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azole susceptibility in clinical and environmental isolates of aspergillus fumigatus from eastern hokkaido, japan.azole antifungals are used not only clinically for fungal infections but also used as agricultural fungicides. recently, azole-resistant aspergillus fumigatus containing a tandem repeat in the promoter region of cyp51a combined with amino acid substitution(s) appears in the environment in eurasia, especially in several european countries. although azole fungicides have been used in japan, especially in hokkaido, surveillance and characterization of a. fumigatus in hokkaido have not been reported ...201627050399
gliotoxin suppresses macrophage immune function by subverting phosphatidylinositol 3,4,5-trisphosphate homeostasis.aspergillus fumigatus, an opportunistic fungal pathogen, spreads in the environment by releasing numerous conidia that are capable of reaching the small alveolar airways of mammalian hosts. in otherwise healthy individuals, macrophages are responsible for rapidly phagocytosing and eliminating these conidia, effectively curbing their germination and consequent invasion of pulmonary tissue. however, under some circumstances, the fungus evades phagocyte-mediated immunity and persists in the respira ...201627048806
genome-wide expression profiling reveals s100b as biomarker for invasive aspergillosis.invasive aspergillosis (ia) is a devastating opportunistic infection and its treatment constitutes a considerable burden for the health care system. immunocompromised patients are at an increased risk for ia, which is mainly caused by the species aspergillus fumigatus. an early and reliable diagnosis is required to initiate the appropriate antifungal therapy. however, diagnostic sensitivity and accuracy still needs to be improved, which can be achieved at least partly by the definition of new bi ...201627047454
a new antibacterial lupane ester from the seeds of acokanthera oppositifolia lam.as a part of ongoing investigation of acokanthera oppositifolia (lam.) codd., four compounds were isolated from its seeds, a new compound; lup-20(29)-en-3β-o-(3'-β-hydroxy) palmitate (1), three known compounds; a triterpene; lupeol (2), a cardiac glycoside; acovenoside a (3) and a sterol; β-sitosterol (4). their structures were investigated using 1d & 2d- (1)h and (13)cnmr spectroscopy. antimicrobial potential of the compounds was evaluated against 10 microorganisms responsible for endocarditis. ...201627046822
characterization of thermophilic fungal community associated with pile fermentation of pu-erh tea.this study aimed to characterize the thermophilic fungi in pile-fermentation process of pu-erh tea. physicochemical analyses showed that the high temperature and low ph provided optimal conditions for propagation of fungi. a number of fungi, including blastobotrys adeninivorans, thermomyces lanuginosus, rasamsonia emersonii, aspergillus fumigatus, rhizomucor pusillus, rasamsonia byssochlamydoides, rasamsonia cylindrospora, aspergillus tubingensis, aspergillus niger, candida tropicalis and fusari ...201627046629
fungal sensitisation in severe asthma is associated with the identification of aspergillus fumigatus in sputum.fungal sensitisation is an important factor in severe asthma, not only for allergic bronchopulmonary aspergillosis (abpa) but also the more recently described severe asthma with fungal sensitisation (safs). it is not known whether these diseases are driven by the presence of airway fungal colonisation. we aimed to determine if both safs and abpa were associated with airway isolation of aspergillus fumigatus and whether the frequency of isolation changed following anti-fungal treatment.201627043956
the role of autophagy-related proteins in candida albicans infections.autophagy plays an important role in maintaining cell homeostasis by providing nutrients during periods of starvation and removing damaged organelles from the cytoplasm. a marker in the autophagic process is the reversible conjugation of lc3, a membrane scaffolding protein, to double membrane autophagosomes. recently, a role for lc3 in the elimination of pathogenic bacteria and fungi, including candida albicans (c. albicans), was demonstrated, but these organisms reside in single membrane phagos ...201627043636
rice-field drowning-associated pneumonia in which pseudomonas spp., aspergillus fumigatus, and cunninghamella sp. are isolated.we herein report the case of an 84-year-old who developed pneumonia after drowning in a rice field. besides aspergillus fumigatus, many pathogens previously not reported in drowning-associated pneumonia (such as pseudomonas fluorescens, pseudomonas putida, nocardia niigatensis, and cunninghamella sp.) were isolated from his sputum. he received sulbactam/ampicillin, trimethoprim/sulfamethoxazole, voriconazole, levofloxacin and liposomal amphotericin b, but died due to respiratory failure. because ...201627041173
structural divergence of the group i intron binding surface in fungal mitochondrial tyrosyl-trna synthetases that function in rna splicing.the mitochondrial tyrosyl-trna synthetases (mttyrrss) of pezizomycotina fungi, a subphylum that includes many pathogenic species, are bifunctional proteins that both charge mitochondrial trna(tyr) and act as splicing cofactors for autocatalytic group i introns. previous studies showed that one of these proteins, neurospora crassa cyt-18, binds group i introns by using both its n-terminal catalytic and c-terminal anticodon binding domains and that the catalytic domain uses a newly evolved group i ...201627036943
a comparative study of the timecourse of the expression of the thermo‑inducible hsp70 gene in clinical and environmental isolates of aspergillus fumigatus.the internal environment within animals or humans provides different conditions to invading saprophytic fungal pathogens, requiring the differential regulation of genes in comparison to environmental conditions. understanding the mechanisms by which pathogens regulate genes within the host may be key in determining pathogen behavior within the host and may additionally facilitate further investigation into novel therapeutic agents. the heat shock protein (hsp)70 gene and its associated proteins ...201627035559
from synthetic dna to pcr product: detection of fungal infections using sers.we report the use of silver hydroxylamine nanoparticles functionalised with single stranded monothiolated dna for the detection of fungal infections. the four different species of fungi that were targeted were candida albicans, candida glabrata, candida krusei and aspergillus fumigatus. rational design of synthetic targets and probes was carried out by carefully analysing the 2-d folding of the dna and then by global alignment of the sequences to ensure specificity. the effects of varying the co ...201627034997
jak/stat regulation of aspergillus fumigatus corneal infections and il-6/23-stimulated neutrophil, il-17, elastase, and mmp9 activity.il-6 and il-23 (il-6/23) induce il-17a (il-17) production by a subpopulation of murine and human neutrophils, resulting in autocrine il-17 activation, enhanced production of reactive oxygen species, and increased fungal killing. as il-6 and il-23 receptors trigger jak1, -3/stat3 and jak2/stat3 phosphorylation, respectively, we examined the role of this pathway in a murine model of fungal keratitis and also examined neutrophil elastase and gelatinase (matrix metalloproteinase 9) activity by il-6/ ...201627034404
culturable fungi in potting soils and compost.in the present study the spectrum and the incidence of fungi in potting soils and compost was investigated. since soil is one of the most important biotopes for fungi, relatively high concentrations of fungal propagules are to be expected. for detection of fungi, samples of commercial soils, compost and soils from potted plants (both surface and sub-surface) were suspended and plated onto several mycological media. the resulting colonies were evaluated qualitatively and quantitatively. the resul ...201627354488
microbial biofilms in pulmonary and critical care diseases.microbial biofilms can colonize medical devices and human tissues, and their role in microbial pathogenesis is now well established. not only are biofilms ubiquitous in natural and human-made environments, but they are also estimated to be associated with approximately two-thirds of nosocomial infections. this multicellular aggregated form of microbial growth confers a remarkable resistance to killing by antimicrobials and host defenses, leading biofilms to cause a wide range of subacute or chro ...201627348071
important factors mediates the adhesion of aspergillus fumigatus to alveolar epithelial cells with e-cadherin.aspergillus is widely distributed in the earth's biosphere. it has strong adaptive capacity, and lives as saprophytic or parasitic life. this study aims to investigate the role of e-cadherin for adhesion of aspergillus fumigatus blastospores in a human epithelial cell line (a549) and search the correlated molecule in aspergillus. a. fumigatus blastospores were incubated with the total protein of a549 to investigate the binding of e-cadherin and blastospores followed by an affinity purification p ...201627347350
krüppel-like factor 4 modulates interleukin-6 release in human dendritic cells after in vitro stimulation with aspergillus fumigatus and candida albicans.invasive fungal infections are associated with high mortality rates and are mostly caused by the opportunistic fungi aspergillus fumigatus and candida albicans. immune responses against these fungi are still not fully understood. dendritic cells (dcs) are crucial players in initiating innate and adaptive immune responses against fungal infections. the immunomodulatory effects of fungi were compared to the bacterial stimulus lps to determine key players in the immune response to fungal infections ...201627346433
correction for de heer et al., detection of airway colonization by aspergillus fumigatus by use of electronic nose technology in patients with cystic fibrosis. 201627343292
contribution of molecular tools for the diagnosis and epidemiology of fungal chronic rhinosinusitis.chronic rhinosinusitis (crs) rank second at chronic inflammatory diseases in industrialized countries and are an important public health concern. diagnosis relies on a set of arguments including clinical signs, imaging, histopathologic and mycological analyses of sinus specimens, collected during nasal endoscopy. the sensitivity of fungal cultures is reported to be poor, even when direct examination is positive, thus the epidemiology of fungal chronic sinusitis is ill-known. this study evaluated ...201627335058
high-throughput sequencing reveals unprecedented diversities of aspergillus species in outdoor air.this study used the illumina miseq to analyse compositions and diversities of aspergillus species in outdoor air. the seasonal air samplings were performed at two locations in seoul, south korea. the results showed the relative abundances of all aspergillus species combined ranging from 0·20 to 18% and from 0·19 to 21% based on the number of the internal transcribed spacer 1 (its1) and β-tubulin (bena) gene sequences respectively. aspergillus fumigatus was the most dominant species with the mean ...201627333577
antifungal activity of plasmacytoid dendritic cells against cryptococcus neoformans in vitro requires expression of dectin-3 (clec4d) and reactive oxygen species.conventional dendritic cells (cdcs) are critical for protection against pulmonary infection with the opportunistic fungal pathogen cryptococcus neoformans; however, the role of plasmacytoid dendritic cells (pdcs) is unknown. we show for the first time that murine pdcs have direct activity against c. neoformans via reactive oxygen species (ros), a mechanism different from that employed to control aspergillus fumigatus infections. the anticryptococcal activity of murine pdcs is independent of opso ...201627324480
treatment with rhdnase in patients with cystic fibrosis alters in-vitro chit-1 activity of isolated leucocytes.recent data suggest a possible relationship between cystic fibrosis (cf) pharmacotherapy, aspergillus fumigatus colonization (ac) and/or allergic bronchopulmonary aspergillosis (abpa). the aim of this study was to determine if anti-fungal defence mechanisms are influenced by cf pharmacotherapy, i.e. if (1) neutrophils form cf and non-cf donors differ in their ability to produce chitotriosidase (chit-1); (2) if incubation of isolated neutrophils with azithromycin, salbutamol, prednisolone or rhdn ...201627324468
proteomic analysis of aspergillus fumigatus - clinical implications.aspergillus fumigatus is a ubiquitous saprophytic fungus capable of producing small airborne spores, which are frequently inhaled by humans. in healthy individuals, the fungus is rapidly cleared by innate mechanisms, including immune cells. however, in individuals with impaired lung function or immunosuppression the spores can germinate and prompt severe allergic responses, and disease with limited or extensive invasiveness.201627324303
synthesis and structure-activity relationship of some new thiophene-based heterocycles as potential antimicrobial agents.several new pyrazole, pyridine, [1,2,4]triazolo[1,5-α]pyrimidine, benzimidazo[1,2-a]pyrimidine and 1,2,4-triazolo[3,4-c][1,2,4]triazine derivatives incorporating a thiophene moiety were synthesized from (e)-ethyl 5-(3-(dimethylamino)acryloyl)-4-phenyl-2-(phenylamino)thiophene-3-carboxylate (1). the structures of the newly synthesized compounds were confirmed by ir, ¹h-, (13)c-nmr, mass spectral data and elemental analysis. the antibacterial and antifungal activities of all the synthesized compou ...201627517888
sensitization to food and inhalant allergens in healthy children in van, east turkey.the purpose of this study was to determine the most frequent food and inhalant allergens leading to allergic sensitization in children in van province of turkey.201627511484
comparative performance evaluation of multi-metal resistant fungal strains for simultaneous removal of multiple hazardous metals.in the present study, five fungal strains viz., aspergillus terreus aml02, paecilomyces fumosoroseus 4099, beauveria bassiana 4580, aspergillus terreus pd-17, aspergillus fumigatus pd-18, were screened for simultaneous multimetal removal. highest metal tolerance index for each individual metal viz., cd, cr, cu, ni, pb and zn (500mg/l) was recorded for a. fumigatus for the metals (cd, 0.72; cu, 0.72; pb, 1.02; zn, 0.94) followed by b. bassiana for the metals (cd, 0.56; cu, 0.14; ni, 0.29; zn, 0.8 ...201627497228
dose optimization of voriconazole/anidulafungin combination against aspergillus fumigatus using an in vitro pharmacokinetic/pharmacodynamic model and response surface analysis: clinical implications for azole-resistant aspergillosis.combination therapy of voriconazole with an echinocandin is often employed in order to increase the efficacy of voriconazole monotherapy.201627494912
azole resistance surveillance in aspergillus fumigatus: beneficial or biased?azole resistance is a growing concern with aspergillus fumigatus, and may cause increased mortality in patients with azole-resistant invasive aspergillosis (ia). microbial surveillance has been recognized as a fundamental component of resistance management. surveillance information may be used to inform decisions regarding health services and research funding allocation, to guide local infection control in hospitals and communities, and to direct local and national drug policies and guidelines. ...201627494831
new therapeutic strategies for invasive aspergillosis in the era of azole resistance: how should the prevalence of azole resistance be defined?given reports showing a high prevalence of azole resistance in aspergillus fumigatus, alternatives to azole therapy are discussed when a threshold of 10% of azole-resistant environmental isolates is reached. this raises the issue of calculation of this threshold, either on the prevalence of azole-resistant isolates as a whole or on the prevalence of azole-resistant cases in populations at risk of invasive aspergillosis (ia). for isolate evaluation, there are high disparities in routine microbiol ...201627494830
characterization of the trna ligases of pathogenic fungi aspergillus fumigatus and coccidioides immitis.yeast trna ligase (trl1) is an essential trifunctional enzyme that repairs rna breaks with 2',3'-cyclic-po4 and 5'-oh ends. trl1 is composed of c-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to generate the 3'-oh, 2'-po4, and 5'-po4 termini required for sealing by an n-terminal ligase domain. trl1 enzymes are found in all human fungal pathogens and they are promising targets for antifungal drug discovery because: (i) their domain structure ...201627492257
application of uv-vis spectrophotometric process for the assessment of indoloacridines as free radical scavenger.a conventional approach has been used to synthesis indole fused acridine, 4a-e. in this paper to achieve the target molecule, 4 the reaction was performed via two steps. in step 1, there was a reaction between carbazolone, 1 and benzophenone, 2 to get dihydroindoloacridine, 3. in step 2, compound, 3 was treated with 5% palladium/carbon in the presence of diphenyl ether for 5h to give a dark brown product, 4. the column chromatography was used to purify final product, 4. all the synthesized compo ...201627491030
aspergillus fumigatus components distinguish ige but not igg4 profiles between fungal sensitization and allergic broncho-pulmonary aspergillosis.aspergillus fumigatus is the causative agent of allergic broncho-pulmonary aspergillosis. prompt and accurate diagnosis may be difficult to achieve with current clinical and laboratory scores, which do not include immune responses to recombinant a. fumigatus allergens. we measured specific immunoglobulin e and g4 directed to recombinant a. fumigatus allergens in 55 cystic fibrosis patients without allergic broncho-pulmonary aspergillosis but sensitized to a. fumigatus and in nine patients with a ...201627542151
emerging aspergillosis by azole-resistant aspergillus fumigatus at an intensive care unit in the netherlands, 2010 to 2013.the prevalence of invasive aspergillosis (ia) at the intensive care unit (icu) is unknown and difficult to assess since ia also develops in patients lacking specific host factors. in the netherlands, increasing azole-resistance in aspergillus fumigatus complicates treatment of patients with ia. the aim of this study was to determine the prevalence of ia by azole-resistant a. fumigatus at the icu among patients receiving antifungal treatment and to follow their clinical outcome and prognosis. a r ...201627541498
der f 34, a novel major house dust mite allergen belonging to a highly conserved rid/yjgf/yer057c/uk114 family of imine deaminases.the high prevalence of house dust mite (hdm) allergy is a growing health problem worldwide, and the characterization of clinically important hdm allergens is a prerequisite for the development of diagnostic and therapeutic strategies. here, we report a novel hdm allergen that belongs structurally to the highly conserved rid/yjgf/yer057c/uk114 family (rid family) with imine deaminase activity. isolated hdm cdna, named der f 34, encodes 128 amino acids homologous to rid-like proteins. this new pro ...201627539850
the aspergillus fumigatus scha(sch9) kinase modulates saka(hog1) map kinase activity and it is essential for virulence.the serine-threonine kinase tor, the target of rapamycin, is an important regulator of nutrient, energy and stress signaling in eukaryotes. sch9, a ser/thr kinase of agc family (the camp-dependent pka, cgmp- dependent protein kinase g and phospholipid-dependent protein kinase c family), is a substrate of tor. here, we characterized the fungal opportunistic pathogen aspergillus fumigatus sch9 homologue (scha). the scha null mutant was sensitive to rapamycin, high concentrations of calcium, hypero ...201627538790
pcr-based detection of aspergillus fumigatus cyp51a mutations on bronchoalveolar lavage: a multicentre validation of the aspergenius assay® in 201 patients with haematological disease suspected for invasive aspergillosis.in patients with invasive aspergillosis (ia), fungal cultures are mostly negative. consequently, azole resistance often remains undetected. the aspergenius(®) multiplex real-time pcr assay identifies clinically relevant aspergillus species and four resistance-associated mutations (rams; tr34/l98h/t289a/y121f) in the cyp51a gene. this multicentre study evaluated the diagnostic performance of this assay on bronchoalveolar lavage (bal) fluid and correlated the presence of rams with azole treatment ...201627530755
direct molecular diagnosis of aspergillosis and cyp51a profiling from respiratory samples of french patients.microbiological diagnosis of aspergillosis and triazole resistance is limited by poor culture yield. to better estimate this shortcoming, we compared culture and molecular detection of a. fumigatus in respiratory samples from french patients at risk for aspergillosis.201627524978
preparation, characterization and antimicrobial applications of zn-fe ldh against mrsa.facile and simple processes to get zn-fe layered double hydroxide (ldh) with nitrate as the interlayer anion are reported. the method of co-precipitation produced high crystallinity ldh that is marked by xrd, sem, tem and ft-ir. results showed that 99.8% of cd(+2) removals were at ph11 and 4h. to get the adsorption isotherms, the concentration of metal ions extending from 6 to 18mg/l was utilized. results supported the langmuir adsorption model. in contrary, the adsorption process followed the p ...201627524011
demethylase inhibitor fungicide resistance in pyrenophora teres f. sp. teres associated with target site modification and inducible overexpression of cyp51.pyrenophora teres f. sp. teres is the cause of net form of net blotch (nfnb), an economically important foliar disease in barley (hordeum vulgare). net and spot forms of net blotch are widely controlled using site-specific systemic fungicides. although resistance to succinate dehydrogenase inhibitors and quinone outside inhibitors has been addressed before in net blotches, mechanisms controlling demethylation inhibitor resistance have not yet been reported at the molecular level. here we report ...201627594852
aspergillus fumigatus carrying tr34/l98h resistance allele causing complicated suppurative otitis media in tanzania: call for improved diagnosis of fungi in sub-saharan africa.suppurative otitis media (som) is a major public health concern worldwide and is associated with increased morbidity. cases of fungal suppurative otitis media were studied to establish the effect of fungi in otitis media.201627589956
inhibition of the flavin-dependent monooxygenase siderophore a (sida) blocks siderophore biosynthesis and aspergillus fumigatus growth.aspergillus fumigatus is an opportunistic fungal pathogen and the most common causative agent of fatal invasive mycoses. the flavin-dependent monooxygenase siderophore a (sida) catalyzes the oxygen and nadph dependent hydroxylation of l-ornithine (l-orn) to n(5)-l-hydroxyornithine in the biosynthetic pathway of hydroxamate-containing siderophores in a. fumigatus. deletion of the gene that codes for sida has shown that it is essential in establishing infection in mice models. here, a fluorescence ...201627588426
characterization of gprk encoding a putative hybrid g-protein-coupled receptor in aspergillus fumigatus.the g-protein-coupled receptor (gpcr) family represents the largest and most varied collection of membrane embedded proteins that are sensitized by ligand binding and interact with heterotrimeric g proteins. despite their presumed critical roles in fungal biology, the functions of the gpcr family members in the opportunistic human pathogen aspergillus fumigatus are largely unknown, as only two (gprc and gprd) of the 15 predicted gpcrs have been studied. here, we characterize the gprk gene, which ...201627584150
the current status of drug-resistant aspergillus.aspergillus spp. are important fungi because of the high mortality associated with aspergillosis. however, we have only three classes of anti-aspergillus drugs available; and only two drugs, itraconazole (itcz) and voriconazole (vrcz), are available as oral agents in japan. if azole-resistant aspergillus causes chronic aspergillosis, we cannot treat such patients with oral azoles on an outpatient basis. as is the case with foreign countries, we found the existence of azole-resistant aspergillus ...201627581778
cyclophilin and enolase are the most prevalent conidial antigens of lomentospora prolificans recognized by healthy human salivary iga and cross-react with aspergillus fumigatus.the study of the immunocompetent airways immune response may provide important information to improve the therapeutic efficacy against lomentospora (scedosporium) prolificans. so, this study aimed to identify the most prevalent conidial antigens of this multiresistant fungus recognized by healthy human salivary immunoglobulin a, and to study their expression and cross-reactivity with other fungal species.201627485921
sudden death of a siamese crocodile (crocodylus siamensis) due to systemic aspergillosis.a 4-year-old female siamese crocodile (crocodylus siamensis) housed at a zoo died without any prior clinical signs. during necropsy, numerous scattered, well-demarcated, yellowish-white, firm nodules were observed throughout the liver and lungs. microscopic examination with periodic acid-schiff staining revealed granulomatous inflammation in the liver and lungs. liver granulomas were characterized by the presence of a connective tissue barrier and hyphae, and the centers of the granulomas showed ...201627476559
th2 and th9 responses in patients with chronic mucocutaneous candidiasis and hyper-ige syndrome.stat1 mutations cause chronic mucocutaneous candidiasis (cmc), while stat3 mutations cause hyper-ige syndrome (hies). cmc and hies patients have t helper (th) 17 defects suffering from mucosal candida infections, but only patients with hies show an allergic phenotype with eczema, eosinophilia and high ige levels.201627474157
influence of aspergillus fumigatus conidia viability on murine pulmonary microrna and mrna expression following subchronic inhalation exposure.personal exposure to fungal bioaerosols derived from contaminated building materials or agricultural commodities may induce or exacerbate a variety of adverse health effects. the genomic mechanisms that underlie pulmonary immune responses to fungal bioaerosols have remained unclear.201627473664
aspergillus fumigatus mads-box transcription factor rlma is required for regulation of the cell wall integrity and virulence.the cell wall integrity (cwi) pathway is the primary signaling cascade that controls the de novo synthesis of the fungal cell wall, and in saccharomyces cerevisiae this event is highly dependent on the rlm1 transcription factor. here, we investigated the function of rlma in the fungal pathogen aspergillus fumigatus we show that the δrlma strain exhibits an altered cell wall organization in addition to defects related to vegetative growth and tolerance to cell wall-perturbing agents. a genetic an ...201627473315
pf4 bacteriophage produced by pseudomonas aeruginosa inhibits aspergillus fumigatus metabolism via iron sequestration.pseudomonas aeruginosa (pa) and aspergillus fumigatus (af) are major human pathogens known to interact in a variety of disease settings, including airway infections in cystic fibrosis. we recently reported that clinical cf isolates of pa inhibit the formation and growth of af biofilms. here, we report that the bacteriophage pf4, produced by pa, can inhibit the metabolic activity of af biofilms. this phage-mediated inhibition was dose dependent, ablated by phage denaturation, and was more pronoun ...201627473221
aspergillus fumigatus spore proteomics and genetics reveal that vea represses defa-mediated dna damage response.aspergillus fumigatus reproduces and infects host by forming a high number of small asexual spores (conidia). the velvet proteins are global transcriptional regulators governing the complex process of conidiogenesis in this fungus. here, to further understand the velvet-mediated regulation, we carried out comparative proteomic analyses of conidia of wild type (wt) and three velvet mutants (δvea, δvelb and δvosa). cluster analysis of 184 protein spots showing at least 1.5-fold differential accumu ...201627469890
dynamic immune cell recruitment after murine pulmonary aspergillus fumigatus infection under different immunosuppressive regimens.humans are continuously exposed to airborne spores of the saprophytic fungus aspergillus fumigatus. however, in healthy individuals pulmonary host defense mechanisms efficiently eliminate the fungus. in contrast, a. fumigatus causes devastating infections in immunocompromised patients. host immune responses against a. fumigatus lung infections in immunocompromised conditions have remained largely elusive. given the dynamic changes in immune cell subsets within tissues upon immunosuppressive ther ...201627468286
udp-galactopyranose mutase, a potential drug target against human pathogenic nematode brugia malayi.lymphatic filariasis, a vector-borne neglected tropical disease affects millions of population in tropical and subtropical countries. vaccine unavailability and emerging drug resistance against standard antifilarial drugs necessitate search of novel drug targets for developing alternate drugs. recently, udp-galactopyranose mutases (ugm) have emerged as a promising drug target playing an important role in parasite virulence and survival. this study deals with the cloning and characterization of b ...201627465638
biogenic synthesis, optimisation and antibacterial efficacy of extracellular silver nanoparticles using novel fungal isolate aspergillus fumigatus ma.to eliminate the elaborate processes employed in other non-biological-based protocols and low cost production of silver nanoparticles (agnps), this study reports biogenic synthesis of agnps using silver salt precursor with aqueous extract of aspergillus fumigates ma. influence of silver precursor concentrations, concentration ratio of fungal extract and silver nitrate, contact time, reaction temperature and ph are evaluated to find their effects on agnps synthesis. ultraviolet-visible spectra ga ...201627463792
correction for escribano et al., microsatellite (straf) genotyping cannot differentiate between invasive and colonizing aspergillus fumigatus isolates. 201627458273
use of scw4 gene primers in pcr methods for the identification of six medically important aspergillus species.aspergillus species are the cause of invasive mold infections in immunocompromised patients: aspergillus fumigatus, a. flavus and a. terreus account for most cases of invasive aspergillosis (ia). as certain species are associated with higher mortality and vary in their resistance to antifungal therapy, diagnosis requires increasingly rapid molecular methods that enable sensitive detection and species discrimination. we have developed pcr and multiplex pcr assays for the detection of six medicall ...201627455180
new cyclotetrapeptides and a new diketopiperzine derivative from the marine sponge-associated fungus neosartorya glabra kufa 0702.two new cyclotetrapeptides, sartoryglabramides a (5) and b (6), and a new analog of fellutanine a (8) were isolated, together with six known compounds including ergosta-4, 6, 8 (14), 22-tetraen-3-one, ergosterol 5, 8-endoperoxide, helvolic acid, aszonalenin (1), (3r)-3-(1h-indol-3-ylmethyl)-3,4-dihydro-1h-1,4-benzodiazepine-2,5-dione (2), takakiamide (3), (11ar)-2,3-dihydro-1h-pyrrolo[2,1-c][1,4]benzodiazepine-5,11(10h,11ah)-dione (4), and fellutanine a (7), from the ethyl acetate extract of the ...201627447650
enose technology can detect and classify human pathogenic molds in vitro: a proof-of-concept study of aspergillus fumigatus and rhizopus oryzae.invasive pulmonary mold disease (ipmd) is often fatal in neutropenic patients. this is because ipmd is difficult to diagnose timely, especially when non-aspergillus molds are the causative agent, as they are usually not associated with a positive galactomannan assay. in 2013 we showed that exhaled breath analysis might be used to diagnose invasive aspergillosis through profiling of patterns in exhaled volatile organic compounds (vocs) by electronic nose (enose) technology. the current study aime ...201627447026
dephosphorylation of the core septin, aspb, in a protein phosphatase 2a-dependent manner impacts its localization and function in the fungal pathogen aspergillus fumigatus.septins are a conserved family of gtpases that form hetero-oligomeric complexes and perform diverse functions in higher eukaryotes, excluding plants. our previous studies in the human fungal pathogen aspergillus fumigatus revealed that the core septin, aspb, a cdc3 ortholog, is required for septation, conidiation, and conidial cell wall organization. although aspb is important for these cellular functions, nothing is known about the role of kinases or phosphatases in the posttranslational regula ...201627446037
invasive pulmonary aspergillosis with disseminated infection in immunocompetent patient.invasive pulmonary aspergillosis (ipa) is a rare pathology with increasing incidence mainly in critical care settings and recently in immunocompetent patients. the mortality of the disease is very high, regardless of an early diagnosis and aggressive treatment. here, we report a case of a 56 yr old previously healthy woman who was found unconscious at home and admitted to the emergency room with mild respiratory insufficiency. in the first 24 hours she developed an acute respiratory failure with ...201627445566
an iterative o-methyltransferase catalyzes 1,11-dimethylation of aspergillus fumigatus fumaric acid amides.s-adenosyl-l-methionine (sam)-dependent methyltransfer is a common biosynthetic strategy to modify natural products. we investigated the previously uncharacterized aspergillus fumigatus methyltransferase ftpm, which is encoded next to the bimodular fumaric acid amide synthetase ftpa. structure elucidation of two new a. fumigatus natural products, the 1,11-dimethyl esters of fumaryl-l-tyrosine and fumaryl-l-phenylalanine, together with ftpm gene disruption suggested that ftpm catalyzes iterative ...201627442960
new polyketides and new benzoic acid derivatives from the marine sponge-associated fungus neosartorya quadricincta kufa 0081.two new pentaketides, including a new benzofuran-1-one derivative (1) and a new isochromen-1-one (5), and seven new benzoic acid derivatives, including two new benzopyran derivatives (2a, b), a new benzoxepine derivative (3), two new chromen-4-one derivatives (4b, 7) and two new benzofuran derivatives (6a, b), were isolated, together with the previously reported 2,3-dihydro-6-hydroxy-2,2-dimethyl-4h-1-benzopyran-4-one (4a), from the culture of the marine sponge-associated fungus neosartorya quad ...201627438842
sterol biosynthesis and azole tolerance is governed by the opposing actions of srba and the ccaat binding complex.azole drugs selectively target fungal sterol biosynthesis and are critical to our antifungal therapeutic arsenal. however, resistance to this class of drugs, particularly in the major human mould pathogen aspergillus fumigatus, is emerging and reaching levels that have prompted some to suggest that there is a realistic probability that they will be lost for clinical use. the dominating class of pan-azole resistant isolates is characterized by the presence of a tandem repeat of at least 34 bases ...201627438727
mitochondrial complex i is a global regulator of secondary metabolism, virulence and azole sensitivity in fungi.recent estimates of the global burden of fungal disease suggest that that their incidence has been drastically underestimated and that mortality may rival that of malaria or tuberculosis. azoles are the principal class of antifungal drug and the only available oral treatment for fungal disease. recent occurrence and increase in azole resistance is a major concern worldwide. known azole resistance mechanisms include over-expression of efflux pumps and mutation of the gene encoding the target prot ...201627438017
a putative mitochondrial iron transporter mrsa in aspergillus fumigatus plays important roles in azole-, oxidative stress responses and virulence.iron is an essential nutrient and enzyme co-factor required for a wide range of cellular processes, especially for the function of mitochondria. for the opportunistic fungal pathogen aspergillus fumigatus, the ability to obtain iron is required for growth and virulence during the infection process. however, knowledge of how mitochondria are involved in iron regulation is still limited. here, we show that a mitochondrial iron transporter, mrsa, a homolog of yeast mrs4p, is critical for adaptation ...201627433157
epidemiology and molecular characterizations of azole resistance in clinical and environmental aspergillus fumigatus isolates from china.azole resistance in aspergillus fumigatus has emerged as a worldwide public health problem. we sought here to demonstrate the occurrence and characteristics of azole resistance in a. fumigatus from different parts of china. a total of 317 clinical and 144 environmental a. fumigatus isolates from 12 provinces were collected and subjected to screening for azole resistance. antifungal susceptibility, cyp51a gene sequencing, and genotyping were carried out for all suspected azole-resistant isolates ...201627431231
the evolution of water extractable organic matter and its association with microbial community dynamics during municipal solid waste composting.the humification of water extractable organic matter (weom) by microorganisms is widely used for assessing compost maturity and quality. however, the effect of bacterial and fungal community dynamics on humification of weom was not yet explored fully. here, we used canonical correspondence analysis (cca) and redundancy analysis (rda) to investigate the link between bacterial and fungal community dynamics and humification process of weom, respectively. results showed that water-soluble carbon (ws ...201627425858
human immunodeficiency virus and allergic bronchopulmonary aspergillosis: case report and review of literature.allergic bronchopulmonary aspergillosis (abpa) results from a hypersensitivity response to airways colonization with aspergillus fumigatus, and it occurs most often in individuals with asthma or cystic fibrosis. allergic bronchopulmonary aspergillosis is an indolent, but potentially progressive, disease in patients. in patients infected with human immunodeficiency virus (hiv), abpa is rare, and its description in the literature is limited to case reports. we describe the occurrence of abpa in a ...201627419184
draft genome sequences of two aspergillus fumigatus strains, isolated from the international space station.draft genome sequences of aspergillus fumigatus strains (issft-021 and if1sw-f4), opportunistic pathogens isolated from the international space station (iss), were assembled to facilitate investigations of the nature of the virulence characteristics of the iss strains to other clinical strains isolated on earth.201627417828
assessment of relevant fungal species in clinical solid wastes.the study aimed to determine the fungal diversity in clinical waste samples from a healthcare facility in penang malaysia. different fungi species were detected in 83.75 % of the 92 clinical waste samples that were screened from different sections of the healthcare facility. one hundred fifty fungal isolates comprising of 8 genera and 36 species were obtained. they were purified by using single spore isolation technique. subsequently, the isolates were identified by phenotypic method based on mo ...201627417327
a sweet response to a sour situation: the role of soluble pattern recognition receptors in the innate immune response to invasive aspergillus fumigatus infections. 201627415780
mtor modulates lymphocyte differentiation through t-bet and eomesodermin in response to invasive pulmonary aspergillosis in rats.aspergillosis infection is common in the patients with insufficient immunity. the role of mammalian target of rapamycin (mtor), t-box expressed in t-cells (t-bet), and eomesodermin (eomes) in mediating t lymphocytes differentiation in response to aspergillus fumigatus infection in immunocompromised rats was investigated in this study.201627411458
corrigendum to "first detection of aspergillus fumigatus azole resistant strain due to cyp51a tr46/y121f/t289a in an azole-naive patient in spain" [new microbes new infect 6 (2015) 33-34].[this corrects the article doi: 10.1016/j.nmni.2015.04.005.].201627408747
administration of zinc chelators improves survival of mice infected with aspergillus fumigatus both in monotherapy and in combination with caspofungin.aspergillus fumigatus can infect immunocompromised patients, leading to high mortality rates due to the lack of reliable treatment options. this pathogen requires uptake of zinc from host tissues in order to successfully grow and cause virulence. reducing the availability of that micronutrient could help treat a. fumigatus infections. in this study, we examined the in vitro effects of seven chelators using a bioluminescent strain of a. fumigatus 1,10-phenanthroline and n,n,n',n'-tetrakis(2-pyrid ...201627401578
diagnosis of invasive pulmonary aspergillosis.culturing strains from clinical samples is the main method to diagnose invasive pulmonary aspergillosis. detecting the galactomannan antigen in serum samples is an auxiliary examination. the goal of this study was to determine the frequency with which aspergillus fumigatus was cultured in clinical samples taken from patients hospitalized in the the infant jesus teaching hospital in warsaw, poland, in the period of 2013-2014. specimens from the respiratory tract and blood were cultured for mycolo ...201627401078
evaluation of exposure of pemphigus vulgaris patients to mycobacterium tuberculosis and aspergillus fumigatus.the purpose of this study was to screen pemphigus vulgaris (pv) (autoimmune bullous skin disease) for the presence of immunoglobulin g against mycobacterium tuberculosis and aspergillus fumigatus. the sera of 60 pv patients and 28 controls were screened for the presence of immunoglobulin g against m. tuberculosis and a. fumigatus by enzyme-linked immune-sorbent assay. forty patients were females and 20 were males. the range of igg against m. tuberculosis was from 0.9 to 152.6 (median = 2.95) in ...201627396270
the aspergillus fumigatus conidial melanin production is regulated by the bifunctional bhlh devr and mads-box rlma transcription factors.melanins play a crucial role in defending organisms against external stressors. in several pathogenic fungi, including the human pathogen aspergillus fumigatus, melanin production was shown to contribute to virulence. a. fumigatus produces two different types of melanins, i.e., pyomelanin and dihydroxynaphthalene (dhn)-melanin. dhn-melanin forms the gray-green pigment characteristic for conidia, playing an important role in immune evasion of conidia and thus for fungal virulence. the dhn-melanin ...201627393422
silver adducts of four-branched histidine rich peptides exhibit synergistic antifungal activity.previously, a four branched histidine-lysine rich peptide, h3k4b, was shown to demonstrate selective antifungal activity with minimal antibacterial activity. due to the potential breakdown from proteases, h3k4b was further evaluated in the current study by varying the d- and l-amino acid content in its branches. whereas analogues of h3k4b that selectively replaced l-amino acids (h3k4b, h3k4b) had improved antifungal activity, the all d-amino acid analogue, h3k4b, had reduced activity, suggesting ...201627387239
aspergillus fumigatus intrinsic fluconazole resistance is due to the naturally occurring t301i substitution in cyp51ap.aspergillus fumigatus intrinsic fluconazole resistance has been demonstrated to be linked to the cyp51a gene, although the precise molecular mechanism has not been elucidated yet. comparisons between a. fumigatus cyp51ap and candida albicans erg11p sequences showed differences in amino acid residues already associated with fluconazole resistance in c. albicans the aim of this study was to analyze the role of the natural polymorphism i301 in aspergillus fumigatus cyp51ap in the intrinsic fluconaz ...201627381395
aspergillus fumigatus endophthalmitis with necrotizing scleritis following pars plana vitrectomy.we present a case of aspergillus fumigatus endophthalmitis complicated by necrotizing scleritis in a 68-year-old man with diet-controlled diabetes, after retinal detachment repair. he was initially treated with systemic steroids for surgically induced necrotizing scleritis following routine pars plana vitrectomy. an additional diagnosis of endophthalmitis was made when the patient developed a hypopyon. repeat vitreous culture isolated aspergillus fumigatus. symptoms improved following antifungal ...201627379189
a putative mitochondrial calcium uniporter in a. fumigatus contributes to mitochondrial ca(2+) homeostasis and stress responses.ca(2+) uptake into mitochondria plays a central role in cell physiology by stimulating atp production, shaping cytosolic ca(2+) transients and regulating cell survival or death. although this system has been studied extensively in mammalian cells, the physiological implications of ca(2+) uptake into mitochondria in fungal cells are still unknown. in this study, a bi-directional best-hit blastp search revealed that the genome of aspergillus fumigatus encodes a homolog of a putative mitochondrial ...201627378202
concurrent sensitization to aspergillus fumigatus in tropical pulmonary eosinophilia.tropical pulmonary eosinophilia (tpe) is characterized by lung tissue and peripheral blood eosinophilia. serum total ige is also markedly increased in tpe. however, an association with asthma or other hypersensitivity conditions has not been described. during the diagnostic workup of three patients eventually confirmed to have tpe, hypersensitivity to the fungus, aspergillus fumigatus was found. however, there was no evidence of diseases of aspergillus hypersensitivity such as severe asthma with ...201627374215
improved extracellular expression and high-cell-density fed-batch fermentation of chitosanase from aspergillus fumigatus in escherichia coli.chitosanase (csn) from aspergillus fumigatus has good thermal stability, wide ph range duration, and effective hydrolysis for chitosan. inhere, csn was successfully expressed in escherichia coli followed by extracellular secretion under the guidance of an n-terminal signal peptide pelb, which effectively prompted its secretion out of e. coli cells. to facilitate its later purification, n-terminal or c-terminal 6xhis epitope tag was added to the pelb-csn protein complex. our results indicated tha ...201627368916
fungal epidemiology and diversity in cystic fibrosis patients over a 5-year period in a national reference center.the knowledge on prevalence rates of yeasts and moulds in patients with cystic fibrosis (cf) in germany is scarce. the aim of this report is to give an overview of the diversity and epidemiology of fungal species in cf patients. over a 5-year period, all fungal isolates cultured from microbiological specimen from cf patients were recorded. beside standard bacteriological culture media two fungal media were used for cultivation. species were identified by microscopy, biochemical profiling, maldi- ...201627364649
antimicrobial activity for excretion and secretion of the greenbottle fly larvae lucilia sericata (meigen) (diptera: calliphoridae).sterile larval excretion/secretion (es) exhibited antibacterial activity against some species of bacteria. they were shown to inhibit the growth of gram-positive bacteria staphylococcus aureus and bacillus subtilis gram-negative bacteria pseudomonas aeruginosa, escherichia coli and klebsiella pneumoniae and fungi geotricum candidum and aspergillus fumigatus thus exhibited limited inhibitory effect towards gram-positive bacteria streptococcus pyogenes and staphylococcus epidermidis and gram-negat ...201627363054
biosynthesis of cell wall mannan in the conidium and the mycelium of aspergillus fumigatus.the galactomannan is a major cell wall molecule of aspergillus fumigatus. this molecule is composed of a linear mannan with a repeating unit composed of four α1,6 and α1,2 linked mannose with side chains of galactofuran. to obtain a better understanding of the mannan biosynthesis in a. fumigatus, it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing α1,6 and α1,2 mannose linkages in yeast. these d ...201627603677
a high throughput method for rapid screening of chitosanase-producing fungal strain under acidic conditions.a novel high-throughput method was established for rapid screening of a large numbers of aspergillus fumigatus (a. fumigatus) mutants with high chitosanase production under acidic culture condition by exploiting the fact that iodine can be used as the indicator to stain chitosan but is ineffective for chitooligosaccharides. the mutant population was generated by irradiating a. fumigatus cicc 2434 with co(60)-γ rays. mutants were cultured on acidic plates containing colloidal chitosan and prelimi ...201627628334
the crystal structure of peroxiredoxin asp f3 provides mechanistic insight into oxidative stress resistance and virulence of aspergillus fumigatus.invasive aspergillosis and other fungal infections occur in immunocompromised individuals, including patients who received blood-building stem cell transplants, patients with chronic granulomatous disease (cgd), and others. production of reactive oxygen species (ros) by immune cells, which incidentally is defective in cgd patients, is considered to be a fundamental process in inflammation and antifungal immune response. here we show that the peroxiredoxin asp f3 of aspergillus fumigatus inactiva ...201627624005
dataset of differentially regulated proteins in huvecs challenged with wild type and ugm1 mutant aspergillus fumigatus strains.invasive aspergillosis is the primary opportunistic invasive fungal infection described in neutropenic hematologic patients, caused by the angioinvasive pathogen aspergillus fumigatus. the molecular mechanisms associated with a. fumigatus infection in the vascular endothelium are poorly understood. in this context, we used a high-throughput proteomic approach to unveil the proteins modulated in huvecs after interaction with a wild type strain and the ugm1 mutant (δugm1) of a. fumigatus. the prot ...201627622208
insight into enzymatic degradation of corn, wheat, and soybean cell wall cellulose using quantitative secretome analysis of aspergillus fumigatus.lignocelluloses contained in animal forage cannot be digested by pigs or poultry with 100% efficiency. on contrary, aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase encoding genes, suggesting that a. fumigatus is an efficient lignocellulose degrader. hence the present study uses corn, wheat, or soybean as a sole carbon source to culture a. fumigatus under animal physiological condition to understand how cellulolytic enzymes work together to achi ...201627618962
ligand based pharmacophoric modelling and docking of bioactive pyrazolium 3-nitrophthalate (p3np) on bacillus subtilis, aspergillus fumigatus and aspergillus niger - computational and hirshfeld surface analysis.biologically active lewis acid-base compound, pyrazolium 3-nitro phthalate (p3np) has been synthesized and crystallized by slow evaporation - solution method at 30°c. spectral and single crystal x-ray diffraction (xrd) were used to characterize the compound. the stability of the p3np was confirmed by uv-visible spectral analysis. p3np crystallizes in monoclinic p21/c space group with cell parameters, a=13.009 (3) å, b=12.584 (3) å, c=7.529 (18) å and β=93.052 (4)(o) with z=4. crystal packing was ...201627614246
antifungal activity of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides: a novel type of compound afforded by azide-enolate (3+2) cycloaddition.the first report of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides (7a and 7b) is described herein. azide-enolate (3+2) cycloaddition afforded the synthesis of this novel type of compound. antifungal activity was evaluated in vitro against four filamentous fungi (aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae and mucor hiemalis) as well as nine species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 7a and 7b are promising candidates for co ...201627656774
determination of antifungal susceptibility patterns among the environmental isolates of aspergillus fumigatus in iran.in recent years, triazole-resistant environmental isolates of aspergillus fumigatus have emerged in europe and asia. azole resistance has been reported in patients who are treated with long-term azole therapy or exposure of the fungus spores to the azole fungicides used in agriculture. to date, a wide range of mutations in a. fumigatus have been described conferring azole-resistance, which commonly involves modifications in the cyp51a gene. we investigated antifungal susceptibility pattern of en ...201627656605
the putative flavin carrier family flca-c is important for aspergillus fumigatus virulence.aspergillus fumigatus is an opportunistic fungal pathogen and the most important species causing pulmonary fungal infections. the signaling by calcium is very important for a. fumigatus pathogenicity and it is regulated by the transcription factor crza. we have previously used used chip-seq (chromatin immunoprecipitation dna sequencing) aiming to identify gene targets regulated by crza. we have identified among several genes regulated by calcium stress, the putative flavin transporter, flca. thi ...201627652896
heterogeneity among isolates reveals that fitness in low oxygen correlates with aspergillus fumigatus virulence.previous work has shown that environmental and clinical isolates of aspergillus fumigatus represent a diverse population that occupies a variety of niches, has extensive genetic diversity, and exhibits virulence heterogeneity in a number of animal models of invasive pulmonary aspergillosis (ipa). however, mechanisms explaining differences in virulence among a. fumigatus isolates remain enigmatic. here, we report a significant difference in virulence of two common lab strains, cea10 and af293, in ...201627651366
aspergillus fumigatus photobiology illuminates the marked heterogeneity between isolates.the given strain of aspergillus fumigatus under study varies across laboratories, ranging from a few widely used "standards," e.g., af293 or cea10, to locally acquired isolates that may be unique to one investigator. since experiments concerning physiology or gene function are seldom replicated by others, i.e., in a different a. fumigatus background, the extent to which behavioral heterogeneity exists within the species is poorly understood. as a proxy for assessing such intraspecies variability ...201627651362
scf ubiquitin ligase f-box protein fbx15 controls nuclear co-repressor localization, stress response and virulence of the human pathogen aspergillus fumigatus.f-box proteins share the f-box domain to connect substrates of e3 scf ubiquitin ring ligases through the adaptor skp1/a to cul1/a scaffolds. f-box protein fbx15 is part of the general stress response of the human pathogenic mold aspergillus fumigatus. oxidative stress induces a transient peak of fbx15 expression, resulting in 3x elevated fbx15 protein levels. during non-stress conditions fbx15 is phosphorylated and f-box mediated interaction with skpa preferentially happens in smaller subpopulat ...201627649508
diagnosis of invasive pulmonary aspergillosis.culturing strains from clinical samples is the main method to diagnose invasive pulmonary aspergillosis. detecting the galactomannan antigen in serum samples is an auxiliary examination. the goal of this study was to determine the frequency with which aspergillus fumigatus was cultured in clinical samples taken from patients hospitalized in the the infant jesus teaching hospital in warsaw, poland, in the period of 2013-2014. specimens from the respiratory tract and blood were cultured for mycolo ...201627645542
synthesis, characterization, and biological activity of cross-linked chitosan biguanidine loaded with silver nanoparticles.chitosan biguanidine hydrochloride (chg) and glutaraldehyde cross-linked chitosan biguanidine (cchg) were synthesized and characterized by fourier transform infrared spectroscopy, (1)h nmr and (13)c nmr, x-ray diffraction, scanning electron microscopy (sem) and thermal analyses (tga and dta). the results showed that chg and cchg had a more amorphous structure than that of chitosan, and their thermal stability were slightly lower than that of chitosan. colloidal silver nanoparticles (agnps) were ...201627662448
genotypic discrimination of aspergillus fumigatus strain from related species within section fumigati.the aim was to make an exact diagnosis of 20 strains using molecular biological methods which were isolated from the atmosphere of the inpatient rooms in the oncology and other departments of the ege university medical faculty hospital, and identified as aspergillus fumigatus through phenotypic tests, and to determine their antibiotic susceptibility patterns.201627660866
exposure to positively- and negatively-charged plasma cluster ions impairs ige-binding capacity of indoor cat and fungal allergens.environmental control to reduce the amount of allergens in a living place is thought to be important to avoid sensitization to airborne allergens. however, efficacy of environmental control on inactivation of airborne allergens is not fully investigated. we have previously reported that positively- and negatively-charged plasma cluster ions (pc-ions) reduce the ige-binding capacity of crude allergens from japanese cedar pollen as important seasonal airborne allergens. cat (felis domesticus) and ...201627660668
aspergillus fumigatus dbm 4057 biofilm formation is inhibited by chitosan, in contrast to baicalein and rhamnolipid.the biofilms of filamentous-forming fungi are a novel and still insufficiently understood research topic. we have studied aspergillus fumigatus, an ubiquitous opportunistic pathogenic fungus, as a representative model for a study of biofilm formation by filamentous fungi and for assessing the potential anti-biofilm activity of natural substances. the activity of antibiotic amphotericin b and selected natural substances: baicalein, chitosan and rhamnolipid was studied. the minimum suspension inhi ...201627660214
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