Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| role of emerging respiratory viruses in children with severe acute wheezing. | acute wheezing episodes are frequently associated with respiratory viral infections in children. however, the role of the recently described respiratory viruses is not yet fully understood. | 2010 | 20503284 |
| interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. | the predominance of severe respiratory syncytial virus (rsv) bronchiolitis in boys compared to girls is well known, but its mechanism is not yet understood. this is the first study focusing on gender-specific genetic factors affecting the risk of severe rsv infection using a previously described cohort. we determined 347 single-nucleotide polymorphisms (snps) in 470 children hospitalized for rsv infection, their parents, and 1,008 random population controls. we tested if these snps exerted a dif ... | 2010 | 20503287 |
| scientific discovery and scientific reputation: the reception of peyton rous' discovery of the chicken sarcoma virus. | this article concerns itself with the reception of rous' 1911 discovery of what later came to be known as the rous sarcoma virus (rsv). rous made his discovery at the rockefeller institute for medical research which had been primarily established to conduct research into infectious diseases. rous' chance discovery of a chicken tumor led him to a series of conjectures about cancer causation and about whether cancer could have an extrinsic cause. rous' finding was received with some scepticism by ... | 2010 | 20503720 |
| inhibition of ifn-gamma-dependent antiviral airway epithelial defense by cigarette smoke. | although individuals exposed to cigarette smoke are more susceptible to respiratory infection, the effects of cigarette smoke on lung defense are incompletely understood. because airway epithelial cell responses to type ii interferon (ifn) are critical in regulation of defense against many respiratory viral infections, we hypothesized that cigarette smoke has inhibitory effects on ifn-gamma-dependent antiviral mechanisms in epithelial cells in the airway. | 2010 | 20504369 |
| passive immunisation against respiratory syncytial virus: a cost-effectiveness analysis. | the cost-effectiveness of passive immunisation against respiratory syncytial virus (rsv) in the netherlands was studied by assessing incremental costs to prevent one hospitalisation in high-risk children using a novel individualised monthly approach. | 2010 | 20504841 |
| respiratory syncytial virus engineered to express the cystic fibrosis transmembrane conductance regulator corrects the bioelectric phenotype of human cystic fibrosis airway epithelium in vitro. | cystic fibrosis (cf) is the most common lethal recessive genetic disease in the caucasian population. it is caused by mutations in the cf transmembrane conductance regulator (cftr) gene that is normally expressed in ciliated airway epithelial cells and the submucosal glands of the lung. since the cftr gene was first characterized in 1989, a major goal has been to develop an effective gene therapy for cf lung disease, which has the potential to ameliorate morbidity and mortality. respiratory sync ... | 2010 | 20504917 |
| disease severity and viral load are correlated in infants with primary respiratory syncytial virus infection in the community. | respiratory syncytial virus (rsv) is a major cause of respiratory tract infections in infants, with remarkable variability in disease severity. factors determining severity of disease in previously healthy infants are still unclear. it was hypothesized that disease severity is correlated with viral load in primary rsv infection. infants of a healthy birth cohort were included at signs of their first respiratory tract infection. nasopharyngeal aspirate was obtained within 48-96 hr and disease sev ... | 2010 | 20513094 |
| viral respiratory infections in hospitalized and community control children in alaska. | respiratory syncytial virus (rsv) in alaska native children from the yukon kuskokwim (yk) delta is associated with a hospitalization rate five times higher than that reported for the general us child population. the role of other viral respiratory pathogens has not been studied in this population. yk delta children <3 years of age hospitalized with respiratory infections and same aged community control children were prospectively enrolled between october 2005 and september 2007. polymerase chain ... | 2010 | 20513097 |
| respiratory syncytial virus-neutralizing monoclonal antibodies motavizumab and palivizumab inhibit fusion. | respiratory syncytial virus (rsv) is a major cause of virus-induced respiratory disease and hospitalization in infants. palivizumab, an rsv-neutralizing monoclonal antibody, is used clinically to prevent serious rsv-related respiratory disease in high-risk infants. motavizumab, an affinity-optimized version of palivizumab, was developed to improve protection against rsv. these antibodies bind rsv f protein, which plays a role in virus attachment and mediates fusion. determining how these antibod ... | 2010 | 20519399 |
| respiratory syncytial virus limits alpha subunit of eukaryotic translation initiation factor 2 (eif2alpha) phosphorylation to maintain translation and viral replication. | the impact of respiratory syncytial virus (rsv) on morbidity and mortality is significant in that it causes bronchiolitis in infants, exacerbations in patients with obstructive lung disease, and pneumonia in immunocompromised hosts. rsv activates protein kinase r (pkr), a cellular kinase relevant to limiting viral replication (groskreutz, d. j., monick, m. m., powers, l. s., yarovinsky, t. o., look, d. c., and hunninghake, g. w. (2006) j. immunol. 176, 1733-1740). it is activated by autophosphor ... | 2010 | 20519500 |
| a highly attenuated recombinant human respiratory syncytial virus lacking the g protein induces long-lasting protection in cotton rats. | respiratory syncytial virus (rsv) is a primary cause of serious lower respiratory tract illness for which there is still no safe and effective vaccine available. using reverse genetics, recombinant (r)rsv and an rrsv lacking the g gene (deltag) were constructed based on a clinical rsv isolate (strain 98-25147-x). | 2010 | 20525213 |
| a phase 2, randomized, double-blind safety and pharmacokinetic assessment of respiratory syncytial virus (rsv) prophylaxis with motavizumab and palivizumab administered in the same season. | respiratory syncytial virus (rsv) is an important pathogen causing annual epidemics of bronchiolitis and pneumonia among infants worldwide. high-risk infants currently receive rsv prophylaxis with palivizumab, a humanized rsv monoclonal antibody (mab). in preclinical in vitro and in vivo (cotton-rat model) studies, motavizumab, a new rsv mab, was shown to have greater anti-rsv activity than palivizumab. motavizumab is currently under review for licensing approval. since both mabs may be availabl ... | 2010 | 20525274 |
| distinctive clinical features of human bocavirus in children younger than 2 years. | clinical characteristics of human bocavirus (hbov) infection have been studied worldwide, but their importance of those characteristics remains unknown. we investigated distinctive clinical features of hbov-positive children with lower respiratory tract infection (lrti). | 2010 | 20383526 |
| comparative costs of hospitalisation among infants at high risk for respiratory syncytial virus lower respiratory tract infection during the first year of life. | this retrospective cohort study compared the total cost of hospitalisation due to respiratory syncytial virus (rsv) lower respiratory tract infection (lri) during the first year of life between late-preterm (33-36 weeks gestational age [wga]) and term (≥ 37 wga) infants. | 2010 | 20128663 |
| interleukin 18 coexpression during respiratory syncytial virus infection results in enhanced disease mediated by natural killer cells. | respiratory syncytial virus (rsv) causes bronchiolitis, the main cause of infantile hospitalization. immunity against reinfection is poor, and there is great interest in boosting vaccine responses using live vectors expressing host cytokines. we therefore constructed a recombinant rsv expressing murine interleukin 18 (rsv/il-18), a cytokine capable of inducing strong antiviral immune responses. in vitro rsv/il-18 replicated at wild-type levels and produced soluble il-18. in naïve balb/c mice, rs ... | 2010 | 20130064 |
| a systematic review of compliance with palivizumab administration for rsv immunoprophylaxis. | respiratory syncytial virus (rsv) is a leading cause of lower respiratory tract infection (lrti) in infants and young children, accounting for approximately 75,000-125,000 hospitalizations per year. it is estimated that in 2000, rsv infection accounted for 1.7 million office visits, 402,000 emergency room visits, and 236,000 hospital outpatient visits per year for children younger than 5 years of age. palivizumab, a humanized monoclonal antibody directed against rsv, is the only immunoprophylaxi ... | 2010 | 20131495 |
| cost effectiveness of respiratory syncytial virus prophylaxis: a critical and systematic review. | respiratory syncytial virus (rsv) is the leading cause of infant hospitalization in the us. the economic burden of severe disease is substantial, including hospitalization costs and out-of-pocket expenses. rsv prophylaxis with either rsv immune globulin intravenous (rsv-igiv) or palivizumab has been shown to be effective in reducing rsv-related hospitalizations. motavizumab, a new enhanced-potency humanized rsv monoclonal antibody, is presently in clinical trials. rsv-igiv and palivizumab are as ... | 2010 | 20131925 |
| prevalence and clinical and molecular characterization of human metapneumovirus in children with acute respiratory infection in china. | human metapneumovirus (hmpv), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (artis). however, the prevalence and molecular characteristics of hmpv in china are still unclear. | 2010 | 20135829 |
| hospitalization for lower respiratory tract disease in preterm infants: effects of prophylaxis with palivizumab. | to evaluate the effect of palivizumab prophylaxis on hospitalization for acute respiratory tract infections (rti) in preterm infants, a prospective study was performed on a cohort of preterm infants [gestational age (ga) <32 weeks], admitted at birth to a neonatology intensive care unit (nicu) (follow-up: 30-month after discharge). 154 palivizumab-recipients and 71 palivizumab-non-recipients were evaluated. during follow-up, a similar rate of hospitalization for rti was found in the two groups ( ... | 2010 | 20227990 |
| proton-driven assembly of the rous sarcoma virus capsid protein results in the formation of icosahedral particles. | in a mature and infectious retroviral particle, the capsid protein (ca) forms a shell surrounding the genomic rna and the replicative machinery of the virus. the irregular nature of this capsid shell precludes direct atomic resolution structural analysis. ca hexamers and pentamers are the fundamental building blocks of the capsid, however the pentameric state, in particular, remains poorly characterized. we have developed an efficient in vitro protocol for studying the assembly of rous sarcoma v ... | 2010 | 20228062 |
| assessing the burden of paediatric influenza in europe: the european paediatric influenza analysis (epia) project. | the european paediatric influenza analysis (epia) project is a multi-country project that was created to collect, analyse and present data regarding the paediatric influenza burden in european countries, with the purpose of providing the necessary information to make evidence-based decisions regarding influenza immunisation recommendations for children. the initial approach taken is based on existing weekly virological and age-specific influenza-like illness (ili) data from surveillance networks ... | 2010 | 20229049 |
| methods for monitoring dynamics of pulmonary rsv replication by viral culture and by real-time reverse transcription-pcr in vivo: detection of abortive viral replication. | viral infection is normally detected either by viral culture or by pcr methods. rarely is a combination of the two techniques used in the same study. yet, when applied simultaneously, viral culture and pcr may reveal important features of viral biology, such as an abortive replication, as in the case of respiratory syncytial virus (rsv) infection. in this unit, we describe methods for detecting abortive rsv replication in a cotton rat model by using the plaque-forming unit assay and the real-tim ... | 2010 | 20235102 |
| pharmacologic advances in the treatment and prevention of respiratory syncytial virus. | currently, only 2 drugs have been approved for the treatment of respiratory syncytial virus (rsv). palivizumab is a monoclonal antibody for the prevention of rsv in high-risk children. ribavirin is approved for treatment of severe rsv disease; however, its effectiveness in improving outcomes is questionable. during the past 40 years, many obstacles have delayed the development of safe and effective vaccines and treatment regimens. this article reviews these obstacles and presents the novel devel ... | 2010 | 20235830 |
| validation of a model to predict hospitalization due to rsv of infants born at 33-35 weeks' gestation. | a model to predict hospitalization due to respiratory syncytial virus (rsv) of infants born at 33- 35 weeks' gestation was developed using seven risk factors from the spanish flip study "birth +/-10 weeks from the beginning of the rsv season", "birth weight", "breast fed <or=2 months", "number of siblings >or=2 years", "number of family members with atopy", "number of family members with wheezing", and "gender". the aim of this study was to validate the model using french data. | 2010 | 20297901 |
| respiratory syncytial virus outbreak defined by rapid screening in a neonatal intensive care unit. | palivizumab is currently licensed for the prevention of respiratory syncytial virus (rsv) lower respiratory tract disease in infants and children with chronic lung disease, with a history of preterm birth, or with haemodynamically significant congenital heart disease, but its routine use during outbreaks in neonatal intensive care units (nicus) is not currently recommended. here we report an outbreak in a nicu detected during a screening trial for rsv infection using a rapid antigen test (respi- ... | 2010 | 20299133 |
| detection and control of a nosocomial respiratory syncytial virus outbreak in a stem cell transplantation unit: the role of palivizumab. | respiratory syncytial virus (rsv) is a common community-acquired virus that causes upper and lower respiratory tract infections in children, hematologic malignancy patients, and hematopoietic stem cell transplant (hsct) recipients. nosocomial transmission of rsv in immunocompromised patients can significantly affect morbidity, mortality, and duration of hospitalization. stringent infection control measurements are needed to control further hospital transmission. prophylactic palivizumab was foun ... | 2010 | 20304082 |
| nosocomial transmission of respiratory syncytial virus in neonatal intensive care and intermediate care units: a prospective epidemiologic study. | to test the hypothesis that a considerable number of preterm infants acquire respiratory syncytial virus (rsv) within the hospital during the postnatal stay, a prospective epidemiologic survey was performed. nasopharyngeal swabs were taken twice weekly for a period of 8 weeks from preterm infants, medical/nursing staff, and parents during the peak of rsv season 2007/2008 and tested for rsv by polymerase chain reaction. of 1002 samples, only 4 tested positive (2 from a patient, 2 from staff). seq ... | 2010 | 20305582 |
| nmr relaxation studies of an rna-binding segment of the rous sarcoma virus gag polyprotein in free and bound states: a model for autoinhibition of assembly. | assembly of retrovirus particles is promoted by interaction of the gag polyprotein with rna. nonspecific rna association with the nucleocapsid domain (nc) of gag induces the dimerization of gag through protein-protein contacts in the capsid domain (ca), followed by higher order assembly to form the immature virus particle. nmr relaxation studies were conducted to investigate the initial steps of rous sarcoma virus (rsv) assembly by examining the association with nucleic acid of a fragment of gag ... | 2010 | 20387899 |
| an lypsl late domain in the gag protein contributes to the efficient release and replication of rous sarcoma virus. | the efficient release of newly assembled retrovirus particles from the plasma membrane requires the recruitment of a network of cellular proteins (escrt machinery) normally involved in the biogenesis of multivesicular bodies and in cytokinesis. retroviruses and other enveloped viruses recruit the escrt machinery through three classes of short amino acid consensus sequences termed late domains: pt/sap, ppxy, and lypx(n)l. the major late domain of rous sarcoma virus (rsv) has been mapped to a pppy ... | 2010 | 20392845 |
| heliox inhalation therapy for bronchiolitis in infants. | acute viral bronchiolitis is associated with airway obstruction and turbulent gas flow. heliox, a mixture of oxygen and the inert gas helium, may improve gas flow through high-resistance airways and decrease the work of breathing. | 2010 | 20393951 |
| children, parents and respiratory syncytial virus in palermo, italy: prevention is primary. | a study was conducted to describe the characteristics of the respiratory syncytial virus (rsv) infection cases occurring in the season 2006-7 in palermo, italy, and to evaluate the parents' knowledge and behaviours concerning prevention and control of acute respiratory infections (aris). all children aged between 0 and 2 years, admitted for a lower respiratory tract infection (lrti) between october 2006 and may 2007, were enrolled in the study. data were collected about demographic and household ... | 2010 | 20395316 |
| characterization of sirnas derived from rice stripe virus in infected rice plants by deep sequencing. | rna interference is a natural defense against viruses in plants. to date, the only viral sirnas characterized have been those for positive-sense rna viruses with one or two genome components. here, we characterized sirnas derived from rice stripe virus (rsv), a member of the genus tenuivirus with four genomic rnas and an ambisense coding strategy. deep sequencing of small rnas from infected rice leaves showed that sirnas were derived almost equally from virion and complementary rna strands and w ... | 2010 | 20396917 |
| global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. | the global burden of disease attributable to respiratory syncytial virus (rsv) remains unknown. we aimed to estimate the global incidence of and mortality from episodes of acute lower respiratory infection (alri) due to rsv in children younger than 5 years in 2005. | 2010 | 20399493 |
| autonomic dysfunction with early respiratory syncytial virus-related infection. | apparent life-threatening events (alte) and/or prolonged apnoea have been well-documented during respiratory syncytial virus (rsv) infection in infants less than 2 months of age but fundamental mechanisms remain unclear. the possibility of a central origin for the development of severe cardiac and respiratory events encouraged us, to explore the autonomic nervous system (ans) profile of infected infants, since ans activity may contribute to the constellation of symptoms observed during severe fo ... | 2010 | 20399711 |
| control of rsv-induced lung injury by alternatively activated macrophages is il-4r alpha-, tlr4-, and ifn-beta-dependent. | severe respiratory syncytial virus (rsv)-induced bronchiolitis has been associated with a mixed "th1" and "th2" cytokine storm. we hypothesized that differentiation of "alternatively activated" macrophages (aa-m phi) would mediate the resolution of rsv-induced lung injury. rsv induced interleukin (il)-4 and il-13 by murine lung and peritoneal macrophages, il-4r alpha/stat6-dependent aa-m phi differentiation, and significantly enhanced inflammation in the lungs of il-4r alpha(-/-) mice. adoptive ... | 2010 | 20404812 |
| self-inactivating alpharetroviral vectors with a split-packaging design. | accidental insertional activation of proto-oncogenes and potential vector mobilization pose serious challenges for human gene therapy using retroviral vectors. comparative analyses of integration sites of different retroviral vectors have elucidated distinct target site preferences, highlighting vectors based on the alpharetrovirus rous sarcoma virus (rsv) as those with the most neutral integration spectrum. to date, alpharetroviral vector systems are based mainly on single constructs containing ... | 2010 | 20410274 |
| respiratory syncytial virus-mediated nf-kappa b p65 phosphorylation at serine 536 is dependent on rig-i, traf6, and ikk beta. | respiratory syncytial virus (rsv) is the etiological agent of acute respiratory diseases, such as bronchiolitis and pneumonia. the exacerbated production of proinflammatory cytokines and chemokines in the airways in response to rsv is an important pillar in the development of these pathologies. as such, a keen understanding of the mechanisms that modulate the inflammatory response during rsv infection is of pivotal importance to developing effective treatment. the nf-kappab transcription factor ... | 2010 | 20410276 |
| emergency department septic screening in respiratory syncytial virus (rsv) and non-rsv bronchiolitis. | to identify factors associated with culture-proven serious bacterial infection (sbi) and positive emergency department septic screening (edss) tests in children with bronchiolitis and to identify factors associated with the performance of edss. | 2010 | 20411078 |
| narcissus tazetta lectin shows strong inhibitory effects against respiratory syncytial virus, influenza a (h1n1, h3n2, h5n1) and b viruses. | a mannose-binding lectin (narcissus tazetta lectin [ntl]) with potent antiviral activity was isolated and purified from the bulbs of the chinese daffodil narcissus tazetta var. chinensis, using ion exchange chromatography on diethylaminoethyl (deae)-cellulose, affinity chromatography on mannose-agarose and fast protein liquid chromatography (fplc)-gel filtration on superose 12. the purified lectin was shown to have an apparent molecular mass of 26 kda by gel filtration and 13 kda by sds-page, in ... | 2010 | 20413914 |
| functional disorder of primary immunity responding to respiratory syncytial virus infection in offspring mice exposed to a flame retardant, decabrominated diphenyl ether, perinatally. | perinatal exposure to a representative flame retardant, decabrominated diphenyl ether (dbde), was shown previously to increase viral titers in the lungs of respiratory syncytial virus (rsv)-infected offspring on day 5 post-infection, resulting in exacerbation of pneumonia. in this study, the significant increase of pulmonary viral titers was confirmed even on day 1 post-infection and the effect on the primary immune response to rsv infection were examined to assess a mode of dbde action on devel ... | 2010 | 20419825 |
| a randomized, double-blind, placebo-controlled study of an rnai-based therapy directed against respiratory syncytial virus. | rna interference (rnai) is a natural mechanism regulating protein expression that is mediated by small interfering rnas (sirna). harnessing rnai has potential to treat human disease; however, clinical evidence for the effectiveness of this therapeutic approach is lacking. aln-rsv01 is an sirna directed against the mrna of the respiratory syncytial virus (rsv) nucleocapsid (n) protein and has substantial antiviral activity in a murine model of rsv infection. we tested the antiviral activity of al ... | 2010 | 20421463 |
| [respiratory syncytial virus increases the expression and release of high mobility group box-1 protein in the lung tissue of mice]. | to investigate the expression and release of high mobility group box-1 protein (hmgb1) in the lung tissue of mice with respiratory syncytial virus (rsv) infection. | 2010 | 20423829 |
| [detection and clinical characterization of wu polyomavirus in acute respiratory tract infection in children]. | wu polyomavirus (wupyv), a new member of the genus polyomavirus in the family polyomaviridae, has been found to be associated with respiratory tract infections recently. but the role of the wupyv as agents of human disease remains uncertain. we sought to describe the detection and clinical characterization of wupyv in acute respiratory tract infection in children. | 2010 | 20426930 |
| [analysis of viral etiology of severe pneumonia in infants and young children in chongqing area]. | to investigate the prevalence of viral infections and putative association of viral infection with illness severity in young children with severe lower respiratory tract infection (lrti) in chongqing. | 2010 | 20426941 |
| a probasin promoter, conditionally replicating adenovirus that expresses the sodium iodide symporter (nis) for radiovirotherapy of prostate cancer. | the sodium iodide symporter (nis) directs the uptake and concentration of iodide in thyroid cells. we have extended the use of nis-mediated radioiodine therapy to other types of cancer, we transferred and expressed the nis gene into prostate, colon and breast cancer cells using adenoviral vectors. to improve vector efficiency we have developed a conditionally replicating adenovirus (crad) in which the e1a gene is driven by the prostate-specific promoter, probasin and the cassette rsv promoter hu ... | 2010 | 20428214 |
| causal direction between respiratory syncytial virus bronchiolitis and asthma studied in monozygotic twins. | respiratory syncytial virus (rsv) bronchiolitis has been associated with later development of asthma, wheezing, abnormal pulmonary function, and sensitization. our aim was to determine the differential effect within monozygotic (mz) twin pairs discordant for severe rsv bronchiolitis in infancy on the subsequent development of asthma, pulmonary function, and allergy. | 2010 | 20435661 |
| directionality of nucleocytoplasmic transport of the retroviral gag protein depends on sequential binding of karyopherins and viral rna. | retroviral gag polyproteins coopt host factors to traffic from cytosolic ribosomes to the plasma membrane, where virions are released. before membrane transport, the multidomain gag protein of rous sarcoma virus (rsv) undergoes importin-mediated nuclear import and crm1-dependent nuclear export, an intrinsic step in the assembly pathway. transient nuclear trafficking of gag is required for efficient viral rna (vrna) encapsidation, suggesting that gag:vrna binding might occur in the nucleus. here, ... | 2010 | 20435918 |
| montelukast during primary infection prevents airway hyperresponsiveness and inflammation after reinfection with respiratory syncytial virus. | respiratory syncytial virus (rsv) bronchiolitis in infants may be followed by the development of asthma-like symptoms. age at first infection dictates consequences upon reinfection. reinfection of mice initially exposed as neonates to rsv enhanced development of airway hyperresponsiveness (ahr), eosinophilic inflammation, and mucus hyperproduction. rsv lower respiratory tract disease is associated with activation of the leukotriene pathway. | 2010 | 20442434 |
| development of multiplex nucleic acid sequence-based amplification for detection of human respiratory tract viruses. | a group of common lower respiratory tract infections, influenza a, influenza b, human parainfluenza virus 1-4 (hpiv1-4), respiratory syncytial virus (rsv), rubella virus (rv) and coxsackie virus (csv), were selected for the development of a multiplex nucleic acid sequence-based amplification (nasba) assay. quantifiable measurement utilizing an enzyme-linked oligonucleotide capture (eoc) optical detection method, which was described previously, alleviated the requirement of specialized instrument ... | 2010 | 20447419 |
| respiratory syncytial virus infection outbreak among pediatric patients with oncologic diseases and/or bmt. | respiratory syncytial virus (rsv) has been reported to cause severe morbidity and mortality among cancer patients receiving chemotherapy with or without autologous/allogeneic hematopoetic stem cell transplantation (hsct). there have been few reports describing the outcome of rsv infection specifically among pediatric oncology patients. | 2010 | 20146398 |
| genetic variability of respiratory syncytial viruses (rsv) prevalent in southwestern china from 2006 to 2009: emergence of subgroup b and a rsv as dominant strains. | respiratory syncytial virus (rsv) is the most commonly identified viral agent in young children with acute respiratory tract infections (aris) and often causes repeated infections throughout life. this study investigated the genetic variability of the attachment (g) protein gene among rsv strains prevalent in southwestern china. reverse transcription-pcr (rt-pcr) for a fragment of the rsv g gene was performed with nasopharyngeal aspirates (npas) collected from children with aris hospitalized in ... | 2010 | 20147636 |
| cellular processes of v-src transformation revealed by gene profiling of primary cells--implications for human cancer. | cell transformation by the src tyrosine kinase is characterized by extensive changes in gene expression. in this study, we took advantage of several strains of the rous sarcoma virus (rsv) to characterize the patterns of v-src-dependent gene expression in two different primary cell types, namely chicken embryo fibroblasts (cef) and chicken neuroretinal (cnr) cells. we identified a common set of v-src regulated genes and assessed if their expression is associated with disease-free survival using ... | 2010 | 20152043 |
| the effect of grafted methoxypoly(ethylene glycol) chain length on the inhibition of respiratory syncytial virus (rsv) infection and proliferation. | respiratory syncytial virus (rsv) is a significant cause of morbidity in humans. to date, no effective treatments exist and current prophylactic therapy access is limited and is only approximately 50% effective. to attenuate the risk of rsv infection, we hypothesized that bioengineering of either the virus particle or host cell via the covalent grafting of methoxypoly(ethylene glycol) [mpeg] would prevent infection. to this end, the anti-viral effects of grafting concentration, linker chemistry ... | 2010 | 20153523 |
| an overview on different classes of viral entry and respiratory syncitial virus (rsv) fusion inhibitors. | the therapeutic approach to aids is based on the combination of different drugs in the highly active antiretroviral therapy (haart) regimen. these drugs have a wide variety of side effects, and some strains of hiv can develop resistance: for these reasons new anti-hiv drugs are needed. in the wide field of anti-hiv medicine this review covers different classes of drugs which inhibit viral entry: in particular the classification of main categories, their mode of action and some new candidates for ... | 2010 | 20156158 |
| the challenges of rsv vaccines. where do we stand? | the most realistic way to control rsv infection would be the development of an effective and safe vaccine. a formalin-inactivated rsv vaccine was evaluated in infants and children in the 1960's which disappointingly was linked with aggravation of rsv disease following the natural infection. two candidate vaccines with purified protein f, have been tested, in humans and have been considered safe and somewhat immunogenic in seropositive persons providing different levels of protection against rsv ... | 2010 | 20156180 |
| impact of the 2009 influenza a(h1n1) pandemic wave on the pattern of hibernal respiratory virus epidemics, france, 2009. | this short report based on clinical surveillance and laboratory data describes the circulation of rhinoviruses, influenza viruses and respiratory syncytial viruses (rsv) in france during the 2009-10 season compared with the previous winter season. the delayed circulation of rsv observed in 2009-10 compared with 2008-09 suggests that the early circulation of the 2009 pandemic influenza a(h1n1) viruses had an impact on the rsv epidemic. | 2010 | 20158981 |
| detection of new respiratory viruses in hospitalized infants with bronchiolitis: a three-year prospective study. | we have designed a study with the objective of describing the clinical impact of other viruses different from the respiratory syncytial virus (rsv) in hospitalized infants with bronchiolitis. | 2010 | 20163373 |
| identification of and human serum reactogenicity to neutralizing epitopes within the central unglycosylated region of the respiratory syncytial virus attachment protein. | we identified two overlapping neutralizing epitopes within residues 151 to 172 of the central unglycosylated region of the respiratory syncytial virus (rsv) attachment protein. in approximately 40% of hospitalized and outpatient adults infected with rsv subtype a, these contiguous residues are the target of > or =4-fold increases in igg response between acute- and convalescent-phase sera. | 2010 | 20164253 |
| palivizumab for prophylaxis against respiratory syncytial virus infection in children with cystic fibrosis. | respiratory syncytial virus (rsv) infection causes acute lung infection in infants and young children worldwide, resulting in considerable morbidity and mortality. children with cystic fibrosis (cf) are prone to recurrent lung inflammation, bacterial colonisation and subsequent chronic airway disease, putting them at risk for severe rsv infections requiring intensive care and respiratory support. no treatment currently exists, hence prevention is important. palivizumab is effective in reducing r ... | 2010 | 20166098 |
| [analysis of clinical features of community-acquired pneumonia caused by pediatric respiratory syncytial virus and human metapneumovirus]. | we retrospectively reviewed the background, clinical features, blood tests, and complications in the 720 children seen for acute respiratory tract infection from july 2004 to december 2005. of these, 75 (10.5%) were diagnosed with pneumonia due to respiratory syncytial virus (rsv) and 19 (2.6%) with pneumonia due to human metapneumovirus (hmpv) based on multiplex pcr analysis of nasopharyngeal samples. rsv was pcr-positive mostly in winter, -from november to january-, and hmpv mostly in spring, ... | 2010 | 20170013 |
| a randomized intervention of montelukast for post-bronchiolitis: effect on eosinophil degranulation. | to investigate the effect of montelukast on eosinophil degranulation and recurrent wheezing episodes in post-respiratory syncytial virus (rsv) bronchiolitis. | 2010 | 20171653 |
| respiratory syncytial virus infection reduces lung inflammation and fibrosis in mice exposed to vanadium pentoxide. | vanadium pentoxide (v2o5) exposure is a cause of occupational bronchitis and airway fibrosis. respiratory syncytial virus (rsv) is a ubiquitous pathogen that causes airway inflammation. it is unknown whether individuals with pre-existing respiratory viral infection are susceptible to v2o5-induced bronchitis. we hypothesized that respiratory viral infection will exacerbate vanadium-induced lung fibrosis. | 2010 | 20175905 |
| susceptibility to bronchiolitis in infants. | bronchiolitis is a complex disease that exhibits tremendous heterogeneity with respect to cause, clinical presentation, outcome and susceptibility of afflicted patients. although respiratory syncytial virus (rsv) is widely considered to be the most important cause of bronchiolitis in children, little is known about the mechanisms of susceptibility to severe infection. | 2010 | 20186062 |
| clinical and epidemiological comparison of human metapneumovirus and respiratory syncytial virus in seoul, korea, 2003-2008. | human metapneumovirus (hmpv) shares clinical and epidemiological characteristics with well-known respiratory syncytial virus (rsv). the aim of this study was to investigate the clinical and epidemiological differences between hmpv- and rsv-induced wheezing illnesses. a total of 1,008 nasopharyngeal aspirate specimens was collected from 1,008 pediatric patients hospitalized with acute respiratory tract infection at inje university sanggye paik hospital from december 2003 to april 2008, and tested ... | 2010 | 20191030 |
| the chemokine mip1alpha/ccl3 determines pathology in primary rsv infection by regulating the balance of t cell populations in the murine lung. | cd8 t cells assist in the clearance of respiratory syncytial virus (rsv) infection from the lungs. however, disease after rsv infection is in part caused by excessive t cell activity, and a balance is therefore needed between beneficial and harmful cellular immune responses. the chemokine ccl3 (mip1alpha) is produced following rsv infection and is broadly chemotactic for both t cells and natural killer (nk) cells. we therefore investigated its role in rsv disease. | 2010 | 20195359 |
| delivery of cytokines by recombinant virus in early life alters the immune response to adult lung infection. | respiratory syncytial virus (rsv) is the main cause of bronchiolitis, the major cause of hospitalization of infants. an ideal rsv vaccine would be effective for neonates, but the immune responses of infants differ markedly from those of adults, often showing a bias toward t-helper 2 (th2) responses and reduced gamma interferon (ifn-gamma) production. we previously developed recombinant rsv vectors expressing ifn-gamma and interleukin-4 (il-4) that allow us to explore the role of these key th1 an ... | 2010 | 20200251 |
| nanoparticle detection of respiratory infection. | respiratory viruses are a constant concern for all demographics. examples include established viruses such as respiratory syncytial virus (rsv), the leading cause of respiratory infection in infants and young children, and emerging viruses such as severe acute respiratory syndrome (sars), which reached near pandemic levels in 2003, or h1n1 (swine) influenza. despite this prevalence, traditional methods of virus detection are typically labor intensive and require several days to successfully conf ... | 2010 | 20201109 |
| respiratory syncytial virus activity - united states, july 2008-december 2009. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis and pneumonia in children aged <1 year worldwide. each year in the united states, an estimated 75,000-125,000 infants are hospitalized with rsv. among adults aged >65 years, an estimated 177,000 hospitalizations and 14,000 deaths a year have been attributed to rsv infections. in temperate climates, the rsv season generally begins during the fall and continues through the winter and spring, but the exact timing of rsv cir ... | 2010 | 20203556 |
| respiratory syncytial virus f and g proteins induce interleukin 1alpha, cc, and cxc chemokine responses by normal human bronchoepithelial cells. | human respiratory syncytial virus (rsv) is a ubiquitous respiratory virus that causes serious lower respiratory tract disease in infants and young children worldwide. studies have shown that rsv infection modulates chemokine expression patterns, suggesting that particular cytokine expression profiles may be indicators of disease severity. in this study, we show that rsv f or g protein treatment of fully differentiated primary normal human bronchial epithelial cells induces apical and basolateral ... | 2010 | 20205592 |
| differential expression of cytokine transcripts in neonatal and adult ovine alveolar macrophages in response to respiratory syncytial virus or toll-like receptor ligation. | alveolar macrophages (amvarphis) secrete regulatory molecules that are believed to be critical in maintaining normal lung homeostasis. however, in response to activating signals, amvarphis have been shown to become highly phagocytic cells capable of secreting significant levels of pro-inflammatory cytokines. there is evidence to suggest that susceptibility of mvarphi subpopulations to viral infection, and their subsequent cytokine/chemokine response, is dependent on age of the host. in the prese ... | 2010 | 20207014 |
| correlation between inflammatory mediators in the nasopharyngeal secretion and in the serum of children with lower respiratory tract infection caused by respiratory syncytial virus and disease severity. | to determine whether the concentrations of inflammatory mediators (ccl5, soluble intercellular adhesion molecule type 1 [sicam-1], tnf-alpha, il-6 and il-10) in the nasopharyngeal secretion and in the serum of children with lower respiratory tract infection (lrti) caused by respiratory syncytial virus (rsv) correlate with the clinical markers of disease severity. | 2010 | 20209309 |
| transgenic apple expressing an antigenic protein of the human respiratory syncytial virus. | a gene coding for the human respiratory syncytial virus (rsv)-f protein, driven by the constitutively expressed camv 35s promoter, was introduced into leaf tissues of apple, malusxdomestica borkh. cv. royal gala, via agrobacterium-mediated transformation. two putative transgenic lines were identified, and the presence of the rsv-f gene was confirmed by polymerase chain reaction (pcr). a total of 25 plants from these different transgenic events were successfully rooted, acclimatized, and transfer ... | 2010 | 20307914 |
| genotypes of chicken major histocompatibility complex b locus associated with regression of rous sarcoma virus j-strain tumors. | the chicken mhc-b locus affects the response to several strains of rous sarcoma virus (rsv). we evaluated the association between haplotypes of the mhc-b locus and responses to the j strain of rsv by using an f(2) experimental resource family constructed with tumor-regressive (white leghorn) and tumor-progressive (rhode island red) chickens. the mhc-b haplotypes were determined by genotyping of the microsatellite marker lei0258 and mhc-b locus class i alpha chain 2 (bf2). two haplotypes in the r ... | 2010 | 20308396 |
| [effects of different factors on the expression of thymic stromal lymphopoietin in respiratory syncytial virus-infected human airway epithelial cells]. | to investigate the effects of different factors on the expressions of thymic stromal lymphopoietin (tslp) in respiratory syncytial virus (rsv)-infected human airway epithelial cell line 16hbe cells. | 2010 | 20335125 |
| molecular detection and genetic variability of human metapneumovirus in uruguay. | human metapneumovirus (hmpv) has been described as circulating among the uruguayan population at least since 1998 based on serologic evidence. however, no isolation attempts, molecular detection, or genetic studies have been carried out so far in the country. in the present study, molecular detection of circulating hmpv in children hospitalized with acute respiratory tract infection in montevideo-uruguay was carried out by reverse transcription-polymerase chain reaction (rt-pcr) amplification of ... | 2010 | 20336730 |
| respiratory virus pneumonia after hematopoietic cell transplantation (hct): associations between viral load in bronchoalveolar lavage samples, viral rna detection in serum samples, and clinical outcomes of hct. | few data exist on respiratory virus quantitation in lower respiratory samples and detection in serum from hematopoietic cell transplant (hct) recipients with respiratory virus-associated pneumonia. | 2010 | 20350162 |
| [microbiological etiology in children with community acquired pneumonia]. | to study the distribution of pathogenic microorganisms of community acquired pneumonia (cap) in children. | 2010 | 20350426 |
| a prime-boost vaccination strategy using attenuated salmonella typhimurium and a replication-deficient recombinant adenovirus vector elicits protective immunity against human respiratory syncytial virus. | human respiratory syncytial virus (rsv), for which no clinically approved vaccine is available yet, is globally a serious pediatric pathogen of the lower respiratory tract. several approaches have been used to develop vaccines against rsv, but none of these have been approved for use in humans. an efficient vaccine-enhancing strategy for rsv is still urgently needed. we found previously that oral sl7207/pcdna3.1/f and intranasal fgad/f were able to induce an effective protective immune response ... | 2010 | 20350532 |
| respiratory viruses in nepalese children with and without pneumonia: a case-control study. | the causative role of respiratory viruses detected in upper airway secretions in childhood pneumonia needs further investigation. | 2010 | 20351618 |
| prospects for defined epitope vaccines for respiratory syncytial virus. | the history of vaccines for respiratory syncytial virus (rsv) illustrates the complex immunity and immunopathology to this ubiquitous virus, starting from the failed formalin-inactivated vaccine trials performed in the 1960s. an attractive alternative to traditional live or killed virus vaccines is a defined vaccine composed of discrete antigenic epitopes for which immunological activities have been characterized as comprehensively as possible. here we present cumulative data on murine and human ... | 2010 | 20353300 |
| the epidemiology of hospitalized influenza in children, a two year population-based study in the people's republic of china. | the epidemiology and disease burden of annual influenza in children in mainland people's republic of china have not been reported in detail. to understand the incidence and epidemiology of laboratory-proven influenza hospitalization in children in china, a review of available laboratory and hospital admission data was undertaken. | 2010 | 20353557 |
| incidence of respiratory pathogens in persons hospitalized with pneumonia in two provinces in thailand. | although pneumonia is a leading cause of death from infectious disease worldwide, comprehensive information about its causes and incidence in low- and middle-income countries is lacking. active surveillance of hospitalized patients with pneumonia is ongoing in thailand. consenting patients are tested for seven bacterial and 14 viral respiratory pathogens by pcr and viral culture on nasopharyngeal swab specimens, serology on acute/convalescent sera, sputum smears and antigen detection tests on ur ... | 2010 | 20353622 |
| antiviral activity of benzimidazole derivatives. ii. antiviral activity of 2-phenylbenzimidazole derivatives. | seventy-six 2-phenylbenzimidazole derivatives were synthesized and evaluated in cell-based assays for cytotoxicity and antiviral activity against a panel of 10 rna and dna viruses. the most commonly affected viruses were, in decreasing order, cvb-2, bvdv, sb-1, hsv-1, and yfv, while hiv-1 and vsv were not affected, and rsv, vv and reo-1 were only susceptible to a few compounds. thirty-nine compounds exhibited high activity (ec(50)=0.1-10microm) against at least one virus, and four of them were o ... | 2010 | 20359898 |
| development of a multiplex real-time rt-pcr assay for detection of influenza a, influenza b, rsv and typing of the 2009-h1n1 influenza virus. | a high-throughput real-time rt-pcr assay was developed to amplify and detect a conserved region of the hemagglutinin gene of the 2009-h1n1 influenza a virus using a minor groove binder-conjugated hybridization probe. the assay was paired with a separate triplex real-time assay that detects influenza a via the matrix gene, influenza b and rsv in a multiplex format and compared with the centers for disease control and prevention (cdc) rrt-pcr assay using 143 samples. the 2009-h1n1 portion of the m ... | 2010 | 20362006 |
| single mucosal immunization of recombinant adenovirus-based vaccine expressing f1 protein fragment induces protective mucosal immunity against respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a major cause of severe lower respiratory tract disease in infancy and early childhood. despite its importance as a pathogen, there is no licensed vaccine against rsv. the fusion (f) protein of rsv is a potentially important target for protective antiviral immune responses. here, we studied the immune responses elicited by recombinant replication-deficient adenovirus (rad)-based vaccines expressing the soluble f1 fragment of f protein (amino acids 155-524) in ... | 2010 | 20362203 |
| etiology of bronchiolitis in a hospitalized pediatric population: prospective multicenter study. | in 2006, bronchiolitis due to adenovirus nosocomial infections resulted in the closure of a pediatric department in northern portugal. | 2010 | 20362492 |
| response network analysis of differential gene expression in human epithelial lung cells during avian influenza infections. | the recent emergence of the h5n1 influenza virus from avian reservoirs has raised concern about future influenza strains of high virulence emerging that could easily infect humans. we analyzed differential gene expression of lung epithelial cells to compare the response to h5n1 infection with a more benign infection with respiratory syncytial virus (rsv). these gene expression data are then used as seeds to find important nodes by using a novel combination of the gene ontology database and the h ... | 2010 | 20370926 |
| antiviral activity of daphnoretin isolated from wikstroemia indica. | the ethanol extract of wikstroemia indica was fractionated with organic solvents of different polarities, and various fractions were screened for their antiviral activity against respiratory syncytial virus (rsv) using a cytopathic effect (cpe) reduction assay. the ethyl acetate fraction was most active against rsv with 50% inhibition concentration (ic(50)) value < 3.9 microg/ml and a selectivity index (si) > 64.1. further isolation and purification of the fraction led to a purified compound, da ... | 2010 | 19610034 |
| incidence of common respiratory viral infections related to climate factors in hospitalized children in hong kong. | hong kong has a subtropical climate and an influenza seasonality lying approximately mid-way (march-june) between those of the northern (november-march) and southern (june-september) hemispheres. respiratory syncytial virus (rsv) shares a similar seasonality to that of influenza in hong kong and is another important respiratory infection of childhood. daily virus incidence data from public hospitals in hong kong's new territory east cluster, together with hong kong climate data were obtained for ... | 2010 | 19631018 |
| potential role of soluble trail in epithelial injury in children with severe rsv infection. | lower respiratory tract infection by respiratory syncytial virus (rsv) is a frequent cause of acute lung injury in young children and infants. studies in adults and animals suggest that tumor necrosis factor receptor (tnfr) ligands may mediate lung injury by causing apoptosis of epithelial cells. the main goal of the present study was to determine whether the tnf-related apoptosis-inducing ligand (apo2l/trail) pathway may be implicated in epithelial injury during severe rsv infection in children ... | 2010 | 19635930 |
| prevalence of respiratory syncytial virus (rsv) risk factors and cost implications of immunoprophylaxis to infants 32 to 35 weeks gestation for health plans in the united states. | during the period of this study, the american academy of pediatrics (aap) 2006 guidelines recommended respiratory syncytial virus (rsv) prophylaxis for infants 32 to 35 weeks gestation age (wga) with two or more of five risk factors (rfs). new recommendations have recently been published in 2009. the cost implications of expanding this list of rfs to include other evidence-based rfs like passive smoke exposure (pse), crowded living conditions (clcs), and young chronological age (yca) are unclear ... | 2010 | 19706010 |
| survey of severe respiratory syncytial virus infection in kyoto prefecture from 2003 to 2007. | respiratory syncytial virus (rsv) infection in infants can develop into a severe condition. | 2010 | 19761516 |
| a multi-drug regimen for respiratory syncytial virus and parainfluenza virus infections in adult lung and heart-lung transplant recipients. | respiratory syncytial virus (rsv) and parainfluenza virus (piv) can cause significant morbidity and mortality in lung and heart-lung transplant recipients. we evaluated the utility of a multi-drug protocol for the treatment of rsv- and piv-related infections. | 2010 | 19761558 |
| association of human metapneumovirus with radiologically diagnosed community-acquired alveolar pneumonia in young children. | to determine the involvement of human metapneumovirus (hmpv) in childhood community-acquired alveolar pneumonia (caap) and compare the demographic, clinical, and laboratory features of hmpv-associated caap and caap associated with other respiratory viruses. | 2010 | 19782998 |
| respiratory syncytial virus infection and palivizumab: are families receiving accurate information? | the aim of this study was to assess the accuracy of information provided by medical practitioners to families regarding the efficacy and limitations of prophylaxis with palivizumab for respiratory syncytial virus (rsv) infection in infants. a single-question survey was distributed to medical practitioners who described themselves as having both prescribed palivizumab for infants and discussed the effects of the drug with families. responses were anonymous and returned immediately after reviewing ... | 2010 | 19784911 |
| optimized adeno-associated virus (aav)-protein phosphatase-5 helper viruses for efficient liver transduction by single-stranded aav vectors: therapeutic expression of factor ix at reduced vector doses. | abstract our studies have shown that coinjection of conventional single-stranded adeno-associated virus 2 (ssaav2) vectors carrying the enhanced green fluorescent protein (egfp) gene with self-complementary (sc) aav2-t cell protein tyrosine phosphatase (tc-ptp) and scaav2-protein phosphatase-5 (pp5) vectors resulted in an approximately 16-fold increase in egfp expression in primary murine hepatocytes in vivo [jayandharan, g.r., zhong, l., li, b., kachniarz, b., and srivastava, a. (2008). gene th ... | 2010 | 19788390 |
| human hepg2 cells support respiratory syncytial virus and human metapneumovirus replication. | human metapneumovirus (hmpv) and human respiratory syncytial (rsv) virus cause mild to severe infections of the respiratory tract in all age groups. so far, several cell lines derived from respiratory tissues have been identified to support replication of both viruses. unfortunately, titers attained during replication differ between the both viruses within one cell line despite equal infection conditions, on the one hand giving raise to the assumption that the individual susceptibility may vary ... | 2010 | 19799934 |
| selective modification of rice (oryza sativa) gene expression by rice stripe virus infection. | rice stripe disease, caused by rice stripe virus (rsv), is one of the major virus diseases in east asia. rice plants infected with rsv usually show symptoms such as chlorosis, weakness, necrosis in newly emerged leaves and stunting. to reveal rice cellular systems influenced by rsv infection, temporal changes in the transcriptome of rsv-infected plants were monitored by a customized rice oligoarray system. the transcriptome changes in rsv-infected plants indicated that protein-synthesis machiner ... | 2010 | 19793907 |
| respiratory syncytial virus, human bocavirus and rhinovirus bronchiolitis in infants. | to investigate the prevalence of 14 viruses in infants with bronchiolitis and to study demographic and clinical differences in those with respiratory syncytial virus (rsv), human bocavirus (hbov) and rhinovirus (rv) infection. | 2010 | 19822538 |
| low incidence of severe respiratory syncytial virus infections in lung transplant recipients despite the absence of specific therapy. | respiratory syncytial virus (rsv) infections in lung transplant recipients (ltrs) have been associated with significant morbidity and mortality. immunoglobulins, ribavirin, and palivizumab are suggested treatments for both pre-emptive and therapeutic purposes. however, in the absence of randomized, placebo-controlled trials, efficacy is controversial and there is toxicity as well as cost concerns. | 2010 | 19837611 |