hpv dna per cell. hpv dna was dete ... | 1988 | 2827890 |
| humoral and cellular immunity to papillomavirus in patients with cervical dysplasia. | the cell-mediated and humoral immune responses to human papillomavirus (hpv) were tested in groups of patients with various degrees of cervical intraepithelial neoplasia (cin) using a lymphocyte proliferation assay (lpa) as a measure of circulating sensitised t-cells and an enzyme-linked immunosorbent assay (elisa) for antibodies. twenty-three of 92 patients (25%) gave stimulation indices (s.i.) greater than two to at least one of the several antigen preparations tested in the lpa. of 282 patien ... | 1988 | 2828535 |
| human papillomavirus and cervical cancer. | 1988 | 2895274 | |
| effect of cigarette smoking on cervical epithelial immunity: a mechanism for neoplastic change? | langerhans' cells in cervical epithelium in colposcopic biopsy specimens were identified by immunocytochemical staining for s100 protein and t6 (cd1) antigen, and their density was quantified. possible cofactors for the development of cervical neoplasia were examined for any effect on the cell counts per unit area. current cigarette smoking was associated with a significant decrease in the langerhans' cell population in both normal epithelium and lesions due to cervical intraepithelial neoplasia ... | 1988 | 2901519 |
| absence of cytomegalovirus, epstein-barr virus, and papillomavirus dna from adenoma and adenocarcinoma of the colon. | biopsy specimens from 13 patients with adenocarcinoma of the colon and from 10 patients with endoscopic polypectomies for colon adenoma were examined for the presence of the dna of cytomegalovirus (cmv), epstein-barr virus (ebv), and human papillomavirus (hpv) types 2, 6, 16 and 18. the specific activities of viral dna probes obtained by nick--translation ranged from 10(7) to 10(8) cpm/micrograms dna. by southern blot hybridization with an estimated sensitivity of 10 pg virus dna which correspon ... | 1988 | 2903634 |
| [study of immunologic parameters in parenteral drug addicts (pda) with condylomata acuminata]. | a study is made of the state of cell and humoral immunity in parenteral drug addicts both with and without genital warts. the results reveal alterations in the lymphocyte subpopulations, with a decrease in the t-helper/t-suppressor (t4+/t8+) ratio of both groups of patients. these alterations were more pronounced in those patients with genital warts (p less than 0.05). the alterations in humoral immunity, expressed as a hypergammaglobulinemia igg and igm, were similar in both groups. none of the ... | 1988 | 2904516 |
| future trends in cervical cancer. | the identification of the close association of certain types of human papillomavirus with the development of cervical cancer should lead to an extensive revision of appropriate health policies. having taken into account the drawbacks inherent in the existing data (stemming from the use of varying nomenclature, diagnostic methods and reliability, registration and screening practices) it is possible to conclude that the incidence of hpv infections, all premalignant and malignant stages of cervical ... | 1988 | 2841018 |
| epithelial alterations adjacent to 111 vulvar carcinomas. | material from 111 invasive primary vulvar carcinomas was reviewed in order to study the histopathologic changes adjacent to the neoplasia. the histopathologic characteristics of the adjacent tissue were divided into categories. dystrophic lesions were adjacent to invasive cancer in 57.6% of the cases, carcinoma in situ (cis) in 21.6% and epithelial changes suggestive of human papillomavirus infection in 18.9%. a spectrum of epithelial changes, ranging from hyperplastic dystrophy without atypia t ... | 1988 | 2841458 |
| intraepithelial and invasive neoplasia of the vulva in association with human papillomavirus infection. | colposcopic examination of 335 women with cytologically detected human papillomavirus (hpv) revealed involvement of the cervix in 316 patients (94%), vagina in 276 (82%) and vulva in 148 (44%). a symptom complex of pruritus and superficial dyspareunia was found in 98 of the 148 patients with vulvar infection (66%). histologic examination revealed hpv-associated vulvar intraepithelial neoplasia (vin) in 11 of 148 biopsies (7.4%). follow-up of the patients with hpv infection with or without vin sh ... | 1988 | 2841459 |
| human papillomavirus-16 and genital cancer: are tests for the viral gene expression in vitro indicators for risk factors in vivo? | human papillomaviruses are found in up to 90% of all cervical carcinomas and are considered to play a causal role in the etiology of this malignancy. the genome of human papillomaviruses consists of a single circular dna molecule with a size of approximately 8000 basepairs. 90% of this genome encodes proteins involved in functions such as neoplastic transformation of the host cell or formation of the viral capsid. the remaining 10% of the genome, which is termed upstream regulatory region (urr), ... | 1988 | 2841899 |
| involvement of human papillomavirus type 8 genes e6 and e7 in transformation and replication. | we investigated the transforming activity of human papillomavirus type 8 (hpv8) by expressing all early open reading frames from a heterologous promoter in different rodent fibroblast lines. morphological transformation was observed only in g418-selected mouse c127 and rat 1 cells containing an intact e6-coding region. e6 of hpv8 did not transform nih 3t3 cells as did e6 of bovine papillomavirus type 1. c127 cells transformed by e6 were anchorage independent and had a reduced serum requirement b ... | 1988 | 2843666 |
| interplay of viral and cellular proteins along the long control region of human papillomavirus type 18. | the long control region of human genital papillomavirus type 18 harbors transcription regulatory elements, such as the e6 promoter and a cell type-specific enhancer independent of the e2 protein. by performing dnase i footprint experiments in vitro with protein extracts from different cell lines and tissues we searched for cellular factors interacting with the totality of the control region. we detected a total of eight different protected sites; most of them were found with all the extracts. tw ... | 1988 | 2845145 |
| high prevalence of genital tract papillomavirus infection in female adolescents. | to investigate clinical condyloma, abnormal cervical cytologic findings, and evidence of human papillomavirus infections, 89 adolescent girls were examined. cellular dnas extracted from exfoliated cervical cells were examined for human papillomavirus genomic sequences by southern transfer hybridization using 32p-labeled human papillomavirus dna probes. human papillomavirus sequences were detected in 12 (13%) young women, abnormal cytologic specimens in 21 (24%), and vulvar condylomas in 12 (13%) ... | 1988 | 2845351 |
| two human papillomavirus dnas molecularly cloned from a patient with epidermodysplasia verruciformis: restriction maps. | two distinct human papillomavirus (hpv) dnas (my-1 and my-2) were molecularly cloned from the benign skin lesions of a patient with epidermodysplasia verruciformis. the restriction map of my-1 was the same as that of hpv 3a. the map of my-2 appeared to be different from those of any hpvs reported in the literature. my-2 did not cross-hybridize with my-1 or the dnas of hpv types 1, 2 and 4 under stringent conditions. | 1988 | 2845712 |
| morphometric assessment of the biological potential of human papillomavirus infections in the uterine cervix. | a series of 69 punch biopsies derived from 513 women prospectively followed up for cervical human papillomavirus (hpv) infections (including hpv lesions with and without cervical intraepithelial neoplasia; hpv-cin, hpv-ncin), and 42 control cases (consisting of normal epithelia, and classical cin lesions) were analyzed morphometrically, using a semiautomatic image analyzer (ibs i-kontron), to assess the value of morphometric measurements in discriminating between hpv lesions and cin, and to find ... | 1988 | 2846018 |
| paget's disease of the vulva: search for herpes simplex virus antigens and human papillomavirus antigen and dna. | specimens from three women with paget's disease of the vulva were tested for presence of herpes simplex virus type 2 (hsv2) antigens and human papillomavirus (hpv) antigen and dna. in one of the lesions, the hsv2-associated antigen icsp 34/35 was demonstrated. neither hpv antigens nor hpv dna were detected in the lesions. | 1988 | 2846413 |
| characterization of papillomavirus polypeptides from bovine cutaneous fibropapillomas. | virions of bovine papilloma virus (bpv) were isolated from a pool of cutaneous bovine fibropapillomas and purified by cscl gradient centrifugation. sds-page revealed several polypeptides with an mr ranging from 76k to 19k. western blot analysis of the viral isolate identified additional polypeptides when a rabbit anti-bpv serum was used, but only the main capsid component of 57k when a rabbit antiserum raised against human papillomavirus was used. the viral preparation was then 125i-labelled and ... | 1988 | 2846766 |
| induction of anchorage-independent growth of human embryonic fibroblasts with a deletion in the short arm of chromosome 11 by human papillomavirus type 16 dna. | human embryonic fibroblasts with a large deletion (11p11.11p15.1) in the short arm of one chromosome 11 (del-11 cells) appeared to be susceptible to transformation by early human papillomavirus type 16 (hpv-16) dna, whereas diploid human embryonic fibroblasts were not. this difference in susceptibility might be explained by the absence of a tumor suppressor gene located within the deleted part on the short arm of chromosome 11. the presence of abundant viral early-gene transcripts in transformed ... | 1988 | 2846869 |
| comparison of formalin, buffered formalin, and bouin's fixation on the detection of human papillomavirus deoxyribonucleic acid from genital lesions. | detection of nucleic acid sequences homologous to human papillomavirus (hpv) relies primarily on their extraction from unfixed tissue. we detected hpv sequences in dna extracted from paraffin-embedded tissue fixed in formalin (buffered and unbuffered) and bouin's solution by dot blot hybridization. a detectable hybridization signal was noted in 32% of these fixed tissues which were chosen from cases where hpv dna was detected in the unfixed tissue. when using a homologous 32p-labeled probe and a ... | 1988 | 2846966 |
| glucocorticoid-dependent oncogenic transformation by type 16 but not type 11 human papilloma virus dna. | squamous cell carcinoma of the uterine cervix is one of the most common cancers among women. correlation between human papilloma virus (hpv) infection of the uterine cervix and the development of cervical neoplasia has been established. more recent studies have shown the presence and expression of integrated hpv types 16 and 18 dna sequences in 70-80% of cervical tumours and tumour cell lines. it has been suggested that, in addition to hpvs, other agents such as hormones and tobacco products act ... | 1988 | 2847052 |
| [condylomata acuminata and similar disorders caused by human papillomavirus; clinical and immunological aspects]. | 1988 | 2847063 | |
| penile human papillomavirus infection in consorts of women with genital human papillomavirus infection. a pilot study. | a study determined the incidence of human papillomavirus (hpv) on genitalia of male consorts of women with histologically proven genital hpv lesions to be 50%. | 1988 | 2847335 |
| presence of human papillomavirus type 16 genome in bladder carcinoma in situ of a patient with mild immunodeficiency. | using blot hybridization, we analyzed 10 bladder tumors (1 transitional cell carcinoma in situ, 1 adenocarcinoma, and 8 papillary tumors) for the presence of human papillomavirus (hpv) dna. we detected hpv 16 dna in a transitional cell carcinoma in situ, whereas no hpv dna was found in the other bladder tumors. the patient, a 40-year-old female, who harbored hpv 16 dna in the bladder tumor, had mild immunodeficiency and recently suffered from the bladder tumor, common warts on the right hand, bo ... | 1988 | 2847865 |
| squamous papillary neoplasia of the adult upper aerodigestive tract. | selected papillary squamous tumors of the upper aerodigestive tract (uadt) mucosa in adult patients do not have well-defined histologic criteria and the clinical behavior is poorly understood. to better characterize this spectrum of neoplasms, uadt papillary neoplasms were evaluated by routine histology, determination of cellular dna content using feulgen-stained tissue sections, and the typing of human papillomavirus (hpv) by in situ hybridization. solitary papillomas were studied in two patien ... | 1988 | 2847971 |
| subclinical human papillomavirus infections in male sexual partners of female carriers. | the male sexual partners of 156 women with human papillomavirus infection of the cervix uteri were examined. in 120 men (77 per cent) penile lesions were found on examination of the penis via a colposcope (peniscopy) the most common of which were flat acetowhite lesions (53 per cent). predilection sites of lesions were the urethral meatus and the corona glandis (23 and 19 per cent of the lesions, respectively). using filter in situ hybridization human papillomavirus-deoxyribonucleic acid was fou ... | 1988 | 2848137 |
| human papillomavirus type 16 cooperates with activated ras and fos oncogenes in the hormone-dependent transformation of primary mouse cells. | the effect of human papillomavirus (hpv) and activated oncogenes on the growth and morphology of primary baby mouse kidney (bmk) cells has been studied. early region dna from hpv types 16, 18, 31, and 33, but not type 6, under the transcriptional control of a heterologous, retroviral promoter cooperated with ej-ras to produce cell lines that gave rise to carcinomas in syngeneic immunocompetent animals. the same hpv constructs, when cotransfected with a plasmid containing sequences from the finke ... | 1988 | 2848240 |
| human papillomavirus & herpes simplex virus type 2 dna sequences with chronic cervicitis & invasive cervical cancer. | 1988 | 2848763 | |
| detection of ebv and hpv dna sequences in oral "hairy" leukoplakia by in situ hybridization. | oral "hairy" leukoplakia (ohl) is a white lesion of the oral mucosa, usually located on the lateral tongue among human immunodeficiency virus (hiv) positive acquired immunodeficiency syndrome (aids)-risk patients. the lesion has been reported to be associated with epstein-barr virus (ebv) and human papillomavirus (hpv). twenty surgical biopsy specimens were evaluated for the presence of hpv genus-specific antigen, hpv 2/4, 6/11, and 16/18 dna and ebv dna by in situ hybridization employing formal ... | 1988 | 2849634 |
| [screening by colposcope of partners of women with genital human papillomavirus infection]. | 1988 | 2850515 | |
| cervical human papilloma virus infection and colposcopy. | 1988 | 2850790 | |
| intraepithelial neoplasia, human papilloma virus infection and argyrophilic nucleoprotein in cervical epithelium. | a silver colloid technique was applied to 50 colposcopic biopsies of cervix. these comprised nine cases of wart virus infection of the cervix, 11 cases of cervical intraepithelial neoplasia (cin) i, nine cases of cin ii, eight cases of cin iii, seven normal biopsies and six cases showing only incomplete squamous metaplasia. the mean numbers of argyrophilic nucleolar organizer regions (agnors) increased from cin i to cin iii. statistically significant differences for agnors were found in comparis ... | 1988 | 2850991 |
| cytological screening to detect subclinical anal human papillomavirus (hpv) infection in homosexual men attending genitourinary medicine clinic. | unselected homosexual men attending a department of genitourinary medicine were screened for human papilloma virus (hpv) infection using anal cytology. satisfactory smears were obtained from 221 patients, and 73 showed cytological abnormality with warty atypia. abnormal cytology was detected in 55 (31%) of 178 patients in whom there was no macroscopic evidence of anal or perianal warts, and anal cytology may therefore be valuable to detect patients with subclinical condylomatous lesions and may ... | 1988 | 2852158 |
| the e7 open reading frame of human papillomavirus type 16 encodes a transforming gene. | prior analysis of bovine papillomavirus has identified two genes, e5 and e6, which induce morphologic transformation of certain established cells. to study the transforming genes of human papillomavirus (hpv) type 16, the hpv type most commonly associated with cervical carcinoma, we examined subgenomic viral dnas under control of a retroviral long terminal repeat for their capacity to induce cellular transformation of nih 3t3 cells. plasmids carrying the entire viral early region, the e6 and e7 ... | 1988 | 2852339 |
| [detection of human papillomavirus in a cervix precancerous condition. initial report]. | case report of a 21 years old woman with cervical, vaginal and vulvar condyloma. we have demonstrated the presence of human papilloma viruses (hpv) with help of in-situ-hybridisation. | 1988 | 2852422 |
| [detection of human papilloma virus dna in cervical carcinoma cells in chinese patients]. | 1988 | 2852556 | |
| detection of human papillomavirus dna in anogenital exophytic lesions by in situ hybridization to paraffin sections. | in situ hybridization was used to detect human papillomavirus (hpv) nucleic acids (type 6b, 11, 16, 18, 31, and 33) and immunohistochemistry was done to detect papillomavirus common antigen in paraffin sections of biopsy specimens. two patients suffering from condyloma acuminatum contained hpv-6b and hpv-11. both cases showed small foci of the antigen-positive cells. one patient having condyloma acuminatum with dysplastic features contained a small quantity of hpv-16 without any antigen-positive ... | 1988 | 2853930 |
| the epidemiology of human papillomavirus infection in relation to cervical cancer. | 1988 | 2853999 | |
| the natural history of early cervical neoplasia and cervical human papillomavirus infection. | this article deals with the natural history of cervical human papillomavirus infection and precancer. it traces the development of the transformation zone and maturation of the normal squamous epithelium. potential aetiological agents and a possible cofactor are briefly discussed. the natural history of premalignant disease and its relationship to invasive disease is also covered. | 1988 | 2854002 |
| human papilloma virus induced epidermal proliferations of the anogenital region. | 1988 | 2854969 | |
| [detection of human papillomavirus sequences in uterine cervix lesions in venezuela]. | 1988 | 2855187 | |
| sensitive and quick test for human papilloma virus. | 1988 | 2856324 | |
| [cervical intraepithelial neoplasm and human papilloma virus]. | 1988 | 2856453 | |
| accumulation of rna homologous to human papillomavirus type 16 open reading frames in genital precancers. | the accumulation of human papillomavirus type 16 (hpv-16)-specific rnas in tissue sections from biopsies of patients with genital precancers was studied by in situ hybridization with single-stranded 35s-labeled rna. these analyses revealed that the most abundant early-region rnas were derived from the e4 and e5 open reading frames (orfs). rnas homologous to the e6/e7 orfs were also detected, whereas rnas homologous to the intervening e1 orf were not. this suggests that the e4 and e5 mrnas are de ... | 1988 | 2824859 |
| cervical lesions in patients with malignant melanoma. | koilocytes were found in the cervical epithelium in association with cervical intra-epithelial neoplasia in 8 out of 9 cases of malignant melanoma. this suggests that the cervical lesion in them was of viral origin. in 62 women with malignant melanoma but without cervical atypia there was also an excess of koilocytosis compared with controls with neither lesion. these findings point to the possibility that human papilloma virus infection may also be involved in the development of malignant melan ... | 1988 | 2453994 |
| condyloma acuminatum of the bladder in two autopsy cases. | condyloma acuminatum is a very rare lesion occurring in the bladder. we report two cases of condyloma acuminatum of the bladder found at autopsy, one in a 96-year-old woman and the other in an 80-year-old man. both had papillary or polypoid tumorous lesions in the trigone and neck of the bladder. the lesions were composed of nodular or papillary growths of squamous epithelium. the epithelium exhibited koilocytosis and the nuclei showed immunohistochemical staining for human papillomavirus antige ... | 1988 | 2455958 |
| characterization of normal human exocervical epithelial cells immortalized in vitro by papillomavirus types 16 and 18 dna. | an in vitro system for studying the interaction between human papillomavirus (hpv) 16 and 18 recombinant dna and normal human exocervical epithelial cells is described. eight hpv-immortalized human exocervical epithelial cell lines were established; all the lines contained either integrated hpv16 or 18 sequences and expressed hpv mrnas. thus, integration and expression appear to be required for immortalization. immortalized cells (greater than 200 population doublings to date) divided rapidly (d ... | 1988 | 2456144 |
| analysis of hpv-1 e4 gene expression using epitope-defined antibodies. | six monoclonal antibodies (mabs) have been raised against the e4 proteins of hpv-1. five of these were found to recognize denaturation-resistant epitopes as determined by western blotting--and their binding sites were identified by determining their reactivity against a panel of bacterial e4--beta-galactosidase fusion proteins which contained progressive deletions at the c-terminal end of the e4 region. the five mabs were found to bind to four distinct sites. by using these epitope-defined mabs, ... | 1988 | 2456213 |
| selective suppression of human papillomavirus transcription in non-tumorigenic cells by 5-azacytidine. | the transcription of human papillomavirus type 18 (hpv 18) is selectively suppressed in non-tumorigenic hela x fibroblast or hela x keratinocyte cell hybrids by 5-azacytidine. in contrast, viral gene expression is not influenced by 5-azacytidine in both tumorigenic hybrid segregants and in the parental hela cells. the suppression mechanism seems to operate at the level of initiation of transcription since nuclear run-on experiments show the absence of elongated nascent viral rna, whereas the tra ... | 1988 | 2457495 |
| detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. | human papilloma virus (hpv) dna sequences have been detected in paraffin-embedded tissue using an enzymatic in vitro amplification technique known as the polymerase chain reaction. amplification of a hpv dna sequence before its detection with a cdna probe significantly increases the rapidity as well as the sensitivity of detection such that a single 5-10-micron thick paraffin-embedded tissue section can be analyzed within 24 h. the assay specifically detected hpv 16 or 18 without crossreactivity ... | 1988 | 2826637 |
| physical state and biological activity of human papillomavirus genomes in precancerous lesions of the female genital tract. | the dna of distinct human papillomaviruses (hpvs) is regularly detected in the majority of human cervical carcinomas. in contrast to benign hpv-induced genital lesions, where the viral genomes are exclusively present as episomes, in cervical carcinomas hpv type 16 (hpv16) dna was found to be integrated into the host dna. in order to determine the physical state and expression of hpv dna sequences at different stages of tumour development, we analysed a series of cervical lesions (mild, moderate ... | 1988 | 2826665 |
| colposcopy is superior to cytology for the detection of early genital human papillomavirus infection. | of 2232 women with no cytologic evidence of intraepithelial neoplasia, 250 (11.2%) were positive for human papillomavirus deoxyribonucleic acid (dna) by filter in situ hybridization. in 150 of those human papillomavirus-positive patients, an adequate colposcopic examination of the cervix was possible; human papillomavirus infection was diagnosed in 104 women (70%). cervical cytology showed evidence of human papillomavirus infection in only 23 patients (15%). the following colposcopic features we ... | 1988 | 2827083 |
| papillomavirus in the vapor of carbon dioxide laser-treated verrucae. | vapor produced by the carbon dioxide laser during the vaporization of papillomavirus-infected verrucae was analyzed for viral dna content. two models were used for evaluation: an in vitro cutaneous bovine fibropapilloma and an in vivo human verruca model. four bovine fibropapillomas were exposed to various laser parameters with power densities of 38,200 to 130 w/cm2 and energy fluences of 3820 to 130 j/cm2. the generated vapor was collected in a chamber in line with a vacuum system. hybridizatio ... | 1988 | 2828703 |
| cervical cytology in the auckland region. | a two week prospective study of the characteristics of women having cervical smears in the auckland region in 1985 is presented. european and maori women have about the same rate of smears while pacific islanders have a slightly lesser rate. many women are not having smears taken during pregnancy or at the postnatal examination. doctors initiate twice as many smears as their patients. more than half of the women had smears taken in less than the three year interval recommended for screening. fiv ... | 1988 | 2832794 |
| antibody to human papilloma virus in danish dentists. | the aim of this study was to investigate the seroprevalence of antibody to human papilloma virus (hpv) among danish dentists in order to determine whether this virus constitutes an occupational hazard for dentists. serum samples from 452 danish dentists were tested for antibody against a common capsid antigen to hpv by a complement fixation reaction. nine (2.0%) were seropositive. dentists' odds ratio for seropositivity was after adjustment for age 0.6 (90% confidence interval: 0.3-1.4) compared ... | 1988 | 2832934 |
| functional mapping of the human papillomavirus type 11 transcriptional enhancer and its interaction with the trans-acting e2 proteins. | the transcriptional enhancer sequences of the papillomaviruses are regulated by trans-acting factors encoded by the viral e2 open reading frame. we have performed detailed functional and physical analyses of the enhancer of the human papillomavirus type 11 (hpv-11). using the chloramphenicol acetyltransferase (cat) assay in transiently transfected monkey cv-1 cells, the enhancer region has been localized to a 270-bp tract immediately preceding the e6 open reading frame, and it consists of two fu ... | 1988 | 2833426 |
| detection of human papillomavirus dna sequences by in situ dna-dna hybridisation in cervical intraepithelial neoplasia and invasive carcinoma: a retrospective study. | human papillomavirus (hpv) infection of cervical intraepithelial neoplasia (cin) and invasive cervical carcinoma was investigated using in situ dna-dna hybridisation on histological sections of formalin fixed, paraffin embedded tissue to assess the technique's sensitivity and to assess retrospectively the association between hpv16 and invasive cervical carcinoma. hpv dna was detected in 16 of 33 biopsy specimens of cin. cells containing viral dna were more numerous than those positive for viral ... | 1988 | 2834420 |
| human papillomavirus and herpes simplex virus in vulvar squamous cell carcinoma in situ. | human papillomavirus deoxyribonucleic acid were detected in tissue specimens from 38 to 46 patients (83%) with squamous cell carcinoma in situ of the vulva. herpes simplex virus type 2--related antigen icsp 34/35 was demonstrated in 23 of the lesions (50%), and antibodies to herpes simplex virus types 1 and 2 were found in 74% and 65% of the serum samples tested, respectively. both human papillomavirus deoxyribonucleic acid and herpes simplex virus type 2 antigen were detected in 19 cases (41%). ... | 1988 | 2834955 |
| identification of immunoreactive antigens of human papillomavirus type 6b by using escherichia coli-expressed fusion proteins. | human papillomavirus (hpvs) infect the genital epithelium and are found in proliferative lesions ranging from benign condylomata to invasive carcinomas. the immunological response to these infections is poorly understood because of the lack of purified viral antigens. in this study, bacterially derived fusion proteins expressing segments of all the major open reading frames (orfs) of hpv type 6b (hpv-6b) have been used in western blot (immunoblot) assays to detect antibodies directed against hpv ... | 1988 | 2835513 |
| anal cancer in women. | we studied predisposing factors in 56 women with anal cancer, comparing them with 56 matched controls drawn from the population. a detailed pretested questionnaire was administered to each study subject in a structured interview and blood was drawn for detection of herpes simplex virus antibodies by radioimmunoassay. pathologic material from cases was obtained and evidence of human papilloma virus infection was sought. by univariate analyses we found associations between anal cancer and positive ... | 1988 | 2836255 |
| oral squamous papillomas: detection of hpv dna by in situ hybridization. | oral squamous papillomas were segregated from other papillary lesions on the basis of histopathologic features. twenty representative papillomas were evaluated for the presence of papillomavirus genus-specific antigen with the use of an immunoperoxidase technique. these same tumors were analyzed for human papillomavirus (hpv) types 2, 4, 6, and 11 with biotinylated full-length double-stranded dna probes by in situ hybridization. only one case exhibited papillomavirus antigen reactivity. alternat ... | 1988 | 2836773 |
| expression of the e2 open reading frame of papillomaviruses bpv1 and hpv6b in silkworm by a baculovirus vector. | to produce and characterize the nature of the e2 protein of papillomaviruses, we have developed a system to express the dna sequence containing an open reading frame (orf) as a fusion protein in cultured insect cells and silkworms. the dna fragments of the e2 orf predicted from the dna sequence of bovine papillomavirus type 1 and human papillomavirus type 6b were linked to the n-terminal part of polyhedrin gene of the baculovirus bombyx mori nuclear polyhedrosis virus (bmnpv) vector. hybrid prot ... | 1988 | 2837019 |
| comparison of the cytobrush and cotton swabs in sampling cervical cells for filter in situ hybridization detection of human papillomavirus types 16 and 18 dna. | the cytobrush was compared with the cotton swab for collecting samples used to detect human papillomavirus (hpv) types 16 and 18 dna by filter in situ hybridization. the study design entailed collecting two endocervical and one vaginal fornix sample from each of 200 women admitted to a colposcopy clinic. no difference was found in the hpv positivity rates in samples obtained with the two collection instruments. there was good agreement (91.5%) in the detection of viral dna between the first and ... | 1988 | 2837033 |
| detection of human papillomavirus type 16 in bowenoid papulosis and invasive carcinoma occurring in the same patient with a history of cervical carcinoma. | 1988 | 2837151 | |
| presence and expression of human papillomavirus types 16 and 18 dna sequences in cervical carcinomas. | 1988 | 2837171 | |
| human papillomavirus and cancer of the uterine cervix. | 1988 | 2837428 | |
| human papillomavirus dna in adenosquamous carcinoma and squamous cell carcinoma of the vulva. | the tissues from 16 cases of adenosquamous carcinoma (pseudoglandular squamous cell carcinoma or adenoacanthoma of the sweat glands of lever) and 26 cases of invasive squamous cell carcinoma of the vulva were studied for the presence of human papillomavirus (hpv) genomes using southern blot hybridization on fresh tissues. types 1, 2, 3, 4, 5, 6, 16, and 18 hpv dna probes and in situ hybridization were used on formalin-fixed paraffin sections using type 2, 6, 16, and 18 hpv dna probes. only one c ... | 1988 | 2837709 |
| correlation of cytologic, colposcopic, and histologic studies with immunohistochemical studies of human papillomavirus structural antigens in an unselected patient population. | a study was undertaken to determine the prevalence of human papillomavirus infection in the general population. the first phase evaluated cytologic and colposcopic diagnoses in 962 consecutive patients in a community practice. the second, detailed phase evaluated 110 consecutive patients making routine visits in the same practice. studies performed for each patient in phase two were cervical cytology, cervical cytologic immunohistochemistry for human papillomavirus, colposcopy with biopsy of all ... | 1988 | 2837901 |
| sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 dna in frozen tissue sections of squamous cell carcinomas of the cervix. | the sensitivity of human papilloma virus type 16 (hpv-16) dna detection by dna in situ hybridization using biotinylated probes (bio-dish) was estimated by performing this technique on snap-frozen tissue sections of 10 cervical squamous cell carcinomas containing increasing amounts of hpv-16 as determined by southern blot hybridization. a protocol using serial sections for bio-dish and dna extraction was used. the number of positively stained cells and the detection limit were strongly dependent ... | 1988 | 2837906 |
| solitary condylomatous papilloma of the bronchus. | two case reports of solitary bronchial papillomas are presented. the histologic features of the lesions are those of squamous papillomas exhibiting prominent condylomatous changes. immunoperoxidase stains revealed the presence of human papillomavirus antigens in the koilocytotic cells. ultrastructural studies demonstrated possible intranuclear viral-size particles. solitary condylomatous papillomas of the bronchus should be distinguished from solitary bronchial squamous papillomas that do not ha ... | 1988 | 2838006 |
| condylomata acuminata of the urinary bladder. natural history, viral typing, and dna content. | three patients with condylomata acuminata of the urinary bladder are reported. two of the patients were immunosuppressed, and one had longstanding extensive condylomata acuminata of the external genitalia and adjacent areas. all lesions recurred at least once and were difficult to treat. the diagnosis was confirmed by in situ hybridization on archival material with human papillomavirus (hpv) dna probes under stringent conditions. in two of the patients, probes for hpv types 6 and 11 were positiv ... | 1988 | 2830802 |
| sexual transmission of human papillomaviruses in heterosexual and male homosexual couples, studied by dna hybridisation. | the prevalence of human papillomavirus (hpv) 6, 11, 16, and 18 in 36 heterosexual couples and seven male homosexual couples with genital warts was investigated for evidence of sexual transmission of genital hpv. the prevalence of virus type and number of copies of viral genome equivalents/cell in the lesions were assessed, and the factors influencing transmission analysed. our results show that hpv 6 and, to a lesser extent, hpv 11 were the types most readily transmitted, and that transmission a ... | 1988 | 2831137 |
| isolation of a novel human papillomavirus (type 51) from a cervical condyloma. | we cloned the dna from a novel human papillomavirus (hpv) present in a cervical condyloma. when dna from this isolate was hybridized at high stringency with hpv types 1 through 50 (hpv-1 through hpv-50), it showed weak homology with hpv-6 and -16 and stronger homology with hpv-26. a detailed restriction endonuclease map was prepared which showed marked differences from the maps for other hpvs that have been isolated from the female genital tract. reassociation kinetic analysis revealed that hpv- ... | 1988 | 2831406 |
| human papillomavirus: implications for clinical medicine. | 1988 | 2831766 | |
| does human papillomavirus cause cervical cancer? the state of the epidemiological evidence. | the human papillomavirus has emerged over the past decade as the leading candidate to be the sexually transmitted aetiological factor in cervical cancer. although it appears that papillomavirus types 16 and 18 are associated with a higher risk of advanced cervical neoplasia, most of the evidence comes from studies which do not satisfy basic epidemiological requirements, and are therefore difficult to interpret. the most significant problems are the small sample size, potentially biased selection ... | 1988 | 2831924 |
| prevalence of human papillomavirus types in the cervices of women before and after laser ablation. | 1988 | 2831935 | |
| viral infection, human papillomavirus dna, and cervical neoplasia. | 1988 | 2832035 | |
| pregnancy, human papillomavirus and cervical intraepithelial neoplasia. | human papillomavirus (hpv) has been implicated in the development of cervical intraepithelial neoplasia (cin). to test the hypothesis that the known relationship between hpv and cin might be modified by the altered immune status of pregnancy the cytologic, colposcopic and histologic findings in 176 pregnant and 515 nonpregnant patients referred to a colposcopy clinic were prospectively compared. no difference between the two groups was found regarding the frequency with which cin and hpv were de ... | 1988 | 2838281 |
| papillomaviruses in lesions of the lower genital tract in israeli patients. | human papillomaviruses (hpvs) have been strongly associated with benign lesions of the genital tract (condylomata) and with genital cancer of the vulva and cervix. since the incidence of these lesions in israel is considered to be low, we have studied the presence of hpv 6, 11, 16 and 18 dnas in benign, premalignant and malignant tissue samples or gynecological swabs of the lower genital tract. hpv sequences were detected in 48 out of 66 condylomatous lesions (72%), 5/11 grades i-ii intraepithel ... | 1988 | 2838295 |
| epidermodysplasia verruciformis in a patient with hodgkin's disease: characterization of a new papillomavirus type and interferon treatment. | a new human papillomavirus (hpv) was discovered in disseminated, macular, pityriasis versicolor-like lesions on the skin of the neck, face, scalp, and pubic region of a 42-year-old male suffering from hodgkin's disease. histopathology revealed features characteristic of epidermodysplasia verruciformis (ev). in contrast to classical ev, the lesions were almost exclusively seen in previously irradiated and uv-exposed skin areas. papillomavirus capsid antigen was demonstrated with the genus-specifi ... | 1988 | 2838553 |
| analysis of the l1 gene product of human papillomavirus type 16 by expression in a vaccinia virus recombinant. | the l1 open reading frame of human papillomavirus type 16 (hpv16) has been expressed in vaccinia virus under the control of both the 7.5k early and late promoter, and the 4b major late promoter. antibodies to a beta-galactosidase fusion protein containing a c-terminal portion of the hpv16 l1 gene product were used to compare the levels of l1 expression in the two recombinants, and showed that greater levels of expression were obtained when the gene was placed under the control of the 4b late pro ... | 1988 | 2838573 |
| characterization of human papillomavirus type 6b dna isolated from an invasive squamous carcinoma of the vulva. | human papillomavirus type 6 (hpv 6) dna was detected in an episomal form in dna extracted from an invasive squamous carcinoma of the vulva. the viral dna, designated hpv6-t70, was molecularly cloned. restriction analysis of the hpv6-t70 genome revealed an insertion of approximately 35 bp in the 5' portion of the upstream regulatory region relative to the prototype hpv 6b genome, cloned from a benign genital wart (e.-m. de villiers, l. gissmann, and h. zur hausen, 1981, j. virol. 40, 932-935). ho ... | 1988 | 2838960 |
| human papilloma virus findings in the perimeter of vulvo-vaginal malignancies. | during recent years demonstration of human papilloma virus (hpv) in cancers of the lower female genital tract has led to the assumption, that these viruses may be associated with the pathogenesis of these tumors. this theory is particularly important for patients with intraepithelial lesions of the cervix, vagina and vulva, vulvar dystrophies and condylomata acuminata of the vulva and vagina, which are seen in the dysplasia clinic of our hospital. using the in situ hybridization technique hpv 6/ ... | 1988 | 2839343 |