Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| emerging roles of chicken and viral micrornas in avian disease. | abstract: | 2011 | 21645299 |
| development and application of a phosphoproteomic method using electrostatic repulsion-hydrophilic interaction chromatography (erlic), imac, and lc-ms/ms analysis to study marek's disease virus infection. | marek's disease (md) is an avian neoplastic disease caused by marek's disease virus (mdv). the mechanism of virus transition between the lytic and latent cycle is still being investigated; however, post-translational modifications, especially phosphorylation, have been thought to play an important role. previously, our group has used strong cation exchange chromatography in conjunction with reversed-phase liquid chromatography-tandem mass spectrometry (lc-ms/ms) to study the changes in global pr ... | 2011 | 21736374 |
| transcriptional profiling of host gene expression in chicken liver tissues infected with oncogenic marek's disease virus. | summarymarek's disease virus (mdv), one of the most potent oncogenic herpesviruses, leads to highly contagious immunosuppressive and neoplastic disease in susceptible chickens. previous studies mainly focused on the roles of host genes modulated by mdv in the virological rather than neoplastic stage. to further investigate the molecular mechanisms of tumorigenesis in marek's disease (md) system, a microarray analysis with affymetrix gene-chip chicken genome arrays was performed in a non-lymphoid ... | 2011 | 21832007 |
| heat-shock protein 70 is associated with the entry of marek's disease virus into fibroblast. | literature pertaining to the interactions between marek's disease virus (mdv) entry-related glycoproteins and corresponding receptors is still limited. results from a western blot analysis of cellular proteins for virus receptors and co-immunoprecipitation suggest that heat shock protein 70 (hsp70) is a potential cellular receptor for mdv glycoprotein gh. plaque inhibition assays confirm the involvement of hsp70 in the early stages of mdv entry into chicken embryo fibroblasts (cef). the present ... | 2011 | 21978152 |
| rapid detection of marek's disease virus in feather follicles by loop-mediated amplification. | marek's disease (md) remains a serious problem in the production of poultry. the disease is caused by marek's disease virus (mdv), and despite the ubiquitous use of vaccination to control losses, md still affects poultry farming worldwide. the aim of this study was to develop a loop-mediated isothermal amplification (lamp) method for the simple and inexpensive detection of mdv in feather tips of chickens. two pairs of specific primers complementary to the meq oncogene of mdv were designed, targe ... | 2011 | 22017048 |
| gene expression profiling in rmd5- and rmd5deltameq-infected chickens. | marek's disease (md) is a lymphoproliferative disorder of domestic chickens caused by a highly contagious and oncogenic alpha-herpesvirus, marek's disease virus (mdv). md is characterized by bursal-thymic atrophy and rapid onset of t-cell lymphomas that infiltrate lymphoid tissues, visceral organs, and peripheral nerves with severe clinical signs that include transient paralysis, anemia, weight loss, and neurologic disorders. using overlapping cosmids- and bac-cloned mdv, it has been shown that ... | 2011 | 22017031 |
| surveillance of marek's disease virus in migratory and sedentary birds in hokkaido, japan. | marek's disease virus serotype 1 (mdv-1) strains cause malignant lymphoma in chickens. mdv-1 has been previously reported to be widespread in white-fronted geese (anser albifrons); however, the prevalence of mdv-1 in other wild birds has not been determined. in this study, we investigated the prevalence of mdv-1 in various wild birds in hokkaido, japan. the mdv-1 genome was widespread in geese and ducks, but was not detected in other birds. mdv-1 was detected in both sedentary and migratory spec ... | 2011 | 21908212 |
| modelling marek's disease virus (mdv) infection: parameter estimates for mortality rate and infectiousness. | marek's disease virus (mdv) is an economically important oncogenic herpesvirus of poultry. since the 1960s, increasingly virulent strains have caused continued poultry industry production losses worldwide. to understand the mechanisms of this virulence evolution and to evaluate the epidemiological consequences of putative control strategies, it is imperative to understand how virulence is defined and how this correlates with host mortality and infectiousness during mdv infection. we present a ma ... | 2011 | 22078942 |
| comparative full-length sequence analysis of marek's disease virus vaccine strain 814. | the complete dna sequence of marek's disease virus (mdv) serotype 1 vaccine strain 814 was determined. it consisted of 172,541 bp, with an overall gene organization identical to that of the mdv-1 type strains. comparative genomic analysis of vaccine strains (814 and cvi988) and other strains (cu-2, md5, and md11) showed that 814 was most similar to cvi988. several unique insertions, deletions, and substitutions were identified in strain 814. of note, a 177-bp insertion in the overlapping genes e ... | 2011 | 21984218 |
| Herpesvirus telomerase RNA (vTR) with a mutated template sequence abrogates herpesvirus-induced lymphomagenesis. | Telomerase reverse transcriptase (TERT) and telomerase RNA (TR) represent the enzymatically active components of telomerase. In the complex, TR provides the template for the addition of telomeric repeats to telomeres, a protective structure at the end of linear chromosomes. Human TR with a mutation in the template region has been previously shown to inhibit proliferation of cancer cells in vitro. In this report, we examined the effects of a mutation in the template of a virus encoded TR (vTR) on ... | 2011 | 22046133 |
| roles of avian herpesvirus micrornas in infection, latency, and oncogenesis. | micrornas have been reported for the avian herpesviruses marek's disease virus 1 (mdv1; oncogenic), marek's disease virus 2 (mdv2; non-oncogenic), herpesvirus of turkeys (hvt), and infectious laryngotracheitis virus (iltv). no obvious phylogenetic relationships exist among the avian herpesvirus micrornas, but the general genomic locations of microrna clusters are conserved, with micrornas being located in the repeat regions of the genomes. in some cases, micrornas are antisense to open reading f ... | 2011 | 21683170 |
| systems analysis of immune responses in marek's disease virus-infected chickens identifies a gene involved in susceptibility and highlights a possible novel pathogenicity mechanism. | marek's disease virus (mdv) is a highly contagious oncogenic alphaherpesvirus that causes disease that is both a cancer model and a continuing threat to the world's poultry industry. this comprehensive gene expression study analyzes the host response to infection in both resistant and susceptible lines of chickens and inherent expression differences between the two lines following the infection of the host. a novel pathogenicity mechanism, involving the downregulation of genes containing hic1 tr ... | 2011 | 21865384 |
| mirna expression signatures induced by marek's disease virus infection in chickens. | micrornas (mirnas) are small, non-coding rnas that regulate gene expression at the post-transcriptional level. emerging evidence suggests that differential mirna expression is associated with viral infection and cancer. marek's disease virus infection induces lymphoma in chickens. however, the host defense response against marek's disease (md) progression remains poorly understood. here, we utilized microarrays to screen mirnas that were sensitive to marek's disease virus (mdv) infection. qrt-pc ... | 2011 | 22178800 |
| rapid detection of marek's disease viral genome in chicken feathers by loop mediated isothermal amplification (lamp). | a loop-mediated isothermal amplification (lamp) method for the rapid detection of serotype 1 marek's disease virus (mdv) was developed. the method used a set of three pairs of primers to amplify the meq gene for detecting serotype 1 mdv. the mdv lamp method did not cross-react with serotype 2 and serotype 3, nor did the lamp primers have binding sites for the common avian dna viruses (reticuloendotheliosis virus, chicken anemia virus, subgroup j of the avian leucosis virus). additionally, the as ... | 2011 | 22170920 |
| Fluorescently tagged pUL47 of Marek's disease virus reveals differential tissue expression of the tegument protein in vivo. | Marek's disease virus (MDV), a lymphotropic alphaherpesvirus, causes Marek's disease (MD) in chickens. MD is characterized by neurological signs, chronic wasting, and T cell lymphomas that predominate in the visceral organs. MDV replicates in a highly cell-associated manner in vitro and in vivo, with infectious virus particles only being released from feather follicle epithelial (FFE) cells in the skin. Virus produced and shed from FFE cells allows transmission of MDV from infected to naïve chic ... | 2011 | 22190714 |
| deletion of the meq gene significantly decreases immunosuppression in chickens caused by pathogenic marek's disease virus. | marek's disease virus (mdv) causes an acute lymphoproliferative disease in chickens, resulting in immunosuppression, which is considered to be an integral aspect of the pathogenesis of marek's disease (md). a recent study showed that deletion of the meq gene resulted in loss of transformation of t-cells in chickens and a meq-null virus, rmd5δmeq, could provide protection superior to cvi988/rispens. | 2011 | 21205328 |
| interferon-γ influences immunity elicited by vaccines against very virulent marek's disease virus. | vaccination of chickens with herpesvirus of turkey (hvt) confers only partial protection against challenge with a very virulent marek's disease virus (mdv). here, we evaluated the ability of recombinant chicken interferon-gamma (rchifn-γ) to enhance protective efficacy of hvt against the very virulent mdv strain, rb1b. the bioactivity of ifn-γ expressed by a plasmid expression vector was confirmed by its ability to stimulate a chicken macrophage cell line (hd11) to produce nitric oxide (no) in v ... | 2011 | 21501630 |
| [marek's disease virus can infect chicken brain microglia and promote the transcription of toll-like receptor 15 and 1lb genes]. | microglial cells were purified from a mixed neuroglia culture prepared from the neonatal chicken brain in vitro, and were infected with the vvmdv yl040920 isolate and an attenuated mdv vaccine strain cvi988/rispens, respectively. the presence of cytopathic effect (cpe) was examined daily, and the meq expression in mdv-infected microglia was detected by immunohistochemistry assay. dna replication of the mdv meq gene and transcription of the gb gene were determined by real-time quantitative pcr (q ... | 2011 | 21462502 |
| artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of marek's disease virus (mdv) alters expression of nearby mdv genes. | researchers reported that co-cultivating the jm/102w strain of marek's disease virus (mdv) with reticuloendotheliosis virus (rev) resulted in an rev long terminal repeat (ltr) being inserted into the internal repeat short (irs) region of jm/102w. when the resulting recombinant virus was serially passed in cell culture, the initial ltr was duplicated and a second ltr spontaneously appeared in the terminal repeat short (trs) region of the mdv genome. the virus, designated rm1, was significantly at ... | 2011 | 21340512 |
| the ty3 gag3 spacer controls intracellular condensation and uncoating. | cells expressing the yeast retrotransposon ty3 form concentrated foci of ty3 proteins and rna within which virus-like particle (vlp) assembly occurs. gag3, the major structural protein of the ty3 retrotransposon, is composed of capsid (ca), spacer (sp), and nucleocapsid (nc) domains analogous to retroviral domains. unlike the known sp domains of retroviruses, ty3 sp is highly acidic. the current studies investigated the role of this domain. although deletion of ty3 sp dramatically reduced retrot ... | 2011 | 21270167 |
| molecular characteristics of polish field strains of marek's disease herpesvirus isolated from vaccinated chickens. | twenty-nine marek's disease virus (mdv) strains were isolated during a 3 year period (2007-2010) from vaccinated and infected chicken flocks in poland. these strains had caused severe clinical symptoms and lesions. in spite of proper vaccination with mono- or bivalent vaccines against marek's disease (md), the chickens developed symptoms of md with paralysis.because of this we decided to investigate possible changes and mutations in the field strains that could potentially increase their virulen ... | 2011 | 21320336 |
| effect of diluting marek's disease vaccines on the outcomes of marek's disease virus infection when challenged with highly virulent marek's disease viruses. | dilution of marek's disease (md) vaccines is a common practice in the field to reduce the cost associated with vaccination. in this study we have evaluated the effect of diluting md vaccines on the protection against md, vaccine and challenge md virus (mdv) kinetics, and body weight when challenged with strains md5 (very virulent mdv) and 648a (very virulent plus mdv) by contact at day of age. the following four vaccination protocols were evaluated in meat-type chickens: turkey herpesvirus (hvt) ... | 2011 | 21793444 |
| icp27 protein of marek's disease virus interacts with sr proteins and inhibits the splicing of cellular telomerase chtert and viral vil8 transcripts. | all herpesviruses have a post-transcriptional regulatory protein that prevents precursor mrna splicing and leads to the shutting off of host protein synthesis. the icp27 protein of herpes simplex virus 1 (hsv-1) is the prototype of these proteins. marek's disease virus (mdv-1), an alphaherpesvirus that induces lymphoma in birds, also has an icp27 protein that is produced in lytic mdv-1-infected cells. we characterized this protein. we demonstrated icp27 production in latently infected msb-1 cell ... | 2011 | 21325479 |
| critical role of the virus-encoded microrna-155 ortholog in the induction of marek's disease lymphomas. | notwithstanding the well-characterised roles of a number of oncogenes in neoplastic transformation, micrornas (mirnas) are increasingly implicated in several human cancers. discovery of mirnas in several oncogenic herpesviruses such as kshv has further highlighted the potential of virus-encoded mirnas to contribute to their oncogenic capabilities. nevertheless, despite the identification of several possible cancer-related genes as their targets, the direct in vivo role of virus-encoded mirnas in ... | 2011 | 21383974 |
| expression of ha of hpai h5n1 virus at us2 gene insertion site of turkey herpesvirus induced better protection than that at us10 gene insertion site. | herpesvirus of turkey (hvt) is being widely used as a vector for development of recombinant vaccines and us2 and us10 genes are often chosen as insertion sites for targeted gene expression. however, the different effects of the two genes for generation of recombinant hvt vaccines were unknown. in order to compare the effects of inserted genes in the two sites on the efficacy of the recombinant vaccines, host-protective haemagglutinin (ha) gene of the highly pathogenic avian influenza virus (hpai ... | 2011 | 21818336 |
| improving the potency of dna vaccine against chicken anemia virus (cav) by fusing vp1 protein of cav to marek's disease virus (mdv) type-1 vp22 protein. | studies have shown that the vp22 gene of marek's disease virus type-1 (mdv-1) has the property of movement between cells from the original cell of expression into the neighboring cells. the ability to facilitate the spreading of the linked proteins was used to improve the potency of the constructed dna vaccines against chicken anemia virus (cav). | 2011 | 21401953 |
| comparative analysis of oncogenic genes revealed unique evolutionary features of field marek's disease virus prevalent in recent years in china. | marek's disease (md) is an economically important viral disease of chickens caused by marek's disease virus (mdv), an oncogenic herpesvirus. this disease was well controlled since the widespread use of commercial vaccines, but field mdvs have shown continuous increasing in virulence and acquired the ability to overcome the immune response induced by vaccines. nowadays, md continues to be a serious threat to poultry industry, isolation and characterization of mdvs are essential for monitoring cha ... | 2011 | 21406076 |
| chromosomal integration of an avian oncogenic herpesvirus reveals telomeric preferences and evidence for lymphoma clonality. | abstract: | 2010 | 21429236 |
| expression profiles of micrornas encoded by the oncogenic marek's disease virus reveal two distinct expression patterns in vivo during different phases of disease. | marek's disease virus (mdv) is a long-recognized oncogenic herpesvirus, which induces lymphoma in its natural host that can be prevented by vaccination. mdv infection provides an excellent biological model for investigating the biology, genetics and immunology of viral oncogenesis. recently discovered micrornas (mirnas) in the mdv genome have been suggested to have regulatory roles during mdv oncogenesis. we have examined the expression profiles of all 22 previously reported mirnas encoded by md ... | 2010 | 21148277 |
| alternative splicing and nonsense-mediated decay regulate telomerase reverse transcriptase (tert) expression during virus-induced lymphomagenesis in vivo. | telomerase activation, a critical step in cell immortalization and oncogenesis, is partly regulated by alternative splicing. in this study, we aimed to use the marek's disease virus (mdv) t-cell lymphoma model to evaluate tert regulation by splicing during lymphomagenesis in vivo, from the start point to tumor establishment. | 2010 | 20964812 |
| functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (ltr) integrated into the genome of a field strain of marek's disease virus. | mdv-gx0101 is a field strain of marek's disease virus with a naturally occurring insertion of the reticuloendotheliosis virus (rev) ltr fragment. in order to study the biological properties of rev-ltr insertion in the mdv genome, we constructed a full-length infectious bac clone of mdv-gx0101 strain and deleted the ltr sequences by bac mutagenesis. the pathogenic properties of the ltr-deleted virus were evaluated in infected spf birds. the study demonstrated that the ltr-deleted virus had a stro ... | 2010 | 19962172 |
| [evaluation the immuno-protective effect of an infectious clone of meq- deleted marek's disease virus]. | to evaluate the immuno-protective effect of gx0101 deltameq-bac containing an infectious meq-null marek's disease virus genome. | 2010 | 20815243 |
| development of a primer-probe energy transfer based real-time pcr for detection of marek's disease virus. | a real-time pcr assay, which enables simultaneous detection and differentiation of all three serotypes of marek's disease virus, without the need for post-pcr sequencing, has been developed. the assay is based on the primer-probe energy transfer real-time pcr, which has a relatively high tolerance towards point mutations in the probe region. the pcr is followed by a probe melting point analysis, which enables confirmation of identity of amplicon and differentiation of serotypes. the assay target ... | 2010 | 20026120 |
| molecular epidemiological investigation of marek's disease virus from guangxi, china. | the predominant field strains of marek's disease virus in guangxi were clearly different from the vaccine strain cvi988/rispens based on sequencing of the envelope glycoprotein i (gi), glycoprotein e (ge) and oncogenic meq genes. these differences may be partly responsible for the most recent outbreaks in guangxi. | 2010 | 21053030 |
| transcriptome and proteome profiling of host responses to marek's disease virus in chickens. | marek's disease (md) is an immunosuppressive and proliferative disease of domestic chickens caused by a highly oncogenic cell-associated alpha-herpesvirus, named marek's disease virus (mdv). despite the availability of highly efficacious vaccines for control of md and existence of lines of chickens which display differential genetic susceptibility or resistance to this disease, little is known about the underlying mechanisms of mdv-host interactions. the recent advent of global or targeted gene ... | 2010 | 21067815 |
| immune responses against marek's disease virus. | it is more than a century since marek's disease (md) was first reported in chickens and since then there have been concerted efforts to better understand this disease, its causative agent and various approaches for control of this disease. recently, there have been several outbreaks of the disease in various regions, due to the evolving nature of md virus (mdv), which necessitates the implementation of improved prophylactic approaches. it is therefore essential to better understand the interacti ... | 2010 | 21087575 |
| expression profiling of genes associated with regulatory functions of t-cell subsets in marek's disease virus-infected chickens. | the environment of tumours caused by marek's disease virus (mdv) in chickens has been shown to have an immunoregulatory phenotype. the objective of the present study was to examine the expression of key t-regulatory markers during various stages of mdv pathogenesis. specific-pathogen free (spf) as well as major histocompatibility complex-defined chickens were infected with the rb1b and jm-16 strains of mdv, respectively. cd4(+) and cd8(+) t cells from the spleens of infected as well as age-match ... | 2010 | 20954013 |
| t-cell lymphoma in a wild okinawa rail (gallirallus okinawae). | the okinawa rail (gallirallus okinawae) is an endangered species that inhabits the northern part of okinawa main island in southern japan. a wild okinawa rail was rescued from a road in kunigami village in okinawa in october 2009. the bird subsequently died and underwent necropsy. tumors were found in the liver, spleen and part of the small intestine. microscopically, lymphoid neoplasm was confirmed in these tissues. the tumor cells were mainly positive for cd3 and cd8α by immunohistochemistry. ... | 2010 | 21060244 |
| the meq oncoprotein of marek's disease virus interacts with p53 and inhibits its transcriptional and apoptotic activities. | marek's disease virus (mdv) is an oncogenic herpesvirus, which causes malignant lymphoma in chickens. the meq protein of mdv, which is expressed abundantly in mdv-infected cells and in marek's disease (md) tumor cells, functions as a transcriptional activator and has been proposed to play an important role in oncogenic transformation. preliminary studies demonstrated that meq is able to bind p53 in vitro, as demonstrated using a protein-binding assay. this observation prompted us to examine whet ... | 2010 | 21110861 |
| pathogenicity of a very virulent strain of marek's disease herpesvirus cloned as infectious bacterial artificial chromosomes. | bacterial artificial chromosome (bac) vectors containing the full-length genomes of several herpesviruses have been used widely as tools to enable functional studies of viral genes. marek's disease viruses (mdvs) are highly oncogenic alphaherpesviruses that induce rapid-onset t-cell lymphomas in chickens. oncogenic strains of mdv reconstituted from bac clones have been used to examine the role of viral genes in inducing tumours. past studies have demonstrated continuous increase in virulence of ... | 2010 | 21127705 |
| the capacity of ul49.5 proteins to inhibit tap is widely distributed among members of the genus varicellovirus. | the lifelong infection by varicelloviruses is characterized by a fine balance between the host immune response and immune evasion strategies used by these viruses. virus-derived peptides are presented to cytotoxic t lymphocytes by major histocompatibility complex (mhc) class i molecules. the transporter associated with antigen processing (tap) transports the peptides from the cytosol into the endoplasmic reticulum, where the loading of mhc-i molecules occurs. the varicelloviruses bovine herpesvi ... | 2010 | 21159875 |
| cytogenetic stability of chicken t-cell line transformed with marek's disease virus: atomic force microscope, a new tool for investigation. | the marek's disease virus (mdv) integration may induce a novel organization of chromatin architecture with a modified genetic expression. in our opinion it is worthwhile trying to relate cytogenetic stability to functional modifications. recently, atomic force microscopy technique was applied to study the structure of chromosomes at a nanoscale level. this high resolution allows to investigate the different structure of chromatin in order to study cytogenetic stability and chromosome aberrations ... | 2010 | 21161951 |
| a meq-deleted marek's disease virus cloned as a bacterial artificial chromosome is a highly efficacious vaccine. | the marek's disease virus (mdv) induces t-cell tumors in susceptible chickens. of the 80 to 100 known mdv genes, only the mdv meq gene was shown to have transforming properties. further evidence that meq is probably the principal oncogene in mdv came when researchers used overlapping cosmid clones of mdv and demonstrated that deleting meq resulted in a highly protective marek's disease (md) vaccine. we deleted both copies of meq from a bacterial artificial chromosome clone (bac) of mdv. the viru ... | 2010 | 20608531 |
| down-regulation of mhc class i by the marek's disease virus (mdv) ul49.5 gene product mildly affects virulence in a haplotype-specific fashion. | marek's disease is a devastating neoplastic disease of chickens caused by marek's disease virus (mdv). mdv down-regulates surface expression of mhc class i molecules, although the mechanism has remained elusive. mdv harbors a ul49.5 homolog that has been shown to down-regulate mhc class i expression in other varicelloviruses. using in vitro assays, we showed that mdv pul49.5 down-regulates mhc class i directly and identified its cytoplasmic tail as essential for this function. in vivo, viruses l ... | 2010 | 20637486 |
| late marek's disease in adult chickens inoculated with virulent marek's disease virus. | recently, excessive losses from marek's disease (md) have been noted in adult laying flocks over the age of 40 weeks. we defined these late outbreaks in adult chickens as "late md", and experimentally reproduced the disease in adult spf p2 line (50-week-old) or commercial line (74-week-old) chickens inoculated with a virulent strain of marek's disease virus (mdv). commercial line chickens were given mdv vaccines (hvt and cvi 988) at hatch. the occurrence of md was evaluated periodically by the e ... | 2010 | 20651465 |
| marek's disease virus microrna designated mdv1-pre-mir-m4 targets both cellular and viral genes. | mdv1-mir-m4 is one of 25 micrornas (mirnas) expressed by marek's disease virus (mdv-1), an oncogenic alphaherpesvirus that induces fatal t-cell lymphoma in chickens. mdv1-mir-m4 was shown to be the second functional viral ortholog of mir-155, a cellular mirna that plays a crucial role in several physiological and pathological processes in lymphocyte biology. in this study, we investigated a panel of putative mdv1-mir-m4 targets involved in gene networks affecting both cellular and viral life cyc ... | 2010 | 20680360 |
| expression profiles of genes within a subregion of chicken major histocompatibility complex b in spleen after marek's disease virus infection. | major histocompatibility complex has previously been shown to influence the resistance of chicken to marek's disease virus (mdv). however, little is known about expression of other genes in the mhc-i and ii pathway after mdv infection. this study aimed at investigating 8 immune-related genes in the mhc core region that affects host responses to mdv. spleens of infected and age-matched uninfected chickens were removed at 4, 7, 14, 21, and 28 d postinfection for gene expression detection using rea ... | 2010 | 20852103 |
| proteomic analysis of the host response in the bursa of fabricius of chickens infected with marek's disease virus. | marek's disease virus (mdv) is an oncogenic herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. in this paper, we identified differentially expressed proteins in the bursa of fabricius of chickens infected with the highly virulent mdv strain rb1b, using two-dimensional gel electrophoresis on samples from time points coinciding with mdv life-cycle phases. by maldi-tof/tof mass spectrometer, 26 proteins were identified, including 8 persistently up- ... | 2010 | 20723570 |
| sequence analysis of meq oncogene among polish strains of marek's disease. | the main goal of the present study was to analyse the nucleotide and amino acid sequence of meq--the main oncoprotein of marek's disease virus. the conducted analysis of the nucleotide sequences of mdv has shown a significant similarity between polish and reference strains of mdv assigned to the vvmdv and vv+mdv pathotypes. some of the detected nucleotide point mutations were specific only for polish strains. the sequence analysis has shown a number of single substitutions and two poly-amino aci ... | 2010 | 20731180 |
| herpesvirus telomerase rna(vtr)-dependent lymphoma formation does not require interaction of vtr with telomerase reverse transcriptase (tert). | telomerase is a ribonucleoprotein complex involved in the maintenance of telomeres, a protective structure at the distal ends of chromosomes. the enzyme complex contains two main components, telomerase reverse transcriptase (tert), the catalytic subunit, and telomerase rna (tr), which serves as a template for the addition of telomeric repeats (ttaggg)(n). marek's disease virus (mdv), an oncogenic herpesvirus inducing fatal lymphoma in chickens, encodes a tr homologue, viral tr (vtr), which signi ... | 2010 | 20865127 |
| differential quantification of cloned cvi988 vaccine strain and virulent rb-1b strain of marek's disease viruses in chicken tissues, using real-time pcr. | the 'gold standard' vaccine against marek's disease in poultry is the cvi988/rispens virus, which is not easily distinguishable, antigenically or genetically, from virulent marek's disease herpesvirus. accurate differential measurement of the cvi988 vaccine and virulent viruses is important to investigate mechanisms of vaccinal protection. minimal sequence differences between cvi988 and virulent mdv strains restrict the application of molecular diagnostic methods such as real-time pcr to disting ... | 2010 | 20810138 |
| herpesviruses and chromosomal integration. | herpesviruses are members of a diverse family of viruses that colonize all vertebrates from fish to mammals. although more than one hundred herpesviruses exist, all are nearly identical architecturally, with a genome consisting of a linear double-stranded dna molecule (100 to 225 kbp) protected by an icosahedral capsid made up of 162 hollow-centered capsomeres, a tegument surrounding the nucleocapsid, and a viral envelope derived from host membranes. upon infection, the linear viral dna is deliv ... | 2010 | 20844040 |
| a p53-dependent promoter associated with polymorphic tandem repeats controls the expression of a viral transcript encoding clustered micrornas. | the tumor suppressor protein p53 plays a role in cellular responses to cancer-initiating events by regulating progress through the cell cycle. several recent studies have shown that p53 transactivates expression of the members of the proapoptotic microrna-34 family, which are underexpressed in several cancers. we demonstrate here that the latency-associated cluster of micrornas (mirna) encoded by an oncogenic herpesvirus, gallid herpesvirus 2 (gahv-2), is a direct target of p53. robust transcrip ... | 2010 | 20881002 |
| marek's disease virus-encoded micrornas: genomics, expression and function. | micrornas (mirnas) are the recently discovered small non-coding rna molecules that have post-transcriptional regulatory functions in many important biological processes. a large number of mirnas have been found to be encoded by viral genomes, especially in herpesviruses. previous research regarding mirnas encoded by herpesviruses, including marek's disease virus (mdv), has demonstrated their involvement in lytic replication, latent infection, t-lymphocyte transformation and tumorigenesis. mdv is ... | 2010 | 20953939 |
| marek's disease virus type 1 microrna mir-m3 suppresses cisplatin-induced apoptosis by targeting smad2 of the transforming growth factor beta signal pathway. | viruses cause about 15% of the cancers that are still the leading causes of human mortality. the discovery of viral oncogenes has enhanced our understanding of viral oncogenesis. however, the underlying molecular mechanisms of virus-induced cancers are complex and require further investigation. the present study has attempted to investigate the effects of the micrornas (mirnas) encoded by marek's disease virus 1 (mdv1), a chicken herpesvirus causing acute t-cell lymphomas and solid visceral tumo ... | 2010 | 20962090 |
| development, standardization and assessment of pcr systems for purity testing of avian viral vaccines. | the european pharmacopoeia (ph. eur.) requires avian viral vaccines to be free of adventitious agents. purity testing is an essential quality requirement of immunological veterinary medicinal products (ivmps) and testing for extraneous agents includes monitoring for many different viruses. conventional virus detection methods include serology or virus culture, however, molecular tests have become a valid alternative testing method. nucleic acid testing (nat) is fast, highly sensitive and has a h ... | 2010 | 20338785 |
| marek's disease virus influences the expression of genes associated with ifn-gamma-inducible mhc class ii expression. | chickens infected with marek's disease virus (mdv) become lifelong carriers regardless of their susceptibility to clinical disease. therefore various viral immune-evasive mechanisms must play a role in mdv-host interactions. mdv has previously been shown to influence the expression of major histocompatibility complex (mhc) class ii molecules. however, little is known about the underlying mechanisms of this phenomenon. in the present study, we studied the effect of mdv infection on the expression ... | 2010 | 20374003 |
| increased dna damage and oxidative stress in chickens with natural marek's disease. | oxidative stress contributes to the accumulation of genomic abnormalities, prevents cellular apoptosis, and also mediates immunosuppression resulting in tumor formation. marek's disease provides excellent opportunities for the study of herpesvirus-induced tumors both in experimental- and natural conditions. the aim of this study was to examine the effects of marek's disease (md) on basal levels of dna strand breaks and on the oxidative-antioxidative status of chickens with md. white-lohmann hens ... | 2010 | 19647879 |
| viral control of vtr expression is critical for efficient formation and dissemination of lymphoma induced by marek's disease virus (mdv). | marek's disease virus (mdv) is an alphaherpesvirus that causes lethal t-cell lymphomas in chickens. mdv is unique in that it harbors two copies of a viral telomerase rna subunit (vtr) in its genome exhibiting 88% sequence identity to the chicken orthologue, chtr. the minimal telomerase ribonucleoprotein complex consists of a protein subunit with reverse transcriptase activity (tert) and tr. physiologically, the complex compensates for the progressive telomere shortening that occurs during mitosi ... | 2010 | 20423696 |
| herpesviruses--a zoonotic threat? | herpesviruses are highly host specific and share a long synchronous evolution with their hosts. only in rare cases, species barriers fall and allow animal to human or human to animal transmission. among the zoonotic herpesviruses, cercopithecine herpesvirus 1 is the most significant and can be transmitted from macaques to human. conversely, human herpesvirus 1 is capable of causing severe disease in primates. besides those two examples, there are several herpesviruses with a certainly limited or ... | 2010 | 19616388 |
| [protective immunity of a meq-deleted marek's disease virus against very virulent virus challenge in chickens]. | to evaluate the pathogenicity and immunoprotective effect of a wild strain of marek's disease virus (mdv) after deleting of its meq oncogene. | 2010 | 20499644 |
| role of marek's disease herpesvirus in the induction of tumours in japanese quail (coturnix coturnix japonica) by methylcholanthrene. | the qt35 cell line, established from 20-methylcholanthrene (mca)-induced tumours in japanese quail, is positive for marek's disease virus (mdv), and therefore we examined whether mdv is important for the development of mca-induced tumours. japanese quail were inoculated with the jm16 strain of mdv at 1 or 3 days of age or left uninoculated. at 3 weeks of age, quail were injected in the breast muscle with 4 mg mca in corn oil or corn oil alone. quail were observed for tumours three times/week and ... | 2010 | 20544424 |
| vaccine-induced host responses against very virulent marek's disease virus infection in the lungs of chickens. | the aim of this study was to investigate the kinetics of virus replication and cellular responses in the lungs following infection with marek's disease virus (mdv) and/or vaccination with herpesvirus of turkey (hvt) via the respiratory route. chickens vaccinated with hvt and challenged with mdv had a higher accumulation of mdv and hvt genomes in the lungs compared to the chickens that received hvt or mdv alone. this increase in virus load was associated with augmented expression of interferon (i ... | 2010 | 20600510 |
| molecular and biological characterization of a marek's disease virus field strain with reticuloendotheliosis virus ltr insert. | a marek's disease virus (mdv) field strain designated gx0101 was isolated from a layer flock and confirmed to be a recombinant virus with an insert of a long terminal repeat (ltr) from the reticuloendotheliosis virus (rev). a chimeric molecule containing an rev-ltr insert of 539 bp and its flanking sequences from mdv was amplified and sequenced. an rev-ltr downstream from the internal repeat short (irs) region has 77.4-98.6% homology to seven rev field strains isolated from different avian speci ... | 2010 | 20043197 |
| identification of bzip interaction partners of viral proteins hbz, meq, bzlf1, and k-bzip using coiled-coil arrays. | basic-region leucine-zipper transcription factors (bzips) contain a segment rich in basic amino acids that can bind dna, followed by a leucine zipper that can interact with other leucine zippers to form coiled-coil homo- or heterodimers. several viruses encode proteins containing bzip domains, including four that encode bzips lacking significant homology to any human protein. we investigated the interaction specificity of these four viral bzips by using coiled-coil arrays to assess self-associat ... | 2010 | 20102225 |
| marek's disease resistant/susceptible mhc haplotypes in xiayan chickens identified on the basis of blb2 pcr-rflp and blb2/bf2 sequence analyses. | 1. the aim was to analyse the variability of the blb2/bf2 genes of xiayan chickens to identify homozygous birds with resistance or susceptibility to marek's disease (md). 2. the experiment used two lines: birds from a common line were divided into group a (unvaccinated) and group b (vaccinated with herpesvirus of turkeys (hvt)); and birds from an md-resistant line were divided into group c (unvaccinated) and group d (vaccinated with hvt). they were challenged intra-abdominally with marek's disea ... | 2010 | 20924848 |
| the effect of the time interval between exposures on the susceptibility of chickens to superinfection with marek's disease virus. | marek's disease virus (mdv) is ubiquitous within commercial poultry flocks because current vaccines do not prevent mdv infection or transmission. in order for newly-evolved mdv strains to become established within a flock, it seems inevitable that any new strain would need to infect and replicate in chickens previously infected with resident mdv strains. this phenomenon is difficult to detect and there is no clear evidence that it is even possible. four experiments were performed to demonstrate ... | 2010 | 20945786 |
| acute paretic syndrome in juvenile white leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. | sudden limb paresis is a common problem in white leghorn flocks, affecting about 1% of the chicken population before achievement of sexual maturity. previously, a similar clinical syndrome has been reported as being caused by inflammatory demyelination of peripheral nerve fibres. here, we investigated in detail the immunopathology of this paretic syndrome and its possible resemblance to human neuropathies. | 2010 | 20109187 |
| accumulation of attenuating mutations in varying proportions within a high passage very virulent plus strain of gallid herpesvirus type 2. | marek's disease (md) is controlled through mass vaccination. although these vaccines reduce or delay tumor formation they fail to induce sterilizing immunity and prevent virus shedding. relatively little is known about the genetic changes that lead to attenuation. it has been established that serial passage of virulent strains in avian cell lines results in the generation of attenuated progeny at some undefined passage level. a detailed cataloging of the mutations needed for attenuation will be ... | 2010 | 20109506 |
| both homo and heterodimers of marek's disease virus encoded meq protein contribute to transformation of lymphocytes in chickens. | marek' disease virus serotype-1, also know as gallid herpesvirus 2 (gahv-2), elicits t-cell lymphomas in chickens. the gahv-2 genome encodes an oncoprotein, meq, with similarity to the jun/fos family of proteins. we have previously shown that meq homodimers are not sufficient to induce lymphomas in chickens. in this study, we investigated the role of meq heterodimers in the pathogenicity of gahv-2 by generating a chimeric meq gene, which contains the leucine zipper region of fos (meqfos). a reco ... | 2010 | 20137800 |
| proteomic analysis of host responses to marek's disease virus infection in spleens of genetically resistant and susceptible chickens. | resistance to marek's disease (md) in chickens is genetically regulated and there are lines of chickens with differential susceptibility or resistance to this disease. the present study was designed to study comparative changes in the spleen proteomes of md-susceptible b19 and md-resistant b21 chickens in response to mdv infection. spleen proteomes were examined at 4, 7, 14 and 21 days post-infection (d.p.i.) using two-dimensional gel electrophoresis and subsequently the protein spots were ident ... | 2010 | 20138080 |
| deletion of 1.8-kb mrna of marek's disease virus decreases its replication ability but not oncogenicity. | the 1.8-kb mrna was reported as one of the oncogenesis-related genes of marek's disease virus (mdv). in this study, the bacterial artificial chromosome (bac) clone of a mdv field strain gx0101 was used as the platform to generate mutant mdv to examine the functional roles of 1.8-kb mrna. | 2010 | 21029474 |
| marek's disease viruses lacking either r-lorf10 or lorf4 have altered virulence in chickens. | the marek's disease virus (mdv, gallid herpesvirus 2) genome encodes approximately 110 open reading frames (orfs). many of these orfs are annotated based purely on homology to other herpesvirus genes, thus, direct experiments are needed to verify the gene products, especially the hypothetical or mdv-specific orfs, and characterize their biological function, particularly with respect to pathogenicity in chickens. previously, a comprehensive two-hybrid assay screen revealed nine specific chicken-m ... | 2010 | 20229182 |
| protection of chickens, with or without maternal antibodies, against ibdv infection by a recombinant ibdv-vp2 protein. | the use of avian herpesviruses (marek's disease virus, mdv) as vectors to express the capsid protein of infectious bursal disease virus (ibdv) was well established, and its protection against ibdv challenge has been evaluated previously. however, there is little data about rmdv1 expressing the vp2 protein of ibdv protecting spf and commercial chickens against virulent ibdv (vibdv) challenge. in this study, we constructed a stable rmdv1 expressing the vp2 protein of ibdv by inserting the coding s ... | 2010 | 20338216 |
| pathogenicity of a quail (coturnix coturnix japonica)-derived marek's disease virus rescued from the qt35 cell line. | the qt35 cell line was established in 1977 from methylcholanthrene-induced tumors in japanese quail. it was later shown that at least some of the qt35 cell lines were latently infected with marek's disease (md) virus (mdv). an mdv-like herpesvirus, named quail mdv (qmdv), was isolated from qt35 cells in 2000 by yamaguchi et al. to determine the pathogenicity of qmdv, we inoculated 10-day-old specific-pathogen-free chickens with qmdv jm (virulent), rb-1b (very virulent), or 584a (very virulent pl ... | 2010 | 20408411 |
| microrna mir-155 inhibits bone morphogenetic protein (bmp) signaling and bmp-mediated epstein-barr virus reactivation. | microrna mir-155 is expressed at elevated levels in human cancers including cancers of the lung, breast, colon, and a subset of lymphoid malignancies. in b cells, mir-155 is induced by the oncogenic latency gene expression program of the human herpesvirus epstein-barr virus (ebv). two other oncogenic herpesviruses, kaposi's sarcoma-associated herpesvirus and marek's disease virus, encode functional homologues of mir-155, suggesting a role for this microrna in the biology and pathogenesis of thes ... | 2010 | 20427544 |
| development of a loop-mediated isothermal amplification method for rapid detection of reticuloendotheliosis virus. | a loop-mediated isothermal amplification (lamp) method for rapid detection of reticuloendotheliosis virus (rev) was developed. the method used a set of two pairs of primers to amplify the pol gene for detecting rev, showing high specificity and sensitivity. the rev lamp method did not cross-react with common avian dna viruses (marek's disease virus, chicken anaemia virus, avian leucosis virus of subgroup j). additionally, the assay could detect different rev strains and had a detection limit of ... | 2010 | 20435068 |
| virulent marek's disease virus generated from infectious bacterial artificial chromosome clones with complete dna sequence and the implication of viral genetic homogeneity in pathogenesis. | genetic homogeneity of a test population is essential to precisely associate a viral genome sequence and its phenotype at the nucleotide level. however, homogeneity is not easy to achieve for marek's disease virus (mdv) due to its strictly cell-associated replication. to address this problem, two virulent infectious bacterial artificial chromosome (bac) clones of mdv were generated from an mdv genome previously cloned as five overlapping cosmids. the md5sn5bac clone has the bac vector inserted b ... | 2010 | 21123546 |
| microrna-26a-mediated regulation of interleukin-2 expression in transformed avian lymphocyte lines. | abstract: | 2010 | 20441582 |
| marek's disease virus vp22 enhances potentially the immune response of esat-6/cfp-10 against mycobacterium bovis infection. | currently available vaccines against mycobacterium bovis, the causative agent of tuberculosis, do not provide reliable efficacy and there is therefore a need for a novel vaccine with improved efficacy. here, we use protein transduction technology to deliver dna vaccines expressing mycobacterial antigens directly to target cells. we used various protein transduction domain (ptd) proteins including the vp22 conjugate from marek's disease virus serotype 1 (mdv-1), as delivery systems for dna constr ... | 2010 | 20458447 |
| further analysis of marek's disease virus horizontal transmission confirms that u(l)44 (gc) and u(l)13 protein kinase activity are essential, while u(s)2 is nonessential. | marek's disease virus (mdv) causes a devastating disease in chickens characterized by the development of lymphoblastoid tumors in multiple organs and is transmitted from the skin of infected chickens. we have previously reported that the u(s)2, u(l)44 (glycoprotein c [gc]), and u(l)13 genes are essential for horizontal transmission of mdv in gain-of-function studies using an a priori spread-deficient virus that was based on an infectious clone from the highly virulent rb-1b virus (prb-1b). to pr ... | 2010 | 20484497 |
| marek's disease virus-induced immunosuppression: array analysis of chicken immune response gene expression profiling. | marek's disease (md) is a lymphoproliferative disease of chickens induced by a highly cell-associated oncogenic alpha-herpesvirus, marek's disease virus (mdv). mdv replicates in chicken lymphocytes and establishes a latency infection within cd4(+) t cells. host-virus interaction, immune responses to infection, and transcriptional profiling of chicken gene expression in md are poorly understood. in this study we conducted a global host gene expression analysis in the splenocytes of mdv-infected c ... | 2010 | 20565294 |
| a novel protein-coding orf72.2 gene was identified from marek's disease virus strain cvi988. | marek's disease is a highly contagious disease of poultry characterized by rapid-on set of t-cell lymphomas, which is caused by marek's disease virus (mdv), but its pathogenic mechanism is still not very clear. recently, some new progress were achieved in molecular character of mdv. along with the genomic sequencing of mdv serotype 1, some novel open reading frames (orfs) were predicted, and orf72.2 was one of them which have no homologues in other mdv serotypes or in other alphaherpesvirus. in ... | 2010 | 21176240 |
| comparative evaluation of vaccine efficacy of recombinant marek's disease virus vaccine lacking meq oncogene in commercial chickens. | marek's disease virus (mdv) oncogene meq has been identified as the gene involved in tumorigenesis in chickens. we have recently developed a meq-null virus, rmd5 delta meq, in which the oncogene meq was deleted. vaccine efficacy experiments conducted in avian disease and oncology laboratory (adol) 15i(5) x 7(1) chickens vaccinated with rmd5 delta meq virus or an adol preparation of cvi988/rispens indicated that rmd5 delta meq provided superior protection than cvi988/rispens when challenged with ... | 2010 | 19941987 |
| comparative efficacy of bac-derived recombinant sb-1 vaccine and the parent wild type strain in preventing replication, shedding and disease induced by virulent marek's disease virus. | a widely used vaccine against marek's disease (md) in poultry is the virus sb-1, which is antigenically-related to the causative agent, marek's disease herpesvirus. we recently cloned the sb-1 genome as an infectious bacterial artificial chromosome, bac, (psb-1). the protective efficacies and replication kinetics of psb-1 and the parent strain (sb-1) were compared in an experimental model of md induced by a virulent strain, rb-1b. although vaccine virus replication and shedding was lower for psb ... | 2010 | 20144837 |
| [issues on evolution of cpg islands in genome of marek's disease virus]. | to study the hypothesis about influence of one form of epigenetic heredity--dna metylation--on several criteria of pathogenicity of avian herpesviruses using marek's disease virus (mdv) as a model. | 2010 | 20218351 |
| latency of marek's disease virus (mdv) in a reticuloendotheliosis virus-transformed t-cell line. ii: expression of the latent mdv genome. | marek's disease virus (mdv) is an alphaherpesvirus of chickens that causes the paralysis and rapid lymphoma formation known as marek's disease. mdv establishes latent infection in activated cd4+ t-cells, and these cells are also the target for transformation. mdv latency has been studied using mdv lymphoma-derived cell lines and t-cells isolated from infected chickens. each of these models has limitations because mdv-transformed cell lines require the use of oncogenic viruses; conversely, pools ... | 2009 | 19630218 |
| a deletion within glycoprotein l of marek's disease virus (mdv) field isolates correlates with a decrease in bivalent mdv vaccine efficacy in contact-exposed chickens. | we examined the functional role of a naturally occurring deletion within the glycoprotein l (gl) gene of marek's disease virus (mdv) field isolates. we previously showed that this mutation incrementally increased the virulence of an mdv in contact-exposed spf leghorn chickens, when chickens shedding this virus were co-infected with herpesvirus of turkeys (hvt). in our present study, we examined this mutation using two stocks of the very virulent plus (vv+)mdv strain tk, one of which harbored thi ... | 2009 | 19630238 |
| [marek's disease virus encoded mirnas--an update review]. | 2009 | 19678572 | |
| the construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mrna transcripts of marek's disease viruses. | marek's disease virus (mdv) has a bi-directional promoter between pp38 gene and 1.8-kb mrna transcripts. by sequencing for the promoters from 8 different strains (cvi988, 814, ga, jm, md5, g2, rb1b and 648a), it is found, comparing with the other 7 mdv strains, cvi988 has a 5-bp (from -628 to -632) deletion in this region, which caused a sp1 site destroyed. in order to analysis the activity of the promoter, the complete bi-directional promoters from ga and cvi988 were, respectively, cloned into ... | 2009 | 19948021 |
| identification of an intercistronic internal ribosome entry site in a marek's disease virus immediate-early gene. | in this study, we have identified an internal ribosome entry site (ires) from the highly infectious herpesvirus marek's disease virus (mdv). the ires was mapped to the intercistronic region (icr) of a bicistronic mrna that we cloned from the mdv-transformed cd4(+) t-cell line msb-1. the transcript is a member of a family of mrnas expressed as immediate-early genes with two open reading frames (orf). the first orf encodes a 14-kda polypeptide with two n-terminal splice variants, whereas the secon ... | 2009 | 19297480 |
| interaction of marek's disease virus oncoprotein meq with heat-shock protein 70 in lymphoid tumour cells. | marek's disease virus (mdv) is a highly oncogenic alphaherpesvirus that induces the rapid onset of t-cell lymphomas in poultry. the mdv-encoded oncoprotein meq plays an important role in oncogenicity, as its deletion abolishes the ability of the virus to induce tumours. it has been shown previously that meq oncogenicity is linked to its interaction with c-terminal binding protein 1 (ctbp), a property also shared by other virus-encoded oncoproteins such as adenovirus e1a and epstein-barr virus eb ... | 2009 | 19494050 |
| differential expression of micrornas in marek's disease virus-transformed t-lymphoma cell lines. | micrornas (mirnas) are increasingly recognized to play crucial roles in regulation of gene expression in different biological events, including many sporadic forms of cancer. however, despite the involvement of several viruses in inducing cancer, only a limited number of studies have been carried out to examine the mirna expression signatures in virus-induced neoplasia, particularly in herpesvirus-induced tumours where virus-encoded mirnas also contribute significantly to the mirnome of the tumo ... | 2009 | 19297609 |
| micrornas of gallid and meleagrid herpesviruses show generally conserved genomic locations and are virus-specific. | many herpesviruses, including marek's disease viruses (mdv1 and mdv2), encode micrornas. in this study, we report micrornas of two related herpesviruses, infectious laryngotracheitis virus (iltv) and herpesvirus of turkeys (hvt), as well as additional mdv2 micrornas. the genome locations, but not microrna sequences, are conserved among all four of these avian herpesviruses. most are clustered in the repeats flanking the unique long region (i/tr(l)), except in iltv which lacks these repeats. two ... | 2009 | 19328516 |
| factors influencing the attenuation of serotype 1 marek's disease virus by serial cell culture passage and evaluation of attenuated strains for protection and replication. | this study was carried out to better understand factors that influenced the process of attenuation of marek's disease (md) virus by serial passage in cell cultures. three virulent (v) pathotype and three very virulent plus (vv+) pathotype strains were passed by three techniques up to 131 times, and the passage level at attenuation was determined. the 18 attenuated or partially attenuated viruses were evaluated for protection against challenge with virulent md virus, and the virus load (latent in ... | 2009 | 19432005 |
| analyses of the spleen proteome of chickens infected with marek's disease virus. | marek's disease virus (mdv), which causes a lymphoproliferative disease in chickens, is known to induce host responses leading to protection against disease in a manner dependent on genetic background of chickens and virulence of the virus. in the present study, changes in the spleen proteome at 7, 14 and 21 days post-infection in response to mdv infection were studied using two-dimensional polyacrylamide gel electrophoresis. differentially expressed proteins were identified using one-dimensiona ... | 2009 | 19540544 |
| validation of marek's disease diagnosis and monitoring of marek's disease vaccines from samples collected in fta cards. | the use of flinders technology associates (fta) filter cards to quantify marek's disease virus (mdv) dna for the diagnosis of marek's disease (md) and to monitor md vaccines was evaluated. samples of blood (43), solid tumors (14), and feather pulp (fp; 36) collected fresh and in fta cards were analyzed. mdv dna load was quantified by real-time pcr. threshold cycle (ct) ratios were calculated for each sample by dividing the ct value of the internal control gene (glyceraldehyde-3-phosphate dehydro ... | 2009 | 20095150 |
| molecular characterization of the genome of duck enteritis virus. | the genomic sequence of a strain of duck enteritis virus (dev) was determined and analyzed in this study. the size of its genome is 158,091 bp in length and the genome is predicted to encode 78 putative proteins and resembles the members of the alphaherpesvirinae in genomic organization and gene composition. the genome of the virus is composed of a unique long (ul) region, a unique short (us) region, a unique short internal repeat (irs) region and a unique short terminal repeat (trs) region. its ... | 2009 | 19595405 |
| cytokine gene expression in splenic cd4+ and cd8+ t cell subsets of genetically resistant and susceptible chickens infected with marek's disease virus. | t cells from the spleens of b(19)/b(19) and b(21)/b(21) chickens infected with mdv jm-16 strain were fractionated by flow cytometry at 4, 10, 21 days post infection (d.p.i.). the expression of cytokine and viral genes (meq and glycoprotein b (gb)) was measured by real-time rt-pcr. it was determined that cd4(+) and cd8(+) t cells had both become infected with marek's disease virus (mdv) in both chicken lines. there was significantly higher expression of meq in cd4(+) t cells compared to cd8(+) t ... | 2009 | 19615758 |
| construction of an infectious marek's disease virus bacterial artificial chromosome and characterization of protection induced in chickens. | marek's disease virus (mdv) is a highly oncogenic alphaherpesvirus that induces rapid-onset t-cell lymphoma in poultry. the complete genome of the avirulent vaccine strain mdv-814 was cloned as an infectious bacterial artificial chromosome (bac) using an 8.8-kb fragment containing the self-designed selective marker guanosine phosphoriboxyl transferase. the recombinant virus mdv-814-bac was generated by co-transfection of a bac transfer vector and mdv-814 total dna, and was purified by eight roun ... | 2009 | 19026690 |