Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| how livestock and flooding mediate the ecological integrity of working forests in amazon river floodplains. | the contribution of working forests to tropical conservation and development depends upon the maintenance of ecological integrity under ongoing land use. assessment of ecological integrity requires an understanding of the structure, composition, and function and major drivers that govern their variability. working forests in tropical river floodplains provide many goods and services, yet the data on the ecological processes that sustain these services is scant. in flooded forests of riverside am ... | 2016 | 27039519 |
| isolation and identification of prepubertal buffalo (bubalus bubalis) spermatogonial stem cells. | isolation and culture of spermatogonial stem cells (sscs) are attractive for production of genetic modified offspring. in the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. to recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. two types of cells, immature sertoli cell and type a spermatogonia were obser ... | 2016 | 26954139 |
| sequence analysis of the s1pr1 gene in river buffalo. | recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. the s1pr1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. here, we performed next generation sequencing in a buffalo bac dna clone and obtained a 54.5-kb sequence encompassing the entire buffalo s1pr1 gene as well as the 27 kb upstream region ... | 2016 | 26909928 |
| investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis). | the aim of this study was to describe modifications in plasma homocysteine (hcy), vitamin e (vite) and malondialdehyde (mda) concentrations in the first 56 days after artificial insemination (ai) in buffalo. thirty-five buffalo cows were divided, ex post, into three groups on the basis of pregnancy diagnosis: pregnant, not pregnant, with embryonic mortality. pregnancy was diagnosed by ultrasonography and plasma concentrations of pregnancy-associated glycoproteins (pags). our results showed that, ... | 2016 | 26850546 |
| rapid detection of water buffalo ricotta adulteration or contamination by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. | the improvement and development of novel technologies and analytical tools are an important first line of defence for both detecting and deterring food frauds. in order to protect the authenticity of traditional foods and safeguard geographical indications and traditional specialities at european level, protected designation of origin (pdo) legislation has been introduced as a guarantee of quality (council regulation ecc 2081/92). dairy products, especially those certified as pdo products, are a ... | 2016 | 26777680 |
| 208 production of transgenic cloned buffalo (bubalus bubalis) embryos containing human insulin gene through hand-guided cloning. | diabetes is a growing disease worldwide and has emerged as a major healthcare problem in india. insulin is an essential medicine for the treatment of diabetes. large dairy animals, such as buffaloes and cows, may be used as bioreactors for cost-effective production of human insulin. the present study was aimed to produce transgenic buffalo embryos containing the human insulin gene through hand-guided cloning for production of transgenic animals. buffalo female fetal fibroblast cells at passage n ... | 2016 | 28279005 |
| 164 carnitine improves post-thawing sperm motility by increasing adenosine triphosphate content in buffalo (bubalus bubalis). | semen cryopreservation plays a critical role for a wide application of both ai and in vitro embryo production in buffalo. in this species, spermatozoa are more susceptible to hazards during freezing and thawing than cattle spermatozoa, thus resulting in lower fertilizing potential (andrabi et al. 2008 anim. reprod. sci. 104, 427-433). carnitine is a quaternary ammonium compound with antioxidant capacities, able to reduce the availability of lipids for peroxidation by transporting fatty acids int ... | 2016 | 28278961 |
| 151 effect of ascorbic acid on oxidative stress and its thermoprotectant role on in vitro embryonic development of buffalo (bubalus bubalis) embryos. | the most important factors that lead to stress in farm animals are oxidative and thermal stress, leading to reduced reproductive efficiency. oxidative stress leads to an increase in proportion of reactive oxygen species, whereas heat stress affects the physiology of animals, which lowers the conception rates of dairy cattle. in vitro culture systems have been enhanced by manipulating media with various supplements such as vitamins, growth factors, and antioxidants that have overcome these proble ... | 2016 | 28278948 |
| 33 buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization differ in their global transcriptome profile. | although the blastocyst rate obtained with nuclear transferred (nt) embryos is higher than that obtained following in vitro fertilization (ivf) in buffalo, the live birth rate of nt embryos is <2% across different farm animal species compared with a birth rate >40% obtained with ivf embryos. this is believed to be due primarily to incomplete or incorrect nuclear reprogramming of the donor somatic cell by the oocyte, which results in aberrant embryonic gene expression. we compared the global tran ... | 2016 | 28278830 |
| 32 next-generation sequencing discloses differences in microrna expression profiles of buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization. | mammalian embryo development is a complex process with a series of critical events taking place at every stage of development. it is an established fact that the birth rate of animals produced by nuclear transfer (nt) is far less (<2%) than that of ivf embryos (40%) after successful embryo transfers in different farm animal species. micro(mi)rnas are small non-coding rnas of 17 to 25 nucleotides that alter the function of their target genes by either degrading them or inhibiting their expression ... | 2016 | 28278829 |
| strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the china/indochina border region. | the swamp type of the asian water buffalo is assumed to have been domesticated by about 4000 years bp, following the introduction of rice cultivation. previous localizations of the domestication site were based on mitochondrial dna (mtdna) variation within china, accounting only for the maternal lineage. we carried out a comprehensive sampling of china, taiwan, vietnam, laos, thailand, nepal and bangladesh and sequenced the mtdna cytochrome b gene and control region and the y-chromosomal zfy, sr ... | 2016 | 26677084 |
| mass spectrometry detection of fraudulent use of cow whey in water buffalo, sheep, or goat italian ricotta cheese. | ricotta cheese is a typical italian product, made with whey from various species, including cow, buffalo, sheep, and goat. ricotta cheese nominally manufactured from the last three species may be fraudulently produced using the comparatively cheaper cow whey. exposing such food frauds requires a reliable analytical method. despite the extensive similarities shared by whey proteins of the four species, a mass spectrometry-based analytical method was developed that exploits three species-specific ... | 2016 | 26675863 |
| molecular characterization of buffalo haptoglobin: sequence based structural comparison indicates convergent evolution between ruminants and human. | haptoglobin (hp) protein has high affinity for hemoglobin (hb) binding during intravascular hemolysis and scavenges the hemoglobin induced free radicals. earlier reports indicate about uniqueness of hp molecule in human and cattle, but in other animals, it is not much studied. in this paper, we characterized buffalo hp molecule and determined its molecular structure, evolutionary importance, and tissue expression. comparative analysis and predicted domain structure indicated that the buffalo hp ... | 2016 | 26646629 |
| molecular identification and seasonal infections of species of fasciola in ruminants from two provinces in china. | we determined the prevalence and seasonality of infections by fasciola of goats and bovine species (cattle and water buffalo) in hubei and anhui provinces of china. faecal samples were collected at 2- to 3-month intervals from 200 goats in hubei province and from 152 bovine species in anhui province. all faecal samples were examined for the presence of parasites. we determined the nucleotide sequences of the first and second internal transcribed spacers (its-1 and its-2) of the nuclear ribosomal ... | 2016 | 26123576 |
| prevalence of antibodies against bubaline herpesvirus (buhv-1) among mediterranean water buffalo (bubalus bubalis) with implications in buffalo trade. | both bovine herpesvirus (bohv-1) and bubaline herpesvirus (buhv-1) have been reported to cross the species barrier. antibody seroconversion in glycoprotein e (ge) blocking elisa during buhv-1 infection has been documented. recent diagnostic efforts have focused on the development and application of discriminatory tests to distinguish between infections with bohv-1 and buhv-1. | 2016 | 27334411 |
| antibodies to bovine viral diarrhoea virus (bvdv) in water buffalo (bubalus bubalis) and cattle from the northern territory of australia. | farmed and feral water buffaloes (bubalus bubalis) populations often coexist with cattle in the northern territory of australia, but their level of exposure to bovine viral diarrhoea virus (bvdv) is unknown. | 2016 | 27785794 |
| molecular cloning, polymorphism, and functional activity of the bovine and water buffalo mx2 gene promoter region. | bovine mx2 gene sequences were already reported, but further information about the gene properties is not yet available. the objective of the current study was to elucidate the structural properties of the bovine mx2 gene mainly the promoter region and its possible functional role. if available, such information would help in assessing the functional properties of the gene, which was reported to confer antiviral action against recombinant vsv. | 2016 | 28066698 |
| exploration of luteinizing hormone in murrah buffalo (bubalus bubalis) urine: extended surge window opens door for estrus prediction. | estrus detection in buffaloes has been a major concern for decades, and lack of reliable methods affects their effective reproductive management. luteinizing hormone (lh) detection in urine is in practice for several mammals for timed insemination, whereas very few reports are available on buffalo urinary lh. the focus of this study is to detect the presence of lh in buffalo urine, quantitate variation in urinary lh during different estrous cycle phases and examine the duration of mid-cycle lh w ... | 2016 | 28011259 |
| protein modeling and molecular dynamics simulation of cloned regucalcin (rgn) gene from bubalus bubalis. | regucalcin (rgn), a calcium regulating protein having anti prolific, anti-apoptotic functions, plays important part in the biosynthesis of ascorbic acid. it is a highly conserved protein that has been reported from many tissue types of various vertebrate species. employing its effect of regulating enzyme activities through reaction with sulfhydryl group (-sh) and calcium, structural level study is believed to offer a better understanding of its binding properties along with information exploited ... | 2016 | 28000561 |
| fatal theileria orientalis n2 genotype infection among asian water buffaloes (bubalus bubalis) in a commercial dairy farm in kerala, india. | fifteen dairy buffaloes of a farm in the state of kerala, india developed fatal oriental theileriosis within 2 months of their procurement. typical piroplasms of theileria orientalis were observed in the erythrocytes of all affected animals by giemsa-leishman staining of blood smears. case fatality rate was 87·5% (seven out of eight) in the clinically progressed cases. therapeutic management with anti-theilerial drugs buparvaquone and oxytetracycline led to recovery of seven other animals in les ... | 2016 | 26522773 |
| trypanosoma vivax in water buffalo of the venezuelan llanos: an unusual outbreak of wasting disease in an endemic area of typically asymptomatic infections. | trypanosoma vivax has been associated with asymptomatic infections in african and south american buffalo. in this study, t. vivax was analyzed in water buffalo (bubalus bubalis) from venezuela in a molecular survey involving 293 blood samples collected from 2006 to 2015 across the llanos region. results demonstrated constant infections (average 23%) during the years analyzed. in general, animals were healthy carriers of t. vivax with low levels of parasitemia and were diagnosed exclusively by tv ... | 2016 | 27884441 |
| short communication: role of streptococcus pluranimalium in mediterranean buffaloes (bubalus bubalis) with different udder health statuses. | the aims of the current study were to describe presence and clinical role over time of streptococcus pluranimalium isolated in milk samples of mediterranean buffalo (mb). two hundred composite milk samples originating from 40 primiparous mb were collected at 10, 30, 60, 90, and 150d in milk (dim) and from 20 pluriparous mb at 77 to 120 dim. milk samples were used for analysis of somatic cell counts, bacteriological cultures, and identification (matrix-assisted laser desorption/ionization time-of ... | 2016 | 26805969 |
| no change in mrna expression of immune-related genes in peripheral blood mononuclear cells challenged with theileria annulata in murrah buffalo (bubalus bubalis). | water buffaloes (bubalus bubalis) act as carrier to theileria annulata and show less clinical sign of tropical theileriosis as compared to indigenous and exotic cattle. differential expression of immune-related genes such as major histocompatibility complex, class ii, dq alpha 1 (mhc-dqα), signal-regulatory protein alpha (sirpa), prion protein (prnp), toll-like receptor 10 (tlr10), c-musculoaponeurotic fibrosarcoma oncogene homolog (cmaf) and v-maf avian musculoaponeurotic fibrosarcoma oncogene ... | 2016 | 26997138 |
| the resurrection of a species: sarcocystis bovifelis heydorn et al., 1975 is distinct from the current sarcocystis hirsuta in cattle and morphologically indistinguishable from sarcocystis sinensis in water buffaloes. | in the mid-1970s, it was established through transmission experiments and ultrastructural studies of sarcocysts by transmission electron microscopy (tem) that cattle was the intermediate host of three sarcocystis spp. using dogs, cats and humans, respectively, as definitive hosts. the cat-transmitted species with microscopic sarcocysts was initially named sarcocystis bovifelis, but it was soon renamed sarcocystis hirsuta, since it was considered to be identical with a previously named species. i ... | 2016 | 26462803 |
| prevalence and first molecular identification of sarcocystis species in cattle and water buffaloes in india. | the importance of sarcocystis hominis in causing zoonotic infections is well known. recently, s. hominis like cysts have been reported from water buffalo in china. previous studies indicate prevalence of sarcocystis species in bovine populations in india but molecular evidence is required for proper species differentiation. we examined two hundred and ninety six cardiac tissue samples of indian water buffaloes and cattle from northern and western parts of the country. tissues were examined for s ... | 2016 | 27447215 |
| sarcocystis rommeli, n. sp. (apicomplexa: sarcocystidae) from cattle (bos taurus) and its differentiation from sarcocystis hominis. | cattle (bos taurus) are intermediate hosts for three named species of sarcocystis, s. cruzi, s. hirsuta, and s. hominis. recently, a fourth species was identified and named s. sinensis. however, s. sinensis originally named a species of sarcocystis in water buffalo (bubalus bubalis) in china. based on unverifiable evidence, it was suggested that the same parasite infects cattle. in addition, s. sinensis was recently declared as nomen nudum because its naming violated the rules of international c ... | 2016 | 26111603 |
| combined il-12 plasmid and recombinant sjgst enhance the protective and anti-pathology effect of sjgst dna vaccine against schistosoma japonicum. | schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. development of a vaccine is thought to be the most effective way to control this disease. recombinant 26-kda glutathione s-transferase (rsjgst) has previously been reported to achieve a worm reduction rate of 42-44%. to improve the efficiency of the vaccine against schistosoma japonicum, we immunized mice with a combination of pcdna vector-encoded 26-kda sjgst (pcdn ... | 2016 | 26891172 |
| changes in the physico-chemical and microbial quality during the production of pastırma cured with different levels of sodium nitrite. | pastırma, a dry-cured meat product, is produced from the whole muscle and/or muscles obtained from certain parts of beef and water buffalo carcasses. the purpose of this study was to determine the effects of different levels of sodium nitrite on changes in the physicochemical and microbial quality during the production of pastırma. the changes in residual nitrite, salt, ph, moisture, thiobarbutiric acid reactive substances (tbars), colour (l*, a*, b*), total aerobic mesophilic bacteria (tamb), l ... | 2016 | 27857537 |
| metabolomics of the antipyretic effects of bubali cornu (water buffalo horn) in rats. | bubali cornu (water buffalo horn, wbh) has been used for thousands of years in traditional chinese medicine (tcm) as an effective treatment for heat. in the present study, we have carried out a metabolomics profiling study on plasma and urine samples to explore potential biomarkers and determine how wbh exerts its antipyretic effects in yeast-induced pyrexia at a metabolomic level. ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (uplc- ... | 2016 | 27384078 |
| complete genome sequence of a lytic siphoviridae bacteriophage infecting several serovars of salmonella enterica. | the bacteriophage 100268_sal2 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against several subspecies of salmonella enterica this bacteriophage belongs to the siphoviridae family and has a 125,114-bp double-stranded dna (ds-dna) genome containing 188 coding sequences (cdss). | 2016 | 27688334 |
| complete genome sequences of three siphoviridae bacteriophages infecting salmonella enterica serovar enteritidis. | three bacteriophages, 118970_sal1, 118970_sal2, and 64795_sal3, were isolated from water buffalo feces in southern italy, exhibiting lytic activity against salmonella enterica serovar enteritidis. these bacteriophages belong to the siphoviridae family and have a 60,113-bp, 123,930-bp, and 48,094-bp double-stranded dna (dsdna) genome containing 72, 173, and 80 coding sequences (cdss), respectively. | 2016 | 27856572 |
| molecular differentiation of sarcocystis buffalonis and sarcocystis levinei in water buffaloes (bubalus bubalis) from sarcocystis hirsuta and sarcocystis cruzi in cattle (bos taurus). | the purpose of the present study was to obtain sarcocysts of sarcocystis buffalonis and sarcocystis levinei from water buffaloes and characterize the isolates by molecular methods in order to determine whether the two species were genetically different from sarcocystis hirsuta and sarcocystis cruzi, respectively, from cattle, which had been characterized before. about 35 macroscopically visible (3-4 × 1-2 mm) and 20 barely visible (1-3 × 0.2 mm) sarcocysts were excised from the esophagus of 18 n ... | 2016 | 26979729 |
| molecular characterisation of sarcocystis bovifelis, sarcocystis bovini n. sp., sarcocystis hirsuta and sarcocystis cruzi from cattle (bos taurus) and sarcocystis sinensis from water buffaloes (bubalus bubalis). | about 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries (argentina, brazil, germany, new zealand, uruguay) and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology. genomic dna was extracted from 147 of these sarcocysts and used initially for pcr amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit i gene (cox1) in order to identify the sarcocysts to species and/or se ... | 2016 | 26677095 |
| domestic cats (felis catus) are definitive hosts for sarcocystis sinensis from water buffaloes (bubalus bubalis). | the definitive hosts of sarcocystis sinensis in water buffaloes have hitherto been unknown, but the close similarity of this species to the cat-transmitted sarcocystis bovifelis in cattle suggested they were felids. in a previous study, two domestic cats were fed macroscopic sarcocysts of sarcocystis fusiformis contained within or dissected from the esophageal muscles of water buffaloes, while no microscopic sarcocysts of s. sinensis were noticed. both cats started shedding small numbers of spor ... | 2016 | 27075117 |
| involvement of the nervous system following experimental infection with pasteurella multocida b:2 in buffalo (bubalus bubalis): a clinicopathological study. | haemorrhagic septicaemia (hs) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of pasteurella multocida b:2 and e:2 in asian and african, respectively. it is well known that hs affect mainly the respiratory and digestive tracts. however, involvement of the nervous system in pathogenesis of hs has been reported in previous studies without details. in this study, nine buffalo calves of 8 months old were distributed into three groups. animals o ... | 2016 | 26850845 |
| paratuberculosis in buffaloes in northeast brazil. | several farms in the northeast of brazil were investigated for mycobacterium avium subsp. paratuberculosis infection in order to identify the occurrence of paratuberculosis in buffaloes. samples were obtained from 17 farms, two slaughter houses, and a quarantine area in the northeast. about 15,000 buffaloes of the murrah, mediterranean, and jafarabadi breed as well as their crossbreeds were evaluated for meat, dairy, and mixed farms with semi-intensive or extensive breeding practices. for diagno ... | 2016 | 27334632 |
| molecular detection of mycobacterium tuberculosis in cattle and buffaloes: a cause for public health concern. | tuberculosis is a re-emerging disease causing a growing public health burden. the current study was conducted to investigate the occurrence of mycobacterium tuberculosis among cattle and buffaloes with tuberculous lesions. typical tuberculous lesions were collected from 34 cattle and 34 buffaloes (bubalus bubalis) through postmortem examination of slaughtered animals in abattoirs. dnas were extracted from samples, and m. tuberculosis was identified by pcr. positive samples were examined for resi ... | 2016 | 27517579 |
| molecular characterization and phylogenetic analysis of fasciola gigantica from western java, indonesia. | fasciola gigantica and aspermic (hybrid) fasciola flukes are thought to be distributed in southeast asian countries. the objectives of this study were to investigate the distribution of these flukes from unidentified ruminants in western java, indonesia, and to determine their distribution history into the area. sixty fasciola flukes from western java were identified as f. gigantica based on the nucleotide sequences of the nuclear phosphoenolpyruvate carboxykinase (pepck) and dna polymerase delt ... | 2016 | 27266482 |
| rumen adaptation of swamp buffaloes (bubalus bubalis) by high level of urea supplementation when fed on rice straw-based diet. | four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly allocated to investigate rumen adaptation of urea on feed intake, nutrient digestibility, fermentation efficiency, and microbial protein synthesis. buffaloes were fed with rice straw ad libitum for a period of 2 weeks and then were shifted to a step-up diet regimen by supplementation of concentrate containing 20 and 40 g/kg urea at 5 g/kg bw for a period of 2 and 4 weeks, respectively. the results revealed that feed intake and ... | 2016 | 27107749 |
| intraspecific and interspecific genetic variation of gongylonema pulchrum and two rodent gongylonema spp. (g. aegypti and g. neoplasticum), with the proposal of g. nepalensis n. sp. for the isolate in water buffaloes from nepal. | the gullet worm (gongylonema pulchrum) has been recorded from a variety of mammals worldwide. in an earlier study, we demonstrated two separate transmission cycles in cattle (bos taurus) and wild mammals in japan based on nucleotide sequences of the ribosomal rna gene (rdna) and cytochrome c oxidase subunit i (cox-1) region of mitochondrial dna of multiple isolates of different origins. our earlier study additionally demonstrated two major cox-1 haplotypes of g. pulchrum prevalent in cattle in j ... | 2016 | 26531300 |
| inhibitory potential of buffalo (bubalus bubalis) colostrum immunoglobulin g on klebsiella pneumoniae. | the unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. in this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin g on human pathogen klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. the ... | 2016 | 27017977 |
| influence of cutting and deboning operations on the microbiological quality and shelf life of buffalo meat. | considering the specific biochemical composition of buffalo (bubalus bubalis) meat (high iron content, high biological value proteins and essential fatty acids, low amounts of fat and cholesterol), we evaluated the influence of cutting and deboning operations on the microbiological quality and shelf-life of vacuum-packed buffalo meat stored under refrigeration. on the processing day, samples were collected from carcass, deboning room surfaces and meat cuts. samples from meat cuts were evaluated ... | 2016 | 26897086 |
| g-protein-coupled chemokine receptor gene in lumpy skin disease virus isolates from cattle and water buffalo (bubalus bubalis) in egypt. | lumpy skin disease virus (lsdv), sheep poxvirus (spv) and goat poxvirus (gpv) are the most serious poxviruses of ruminants. in this study, we analysed the g-protein-coupled chemokine receptor (gpcr) genes of lsdv isolates from cattle and water buffalo (bubalus bubalis) in egypt during the summer of 2011. multiple alignments of the nucleotide sequences revealed that the water buffalo lsdv isolate differed from the cattle isolate at four nucleotide positions, and both isolates had nine nucleotide ... | 2016 | 25754131 |
| 2d-page analysis of the soluble proteins of the tropical liver fluke, fasciola gigantica and biliary amphistome, gigantocotyle explanatum, concurrently infecting bubalus bubalis. | the digenetic trematodes, fasciola gigantica and gigantocotyle explanatum, belonging to the family fasciolidae and paramphistomidae respectively, have been often found to concurrently infect the liver of indian water buffalo bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. in the present study the soluble gene products of both f. gigantica and g. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. the soluble prote ... | 2016 | 27605808 |
| detection of campylobacter in human and animal field samples in cambodia. | campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with campylobacter jejuni and c. coli being the most commonly detected species. the aim of this study was to detect campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex pcr in faecal samples frozen before analysis. of 681 human samples, 82 (12%) tested positive by pcr (c. jejuni in 66 samples an ... | 2016 | 26991032 |
| first genome sequences of buffalo coronavirus from water buffaloes in bangladesh. | we report the complete genome sequences of a buffalo coronavirus (bufcov hku26) detected from the faecal samples of two domestic water buffaloes (bubalus bubalis) in bangladesh. they possessed 98-99% nucleotide identities to bovine coronavirus (bcov) genomes, supporting bufcov hku26 as a member of betacoronavirus 1. nevertheless, bufcov hku26 possessed distinct accessory proteins between spike and envelope compared to bcov. sugar-binding residues in the n-terminal domain of s protein in bcov are ... | 2016 | 27274850 |
| risk analysis and seroprevalence of bovine ephemeral fever virus in cattle in the kingdom of saudi arabia. | bovine ephemeral fever virus (befv) is an arthropod-borne rhabdovirus that causes disabling clinical signs and major economic losses in cattle and water buffalo. the disease is well documented in asia, africa, and the middle east; however, the seroprevalence of befv in different regions and bovine breeds in the kingdom of saudi arabia (ksa) is unknown. the aim of this study was to analyze risk factors which affect the prevalence of antibodies against befv in small herds of cattle in four geograp ... | 2016 | 26676243 |
| proteomic based approach for characterizing 4-hydroxy-2-nonenal induced oxidation of buffalo (bubalus bubalis) and goat (capra hircus) meat myoglobins. | myoglobin (mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (hne) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of mb and predispose it to greater oxidation. however, no literature is available on characterization of lipid oxidation induced oxidation of indian water buffalo (bubalus bubalis) and goat (capra hircus) myoglobins. | 2016 | 27891064 |
| molecular assays reveal the presence of theileria spp. and babesia spp. in asian water buffaloes (bubalus bubalis, linnaeus, 1758) in the amazon region of brazil. | approximately 50% of buffalo herds in brazil are located in pará state in northern brazil. there are several properties where cattle and buffalo live and graze together, and thus, buffalo pathogens may threaten the health of cattle and vice versa. therefore, knowledge of infectious agents of buffalo is essential for maintaining healthy livestock. clinical disease caused by theileria and babesia parasites in the asian water buffalo is not common, although these animals may act as reservoir hosts, ... | 2016 | 27344507 |
| epidemiology of bovine hemoprotozoa parasites in cattle and water buffalo in vietnam. | a pcr-based survey of hemoprotozoa parasites detected babesia bigemina, theileria orientalis and trypanosoma theileri among cattle and water buffalo in vietnam, and a new babesia sp. closely related to babesia ovata was detected in cattle only. in addition, theileria annulata and trypanosoma evansi were not detected in both cattle and water buffalo. phylogenetic analysis detected t. orientalis mpsp genotypes 3, 5, 7 and n3 in cattle and 5, 7, n1 and n2 in water buffalo. additionally, water buffa ... | 2016 | 27149894 |
| cattle are a potential reservoir of bubaline herpesvirus 1 (buhv1). | in the present work, controlled experimental infection and transmission studies in domestic cattle (bos taurus) and water buffaloes (bubalus bubalis) were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (buhv1). two bovine and two buffalo calves were infected with buhv1 (20287n isolate) by intranasal aerosolisation. two sentinel cattle did not receive the virus challenge, but were housed with infected buffaloes to evaluate horizontal transmission. all experimentally inoculat ... | 2016 | 28074144 |
| lessons for livestock genomics from genome and transcriptome sequencing in cattle and other mammals. | decreasing sequencing costs and development of new protocols for characterizing global methylation, gene expression patterns and regulatory regions have stimulated the generation of large livestock datasets. here, we discuss experiences in the analysis of whole-genome and transcriptome sequence data. | 2016 | 27534529 |
| comparative analysis of snp candidates in disparate milk yielding river buffaloes using targeted sequencing. | river buffalo (bubalus bubalis) milk plays an important role in economy and nutritious diet in several developing countries. however, reliable milk-yield genomic markers and their functional insights remain unexposed. here, we have used a target capture sequencing approach in three economically important buffalo breeds namely: banni, jafrabadi and mehsani, belonging to either high or low milk-yield group. blood samples were collected from the milk-yield/breed balanced group of 12 buffaloes, and ... | 2016 | 27441113 |
| identification of the microrna repertoire in tlr-ligand challenged bubaline pbmcs as a model of bacterial and viral infection. | in the present study, we used high-throughput sequencing, mirna-seq, to discover and explore the expression profiles of known and novel mirnas in tlr ligand-stimulated vis-à-vis non-stimulated (i.e. control) peripheral blood mononuclear cells (pbmcs) isolated from blood of healthy murrah buffaloes. six small rna (srna) libraries were multiplexed in ion torrent pi chip and sequenced on ion proton system. the reads obtained were aligned to the bos taurus genome (umd3.1 assembly), which is phylogen ... | 2016 | 27257788 |
| ungulate malaria parasites. | haemosporida parasites of even-toed ungulates are diverse and globally distributed, but since their discovery in 1913 their characterization has relied exclusively on microscopy-based descriptions. in order to bring molecular approaches to bear on the identity and evolutionary relationships of ungulate malaria parasites, we conducted plasmodium cytb-specific nested pcr surveys using blood from water buffalo in vietnam and thailand, and goats in zambia. we found that plasmodium is readily detecta ... | 2016 | 26996979 |
| de novo transcriptome assembly of the chinese swamp buffalo by rna sequencing and ssr marker discovery. | the chinese swamp buffalo (bubalis bubalis) is vital to the lives of small farmers and has tremendous economic importance. however, a lack of genomic information has hampered research on augmenting marker assisted breeding programs in this species. thus, a high-throughput transcriptomic sequencing of b. bubalis was conducted to generate transcriptomic sequence dataset for gene discovery and molecular marker development. illumina paired-end sequencing generated a total of 54,109,173 raw reads. af ... | 2016 | 26766209 |
| seroprevalence of foot-and-mouth disease in susceptible wildlife in israel. | foot-and-mouth disease (fmd) epidemics recur in israel almost every year. wild even-toed ungulates are seldom affected during these epidemics. the seroprevalence of fmd in wild ungulates during 2000 and 2005-2013 was estimated using anti-non-structural proteins elisa. overall, 209 samples were tested, comprising sera of 120 wild boar (sus scrofa lybicus), 64 mountain gazelles (gazella gazella gazella), 6 water buffaloes (bubalus bubalis), and 19 persian fallow deer (dama dama mesopotamica). none ... | 2016 | 27200364 |
| clinical, laboratory and ultrasonographic findings in egyptian buffalo (bubalus bubalis) with caecal and colonic dilatation. | the present study was conducted to describe the clinical, laboratory and ultrasonographic findings of caecal and colonic dilatation in egyptian buffalo (bubalus bubalis). a total number of forty buffaloes were included in the study and divided into two groups: control group (n = 20) and diseased group (n = 20). diseased buffalo were admitted to the veterinary teaching hospital at assiut university-egypt. each of the diseased animals was subjected to clinical, rectal, laboratory and ultrasonograp ... | 2016 | 30255032 |
| determination of naturally occurring estrogenic hormones in cow's and river buffalo's meat by hplc-fld method. | this study was performed to measure and compare the levels of steroid hormones [estrone (e1), 17β-estradiol (e2), and estriol (e3)] and their conjugated metabolites in cow's and river buffalo's meat in two distinct follicular and luteal phases. moreover, the possible effect of a heating process on steroid hormone concentration was also investigated. the collected meat (biceps femoris muscle) samples were subjected to liquid extraction, enzymatical deconjugation, and c18 solid-phase extraction. e ... | 2016 | 28911549 |
| differences in developmental competence and gene expression profiles between buffalo (bubalus bubalis) preimplantation embryos cultured in three different embryo culture media. | the objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. after ivm/ivf presumptive zygotes were cultured in one of three systems: undefined tcm-199, mcr2aa medium supplemented with 10 % fbs and defined pva-myo-inositol-phosphate-egf medium. no (p > 0.05) differences at 2-cell, 4-cell and 8-cell to 16- cell stages were observed among the three cultured medi ... | 2016 | 27481470 |
| genetic association between snps in the dgat1 gene and milk production traits in murrah buffaloes. | this study identified polymorphisms in the dgat1 gene in murrah buffaloes and investigated the associations to milk production and quality traits (milk, fat and protein yields and percentages, somatic cell count). genomic dna was extracted from hair follicles collected from the tail of 196 females. three snps were identified in dgat1 gene by sequencing. statistical analyses were performed to verify the linkage and the association between polymorphisms and traits. the estimated value of r (2) bet ... | 2016 | 27469895 |
| l-cysteine improves antioxidant enzyme activity, post-thaw quality and fertility of nili-ravi buffalo (bubalus bubalis) bull spermatozoa. | the effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, post-thaw quality parameters and in vivo fertility of nili-ravi buffalo bull spermatozoa were studied. semen samples from 4 buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of l-cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 mm) and frozen in 0.5-ml french straws. the antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase ... | 2016 | 26762772 |
| first report of cryptosporidium species in farmed and wild buffalo from the northern territory, australia. | a molecular epidemiological survey of cryptosporidium from water buffalo (bubalus bubalis) in the northern territory in australia was conducted. fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18s quantitative pcr (qpcr). positives were typed by sequence analysis of 18s nested pcr products. the qpcr prevalence of cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. sequence analysis identified two species: c. pa ... | 2016 | 26758449 |
| buffalo (bubalus bubalis) scnt embryos produced from somatic cells isolated from frozen-thawed semen: effect of trichostatin a on the in vitro and in vivo developmental potential, quality and epigenetic status. | this study examined the effects of trichostatin a (tsa) treatment of reconstructed buffalo embryos, produced by hand-made cloning using somatic cells isolated from over a decade old frozen-thawed semen, on their in vitro and in vivo developmental competence, quality and epigenetic status. following treatment of reconstructed embryos with tsa (0, 50 or 75 nm) for 10 h prior to culture, the cleavage (100.0 ± 0, 94.5 ± 2.3 and 96.1 ± 1.2%, respectively) and blastocyst rate (50.6 ± 2.3, 48.4 ± 2.7 a ... | 2016 | 26503476 |
| 209 production of buffalo (bubalus bubalis) embryos containing human lysozyme gene. | lysozyme is a ubiquitous enzyme found in all major taxa of living organisms with a diverse role in human and animal health. it plays an important role as a nonspecific immune factor and anti-inflammatory factor and is a part of the innate immune system. research on the expression of recombinant human lysozyme is, thus, potentially valuable to the dairy industry. therefore, the present study was carried out to observe the developmental competence and quality of cloned embryos containing the human ... | 2016 | 28279006 |
| 198 successful transplantation of buffalo (bubalus bubalis) germ cells to homologous recipients. | transplantation of isolated germ cells from a fertile donor male into the seminiferous tubules of infertile recipients can result in donor germ cells-derived sperm production. this technique has the potential to be used as an alternative strategy for producing transgenic livestock with higher efficiency and less time and capital requirement than the current methods. the objective of the present study was to investigate whether the homologous transplantation of isolated buffalo germ cells could r ... | 2016 | 28278995 |
| 121 three-dimensional assessment of early corpus luteum vascularity in buffalo (bubalus bubalis). | the aim of the study was to develop an objective method to assess the vascular flow to the early corpus luteum (cl) in buffaloes using colour doppler ultrasound data. our hypothesis was that 3-dimensional (3d) volumetric analysis of vascularity would demonstrate lower variability between animals compared with conventional 2-dimensional (2d) analysis of single images. wave emergence and ovulation was synchronized in buffalo (n=16) using prostaglandin-gnrh based protocols. colour doppler ultrasono ... | 2016 | 28278918 |
| the effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using mehsani buffalo (bubalus bubalis). | in developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. it is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. a metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic mehsani buffalo (bubalus bubalis) fed with varying proportions of roughage. the ex ... | 2016 | 26711277 |
| amnion epithelial cells of buffalo (bubalus bubalis) term placenta expressed embryonic stem cells markers and differentiated into cells of neurogenic lineage in vitro. | aim of the present study was the isolation, culture, and characterization of amniotic membrane-derived epithelial cells (ae) from term placenta collected postpartum in buffalo. we found that cultured cells were of polygonal in shape, resistance to trypsin digestion and expressed cytokeratin-18 indicating that they were of epithelial origin. these cells have negative expression of mesenchymal stem cell markers (cd29, cd44, and cd105) and positive for pluripotency marker (oct4) genes indicated tha ... | 2016 | 26670951 |
| differential developmental competence and gene expression patterns in buffalo (bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells. | the developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (bubalus bubalis) nuclear transfer (nt) embryos from fetal fibroblasts (ffs), amnion mesenchymal stem cells (amscs) and in vitro fertilized (ivf) embryos were compared in the present studies. the in vitro expanded buffalo ffs showed a typical "s" shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. the in vitro cultured undifferentiated amscs showed a doubling t ... | 2016 | 26660476 |
| trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in nili ravi buffaloes (bubalus bubalis). | our objectives were to study the effect of trehalose in extender on (1) antioxidant enzymes profile during cryopreservation (after dilution, before freezing, and after thawing), (2) in vitro quality (after thawing), and (3) in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 20) from four buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60 mm) and frozen in french straws. at ... | 2016 | 26653954 |
| dispositions of enrofloxacin and its major metabolite ciprofloxacin in thai swamp buffaloes. | given the limited information available in this species, the aim of this study was to investigate the pharmacokinetic characteristics of enrofloxacin (er) and its major metabolite ciprofloxacin (cp) in buffaloes, bubalus bubalis. er was administered intravenously (i.v.) or subcutaneously (s.c.) to buffaloes at doses of 5.0 and 7.5 mg/kg bw, and plasma, urine and fecal samples were collected until 48 hr post-administration. the concentrations of er and cp in the plasma, urine and feces were analy ... | 2016 | 26596287 |
| supplementation of slow-release melatonin improves recovery of ovarian cyclicity and conception in summer anoestrous buffaloes (bubalus bubalis). | the role of melatonin as a protective neurohormone against restoring cyclicity in summer anoestrous animals in photoperiod species has gained wider acceptance. this study was designed to uncover the evidence the slow-release melatonin (mlt) has on initiation of ovarian cyclicity and conception rate (cr) in summer anoestrous buffaloes. thus, buffaloes diagnosed as summer anoestrous (absence of overt signs of oestrus, concurrent rectal examination and radioimmunoassay for serum progesterone at 10 ... | 2016 | 26566713 |
| the rediscovery of malaria parasites of ungulates. | over a hundred years since their first description in 1913, the sparsely described malaria parasites (genus plasmodium) of ungulates have been rediscovered using molecular typing techniques. in the span of weeks, three studies have appeared describing the genetic characterization and phylogenetic analyses of malaria parasites from african antelope (cephalophus spp.) and goat (capra aegagrus hircus), asian water buffalo (bubalus bubalis), and north american white-tailed deer (odocoileus virginian ... | 2016 | 27444556 |
| evaluation of leptin receptor expression on buffalo leukocytes. | experimental evidences support a direct role for leptin in immunity. besides controlling food intake and energy expenditure, leptin was reported to be involved in the regulation of the immune system in ruminants. the aim of this work was to highlight the expression of leptin receptor (lepr) on bubalus bubalis immune cells using a multi-approach assessment: flow cytometry, confocal microscopy and gene expression analysis. flow cytometric analysis of lepr expression showed that peripheral blood mo ... | 2016 | 27436440 |
| production of hand-made cloned buffalo (bubalus bubalis) embryos from non-viable somatic cells. | use of non-viable somatic cells for hand-made cloning (hmc) can enable production of cloned animals from tissues obtained from elite or endangered dead animals. buffalo skin fibroblast cells were rendered non-viable by heat treatment and used for hmc. although fusion (93.6 ± 1.72 vs 67.1 ± 2.83%) and cleavage (90.3 ± 1.79 vs 65.8 ± 1.56%) rate was lower (p < 0.001) than that for controls, blastocysts could be successfully produced. however, blastocyst rate (34.1 ± 2.43 vs 6.9 ± 2.18%, p < 0.001) ... | 2016 | 27422686 |
| synonymous single nucleotide polymorphisms in the mc4r gene that are significantly associated with milk production traits in water buffaloes. | melanocortin-4 receptor (mc4r) is associated with feed intake, growth, fatness, and carcass composition in many domestic animals. the aim of this study was to evaluate the association of single nucleotide polymorphisms (snps) in mc4r with milk production traits in water buffalo. eight snps were identified by direct polymerase chain reaction sequencing of samples from 18 buffaloes. snps were then genotyped using the matrix-assisted laser desorption/ionization time of flight mass spectrometry (mal ... | 2016 | 27420995 |
| effect of melatonin on maturation capacity and fertilization of nili-ravi buffalo (bubalus bubalis) oocytes. | this study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (ivm) and in vitro fertilization (ivf) rate of buffalo oocytes. cumulus oocytes complexes (cocs) were aspirated from follicles of 2-8 mm diameter. in experiment i, cocs were matured in ivm medium supplemented with 0 (control), 250, 500, and 1000 μm melatonin for 22-24 hours in co2 incubator at 38.5°c with 5% co2 and at 95% relative humidity. the maturation rate did not differ in media ... | 2016 | 27540514 |
| effect of growth differentiation factor-9 (gdf-9) on the progression of buffalo follicles in vitrified-warmed ovarian tissues. | to improve the reproductive performance of water buffalo to level can satisfy our needs, the mechanisms controlling ovarian follicular growth and development should be thoroughly investigated. therefore, in this study, the expressions of growth differentiation factor-9 (gdf-9) in buffalo ovaries were examined by immunohistochemistry, and the effects of gdf-9 treatment on follicle progression were investigated using a buffalo ovary organ culture system. frozen-thawed buffalo ovarian follicles wit ... | 2016 | 27554536 |
| computational studies on receptor-ligand interactions between novel buffalo (bubalus bubalis) nucleotide-binding oligomerization domain-containing protein 2 (nod2) variants and muramyl dipeptide (mdp). | nucleotide binding and oligomerization domain 2 (nod2), a member of intracellular nod-like receptors (nlrs) family, recognizes the bacterial peptidoglycan, muramyl dipeptide (mdp) and initiates host immune response. the precise ligand recognition mechanism of nod2 has remained elusive, although studies have suggested leucine rich repeat (lrr) region of nod2 as the possible binding site of mdp. in this study, we identified multiple transcripts of nod2 gene in buffalo (bunod2) and at least five lr ... | 2016 | 26897084 |
| identification of polymorphism in fatty acid binding protein 3 (fabp3) gene and its association with milk fat traits in riverine buffalo (bubalus bubalis). | the fatty acid binding protein 3 (fabp3) gene, known to be associated with fat percentage of milk and meat in bovines, was screened among swamp and riverine buffaloes for polymorphism detection and further association with milk fat contents. an snp g.307c > t was identified in the intron 2 (+53 exon 2) region of fabp3 gene of indian buffaloes. the snp identified was genotyped in 692 animals belonging to 15 riverine, swamp and hybrid (riverine × swamp) buffalo populations of diverse phenotypes an ... | 2016 | 26894500 |
| isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (bubalus bubalis) bull spermatozoa. | this study was designed to investigate the occurrence of bacterial species in water buffalo semen at the time of collection/processing and to assess the efficacy of some selected antibiotics (gtls; gentamycin, tylosin and linco-spectin or sp; streptomycin and penicillin) in cryodiluent on bacterial control and quality including in vivo fertility of buffalo spermatozoa. for this purpose, four experiments were conducted. in experiment 1, a total of 11 bacterial species were isolated from buffalo e ... | 2016 | 26892806 |
| caspase-9 inhibitor z-lehd-fmk enhances the yield of in vitro produced buffalo (bubalus bubalis) pre-implantation embryos and alters cellular stress response. | the present investigation was done to study the effect of caspase-9 inhibitor z-lehd-fmk, on in vitro produced buffalo embryos. z-lehd-fmk is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival. buffalo ovaries were collected from slaughterhouse and the oocytes were subjected to in vitro maturation (ivm), in vitro fertilization (ivf) and in vitro culture (ivc). the culture medium was supplemented with z-lehd-fmk at different concentrations i ... | 2016 | 26850530 |
| comparative proteomic analysis of mature and immature oocytes of the swamp buffalo (bubalus bubalis). | maternal protein components change markedly during mammalian oogenesis. many of these proteins have yet to be characterized and verified. in this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the swamp buffalo (bubalus bubalis). proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. image analysis indicated that 17 proteins were differentially express ... | 2016 | 26784167 |
| heat stress and antioxidant enzyme activity in bubaline (bubalus bubalis) oocytes during in vitro maturation. | in vitro environments like heat stress usually increase the production of reactive oxygen species in bubaline oocytes which have been implicated as one of the major causes for reduced developmental competence. oocytes during meiotic maturation are sensitive to oxidative stress, and heat stress accelerates cellular metabolism, resulting in the higher production of free radicals. therefore, the aim of present work was to assess the impact of heat stress during meiotic maturation on bubaline cumulu ... | 2016 | 26781547 |
| comparison of non-linear models to describe the lactation curves for milk yield and composition in buffaloes (bubalus bubalis). | in order to describe the lactation curves of milk yield (my) and composition in buffaloes, seven non-linear mathematical equations (wood, dhanoa, sikka, nelder, brody, dijkstra and rook) were used. data were 116,117 test-day records for my, fat (fp) and protein (pp) percentages of milk from the first three lactations of buffaloes which were collected from 893 herds in the period from 1992 to 2012 by the animal breeding center of iran. each model was fitted to monthly production records of dairy ... | 2016 | 26354679 |
| optixcell improves the postthaw quality and fertility of buffalo bull sperm. | the present study was conducted to compare the liposome-containing, animal protein-free, commercially available optixcell extender with the tris-citric-egg yolk extender for postthaw quality and fertility of buffalo semen. semen was collected from five adult nili-ravi buffalo (bubalus bubalis) bulls of similar age group with an artificial vagina (at 42 °c) for 3 weeks (replicates). semen ejaculates from each buffalo bull were divided into two aliquots and diluted (at 37 °c having 50 × 10(6) sper ... | 2016 | 26522702 |
| treatment of buffalo (bubalus bubalis) donor cells with trichostatin a and 5-aza-2'-deoxycytidine alters their growth characteristics, gene expression and epigenetic status and improves the in vitro developmental competence, quality and epigenetic status of cloned embryos. | we examined the effects of treating buffalo skin fibroblast donor cells with trichostatin a (tsa), a histone deacetylase (hdac) inhibitor, and 5-aza-2'-deoxycytidine (5azadc), a dna methyltransferase (dnmt) inhibitor, on the cells and embryos produced by hand-made cloning. treatment of donor cells with tsa or 5azadc resulted in altered expression levels of the hdac1, dnmt1, dnmt3a, p53, caspase3 and caspase9 genes and global levels of acetylation of lysine at position 9 or 14 in histone 3 (h3k9/ ... | 2016 | 25409339 |
| a comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (bubalus bubalis) embryos. | the efficiency of two cell types, namely adult fibroblasts, and amniotic fluid stem (afs) cells as nuclear donor cells for somatic cell nuclear transfer by hand-made cloning in buffalo (bubalus bubalis) was compared. the in vitro expanded buffalo adult fibroblast cells showed a typical "s" shape growth curve with a doubling time of 40.8 h and stained positive for vimentin. the in vitro cultured undifferentiated afs cells showed a doubling time of 33.2 h and stained positive for alkaline phosphat ... | 2016 | 25501536 |
| assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes. | the present study was designed to investigate the sperm damages occurring in acrosome, plasma membrane, mitochondrial activity, and dna of fresh, equilibrated and frozen-thawed buffalo semen by fluorescent probes. the stability of sperm acrosome and plasma membrane stability, mitochondrial activity and dna status were assessed by fluorescein conjugated lectin pisum sativum agglutinin, annexin-v/propidium iodide, jc-1 and tunel assay, respectively, under the fluorescent microscope. the damages pe ... | 2016 | 25373338 |
| role of linker histone h1c during the reprogramming of chinese swamp buffalo (bubalus bubalis) embryos produced by somatic cell nuclear transfer. | during reprogramming, there is exchange of histone h1c and the oocyte-specific linker histone, and h1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (scnt). the aim of the present study was to investigate the role of the h1c gene in scnt reprogramming in chinese swamp buffalo (bubalus bubalis) using rna interference (rnai). chinese swamp buffalo h1c gene sequences were obtained and h1c-rnai vectors were designed, synthesised and then transfect ... | 2016 | 25145348 |
| igf-1 attenuates lps induced pro-inflammatory cytokines expression in buffalo (bubalus bubalis) granulosa cells. | interaction between immune and endocrine system is a diverse process influencing cellular function and homeostasis in animals. negative energy balance (neb) during postpartum period in dairy animals usually suppresses these systems resulting in reproductive tract infection and infertility. these negative effects could be due to competition among endocrine and immune signaling pathways for common signaling molecules. the present work studied the effect of igf-1 (50 ng/ml) on lps (1 μg/ml) mediate ... | 2015 | 25433435 |
| characterization of leptin receptor gene in bubalus bubalis and association analysis with body measurement traits. | leptin has a pleiotropic effect on regulating appetite, energy metabolism, growth, reproduction, body composition and immunity. this property supports leptin and its receptor as candidate genes for evaluating genetic polymorphisms to associate with growth, milk yield and other economic traits. the aim of this study is to characterize the leptin receptor gene in bubalus bubalis, to identify single-nucleotide polymorphism (snp) sites in different coding and non-coding regions and to analyse potent ... | 2015 | 25431006 |
| expression and localization of aquaporin-1 along the intestine of colostrum suckling buffalo calves. | aquaporin-1 (aqp1), a six-transmembrane domain protein, belongs to a highly conserved group of proteins called aquaporins known to regulate permeability across cell membranes. although the role of aqp1 has been extensively studied, its specific activity along the gastrointestinal tract in animals during early postnatal development is poorly known. this study investigates the expression of aqp1 mrna and protein in the small and large intestine of water buffalo calves after colostrum ingestion usi ... | 2015 | 25348329 |
| expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (bubalus bubalis) and the effect of insulin-like growth factor i on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro. | this study investigated the expression and localization of insulin-like growth factor (igf) system at different stages of buffalo cl and the role of igf-i in stimulating vascular endothelial growth factor (vegf) and progesterone (p4) production in cultured luteal cells. the mrna expression of igf system, vegf, steroidogenic acute regulatory protein, p450scc, and hydroxysteroid dehydrogenase (hsd) was investigated by quantitative real-time polymerase chain reaction (pcr). protein expression of ig ... | 2015 | 25304995 |
| effect of dietary protein on intake, nutrients utilization, nitrogen balance, blood metabolites, growth and puberty in growing bhadawari buffalo (bubalus bubalis) heifers. | fifteen bhadawari buffalo heifers of 207 ± 9.78 kg mean body weight were randomly distributed into three dietary groups to evaluate the effect of protein level on nutrient utilization, nitrogen (n) balance, growth rate, blood metabolites, and puberty. all animals were offered wheat straw-berseem diets supplemented with concentrate mixtures of similar energy (2.7 mcal/kg) and different protein levels (14.3-22%). animals of standard-protein group (spg) were offered protein and energy as per requir ... | 2015 | 25407740 |
| effect of additional chromium supplementation on health status, metabolic responses, and performance traits in periparturient murrah buffaloes (bubalus bubalis). | this study was conducted to determine the effects of inorganic chromium (cr) on body condition, metabolic responses, lactation performance, and reproductive parameters in periparturient murrah buffaloes. twenty-four multiparous advanced pregnant murrah buffaloes were randomly assigned to four treatment diets. feeding regimen was the same in all the groups, except that the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of cr/kg dry matter (dm) ... | 2015 | 25388756 |
| assessment of genetic variability and structuring of riverine buffalo population (bubalus bubalis) of indo-gangetic basin. | the buffalo population of uttar pradesh (up) constitutes 26.1% of the total buffalo population of india, yet this population has not been classified into distinct breeds or subpopulations due to lack of systematic study. genetic variation at 30 microsatellite loci was examined and statistical analysis was carried out to reveal genetic diversity, demographic parameters of these buffaloes and to investigate the existence of population substructures underlying geographical distribution. the mean nu ... | 2015 | 25380468 |
| expression of mrna encoding igf-i, igf-ii, type-i,and ii igf-receptors and igf-binding proteins-1-4 during ovarian follicular development in buffalo (bubalus bubalis). | the present study was designed to investigate the expression pattern of igf-i, igf-ii, type-i and ii igf-receptors, and igfbp-1-4 in different stages of buffalo ovarian preantral follicles (pfs), antral follicles (afs), ovulatory follicles (ofs), and immature (im) and in vitro matured (mo) oocytes. buffalo ovaries were collected from local abattoir, pfs (200-250 µm), afs (1-3 mm), and ofs (5-8 mm) were isolated by mechanical method. pfs, afs, ofs, and oocytes were lysed to release mrna, reverse ... | 2015 | 25380459 |
| morphologic and genotypic characterization of psoroptes mites from water buffaloes in egypt. | species delimitation of psoroptes spp. and identity of the parasite in water buffaloes remain poorly defined. in this study, psoroptes infestation on three water buffalo farms in egypt was examined based on morphometric characteristics, especially the opisthosomal setae of adult male mites. clinical investigations showed that 28% (196/700) of the sampled animals had mange infestation. microscopic examinations of 80 skin scrapings indicated the occurrence of psoroptes mites in 17 (21.3%) samples, ... | 2015 | 26517834 |