Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| analyses of the mrna transcription processes of snowshoe hare bunyavirus s and m rna species. | the time course of synthesis of snowshoe hare bunyavirus small (s)- and medium (m)-sized viral rna (vrna), viral crna (vcrna), and mrna species was analyzed by using single-stranded dna probes representing the s- and m-coded gene products. in the presence of puromycin, an inhibitor of protein synthesis, the subgenomic s mrna species were detected, but not full-length s vcrna or s vrna species. no m-related rna species were identified in puromycin-treated cells. in the absence of puromycin, full- ... | 1985 | 4020962 |
| effect of monensin on the assembly of uukuniemi virus in the golgi complex. | the effect of the carboxylic ionophore monensin on the maturation of uukuniemi virus, a bunyavirus, and the transport of its two membrane glycoproteins, g1 and g2, were studied in chicken embryo fibroblasts and baby hamster kidney cells. virus maturation, which occurs in the golgi complex (e. kuismanen, k. hedman, j. saraste, and r. f. pettersson, mol. cell. biol. 2:1444-1458, 1982; e. kuismanen, b. bång, m. hurme, and r. f. pettersson, j. virol. 51:137-146, 1984), was effectively inhibited by t ... | 1985 | 4020969 |
| evolution of bunyaviruses by genome reassortment in dually infected mosquitoes (aedes triseriatus). | aedes triseriatus mosquitoes became dually infected after ingesting two mutants of lacrosse (lac) virus simultaneously or after ingesting, by interrupted feeding, the two viruses sequentially within a 2-day period. after 2 weeks of incubation, approximately 25 percent of the vectors contained new virus genotypes as the result of rna segment reassortment. new viruses were transmitted when the mosquitoes fed on mice. viruses ingested more than 2 days after the initial infecting virus did not cause ... | 1985 | 4048949 |
| identification of nonstructural proteins encoded by viruses of the bunyamwera serogroup (family bunyaviridae). | the proteins synthesized in bhk cells infected with nine members of the bunyamwera serogroup (family bunyaviridae, bunyavirus genus) were analyzed by polyacrylamide gel electrophoresis. in addition to the virus structural proteins, a number of virus-coded nonstructural proteins were detected. one protein, designated ns1, was shown to be related to the nucleocapsid protein by one-dimensional peptide mapping. a second protein, ns2, was mapped to the m rna segment by gel electrophoretic analysis of ... | 1985 | 4060579 |
| the isolation of arboviruses including a new flavivirus and a new bunyavirus from ixodes (ceratixodes) uriae (ixodoidea: ixodidae) collected at macquarie island, australia, 1975-1979. | pools of ticks, ixodes (ceratixodes) uriae collected between 1975 and 1979 at macquarie island, yielded 33 strains of at least 4 different viruses: nugget virus (kemerovo group), 1 strain; taggert virus (sakhalin group) 9 strains; a previously undescribed flavivirus, related to central european tickborne encephalitis virus, for which the name "gadgets gully" is proposed, 9 strains; a virus serologically related to the uukuniemi serogroup, family bunyaviridae, for which the name "precarious point ... | 1985 | 2984951 |
| [isolation of the tahyña bunyavirus in the arctic]. | tahyna virus (bunyaviridae, bunyavirus, the california encephalitis complex) was isolated from aedes communis complex mosquitoes collected at the border of the north-taiga landscape zone (in latitude 68 degrees north and longitude 33 degrees east) at the kolsky peninsula (the murmansk region). the leiv-9843 mur strain was isolated from 2.4 thousand mosquitoes collected there (altogether 3.8 thousand mosquitoes had been collected in the murmansk region). this is the first isolation in the ussr of ... | 1985 | 2937203 |
| arbovirus investigations in argentina, 1977-1980. iii. identification and characterization of viruses isolated, including new subtypes of western and venezuelan equine encephalitis viruses and four new bunyaviruses (las maloyas, resistencia, barranqueras, and antequera). | forty viruses isolated from mosquitoes between 1977 and 1980 in argentina have been identified and characterized. nineteen strains of vee virus, identical by neutralization (n) tests, were shown by hemagglutination-inhibition tests with anti-e2 glycoprotein sera to represent a new subtype vi of the vee complex. rna oligonucleotide fingerprints of this virus were distinct from subtype i viruses. the virus was not lethal for english short-haired guinea pigs, indicating that it is probably not equi ... | 1985 | 2863990 |
| synthesis of tacaribe virus polypeptides in an in vitro coupled transcription and translation system. | we have analyzed polypeptides synthesized in a coupled in vitro transcription and translation system in response to detergent-disrupted tacaribe virus. analysis of the major tacaribe virus-specified product by two-dimensional polyacrylamide gel electrophoresis indicated that it had an isoelectric point similar to that of the tacaribe nucleocapsid polypeptide n; however, the in vitro product had an approximate mol. wt. of 73 000, compared to a mol. wt. of 68 000 for the n protein. the 73 000 dalt ... | 1985 | 2408399 |
| epitopes of the g1 glycoprotein of la crosse virus form overlapping clusters within a single antigenic site. | antigenic sites on the g1 glycoprotein of la crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (f. gonzalez-scarano, r. e. shope, c. h. calisher, and n. nathanson (1982), virology 120, 42-53). to analyze the relationship between the individual epitopes delineated by monoclonal antibodies, 11 neutralizing antibodies were used to select variant viruses. these variant viruses were tested against the panel of anti-g1 protein monoclonal a ... | 1985 | 2414920 |
| analyses of the mrna transcription processes of punta toro phlebovirus (bunyaviridae). | the time course of the syntheses of punta toro (pt) phlebovirus (bunyaviridae) small (s)-size viral rna (s vrna), viral complementary rna (s vcrna), and messenger rna (s mrna) species has been analyzed using single-stranded dna probes representing the two s-coded gene products. the data obtained support the conclusion that pt s rna has an ambisense coding strategy (t. ihara, h. akashi, and d. h. l. bishop, virology 136, 293-306, 1984) with the viral nucleocapsid protein, n, encoded in a viral-co ... | 1985 | 2416115 |
| an avirulent g1 glycoprotein variant of la crosse bunyavirus with defective fusion function. | la crosse virus, a member of the california serogroup of the family bunyaviridae, causes encephalitis in humans and laboratory rodents. a variant virus (v22) selected with a monoclonal antibody against the large (g1) glycoprotein showed diminished neuroinvasiveness after peripheral inoculation. this variant has an alteration in its fusion function, requiring a lower ph for the activation of fusion and demonstrating reduced efficiency of cell-to-cell fusion of bhk-21 cultures. v22 was studied in ... | 1985 | 3889368 |
| bunyavirus pathogenesis. | 1985 | 3938613 | |
| the complete sequence of the m rna of snowshoe hare bunyavirus reveals the presence of internal hydrophobic domains in the viral glycoprotein. | the complete sequence of the viral m rna of snowshoe hare (ssh) bunyavirus has been determined. the rna is 4527 nucleotides long (mol wt: 1.5 x 10(6), base composition: 27.5% a, 33.5% u, 17.7% g, 21.3% c), and has 3' and 5' terminal sequences that, depending on how they are arranged, are complementary for some 44 residues. the viral rna codes in its viral-complementary sequence, for a single primary gene product (the viral glycoprotein) that is comprised of 1441 amino acids (162,391 da), and is ... | 1984 | 6091326 |
| synergistic antiviral effects of ribavirin and the c-nucleoside analogs tiazofurin and selenazofurin against togaviruses, bunyaviruses, and arenaviruses. | binary combinations of the n-nucleoside ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) and the c-nucleoside analog selenazofurin (2-beta-d-ribofuranosylselenazole-4-carboxamide) or tiazofurin (2-beta-d-ribofuranosylthiazole-4-carboxamide) were tested in vitro for activity against venezuelan equine encephalomyelitis, japanese encephalitis, yellow fever, rift valley fever, korean hemorrhagic fever, and pichinde viruses. the 50% effective dose for each compound alone or in a series ... | 1984 | 6151377 |
| antigenic relationships among turlock serogroup bunyaviruses as determined by neutralization tests. | antigenic relationships between the five recognized turlock serogroup viruses (family bunyaviridae, genus bunyavirus) were determined by serum dilution-plaque reduction neutralization tests. results indicated that turlock , umbre , m' poko and lednice viruses are distinct from each other and that yaba -1 virus is a subtype of m' poko virus. | 1984 | 6203387 |
| in-vivo activity of antivirals against exotic rna viral infections. | infection of humans by viruses belonging to the families of toga-, bunya-, and arenaviridae constitutes a major health problem worldwide and certain of the viruses have the potential to cause widespread epidemics. in the search for effective chemotherapy against these viruses several hundred nucleoside and nucleotide analogues have been screened for antiviral activity. of the compounds tested, ribavirin, has been shown in laboratory animal models to have significant inhibitory effects against ri ... | 1984 | 6208183 |
| characterization of barmah forest virus: an alphavirus with some unusual properties. | barmah forest virus has been characterized in a number of ways including electron microscopy of infected cells; physical studies of the virion, its rna, and associated proteins; n-terminal sequence analysis of the two envelope glycoproteins; studies of macromolecular species present in infected cells; and serological cross-reactions with alphaviruses and bunyaviruses. from these results barmah forest virus is clearly an alphavirus since the structure of the virion, the mode of replication, and t ... | 1984 | 6324461 |
| [genome analysis of bunyavirus recombinants by dot hybridization]. | a simple method of the so-called pinpoint hybridization for the detection of genome rnas and individual fragments of genome of the bunyamwera family viruses is described. the method established linking of 2 out of 3 fragments of genome rna of different members of the family. | 1984 | 6380110 |
| mixed infections with tick-borne viruses in a seabird colony in eire. | viruses were isolated from 2 tick species collected from the nesting areas of seabirds on great saltee island, eire. bunyaviruses of the uukuniemi serogroup were isolated from hard ticks (ixodes uriae and i. rothschildi), bunyaviruses of the hughes serogroup from soft ticks (ornithodoros maritimus), and orbiviruses of the kemerovo serogroup from i. uriae and o. maritimus. the results indicate that the bunyaviruses, but not the orbiviruses, show "tick specificity". neutralising activity against m ... | 1984 | 6421266 |
| localized conserved regions of the s rna gene products of bunyaviruses are revealed by sequence analyses of the simbu serogroup aino virus. | the complete nucleotide sequence has been determined for the s rna of aino virus, a member of the simbu serogroup (bunyavirus genus, family bunyaviridae). the s rna is 850 nucleotides long (2.76 x 10(5) daltons) and in the viral complementary sequence has a short 5' non-coding region of 34 nucleotides and a more extensive 3' non-coding region of 117 nucleotides. the 3'-5' complementarity of the aino s rna is about 25 residues long, depending on the arrangement. the aino sequence predicts that, l ... | 1984 | 6532000 |
| genome subunit reassortment among bunyaviruses analysed by dot hybridization using molecularly cloned complementary dna probes. | a simple and rapid procedure for determining the genotypes of viruses has been applied to analysis of genome subunit reassortment in heterologous crosses of batai virus, bunyamwera virus, and maguari virus, three members of the bunyamwera serogroup of bunyaviruses. the procedure for determining genotype made use of specific molecular probes to identify the parental origin of the l and m rna subunits. complementary dna copies of the three rna segments of bunyamwera virus were prepared by reverse ... | 1984 | 6547258 |
| la crosse bunyavirus can mediate ph-dependent fusion from without. | lipid binding properties which are dependent on exposure to acid ph are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. cell-to-cell fusion can be used to demonstrate this function. la crosse virus, a member of the family bunyaviridae, fused bhk-21 cells from without (ffwo) upon exposure of the absorbed virus to ph 6.3 or below. a high multiplicity of infection and temperature of 37 degrees were necessary for optimum fusion. the ph r ... | 1984 | 6695500 |
| a transcript from the s segment of the germiston bunyavirus is uncapped and codes for the nucleoprotein and a nonstructural protein. | analysis of the rnas present in bhk-21 cells infected with germiston virus showed that the transcripts from the l and m segments have a size similar to that of their template, whereas two types of complementary rna are transcribed from the s segment. one, s1, is a full-length "plus" rna strand (antigenome), and the other, s2, is an incomplete plus rna strand which serves as mrna for at least the synthesis of the n protein and a virus-specific nonstructural polypeptide, p12. the 5' ends of these ... | 1984 | 6699936 |
| mechanisms of bunyavirus virulence. comparative pathogenesis of a virulent strain of la crosse and an avirulent strain of tahyna virus. | to analyze mechanisms of virulence in the california serogroup bunyaviruses, the virulent la crosse/original (lac/original) strain was compared with the avirulent tahyna/181-57 strain. in suckling mice, both viruses were lethal upon intracerebral injection but differed markedly in their neuroinvasiveness following subcutaneous injection; 20 and 20,000 plaque-forming units, respectively, were equivalent to 1 subcutaneous ld50. the sequential course of infection was followed after subcutaneous inj ... | 1984 | 6708454 |
| characterization of two recombination-complementation groups of uukuniemi virus temperature-sensitive mutants. | with the aim of isolating temperature-sensitive (ts) mutants defective in virus maturation or glycoprotein transport, uukuniemi virus, a bunyavirus, was mutagenized with n-methyl-n'-nitro-n-nitrosoguanidine. out of 13 initial clones unable to grow at 39 degrees c (non-permissive temperature), five mutants which grew to titres above 10(7) p.f.u./ml at 32 degrees c (permissive temperature) were selected for further studies. the mutants fell into two coinciding recombination-complementation groups. ... | 1984 | 6726186 |
| antibody against viruses in maternal and cord sera: non-specific inhibitors are found to higher titre on the maternal side of the circulation. | pregnancies were identified in which maternal igg antibodies against rubella virus were not detectable by single radial haemolysis. twenty paired maternal/cord sera were then tested for haemagglutination-inhibiting (hi) activity against rubella virus without kaolin pretreatment of the sera. in the absence of specific antibody, the hi activity observed could thus be ascribed to the effect of non-specific inhibitors. the hi activity in maternal sera was significantly (p less than 0.001) higher tha ... | 1984 | 6736640 |
| comparison of the sequences and coding of la crosse and snowshoe hare bunyavirus s rna species. | the sequence of the s rna of la crosse bunyavirus was deduced from analyses of dna copies cloned in the escherichia coli plasmid pbr322. the s rna is 984 nucleotides in length, has a base ratio of 31.8% u, 27.0% a, 23.2% c, and 18.0% g, and codes for two distinct gene products that are read from overlapping reading frames in the viral complementary strand. the larger gene product (n, 26.5 x 10(3) daltons) contains 235 amino acids, and the smaller gene product (nss, 10.4 x 10(3) daltons) has 92 a ... | 1983 | 6834480 |
| bunyavirus gene structure - function relationships and potential for rna segment reassortment in the vector: la crosse and snowshoe hare reassortant viruses in mosquitoes. | modern technology now permits the elucidation of viral gene contributions to virus-vector interactions. such knowledge will undoubtedly permit determination of those mechanisms which function to preserve vector-borne virus disease cycle specificity and integrity in nature. in addition, the application of genetic techniques to reassortant virus generation in vectors should permit prediction of the evolutionary and epidemic potential of viruses in nature. | 1983 | 6867030 |
| multiple leader rnas and messenger rnas are transcribed from the la crosse virus small genome segment. | nucleotide sequencing has demonstrated that the small genome segment of bunyaviruses contains the genetic information for two viral proteins (n and nss) in overlapping reading frames (akashi and bishop, 1983; cabradilla et al., in press). using 3' end-labeled genome probes, la crosse virus (lac) infected cells were shown to contain three leader rnas, which start at position 1 and terminate at approximate positions 74, 95, and 115 from the 3' end of the genome. primer extension and s1 mapping stu ... | 1983 | 6871993 |
| [conditions for preserving the simbu group of bunyaviruses]. | 1983 | 6670261 | |
| identification of new guama and group c serogroup bunyaviruses and an ungrouped virus from southern brazil. | from 1975 to 1978, 36 viruses were recovered from humans, bats, birds, sentinel mice and hamsters, and from mosquitoes collected in coastal brazil in the state of são paulo. identifications of 22 of these 36 viruses have been reported. six of the remaining 14 isolates were shown to be guama serogroup bunyaviruses. two of these six were strains of a newly recognized virus for which the name cananeia virus is proposed; another is a second newly recognized guama serogroup virus for which the name i ... | 1983 | 6404190 |
| characterization of leanyer virus: resemblance to bunyavirus. | the properties of leanyer virus, isolated in northern australia in 1974, were compared with those of bunyamwera virus. leanyer virus replicated in bhk-21 and vero cells. in sucrose gradients it had a density of 1.17 g/cm3 and sedimented with the same s value as bunyamwera virus. the diameter of negatively stained virions was approximately 110 nm. three species of rna sedimenting at 30s (l), 26s (m) and 14s (s) and four virion proteins (l, g1, g2, n) were detected in preparations of purified viri ... | 1983 | 6411054 |
| the mapputta group of arboviruses: ultrastructural and molecular studies which place the group in the bunyavirus genus of the family bunyaviridae. | we have characterized members of the mapputta group of 'bunyavirus-like' viruses in terms of morphology, structure, ultrastructural development and virus-directed rna and protein synthesis. our primary study has been with maprik virus (mpk) as a representative of the group. the mpk virion is uniformly spherical (congruent to 90 nm diameter) and possesses a membrane envelope. virus maturation is by budding into small vesicles in the perinuclear region. during infection of bhk cells which is cytop ... | 1983 | 6411055 |
| analysis of hantaan virus rna: evidence for a new genus of bunyaviridae. | hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family bunyaviridae. nucleocapsid rna from hantaan virions and a control bunyavirus were examined for ribonuclease a (rnase a) sensitivity. both viruses exhibited a similar accessibility of rna within nucleocapsids to digestion by rnase a. complete digestion of the rna of both viruses was affected with high c ... | 1983 | 6419460 |
| [effect of ribavirin on bunyavirus reproduction in cell culture and in an experiment on white mice]. | 1983 | 6419466 | |
| taxonomy, classification, and geographic distribution of california serogroup bunyaviruses. | 1983 | 6346334 | |
| the effect of proteolytic cleavage of la crosse virus g1 glycoprotein on antibody neutralization. | the envelope of the bunyavirus la crosse contains two glycoproteins, g1 (120 000 mol. wt.) and g2 (38 000 mol. wt.). when incubated with trypsin or plasmin, the g1 glycoprotein of virus grown in cell culture was cleaved, leaving two different sized polypeptides in the envelope (67 000 and 95 000 mol. wt.). chymotrypsin cleaved g1 leaving polypeptides of 70 000 and 100 000 mol. wt. g2, however, was not altered by these enzymes. when used in antibody neutralization studies, these proteolytically m ... | 1983 | 6352863 |
| interference between bunyaviruses in aedes triseriatus mosquitoes. | inhibition of the replication of alternate california serogroup bunyaviruses in aedes triseriatus mosquitoes has been observed for mosquitoes previously infected with la crosse (lac) virus. by contrast, prior infection of mosquitoes with lac virus did not interfere significantly with the subsequent infection and replication of guaroa bunyavirus (bunyamwera serogroup), or heterologous viruses such as west nile flavivirus, or vesicular stomatitis rhabdovirus. | 1983 | 6305019 |
| identification of hitherto unrecognized arboviruses from ecuador: members of serogroups b, c, bunyamwera, patois, and minatitlan. | three hundred seventy-nine virus isolates were obtained from mosquitoes collected and sentinel hamsters exposed in coastal ecuador from 1974 to 1978. these included four alphaviruses [venezuelan equine encephalitis 1b (1), venezuelan equine encephalitis 1d (35), western equine encephalitis (1) and eastern equine encephalitis (4)]; two flaviviruses [st. louis encephalitis (3) and naranjal (6)]; 11 bunyaviruses [maguari (243), playas (3), vinces (33), turlock (2), abras (5), babahoyo (3), acara (2 ... | 1983 | 6309029 |
| antiviral activity released from aedes albopictus cells persistently infected with semliki forest virus. | aedes albopictus (mosquito) cells persistently infected with semliki forest virus released an agent which inhibited virus production by a. albopictus cells infected with homologous virus. inhibition of virus production was accompanied by a marked reduction in the synthesis of viral rna and viral proteins. expression of the antiviral effect was prevented by pretreatment of cells with actinomycin. no analogous antiviral activity was detected in culture fluids of a. albopictus cells persistently in ... | 1983 | 6312089 |
| nonviral heterogeneous sequences are present at the 5' ends of one species of snowshoe hare bunyavirus s complementary rna. | analyses of the 5' ends of snowshoe hare bunyavirus plus sense s rna species (including mrna) recovered from infected cells have revealed two types of termini. these include ends that are essentially exact copies of the 3' end of the viral s rna and others that are similar, but additionally have 13-14 nucleotide extensions that are heterogeneous in sequence. the former probably represent replicative plus sense rna species, the latter mrna species that have host cell derived primer sequences. | 1983 | 6312422 |
| viruses of classical and mild forms of haemorrhagic fever with renal syndrome isolated in china have similar bunyavirus-like morphology. | 1983 | 6131283 | |
| yukon isolates of snowshoe hare virus, 1972-1982. | bunyaviruses including 53 strains of snowshoe hare (ssh) and 4 of northway (nor) were isolated from 132,428 unengorged adult female mosquitoes of 7 species collected throughout the boreal forest of the yukon territory and open woodland terrain in the mackenzie valley, northwest territories, canada during 8 of 11 arctic summers from 1972 through 1982. isolations of ssh virus were also achieved from mosquito larvae during 1974 and 1975. percentage virus infection rates of important vectors were ae ... | 1983 | 6135219 |
| radial haemolysis in gel for detection of antibodies to bunyavirus lednice (turlock group). | the method of radial haemolysis in gel (rhg) was used for detection of antibodies to lednice virus. repeated and comparative experiments proved that the method gave reliable and sufficiently specific standard results. no cross-reactions were detected with sera to other arboviruses. comparison of antibody titres achieved in rhg with those of haemagglutination inhibition (hit) and indirect immunofluorescence (if) tests showed a satisfactory sensitivity, as the antibody titres in rhg and hit were p ... | 1983 | 6139949 |
| bunyavirus nucleoprotein, n, and a non-structural protein, nss, are coded by overlapping reading frames in the s rna. | it has been shown previously, by sequence analysis of the s rna segment of snowshoe hare (ssh) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 x 10(3) and 10.5 x 10(3) respectively. in addition to the viral nucleocapsid (n) protein, which is coded by the s rna, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral s rna and m rna species each code for non-structura ... | 1983 | 6223987 |
| [human disease caused by nepuyo virus, a central american bunyavirus transmitted by mosquitoes]. | 1983 | 6226296 | |
| [mechanisms of in vivo suppressive effect of togaviridae and bunyaviridae on the activity of effectors of graft vs host reaction]. | experiments on mice demonstrated the ability of 3 flaviviruses and 1 bunyavirus to suppress the activity of the effectors of the graft-versus-host (gvh) reaction. the conditions of the suppression of the primary immunological recognition were shown to differ in infections caused by different viruses. in experimental flavivirus infections caused by langat, dengue 2 or yellow fever (strain 17d) viruses t-suppressor cells were activated, and their activity was realized only in respect to syngeneic ... | 1982 | 6283771 |
| orbi- and bunyaviruses from a puffin colony in the outer hebrides. | 1982 | 6299238 | |
| viruses recovered from mosquitoes and wildlife serum collected in the murray valley of south-eastern australia, february 1974, during an epidemic of encephalitis. | pools of mosquitoes collected in the murray valley in february, 1974, during an encephalitis epidemic yielded 239 isolates of 11 distinct viruses. these included 39 isolates of mve virus, an incriminated causative agent of encephalitis in man, and 111 isolates of kunjin virus, a probable causative agent. an additional isolate of mve virus was recovered from the serum of a white-faced heron, ardea novaehollandiae. the other 9 viruses comprised the alpha-viruses ross river and sindbis, the flavivi ... | 1982 | 6299258 |
| sensitivity to carbon dioxide in mosquitoes infected with california serogroup arboviruses. | ten species of mosquitoes became sensitive to co2 following intrathoracic (i.t.) inoculation of california encephalitis (ce) virus. these included field-collected aedes melanimon, aedes nigromaculis and culiseta incidens and laboratory-colonized strains of aedes dorsalis, aedes triseriatus, anopheles freeborni, culex peus, culex pipiens pipiens, culex pipiens quinquefasciatus and culex tarsalis. another california serogroup virus, jerry slough (= jamestown canyon) (js), also induced co2 sensitiv ... | 1982 | 6122383 |
| [characteristics of bunyavirus- and togavirus-induced nonspecific suppressors inhibiting delayed hypersensitivity]. | some properties and mechanisms of action of nonspecific suppressor cells, inhibiting delayed hypersensitivity to sheep red blood cells and activated in vivo in experimental tick-borne encephalitis and tahyna virus infections in mice, have been studied. these nonspecific suppressor cells have been identified as t-lymphocytes in experiments with the use of antisera to t- and b-lymphocytes. the function of the suppressor cells can be realized without their proliferation and is mediated by a soluble ... | 1982 | 6213121 |
| relationship between glycoproteins of the viral envelope of bunyaviruses and antibody-dependent plaque enhancement. | hamster antisera against three parental bunyaviruses, batai, bunyamwera and maguari viruses, and six recombinant viruses which carried the nucleocapsid protein of one parent and the glycoproteins of the other, have been tested for their interaction with each of the nine viruses under study by two assays, plaque reduction neutralization and antibody-dependent plaque enhancement. neutralization was clearly related to the specificity of the parental glycoproteins rather than the nucleoprotein, but ... | 1982 | 6890986 |
| molecular basis of bunyavirus per os infection of mosquitoes: role of the middle-sized rna segment. | the molecular basis of bunyavirus per os infection of mosquitoes was determined; la crosse (lac), snowshoe hare (ssh), and lac-ssh reassortment viruses were compared for their ability to infect aedes triseriatus, the natural vector of the lac virus. the viruses were comparable in their ability to infect midgut cells; 115 of 117 (98%) mosquitoes ingesting viruses containing the lac middle-sized rna segment and 92/100 (92%) of mosquitoes ingesting viruses containing the ssh middle-sized rna segmen ... | 1982 | 6951175 |
| seven infection-specific polypeptides in bhk cells infected with bunyamwera virus. | virus-specific polypeptide synthesis was examined in bhk cells and vero cells infected with bunyamwera virus. in bhk cells, in addition to the four previously reported virus-coded proteins (l, g1, g2, and n), three other infection-specific proteins were detected. these proteins, of nominal molecular weight 50,000 (p50), 16,000 (p16), and 13,000 (p13), were not labeled in mock-infected cells, were first synthesized between 4 and 8 h after infection, and were relatively prominent among the limited ... | 1982 | 6183441 |
| the complete sequence and coding content of snowshoe hare bunyavirus small (s) viral rna species. | the complete sequence of the small (s) viral rna species of snowshoe hare (ssh) bunyavirus has been determined, principally from a dna copy of the rna cloned in the e.coli plasmid pbr322. the viral s rna (negative sense strand) is 982 nucleotides long (3.3 x 10(5) daltons) with complementary 5' and 3' end sequences. it has a base composition of 30.5%u, 25.8%a, 24.9%c and 18.7%g. in the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine c ... | 1982 | 7050911 |
| identification of virus-coded nonstructural polypeptides in bunyavirus-infected cells. | analyses of bunyavirus-infected cell extracts identified at least two virus-induced nonstructural polypeptides. with snowshoe hare (ssh), la crosse (lac), and six ssh-lac reassortant viruses, it was shown that one of these nonstructural polypeptides (nss, approximate molecular weight, 7.4 x 10(3)) is coded by the ssh small (s)-size viral rna species. this nonstructural polypeptide was not detected (at least in the same relative abundancies) in lac virus-infected cells or in cells infected with r ... | 1982 | 7077749 |
| nucleotide sequence analyses and predicted coding of bunyavirus genome rna species. | we performed 3' rna sequence analyses of [(32)p]pcp-end-labeled la crosse (lac) virus, alternate lac virus isolate l74, and snowshoe hare bunyavirus large (l), medium (m), and small (s) negative-stranded viral rna species to determine the coding capabilities of these species. these analyses were confirmed by dideoxy primer extension studies in which we used a synthetic oligodeoxynucleotide primer complementary to the conserved 3'-terminal decanucleotide of the three viral rna species (clerx-van ... | 1982 | 7086954 |
| electron microscopic study of bluegill virus. | bluegill virus (bgv) grown in bf-2 cells was studied by negative staining and ultrathin sections of infected cells. although bgv resembles bunyaviruses in gross physical appearance, it differs from this group in several important aspects. thus, bgv cannot be classified as a member of the bunyaviridae family and could be a representative of a novel family of enveloped rna viruses. | 1982 | 7093819 |
| mutant identifying a third recombination group in a bunyavirus. | only two recombination groups have been reported in genetic analyses of ts mutants of 10 different bunyaviruses from the bunyamwera and california encephalitis serogroups, although three groups are expected from the tripartite structure of the genome of all members of the family bunyaviridae. we describe now a ts mutant of maguari virus, magts23(iii), which recombined in both vertebrate (bhk-21) and invertebrate (aedes albopictus) cells with mutants representing recombination groups i and ii of ... | 1982 | 7097862 |
| characterization of monoclonal antibodies against the g1 and n proteins of lacrosse and tahyna, two california serogroup bunyaviruses. | 1982 | 7101727 | |
| the 3' terminal rna sequences of bunyaviruses and nairoviruses (bunyaviridae): evidence of end sequence generic differences within the virus family. | the 3' terminal nucleotide sequences of the three virus rna species of viruses representing eight serogroups of bunyaviruses (genus bunyavirus, bunyaviridae) and six serogroups of nairoviruses (genus nairovirus, bunyaviridae) have been characterized. members of the bunyavirus genus have conserved 3' end sequences (generally, 3' ucaucacauga...) that differ from the conserved 3' end sequences of members of the nairovirus genus (generally, 3' agaguuucu...). | 1982 | 7119754 |
| panhandles and hairpin structures at the termini of germiston virus rnas (bunyavirus). | 1982 | 7135833 | |
| characterization of the oligosaccharides of inkoo virus envelope glycoproteins. | inkoo virus (a bunyavirus) was grown in bhk-21 cells and labelled with [35s]methionine or [3h]mannose. [35s]methionine labelled the two envelope glycoproteins g1 (mr = 125000) and g2 (mr = 35000), as well as the nucleocapsid protein n (mr = 25000). only g1 and g2 were labelled with the sugar precursor. the [3h]mannose-labelled virus was solubilized with detergent and digested with pronase. the structure of the labelled glycopeptides originating from the mixture of g1 and g2 was studied by degrad ... | 1982 | 7153764 |
| analyses of patois group bunyaviruses: evidence for naturally occurring recombinant bunyaviruses and existence of immune precipitable and nonprecipitable nonvirion proteins induced in burnyavirus-infected cells. | 1981 | 7222477 | |
| attenuation of virulence of a bunyavirus involving an l rna defect and isolation of lac/ssh/lac and lac/ssh/ssh reassortants. | 1981 | 7233829 | |
| formation of reassortant bunyaviruses in dually infected mosquitoes. | 1981 | 7245612 | |
| radioimmune assays and molecular studies that place anopheles b and turlock serogroup viruses in the bunyavirus genus (bunyaviridae). | molecular analyses indicate that turlock virus (tur, turlock serogroup) and boraceia virus (bor, anopheles b serogroup) have virion rna species and polypeptides comparable in size to those of members of the bunyavirus genus and unlike those of members of the newly defined phlebovirus, nairovirus, or uukuvirus genera (bunyaviridae). the 11 terminal 3' end nucleotides of the three virion rna species of both bor and tur viruses (houcaucacaug...) are identical in sequence to the 3' end sequences of ... | 1981 | 7258499 |
| structural polypeptides of hazara virus. | four structural polypeptides of hazara virus, an agent closely related to the crimean-congo haemorrhagic fever (c-chf) viruses, were resolved by sds-polyacrylamide gel electrophoresis. three glycoproteins were identified (mol. wt. 84,000, 45,000 and 30,000) and were found to be associated with the virion envelope. a fourth polypeptide (mol. wt. 52,000) was non-glycosylated and associated with the nucleocapsid. the structural proteins of hazara virus differ markedly from those reported for other ... | 1981 | 7276913 |
| role of the middle-sized bunyavirus rna segment in mouse virulence. | 1981 | 7281516 | |
| comparisons of belmont virus, a possible bunyavirus unique to australia, with bunyamwera virus. | belmont virus is an arbovirus isolated from mosquitoes and has a preference for marsupial hosts. the diameter of virions by negative staining (122 nm before fixation and 91 nm after fixation) was greater than that of bunyamwera virus (94 nm and 79 nm respectively). however, the particles of both viruses appeared morphologically identical and sedimented at the same rate in sucrose density gradients. belmont virus had a tripartite segmented rna genome (28s, 24s and 11s) similar to bunyamwera virus ... | 1981 | 7288402 |
| proteins and glycoproteins specified by bunyamwera virus and by belmont virus, a possible bunyavirus, in mammalian cells. | purified preparations of belmont virus were shown to be very similar morphologically and biochemically to those of bunyamwera virus. comparisons of the synthesis of virus-specified proteins in vero and bhk-21 cells confirm the close taxonomic relationship. total protein synthesis was inhibited 95% by 23 h post-infection with belmont virus; a similar reduction occurred earlier in bunyamwera virus-infected cells. this inhibition was multiplicity dependent, and synthesis of the host protein compone ... | 1981 | 7288403 |
| structural characteristics of nairoviruses (genus nairovirus, bunyaviridae). | viruses from six antigenic groups of arthropod-borne viruses [crimean--congo haemorrhagic fever (cchf), nairobi sheep disease (nsd), qalyub (qyb), sakhalin (sak), dera ghazi khan (dgk) and hughes (hug) serogroups], some previously categorized as bunyavirus-like viruses and others previously ungrouped, have recently been assembled by serological analyses into a new genus of viruses (nairovirus genus) in the bunyaviridae. molecular studies of the virion rna and viral polypeptides have been underta ... | 1981 | 7299367 |
| orbiviruses and bunyaviruses from a seabird colony in scotland. | viruses isolated from ticks (ixodes uriae) and a kittiwake (rissa tridactyla) from a seabird colony at st. abb's head, scotland, were shown by complement fixation tests (cft) to be antigenically related to the uukuniemi and kemerovo serogroups. electron microscopic examination of cell cultures infected with the kemerovo group viruses revealed particles characteristic of orbiviruses, 72 +/- 3 nm in diam., with an inner core 37 +/- 3 nm in diam., in association with intracytoplasmic, densely stain ... | 1981 | 7320703 |
| antigenic relationships among simbu serogroup (bunyaviridae) viruses. | antigenic relationships among 24 bunyaviruses of the simbu serogroup were determined by complement-fixation (cf), serum dilution-plaque reduction neutralization (n) and, where possible, hemagglutination-inhibition (hi) tests. by cf, three distinct complexes of closely related viruses were identified within the serogroup. nola and thimiri viruses, which showed little relationship with other members of the serogroup, may represent two additional complexes. n tests in vero cells showed that individ ... | 1981 | 7325288 |
| monoclonal anti-fc receptor igg blocks antibody enhancement of viral replication in macrophages. | flaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes (ref. 1, and j.s.m.p. and j.s.p., unpublished observations) and in p388 d1 and other macrophage cell lines. a comparable phenomenon has been demonstrated with alphaviruses and bunyaviruses in p388 d1 cells, (j.s.m.p. and j.s.p., unpublished observations) but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ade ... | 1981 | 7453820 |
| molecular basis of bunyavirus transmission by mosquitoes: role of the middle-sized rna segment. | in an examination of the molecular basis of oral transmission of bunyaviruses by mosquitoes., la crosse (lac), snowshoe hare (ssh), and lac-ssh reassortant viruses were compared in their ability to be transmitted to laboratory mice by the natural mosquito vector of lac virus, aedes triseriatus. both lac virus and the reassortment viruses containing the middle-sized (m) segment from the lac parent were efficiently transmitted. in contrast, ssh virus and reassortment viruses containing the m rna f ... | 1981 | 6781068 |
| [bunyaviruses]. | 1981 | 6798775 | |
| [transovarial transmission of arboviruses by mosquitoes (author's transl)]. | an important aspect of the epidemiology of arboviruses is the manner in which the viruses are maintained during winter, dry season, or other adverse environmental periods when their arthropod hosts are inactive. one possibility is that the viruses survive in arthropods. in the case of mosquito-borne viruses, it is probable that such viruses could be maintained in this manner only if they were transmitted from one insect generation to the next by transovarial transmission. such transmission was r ... | 1981 | 6116146 |
| guamá-group virus activity in surinam. | from october 1972 through december 1973 entomological surveys were carried out in both a savannah and coastal area in surinam. attempts were made to isolate arboviruses from the captured mosquitoes. the results have been published elsewhere (panday and digoutte, 1979). the present paper deals with the further typing of the obtained guamá-group viruses. preliminary typing was done by means of complement-fixation test. by performing the neutralization test in mice, the unknown guamá-group viruses ... | 1981 | 6116303 |
| use of hyperimmune mouse ascitic fluids for arbovirus differentiation by indirect immunofluorescence and conventional serology. | the cross-reactivity of 22 arbovirus species (alphaviruses; tick- and mosquito-borne flaviviruses; and selected bunyaviruses) was tested with monovalent immune mouse ascitic fluids by indirect immunofluorescence (iif) in comparison with classical serological reactions (virus neutralization -- vn; haemagglutination inhibition -- hi; and complement fixation -- cf -- reactions). known relationships within the virus groups studied were confirmed. as to the differentiation limits, the vn test was fol ... | 1981 | 6118048 |
| analyses of patois group bunyaviruses: evidence for naturally occurring recombinant bunyaviruses and existence of immune precipitable and nonprecipitable nonvirion proteins induced in bunyavirus-infected cells. | shark river (sr) and pahayokee (pah) bunyaviruses (patois serogroup, bunyavirus genus, family bunyaviridae) have almost identical l and s rna oligonucleotide fingerprints, but m rna fingerprints that are different, suggesting that the two viruses may represent naturally occurring reassortant viruses. these observations are in agreement with serological studies (b. n. fields, b. e. henderson, p. h. coleman, and t. h. work, 1969, amer. j. epidemiol., 89, 222-226) which have distinguished these two ... | 1981 | 18635048 |
| arboviruses of coastal south-eastern australia. | during investigations of epidemic polyarthritis at nelson bay, new south wales, 12 strains of ross river virus, the causative agent, were recovered from pools of mosquitoes. in addition, the mosquito pools yielded 9 strains of the flavivirus edge hill, 4 strains of a bunyavirus, gan gan, 1 strain of an orbivirus tilligerry, and 1 strain of an ungrouped probable arbovirus, yacaaba. the latter 3 viruses were found to be antigenically distinct from previously recorded arboviruses. a case of epidemi ... | 1980 | 6108758 |
| bunyaviridae. | the family bunyaviridae comprises over 200 viruses (serotypes, subtypes, and varieties) that infect vertebrates and/or invertebrates. four genera of viruses have been defined (bunyavirus, nairovirus, phlebovirus, and uukuvirus). the main characteristics of the member viruses are: (i) the virus particles are for the most part uniformly spherical, 80-110 nm in diameter, and possess a unit membrane envelope from which protrude polypeptide spikes 5-10nm long; (ii) the viruses have three helical nucl ... | 1980 | 6165702 |
| recombination and gene coding assignments of bunyaviruses and arenaviruses. | 1980 | 7013623 | |
| carbon dioxide sensitivity of mosquitoes infected with california encephalitis virus. | four species of mosquitoes became sensitive to carbon dioxide approximately 3 to 4 days after they received intrathoracic injectins of california encephalitis virus. aedes melanimon and aedes dorsalis infected orally with california encephalitis virus also became carbon dioxide-sensitive, but mosquitoes infected transovarially did not. sensitivity to carbon dioxide was inhibited by antiserum to california encephalitis virus. to our knowledge this is the first report of carbon dioxide sensitivity ... | 1980 | 6773145 |
| tataguine virus infection in mice and hamsters. | suckling hamsters, weanling and suckling mice, inoculated intracerebrally with tataguine virus consistently developed fatal infections with average survival time of 3-8 days. deaths were observed in a few suckling hamsters infected by the intraperitoneal route. infectivity assays and fluorescent antibody staining of organs of infected hamsters showed that the brain was the only organ in which viral replication occurred. histopathological lesions found in the brain of such mice and hamsters infec ... | 1980 | 6776388 |
| characterization of the viral ribonucleic acids and structural polypeptides of anopheles a, bunyamwera, group c, california, capim, guama, patois, and simbu bunyaviruses. | analyses of the viral ribonucleic acids and structural polypeptides of 17-22 of the 119 accepted or proposed members of the bunyavirus genus of arboviruses (family bunyaviridae), have shown that from the standpoint of their structural components these viruses are highly comparable to each other. the average molecular weights for the three viral rna species (l, large, m, medium, s, small) of 17 bunyaviruses were 2.93 x 10(6) (l, range 2.7-3.1 x 10(6)), 2.0 x 10(6) (m, range 1.8-2.3 x 10(6)), and ... | 1980 | 6778231 |
| evidence from recombinant bunyavirus studies that the m rna gene products elicit neutralizing antibodies. | 1980 | 7368566 | |
| the effect of glycosylation inhibitors on the maturation and intracellular polypeptide synthesis induced by snowshoe hare bunyavirus. | 1980 | 7368577 | |
| analyses of the 3'-terminal sequences of snowshoe hare and la crosse bunyaviruses. | 1980 | 7423860 | |
| tonate and guama-group viruses isolated from mosquitoes in both a savannah and coastal area in surinam. | isolation of arboviruses was attempted from october 1972 till the end of 1973 from mosquitoes caught in both a savannah forest near powaka and a coastal area near "fernandesweg" in surinam. two types of arbovirus were isolated: eleven strains of tonate and ten strains of guama-group. in the savannah area ten strains of tonate and five of guama-group were isolated, whereas in the coastal area the numbers were one and five, respectively. all strains in the savannah area were isolated from pools of ... | 1979 | 41349 |
| bunyavirus development in arctic and aedes aegypti mosquitoes as revealed by glucose oxidase staining and immunofluorescence. | northway virus replication has been detected in salivary glands of wild-caught culiseta inornata and aedes communis mosquitoes from the western canadian arctic after incubation at 4 degrees c for 9 to 11 months, and after incubation at 13 degrees c for 3 to 4 months after they received virus by oral ingestion or intrathoracic injection. aedes hexodontus supported northway virus replication after incubation at 13 degrees c for one month after intrathoracic injection. aedes aegypti supported north ... | 1979 | 44464 |
| genetic potential of bunyaviruses. | 1979 | 387343 | |
| study of arboviruses in romania (1958--1978). results obtained in the "stefan s. nicolau" institute of virology. | the results obtained in the institute of virology by the study of arboviruses in romania are reviewed. isolation of tick-borne encephalitis virus and detection of specific serum antibodies in humans, animals and migratory birds demonstrate its presence on the territory of romania. serum antibodies could also be detected to other three flaviviruses (west nile, ntaya, dengue types 1, 2, 3), though none of them could be isolated in our country. however, isolation of kemerovo and tahyna viruses and ... | 1979 | 392912 |
| a comparison of la crosse virus isolated obtained from different ecological niches and an analysis of the structural components of california encephalitis serogroup viruses and other bunyaviruses. | analyses of the oligonucleotide fingerprints of the three genome ribonucleic acid (rna) species of 11 isolates of la crosse (lac) virus, obtained from various ecological niches in the northern united states and compared to those of prototype lac virus, showed that in each place from which these isolates were obtained lac variants and varieties were present with related, but distinguishable, nucleotide sequences for their large, medium, or small rna species. the rna genomes of prototypes trivitta ... | 1979 | 453439 |
| heterologous interference in aedes albopictus cells infected with alphaviruses. | maximum amounts of 42s and 26s single-stranded viral rna and viral structural proteins were synthesized in aedes albopictus cells at 24 h after sindbis virus infection. thereafter, viral rna and protein syntheses were inhibited. by 3 days postinfection, only small quantities of 42s rna and no detectable 26s rna or structural proteins were synthesized in infected cells. superinfection of a. albopictus cells 3 days after sindbis virus infection with sindbis, semliki forest, una, or chikungunya alp ... | 1979 | 480461 |
| m viral rna segment of bunyaviruses codes for two glycoproteins, g1 and g2. | tryptic peptide digests of the two viral glycoproteins (g1 and g2) of snowshow hare (ssh) virus, la crosse, la crosse (lac) virus, and an ssh/lac recombinant virus which has a large (l)/medium (m)/small (s) rna segment genome composition of ssh/lac/ssh were analyzed by ion-exchange column chromatography. the analyses prove that the m rna species of bunyaviruses codes for the two viral glycoproteins. | 1979 | 480466 |
| in vivo transcription and protein synthesis capabilities of bunyaviruses: wild-type snowshoe hare virus and its temperature-sensitive group i, group ii, and group i/ii mutants. | the in vivo primary and secondary transcription capabilities of wild-type snowshoe hare (ssh) virus and certain of its temperature-sensitive (ts) mutants have been analyzed. the results obtained agree with in vitro studies (bouloy et al., c.r. acad. sci. paris 280:213-215, 1975; m. bouloy and c. hannoun, virology 69:258-264, 1976; m. ranki and r. pettersson, j. virol. 16:1420-1425, 1975) which have shown that bunyaviruses are negative-stranded rna viruses with a virion rna-directed rna polymeras ... | 1979 | 480477 |
| genome complexities of the three mrna species of snowshoe hare bunyavirus and in vitro translation of s mrna to viral n polypeptide. | the genome complexities of the principal intracellular viral complementary rna species of the snowshoe hare bunyavirus have been analyzed by duplex analyses involving hybridization of complementary rna to individual 32p-labeled viral rna species (large, l; medium, m; and small, s), recovery of nuclease-resistant duplexes, and determination of the oligonucleotide fingerprints of the protected 32p-labeled viral sequences. the result for the m rna (which codes for the glycoproteins g1 and g2; j. r. ... | 1979 | 513192 |
| recombination between snowhoe hare and la crosse bunyaviruses. | we have previously reported heterologous genetic recombination resulting from crosses involving temperature-sensitive (ts) mutants of la crosse (lac) group ii and snowshoe hare (ssh) group i ts mutants (j. gentsch, l. r. wynne, j. p. clewley, r. e. shope, and d. h. l. bishop, j. virol. 24:893-902, 1977). from those crosses two reassortant viruses having the large/medium/small viral rna segment genotypes of ssh/lac/ssh and ssh/lac/lac were obtained. in this study it has been found that the recipr ... | 1979 | 513193 |